80 results about "Pyrrovinyquinium" patented technology

The invention discloses an endogenous anti-aging composition based on an internal and external synergistic mechanism and a preparation method of the endogenous anti-aging composition. The endogenous anti-aging composition based on the internal and external synergistic mechanism is prepared from the following components in parts by mass: 100 to 200 parts of alpha-ketoglutarate calcium; 100 to 200 parts of an olive leaf extract; 10 to 20 parts of nicotinic acid; 10 to 20 parts of pyrroloquinoline quinone; 20 to 60 parts of pomegranate extract; and 10 to 30 parts of coenzyme Q10. The composition and the preparation method thereof have the beneficial effects that the composition capable of integrally improving the aging state of women from multiple dimensions and the preparation method of the composition are provided.

The invention discloses a health-care food for delaying ovarian aging, which belongs to the technical field of health-care foods and comprises a composition for improving the levels of anti-mullerian test tube hormone and superoxide dismutase and functional auxiliary materials, the composition for improving the anti-muller test tube hormone and superoxide dismutase level is prepared from the following components in parts by weight: 2 to 5 parts of pyrroloquinoline quinone PQQ, 30 to 80 parts of cranberry powder and 40 to 100 parts of an isoflavone extract, and the content of the pyrroloquinoline quinone PQQ is 2 to 5 parts, the content of the cranberry powder is 30 to 80 parts, and the content of the isoflavone extract is 40 to 100 parts. The pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract in a specific formula have the effect of synergistically improving the levels of anti-muller test tube hormone and superoxide dismutase, and the health food prepared from the pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract can improve sleep, improve urinary tract infection of middle-aged women, delay ovarian aging and the like.

The invention discloses an anti-aging composition which comprises the following components or consists of the following components in parts by weight: 10-20 parts of pyrroloquinoline quinone or derivatives thereof; 2 to 30 parts by weight of ergothioneine; 0.2 to 10 parts by weight of spermidine; and 10-650 parts by weight of a pomegranate extract. The invention also discloses an application of the anti-aging composition. According to the anti-aging composition disclosed by the invention, the pyrroloquinoline quinone or the derivative thereof, the ergothioneine, the spermidine and the pomegranate extract contained in the anti-aging composition can generate a synergistic effect, so that the anti-aging composition has good anti-oxidation and anti-aging effects. The anti-aging composition can be applied to food, health care products and medicines, and has the effects of resisting skin oxidation and delaying aging.

The invention relates to a composition with bacteriostatic, anti-inflammatory and / or oral cavity repairing effects and application of the composition in toothpaste, and belongs to the technical field of daily chemicals. The composition disclosed by the invention is prepared from the following components in parts by weight: 0.04 to 0.06 part of pyrroloquinoline quinone and 0.2 to 0.3 part of carboxymethyl chitosan. Based on the anti-oxidative stress regulation and control capability of pyrroloquinoline quinone and the broad-spectrum antibacterial synergistic effect of carboxymethyl chitosan, pyrroloquinoline quinone and carboxymethyl chitosan are compounded and applied to a toothpaste formula system, so that the toothpaste has the effects of inhibiting bacteria, resisting inflammation, proliferating and repairing gingival fibroblasts and regulating the composition and diversity of oral microorganisms. According to the composition disclosed by the invention, through a multi-mechanism synergistic effect, an oral health maintenance system integrating bacteriostatic protection, inflammation regulation and control and mucous membrane repair is constructed, and the compound requirements of modern consumers on oral microenvironment regulation and control, mucous membrane barrier strengthening and long-term health management are accurately met.

The invention relates to the field of PQQ production, in particular to an engineering bacterium for producing pyrroloquinoline quinone and a preparation method of the engineering bacterium. According to the invention, different broad-host shuttle vectors are used, PQQ is expressed in denitrifying hyphomicrobe to synthesize related gene clusters, denitrifying hyphomicrobe engineering bacteria with high yield of PQQ are successfully constructed, and the engineering bacteria have the characteristics of rapid growth, high fermentation yield and the like, and lay a foundation for realizing green production of PQQ.

The invention discloses a composition containing NMN and a preparation method and application thereof. The composition provided by the invention comprises any two or more of NMN, coenzyme Q10, pyrroloquinoline quinone, resveratrol, plant fiber and glutathione. The invention unexpectedly discovers that the combination of two or more of NMN, coenzyme Q10, pyrroloquinoline quinone, resveratrol, plant fiber, glutathione and gamma-butylamine shows a beneficial synergistic effect in improving the NAD + level in vivo compared with a single component only applying pure NMN. Moreover, this synergy may result in additional benefits in therapy, such as anti-aging, enhancing immunity, resisting oxidation, protecting nerves, improving sub-health status, improving patient compliance, and the like. Meanwhile, no side effect is generated after long-term taking, and the conditions of body tolerance, drug effect absorption reduction and the like caused by long-term taking are also avoided.

The invention relates to the technical field of microorganisms, in particular to application of lactobacillus rhamnosus TG013 in preparation of an antioxidant product, the preservation number of the lactobacillus rhamnosus TG013 is CCTCC (China Center For Type Culture Collection) NO: M 2023036, and the antioxidant product comprises at least one of pyrroloquinoline quinone, nicotinic acid and an enzymatic antioxidant.

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

The application discloses application of pyrroloquinoline quinone (PQQ) or a derivative thereof in preparation of a product for relieving or preventing blue-green algae toxin poisoning of yellow catfish, and belongs to the technical field of aquatic animal feed. The PQQ or the derivative thereof can be used as an aquatic feed additive or a medicine, and is added in an amount of 3-6 mg / kg of feed to feed yellow catfish, so as to effectively relieve pathological damage of the liver of the yellow catfish caused by blue-green algae toxin through various ways such as improving key enzyme activity of liver function, inhibiting liver oxidative stress, relieving inflammatory reaction and reducing structural damage of tissues. The PQQ is a new food raw material approved in China in 2022, has advantages of high safety, good water solubility, moderate cost, similar dosage to that of feed vitamins and the like, and provides a safe, economical and convenient new nutritional intervention strategy for blue-green algae toxin poisoning of the yellow catfish, and has a wide industrial application prospect.

The utility model relates to a pyrrole quinolinone production technical field especially relates to a kind of recovery treatment system for pyrrole quinolinone salting-out mother liquor and crystallization mother liquor, including pyrrole quinolinone salting-out mother liquor conveying pipeline and pyrrole quinolinone crystallization mother liquor conveying pipeline, pyrrole quinolinone salting-out mother liquor conveying pipeline, first concentration kettle, first crystallization, first drying device, sodium chloride storage tank and series connection;Pyrrole quinolinone crystallization mother liquor conveying pipeline, second concentration kettle, dilution kettle, decoloring kettle, first resin column, first pH adjusting kettle, second resin column, second pH adjusting kettle, second crystallization kettle, second drying device, pyrrole quinolinone storage tank are connected in series in proper order.The above-mentioned recovery treatment system design is reasonable, can recycle and utilize sodium chloride in salting-out mother liquor and pyrrole quinolinone in crystallization mother liquor, avoids resource waste.

To provide an easy and convenient method with fewer steps that uses a reaction which can be completed in a shorter time, the method capable of suppressing as much as possible the mixing of the raw material pyrroloquinoline quinone into the product and the formation of impurities causing difficulty in purification, thereby enabling inexpensive mass production of imidazopyrroloquinoline alkali metal salts with higher purity.SOLUTION: A method for producing an imidazopyrroloquinoline alkali metal salt comprises reacting, in an aqueous solution, pyrroloquinoline quinone or a salt thereof at a concentration of 10 to 50 g / L in the presence of glycine or a salt thereof and a basic alkali metal salt so as to precipitate an imidazopyrroloquinoline alkali metal salt represented by the general formula (1) in the figure.SELECTED DRAWING: None

The invention relates to the technical field of rice herbicide phytotoxicity prevention and control, in particular to a method for relieving rice herbicide phytotoxicity in the seedling stage. Paddy rice seeds are soaked with a pyrroloquinoline quinine solution before sowing; the concentration of pyrroloquinoline quinine in the pyrroloquinoline quinine solution is 0.3 to 0.7 [mu] mol / L; the method comprises the following steps: spraying a pyrroloquinoline quinine solution on leaf surfaces in a period from two leaves to one core to three leaves of rice, wherein the concentration of pyrroloquinoline quinine in the pyrroloquinoline quinine solution is 1-2 [mu] mol / L; within 3-7 days after the pyrroloquinoline quinone solution is sprayed on the rice leaf surfaces, cyclopropanesulfonamide and a herbicide are mixed for use. PQQ and cyclopropanesulfonamide are coordinated to effectively relieve the phytotoxicity of herbicides to rice.

The invention relates to a composition for repairing DNA and delaying senescence and a preparation method and application thereof.The composition is prepared from, by mass, 10%-30% of rhodiola rosea extract, 5%-15% of ergothioneine, 1%-5% of pyrroloquinoline quinone (PQQ), 5%-20% of acai extract, 0.1%-3% of astaxanthin, 0.5%-5% of lycopene, 0.1%-2% of vitamin B2, 0.01%-0.5% of vitamin B12 and the balance water. The balance is a pharmaceutically or food acceptable carrier. The preparation method comprises the following steps: step 1, mixing the rhodiola rosea extract, the ergothioneine, the pyrroloquinoline quinone (PQQ), the acai extract, the astaxanthin and the lycopene in proportion; step 2, adding vitamins B2 and B12, and carrying out homogenization treatment; and step 3, adding a pharmaceutically or food acceptable carrier to prepare a target dosage form. The composition realizes an anti-aging effect at a cell DNA gene level through a targeted DNA repair pathway and in combination with a multi-component synergistic effect mechanism.

Disclosed are a self-emulsifying composition of a coenzyme Q10 with high bioavailability, and a method and device for preparing the same. The composition includes the following components in percentage by weight: 5-35% of a coenzyme Q10, 1-50% of an oil phase, 10-60% of a surfactant, 1-10% of a cosurfactant, 0-7% of an antioxidant, and 0.5-2% of pyrroloquinoline quinone disodium salt. The coenzyme Q10 medicinal liquid obtained can be kept in a clear and transparent solution state at room temperature, can be stably dispersed in water and self-emulsified to a transparent emulsion, significantly improves the bioavailability of the coenzyme Q10 by more than 4 times, and can be filled in capsules or softgels to swallow. The method disclosed by the disclosure includes two steps: material grinding and vacuum self-emulsifying. The method is concise in step and easy to control. Moreover, the device is simple in structure and low in cost.

Provided is a formulation that can prevent or treat pregnancy hypertension syndrome. A prevention or treatment agent for pregnancy hypertension syndrome is prepared, said agent containing an effective amount of one or more substances selected from the group consisting of pyrroloquinoline quinone, salts of pyrroloquinoline quinone, apple polyphenols, black rice, and black rice extracts.

The invention discloses a traditional Chinese medicine and non-traditional Chinese medicine combination formula for treating sub-health, and relates to the technical field of combination of traditional Chinese medicines and nutritional supplements.The traditional Chinese medicine and non-traditional Chinese medicine combination formula comprises 200 mg of lipoic acid, 100 mg of coenzyme Q10, 20 mg of pyrroloquinoline quinone, 3 g of xylooligosaccharide, 5 g of glutamine, 400 mg of plant lactic acid bacteria, 5 billion CFC of bifidobacterium longum, 300 mg of South African drunken solanum, 100 mg of rhodiola rosea, 200 mg of semen vaccariae extract, 50 mg of centella asiatica extract and 100 mg of phosphatidylserine. Compared with the prior art, the invention has the following advantages: the drug effect is stable; the effect is quick; the functions are comprehensive; the safety is high; the dosage forms are various.

The invention belongs to the technical field of biology, and provides a paracoccus Wheatstone strain and application thereof, the paracoccus Wheatstone strain can produce pyrroloquinoline quinine at high yield, the strain is named as YJY25-02, and the preservation number is CGMCC NO.34258. The strain is subjected to shake-flask aerobic culture for 83h, and the titer of the pyrroloquinoline quinone in fermentation liquor is 176.7 mg / L; the titer of pyrroloquinoline quinine in fermentation liquor during aerobic culture in a fermentation tank is 1796-1863 mg / L, the methanol conversion rate is 16.19-18.12 mg / g, the method has the advantages of short fermentation time, high titer and high methanol conversion rate, and the problem of large-scale production can be solved; pure pyrroloquinoline quinine with the purity larger than or equal to 98% is prepared through separation and purification of fermentation liquor, the yield ranges from 80% to 85%, the high yield and high purity of the pure pyrroloquinoline quinine are achieved, the separation and purification process is simple, the cost is low, organic solvents difficult to remove are not introduced, and the safety of the product is guaranteed.

The invention belongs to the technical field of biology, and particularly relates to a denitrifying hyphomicrobium high-producing strain for producing pyrroloquinoline quinone (PQQ) and application of the denitrifying hyphomicrobium high-producing strain. In particular, the present invention provides an isolated denitrifying hyphomicrobium strain comprising a 16S rDNA sequence having at least 96% identity with one or more of SEQ ID NO: 1. The invention further provides a separated denitrifying hyphomicrobium strain, and the separated denitrifying hyphomicrobium strain comprises the denitrifying hyphomicrobium strain SHYZPQQ001 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 20252085, namely the denitrifying hyphomicrobium strain SHYZPQQ001.

The invention discloses a sperm composite lubricant and a preparation method thereof, and the method comprises the following steps: mixing a polymer natural humectant, degradable carrier gel and pyrroloquinoline quinone precursor liquid according to a preset proportion to obtain a pyrroloquinoline quinone-pre-embedded moisturizing gel matrix; the preparation method comprises the following steps: dissolving a lipid membrane stabilizer in an ethanol-water mixed solution for ultrasonic emulsification, dropwise adding the emulsified lipid membrane stabilizer into a pyrroloquinoline quinone-pre-embedded moisturizing gel matrix, and stirring to obtain lipid fusion type gel; suspending a sperm motility promoter microcapsule and a plant extract in a buffer solution to form a mixed suspension, adding the mixed suspension into the lipid fusion type gel to obtain active chimeric gel, dropwise adding an antioxidant into the active chimeric gel, and stirring to obtain a microcapsule-gel dual controlled release lubricant precursor; and filtering and sterilizing the microcapsule-gel double controlled release lubricant precursor to obtain the sperm composite lubricant. The sperm motility and survival rate can be improved.

The present invention provides a new NK cell culture medium, which belongs to the field of cell culture technology. It includes basal culture medium, serum, cytokines, antibiotics and auxiliary factors, wherein the basal culture medium is selected from RPMI-1640, DMEM or IMDM, the serum is fetal bovine serum or human serum, the cytokines include IL-2, IL-7, IL-15, IL-3, VEGF, GDNF, and the antibiotics are penicillin or streptomycin. The present invention introduces auxiliary factors containing paclitaxel, cocin, ethanolamine, hyaluronic acid, sodium selenite, L-ascorbic acid, pyrroloquinoline quinone, dihydroxypropylphylline, reduced glutathione, madecassoside, D-pantothenate calcium, and estradiol into the basal culture medium, which can significantly increase the proliferation multiples and cell viability of NK cells, and at the same time, promote the expression of CD56 and CD16 by progeny NK cells. Experimental results show that after a 16-day culture period, the present invention causes NK cells to proliferate 377.6 times, the cell viability reaches 98.2%, and the expression of CD16 + CD56 + The percentage of cell number reached 83.28%.

The invention provides a PQQ-loaded porous ceramic scaffold as well as a preparation method and application thereof, and belongs to the technical field of medical materials. The preparation method comprises the following steps: dispersing pyrroloquinoline quinone powder in a phosphate buffer solution to obtain a pyrroloquinoline quinone solution, putting a zinc-doped calcium phosphate porous ceramic scaffold into the pyrroloquinoline quinone solution, shaking, and drying to obtain the PQQ-loaded porous ceramic scaffold. Experimental verification shows that the zinc-doped stent can remarkably promote regeneration and repair of a bone defect area, and the bone repair efficiency of the stent can be further enhanced through the synergistic effect by introducing PQQ.

The invention relates to the technical field of detergents, and discloses an antibacterial and antioxidant PQQ silver ion laundry detergent and a process thereof, and the antibacterial and antioxidant PQQ silver ion laundry detergent comprises the following components: 0.05-0.15 part of pyrroloquinoline quinone; the silver ion concentration of the silver ion solution ranges from 1000 ppm to 2000 ppm, and the pH value of the silver ion solution ranges from 5.5 to 6.5; 5 to 10 parts of cocamidopropyl betaine; 3 to 7 parts of fatty alcohol-polyoxyethylene ether; 6 to 12 parts of fatty alcohol polyoxyethylene ether sodium sulfate; 2-5 parts of coconut oil fatty acid ethanol amide; 1-4 parts of a composite softener, wherein the composite softener is a compound containing a cationic polymer; and 0.5-3 parts of composite plant essential oil, wherein the composite plant essential oil comprises lavender essential oil and tea tree essential oil or rosemary essential oil. By introducing the PQQ, the stabilized silver ions, the titanium dioxide and the multifunctional surface activity system, the comprehensive washing effects of strong cleaning power, lasting antibacterial property, excellent oxidation resistance and soft and protective fabric are realized.

A composition that exerts aging-delaying and anti-inflammatory effects by improving energy metabolism and mitochondrial function, a preparation method therefor, and use thereof. The composition comprises the following starting materials in parts by weight: 1-100 parts by weight of ergothioneine, 1-100 parts by weight of pyrroloquinoline quinone (PQQ) or a derivative thereof, and 20-1000 parts by weight of component C. The component C is β-nicotinamide mononucleotide (NMN), or nicotinamide riboside (NR) or a derivative thereof. Research has found that the combination of ergothioneine, PQQ or the derivative thereof, and NR or the derivative thereof or NMN can achieve a synergistic effect, with the anti-aging and anti-inflammatory effects being more significant than those of ergothioneine, PQQ or the derivative thereof, NR or the derivative thereof, or NMN alone.

A ginseng endophyte with high PQQ production, characterized in that: the taxonomic name of the ginseng endophyte is Rhodobacter methylophilus Methylorubrum sp.) W7N-70, which is deposited in the China Center for Type Culture Collection (CCTCC), with the deposit address being No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the deposit number being CCTCC M 20241885, and the deposit date being September 2, 2024. The ginseng endophyte W7N-70 obtained in the present invention has the ability to produce PQQ, and the detected PQQ synthesis amount in its supernatant reaches 75.81 mg / L, which can be effectively applied to the fermentation engineering for synthesizing PQQ. In addition, this strain can produce antibacterial metabolites and can effectively inhibit the growth of various pathogenic bacteria. This strain has excellent effects when applied to promoting plant growth and antibacterial, as well as synthesizing PQQ.

The application discloses application of Paenibacillus polymyxa in improving yield of pyrroloquinoline quinone (PQQ), and application of a living silk microorganism in improving nitrogen fixation capacity of Paenibacillus polymyxa. The application also discloses a co-cultivation system with high PQQ yield. The application further discloses the living silk microorganism with a preservation number of CCTCC NO: M 20241604. The application further discloses Paenibacillus polymyxa with a preservation number of CCTCC NO: M 20241603. The application further discloses a fermentation liquor prepared by using the co-cultivation system. The application further discloses application of the co-cultivation system and the fermentation liquor. The application can promote yield of PQQ produced by the living silk microorganism and improve nitrogenase activity of Paenibacillus polymyxa simultaneously in a bidirectional way by co-cultivating and fermenting the living silk microorganism and Paenibacillus polymyxa, and the co-cultivation fermentation liquor can improve crop nitrogen fixation, stress resistance, growth and yield.

This invention discloses a novel use of pyrroloquinoline quinone (PQQ) in improving conception rates after artificial insemination in beef cows, as well as compositions containing rumen-protected PQQ and their application methods, belonging to the field of animal nutrition and reproductive technology. Addressing the problem of low conception rates in beef cattle after artificial insemination due to early embryonic oxidative stress death, this invention proposes continuously adding an effective dose of rumen-protected PQQ to the cow's diet starting from the day of artificial insemination. This method significantly increases the conception rate of cows from approximately 40.00% in the control group to 80.00%. This invention is safe, effective, and easily applicable in large-scale farming.

The invention discloses a chemical small molecule compound and method for promoting mesenchymal stem cell mitochondria to be delivered to muscle cells. The chemical small molecule compound is composed of metformin, beta-nicotinamide mononucleotide, nicotinamide, ergothioneine and pyrroloquinoline quinone. The concentration range of each component is respectively as follows: 1 to 5mM of metformin, 0.1 to 1mM of beta-nicotinamide mononucleotide, 0.1 to 1mM of nicotinamide, 0.1 to 1mM of ergothioneine and 0.01 to 0.1 mu M of pyrroloquinoline quinone. The compound regulates and controls the mitochondrial function of mesenchymal stem cells through a staged treatment strategy: in the first stage (0-24 h), an AMPK signal channel is activated through metformin, the NAD + level is increased in combination with beta-nicotinamide mononucleotide and nicotinamide, and mitochondrial biogenesis is started; the pyrroloquinoline quinone is combined to promote DNA replication and protein synthesis of mitochondria, further increase the number of mitochondria and optimize functions.

The invention provides an anti-aging composition rich in PQQ and resveratrol and capable of activating a longevity gene. The anti-aging composition comprises the following components in parts by mass: 5-50 parts of pyrroloquinoline quinone which is fermented and purified by microorganisms; 50 to 300 parts by mass of nano resveratrol; 50 to 500 parts by mass of coenzyme Q10 embedded by beta-cyclodextrin; 50-600 parts by mass of a taurine zinc complex; 40-150 parts by mass of an acanthopanax senticosus polysaccharide extract; 20-100 parts by mass of agrocybe cylindracea polysaccharide; 10 to 50 parts by mass of lycopene; 0.1-2 parts by mass of folic acid; and 50-500 parts by mass of betaine. According to the application, core components such as pyrroloquinoline quinone, resveratrol and coenzyme Q10 are integrated, and are matched with the taurine zinc complex and various plant active polysaccharides, so that a multi-target and multi-channel synergistic anti-aging system is constructed, and optimized microbial fermentation and nanocrystallization embedding processes are adopted, so that the purity and stability of key components are greatly improved.

The invention discloses an immunity-enhancing and anti-aging tea oil health-care capsule and a preparation method thereof, and belongs to the technical field of food processing. The capsule comprises a capsule shell and a capsule content, and the capsule content comprises the following components in parts by weight: 20-40 parts of camellia oil, 12-20 parts of propolis, 8-10 parts of lucid ganoderma, 8-10 parts of American ginseng, 7-9 parts of Chinese wolfberry, 7-9 parts of Chinese yam, 5-7 parts of pyrroloquinoline quinone, 5-7 parts of yeast, 5-7 parts of vitamin E, 4-6 parts of collagen peptide and 4-6 parts of a grape seed extract. According to the capsule, through the design of the formula of the contents, all the components cooperate with one another, the good immunity enhancing and anti-aging effects are achieved, the bioavailability of key components is improved, and the production cost is reduced.

The invention belongs to the technical field of biological medicine, and particularly relates to a method for preparing pyrroloquinoline quinone and application. The invention provides a method for preparing pyrroloquinoline quinine, a seed culture medium is designed according to the requirements of microbial growth, microelements in the seed culture medium promote thallus growth, and a good thallus foundation is laid for subsequent synthesis of pyrroloquinoline quinine (PQQ); in a fermentation medium stage, namely a fermentation culture stage, PQQ synthesis enters a key stage, and trace elements beneficial to improving PQQ synthetase activity are pertinently supplemented, so that mass synthesis of PQQ is promoted. According to the method, the specific seed culture medium and the fermentation culture medium are utilized, trace elements are supplemented in the middle stage of fermentation, accurate regulation and control of the PQQ fermentation process are achieved, and the fermentation titer and purity of pyrroloquinoline quinone are improved.