35 results about "Salidroside" patented technology

The invention discloses recombinant escherichia coli capable of producing salidroside and application, exogenous genes are introduced by utilizing a CRISPR / Cas9 technology, the recombinant escherichia coli capable of improving the fermentation yield of the salidroside is obtained by modifying and screening key genes, and finally, the salidroside is obtained by optimizing a fermentation tank process. The recombinant Escherichia coli can reach the highest yield of salidroside produced by fermentation of the scale at present on a 5L fermentation tank. The salidroside is fermented in a 5L fermentation tank for 104 hours, so that the maximum accumulation amount of the salidroside is 33.68 g / L, and industrial enlarged production is facilitated.

The present application provides a rhodiolide composition with a core-shell-shell structure, a preparation method thereof comprising the steps of: 1) EGCG inclusion: adding beta-cyclodextrin and EGCG into water, ultrasonic, forming an inclusion compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, dissolving in chloroform to form a uniform solution; weighing rhodiolide, dissolving in the uniform solution, rotary evaporation to remove chloroform, then adding the inclusion compound and PBS buffer into the container, hydrating, forming a liposome wrapping rhodiolide and inclusion compound; 3) shell loading: weighing collagen, mixing with chitosan solution uniformly to form a complex; adding the complex into the liposome, the complex is coated on the outer layer of the liposome, centrifugal purification, obtaining a rhodiolide composition with a core-shell-shell structure. The present application belongs to the technical field of cosmetic raw materials, and the rhodiolide composition provided has the advantages of high stability, good transdermal effect, and significant anti-aging effect.

The invention provides glycosyl transferase and application thereof in preparation of salidroside, and relates to the technical field of enzyme engineering. The glycosyl transferase FpUGT provided by the invention is derived from fraxinus pennsylvanica, the existence of the glycosyl transferase gene capable of directly catalytically synthesizing the salidroside in the plant is not reported in literatures, and a new biological way is provided for finding the glycosyl transferase for efficiently synthesizing the salidroside. And a new biological material is provided for preparing the salidroside.

The application provides a mutant of acidophilic glycosyltransferase at the 400th position and application thereof in production of salidroside, and belongs to the technical field of bioengineering. The application solves the problem of low synthesis efficiency of salidroside under acidic conditions. The application comprises an acidophilic glycosyltransferase mutant, wherein the glycosyltransferase mutant is obtained by mutating the 400th position of the amino acid shown in SEQ ID NO. 2. The mutant is mainly used for high-yield production of salidroside under acidic conditions.

The invention relates to the technical field of biological medicines, in particular to an exosome for atomization intervention of a plateau pulmonary heart disease and a preparation method and application thereof, the preparation method comprises the following steps: S1, preparing a lung-targeted exosome, and purifying to obtain an azide exosome; s2, carrying out amination modification and activation on the salidroside, so as to obtain DBCO modified salidroside; s3, chemical connection is clicked, and a composite exosome is generated; and S4, purifying the composite exosome, adding a freeze-drying protective agent, uniformly mixing, freeze-drying, and atomizing the freeze-dried exosome for intervening the plateau pulmonary heart disease. Through collaborative design of molecular engineering modification and a preparation process, the exosome system for intervening the plateau pulmonary heart disease by atomization, which has lung targeting capability, a biological activity maintaining function in a plateau low-oxygen environment, structural stability in an atomization process and a freeze-drying-redissolving quick response characteristic, is constructed.

The present application belongs to the field of solving the problems of poor adhesion in a humid environment, dependence on external stimulation crosslinking and single function of existing oral ulcer treatment materials, and provides a self-oxidation crosslinking sprayable hydrogel as well as a preparation method and application thereof, wherein the hydrogel is PGA-DA@Sal-CDs hydrogel, referred to as PDS Gel, which is composed of polygalacturonic acid (PGA), dopamine (DA) and salidroside-derived carbon dots (Sal-CDs). The hydrogel can realize in-situ gelation without external stimulation, has sprayability and adhesion in a humid environment, and realizes multifunctional integration and synergistic regulation through step-by-step crosslinking strategy and stable network structure, thereby significantly promoting the wound repair process. The hydrogel is applied to the remodeling of the microenvironment of recurrent oral ulcer and acceleration of healing, and the hydrogel is sprayable, self-crosslinking and solidifying, and has anti-inflammatory, antioxidant and antibacterial multifunctional synergistic effects, so as to realize comprehensive regulation of the ulcer microenvironment and promote wound repair.

The invention provides a dental pulp stem cell exosome as well as a preparation method and application thereof, and belongs to the technical field of exosomes. The method comprises the following steps: inoculating exfoliated deciduous tooth pulp stem cells into a serum-free alpha-MEM culture medium containing microparticles, insulin-like growth factors, transforming growth factor-beta1, penicillin and streptomycin for culturing, separating exosomes, dispersing the exosomes into a PBS solution, adding microparticles, propylene glycol and salidroside for incubation, and separating to obtain the high-activity pulp stem cell exosomes. And performing surface modification on alkannic acid and interferon to prepare the dental pulp stem cell exosome. The dental pulp stem cell exosome prepared by the invention has relatively good affinity to a titanium implant material mediated osseointegration repair material, has relatively good advantages of resisting inflammation, regulating immunity, promoting osteogenesis, reducing injury and the like, is simple in preparation method, relatively high in yield and mild in condition, and has a wide application prospect.

The invention discloses a glycosyl transferase mutant and application thereof in preparation of salidroside, and belongs to the technical field of bioengineering. The mutant is obtained by mutating UDP (User Datagram Protocol) glycosyl transferase AtUGT85A1 from arabidopsis thaliana, and the mutant comprises at least one mutation of C128G, F204A and F217V. The invention also discloses a gene for coding the mutant, a recombinant expression vector containing the gene and a genetically engineered bacterium. According to the present invention, the glycosyl transferase mutant with significantly improved catalytic activity is obtained through the structure-oriented rational design, the glycosyl transferase mutant is integrated into the de novo synthesis path of Escherichia coli, and after the constructed gene engineering bacterium is fermented for 72 h, the salidroside titer can achieve 9.1 g / L, and is improved by 200% compared with the wild type; the method provided by the invention gets rid of dependence on exogenous addition of expensive glycosyl donors, has the advantages of simple process, low cost, environmental friendliness and the like, and provides a new strategy for green industrial production of salidroside.

The invention discloses a bacterial-derived glycosyl transferase mutant which is obtained by mutating an amino acid residue at a specified position of an amino acid sequence as shown in SEQ ID No. 1, and discloses a method for catalytically synthesizing salidroside based on the mutant. According to the invention, sucrose synthase GmSUS from soybean and a mutant of glycosyl transferase BparUGT are selected for co-expression to form a UDPG cyclic regeneration system, so that the system can realize cyclic regeneration of UDPG only by using a trace amount of UDP and cheap sucrose, the production cost is greatly reduced, and the scheme better meets industrial application requirements. The optimal mutant and a UDPG circulating system are applied to enzymatic synthesis of the salidroside, the space time yield reaches 2.43 g / L / h and is the highest level reported at present, and the method has industrial application value.

The invention provides a construction method and application of recombinant yarrowia lipolytica with high yield of salidroside, and belongs to the field of bioengineering. The recombinant yarrowia lipolytica with high yield of salidroside is named as a yarrowia lipolytica XJ-SAL strain, and after the recombinant yarrowia lipolytica XJ-SAL strain is subjected to fed-batch fermentation, the salidroside can be synthesized at a relatively high level. The construction method of the recombinant yarrowia lipolytica is easy to operate, and efficient synthesis of salidroside can be achieved.

The present application provides a salidroside multilayer microcapsule powder comprising salidroside; an inner layer covering the salidroside, the inner layer comprising chitosan or gelatin, and gum arabic; and an outer layer covering the inner layer, the outer layer comprising resistant dextrin, trehalose, and hydrolyzed whey protein or hydrolyzed soy protein. The present application also discloses a food, beverage, nutritional product, dietary supplement, or cosmetic comprising the salidroside multilayer microcapsule powder, and a method for preparing the salidroside multilayer microcapsule powder.

The application provides use of rhodioloside in preparation of a medicine for preventing and / or treating paclitaxel-induced peripheral neuropathy, and belongs to the technical field of biological medicine. The application provides systematic pharmacodynamic evidence and molecular mechanism interpretation for prevention and treatment of paclitaxel-related peripheral neuropathy, and is expected to be developed into a new candidate medicine for preventing and / or treating paclitaxel-induced peripheral neuropathy, and has good clinical transformation prospect.

The invention discloses a whole-process quality transmission quality control method of a mixture capable of tonifying qi, soothing nerves and eliminating diseases. The method comprises the following steps: respectively preparing a test sample in four process stages of decoction, concentration, centrifugation and sterilization, measuring seven physical parameters including pH, refractive index, dynamic viscosity, conductivity, total amount of soluble solids, solid content and density, and constructing a physical fingerprint spectrum; the method comprises the following steps: determining the contents of nine index components including salidroside, caffeic acid, ferulic acid, specnuezhenide, naringin, hesperidin, rosmarinic acid, formononin and nobiletin by adopting an HPLC (High Performance Liquid Chromatography) method, establishing a chemical fingerprint spectrum, and calculating the transfer rate of each component in each process stage; the correlation between the physical parameters and the component transfer rate is analyzed through the matrix hotspot map, and common peaks are identified to calculate the similarity. A multi-index evaluation mode of physical fingerprint, chemical fingerprint and multi-component content determination is established, the influence of the preparation process on the overall quality of the traditional Chinese medicine can be systematically evaluated, and a scientific basis is provided for quality control in the traditional Chinese medicine production process.

The invention discloses recombinant corynebacterium glutamicum for producing salidroside and application of the recombinant corynebacterium glutamicum, and belongs to the field of genetic engineering and bioengineering. Through expression regulation and fermentation condition optimization, the method for producing salidroside at high yield by using corynebacterium glutamicum is constructed. According to the method, in the salidroside production process, carbon flow cannot be accumulated in tyrosol, and the yield of salidroside in shake flask fermentation of a minimum culture medium can reach 2.1 g / L.

The invention discloses a nourishing and repairing emollient and a preparation method thereof. In a basic nourishing system, prinsepia utilis royle oil and Zhejiang red camellia seed oil have a synergistic effect, rich unsaturated fatty acids and fat-soluble nutrients are provided, the nourishing power is enhanced, and the skin feeling is improved; and sufficient natural beeswax and soybean-derived phytosterol improve the stability of the system and strengthen the synergistic interaction. The active repairing system adopts high-purity fermentation type salidroside and glabridin with the purity of 99%, so that the bioavailability and repairing efficiency of active components are improved; the low-molecular sodium hyaluronate is matched with the glycerol, so that the moisturizing infiltration capacity is enhanced, and the deep moisturizing of the skin is promoted. In the auxiliary synergistic system, rose hydrolat endows a natural soothing effect, soya bean lecithin guarantees emulsion stability, tocopherol and allantoin are compounded to strengthen antioxidant and repair soothing effects, and product stability, activity retention rate, skin feeling in use and safety are comprehensively improved.

The invention relates to the technical field of biology, in particular to a method for preparing extracellular vesicles based on a bionic microenvironment, the extracellular vesicles and application. The method provided by the invention can be adapted to a bioreactor to realize large-scale culture of umbilical cord mesenchymal cells and large-scale production of extracellular vesicles; meanwhile, the in-vivo low-oxygen physiological state and the 3D microenvironment are simulated by adding a bionic microenvironment in which the salidroside and the cell-ECM interact, and the repairing and anti-inflammatory effects of the extracellular vesicles are effectively improved. In a bionic microenvironment constructed by adopting the method provided by the invention, cells are colonized in a microcarrier and secrete ECM to form a'cell-ECM 'composite structure, and in combination with simulation of a hypoxia physiological state, HIF-1alpha gene expression is effectively up-regulated, directional metabolism of the cells is promoted, extracellular vesicles are rich in repair and anti-inflammatory related active ingredients IL-10, the repair and anti-inflammatory effects are outstanding, and the repair and anti-inflammatory effects are remarkable. The method is suitable for wide popularization and application.

The invention belongs to the technical field of anti-aging of mesenchymal stem cells, and particularly relates to a preparation and method for preventing replicative aging of mesenchymal stem cells, the preparation comprises salidroside, semen cuscutae total flavonoids and ergothioneine, and the method comprises the following steps: in the in-vitro amplification process of the mesenchymal stem cells, culturing under the hypoxia condition of 2-5% O2, the preparation for preventing the replicative aging of the mesenchymal stem cells has the advantages that the combination of effective components of Chinese herbal medicines is combined with a low-oxygen condition, the replicative aging of the mesenchymal stem cells is prevented, and the preparation is safer, more efficient, low in cost and capable of realizing large-scale amplification.

The utility model discloses a salidroside impurity removal equipment, including the disc centrifuge for carrying out the filtration to salidroside feed liquid, the upper portion of disc centrifuge is provided with C shape frame, is set up with through -hole on C shape frame, the inside rotation of C shape frame is connected with filter carousel, and filter carousel sets up with several groups filter installation hole, and several groups filter installation hole is annular array setting and one group filter installation hole is coincident with through -hole, and the top of C shape frame has the feed pipe, and the inner chamber of feed pipe is linked together with through -hole, and the feed end of feed pipe is linked together with the pipeline for conveying salidroside feed liquid, through the setting of disc centrifuge and preliminary filter piece, disc centrifuge improves the processing capacity, reaches the continuous production state, and the energy consumption is lower than membrane filter, simultaneously, the maintenance of disc centrifuge mainly concentrates in the cleaning and dynamic balance check of rotary drum etc.

Use of glucosyltransferase U8GT3 and / or poppy-derived tyrosine decarboxylase or a mutant thereof in yeast synthesis of salidroside is provided, as is use of poppy-derived tyrosine decarboxylase or a mutant thereof in yeast synthesis of tyrosol. The amino acid sequences of the poppy-derived tyrosine decarboxylase and its mutant are set forth in SEQ ID NOs: 7 to 8, respectively, and the nucleotide sequences of their encoding genes are set forth in SEQ ID NOs: 1 to 2, respectively. The amino acid sequence of glucosyltransferase U8GT3 is set forth in SEQ ID NO: 10, and the nucleotide sequence of its encoding gene is set forth in SEQ ID NO: 4.

The application provides a mutant of an acidophilic glycosyltransferase and application thereof in production of salidroside, and belongs to the technical field of bioengineering. The application solves the problem of low synthesis efficiency of salidroside under acidic conditions. The application comprises a mutant of an acidophilic glycosyltransferase, wherein the glycosyltransferase mutant is obtained by mutating any one of positions 87, 210, 293, 356, 399, 400 and 415 of the amino acid sequence shown in SEQ ID NO. 2. The mutant is mainly used for high-yield production of salidroside under acidic conditions.

The invention discloses a method for simultaneously determining nine index components in a Zhibeng bacteriostatic liquid and constructing a characteristic chromatogram and application, and belongs to the technical field of detection and analysis. Octadecylsilane chemically bonded silica is used as a filling agent; 0.1% phosphoric acid water is used as a mobile phase A, and acetonitrile is used as a mobile phase B; the detection wavelength 316 nm is used for establishing a characteristic spectrum and determining the content of cimicifugin and liquiritin, the detection wavelength 402 nm is used for determining the content of hydroxysafflor pigment A and dehydrated safflower yellow B, the detection wavelength 254 nm is used for determining the content of ganoderic acid A and schizandrin, and the detection wavelength 275 nm is used for determining the content of lozastilbin, salidroside and 5-hydroxymethylfurfural. According to the method, separation and accurate detection are realized by adopting a gradient elution procedure. So far, no report about simultaneous determination of the nine components is found, and the method makes up the blank of quality control and fingerprint spectrum research in prescriptions containing the medicinal materials.

The invention provides a glycosyl transferase AtUGT85A1 mutant and application of the glycosyl transferase AtUGT85A1 mutant in biosynthesis of salidroside, and belongs to the technical field of bioengineering. According to the invention, the problem of low salidroside yield caused by poor solubility of glycosyl transferase AtUGT85A1 in the prior art is solved. The salidroside comprises a glycosyl transferase mutant, the glycosyl transferase mutant takes an amino acid sequence as shown in SEQ ID NO.1 as a starting sequence, the solubility of the glycosyl transferase mutant in a fermentation environment is improved on the premise of keeping the catalytic capability, and a foundation is laid for producing salidroside by a microbial fermentation method.

The invention discloses a glycosyl transferase mutant and application thereof in production of salidroside, and relates to the technical field of biology. The glycosyl transferase mutant is any one of A1) to A2): A1) a protein obtained by performing amino acid mutation on at least one of the 41st site, the 114 site, the 272th site and the 340th site of an amino acid sequence of wild-type glycosyl transferase; a2) is a fusion protein obtained by connecting a label to the N end or / and C end of the protein shown in A1), and the amino acid sequence of the wild type glycosyltransferase is shown in SEQ ID NO: 1. The wild type glycosyltransferase can efficiently catalyze tyrosol to generate salidroside.

The invention provides fermented tobacco leaves and a preparation method thereof. The preparation method of the fermented tobacco leaves comprises the following steps: mixing a glossy privet fruit extract with cigar tobacco leaves to obtain a mixture; and performing fermentation treatment on the mixture to prepare the fermented tobacco leaves. Through research, the inventor finds that the ligustrum lucidum ait extract and the cigar tobacco leaves are mixed and fermented, the ligustrum lucidum ait extract and the cigar tobacco leaves are rich in ligustroside, salidroside, ligustrum lucidum ait flavonoid glycoside and oleuropein, and the maturity and sensory quality of the cigar tobacco leaves can be obviously improved.

A composition and a method for use in neuroprotection or improving cognitive ability, enhancing athletic performance or improving muscle function, combatting inflammation, combatting aging, extending lifespan or improving healthspan, managing stress or improving mood. The composition comprises: salidroside or a pharmaceutically acceptable salt, ester, or derivative thereof; and ergothioneine or a pharmaceutically acceptable salt, acid, ester, analog, or derivative thereof.

The invention discloses a cytochrome oxidase P450 BM3 mutant and application thereof, and belongs to the technical field of bioengineering. According to the invention, the cytochrome oxidase P450 BM3 derived from bacillus megatherium is subjected to site-directed mutagenesis to obtain the cytochrome oxidase P450 BM3 mutant with obviously improved catalytic activity and selectivity, and when the 2-phenethyl alcohol derivative is prepared, the P450 BM3 mutant can obviously improve the yield of the 2-phenethyl alcohol derivative; furthermore, cells for efficiently synthesizing the 2-phenethyl alcohol derivative from the beginning by taking glucose as a substrate are constructed, the high yield of the 2-phenethyl alcohol derivative is realized, the highest yield of the (R)-phenethyl alcohol derivative is 3.54 g / L, and the final yield is increased to 12.4 g / L through fed-batch fermentation of a 5L fermentation tank, which is the highest yield of the (R)-phenethyl alcohol produced by known microorganisms at present; and the yield of the salidroside reaches 546.20 mg / L.

The present application relates to the field of bioengineering technology, and particularly relates to a recombinant bacillus subtilis, a construction method thereof and application of the bacillus subtilis in producing tyrosol and salidroside, comprising the following steps: S1, extracting parsley and / or rose leaves, and a whole genome of the bacillus subtilis; S2, designing primers for the tyrosine decarboxylase of the parsley, the benzaldehyde synthase of the rose and the bacillus subtilis YjiA respectively as target genes, and performing PCR amplification on the target gene fragments; S3, connecting the target gene fragments with an expression vector to construct a recombinant vector; S4, transforming the recombinant vector into the bacillus subtilis, and screening the transformants by using a resistance gene to obtain a recombinant bacillus subtilis strain. The present application successfully constructs the bacillus subtilis engineering bacteria for heterologous synthesis of tyrosol and salidroside, provides a new idea for industrial fermentation production of tyrosol and salidroside, and lays a foundation for environment-friendly production of tyrosol and salidroside.

The application belongs to the technical field of hydrogel microneedles, and relates to a hydrogel microneedle and a preparation method thereof, and the preparation method comprises the following steps: placing a methacrylated chitosan solution in a centrifugal tube, adding a photoinitiator, vortexing and ultrasonicating until the photoinitiator is completely dissolved to obtain a hydrogel microneedle filling solution; and taking the hydrogel microneedle filling solution and injecting the hydrogel microneedle filling solution into a PDMS microneedle mold, centrifuging, irradiating with an ultraviolet lamp and drying.The hydrogel microneedle prepared from methacrylated chitosan has superior performance in wound closure, re-epithelialization and hair follicle formation, and the hydrogel microneedle is combined with a rhodioside drug depot to construct a transdermal drug delivery system, the drug delivery system has high drug loading and no polymer deposition, can efficiently transdermally deliver rhodioside, promotes wound closure and tissue remodeling, and provides a potential strategy for wound treatment.

The application provides a composition which comprises at least ergothioneine, rhodioloside and coenzyme Q10 in a physiologically acceptable medium, and the three substances in the composition are synergistically compounded to achieve the effect of significantly repairing myocardial injury and protecting cardiovascular.

The invention discloses application of salidroside in preparation of a medicine for enhancing sensitivity of lung cancer combined diabetes chemotherapy. The application firstly researches and verifies that the salidroside can enhance the sensitivity of lung cancer combined with diabetes to chemotherapeutic drugs, and particularly, the salidroside can obviously enhance the sensitivity of lung cancer combined with diabetes to paclitaxel, so that the anti-tumor treatment effect is improved; a new treatment strategy is provided for optimization of a chemotherapy regimen of a patient with lung cancer combined with diabetes in clinic, a new candidate active component is also provided for preparation of an adjuvant therapy drug for enhancing chemotherapy sensitivity, and the application has important value and significance in improvement of treatment prognosis of the patient with lung cancer combined with diabetes.