Process for preparing thermopsis lanceolate total alkaloids and several high-purity medicinal substances

Abstract

The invention relates to a process for preparing thermopsis lanceolate total alkaloids and several high-purity medicinal substances, and belongs to the technical field of the extraction of Chinese medicinal active ingredients. The medicinal substances comprise eulexine, thermopsine, sparteine and N-methyl eulexine. In the process, total alkaloids are prepared from thermopsis lanceolate serving as a raw material through acid water extraction, ionic resin adsorption and macroporous resin enrichment, and the total alkaloids are separated and purified by high-speed countercurrent chromatography and preparative liquid chromatography to obtain the high-purity eulexine, thermopsine, sparteine and N-methyl eulexine. The process has the advantages of simplicity, quickness, high repeatability, large preparation amount and the like, and industrialization is easy to realize.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of active components of traditional Chinese medicines, and in particular relates to a preparation process of total alkaloids of lanceolata chinensis and several high-purity medicinal substances. Background technique [0002] Coniferous yellow flower ( Thermopsis lanceolate ), alias Mu Madou, Mongolian "Furday Bagan-Xiri", is a perennial herb belonging to the genus Cassia genus Fabaceae. Distributed in the northeast, north and Shaanxi, Gansu, Qinghai, Ningxia, Sichuan and other provinces and regions, the main chemical components of lanceolus Huanghua are quinolizidine alkaloids, and the total alkaloid content varies greatly due to different parts. The content is the highest, followed by flowers, fruits, leaves, stems, and roots. This type of alkaloid has various pharmacological effects such as anti-cancer, anti-arrhythmia, anti-microbial, anti-ulcer, and increasing white blood ...

AI Technical Summary

Problems solved by technology

The method disclosed in Chinese Patent Application No. 200910023485.5 is to pulverize the seeds of lanceolus Huanghua, extract and concentrate to obtain the extract, extract the extract with an organic solvent, back-extract the acid aqueous solution, purify the extraction solvent, and simultaneously obtain the acid containing Huang Hua alkali. Aqueous solution, this acid aqueous solution is alkalized by adding alkaline reagents, crystallized, and recrystallized to obtain chrysanthemum; the extracted extract solution is alkalized and then extracted with an organic solvent, concentrated, crystallized, and recrystallized to obtain pure gorse Alkali, this method uses a large amount of acid-base solution, which is not conducive to the stability of the components, and uses strong toxic solvents such as benzene, which is unsafe to operate

The method disclosed in Chinese Patent Application No. 201010240158.8 is to obtain the total alkaloids through extraction, concentration, acid water acidification, alkalization, and organic solvent extraction. The total alkaloids are dissolved in water and extracted with organic solvents to obtain cytisine. The method Cytisine can be obtained only by extracting from total alkaloids with organic solvents, which is not easy to achieve in actual production