347 results about "Salidroside" patented technology

The invention discloses rhodiola rosea fermentation protoplasm cosmetic and a preparation method thereof. Efficient ingredients of the rhodiola rosea fermentation protoplasm cosmetic are supernate obtained by mixing powder of rhodiola rosea root, water and zymophyte to obtain an initial system, fermenting the initial system to obtain zymotic fluid and sterilizing and centrifuging the zymotic fluid. The zymophyte is saccharomycete. The rhodiola rosea root is subjected to whole plant fermentation by adopting the saccharomycete, all functional ingredients and activity of the plant are kept, and loss of active ingredients caused by an extracting method is avoided. Any organic reagents are not added during extraction, fermentation temperature and fermentation pH are mild, structure of plant active ingredients is not damaged, natural activity of the plant is kept, and chemical components of essence and the like are not added in the fermentation protoplasm so as to ensure the safety of products on the human body. The rhodiola rosea fermentation protoplasm cosmetic is rich in salidroside, arbutin and the like having activities of restraining tyrosinase activity and restraining melanin synthesis so as to have a relatively strong whitening function on skin and has a synergistic effect with the components in the fermentation filtrate simultaneously.

The invention discloses total triterpene extract and total phenol extract extracted from Chinese herbal medicine glossy privet fruit and a preparation method thereof. The total triterpene extract mainly contains oleanolic acid, ursolic acid, acetyl oleanolic acid, indicant compound and other derivatives with acetyl oleanolic acid as the mother nucleus. The total phenol extract mainly contains hydroxyl mandelic, 3, 4-dihydroxy phenylethyl alcohol, salidroside and indicant compound and other derivatives with salidroside as the mother nucleus. The glossy privet fruit total triterpene extract and total phenol extract can be prepared by any method of solvent distillation, solvent extraction, deposition, macroporous resin absorption, supercritical fluid extraction, column chromatography and liquid-liquid countercurrent distribution chromatography, or any combination of these methods. The total percentage of various triterpenoids of the prepared glossy privet fruit total triterpene extract is 5 to 100 percent (w / w); wherein, the content of oleanolic acid and ursolic acid accounts for 5 to 100 percent (w / w) of the total triterpene. The total percentage of various phenols of the prepared glossy privet fruit total phenol extract is 5 to 100 percent (w / w); wherein, the content of salidroside accounts for 5 to 100 percent (w / w) of the total phenol.

The invention relates to an escherichia coli expression strain for high production of tyrosol and / or salidroside and icarisid D2 and an application of the escherichia coli expression strain, wherein the escherichia coli expression strain does not express feaB, tyrR, pykA, pykF and pheA genes, and contains and can express a tyrA* gene shown by SEQ ID No:1, an aroG* gene shown by SEQ ID No:2, and ppsA, tktA, aroB, aroD, aroE and ARO10 genes. The invention also provides a method for biologically synthesizing tyrosol and / or salidroside and icarisid D2 in the escherichia coli expression strain, wherein the yield of tyrosol can reach 600mg / L, the yield of salidroside can reach 50mg / L, and the yield of icarisid D2 can reach 40mg / L, so that the method is a new biological synthesis path for high production of tyrosol and / or salidroside and icarisid D2, lays a foundation for large-scale industrial production of tyrosol and / or salidroside and icarisid D2, and has important economic values and social benefits.

The invention relates to a method for extracting salidroside, polysaccharose and tannin-type substances from rhodiola root crude drugs. The method comprises the following steps of: firstly, drying and crashing rootstalks of the rhodiola root crude drugs; then, adopting the supercritical carbon dioxide technology to extract oil type substances from the rhodiola root crude drugs, and carrying out microwave extraction, deposition in alcohol and filtration so as to obtain sediment and supernatant fluid; and then, drying the sediment to obtain a rough polysaccharose powder product of the rhodiola, and carrying out absorption by macroporous resin, elution and drying to the supernatant fluid so as to obtain a salidroside powder product; and finally, using acetone for extracting microwave extraction residue, and obtaining the rhodiola root tannin extract after centrifugation and drying. The method can simultaneously extract three active ingredients, namely, salidroside, polysaccharose and tannin in the rhodiola root, thus improving the utilization rate of the rhodiola root and increasing the utilization value of the rhodiola root.

The invention discloses a production method for separation and purification of salidroside from rhodiola rosea. The method comprises the following steps: after being smashed, raw materials are thrown in from a throwing hopper; a mainframe of an extraction tank set rotates; the materials are slowly propelled from the front end of the set to the back end; and at the same time, extraction solvent is introduced in the extraction tank from a liquid inlet pipe at the tail end of the set, and penetrates the movable materials from the back end of the tank to flow to the front end; and the solid-phase and liquid-phase materials are fully contacted with each other in the inverse movement so as to extract the effective components of medicinal materials. Medicine dregs are forcedly pushed to a dreg outlet through a discharge conveyer for discharging, and are extruded by using a special juice extruder to extrude residual medicine juice from medicinal material organizations, so that the content of the residual medicine juice of the medicine dregs is reduced, the extraction efficiency is higher, and the extraction is more thorough. The method chooses macroreticular resins and combines with the silica column chromatography to purify, so that better separation effect is achieved. Finished products of salidroside with the highest yield of 1% and the content above 95% can be obtained.

The invention discloses a multi-channel nerve repair conduit with a tissue induced function and a mold; chitosan-coated salidroside microspheres and composite type salidroside slow-release microspheres are prepared, the drug cumulative release amount is calculated, the composite type salidroside slow-release microspheres with different contents are mixed with I-type collagen, and salidroside slow-release microspheres / I-type collagen is obtained; a multi-aperture cylindrical nerve conduit core layer is prepared by using the mold, a nano fiber nerve conduit shell layer is prepared by a high-pressure electrostatic spinning technology, the core layer and the shell layer are nested, and thus the multi-channel nerve repair conduit with the tissue induced function is prepared. The shell layer of the conduit has a good role in exchanging with a tissue fluid, and has a function of guiding nerve growth; the core layer has the functions of guiding nerve fiber orientation growth, promoting stem cells to directionally differentiate to schwann cells and accelerating the nerve fiber growth and function recovery, effectively repairs peripheral nerve defects, has good degradation and biocompatibility, and meets the requirements of a tissue engineering scaffold material.

The invention discloses a method for chemically synthesizing natural product salidroside. In the method, lewis acid serving as a catalyst, tyrosol of acyl protected phenolic hydroxyl serving as a glycosylation receptor, and beta-D-5 acetyl glucose serving as glycosylation donor perform a glycosylation reaction to obtain 1-[2-(4-acyloxy phenyl) ethyl]-2,3,4,6-O-tetraacetyl-beta-D-glucopyranoside; and acyl protecting group is removed in an alkaline condition to obtain salidroside. Compared with the traditional method, the method has easily available raw materials, the glycosylation receptor, the glycosylation product and the final product can be prepared through recrystallization, column chromatography and other purification processes are eliminated, the reaction step is short, the preparation process is simple, the glycosylation reaction catalyst has low cost and is easily available, so that the preparation cost is remarkably reduced; and a heavy metal salt catalyst is not used in the reaction, so that the synthesis product is more suitable to be used as a raw material of medicaments and health-care products. The method is suitable for industrial production of salidroside.

The invention relates to a separating purification method of a natural rhodioloside, which particularly belongs to the technical field of plant extraction deep-processing and is characterized by comprising the steps that: natural rhodiola sachalinensis is taken as a material which is crashed, subjected to alcoholic solvent extraction, two-step macroporous resin absorption and desorption, absolute ethyl alcohol crystallization, crystal mush centrifugalization and absolute ethyl alcohol washing, and later dried in vacuum so as to obtain high purity natural rhodioloside product. The purity of the rhodioloside extracted by the method can be up to over 99 percent, the total recovery coefficient of the rhodioloside can be up to over 40 percent in the whole process; the method has simple and convenient separating purifying technique and large treatment amount, is applicable to industrializing production, can provide technical support for deep development and utilization of the rhodiola sachalinensis resources, and provide excellent natural materials for medicine and function food industrialization.

The invention provides a method for preparing salidroside, which comprises the following steps: 1) extraction: adding water into a Rhodiola crenulata (Hook. f. et Thoms.) H. Ohba medicinal material, performing microwave extraction, filtering, concentrating and obtaining concentrate; 2) purification: performing secondary alcohol precipitation of the concentrate obtained by the step 1) and obtaining filtrate; and 3) refining: loading the filtrate obtained by the step 2) on a macroporous resin, eluting, concentrating, drying and obtaining the salidroside. The preparation method of the invention has the advantages of high extraction efficiency, simple process, capability for industrial production and the like.

The invention discloses recombinant Escherichia coli producing salidroside, a construction method and applications thereof. The construction method comprises: (1) using Escherichia coli [delta]A as a starting strain, and carrying out gene knockdown or gene silencing to make the galE gene, the galT gene and the ugd gene on the Escherichia coli chromosome be not expressed to obtain an Escherichia coli strain BMGU; and (2) introducing genes such as ARO10, UGT73B6MK, pgm, galU and T7Polymerase to over-express the genes such as ARO10, UGT73B6MK, pgm, galU and T7Polymerase so as to achieve the heterologous synthesis of salidroside, such that the recombinant Escherichia coli producing salidroside is obtained. According to the present invention, the efficient UDP-glucosyltransferase mutant UGT73B6MK is introduced, and the glucose metabolism pathway is optimized, such that the yield of salidroside is significantly improved.

The invention relates to a freezing liquid for human stem cells and a freezing storage method. Every 1000 ml of the freezing liquid comprises the following components: 20-50 mg of salidroside, 50-100 g of hydroxyethyl modified starch 450 Kd, 10-50 g of carboxylated poly-L-lysine, 50-100 g of sucrose, 20-150 g of trehalose, 5-20 g of antifreeze protein, 5-20 g of glycoprotein, 20-50 ml of DMSO, 60-100 ml of glycerin, and 200-300 ml of fetal calf serum, with the balance being a buffer solution. The freezing liquid for human stem cells is low in dimethyl sulfoxide content, has no side or toxic effect on frozen cells, can effectively keep the activity of stem cells, prevents oxidative injury, and is high in cell thawing rate.

The invention relates to a method for synthesizing salidroside by utilizing enzyme catalyzed direct glucosylation, comprising the following steps: carrying out a reaction on glucose, tyrosol and enzyme in a solvent containing a buffer solution; and collecting the salidroside by adopting the conventional method, wherein, the solvent comprises an icon-containing liquid and an organic solvent. Compared with the existing method, in the method, a reaction medium composed of the ionic liquid is adopted as a reaction system for synthesizing the salidroside by utilizing the enzyme catalyzed direct glucosylation; the enzyme activity and the enzyme stability are ensured; the dissolubility of the substrate in the reaction system is improved; the water activity in the reaction system is reduced; the expensive glucoside donor needs not to be used, and the salidroside can be further synthesized; the separation and purification processes of the product are simple, the unreacted tyrosol can be recovered and recycled, thus lowering the production cost, and satisfying the requirements of rapidly developing the medical industries.

The present invention discloses a method using Agrobacterium rhizogenes (Ar) gene to transform Rhodiola sachalinensis A.Bor to achieve a hairy root system for Salidroside production. By preparation of explant and cultivation in Ar bacteria liquid, Rhodiola sachalinensis A.Bor is induced to grow hairy roots by genetic transformation; the PCR testing proves that hairy roots are generated by transformation; a fermenter is used for massive production of hairy roots, and precursor substances and elicitors are added to improve the Salidroside content. The method is applied to produce Salidroside to substitute the drying-up Rhodiola sachalinensis A.Bor resources.

The invention discloses a method for catalyzing and synthesizing salidroside or analogues by utilizing glucose glycosyl transferase, comprising the following steps: carrying out a reaction on the leuconostoc mesenteroides glucose glycosyl transferase, cane sugar and alcohol in the water containing a buffer solution; and separating and collecting to obtain the salidroside or an analogue product. The enzyme catalytic reaction process disclosed by the invention is finished in a water phase and higher yield, is convenient and simple in operation; the product is easy to separate and purify, and has low environmental pollution and lower production cost; and the glucoside purity is higher after purification; and the process has large industrial application potential, and can satisfy the industrial requirements, such as medicaments and spices and the like.

The invention provides a rhodiola crenulata extract which is characterized in that: in the extract, the weight percentage of rhodioloside is not less than 10%, the weight percentage of rhodiola polysaccharide is not less than 30%, and the weight percentage of rhodiola polyphenol is not less than 34.0%; and the content of rosavin is 0%. The invention also provides a preparation method of the extract. The rhodiola crenulata provided by the invention has the advantages of exact drug effect, stable quality and strong controllability.

The invention discloses a health wine manufactured by taking caterpillar fungus or Chinese caterpillar fungus as the main materials as well as a preparation method. The health wine is manufactured by mixing the extraction of Chinese caterpillar fungus or caterpillar fungus, red common stonecrop herb and medlar with wine; the main active components of the health wine are caterpillar fungus element, caterpillar fungus acid, caterpillar fungus adenosine, salidroside and the amylase in the wine. The health wine has a comfort taste, can relieve asthma and eliminate phlegm, ease rheumatic diseases, resist fatigue, enhance memory, strengthen the body, delay senescence, prevent cardiovascular diseases, have remarkable adjusting function on the declining of sexual function, improve the concentration of the drug effect components by utilizing a multi-pot bidirectional reflux extraction technology without concentrating; the technique is more reasonable, thus not only saving the energies, but also ensuring the stability of the content of the drug effect components in the health wine, thereby ensuring the quality of the product and being applicable for scale production.

The present invention relates to a method for preparing rhodiola glucoside crude product by using glassy privet fruit as raw material and adopting macroporous adsorption resin purification technique. Said method includes the following steps: using 60%-80% ethyl alcohol to soak broken glossy privet fruit to obtain rhodiola glucoside extract, then making the obtained rhodiola glucoside extract undergo the processes of low-polar AB-8 type macroporous resin adsorption, washing with pure clean water, 25-35% ethyl alcohol elution, recovering ethyl alcohol and concentration so as to obtain the rhodiola glucoside crude product.

The invention discloses an ultrasonic extraction technology for salidroside in natural Rhodiola rosea, and relates to the technical field of traditional Chinese medicine extraction. The ultrasonic extraction technology comprises the following steps: firstly, slicing salidroside; freezing, baking, freezing again, and baking again; grinding to obtain powder; adding salidroside powder into purified water at the temperature of 40-60DEG C; adding 10-15 times of water for dipping for 30 minutes; acting for 5-10min at the temperature of 50-55DEG C under the condition of ultrasonic power of 440W / L; carrying out ultrasonic extraction for three times, and combining filtrate; filtering and condensing filtrate; adding 95% alcohol of certain volume; standing for 24 hours; removing supernate; carrying out reduced pressure suction filtration; carrying out vacuum drying at the temperature of 70DEG C; drying to obtain polygahatous polysaccharide. The ultrasonic extraction technology disclosed by the invention has the advantages of short extraction time, low energy consumption, big medicinal material raw material handling capacity which is improved twofoldness or by multiple times, few impurities, low cost and obvious overall economic efficiency, and efficient ingredients can be easily separated and purified.

The invention discloses an extract of a tyrosol plant, which is extracted from a root or a rhizome of a rhodiola rosea or from a mature fruit or a flower of a glossy privet fruit or from fermented soy sauce or vinegar residue. The invention also discloses an extract preparation of the tyrosol plant and the purpose of the extract preparation. The invention also discloses an extract of a salidroside plant of the tyrosol side product and the preparation and purpose of the extract. The extraction technology of the extracts of the invention is advanced, and the content of the effective components of the extracts is high, and the extracts can be fit for the requirements of the industrialized production technology and the pharmacy.

The invention discloses glycosyl transferase for catalyzing synthesis of gastrodin or salidroside, a gene coding the glycosyl transferase and application of glycosyl transferase. The glycosyl transferase for catalyzing synthesis of gastrodin or salidroside is as shown in SEQ ID No.1. Experiments prove that the glycosyl transferase for catalyzing synthesis of gastrodin or salidroside can be used for respectively catalytically converting hydroxy-benzyl alcohol into gastrodin and converting tyrosol into salidroside, and the yield of gastrodin and salidroside can be remarkably improved.

The invention provides a salidroside segmented copolymer lipid nanoparticle preparation for intravenous injection and a preparation method of the nanoparticle preparation, as well as application of the preparation in preparing anti-cancer medicaments. The preparation comprises salidroside, segmented copolymer, lipid and surfactant.

The invention relates to a method for separating high-purity solidroside from red common stonecrop herb, belonging to Chinese traditional medicine preparation field. The method comprises following steps: extracting raw material with hot water or water containing no more than 50% of methanol or ethanol; condensing the extrant and presipitaing it with ethanol; eluting with water solution containing no more than 10% of methanol or ethanol through chromatographic column of polymeric packing stationary phase; separating the product with chromatographic column of bonded stationary phase silica gel packing material and getting high-purity solidroside. The invention is characterized by large produtino scale, high production rate, high purity, repeated use of polymeric packing stationary phase and bonded silica gel packing stationary phase, and no volatile, toxic nd polluting containing organic solvent.

The invention provides a method for preparing salidroside with an enzymatic method. The method includes the specific steps that deep-eutectic solvents (DESs) synthesized by heating and stirring choline chloride and glycerin serve as reaction solvents, and the reaction solvents are biologically catalyzed and glycosylated into salidroside through beta-D-glucosidase, wherein the conversion rate of a substrate can be 30% or above (by glucose). According to the method, the adopted DESs are green and nontoxic, the solubility of the reaction substrate is high, and good biocompatibility is achieved. The preparing method has the advantages of being easy to operate, efficient, environmentally friendly, mild in condition, low in cost and the like.

The invention discloses a method for synchronously separating and preparing salidroside and rosavin from rhodiola rosea, comprising the following steps: (1) taking the rhodiola rosea as a raw material, and extracting by using analcohol aqueous solution containing a deep-eutectic solvent, wherein the deep-eutectic solvent has the effects of emulsifying, wetting, being assistant in dissolving and the like, and is beneficial for the extraction of the salidroside and the rosavin; (2) preparing a salidrosidecrude extracting solution and a rosavin crude extracting solution respectively from the extract by combining a mixed macroporous resinexpanded bed technology and a gradient elution technology; (3) preparing a salidrosideextract and a rosavin extract from the salidrosidecrude extracting solution and the rosavin crude extracting solution respectively by using the mixed macroporous resinexpanded bed technology again. Through HPLC content testing, the content of the salidroside in the salidroside extract is 40 percent to 80 percent, and the recovery is 60 percent to 88 percent; the content of the rosavin in the rosavinextract is 30 percent to 70 percent, and the recovery is 65 percent to 85 percent.

The invention discloses a separation method of salidroside impurities which includes the steps of first separation with silica gel column chromatography, elution with chloroform-ethanol gradients, collection by four sections and the separation and purification with semi-preparative high-performance liquid chromatography; wherein, the semi-preparative high-performance liquid chromatography is provided with the conditions as follows: a chromatographic column: ODS C18, 5mum and 250 * 10.0mm (I. D.); volume ratios of components of mobile phases: methanol to water equals to 11 to 89; flow rate: 3.0 mL / min; column temperature: 25 DEG C; detection wavelength: UV275nm; sample size: 50 muL; effluent liquid collection by section. The high-performance liquid chromatography of the salidroside and impurities thereof adopt the reversed-phase high-performance liquid chromatography with the following chromatographic conditions: a chromatographic column: ODS C18, 5mum and 150 * 4.6mm (I. D.); mobile phases: methanol to water equals to 15 to 85; flow rate: 1.0 mL / min; column temperature: 25 DEG C; detection wavelength: UV275nm; sample size: 20 muL. The contents of the salidroside and the impurities HEPG, HPAG and PAPG of the salidroside samples can be determined accurately and simultaneously by adopting the high performance liquid chromatography of the salidroside and impurities thereof, which provides a simple and reliable method for the quality control analysis in the production process.

The invention provides a cultivation method and plant application of a plant including salidroside. Tyrosol is added to plant growth solid mediums such as soil, ground substance and fertilizer, 0.1-100g tyrosol is added for one kilogram of the solid mediums or 0.1-1000g tyrosol is added for every plant, and the tyrosol is supplemented at certain time interval or not. The tyrosol taken by the plant is converted into salidroside under the action of related enzymes in cells, so that the plant can obtain physiological and pharmacological activity of the salidroside. The plant can be used directly and can be made into health food, drug, food additives and the like or can be used as a raw material for extracting the salidroside.

The invention discloses a making method of rhodiola root glycoside,which comprises the following steps: a. adding water to dissolve crude extract of rhodiola root; adjusting pH value as alkaline; filtering to obtain the filtrate; b. adjusting the filtrate to acid; adsorbing through adsorbing resin; eluting; condensing the eluent; sedimenting the eluent in the alcohol; filtering to obtain the filtrate; c. drying the filtrate through adsorbing resin; adding the dried material into column chromatograph; collecting the chromatographic liquor; d. condensing the chromatographic liquor; doing alcohol sediment; crystallizing. The invention also provides freeze dried and injection of the rhodiola root glycoside with simply and controllably operating condition and high safety, which is easy to accomplish for large scale of industrial manufacturing.

The invention discloses recombinant bacteria for producing salidroside and analogue thereof, and a construction method and use. The construction method comprises the steps of synthesizing keto-decarboxylase gene synkdc and glycosyl transferase gene synyjic, and connecting to a vector pRSFDuet-1 to construct a recombinant vector pSynkdc1-yjic; enabling T7RNA polymerase gene to be free or integrated to SyBE-002447 base bacterial strain, and introducing pSynkdc1-yjic to obtain SyBE-218011 and SyBE-218013 bacterial stains; and directly integrating synkdc and synyjic to SyBE-002447 chromosome, and removing feaB and ushA in a knockout manner to obtain a SyBE-218015 bacterial strain. The bacterial strain disclosed in the invention can be used for producing salidroside and analogue thereof through fermentation, so that the problem existing in source of the salidroside and analogue thereof can be solved, and cost can be lowered.

The invention belongs to the field of rhodiola sachalinensis cultivation and provides a method for cultivation of an improved variety of rhodiola sachalinensis in a low-altitude plain area. The method aims to solve the technical problems that in the process of cultivation of the rhodiola sachalinensis, root rot is prone to occurrence, the rate of emergence of seeds is low, and the yield is low. According to the method for cultivation of the improved variety of the rhodiola sachalinensis in the low-altitude plain area, a production cycle lasts for four years, a sowing plate is used for sowing, a bed surface is covered with pine needles and a mulching film, a shading net is erected on a bed, and weeding is conducted in time; in the next year, field planting is conducted, and organic compound fertilizer and farmyard manure which is processed through ferment bacteria are applied additionally; in the third year, after the seeds are harvested, stems and leaves above the ground are cut off, and field cultivation, vegetative propagation and other measures are taken; in the fourth year, fertilization is conducted from early May, and other aspects are managed according to the third year. By means of the method, the rate of emergence is over 90.5% which is more than three times that of common seeds which are sown, the morbidity of root rot is below 0.01%, the purity of the seeds is up to 100%, and the content of salidroside is up to 0.88%.