10 results about "Countercurrent chromatography" patented technology

The application relates to a high-purity melittin purification method using pH zone refining countercurrent chromatography. In order to overcome the defects of the prior art, a solvent system composed of 4-12% methyl tert-butyl ether, 42-50% n-butanol, 4-12% acetonitrile and 42-50% deionized water is used, a high-purity melittin solution is purified by using the pH zone refining countercurrent chromatography, and the finished high-purity melittin is obtained through concentration and drying. The application does not use solid fillers, does not cause loss to the sample, can almost recover all the melittin in the crude melittin, the yield is more than 90%, and the application is suitable for batch purification of high-purity melittin.

This invention belongs to the field of traditional Chinese medicine pharmaceutical technology and relates to a method for preparing aristolochic acid lactam components from Houttuynia cordata. This invention establishes a method for rapidly enriching aristolochic acid lactam components from Houttuynia cordata using high-speed countercurrent chromatography. It enriches three lactam components—aristolochic acid lactam A II, aristolochic acid lactam FI, and aristolochic acid lactam B II—from Houttuynia cordata. The enrichment process of aristolochic acid lactam components in this invention is simple, rapid, and yields high amounts, making it suitable for industrial-scale preparation. It can be used for large-scale enrichment of aristolochic acid lactam compound reference standards in traditional Chinese medicine. Furthermore, it provides essential reference standards for the safety evaluation and quality control of Houttuynia cordata, a traditional Chinese medicine for clearing heat and detoxifying.

This invention relates to a method for extracting and separating high-purity natural nicotine from tobacco. The invention utilizes high-speed countercurrent chromatography to prepare high-purity natural nicotine. By selecting a suitable solvent system, a system with an appropriate partition coefficient is obtained, significantly improving the separation and purification effect of nicotine. The process is simpler, more convenient to operate, results in no sample loss, is highly efficient and rapid, and allows for large-scale preparation with high purity. This invention also utilizes a eutectic solvent to extract natural nicotine from tobacco. This eutectic solvent is safe, low in toxicity, non-volatile, and reusable, making it a green and environmentally friendly solvent. The extraction method has high extraction efficiency, produces fewer impurities in the extract, and significantly reduces the amount of volatile organic solvents used, making it even more environmentally friendly.

The invention relates to the technical field of counter-current chromatographs, in particular to a high-mass-transfer separation column of a single-rotation counter-current chromatograph, which comprises a separation column bottom plate arranged on a main shaft, the power amplification plate is hung on the separation column bottom plate and connected with the ultrasonic generator, and a spiral groove is formed in the power amplification plate; the spiral pipe is embedded in the spiral groove; the spiral pipe pressing plate is arranged above the spiral pipe; the separation column top plate is arranged above the spiral pipe pressing plate; and the switching assembly is arranged on the top plate of the separation column and is used for connecting an external feeding pipe with the inlet / outlet of the spiral pipe. A plane coil pipe structure formed by winding a single continuous pipeline is adopted to replace a traditional split type combined structure, and the risk of sealing leakage of a combined surface is eliminated; interphase mass transfer online dynamic regulation and control are realized through the ultrasonic generator, so that the separation efficiency is remarkably improved; the modular design simplifies the manufacturing process, and reduces the cost and maintenance difficulty.

This application relates to the field of natural product chemistry, specifically disclosing a method for extracting triptolide and its application. The extraction method for triptolide includes the following steps: S1, pretreatment: After washing and drying the roots of Tripterygium wilfordii, it is subjected to ultrafine pulverization and plasma treatment to obtain Tripterygium wilfordii powder; S2, enzymatic hydrolysis: After enzymatic hydrolysis of the Tripterygium wilfordii powder, the enzyme is inactivated, centrifuged, concentrated, and dried to obtain crude triptolide; S3, purification: The crude triptolide is purified by high-speed countercurrent chromatography and vacuum dried to obtain refined triptolide. The above extraction method is simple and safe to operate, and the purity and extraction rate of the obtained triptolide are improved. The obtained triptolide can be mixed with total glucosides of paeony, glycyrrhizic acid, papain, poloxamer, xanthan gum, and propylene glycol to prepare a drug for treating rheumatoid arthritis, which has significant efficacy, high safety, and broad clinical application prospects.

This invention provides a method for the simultaneous extraction and separation of lycopene A and lycopene B from Lycium chinense root bark. Based on obtaining an effective eutectic solvent as the extractant, ultrasonic-assisted extraction is employed. Response surface methodology is used to optimize three factors significantly affecting the extraction process of lycopene A and lycopene B: extraction time, extraction temperature, and water content, thus obtaining optimal extraction conditions. Subsequently, the Lycium chinense root bark extract is passed through an ion exchange resin to remove the eutectic solvent. Finally, high-speed countercurrent chromatography (HSCLC) separation and preparative liquid chromatography (PCLC) purification are used to further obtain lycopene A and lycopene B with high purity, which are then detected and analyzed using high-performance liquid chromatography (HPLC).

The present invention relates to a method for preparing cannabidiol by separation and purification using high-speed countercurrent chromatography, comprising: alcohol extraction and water precipitation, adsorption with a macroporous resin, and high-speed countercurrent chromatography separation. The present invention separates and obtains high-purity cannabidiol from industrial hemp flowers or leaves, while at the same time removing the psychotoxic component tetrahydrocannabinol by combining a microporous resin chromatographic column with a high-speed countercurrent chromatograph, and optimizing process parameters, and the solvent used therein being environmentally friendly, leaving no residues, having low cost and being recyclable. Therefore, the method is suitable for industrial production.

This invention discloses a highly efficient method for the enrichment and separation of isoflavones from kudzu root, belonging to the field of traditional Chinese medicine extraction and separation technology. The method includes: adsorbing and enriching kudzu root juice using HP-20 macroporous adsorption resin, optimizing the sample loading and elution conditions, and eluting with 50% ethanol to obtain a crude isoflavone extract; then separating the crude extract using high-speed countercurrent chromatography, employing a solvent system of ethyl acetate-n-butanol-water with a volume ratio of 2:1:3 to achieve efficient and simultaneous separation of four isoflavone components: puerarin, 3′-methoxypuerarin, puerarin apigenin, and daidzein. This invention features a simple process, low solvent consumption, and high separation efficiency, simultaneously obtaining multiple high-purity isoflavone monomers, making it suitable for large-scale production.

The invention relates to a method for preparing high-purity pinocembrin by high-speed counter-current chromatography separation, which comprises the following steps: taking propolis extract as a raw material, fully shaking a solvent system, standing for phase separation, and separately collecting an upper phase and a lower phase; the propolis extract is dissolved in an upper phase, the upper phase serves as a stationary phase, a lower phase serves as a mobile phase, high-speed counter-current chromatography is used for separation and purification, a mixed solution of the pinobufagin and the mobile phase is obtained, the mobile phase is removed, and the pinobufagin is obtained. According to the method, the high-speed counter-current chromatography technology is utilized for the first time to separate and purify the pinocembrin with the purity larger than 98% from the propolis, and the method has the advantages of no sample loss, no pollution, high efficiency, large-scale preparation, solvent recycling and the like; reagents of a solvent system adopted by the method can be recycled and reused, so that the method has a good environment-friendly function.

This invention relates to the field of separation and purification technology, and in particular to a method for extracting and separating five compounds from the pomace of red bean and blueberry. This invention develops a microwave-ultrasound synergistic enhanced extraction coupled with carbon dioxide gas-responsive in-situ eutectic solvent targeted extraction technology, combined with macroporous resin enrichment and high-speed countercurrent chromatography separation technology, achieving efficient extraction of five monomeric components: epicatechin, astragaloside, quercetin, kaempferol, and ursolic acid. This process significantly reduces time consumption, energy consumption, and environmental pollution, providing technical support for the large-scale industrial production of red bean and blueberry resources for high-value utilization.