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6 results about "Countercurrent chromatography" patented technology
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Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid stationary phase that is held in place by centrifugal force and is used to separate, identify, and quantify the chemical components of a mixture. In its broadest sense, countercurrent chromatography encompasses a collection of related liquid chromatography techniques that employ two immiscible liquid phases without a solid support. The two liquid phases come in contact with each other as at least one phase is pumped through a column, a hollow tube or a series of chambers connected with channels, which contains both phases. The resulting dynamic mixing and settling action allows the components to be separated by their respective solubilities in the two phases. A wide variety of two-phase solvent systems consisting of at least two immiscible liquids may be employed to provide the proper selectivity for the desired separation.
Method for purifying melittin with high purity by using pH zone refining countercurrent chromatography
PendingCN122444848ACountercurrent chromatographyMellitoxin
The application relates to a high-purity melittin purification method using pH zone refining countercurrent chromatography. In order to overcome the defects of the prior art, a solvent system composed of 4-12% methyl tert-butyl ether, 42-50% n-butanol, 4-12% acetonitrile and 42-50% deionized water is used, a high-purity melittin solution is purified by using the pH zone refining countercurrent chromatography, and the finished high-purity melittin is obtained through concentration and drying. The application does not use solid fillers, does not cause loss to the sample, can almost recover all the melittin in the crude melittin, the yield is more than 90%, and the application is suitable for batch purification of high-purity melittin.
Owner:BEIJING 4DSTAR TECH
A method for extracting and separating high-purity natural nicotine from tobacco
This invention relates to a method for extracting and separating high-purity natural nicotine from tobacco. The invention utilizes high-speed countercurrent chromatography to prepare high-purity natural nicotine. By selecting a suitable solvent system, a system with an appropriate partition coefficient is obtained, significantly improving the separation and purification effect of nicotine. The process is simpler, more convenient to operate, results in no sample loss, is highly efficient and rapid, and allows for large-scale preparation with high purity. This invention also utilizes a eutectic solvent to extract natural nicotine from tobacco. This eutectic solvent is safe, low in toxicity, non-volatile, and reusable, making it a green and environmentally friendly solvent. The extraction method has high extraction efficiency, produces fewer impurities in the extract, and significantly reduces the amount of volatile organic solvents used, making it even more environmentally friendly.
Owner:GUANGDONG SHENNONG TOBACCO TECH CO LTD
A method for extracting and separating lycopene A and lycopene B from Lycium bark.
This invention provides a method for the simultaneous extraction and separation of lycopene A and lycopene B from Lycium chinense root bark. Based on obtaining an effective eutectic solvent as the extractant, ultrasonic-assisted extraction is employed. Response surface methodology is used to optimize three factors significantly affecting the extraction process of lycopene A and lycopene B: extraction time, extraction temperature, and water content, thus obtaining optimal extraction conditions. Subsequently, the Lycium chinense root bark extract is passed through an ion exchange resin to remove the eutectic solvent. Finally, high-speed countercurrent chromatography (HSCLC) separation and preparative liquid chromatography (PCLC) purification are used to further obtain lycopene A and lycopene B with high purity, which are then detected and analyzed using high-performance liquid chromatography (HPLC).
Owner:LANZHOU INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES +1
Method for preparing cannabidiol by seperation and purification using high-speed countercurrent chromatography
The present invention relates to a method for preparing cannabidiol by separation and purification using high-speed countercurrent chromatography, comprising: alcohol extraction and water precipitation, adsorption with a macroporous resin, and high-speed countercurrent chromatography separation. The present invention separates and obtains high-purity cannabidiol from industrial hemp flowers or leaves, while at the same time removing the psychotoxic component tetrahydrocannabinol by combining a microporous resin chromatographic column with a high-speed countercurrent chromatograph, and optimizing process parameters, and the solvent used therein being environmentally friendly, leaving no residues, having low cost and being recyclable. Therefore, the method is suitable for industrial production.
Owner:TECHSON IND CO LTD
A method for efficient enrichment and separation of isoflavones from kudzu root
This invention discloses a highly efficient method for the enrichment and separation of isoflavones from kudzu root, belonging to the field of traditional Chinese medicine extraction and separation technology. The method includes: adsorbing and enriching kudzu root juice using HP-20 macroporous adsorption resin, optimizing the sample loading and elution conditions, and eluting with 50% ethanol to obtain a crude isoflavone extract; then separating the crude extract using high-speed countercurrent chromatography, employing a solvent system of ethyl acetate-n-butanol-water with a volume ratio of 2:1:3 to achieve efficient and simultaneous separation of four isoflavone components: puerarin, 3′-methoxypuerarin, puerarin apigenin, and daidzein. This invention features a simple process, low solvent consumption, and high separation efficiency, simultaneously obtaining multiple high-purity isoflavone monomers, making it suitable for large-scale production.
Owner:NORTHWEST A & F UNIV
A method for extracting and separating five compounds from vaccinium uliginosum pomace
This invention relates to the field of separation and purification technology, and in particular to a method for extracting and separating five compounds from the pomace of red bean and blueberry. This invention develops a microwave-ultrasound synergistic enhanced extraction coupled with carbon dioxide gas-responsive in-situ eutectic solvent targeted extraction technology, combined with macroporous resin enrichment and high-speed countercurrent chromatography separation technology, achieving efficient extraction of five monomeric components: epicatechin, astragaloside, quercetin, kaempferol, and ursolic acid. This process significantly reduces time consumption, energy consumption, and environmental pollution, providing technical support for the large-scale industrial production of red bean and blueberry resources for high-value utilization.
Owner:ZHEJIANG PHARMA COLLEGE