439results about How to "Addressing drug resistance" patented technology

The invention belongs to the biotechnology field, and in particular relates to escherichia coli TolC antigen and antibody. The antibody has the specificity aiming to a sequence shown by SEQ ID NO:1. The invention also provides a gene sequence for encoding the escherichia coli TolC antigen and application of the antibody to preparing a drug for inhibiting the bacterial resistance. The anti-TolC fragment SNGYRDANGI antibody enhances the sensitivity of bacteria to antibiotic and can specifically and efficiently inhibit the functions of drug resistance outer membrane protein of escherichia coli, thereby solving the problem of the bacterial resistance. Compared with the traditional antibody, the antibody has higher safety and operability on the application of the bacterial resistance drug.

The invention discloses a plant disinfectant and a preparation method thereof. The plant disinfectant comprises the following raw materials: weeping forsythia, honeysuckle, liquorice root, golden thread, cortex phellodendri, raw rhubarb, safflower, flos caryophyllata, red paeony root and fineleaf schizonepeta herb. The preparation method comprises the following steps of pulverizing the raw materials, immersing the pulverized raw materials into distilled spirit, performing percolation, standing and filtering and adding peppermint oil and/or rose essential oil to obtain products of the plant disinfectant through subpackage. The plant disinfectant combines various plant raw materials with disinfection and sterilization, contains compound chemical components, can kill various bacteria and virus, solves the problems of the drug resistance, drug tolerance, dependence, accumulated toxicity and the like of the bacteria and the virus fundamentally, has no pollution to environment, and is an efficient disinfectant which has broad spectrum of sterilization and disinfection, safety, no toxic or side effect, quick response and stable properties.

The invention discloses a preparation method for a crosslinked gelatin antimicrobial film material. The method comprises the following steps of: (1) adding glycerol into gelatin aqueous solution; (2) adding an antimicrobial agent into the solution obtained in the step (1) and dissolving with stirring; (3) adding a crosslinking agent and reacting at the temperature of 45 to 55 DEG C for 20 to 40 minutes under the condition of magnetic stirring; and (4) performing vacuum degassing on the material obtained in the step (3) to obtain the film, wherein the antimicrobial agent is nisin or lysozyme. By the method, the release speed of the antimicrobial agent in the film material can be scientifically adjusted and the purpose of controlled release is realized. The crosslinked gelatin antimicrobial film material can be applied to an inner packaging material for food and aims to solve the food safety problem caused by that the microorganisms grow on the surface in the storage process of the food. The protein-lysozyme/nisin composite antimicrobial film material prepared by the method has great application potency of improving food quality and prolonging the shelf life of the food.

The invention discloses a novel dual inhibitor of deoxyribonucleic acid (DNA) topoisomerase I and DNA topoisomerase II. Mononuclear ruthenium (II) complexes containing a plurality of hydroxy ligands are synthesized, the complexes are stable in structure, the water-solubility of the complexes is better than that of normal organic small molecule inhibitors, and the complexes have dual poison effect for I type DNA topoisomerase and II type DNA topoisomerase and can inhibit body cancer cell growth obviously.

The invention discloses a preparation method of a self-assembled lysozyme multilayer membrane with an antibacterial property and biocompatibility, and belongs to the technical field of preparation of biomedical materials. The preparation method comprises the following steps: preparing a self-assembled multilayer membrane substrate, preparing a plant polyphenol solution, preparing a lysozyme solution, preparing the self-assembled multilayer membrane and the like. With a strong hydrogen-bond interaction between molecules of plant polyphenol and lysozyme as a driving force in the deposition process, the main part of the self-assembled membrane does not use polycations, so that cell damage caused by multiple layers of polycations in the conventional self-assembled membrane can be effectively reduced; after the surface of a human implant is decorated with the self-assembled multilayer membrane, the antibacterial property and the biocompatibility of the implant can be significantly improved; and the self-assembled multilayer membrane is very wide in application prospect.

The invention relates to an anti-mosquito incense containing plant vinegar and application thereof, belonging to the sanitary insecticide article technical field. When made into a solid sheet type, the anti-mosquito incense comprises 45 to 65 percent of plant vinegar, 35 to 55 percent of binder and 0.1 to 1.0 percent of essence; when made into a liquid type, the anti-mosquito incense comprises 90 to 99 percent of plant vinegar, and 4 to 8 percent of essence. The plant vinegar is one or a plurality of bamboo vinegar, wood vinegar and/or grass vinegar; the binder is carbon powder, flour, corn starch, polyvinyl alcohol, WX-602 gelatine powder produced by Juxiang (a name of a company) and sticky wood powder; the essence is one or a plurality of benzyl acetate, citric acid, wintergreen oil, dodecanoic acid, Australian orange essence oil, 2, 3-diacetyl propyl lauric acid ester, menthol and citronella oil. The anti-mosquito incense adopts plant vinegar as the active ingredient, has three functions of killing mosquito, killing fly and killing cockroach with good control effect and solves the problem that the using of chemical anti-mosquito incense can cause the insect to generate resistance to drugs.

The invention relates to a traditional Chinese medicine microecological preparation for improving the immunity of chicks, and a preparation method and usage of the traditional Chinese medicine microecological preparation, belonging to the technical field of veterinary traditional Chinese medicine microecological preparations. The traditional Chinese medicine microecological preparation is prepared by carrying out mixed fermentation on fermentation strains and the following raw materials: 15-20 parts of radix astragali seu hedysari, 5-10 parts of Chinese angelica, 5-10 parts of fructus ligustri lucidi, 5-10 parts of radix isatidis, 5-10 parts of folium isatidis, 15-20 parts of cortex eucommiae, 5-10 parts of fructus crataegi, 5-10 parts of malt, 1-2 parts of brown sugar, 1-3 parts of fructo-oligosaccharide and 1-3 parts of lysine; the fermentation strains comprise a bacillus subtilis strain, a lactobacillus plantarum strain, an enterococcus faecium strain and saccharomyces cerevisiae. The traditional Chinese medicine microecological preparation can enhance the immunity of the chicks and improve the digestion-absorption function of the intestinal tracts of the chicks, thus achieving the aims of increasing the disease resistance of the chicks as well as reducing the morbidity and mortality of the chicks on the whole, and increasing the economic benefit.

The invention relates to a composition of ceftriaxone sodium and tazobactam sodium, which comprises active constituents of ceftriaxone sodium and tazobactam sodium by the weight ratio of 1-10:1, preferably 2-8:1, optimally 4:1. The compound preparation can appreciably reinforce the antibiotic action to Gram-negative bacteria.

The invention relates to a compound preparation of ceftiofur sodium, in particular to the application of a compound preparation of ceftiofur sodium in preparing medicines for treating colibacillus or salmonella infection of chicken and swine. The compound preparation of ceftiofur sodium comprises the following formula in parts by weight: 0.5-8 parts of ceftiofur sodium, 0.5-6 parts of fosfomycin sodium, 1-3 parts of sulbactam sodium and 4-7 parts of enorfloxacin sodium. The formula can be prepared into a premixed agent, a drink agent or an injection in the using process. The applicant of the invention, by summarizing of many years of tests and according to the antibacterial synergy principle, prepares the compound preparation of ceftiofur sodium which effectively solves the drug tolerance problem of the colibacillus and salmonella, simultaneously improves the action effect and the field of application and improves the survival rate of livestock and fowl.

The present invention relates to a method for making biological inhibition of sea water cooling system. It is characterized by that in the cooling system adopting sea water or fresh water of estuary as cooling water the non-oxidative biocide and oxidative biocide can be alternatively added. Besides, said invention also provides their concrete alternative addition mode.

The invention discloses a biopesticide for vegetables and a preparation method of the biopesticide. The biopesticide is mainly prepared from the components in parts by weight as follows: 1 part of toyocamycin, 50-70 parts of common threewingnut roots, 60-80 parts of graceful jessamine herb, 40-60 parts of aconite, 50-70 parts of coptis chinensis, 40-60 parts of purslane, 30-50 parts of herba houttuyniae, 40-60 parts of common andrographis herb and 2-4 parts of a suspending agent. Compared with common chemical pesticides in the prior art, the obtained biopesticide for the vegetables has the characteristics of good effect and low toxicity and can be used as an ideal environment-friendly agricultural drug for vegetable protection; meanwhile, the toyocamycin is compounded with traditional Chinese medicine extracts, so that the sterilization and insecticidal spectrum can be enlarged, the efficacy can also be improved, and what's more, the problem of drug resistance caused by long-term usage of single pesticides can be solved.

The present invention provides an application of a protein in preventing and treating spodoptera frugiperda and/or spodoptera litura. The amino acid sequence of the protein is shown in SEQ ID No.4.

The invention relates to a bactericidal composition comprising the active components including iprovalicarb (A) and B which is one of metalaxyl, benalaxyl, furalaxyl, oxadixyl, propamocarb hydrochloride and metalaxyl-M. The mass ratio of A to B is 90:1-1:90. The composition of the invention is especially suitable for controlling the oomycete diseases of cereals, vegetables, rape, fruits, cotton and ornamental plants, including downy mildew, plagues and late blight, etc.

The invention discloses preparation of bacterium targeting nanoparticles and application of the bacterium targeting nanoparticles to bacterium inhibition and killing. The bacterium targeting nanoparticles are prepared by carrying out self-assembling on an amphiphilic polymer with a structure shown as a formula (I) and a targeting segment. The preparation comprises the following steps: preparing apoly(N,N-dimethylaminoethyl methacrylate)-copolymerized-polymerized photosensitizer by utilizing a photosensitizer and dimethylaminoethyl methacrylate; then taking the poly(N,N-dimethylaminoethyl methacrylate)-copolymerized-polymerized photosensitizer as a macromolecular chain transferring agent and taking n-butyl methacrylate as a hydrophobic unit, and carrying out polymerization of a second block; activating carboxyl and connecting with a bacterium targeting peptide; carrying out the self-assembling to form the nanoparticles capable of being dissolved in water. The obtained nanoparticles canbe selectively combined with bacteria through the bacterium targeting peptide, so that the safety of a material is improved; the antibacterial activity of the material is improved through a photodynamic and physical effect combined antibacterial manner; meanwhile, the bacteria do not easily have drug resistance, and the bacterium targeting nanoparticles can be widely applied to the fields of bacterium inhibition and sterilization. The formula (I) is shown in the description.

The invention discloses an antifolic for anti-tumor therapy, a relative salt and intermediate, wherein the antifolic and relative salt are represented as below, R1=NH2, R2=CH3, or R1=NHCH3 and R2=H, Ar is 1, 4-phenyl or 2, 5-thiofuran, R3 is H, metal cation, ammonium ion, or organic ammonium cation. The invention further discloses an application of the antifolic and relative salt for preparing anti-cancer drug. The inventive antifolic and relative salt have significant growth restraining activity on multiple tumor cells.

The invention discloses a double-target anti-HIV (anti-human immunodeficiency virus) glycopeptide compound and an application thereof. The anti-HIV glycopeptide compound is a T20 compound modified with mannan oligosaccharide or oligosaccharide containing fucose at the terminal. The fixed-point glycosylated modified compound of an anti-HIV drug represented as M1-T20, M3-T20, M5-T20 or Lex-T20 is a double-target drug, can prolong the plasma half-life of the drug in the animals and enhance the HIV inhibiting effect, and has the great application value.

The invention discloses a method for screening influenza virus resisting medicines. In the method, a sample to be detected is added into a fusion protein cell which expresses influenza virus ribonucleic acid (RNA) polymerase and an activity-dependent reporter gene thereof, and the quantity of a coding product of the reporter gene is observed, so that the blocking effect of the sample on the RNA polymerase is determined; and whether the sample subjected to primary selection is an RNA polymerase activity inhibitor or not is further judged, so that the influenza virus resisting medicines are screened. By the screening method, an influenza virus RNA polymerase complex is taken as a target, so that the problem of medicine resistance caused by high virus variation is solved, and the cross medicine resistance of the screened influenza virus resisting medicines and the conventional clinically applied influenza virus resisting medicines is avoided.

The present invention provides application of Merremia boisiana extract in preparing bactericide. Merremia boisiana extract has broad-spectrum and high bacteriostasis activity on plant pathogenic fungi, food mildew and plant pathogenic bacteria, and high preventing and treating effect on sheath and cullm blight of rice and frosty mildew of cucumber. The extract is safe to crops and easy to biodegrade, and has no residue and no pollution. In addition, it has action mechanism on plant pathogenic fungi, food mildew and plant pathogenic bacteria dissimilar to common bactericides and thus less resistance. The present invention provides one new kind of bactericide while paving one way of controlling Merremia boisiana as one harmful forest plant.

The invention provides a biological insecticide containing lycopene and a preparation method of the biological insecticide. The biological insecticide is mainly prepared from components in parts by weight as follows: 0.5 parts of avilamycin, 5-15 parts of tomato leaves, 5-15 parts of snakegourd roots, 10-20 parts of radix astragali, 5-25 parts of pyrethrum, 10-25 parts of radix rehmanniae, 60-100 parts of soybean oil and 0.1-0.5 part of an emulsifier. Compared with an ordinary chemical pesticide in the prior art, the biological insecticide containing lycopene has the characteristics of good effect and low toxicity and can be used as an ideal environment-friendly agricultural plant protection drug; meanwhile, two or more pesticides are compounded, so that on one hand, the bactericidal and insecticidal spectrum can be widened, and on the other hand, the pesticide effect can be improved; and more importantly, the problem of drug resistance due to the fact that a single pesticide is used for a long term can be solved.

The invention discloses a compound biological pesticide and a preparation method thereof. The compound biological pesticide is prepared by components in parts by weight as follows: 0.1 part of lentinan, 1-5 parts of amino acid, 10-20 parts of bupleurum, 10-30 parts of codonopsis pilosula, 10-20 parts of aconitine, 15-20 parts of radix euphorbiae lantu, 15-25 parts of ephedra and 1-5 parts of a suspending aid. Compared with ordinary chemical pesticide in the prior art, the prepared compound biological pesticide has the characteristics of good effect and low toxicity and can be used as an ideal environment-friendly agricultural plant protection drug; meanwhile, two or more pesticides are compounded, so that on one hand, the bactericidal and insecticidal spectrum can be widened, and on the other hand, the pesticide effect can be improved; and more importantly, the problem of drug resistance due to the fact that a single pesticide is used for a long term is solved.

The medicine composition has synergistic active components trifusal and clopidogrel, and its clopidogrel exists in free state or pharmaceutically acceptable salt. The medicine composition is used in treating stable or unstable angina pectoris and other cardiac and cerebral vascular diseases. Pharmaceutical experiments prove the synergistic effect of trifusal and clopidogrel in the medicine composition and the medicine composition has blood platelet inhibiting rate superior to that of aspirin-clopidogrel composition and high safety suitable for long -term taking.