127 results about "Salidroside" patented technology

The invention provides application of acidophilous glycosyltransferase in salidroside production, and belongs to the technical field of biological engineering. The problem of producing salidroside under the acidic condition is solved. Comprising an application of acidophilous glycosyl transferase with an amino acid sequence as shown in SEQ ID NO.1 in salidroside production under an acidic condition and an acidophilous escherichia coli engineering strain for producing salidroside. The escherichia coli engineering strain overexpresses a mutant 3-deoxy-D-arabinoheptulose-7-phosphate (DAHP) synthetase gene aroGfbr, a cyclohexadiene dehydrogenase gene tyrC, a glucose phosphate mutant enzyme gene pgm and a UDP-glucose pyrophosphorylase galU, overexpresses a phenylpyruvate decarboxylase gene ARO10 derived from saccharomyces cerevisiae, and can be used for producing a mutant 3-deoxy-D-arabinoheptulose-7-phosphate mutant enzyme. The kit comprises an ethanol dehydrogenase gene ADH6 and a glycosyl transferase gene LrUGT85AF8. The method is mainly used for producing salidroside under an acidic condition.

According to the present invention, glycosyl transferase UGTBL1 derived from Bacillus licheniformis ZSP01 is adopted as a research object, AlphaFold 2 is adopted to carry out modeling, molecular docking, molecular dynamics simulation and other technologies, amino acids in an active pocket region of glycosyl transferase are analyzed, site-directed mutagenesis is performed on the amino acids to obtain a series of glycosyl transferase mutants, and salidroside can be highly selectively catalyzed and synthesized.

The invention discloses a method for detecting the content of salidroside in a rhodiola rosea extracting solution based on artificial intelligence. The method comprises the following steps: S1, performing spectral scanning of different wavebands on an extracting solution sample in a plurality of near-infrared wavelength ranges; s2, performing multi-dimensional interference suppression processing and signal purification; s3, carrying out spectral feature compression calculation to obtain a salidroside feature intensity factor; s4, carrying out salidroside characteristic signal-to-noise ratio enhancement and purity index calculation; s5, predicting the content of salidroside and outputting a content prediction score; and S6, mapping the content prediction score into the final salidroside content. According to the method, spectral signal purification, nonlinear feature extraction, dynamic noise enhancement, artificial intelligence prediction modeling and a secondary recheck mechanism are utilized to realize adaptive analysis of extracting solutions from different sources, so that the detection accuracy and stability are remarkably improved, and rapid and traceable salidroside content detection can be realized under the condition that a large precise instrument is not needed.

The present invention provides an anti-aging, whitening, freckle-removing, moisturizing skin care water and a preparation process thereof. A preparation process for an anti-aging, whitening, freckle-removing, moisturizing skin care water comprises the following steps: supercritical CO2 extraction of oat-yarrow extract; ultrasonic-enzymatic hydrolysis-assisted reflux extraction and concentration; adding oxidized resveratrol, salidroside and ellagic acid; adding purified water to dissolve and adding sodium alginate and glycerol; adding p-anisic acid and aromatic alcohol and homogenizing and mixing. The present invention is a skin care water prepared by first extracting extracts of bletilla striata, wormwood leaf, peony bark, oat and yarrow, and then adding oxidized resveratrol, salidroside, ellagic acid, sodium alginate, glycerol, p-anisic acid and aromatic alcohol. The skin care water not only has the effects of anti-aging, whitening, freckle removal and moisturizing, but also because its raw materials are all natural plant ingredients and no chemical agents are added, therefore, it will not irritate the skin and has no side effects, and can be used for a long time.

The invention particularly relates to EGCG (epigallocatechin gallate) induced collagen modified salidroside as well as a preparation method and application thereof. The invention provides a method for modifying salidroside by inducing collagen through EGCG (epigallocatechin gallate). The method comprises the following steps: S1, preparing an EGCG solution; s2, preparing a collagen solution; s3, preparation of EGCG (Epigallocatechin Gallate)-collagen microspheres; s4, preparing a salidroside solution; and S5, modifying the salidroside solution. According to the present invention, the EGCG is adopted to induce the collagen to self-assemble so as to form the microspheres, and the microspheres are used to modify the salidroside, such that the stability, the solubility and the biological activity of the salidroside are significantly improved, and the application range of the salidroside product is further expanded.

The invention discloses recombinant escherichia coli capable of producing salidroside and application, exogenous genes are introduced by utilizing a CRISPR / Cas9 technology, the recombinant escherichia coli capable of improving the fermentation yield of the salidroside is obtained by modifying and screening key genes, and finally, the salidroside is obtained by optimizing a fermentation tank process. The recombinant Escherichia coli can reach the highest yield of salidroside produced by fermentation of the scale at present on a 5L fermentation tank. The salidroside is fermented in a 5L fermentation tank for 104 hours, so that the maximum accumulation amount of the salidroside is 33.68 g / L, and industrial enlarged production is facilitated.

The invention provides application of enhanced mesenchymal stem cells and exosomes in chronic nephritis and renal failure, and belongs to the technical field of biological medicine manufacturing. Through composite pretreatment of salidroside and astragalus polysaccharide, the treatment potential of exosomes secreted by the mesenchymal stem cells is remarkably enhanced, a new thought is provided for intervention of the chronic kidney diseases, and various pathologies of the chronic kidney diseases are covered. In addition, a 3D microenvironment constructed by fibrin gel is combined to simulate an in-vivo extracellular matrix, the natural growth state of the stem cells is better fitted, directional differentiation and function regulation of the stem cells are promoted, and therefore the biological activity and targeting performance of the exosome are improved.

The present application provides a rhodiolide composition with a core-shell-shell structure, a preparation method thereof comprising the steps of: 1) EGCG inclusion: adding beta-cyclodextrin and EGCG into water, ultrasonic, forming an inclusion compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, dissolving in chloroform to form a uniform solution; weighing rhodiolide, dissolving in the uniform solution, rotary evaporation to remove chloroform, then adding the inclusion compound and PBS buffer into the container, hydrating, forming a liposome wrapping rhodiolide and inclusion compound; 3) shell loading: weighing collagen, mixing with chitosan solution uniformly to form a complex; adding the complex into the liposome, the complex is coated on the outer layer of the liposome, centrifugal purification, obtaining a rhodiolide composition with a core-shell-shell structure. The present application belongs to the technical field of cosmetic raw materials, and the rhodiolide composition provided has the advantages of high stability, good transdermal effect, and significant anti-aging effect.

The invention discloses application of salidroside in preparation of a preparation for resisting virus infection of macrobrachium rosenbergii, and relates to the field of antiviral application. The salidroside is injected into the macrobrachium rosenbergii infected with the DIV1 virus, so that the accumulative survival rate of the macrobrachium rosenbergii infected with the DIV1 virus can be remarkably increased, the replication capacity of the DIV1 virus is inhibited, and the relative expression quantity of antiviral genes is increased; the salidroside can be used as a medicine or a feed additive for preventing or treating antiviral infection of macrobrachium rosenbergii.

The invention provides a salidroside composition with a core-shell-shell structure, and a preparation method of the salidroside composition comprises the following steps: 1) EGCG (epigallocatechin gallate) clathration: adding beta-cyclodextrin and EGCG into water, and carrying out ultrasonic treatment to form a clathrate compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, and dissolving in chloroform to form a uniform solution; the preparation method comprises the following steps: weighing salidroside, dissolving the salidroside in a uniform solution, carrying out rotary evaporation to remove chloroform, then adding an inclusion compound and a PBS (Phosphate Buffer Solution) into a container, and hydrating to form lipidosome wrapping the salidroside and the inclusion compound; 3) shell loading: weighing collagen, and uniformly mixing the collagen with the chitosan solution to form a compound; the salidroside composition with the core-shell-shell structure is obtained by dropwise adding the compound into the lipidosome, coating the outer layer of the lipidosome with the compound and carrying out centrifugal purification. The invention belongs to the technical field of cosmetic raw materials, and provides a salidroside composition which has the advantages of high stability, good transdermal effect, remarkable anti-aging effect and the like.

The invention belongs to the technical field of cell culture, and particularly relates to a serum-free mesenchymal stem cell culture medium and a preparation method thereof. The serum-free mesenchymal stem cell culture medium provided by the invention is prepared by taking a DMEM (Dulbecco Modified Eagle Medium) / F12 basal culture medium as a basis and adding supplementary factors, namely yeast hydrolysate, insulin, albumin, dexamethasone, taurine, salidroside, soy isoflavone, baicalein, human platelet lysate, momordica saponin and hyperoside. According to the serum-free mesenchymal stem cell culture medium provided by the invention, the normal form of cells can be maintained without supplementing serum, rapid proliferation is realized, and the biological characteristics of the mesenchymal stem cells are maintained.

The invention relates to the technical field of biosynthesis of salidroside, in particular to a metabolic engineering modified tobacco BY2 cell and application of the metabolic engineering modified tobacco BY2 cell in production of salidroside. The metabolic engineering modified tobacco BY2 cell is constructed according to the following method: S1, connecting nucleotide sequences of an Rr4HPAAS gene, an Rr4HPAR gene and an RrUGT33 gene in series, and inserting into an expression vector to obtain a recombinant plasmid pK7FGW2-HHU; and S2, transferring the recombinant plasmid pK7FGW2-HHU into a tobacco BY2 cell through an agrobacterium tumefaciens mediated method, so as to obtain the metabolic engineering modified tobacco BY2 cell. The invention provides a metabolic engineering modified tobacco BY2 cell and application of the metabolic engineering modified tobacco BY2 cell in salidroside production, and aims to reconstruct a salidroside metabolic pathway, realize salidroside heterologous production and reduce the production cost of salidroside.

The invention discloses a culture method for hypoxia culture of umbilical cord mesenchymal stem cells, which comprises the following steps: hypoxia pre-adaptive culture: inoculating umbilical cord mesenchymal stem cells in a culture medium containing bFGF (basic fibroblast growth factor), glutathione, astragalus polysaccharide and salidroside, and transferring the umbilical cord mesenchymal stem cells into a 5% O2 environment after the umbilical cord mesenchymal stem cells are preliminarily adhered to the wall in a normal oxygen environment; performing low-oxygen enrichment culture: replacing a second culture medium containing astragalus polysaccharide, salidroside, D-ribose, sodium pyruvate and D-glucose, and promoting efficient cell proliferation under 2% O2; performing function strengthening culture: adding tanshinone IIA and GSK-269962A, inducing cells to secrete VEGF under 1% O2, and maintaining high dryness. According to the method, through the synergistic effect of the three-stage gradient oxygen concentration and the specific serum-free culture medium, the cell proliferation multiple is remarkably increased, the total apoptosis rate is reduced, VEGF secretion is promoted, the multidirectional differentiation potential is reserved, the culture method is standardized in operation, the serum-free culture medium is definite in component, and a high-quality stem cell culture scheme is provided for the field of regenerative medicine.

The invention discloses a composition for preventing and treating plateau erythrocythemia and application of the composition, and relates to the technical field of pharmaceutical composition preparation. The composition is prepared from PT2385 and salidroside. The combination of PT2385 and salidroside can effectively inhibit hyperplasia of red blood cells of HAPC rats, reduce hemoglobin and relieve blood flow stasis and microcirculation disturbance of body tissues and organs caused by hypoxia, so that hypoxia phenotypic characteristics of the HAPC rats are improved. In addition, free radicals can be effectively removed, oxidative stress, peroxidation reaction and inflammatory reaction induced by hypoxia are inhibited, the mitochondrial function is protected, and the dysfunction of HAPC rat tissue and organ injury is improved. The problem that in the prior art, an effective medicine for preventing and treating plateau erythrocythemia is lacked is solved.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium, which is composed of a basic culture medium and additive components, and the additive components comprise, by final concentration, 5-20 [mu] g / mL of astragalus polysaccharide, 0.5-2.0 [mu] g / mL of salidroside, 0.1-0.5 [mu] g / mL of tanshinone IIA, 0.1-0.3 mg / mL of sodium pyruvate, 5-15 mM of D-ribose, 3-6 mg / mL of recombinant human albumin, and 5-10 [mu] g / mL of recombinant transferrin; the invention also discloses a method for culturing umbilical cord mesenchymal stem cells under the hypoxia condition by using the serum-free culture medium. The method comprises the steps of cell inoculation, culture under the hypoxia condition, subculture, cell passage and the like. The astragalus polysaccharide, the salidroside, the tanshinone IIA, the sodium pyruvate, the recombinant human albumin and the recombinant transferrin are added into the serum-free culture medium, so that nutrition supply and function maintenance of cells under a low-oxygen condition are guaranteed, the proliferation rate and the differentiation capacity of the cultured cells are improved, the apoptosis rate of the cells is reduced, and the survival rate of the cells is increased. Wide application prospects are realized in the fields of cell culture, regenerative medicine and the like.

The application discloses a mouse wearable micro-injection device for slow release of rhodiolin, and relates to the technical field of medical miniaturized devices. The device comprises a fixing frame, a slow injection device arranged at the upper end of the fixing frame, and a flow guide device connected with the slow injection device. The slow injection device comprises a syringe body internally provided with a liquid storage area and a reaction area. An injection plug is arranged between the liquid storage area and the reaction area. The side edge of the injection plug is in close contact with the inner wall of the syringe body. The flow guide device is in communication with the liquid storage area. The device can realize accurate control of the drug release rate through three different reaction mechanisms, i.e. gas driving of a drug bin, physical expansion of an expansion bag and composite expansion of an expansion bin. In addition, the design of the hanging ear port in the fixing frame can not only disperse the pressure on the ears, but also reduce the fluctuation range of the mouse's corticosterone level in combination with the lightweight structure design.

The invention provides glycosyl transferase and application thereof in preparation of salidroside, and relates to the technical field of enzyme engineering. The glycosyl transferase FpUGT provided by the invention is derived from fraxinus pennsylvanica, the existence of the glycosyl transferase gene capable of directly catalytically synthesizing the salidroside in the plant is not reported in literatures, and a new biological way is provided for finding the glycosyl transferase for efficiently synthesizing the salidroside. And a new biological material is provided for preparing the salidroside.

The invention discloses a method for extracting and purifying salidroside from salidroside fermentation liquor, which comprises the following steps: (1) adding tert-butyl alcohol into the salidroside fermentation liquor, filtering, and collecting clear liquid; (2) concentrating the clear solution obtained in the step (1) under reduced pressure to obtain a concentrated solution, adding petroleum ether for extraction, and collecting a water phase; (3) adding ammonium sulfate into the water phase obtained in the step (2), uniformly mixing, adjusting the pH to be acidic, adding ethyl acetate for extraction, and collecting an organic phase; and (4) concentrating the organic phase obtained in the step (3) under reduced pressure, and drying. Through a solvent polarity gradient matching technology and a salting-out enhanced phase separation mechanism, the structural stability of the salidroside is guaranteed, meanwhile, the extraction and purification technological process is simplified, the separation efficiency is remarkably improved, the purity and yield of the salidroside obtained through extraction and purification with the method are both larger than or equal to 90%, and the method is suitable for industrial production. The extraction and purification process provided by the invention is also provided with a solvent recovery system, and the efficiency and economy of industrial production are both considered.

The invention discloses low-caffeine nerve stimulation tea wine and a preparation method thereof, the low-caffeine nerve stimulation tea wine is prepared by blending eucommia wood aged tea wine, cherry wood aged tea wine and cherry wood / Hainan dalbergia odorifera wood aged tea wine with different baking degrees, and the mass ratio of geniposidic acid to salidroside to formononetin in the wine body is (2-30): (1-15): 1. The mass ratio of the genkwanin to the alpinia japonica to the sesamol is (0.5-8): (0.15-6): 1, the mass ratio of the isoliquiritigenin to the vitexin to the syringaldehyde is 1: (0.15-5): (4-18), and the total content of the geniposidic acid, the salidroside, the formononetin, the genkwanin, the alpinia japonica, the sesamol, the isoliquiritigenin, the vitexin and the syringaldehyde in the wine is 40-250 mg / 100 mL. By combining and matching the functional components in the wood and the proportion of the functional components, the antagonistic effect on caffeine nerve stimulation in the tea wine is achieved.

The present invention relates to a salidroside derivative, specifically a hydroxy-α-salidroside compound. The hydroxy-α-salidroside compound is superior to α-salidroside in scavenging DPPH free radicals, whitening skin, and improving learning and memory disorders. The compound can be added as a new functional ingredient to cosmetics, functional foods, or pharmaceutical compositions. The preparation method of the hydroxy-α-salidroside of the present invention uses α-salidroside as a substrate and synthesizes hydroxy-α-salidroside under the action of hydroxylase.

The invention discloses multifunctional composite hydrogel for integrally treating infected wounds as well as a preparation method and application of the multifunctional composite hydrogel, and belongs to the technical field of medicines. The multifunctional composite hydrogel for integrally treating infected wounds is prepared from the following raw materials: polyhexamethylene biguanide grafted silk fibroin, acrylamide, N-isopropylacrylamide, salidroside, N, N-dimethylformamide, polyhexamethylene biguanide grafted polyhexamethylene biguanide grafted polyhexamethylene biguanide grafted polyhexamethylene biguanide grafted polyhexamethylene biguanide grafted polyhexamethylene biguanide grafted polyhexamethylene The composite hydrogel is prepared from N, N '-methylene bisacrylamide and water, has multiple functions including high air permeability, high tensile resistance, high adhesion, injectability, self-healing property and multiple responsiveness, and meets the mechanical property requirement of wound dressing and the biological requirement for promoting healing of infected wounds. The composite hydrogel has treatment effects in different stages of wound healing, and can integrally and efficiently solve the problems of slow healing of infected wounds, high scar formation risk and the like.

The invention discloses application of a combination of scutellarin and salidroside in treatment of solar dermatitis, finds that the combination of scutellarin and salidroside has a synergistic effect on treatment of solar dermatitis for the first time, and provides a brand new and effective drug combination strategy for treatment of solar dermatitis. A new thought is provided for research and development of anti-solar dermatitis drugs, and the application value and the industrial conversion prospect are good.

The application provides a mutant of acidophilic glycosyltransferase at the 400th position and application thereof in production of salidroside, and belongs to the technical field of bioengineering. The application solves the problem of low synthesis efficiency of salidroside under acidic conditions. The application comprises an acidophilic glycosyltransferase mutant, wherein the glycosyltransferase mutant is obtained by mutating the 400th position of the amino acid shown in SEQ ID NO. 2. The mutant is mainly used for high-yield production of salidroside under acidic conditions.

The invention discloses a method for producing high-purity salidroside from fermentation liquor through green purification, and belongs to the field of salidroside. The invention aims to solve the problems of high energy consumption, high pollution and low yield of purification processes such as decoloration, extraction, crystallization and freeze-drying by adopting adsorbents such as activated carbon in the existing salidroside separation and purification technology. The method comprises the following steps: carrying out solid-liquid separation on salidroside fermentation liquor through ceramic membrane microfiltration; then carrying out ultrafiltration by using an ultrafiltration membrane; diluting with purified water to serve as a chromatography loading solution, and adsorbing with a macroporous adsorption resin column with a non-polar styrene skeleton for primary decoloration and purification; then carrying out vacuum concentration; dissolving with ethanol, directly loading to an aluminum oxide chromatographic column for secondary decoloration, and eluting with 100%-80%-50% ethanol after loading is finished; concentrating under reduced pressure, and refining through a polymer filler until the purity is greater than or equal to 99.5%; and performing vacuum concentration and spray drying. Technical support and high-quality raw materials can be provided for the industries such as medicine, cosmetics and functional food.

The present application relates to a kind of selectively improved glycosyltransferase UGTBL1 mutant and its catalytic synthesis of application of salidroside, belong to genetic engineering and enzyme engineering field.The mutant obtained by single point mutation technology includes the amino acid sequence of 132th amino mutation, the amino acid sequence of the glycosyltransferase is as shown in SEQ ID NO:1.The research results show that the glycosyltransferase mutant S132V, S132G described in the application has good selectivity, can efficiently catalyze tyrosol and uridine diphosphate glucose to synthesize salidroside, and the selectivity is 89%, suitable for the enzyme preparation of salidroside, with wide application prospect.

The invention relates to the technical field of biological medicines, in particular to an exosome for atomization intervention of a plateau pulmonary heart disease and a preparation method and application thereof, the preparation method comprises the following steps: S1, preparing a lung-targeted exosome, and purifying to obtain an azide exosome; s2, carrying out amination modification and activation on the salidroside, so as to obtain DBCO modified salidroside; s3, chemical connection is clicked, and a composite exosome is generated; and S4, purifying the composite exosome, adding a freeze-drying protective agent, uniformly mixing, freeze-drying, and atomizing the freeze-dried exosome for intervening the plateau pulmonary heart disease. Through collaborative design of molecular engineering modification and a preparation process, the exosome system for intervening the plateau pulmonary heart disease by atomization, which has lung targeting capability, a biological activity maintaining function in a plateau low-oxygen environment, structural stability in an atomization process and a freeze-drying-redissolving quick response characteristic, is constructed.

The invention relates to application of alpha-glucosidase in synthesis of salidroside, and belongs to the technical field of biosynthesis. According to the technical scheme, the application of the alpha-glucosidase in the aspect of synthesizing the alpha-salidroside is characterized in that the alpha-glucosidase is derived from Ensifer adhaerens, the amino acid sequence of the alpha-glucosidase is shown in SEQ ID No: 1, and the Ensifer adhaerens is CCTCC AB 2019220. The invention provides an alpha-salidroside preparation technology which is simple and convenient in preparation process, high in green safety and low in production cost, and industrial large-scale production is facilitated.

The invention discloses a staying-up glowing composition containing multiple plant extracts. The staying-up glowing composition containing the multiple plant extracts is prepared from the following components in parts by mass: 3 to 5 parts of extract A, 10 to 15 parts of extract B, 2 to 4 parts of extract C, 1.5 to 3 parts of curcumin nano-liposome, 2 to 5 parts of lactobacillus fermentation filtrate, 1 to 3 parts of water-locking magnetite, 0.1 to 0.3 part of xanthan gum / acrylate compound thickening agent and 0.5 to 1 part of preservative composition. According to the staying up glowing composition containing the multiple plant extracts, the target component of the extract A is jasmonic acid methyl ester, the target component of the extract B is salidroside and nausea acid, and the curcumin nano-liposome is compounded, so that the staying up glowing composition containing the multiple plant extracts is obtained; compared with similar products sold in the market at present, the anti-oxidation and anti-saccharification capacities of the staying-up glowing composition can be enhanced in a targeted manner, the transdermal efficiency is improved, and meanwhile, the DNA repair effect and the anti-inflammatory effect are improved.

According to the application of the salidroside in preparation of the medicine for treating the Alzheimer's disease by relieving ferroptosis, the salidroside is used for relieving the ferroptosis of human neuroblastoma (SH-SY 5Y) cells induced by A [beta] 1-42 by inhibiting a voltage dependent anion channel 1 (VDAC1) and activating an AKT / GSK-3beta pathway, and the application of the salidroside in the preparation of the medicine for treating the Alzheimer's disease by relieving the ferroptosis of the human neuroblastoma (SH-SY 5Y) is realized by inhibiting the voltage dependent anion channel 1 (VDAC1) and activating the AKT / GSK-3beta pathway. The salidroside can relieve the ferroptosis of SH-SY 5 Y cells induced by A beta 1-42 under the optimal concentration of 30 mM. After salidroside treatment, the expression quantity of GPX 4 and FTH 1 is increased, and the expression quantity of IREB 2 is reduced. The cell apoptosis rate is reduced, and the Fe < 2 + > content and ROS level in cells are also reduced. After si-VDAC 1 is transfected, the change trend of si-VDAC 1 is similar to the trend after salidroside treatment. In addition, the treatment of an AKT inhibitor and an AKT activator shows that an AKT / GSK-3beta signal channel participates in the process of inducing the ferroptosis of the SH-SY 5 Y cells by the Abeta1-42.

The application provides a salidroside derivative, a preparation method and application thereof. The structure of the salidroside derivative is shown in formula I: wherein R1 is selected from H and carbonyl; R2 is selected from H and X is selected from O and NH; m is an integer of 3-6, and n is 0 or an integer of 3-6. The salidroside derivative has excellent antitumor activity, and the activity is obviously improved compared with salidroside. The preparation method of the salidroside derivative has mild reaction conditions, uses low-toxicity reagents, and has easily-obtained raw materials and convenient post-treatment.