Method for producing salidroside and injection containing the same

A technology of salidroside and rhodiola, which is applied in the field of preparation of salidroside, can solve problems such as difficulty in obtaining high-purity salidroside, unfavorable industrialized large-scale production, and low yield of salidroside. Achieve the effects of being conducive to industrialized large-scale production, conducive to recycling, and easy to operate

Active Publication Date: 2008-02-27
成都诺迪康生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the preparation method, the fine extract of salidroside is adjusted to a pH of 1 to 5, and flowed through the adsorption resin column at normal temperature, the conversion rate is difficult, the adsorption rate is low, the yield of the prepared salidroside is low, and the implementability is poor. It is difficult to obtain high-purity salidroside, which is not conducive to the requirements of large-scale industrial production

Method used

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  • Method for producing salidroside and injection containing the same

Examples

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Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 The preparation method of salidroside of the present invention

[0031] Add the crude extract of Rhodiola rosea to water at 50°C, stir to dissolve, adjust the pH value to 11, filter to obtain a clarified filtrate, adjust the filtrate to pH value = 2, use HZ-816 adsorption resin for adsorption, and then wash with 10% ethanol Remove, concentrate the eluent completely to dryness, add absolute ethanol to precipitate, filter to obtain a clear filtrate, add the filtrate to the HZ-816 resin to absorb completely and then dry, and the HZ-816 adsorption resin after drying is added to the chromatographic column ( called the sample column), and eluted with 5% ethanol to obtain the eluate. Put the eluate directly on another chromatographic column (called a chromatographic column) for chromatography, concentrate the chromatographic liquid to dryness, add absolute ethanol to dissolve, filter, concentrate the filtrate until crystals are precipitated, and place it, Crystal...

Embodiment 2

[0032] Embodiment 2 The preparation method of salidroside of the present invention

[0033] Add the crude extract of Rhodiola rosea to water at 60°C, stir to dissolve, adjust the pH value to 12, filter to obtain a clarified filtrate, adjust the filtrate to a pH value of 3, use HZ-816 adsorption resin for adsorption, and then wash with 50% ethanol Remove, concentrate the eluent completely to dryness, add absolute ethanol to precipitate, filter to obtain a clear filtrate, add the filtrate to the HZ-816 resin to absorb completely and then dry, and the HZ-816 adsorption resin after drying is added to the chromatographic column ( called the sample column), and eluted with 35% ethanol to obtain the eluate. Put the eluate directly on another chromatographic column (called a chromatographic column) for chromatography, concentrate the chromatographic liquid to dryness, add absolute ethanol to dissolve, filter, concentrate the filtrate until crystals are precipitated, and place it, Cry...

Embodiment 3

[0034] Embodiment 3 The preparation method of salidroside of the present invention

[0035]Add the crude extract of Rhodiola rosea to water at 55°C, stir to dissolve, adjust the pH value to 8, filter to obtain a clear filtrate, adjust the filtrate pH value to 1, use HZ-816 adsorption resin for adsorption, and then wash with 20% ethanol Remove, concentrate the eluent completely to dryness, add absolute ethanol to precipitate, filter to obtain a clear filtrate, add the filtrate to the HZ-816 resin to absorb completely and then dry, and the HZ-816 adsorption resin after drying is added to the chromatographic column ( called the sample column), and eluted with 5% ethanol to obtain the eluate. Put the eluate directly on another chromatographic column (called a chromatographic column) for chromatography, concentrate the chromatographic liquid to dryness, add absolute ethanol to dissolve, filter, concentrate the filtrate until crystals are precipitated, and place it, Crystallize and...

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Abstract

The invention discloses a making method of rhodiola root glycoside,which comprises the following steps: a. adding water to dissolve crude extract of rhodiola root; adjusting pH value as alkaline; filtering to obtain the filtrate; b. adjusting the filtrate to acid; adsorbing through adsorbing resin; eluting; condensing the eluent; sedimenting the eluent in the alcohol; filtering to obtain the filtrate; c. drying the filtrate through adsorbing resin; adding the dried material into column chromatograph; collecting the chromatographic liquor; d. condensing the chromatographic liquor; doing alcohol sediment; crystallizing. The invention also provides freeze dried and injection of the rhodiola root glycoside with simply and controllably operating condition and high safety, which is easy to accomplish for large scale of industrial manufacturing.

Description

technical field [0001] The invention provides a preparation method of salidroside, which belongs to the field of medicine. Background technique [0002] Rhodiola is the root and rhizome of the genus Rhodiola (Rhodiola L.) in the sedum family. There are about 100 species of Rhodiola plants in the world, which can grow in extremely harsh and changeable natural environments. An adaptogenic plant found mainly in the Himalayas, NW Asia and North America. There are about 80 species in my country, mostly distributed in the southwest, northwest, north and northeast regions. Current studies have found that Rhodiola contains many active ingredients such as glycosides, polysaccharides, organic acids, flavonoids, volatile oils, proteins, trace elements, etc., and salidroside is one of the important active ingredients. , has biological functions such as anti-hypoxia, anti-fatigue, anti-aging, anti-radiation, anti-tumor, lowering blood fat and preventing and treating cardiovascular dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18A61K31/7028A61K9/19A61K9/08A61P9/00
Inventor 张春容董兆勇彭红卫杨伟赵斌曾裕建张宝华康海歧李好雨陈春会
Owner 成都诺迪康生物制药有限公司
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