87 results about "Salidroside" patented technology

The invention provides application of acidophilous glycosyltransferase in salidroside production, and belongs to the technical field of biological engineering. The problem of producing salidroside under the acidic condition is solved. Comprising an application of acidophilous glycosyl transferase with an amino acid sequence as shown in SEQ ID NO.1 in salidroside production under an acidic condition and an acidophilous escherichia coli engineering strain for producing salidroside. The escherichia coli engineering strain overexpresses a mutant 3-deoxy-D-arabinoheptulose-7-phosphate (DAHP) synthetase gene aroGfbr, a cyclohexadiene dehydrogenase gene tyrC, a glucose phosphate mutant enzyme gene pgm and a UDP-glucose pyrophosphorylase galU, overexpresses a phenylpyruvate decarboxylase gene ARO10 derived from saccharomyces cerevisiae, and can be used for producing a mutant 3-deoxy-D-arabinoheptulose-7-phosphate mutant enzyme. The kit comprises an ethanol dehydrogenase gene ADH6 and a glycosyl transferase gene LrUGT85AF8. The method is mainly used for producing salidroside under an acidic condition.

According to the present invention, glycosyl transferase UGTBL1 derived from Bacillus licheniformis ZSP01 is adopted as a research object, AlphaFold 2 is adopted to carry out modeling, molecular docking, molecular dynamics simulation and other technologies, amino acids in an active pocket region of glycosyl transferase are analyzed, site-directed mutagenesis is performed on the amino acids to obtain a series of glycosyl transferase mutants, and salidroside can be highly selectively catalyzed and synthesized.

The invention discloses a method for detecting the content of salidroside in a rhodiola rosea extracting solution based on artificial intelligence. The method comprises the following steps: S1, performing spectral scanning of different wavebands on an extracting solution sample in a plurality of near-infrared wavelength ranges; s2, performing multi-dimensional interference suppression processing and signal purification; s3, carrying out spectral feature compression calculation to obtain a salidroside feature intensity factor; s4, carrying out salidroside characteristic signal-to-noise ratio enhancement and purity index calculation; s5, predicting the content of salidroside and outputting a content prediction score; and S6, mapping the content prediction score into the final salidroside content. According to the method, spectral signal purification, nonlinear feature extraction, dynamic noise enhancement, artificial intelligence prediction modeling and a secondary recheck mechanism are utilized to realize adaptive analysis of extracting solutions from different sources, so that the detection accuracy and stability are remarkably improved, and rapid and traceable salidroside content detection can be realized under the condition that a large precise instrument is not needed.

The present invention provides an anti-aging, whitening, freckle-removing, moisturizing skin care water and a preparation process thereof. A preparation process for an anti-aging, whitening, freckle-removing, moisturizing skin care water comprises the following steps: supercritical CO2 extraction of oat-yarrow extract; ultrasonic-enzymatic hydrolysis-assisted reflux extraction and concentration; adding oxidized resveratrol, salidroside and ellagic acid; adding purified water to dissolve and adding sodium alginate and glycerol; adding p-anisic acid and aromatic alcohol and homogenizing and mixing. The present invention is a skin care water prepared by first extracting extracts of bletilla striata, wormwood leaf, peony bark, oat and yarrow, and then adding oxidized resveratrol, salidroside, ellagic acid, sodium alginate, glycerol, p-anisic acid and aromatic alcohol. The skin care water not only has the effects of anti-aging, whitening, freckle removal and moisturizing, but also because its raw materials are all natural plant ingredients and no chemical agents are added, therefore, it will not irritate the skin and has no side effects, and can be used for a long time.

The invention discloses recombinant escherichia coli capable of producing salidroside and application, exogenous genes are introduced by utilizing a CRISPR / Cas9 technology, the recombinant escherichia coli capable of improving the fermentation yield of the salidroside is obtained by modifying and screening key genes, and finally, the salidroside is obtained by optimizing a fermentation tank process. The recombinant Escherichia coli can reach the highest yield of salidroside produced by fermentation of the scale at present on a 5L fermentation tank. The salidroside is fermented in a 5L fermentation tank for 104 hours, so that the maximum accumulation amount of the salidroside is 33.68 g / L, and industrial enlarged production is facilitated.

The present application provides a rhodiolide composition with a core-shell-shell structure, a preparation method thereof comprising the steps of: 1) EGCG inclusion: adding beta-cyclodextrin and EGCG into water, ultrasonic, forming an inclusion compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, dissolving in chloroform to form a uniform solution; weighing rhodiolide, dissolving in the uniform solution, rotary evaporation to remove chloroform, then adding the inclusion compound and PBS buffer into the container, hydrating, forming a liposome wrapping rhodiolide and inclusion compound; 3) shell loading: weighing collagen, mixing with chitosan solution uniformly to form a complex; adding the complex into the liposome, the complex is coated on the outer layer of the liposome, centrifugal purification, obtaining a rhodiolide composition with a core-shell-shell structure. The present application belongs to the technical field of cosmetic raw materials, and the rhodiolide composition provided has the advantages of high stability, good transdermal effect, and significant anti-aging effect.

The invention provides a salidroside composition with a core-shell-shell structure, and a preparation method of the salidroside composition comprises the following steps: 1) EGCG (epigallocatechin gallate) clathration: adding beta-cyclodextrin and EGCG into water, and carrying out ultrasonic treatment to form a clathrate compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, and dissolving in chloroform to form a uniform solution; the preparation method comprises the following steps: weighing salidroside, dissolving the salidroside in a uniform solution, carrying out rotary evaporation to remove chloroform, then adding an inclusion compound and a PBS (Phosphate Buffer Solution) into a container, and hydrating to form lipidosome wrapping the salidroside and the inclusion compound; 3) shell loading: weighing collagen, and uniformly mixing the collagen with the chitosan solution to form a compound; the salidroside composition with the core-shell-shell structure is obtained by dropwise adding the compound into the lipidosome, coating the outer layer of the lipidosome with the compound and carrying out centrifugal purification. The invention belongs to the technical field of cosmetic raw materials, and provides a salidroside composition which has the advantages of high stability, good transdermal effect, remarkable anti-aging effect and the like.

The invention discloses a culture method for hypoxia culture of umbilical cord mesenchymal stem cells, which comprises the following steps: hypoxia pre-adaptive culture: inoculating umbilical cord mesenchymal stem cells in a culture medium containing bFGF (basic fibroblast growth factor), glutathione, astragalus polysaccharide and salidroside, and transferring the umbilical cord mesenchymal stem cells into a 5% O2 environment after the umbilical cord mesenchymal stem cells are preliminarily adhered to the wall in a normal oxygen environment; performing low-oxygen enrichment culture: replacing a second culture medium containing astragalus polysaccharide, salidroside, D-ribose, sodium pyruvate and D-glucose, and promoting efficient cell proliferation under 2% O2; performing function strengthening culture: adding tanshinone IIA and GSK-269962A, inducing cells to secrete VEGF under 1% O2, and maintaining high dryness. According to the method, through the synergistic effect of the three-stage gradient oxygen concentration and the specific serum-free culture medium, the cell proliferation multiple is remarkably increased, the total apoptosis rate is reduced, VEGF secretion is promoted, the multidirectional differentiation potential is reserved, the culture method is standardized in operation, the serum-free culture medium is definite in component, and a high-quality stem cell culture scheme is provided for the field of regenerative medicine.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium, which is composed of a basic culture medium and additive components, and the additive components comprise, by final concentration, 5-20 [mu] g / mL of astragalus polysaccharide, 0.5-2.0 [mu] g / mL of salidroside, 0.1-0.5 [mu] g / mL of tanshinone IIA, 0.1-0.3 mg / mL of sodium pyruvate, 5-15 mM of D-ribose, 3-6 mg / mL of recombinant human albumin, and 5-10 [mu] g / mL of recombinant transferrin; the invention also discloses a method for culturing umbilical cord mesenchymal stem cells under the hypoxia condition by using the serum-free culture medium. The method comprises the steps of cell inoculation, culture under the hypoxia condition, subculture, cell passage and the like. The astragalus polysaccharide, the salidroside, the tanshinone IIA, the sodium pyruvate, the recombinant human albumin and the recombinant transferrin are added into the serum-free culture medium, so that nutrition supply and function maintenance of cells under a low-oxygen condition are guaranteed, the proliferation rate and the differentiation capacity of the cultured cells are improved, the apoptosis rate of the cells is reduced, and the survival rate of the cells is increased. Wide application prospects are realized in the fields of cell culture, regenerative medicine and the like.

The application discloses a mouse wearable micro-injection device for slow release of rhodiolin, and relates to the technical field of medical miniaturized devices. The device comprises a fixing frame, a slow injection device arranged at the upper end of the fixing frame, and a flow guide device connected with the slow injection device. The slow injection device comprises a syringe body internally provided with a liquid storage area and a reaction area. An injection plug is arranged between the liquid storage area and the reaction area. The side edge of the injection plug is in close contact with the inner wall of the syringe body. The flow guide device is in communication with the liquid storage area. The device can realize accurate control of the drug release rate through three different reaction mechanisms, i.e. gas driving of a drug bin, physical expansion of an expansion bag and composite expansion of an expansion bin. In addition, the design of the hanging ear port in the fixing frame can not only disperse the pressure on the ears, but also reduce the fluctuation range of the mouse's corticosterone level in combination with the lightweight structure design.

The invention provides glycosyl transferase and application thereof in preparation of salidroside, and relates to the technical field of enzyme engineering. The glycosyl transferase FpUGT provided by the invention is derived from fraxinus pennsylvanica, the existence of the glycosyl transferase gene capable of directly catalytically synthesizing the salidroside in the plant is not reported in literatures, and a new biological way is provided for finding the glycosyl transferase for efficiently synthesizing the salidroside. And a new biological material is provided for preparing the salidroside.

The invention discloses a method for extracting and purifying salidroside from salidroside fermentation liquor, which comprises the following steps: (1) adding tert-butyl alcohol into the salidroside fermentation liquor, filtering, and collecting clear liquid; (2) concentrating the clear solution obtained in the step (1) under reduced pressure to obtain a concentrated solution, adding petroleum ether for extraction, and collecting a water phase; (3) adding ammonium sulfate into the water phase obtained in the step (2), uniformly mixing, adjusting the pH to be acidic, adding ethyl acetate for extraction, and collecting an organic phase; and (4) concentrating the organic phase obtained in the step (3) under reduced pressure, and drying. Through a solvent polarity gradient matching technology and a salting-out enhanced phase separation mechanism, the structural stability of the salidroside is guaranteed, meanwhile, the extraction and purification technological process is simplified, the separation efficiency is remarkably improved, the purity and yield of the salidroside obtained through extraction and purification with the method are both larger than or equal to 90%, and the method is suitable for industrial production. The extraction and purification process provided by the invention is also provided with a solvent recovery system, and the efficiency and economy of industrial production are both considered.

The invention discloses low-caffeine nerve stimulation tea wine and a preparation method thereof, the low-caffeine nerve stimulation tea wine is prepared by blending eucommia wood aged tea wine, cherry wood aged tea wine and cherry wood / Hainan dalbergia odorifera wood aged tea wine with different baking degrees, and the mass ratio of geniposidic acid to salidroside to formononetin in the wine body is (2-30): (1-15): 1. The mass ratio of the genkwanin to the alpinia japonica to the sesamol is (0.5-8): (0.15-6): 1, the mass ratio of the isoliquiritigenin to the vitexin to the syringaldehyde is 1: (0.15-5): (4-18), and the total content of the geniposidic acid, the salidroside, the formononetin, the genkwanin, the alpinia japonica, the sesamol, the isoliquiritigenin, the vitexin and the syringaldehyde in the wine is 40-250 mg / 100 mL. By combining and matching the functional components in the wood and the proportion of the functional components, the antagonistic effect on caffeine nerve stimulation in the tea wine is achieved.

The invention discloses application of a combination of scutellarin and salidroside in treatment of solar dermatitis, finds that the combination of scutellarin and salidroside has a synergistic effect on treatment of solar dermatitis for the first time, and provides a brand new and effective drug combination strategy for treatment of solar dermatitis. A new thought is provided for research and development of anti-solar dermatitis drugs, and the application value and the industrial conversion prospect are good.

The application provides a mutant of acidophilic glycosyltransferase at the 400th position and application thereof in production of salidroside, and belongs to the technical field of bioengineering. The application solves the problem of low synthesis efficiency of salidroside under acidic conditions. The application comprises an acidophilic glycosyltransferase mutant, wherein the glycosyltransferase mutant is obtained by mutating the 400th position of the amino acid shown in SEQ ID NO. 2. The mutant is mainly used for high-yield production of salidroside under acidic conditions.

The invention discloses a method for producing high-purity salidroside from fermentation liquor through green purification, and belongs to the field of salidroside. The invention aims to solve the problems of high energy consumption, high pollution and low yield of purification processes such as decoloration, extraction, crystallization and freeze-drying by adopting adsorbents such as activated carbon in the existing salidroside separation and purification technology. The method comprises the following steps: carrying out solid-liquid separation on salidroside fermentation liquor through ceramic membrane microfiltration; then carrying out ultrafiltration by using an ultrafiltration membrane; diluting with purified water to serve as a chromatography loading solution, and adsorbing with a macroporous adsorption resin column with a non-polar styrene skeleton for primary decoloration and purification; then carrying out vacuum concentration; dissolving with ethanol, directly loading to an aluminum oxide chromatographic column for secondary decoloration, and eluting with 100%-80%-50% ethanol after loading is finished; concentrating under reduced pressure, and refining through a polymer filler until the purity is greater than or equal to 99.5%; and performing vacuum concentration and spray drying. Technical support and high-quality raw materials can be provided for the industries such as medicine, cosmetics and functional food.

The present application relates to a kind of selectively improved glycosyltransferase UGTBL1 mutant and its catalytic synthesis of application of salidroside, belong to genetic engineering and enzyme engineering field.The mutant obtained by single point mutation technology includes the amino acid sequence of 132th amino mutation, the amino acid sequence of the glycosyltransferase is as shown in SEQ ID NO:1.The research results show that the glycosyltransferase mutant S132V, S132G described in the application has good selectivity, can efficiently catalyze tyrosol and uridine diphosphate glucose to synthesize salidroside, and the selectivity is 89%, suitable for the enzyme preparation of salidroside, with wide application prospect.

The invention relates to the technical field of biological medicines, in particular to an exosome for atomization intervention of a plateau pulmonary heart disease and a preparation method and application thereof, the preparation method comprises the following steps: S1, preparing a lung-targeted exosome, and purifying to obtain an azide exosome; s2, carrying out amination modification and activation on the salidroside, so as to obtain DBCO modified salidroside; s3, chemical connection is clicked, and a composite exosome is generated; and S4, purifying the composite exosome, adding a freeze-drying protective agent, uniformly mixing, freeze-drying, and atomizing the freeze-dried exosome for intervening the plateau pulmonary heart disease. Through collaborative design of molecular engineering modification and a preparation process, the exosome system for intervening the plateau pulmonary heart disease by atomization, which has lung targeting capability, a biological activity maintaining function in a plateau low-oxygen environment, structural stability in an atomization process and a freeze-drying-redissolving quick response characteristic, is constructed.

The invention discloses a staying-up glowing composition containing multiple plant extracts. The staying-up glowing composition containing the multiple plant extracts is prepared from the following components in parts by mass: 3 to 5 parts of extract A, 10 to 15 parts of extract B, 2 to 4 parts of extract C, 1.5 to 3 parts of curcumin nano-liposome, 2 to 5 parts of lactobacillus fermentation filtrate, 1 to 3 parts of water-locking magnetite, 0.1 to 0.3 part of xanthan gum / acrylate compound thickening agent and 0.5 to 1 part of preservative composition. According to the staying up glowing composition containing the multiple plant extracts, the target component of the extract A is jasmonic acid methyl ester, the target component of the extract B is salidroside and nausea acid, and the curcumin nano-liposome is compounded, so that the staying up glowing composition containing the multiple plant extracts is obtained; compared with similar products sold in the market at present, the anti-oxidation and anti-saccharification capacities of the staying-up glowing composition can be enhanced in a targeted manner, the transdermal efficiency is improved, and meanwhile, the DNA repair effect and the anti-inflammatory effect are improved.

According to the application of the salidroside in preparation of the medicine for treating the Alzheimer's disease by relieving ferroptosis, the salidroside is used for relieving the ferroptosis of human neuroblastoma (SH-SY 5Y) cells induced by A [beta] 1-42 by inhibiting a voltage dependent anion channel 1 (VDAC1) and activating an AKT / GSK-3beta pathway, and the application of the salidroside in the preparation of the medicine for treating the Alzheimer's disease by relieving the ferroptosis of the human neuroblastoma (SH-SY 5Y) is realized by inhibiting the voltage dependent anion channel 1 (VDAC1) and activating the AKT / GSK-3beta pathway. The salidroside can relieve the ferroptosis of SH-SY 5 Y cells induced by A beta 1-42 under the optimal concentration of 30 mM. After salidroside treatment, the expression quantity of GPX 4 and FTH 1 is increased, and the expression quantity of IREB 2 is reduced. The cell apoptosis rate is reduced, and the Fe < 2 + > content and ROS level in cells are also reduced. After si-VDAC 1 is transfected, the change trend of si-VDAC 1 is similar to the trend after salidroside treatment. In addition, the treatment of an AKT inhibitor and an AKT activator shows that an AKT / GSK-3beta signal channel participates in the process of inducing the ferroptosis of the SH-SY 5 Y cells by the Abeta1-42.

The application provides a salidroside derivative, a preparation method and application thereof. The structure of the salidroside derivative is shown in formula I: wherein R1 is selected from H and carbonyl; R2 is selected from H and X is selected from O and NH; m is an integer of 3-6, and n is 0 or an integer of 3-6. The salidroside derivative has excellent antitumor activity, and the activity is obviously improved compared with salidroside. The preparation method of the salidroside derivative has mild reaction conditions, uses low-toxicity reagents, and has easily-obtained raw materials and convenient post-treatment.

The invention discloses a method for semi-quantitatively and rapidly detecting the content of salidroside based on thin-layer chromatography. The method comprises the following steps: grinding a salidroside medicinal material, putting into a container, adding an organic solvent, carrying out ultrasonic treatment, cooling to room temperature, and filtering to obtain a filtrate which is a sample solution to be detected; the method comprises the following steps: preparing a salidroside standard substance into salidroside standard solutions with different concentration gradients; preparing a developing solvent; taking the salidroside standard solutions with different concentration gradients and the to-be-detected sample solution, and respectively spotting the salidroside standard solutions and the to-be-detected sample solution on a thin-layer plate; placing the thin-layer plate in a developing solvent to obtain a developed thin-layer plate; placing the unfolded thin-layer plate in saturated iodine vapor, taking out the thin-layer plate until spots are clear, and photographing and recording the thin-layer plate to obtain a thin-layer plate image; the correction value of the spot IntDen value is analyzed through thin-layer plate image software, a standard curve is established, and the content of salidroside in the to-be-detected sample solution is obtained in a semi-quantitative mode. The method provided by the invention is beneficial to low-cost, rapid, simple and convenient semi-quantitative detection of low-content salidroside, and is suitable for outdoor field detection.

The present application belongs to the field of solving the problems of poor adhesion in a humid environment, dependence on external stimulation crosslinking and single function of existing oral ulcer treatment materials, and provides a self-oxidation crosslinking sprayable hydrogel as well as a preparation method and application thereof, wherein the hydrogel is PGA-DA@Sal-CDs hydrogel, referred to as PDS Gel, which is composed of polygalacturonic acid (PGA), dopamine (DA) and salidroside-derived carbon dots (Sal-CDs). The hydrogel can realize in-situ gelation without external stimulation, has sprayability and adhesion in a humid environment, and realizes multifunctional integration and synergistic regulation through step-by-step crosslinking strategy and stable network structure, thereby significantly promoting the wound repair process. The hydrogel is applied to the remodeling of the microenvironment of recurrent oral ulcer and acceleration of healing, and the hydrogel is sprayable, self-crosslinking and solidifying, and has anti-inflammatory, antioxidant and antibacterial multifunctional synergistic effects, so as to realize comprehensive regulation of the ulcer microenvironment and promote wound repair.

The invention provides a dental pulp stem cell exosome as well as a preparation method and application thereof, and belongs to the technical field of exosomes. The method comprises the following steps: inoculating exfoliated deciduous tooth pulp stem cells into a serum-free alpha-MEM culture medium containing microparticles, insulin-like growth factors, transforming growth factor-beta1, penicillin and streptomycin for culturing, separating exosomes, dispersing the exosomes into a PBS solution, adding microparticles, propylene glycol and salidroside for incubation, and separating to obtain the high-activity pulp stem cell exosomes. And performing surface modification on alkannic acid and interferon to prepare the dental pulp stem cell exosome. The dental pulp stem cell exosome prepared by the invention has relatively good affinity to a titanium implant material mediated osseointegration repair material, has relatively good advantages of resisting inflammation, regulating immunity, promoting osteogenesis, reducing injury and the like, is simple in preparation method, relatively high in yield and mild in condition, and has a wide application prospect.

The invention discloses a glycosyl transferase mutant and application thereof in preparation of salidroside, and belongs to the technical field of bioengineering. The mutant is obtained by mutating UDP (User Datagram Protocol) glycosyl transferase AtUGT85A1 from arabidopsis thaliana, and the mutant comprises at least one mutation of C128G, F204A and F217V. The invention also discloses a gene for coding the mutant, a recombinant expression vector containing the gene and a genetically engineered bacterium. According to the present invention, the glycosyl transferase mutant with significantly improved catalytic activity is obtained through the structure-oriented rational design, the glycosyl transferase mutant is integrated into the de novo synthesis path of Escherichia coli, and after the constructed gene engineering bacterium is fermented for 72 h, the salidroside titer can achieve 9.1 g / L, and is improved by 200% compared with the wild type; the method provided by the invention gets rid of dependence on exogenous addition of expensive glycosyl donors, has the advantages of simple process, low cost, environmental friendliness and the like, and provides a new strategy for green industrial production of salidroside.

The invention discloses a bacterial-derived glycosyl transferase mutant which is obtained by mutating an amino acid residue at a specified position of an amino acid sequence as shown in SEQ ID No. 1, and discloses a method for catalytically synthesizing salidroside based on the mutant. According to the invention, sucrose synthase GmSUS from soybean and a mutant of glycosyl transferase BparUGT are selected for co-expression to form a UDPG cyclic regeneration system, so that the system can realize cyclic regeneration of UDPG only by using a trace amount of UDP and cheap sucrose, the production cost is greatly reduced, and the scheme better meets industrial application requirements. The optimal mutant and a UDPG circulating system are applied to enzymatic synthesis of the salidroside, the space time yield reaches 2.43 g / L / h and is the highest level reported at present, and the method has industrial application value.

The invention provides a construction method and application of recombinant yarrowia lipolytica with high yield of salidroside, and belongs to the field of bioengineering. The recombinant yarrowia lipolytica with high yield of salidroside is named as a yarrowia lipolytica XJ-SAL strain, and after the recombinant yarrowia lipolytica XJ-SAL strain is subjected to fed-batch fermentation, the salidroside can be synthesized at a relatively high level. The construction method of the recombinant yarrowia lipolytica is easy to operate, and efficient synthesis of salidroside can be achieved.

The invention belongs to the technical field of genetic engineering and microorganisms, and particularly relates to a genetically engineered bacterium for synthesizing hydroxyl salidroside as well as a preparation method and application of the genetically engineered bacterium. The genetically engineered bacterium is obtained by knocking out a PPC enzyme, a TyrR enzyme, a TyrE enzyme, a PheA enzyme and a feaB enzyme on an escherichia coli genome, overexpressing an ACS enzyme, an aceA enzyme, a pck enzyme, a ppsA enzyme, an fbp enzyme, a pgm enzyme, an HpaC enzyme, a galU enzyme and a mutated TyrAfbr enzyme and an AroGfbr enzyme, and introducing an ADH6 enzyme, an ARO10 enzyme, an RrUGT33 enzyme and an HpaB enzyme. The genetically engineered bacterium can efficiently synthesize the hydroxyl salidroside by taking acetate as a unique carbon source, the yield of a 5L fermentation tank reaches 6.3 g / L, the conversion rate is 0.45 g / g, the production cost is remarkably reduced, the substrate toxicity is avoided, and a new strategy is provided for industrial production of the hydroxyl salidroside and high-value utilization of acetate.

The invention discloses a method for improving killing activity of NK cells, and belongs to the technical field of cellular immunity. Carrying out segmented in-vitro culture on the separated NK cells by adopting a specific basic induction culture medium, an activation induction culture medium and an amplification culture medium, wherein recombinant human insulin and sodium selenite are added into the basic induction culture medium; ganoderma lucidum polysaccharides, eicosapentaenoic acid, IL-15 and IL-18 are added into the activation and induction culture medium. Salidroside, tea polyphenol, low molecular weight chitosan, IL-2 (interleukin-2), IL-21 (interleukin-21) and SCF (mesenchymal stem cells) are added into the amplification culture medium. According to the method, the killing ability of the NK cells obtained through in-vitro culture on tumor cells and the in-vitro proliferation effect of the NK cells are remarkably improved, the problems that in the prior art, the in-vitro proliferation efficiency of the NK cells is low, and cell activity is difficult to consider are solved, and a foundation is laid for clinical transformation of NK cell immunotherapy.

The present application provides a salidroside multilayer microcapsule powder comprising salidroside; an inner layer covering the salidroside, the inner layer comprising chitosan or gelatin, and gum arabic; and an outer layer covering the inner layer, the outer layer comprising resistant dextrin, trehalose, and hydrolyzed whey protein or hydrolyzed soy protein. The present application also discloses a food, beverage, nutritional product, dietary supplement, or cosmetic comprising the salidroside multilayer microcapsule powder, and a method for preparing the salidroside multilayer microcapsule powder.