303results about How to "Large amount of preparation" patented technology

The invention relates to a Chinese magnoliavine fruit monomer composition separation preparation method; ethanol extracts from Chinese magnoliavine fruit are extracted by petroleum ether, chromatography is carried out to the petroleum ether extracts by a silicagel column, and then the petroleum ether is eluted by petroleum ether-ethylacetate, and HPLC is used for monitoring, and then crude extract A and crude extract B are fraction-collected, and eluted; the volume ratio of eluant petroleum ether and ethylacetate is 3-5:1, the crude extract A and crude extract B are respectively applied to a high-speed counter-current chromatography for separation, so as to obtain SCHisanhenol, deoxyschizandrin, schizandrin B, schisandrin C, schizandrol A, schizandrol B and schisantherrin B monomers, and the purity is higher than 98 percent.

The invention pertains to the field of extracting active ingredients of natural products and particularly relates to an extracting method and a purifying method of crude phellinus igniarius polysaccharides. The technical problems needed to be solved are to disclose a method for extracting the extract of crude phellinus igniarius polysaccharides and an efficient method for preparing phellinus igniarius polysaccharides through countercurrent chromatography. An ultrasonic extraction is adopted by the invention to extract crude phellinus igniarius polysaccharides from phellinus igniarius fruit bodies; the high-speed countercurrent chromatography is adopted to further purify the crude phellinus igniarius polysaccharides. The extracting method and the purifying method of the invention have high yield of crude phellinus igniarius polysaccharides, and can take up 6 percent of crude drugs; the purity of the further purified phellinus igniarius polysaccharides is greatly improved to be about 75 percent.

A process for preparing globeflower flavone, luxtexin, vitexin and quercetin-neohesperidoside from Chinese globeflower flower includes such steps as reflux extract in alcohol solution, adsorbing by macroreticular resin, cluting, concentrating, drying to obtain globeflower flavone, and separating and purifying, luxtexin, vitexin and quercetin-neohesperioside by HSCCC.

The invention discloses a method for preparing a high-purity taste-removing purple sweet potato pigment, and belongs to the field of food additives and agricultural product deep processing. The method for preparing the high-purity taste-removing purple sweet potato pigment comprises the steps that 1, pretreatment is conducted on purple sweet potato raw materials; 2, the purple sweet potato pigment is extracted from low-frequency microwave auxiliary acidified aqueous solution; 3, preliminary purification is conducted on macroporous resins; 4, high-speed counter-current chromatography separation and purification are conducted, and the taste-removing purple sweet potato pigment with concentration more than 95% is obtained. According to the method for preparing the high-purity taste-removing purple sweet potato pigment, the microwave auxiliary acidified aqueous solution is used for extraction, flocculants are added to flocculate mucoproteins, then macroporous resin static adsorption and dynamic elution are conducted, and the taste-removing and enrichment purification process is preliminarily accomplished, and the high-purity taste-removing purple sweet potato pigment is finally prepared through the high speed counter-current chromatography method in a separation mode. According to the method for preparing the high-purity taste-removing purple sweet potato pigment, technological processes are advanced, separation efficiency is high, recovery rate is high, product purity is more than 95%, preparation amount is large, an obtained product is free of peculiar smell, has few impurities and is good in solubility, and method for preparing the high-purity taste-removing purple sweet potato pigment is easy to popularize and use.

The invention relates to a chemically mechanical polishing solution for a silicon carbide substrate. The chemically mechanism polishing solution comprises the following components in percentage by weight: 1-50 percent of grinding material, 0.01-8 percent of chelant, 0.01-10 percent of surfactant, 0.01-10 percent of dispersing agent, 0.1-20 percent of oxidant and the balance of deionized water. The polishing solution has little damage to the surface of the silicon carbide substrate; the silicon carbide substrate has low roughness degree value and can reach the roughness Ra of being less than 0.5nm; no scratch and corrosion pit defect exists on the surface of the silicon carbide substrate; and the chemically mechanical polishing solution has high removing speed of scratch and corrosion pit, is easy to clean, does not corrode processing equipment and pollute the environment, and is low is price of raw materials, low in cost and easy to store. The chemically mechanical polishing solution is mainly used for chemically mechanical polishing of a silicon carbide wafer for the substrate.

The invention relates to the technical field of medicines and discloses a method for separating and purifying impurities in a sodium tanshinone IIA sulfonate crude drug. Sodium tanshinone IIA sulfonate is widely used for treating cardiovascular and cerebrovascular diseases and other systemic disease at present. 1, 2-dehydrogenation sodium tanshinone IIA sulfonate, sodium tanshinone IIB sulfonate and 1-hydroxy sodium tanshinone IIA sulfonate are three major impurities in the sodium tanshinone IIA sulfonate crude drug. The method for separating and purifying the impurities in the sodium tanshinone IIA sulfonate crude drug is that a high-speed counter-current chromatography method is used for separating and purifying the three major impurities in the sodium tanshinone IIA sulfonate crude drug. The method has the advantages of being simple, fast, high in recovery rate, large in preparation quantity, high in separation efficiency, good in product purity, convenient and suitable for studying comparison products, and defects of cumbersome operations and long separation periods of traditional preparation methods are overcome.

The invention discloses a method for preparing a porous covalent organic material by using the ultra-gravity method, and belongs to the technical field of preparation of porous covalent organic materials. The method comprises the following steps: under a high gravity field, the micromixing and mass transferring process is strengthened, materials in a cavity of a preferable over-gravity rotary bed are sucked into a rotor of the rotary bed through a rotating inner spiral pipe, and the materials are sheared by, mixed and reacted with a filler rotating at a high speed in the rotor. Compared with reaction under a normal gravity filed, the method is greatly increased in reaction rate, and shortened in reaction time; besides, as for reaction systems sensitive to air, the water-free and oxygen-free reaction condition can also be achieved without a glove box, and compared with low-dosage preparation in the glove box, the method is greater in preparation amount and higher in production efficiency.

The invention discloses a method for separating active ingredients from an aquilaria sinensis lamina by utilizing a high-speed counter current chromatography. The method comprises the following steps of: extracting the dried and smashed aquilaria sinensis lamina by virtue of a percolation extract method at the room temperature to obtain an aquilaria sinensis lamina extracting solution extractum; extracting the extracting solution extractum for 3-4 times by sequentially utilizing petroleum ether,and ethyl acetate, respectively decompressing and concentrating a petroleum ether extract liquor and an ethyl acetate extract liquor to extractums; then respectively separating the petroleum ether part extractum and the ethyl acetate part extractum to obtain an active ingredient A of 7,4'-dimethoxy-5-hydroxy flavone by utilizing the high-speed counter current chromatography; and then separating the ethyl acetate part extractum through high-speed countercurrent chromatography to obtain an active ingredient B of 3,5,7,3',4'-pentahydroxy-flavone and an active ingredient C of 3,5,7,4'-tetrahydroxy-flavone after coarsely separating the ethyl acetate part extractum by virtue of a column chromatography on silica gel. The active ingredients separated by the method are low in loss, high in separation speed, high in purity, stable and easy to apply.

The invention discloses a making method of rhodiola root glycoside,which comprises the following steps: a. adding water to dissolve crude extract of rhodiola root; adjusting pH value as alkaline; filtering to obtain the filtrate; b. adjusting the filtrate to acid; adsorbing through adsorbing resin; eluting; condensing the eluent; sedimenting the eluent in the alcohol; filtering to obtain the filtrate; c. drying the filtrate through adsorbing resin; adding the dried material into column chromatograph; collecting the chromatographic liquor; d. condensing the chromatographic liquor; doing alcohol sediment; crystallizing. The invention also provides freeze dried and injection of the rhodiola root glycoside with simply and controllably operating condition and high safety, which is easy to accomplish for large scale of industrial manufacturing.

The invention discloses a method for separating and purifying mangiferin from aquilaria sinensis leaves. The method is used for solving the problems such as low purity, low yield and complex operation of the mangiferin separated by virtue of the existing process. The method comprises the following step: quickly preparing the high-purity mangiferin by use of the aquilaria sinensis leaves used as raw materials by virtue of adopting a method of combining normal phase column chromatography with high-speed counter-current chromatography. The method has the advantages of great preparation amount, high efficiency, quickness, low cost, simplicity in operation, high product quality and no pollution; and moreover, a solvent can be recycled, the solvent cost and the solvent treatment cost can be lowered, and the method can be widely applied to industrial production.

The invention relates to a process for extracting buddleoside, which comprises the following steps of: adding water or an organic solvent in an amount which is 5 to 12 times the mass of residue of wild chrysanthemum and extracting for 1 to 5 times, wherein the residue is obtained after volatile oil is extracted from the wild chrysanthemum and is used as a raw material; combining extract, filtering, filtering filtrate by using a filter membrane, and performing chromatographic separation and purification on the filtrate by using a packed column; and drying to obtain a product. Membrane separation technology is adopted for separation and purification, so that on the one hand, the purity of the buddleoside in the product is improved, and on the other hand, sample solution before column chromatography is purified, damage to fillers is relieved, and the life cycle of the column chromatography fillers is prolonged; in addition, nanofiltration technology is applied, so that the sample solution before the column chromatography does not need to be concentrated and can be directly loaded, and the process is simplified.

The invention discloses a method for preparing schaftoside from desmodium styracifolium. The method comprises the following steps of: i, crushing the desmodium styracifolium, adding biological enzyme and uniformly stirring, performing enzymolysis at normal temperature for 4-7 days, soaking enzymolysis raw material with warm water and extracting for two to three times, filtering extract and ultra-filtering with an ultrafiltration membrane, concentrating permeate with a nanofiltration membrane, extracting the concentrate by using acetic ether and concentrating the extract to obtain a rough extract; and ii, separating the rough extract by using high-speed counter-current chromatography, performing on-line monitoring through an ultraviolet detector, collecting a target component and performing reduced pressure drying to obtain the schaftoside. The method has the advantages of simple operation, high efficiency, large preparation capacity and high purity of an obtained product.

The invention discloses a preparation method of demethylwedelolactone, which is simple and convenient to operate and small in pollution. The method comprises the following steps of: crushing an eclipta raw material; adding 5-10 times of 40-70% methanol liquor for ultrasonic extraction for 2-3 times; concentrating the extracting liquor and extracting by ethyl acetate liquor; collecting the extract recovering reagent, adding macroporous resin to adsorb, and eluting by 5-10 times of 50% methanol liquor in volume; concentrating the eluent to obtain a crude extract; and then, separating by high speed counter current chromatography, online monitoring by an ultraviolet detector and collecting the fraction and decompressing and drying to obtain demethylwedelolactone. According to the method for preparing demethylwedelolactone, the purity of the product obtained is high, and the method is beneficial for industrial amplification.

The invention discloses a nitrogen and phosphorus-containing hydrotalcite-based flame retardant, which comprises the following raw materials: hydrotalcite, a nitrogen-containing monomer, sodium hydroxide and a phosphorus-containing monomer. The preparation method comprises the following steps: (1) weighing the raw materials; (2) ultrasonically dispersing hydrotalcite and a nitrogen-containing monomer in deionized water for later use; (3) ultrasonically dispersing sodium hydroxide and a phosphorus-containing monomer into deionized water; and (4) dropwise adding the phosphorus-containing monomer alkali liquor into the hydrotalcite suspension under the condition of magnetic stirring, and carrying out heating reaction, then performing filtration, vacuum drying and grinding to obtain the phosphorus-containing hydrotalcite. The phosphorus-containing monomer and the nitrogen-containing monomer are introduced into a hydrotalcite system, so that the advantages of biomass and hydrotalcite can be combined; therefore, the organic-inorganic hybridized flame retardant is obtained and is applied to a polymer matrix, wherein the defect that the adding amount of hydrotalcite is large is overcome, and the flame retardant has important significance on polymer flame retardance.

The invention discloses a device and a method for preparing directionally arranged fiber by a solution blow-spinning technology. The device comprises a solution blow-spinning system and a system for collecting fiber directionally arranged by airflow; the solution blow-spinning system comprises a spinning solution, an airflow control system and a blow-spinning device; the blow-spinning device, a spinning solution and the airflow control system are connected; a spinning collection system comprises a paving collection net; the paving collection net is connected with a negative pressure device; and the solution blow-spinning system collects directionally arranged nanometer fiber on the paving collection net by adjusting the angle of the spinning air flow and the paving collection net and by taking high-speed airflow as a directional driving force. The device and the method are relatively simple to operate, have many adjustable parameters and are high in precision; and the fiber obtained through preparation is high in arrangement degree, large in preparation quantity and high in uniformity.

The invention relates to a preparation method of a 1,4-di-[4-(glucosyloxy) benzyl]-2-isobutyl malate comparison product. The method comprises the following steps of: pulverizing a rhizoma bletillae medicinal material into coarse powder, extracting in alcohol and recovering the alcohol; adding water to an obtained concentrated solution to dilute and enriching through a macroporous adsorption resincolumn; hydrolyzing and adsorbing in alcohol, and recovering the alcohol to obtain a concentrated solution; adding silica gel to the concentrated solution and uniformly mixing; drying to form powder and eluting by using chloroform and methanol to obtain a crude 1,4-di-[4-(glucosyloxy) benzyl]-2-isobutyl malate product; carrying out gel column chromatography to obtain a fine 1,4-di-[4-(glucosyloxy) benzyl]-2-isobutyl malate product; and then carrying out preparative reverse silica gel column chromatography and methanol-water elution to obtain the 1,4-di-[4-(glucosyloxy) benzyl]-2-isobutyl malate comparison product. Detected by using a high efficiency liquid chromatography method, the content of the 1,4-di-[4-(glucosyloxy) benzyl]-2-isobutyl malate can reach higher than 98 percent. The method has lower cost, simple step and strong specificity.

The invention relates to a method for preparing methylated catechin from tea, comprising following steps: tea powder is extracted with ethanol and petroleum ether to remove chlorophyll and fat-soluble constituents; methylated catechin crude extraction dry powder A is obtained through extraction with ethyl acetate; methylated catechin crude product is obtained via separation and purification with crude polyamide resin and fine polyamide resin; methylated catechin pure product is obtained via further purification with high speed counter current chromatography. The invention has the advantages that the invention establishes purification craft of methylated catechin firstly at home and obtains high purity methylated catechin; the invention adopts high speed counter current chromatography (HSCCC) preparation craft, which does not need solid support and overcomes defects such as sample adsorption, loss, pollution and tailed peak arisen from solid phase support compared to high speed liquid chromatography (HSLC) preparation craft; high speed counter current chromatography also has the advantages of large quantity preparation, low comprehensive cost and easy industrial popularization.