618 results about "Cell number" patented technology

A method and device are provided for counting cells in a sample of living tissue, such as an embryo. The method involves obtaining a microscopic image of the unstained tissue that reveals cell boundaries, such as a differential interference contrast (DIC) image, and an optical quadrature microscopy (OQM) image which is used to prepare an image of optical path length deviation (OPD) across the cell cluster. The boundaries of individual cells in the cell cluster are modeled as ellipses and used, together with the maximum optical path length deviation of a cell, to calculate ellipsoidal model cells that are subtracted from the OPD image. The process is repeated until the OPD image is depleted of phase signal attributable to cells of the cell cluster, and the cell count is obtained from the number of cells subtracted. The method is capable of accurately and non-invasively counting the number of cells in a living embryo at the 2-30 cell stage, and can be employed to assess the developmental stage and health of human embryos for fertility treatments.

The present invention is directed to methods of monitoring cancer stem cells in patients undergoing cancer therapy to determine whether the cancer therapy is an effective cancer therapy. The present invention relates to methods for monitoring the amount of cancer stem cells prior to, during, and / or following cancer treatment of a patient. In particular, the methods provide measuring the amount of cancer stem cells i) in a sample obtained from a patient and / or ii) in a patient via in vivo imaging, e.g. at different time points before, during or after a treatment regimen for cancer. The change in amount of cancer stem cells over time allows the physician to judge the effectiveness of the treatment regimen and then to decide to continue, alter, or halt the treatment regimen if need be. The present invention also provides kits for monitoring cancer stem cells prior to, during, and / or following cancer treatment of a patient. The present invention also provides for a method of treatment of cancer, wherein such method involves the use of a therapeutic agent that stabilizes or reduces the amount of cancer stem cells in or from a patient.

A method for the determination of the red blood cell indices including the volume, and hemoglobin content and concentration for individual red blood cells, as well as red blood cell population statistics, including total number of red blood cells present in the sample, and mean values for each of the aforementioned indices within a substantially undiluted blood sample is provided.

A method for discovering neurogenic drugs is revealed. The method allows for systematic screening of test agents such as libraries of compounds. The method consists of exposing test agents to cultures of differentiating neural progenitor cells and measuring their effects on increasing the overall cell number and / or the number of neurons.

A cell reselection method and system using an active measurement set in a mobile communication, which determines whether the user equipment enters in a power-saving mode based on the signal strength and strength variability of a serving cell and the mobile feature of a user equipment. In the power-saving mode, in accordance with the signal strength of neighboring cells on the serving cell, an active measurement set is formed with cells that are selected from the neighboring cells broadcasted by a WCDMA system and have stronger signals. The user equipment only requires measuring the cells in the active measurement set. Thus, since the cell number of the active measurement set is smaller than that defined by the WCDMA system, the number of neighboring cells required to be measured is reduced, so as to reduce the power dissipation and increase the idle time on the user equipment.

One or more cells can be cultured when confined in space by barriers. The distance between barriers can be comparable to the size of a cell to be cultured. The space between barriers can also be sufficiently small to allow control of cell properties or monitoring of the cell(s) cultured therein. The cell(s) may be confined completely or may be mobile between two opposing barrier surfaces. The gap between two opposing barriers may be sufficiently narrow to allow only a monolayer of cells to be cultured. A barrier can be transparent. The surfaces of the barriers may have one or more pre-selected characteristics that mimic the characteristics of a biological niche of the cells(s). The number of cells in a cell culture may be limited to permit control of properties of individual cells. The cultured cell(s) may be monitored, such as imaged, over a long period of time, using standard bright field or fluorescent imaging techniques.

The invention relates to diagnostic methods for assessing the need of a subject for treatment with an anti-oxidant, or alternatively, for determining the utilization efficiency and ultimate effectiveness of anti-oxidant therapy in subjects having been treated with antioxidants. More specifically, the methods of the present invention are particularly useful in prophylactic assessment of individuals at risk for developing diseases or conditions in which oxidative stress plays a role, such that an appropriate therapeutic regimen can be prescribed for that individual, thus leading to alternative therapies and / or life style changes. The invention further relates to methods for assessing the need for, the utilization efficiency and the effectiveness of therapy in subjects having received therapy with specific antioxidant and immune enhancing formulations. Kits are also provided for measuring the levels of markers of oxidative stress and immune cell numbers.

A method and device are provided for counting cells in a sample of living tissue, such as an embryo. The method involves obtaining a microscopic image of the unstained tissue that reveals cell boundaries, such as a differential interference contrast (DIC) image, and an optical quadrature microscopy (OQM) image which is used to prepare an image of optical path length deviation (OPD) across the cell cluster. The boundaries of individual cells in the cell cluster are modeled as ellipses and used, together with the maximum optical path length deviation of a cell, to calculate ellipsoidal model cells that are subtracted from the OPD image. The process is repeated until the OPD image is depleted of phase signal attributable to cells of the cell cluster, and the cell count is obtained from the number of cells subtracted. The method is capable of accurately and non-invasively counting the number of cells in a living embryo at the 2-30 cell stage, and can be employed to assess the developmental stage and health of human embryos for fertility treatments.

An electrodeionization, (EDI) apparatus has flow cells with a sparse distribution of ion exchange (IX) material or beads. The beads extend between membranes defining opposed walls of the cell to separate and support the membranes, and form a layer substantially free of bead-to-bead dead-end reverse junctions. The beads enhance capture of ions from surrounding fluid in dilute cells, and do not throw salt when operating current is increased. In concentrating cells, the sparse bead filling provides a stable low impedance bridge to enhanced power utilization in the stack. A monotype sparse filling may be used in concentrate cells, while mixed, layered, striped, graded or other beads may be employed in dilute cells. Ion conduction paths are no more than a few grains long and the lower packing density permits effective fluid flow. A flow cell thickness may be below one millimeter, and the beads may be discretely spaced, form a mixed or patterned monolayer, or form an ordered bilayer, and a mesh having a lattice spacing comparable to or of the same order of magnitude as resin grain size, may provide a distributed open support that assures a stable distribution of the sparse filling, and over time maintains the initial balance of uniform conductivity and good through-flow. The cells or low thickness and this resin layers relax stack size and power supply constraints, while providing treatment efficiencies and process stability. Reduced ion migration distances enhance the ion removal rate without reducing the product flow rate. The sparse resin bed may be layered, graded along the length of the path, striped or otherwise patterned. Inter-grain ion hopping is reduced or eliminated, thus avoiding the occurrence of salt-throwing which occurs at reverse bead junctions of prior art constructions. Conductivity of concentrate cells is increased, permitting more compact device construction, allowing increases in stack cell number, and providing more efficient electrical operation without ion additions. Finally, ion storage within beads is greatly reduces, eliminating the potential for contamination during reversal operation. Various methods of forming sparse beds and assembling the stacks are disclosed.

The invention discloses a method for simultaneous and efficient amplification of CD<3+>CD<56+>CIK cells and CD<3->CD<56+>NK cells. The method comprises the steps as follows: the concentration of separated PBMC (peripheral blood mononuclear cells) is adjusted by a serum-free medium containing autologous plasma, an Anti-CD16 antibody, IL-2 and IL-15 are added, and then the mixture is transferred into a T175 culture flask for culture; an Anti-CD3 antibody and an Anti-CD137 antibody are added; a serum-free medium containing the autologous plasma, IL-2 and IL-15 is supplemented every two days according to the cell growth condition; the cell concentration is controlled to be about 1.5*10<6> / ml; and after culture is performed for 14-21 days, large quantities of high-purity CD<3+>CD<56+>CIK cells and CD<3->CD<56+>NK cells can be obtained simultaneously, and the total cell quantity can reach an effective value of the cell quantity required for adoptive cellular immunotherapy clinically for tumor. The method for simultaneous and efficient amplification of the CD<3+>CD<56+>CIK cells and the CD<3->CD<56+>NK cells is simple, convenient, effective and high in cell killing activity.

The invention provides a buoy for algae monitoring and early warning in a drinking water source area, which comprises a sampling tube for collecting water samples, a lifting motor for controlling the sampling tube to lift, a peristaltic pump for controlling the sampling tube to collect the water samples, an algae monitoring instrument for monitoring total algae and cell number of microcystis population in the water samples and figuring out the cell density, a data acquisition device for collecting monitored and / or calculated data, a wireless communication module for sending the data to a monitoring center, a solar panel for collecting solar energy and converting the solar energy to electric energy, a battery pack for storing the electric energy for later use, a photovoltaic controller for stabilizing a voltage and charging the battery pack, and a power panel for providing power supply for all power utilization modules. The buoy can perform monitoring by measuring the cell number of the algae, be free of interference of CDOM (colored dissolved organic matter) fluorescence, be suitable for the large concentration range of 103-1010 cells / L, be suitable for water bodies in various different algae cell concentrations and different seasons and greatly improve the accuracy and the applicability in algae monitoring in the drinking water source area.

A computerized system for calling a plurality of cell phones in compliance with the TCPA, comprises a call center database; call center workstations operated by transfer agents; client workstations operated by client associates; and a computer processor that retrieves a cell phone number of a consumer to call, in response to an initiate call instruction from a respective transfer agent, dials the cell phone number, connects the call to the respective transfer agent if a live voice is detected; receives a transfer call request from the respective transfer agent, transfers the call with the one consumer to a client associate, using the cell number as the origination number of the call to identify the one consumer to the client associate, disconnects the connection between the one consumer and the respective transfer agent, and identifies a next consumer to call upon request by the transfer agent.

The present invention includes a method of utilizing four peptide hormones to inhibit the growth of cancer(s). A dramatic decrease in the number of human pancreatic adenocarcinoma cells (i.e., the type of cancer with the highest mortality, with patients only surviving four months) was observed responsive to treatment. The application of the invention would be to utilize one or more of these peptide hormones alone and / or in combination to treat cancer. The ability of these peptide hormones to decrease the number of adenocarcinoma cells has implications for adenocarcinomas at other sites in the body with the majority of cancers of the breast, colon and prostate also being adenocarcinomas. Adenocarcinomas also occur in the lung and other tissues. Treatment of a wide variety of cancers in addition to adenocarcinomas is anticipated by the present invention.

The invention discloses a method for performing genome amplification by using embryo blastula-stage cells, performing chromosome detection on the preimplantational embryo by combining a high-flux sequencing technique and screening out the chromosome normal embryo. The method can comprehensively and completely analyze the genetic variation information of the embryo genome, thereby instructing the preimplantational embryo selection, reducing the hereditary diseases and enhancing the success rate of test tube babies. The method comprises the following steps: blastula-stage trophocyte separation; genome amplification; DNA (deoxyribonucleic acid) segmentation; and Proton library establishment, mounting sequencing and sequencing data analysis. By using the blastula-stage embryo to perform trophocyte separation detection, the method avoids the injuries of cleavage-stage cell separation to the embryo, obtains higher cell quantity than the cleavage stage, and enhances the success rate and amplification effect of genome amplification. After the blastula-stage embryos are subjected to the natural elimination process, the high-quality blastula-stage embryo is selected for detection, thereby saving the cost.

The invention provides an image processing method and device. The method includes: acquiring a target image including algal cells; binarizing the target image to obtain a target gray image; extracting connected areas in the target gray image, and calculating area of each connected area; dividing preset algal area by the area of each connected area to obtain a quotient of each connected area; using a sum of the quotients of the connected areas, as the amount of the algal cells included in the target image. The algal cells intersecting together are not subjected to image splitting, thus, the number of the algal cells can be accurately counted, and accuracy of counting the algal cells is improved.

The invention discloses a complete medium and a human amnion-derived mesenchymal stem cell (hAMSCs) culture method. The complete medium is prepared by adding 3 to 10 percent of autologous umbilical cord blood serum into low-sugar Dulbecco minimum essential medium solution according to a volume ratio. The culture method comprises: (1) separation; (2) primary culture; and (3) subculture. The methodusing the complete medium in the hAMSCs culture has the advantages that: the risk of using fetal calf serum is avoided; although the need of adding L-glutamine, non-essential amino acid, 2-mercapitoethanol, pyruvic acid and the like is obviated, the high proliferation properties and phenotypic characteristics of the hAMSCs and expression of multilineage differentiation marker genes sand proteins of some stem cells can still be retained; and in subculture, the wall adherence fastness of the hAMSCs is much lower than that in fetal bovine serum (FBS) culture, the digestion time is reduced obviously, and the damage of trypsinization to cells and loss of cells are reduced.

The invention discloses a quantitative optical molecular tomographic device and a quantitative reconstruction method. The molecular tomographic device comprises a bioluminenscent tomographic data acquisition platform, a Micro CT (Computerized Tomography) system, a quantitative calibration system and a quantitative reconstruction computer, wherein the bioluminenscent tomographic data acquisition platform is used for capturing the distribution condition of bioluminescent light sources emerging on the body surface of a small animal, and the Micro CT system is used for acquiring the anatomical structure information of the small animal body. The quantitative reconstruction method comprises a quantitative method of a receiving light source based on a field of view and a reconstruction method based on a finite element subdivision network. By utilizing the quantitative optical molecular tomographic device and the quantitative reconstruction method, three-dimensional light source distribution and the quantitative information of a cell number in a small animal body can be inverted through the two-dimensional light source distribution and the quantitative energy information on the surface of the small animal body.

The invention discloses an isolated culture method of human adipose-derived stem cells and a construction method of a stem cell bank. The isolated culture method comprises the following steps of: (1) collecting a human adipose tissue; (2) obtaining and separating the human adipose-derived stem cells; (3) culturing the stem cells; (4) detecting and cryopreserving; and (5) constructing the stem cell bank. According to the invention, the mixed collagenase prepared from D-Hanks balanced salt solution and containing type I and type VI collagenases is employed to digest the adipose tissue so that the tissue is digested more thoroughly, the number of parenchyma cells is obviously reduced and tissue blocks are removed; the MCDB-201 culture solution containing 10-15% of fetal calf serum and 10<3>-10<5>U / Ml LIF (Leukemia Inhibitory Factor) is used for culturing the isolated stem cells so that the cells proliferate quickly and are good in morphology; the differentiation of the stem cells can be effectively inhibited and the characteristics of the primary stem cells are ensured; and in the meantime, the human adipose-derived stem cells can be promoted to grow for a long time, and to keep the features such as self-renewal and multipotential differentiation; and moreover, the obtained stem cells are saved in the bank constructed so that better-quality seed cell source is guaranteed.

The invention discloses antibacterial aluminum and a manufacturing method thereof. The manufacturing method comprises the following steps: (1) a pretreated aluminum alloy serves as an anode to form two electrodes with a cathode module, and the anodic oxidation is performed in electrolyte to prepare a porous aluminum oxide film on the surface of the aluminum alloy, wherein the hole way unit cell number on the unit area of the porous aluminum oxide film is 70-100*109 / cm2; and the hole way parameters in unit cells are 1-100 microns of the hole depth and 10-50 microns of the aperture; and (2) the aluminum alloy with the porous aluminum oxide film obtained in the step (1) and an electrode module form two electrodes; antibacterial metal is electrolyzed and deposited in hole ways of the porous aluminum oxide film by using deposition liquid; and the particle size of the deposited antibacterial metal is 1-100 nanometers. The manufacturing method of the antibacterial aluminum is simple in process, convenient in operation and low in manufacturing cost; the antibacterial metal is uniformly and stably deposited; the bacterial resisting and killing effect of the aluminum alloy is long in lasting time; and the bacterial resisting and killing rate is high up to reach 99.99%. Dissoluble precipitations cannot be separated out even if long-time use of electrolysis and deposition liquid.

Aspects of the present disclosure include methods for processing a biological sample. Methods according to certain embodiments include contacting a biological sample having cells with an assay reagent that includes one or more analyte-specific binding members to produce a biological sample assay composition and introducing the biological assay composition into an inlet of a flow cytometer having an integrated acoustic separator. Systems, including a flow cytometer with integrated acoustic separator having one or more acoustic concentrator devices suitable for practicing the subject methods are also described.

The present invention generally provides therapeutic compositions and methods for treating a disease, disorder, or injury characterized by a deficiency in cell number. The method involves inducing a heat shock response in tissue or organ effected by disease and recruiting stem cells to repair or regenerate the disease-effected tissue.

The present invention relates to kits and methods for performing dual-staining immunohistochemistry (IHC) for the detection of specific cell populations in tissue samples containing heterogeneous populations of cells, which can be observed by a light microscope for co-localization of distinct pigments. The method includes providing a tissue sample comprising fixed cells; exposing the sample to first and second ligands that recognize different marker proteins found at the same cellular location, thereby forming a ligand-labeled sample; exposing the ligand-labeled sample to first and second labeling reagents, the first labeling reagent binding to the first ligand and the second labeling reagent binding to the second ligand, the first and second labeling reagents each forming distinct pigments; and identifying the number of cells that display only one particular pigment, or more than one pigment, by the different coloration of the cellular location labeled by the distinct pigment.

The invention relates to a device and method for noninvasive continuous monitoring of quantity or concentration of dynamic cells, in particular to a device and method for noninvasive continuous on-line or off-line monitoring of quantity or concentration of dynamic cells in adherent cell cultures (usually as stem cell cultures or other therapeutic cell cultures) or cell and tissue cultures. The device comprises: a cell culture medium supply system, one / one type of or multiple / multiple types of upstream biomedical indicator detection head(s) or connector(s), a cell culture , one / one type of or multiple / multiple types of downstream biomedical indicator detection head(s) or connector(s), one or multiple speed controllable sterile driving unit(s) of fluid, a waste or harvested liquor system, liquid conveying pipeline systems that are driven and controlled in speed by the speed controllable sterile driving unit(s) of fluid for connecting the above components in order. And the upstream and downstream biomedical indicator detection head(s) or connector(s) are connected to a monitoring and controlling system and / or a computer so as to obtain, process and monitor data, and furthermore provide feedbacks for the whole cell culture system through a signal feedback system for realizing control.

A method is provided for use with extracted blood, including (a) applying blood to a first gradient suitable for selecting first-pass cells having a density less than 1.077 g / ml; (b) applying the first-pass cells to a second gradient suitable for selecting second-pass cells having a density between 1.055 and 1.074 g / ml; (c) increasing the number of cells having a density between 1.055 and 1.074 g / ml, by culturing the second-pass cells for a period lasting between 3 and 30 days; and (d) identifying endothelial progenitor cells in the cultured cells. Other embodiments are also described.

A functionalized tip is incorporated into catheters for the cytometric delivery of cells into the brain and other body parts. For use in the brain, the tip forms part of a neurosurgical probe having a proximal end and a distal end. In addition to the functionalized tip, the probe has at least one cell slurry delivery lumen and a plurality of optical fibers configured along the probe, terminating in the tip to provide the photo-optical capability needed to monitor the viability and physiological behavior of the grafted cells as well as certain characteristics of the cellular environment. Details are also presented of the use of a neurocatheter having a cytometric tip of the type disclosed in the invention, as employed within the context of a feedback and control system for regulating the number of cells delivered to the brain of a patient.

The invention discloses a multi-cell automatic tracking method and system based on a plurality of task ant systems. The plurality of task ant systems work independently and cooperatively, each independent task ant system module corresponds to a cell for tracking, and a corresponding variable describes the task completion probability; for each independent task ant system, and the ant individual state is firstly determinate by ant model probability and likelihood function values and then performed with state local optimizing through a local adjusting module; for an ant system cooperating layer, on the basis than an effective likelihood function is defined, an optimum histogram template in the current plurality of ant colony systems is found out through a mutually information exchanging method to update a template histogram image base, further model probability, weight and influence area of each ant and task finishing probability of each ant system are further updated, and cell state estimation is given out finally. The multi-cell automatic tracking method and system based on the plurality of task ant systems can achieve various multi-cell automatic tracking with different dynamic characteristic parameters, moving in close range and with cell number time-varying.

The invention discloses a method for improving the biomass and oil fat yield of microorganisms. The method comprises the following steps: (1) carrying out activated culture: respectively inoculating chlorella pyrenoidosa and rhodotorula glutinis cells into a culture medium, carrying out activated culture to a logarithmic phase, and taking a product as a seed solution; (2) respectively inoculating chlorella pyrenoidosa and rhodotorula glutinis into a shake flask according to different proportions, wherein each shake flask is internally filled with a culture medium, putting the shake flasks into a lighting shaker, and culturing; (3) respectively inoculating chlorella pyrenoidosa and rhodotorula glutinis cells into a BG11 culture medium, wherein the ratio of chlorella pyrenoidosa cell number to the rhodotorula glutinis cell number is equal to 3: 1, and the BG11 culture mediums contain 1.6g / L of yeast extract and have different glucose concentrations; putting the inoculated shake flasks into the lighting shaker, and culturing for 12 days; (4) after the culture is finished, centrifuging microbial cells, washing and carrying out freeze drying to obtain powder; the powder can be used for measuring and evaluating the dry weight concentration, total lipid content, fatty acid content and yield of the biomass. The method has the advantages that the biomass and oil fat yield of the oil-containing microorganisms are remarkably improved by utilizing a mixed culturing mode, and are 3-4 times or more than those of microorganisms cultured under a single culture condition; the method is relatively simple and convenient, and the energy consumption and the production cost are effectively reduced.

A seating detector comprises a plurality of cells provided at a seating surface to be defined by rows and columns for detecting partial loads, an unevenness calculating means for calculating the number of cells when a partial pressure detected at the each cell is larger than a partial pressure detected at one abutting cell positioned next to the cell at one side thereof and a partial pressure detected at the other abutting cell positioned next to the cell at the other side thereof, or when a partial pressure detected at the each cell is smaller than a partial pressure detected at one abutting cell positioned next to the cell at one side thereof and a partial pressure detected at the other abutting cell positioned next to the cell at the other side thereof, and a determining means for determining that the seat is occupied by a child restraint system based on a comparison between the number of the cells and a threshold.

A database system that accommodates any type of phenomenon or thing, with a simple table structure and an extremely small-scale program. Category identification information of the content to be registered, item names that represent an attribute and / or a function included in the category, and attributes of data representing the substantial contents of these items (item data attributes) are registered in each data cell of a data property table (21) in units of rows, and category identification information, title names, and substantial contents associated with each item are registered in data cells of a main data table (22) in units of rows. The item names of the table (21) and the substantial contents of the table (22) correspond by means of cell numbers, and when a database management system (10) performs data input, storage, searching, and output, the various methods are generated with row-direction data of the table (21) as a message.