12 results about "Sulbactam Sodium" patented technology

The invention discloses an analysis method for a genotoxic impurity (2S)-2-amino-3-ethoxysulfinyl-3-methylbutyric acid in a sulbactam sodium raw material. The analysis method has strong specificity and high sensitivity, and can be used for monitoring the target product of the synthesis method of the invention, and can also be used alone for detecting the genotoxic impurity (2S)-2-amino-3-ethoxysulfinyl-3-methylbutyric acid in the sulbactam sodium raw material, thereby ensuring the safety of the drug. The reaction raw materials of the synthesis method of the impurity are simple and easy to obtain, and the reaction is carried out in only one step, and the operation is simple.

This invention discloses a cefoperazone sodium and sulbactam sodium composition and its preparation method, comprising the following steps: preparing a chitosan-β-glycerophosphate sodium colloidal solution; mixing dry ice and sorbitol evenly, then adding the mixture to the colloidal solution, freezing, heating, and drying under reduced pressure to obtain chitosan-β-glycerophosphate sodium nanospheres; slowly adding cefoperazone sodium, sulbactam sodium, and chitosan-β-glycerophosphate sodium nanospheres to water for injection, stirring to dissolve; adjusting the pH to 7.1-7.5, adding an adsorbent for adsorption and filtration, adding dry ice; freeze-drying to obtain a cefoperazone sodium and sulbactam sodium mixed powder. The nanospheres of this invention, with a certain size gradient, are beneficial for the uniformity of the mixed powder of cefoperazone sodium and sulbactam sodium, resulting in a longer-lasting efficacy, better stability, lower hygroscopicity, and also playing an antibacterial role.

The invention discloses a sulbactam sodium crystal preparation method, and belongs to the technical field of medicines, sodium lactate is adopted as a salt forming agent for preparing sulbactam sodium, and the prepared sulbactam sodium product is high in stability, milder in reaction condition and suitable for industrial mass production.

The invention discloses a preparation method and application of cefoperazone sodium and sulbactam sodium for injection, and relates to the field of preparation methods of cefoperazone sodium and sulbactam sodium, and the preparation method comprises preparation of cefoperazone sodium and preparation of a mixed solution. The preparation of the mixed solution comprises the following steps: weighing sulbactam sodium and the prepared cefoperazone sodium, adding the sulbactam sodium and the prepared cefoperazone sodium into a sodium chloride injection, dissolving, adding a phosphate buffer solution, filtering, adding valine into the solution, dispersing, and filtering to obtain the mixed solution. Sequentially carrying out freeze-drying treatment, drying, crushing, screening and re-drying on the dispersed mixed solution to obtain the cefoperazone sodium sulbactam sodium for injection. The mass ratio of the cefoperazone sodium to the sulbactam sodium is (1-5): 1. The valine is added into the mixed solution and the process is optimized, so that the valine and the cefoperazone sodium can form hydrogen bonds and electrostatic interaction, and the purpose of improving the stability of the cefoperazone sodium sulbactam sodium for injection with high cefoperazone sodium content is achieved.

The utility model relates to a sulbactam sodium mother liquor pH value adjusting device, which comprises a reaction tank, an adjusting tank, a stirring mechanism and a pH value detector, when the pH value is adjusted, a part of sulbactam sodium mother liquor enters the reaction tank through a feed port of the sulbactam sodium mother liquor; the other part of sulbactam sodium mother liquor is conveyed to a vertical liquid conveying pipeline through a first bypass pipeline under the action of a first lifting pump, meanwhile, alkali liquor for adjustment is conveyed into the vertical liquid conveying pipeline through a discharging opening of an adjusting tank, and the alkali liquor and the sulbactam sodium mother liquor are mixed and then enter a reaction tank through the lower end of the vertical liquid conveying pipeline and an adjusting opening; the alkali liquor is mixed with more sulbactam sodium mother liquor after entering the reaction tank, on one hand, the stirring paddle assembly is used for stirring, and on the other hand, the mixed liquid is led out from the bottom of the reaction tank through the second lifting pump and then flows back into the reaction tank through the second bypass pipeline and the annular liquid separation pipe. According to the utility model, the adjusting time is shortened, and the pH value adjusting efficiency is obviously improved.

The invention discloses a culture medium and a method for culturing digestive tract tumors by using a three-dimensional tumor chip technology. The culture medium is prepared from the following components: 5-10% v / v of FBS, 20-30% v / v of a Ham's F12 nutrient solution, 0.10-0.15 ng / mL of a recombinant human epidermal growth factor (EGF), 20-30 ng / mL of hydrocortisone, 0.05-0.15 nM of cholera toxin, 4-6 g / mL of insulin, 9-11 M of Y-27632 dihydrochloride, 10-30 [mu] g / mL of cefoperazone sodium, 5-15 [mu] g / mL of sulbactam sodium and the balance of DMEM (Dulbecco Modified Eagle Medium). The culture medium not only reduces the pollution rate, but also is high in cell activity and has an immune microenvironment similar to that of an original tissue, so that it is possible that an in-vitro 3D-TSCs culture method evaluates the drug sensitivity of immunotherapy drugs for clinical digestive tract tumors, and the culture medium has very high clinical application value and market potential.

The invention discloses a method for detecting polymers in cefoperazone sodium and sulbactam sodium, and belongs to the technical field of medicine quality control. The invention discloses a method for detecting polymers in cefoperazone sodium and sulbactam sodium. Respectively injecting the test solution and the reference solution into a liquid chromatograph, and detecting the content of the polymer by adopting a high performance liquid chromatography; the chromatographic column is a reversed-phase liquid chromatographic column; the detection wavelength is 240 to 250 nm; the flow velocity of the mobile phase is 0.4-0.8 ml / min; the column temperature is 20-30 DEG C; according to the method, the content of the polymer can be directly tested in one step under the chromatographic condition through liquid chromatography detection, and compared with the prior art in which impurities and main components are separated through a one-dimensional detection method and then the impurities are subjected to two-dimensional chromatographic analysis, the method is simple and high in operability and has remarkable advantages; the method can meet the requirements of qualitative and quantitative analysis, and is good in reproducibility, high in specificity, strong in durability, high in detection accuracy and good in system adaptability.

The utility model relates to the technical field of filling and feeding devices, in particular to a filling and feeding device suitable for cefoperazone sodium and sulbactam sodium for injection. Comprising a bottom plate, a baffle is arranged above the periphery of the bottom plate, and a runner pipe is arranged below the right side of the bottom plate; through the structure of an abutting plate, a fixing column and a sealing ring, a circulation pipe is blocked, under the elastic action of a spring, the circulation pipe is always kept in a closed state without external force under the use mode that the abutting plate is matched with a lifting frame, after a filling bottle is upwards connected with the circulation pipe, the fixing column slides out of the circulation pipe, and liquid medicine can flow out of the filling bottle; through the sliding structure of the sliding frame on the connecting frame, different filling bottles can be adjusted to move and be placed below the runner pipe, through the multi-station switching design of the sliding frame, continuous filling is achieved, manual intervention is reduced, and through clamping connection of a clamping groove and an elastic piece, the situation that in the filling process, the sliding frame slides, liquid medicine overflows, and waste is caused is avoided.

The utility model discloses a filling device for cefoperazone sodium and sulbactam sodium, and relates to the technical field of medicine filling equipment. A filling device for cefoperazone sodium and sulbactam sodium comprises a rotating disc set used for bearing and transporting medicine bottles; the supporting frame is arranged on the side edge of the rotating disc set; the lifting assembly is mounted on the supporting frame; the filling assembly is connected with the bottom of the lifting assembly and used for filling the medicine bottles with cefoperazone sodium and sulbactam sodium; the anti-overflow assembly is fixed to the filling assembly and arranged around the medicine bottle by a circle. The problems that mixed powder flies and is wasted, and the filling weight is insufficient are solved.

The present invention discloses an ampicillin sodium and sulbactam sodium pharmaceutical composition for injection and a preparation method thereof. The preparation method includes Step 1: uniformly mixing ampicillin sodium and sulbactam sodium; Step 2: uniformly mixing water-soluble chitosan microspheres with the mixture obtained in Step 1; Step 3: uniformly mixing sodium carboxymethyl cellulose with the mixture obtained in Step 2. In the present invention, by mixing water-soluble chitosan microspheres with ampicillin sodium and sulbactam sodium, the water-soluble chitosan microspheres can be uniformly dispersed in ampicillin sodium and sulbactam sodium. Then, the added sodium carboxymethyl cellulose forms a hydrogen bond with the water-soluble chitosan microspheres, so that a film is formed on the outside of the water-soluble chitosan microspheres by sodium carboxymethyl cellulose, and ampicillin sodium and sulbactam sodium can be dispersed in the dispersion system formed by sodium carboxymethyl cellulose and water-soluble chitosan microspheres, thereby providing long-term and stable protection for ampicillin sodium and sulbactam sodium.

The invention discloses a sulbactam sodium crystallization process, a sulbactam sodium product prepared by the crystallization process is good in fluidity, low in impurity content and good in stability, the product quality can be improved, the medication safety can be guaranteed, the preparation process is simple and rapid, the production cost can be saved, and the sulbactam sodium crystallization process is suitable for industrial production.

The invention discloses a cefoperazone sodium and sulbactam sodium polymer for injection and a specific impurity analysis method, relates to the field of medicine detection, and aims to provide the cefoperazone sodium and sulbactam sodium polymer for injection, which is high in sensitivity and good in stability, and the specific impurity analysis method. Chromatographic conditions are as follows: a chromatographic column adopts a C18 column; the mobile phase A is a 1mmol / L-10 mmol / L tetrabutylammonium hydroxide solution, the pH value of the tetrabutylammonium hydroxide solution is adjusted to 3.0-4.0 by using a phosphoric acid solution, the mobile phase B is acetonitrile, and gradient elution is carried out; the column temperature is 25-40 DEG C; the flow velocity is 0.5 mL / min to 1.5 mL / min; the sample size is 10 [mu] L; the detection wavelength is 220nm; the sample concentration is 1 mg / mL to 10 mg / mL. The method has the advantages of high detection sensitivity, good reproducibility, high stability and the like, and is suitable for detecting cefoperazone sodium and sulbactam sodium polymers for injection and specific impurities.