440results about How to "Addressing drug resistance" patented technology

The invention belongs to the biotechnology field, and in particular relates to escherichia coli TolC antigen and antibody. The antibody has the specificity aiming to a sequence shown by SEQ ID NO:1. The invention also provides a gene sequence for encoding the escherichia coli TolC antigen and application of the antibody to preparing a drug for inhibiting the bacterial resistance. The anti-TolC fragment SNGYRDANGI antibody enhances the sensitivity of bacteria to antibiotic and can specifically and efficiently inhibit the functions of drug resistance outer membrane protein of escherichia coli, thereby solving the problem of the bacterial resistance. Compared with the traditional antibody, the antibody has higher safety and operability on the application of the bacterial resistance drug.

The invention provides a preparation method of anti-infection coating. The preparation method comprises the following steps of (a) performing photoinitiator painting treatment on the surfaces of a medical macromolecule material to obtain a base material; (b) placing the base material obtained in the step (a) in a mixed solution comprising adhesion-resistant monomers, pH responsiveness polymerizingmonomers and polyethylene glycol diacrylate, and performing an ultraviolet cross-linking reaction to obtain a material having gel coating on the surface; and (c) performing antibiotic chemical loading on the material having gel coating on the surface obtained in the step (b) so as to obtain the anti-infection coating. According to the preparation method provided by the invention, a specific technology and a specific condition are adopted, and the anti-infection coating is obtained. The anti-infection coating has dual functions of resisting bacterium adhesion and controlling antibiotics to release for sterilization, so that bacterium adhesion can be effectively reduced, controllable release of antibiotics as required can be realized, and the problems that the bacterium-resistant effect ispoor in persistence caused by too fast antibiotic release and bacteria produce drug tolerance caused by random release of the antibiotics can be solved.

The invention relates to the fields of microorganism preparations and aquaculture, and discloses a lysozyme microcapsule. The microbial lysozyme microcapsule is prepared by the following steps of: (1) dropping a mixing solution of sodium alginate and lysozyme into an acetic acid solution containing chitosan and calcium chloride, and stirring; (2) after dropping, curing for 0.5-4hours, and taking and washing a solid to obtain lysozyme / sodium alginate / chitosan beads; and (3) pre-freezing the lysozyme / sodium alginate / chitosan beads, and freeze-drying. The microcapsule disclosed by the invention is used for preparing fish feed, and is capable of reserving comparatively high activity of the microbial lysozyme after being extruded at the high temperature in the feed processing; in the process of conserving at normal temperature, the stability of the microbial lysozyme can be kept for more than half an year; and in feeding and breeding, the microbial lysozyme can bear the strong acid and digesting environment in the stomach of the aquatic animal, release in the intestinal tract and develop an effective growth-promoting healthcare effect.

The invention discloses a plant disinfectant and a preparation method thereof. The plant disinfectant comprises the following raw materials: weeping forsythia, honeysuckle, liquorice root, golden thread, cortex phellodendri, raw rhubarb, safflower, flos caryophyllata, red paeony root and fineleaf schizonepeta herb. The preparation method comprises the following steps of pulverizing the raw materials, immersing the pulverized raw materials into distilled spirit, performing percolation, standing and filtering and adding peppermint oil and / or rose essential oil to obtain products of the plant disinfectant through subpackage. The plant disinfectant combines various plant raw materials with disinfection and sterilization, contains compound chemical components, can kill various bacteria and virus, solves the problems of the drug resistance, drug tolerance, dependence, accumulated toxicity and the like of the bacteria and the virus fundamentally, has no pollution to environment, and is an efficient disinfectant which has broad spectrum of sterilization and disinfection, safety, no toxic or side effect, quick response and stable properties.

The invention belongs to the field of nucleoside drugs, more particularly relates to a prodrug based on a gemcitabine structure shown as a formula (I) as well as a synthesizing method and the application thereof, wherein a is equal to an integer from 0 to 6; b is equal to an integer from 0 to 6; c is equal to an integer from 0 1 to 8; d is equal to an integer from 1 to 4; E is five-membered or six-membered ring alkyl, five-membered or six-membered ring naphthene base containing 1-4 hetero atoms, aryl or heteroaryl. The hetero atoms are selected from O, N or S; Z is selected from O, N or S; and V is selected from hydrogen, alkyl, alkoxyl, ester group, halogen, amido, amino or substituted amino. The invention utilizes N<4> modification to increase the solubility, the bioavailability and the organ specificity of the prodrug, and the generated prodrug compound can solve the problem of fast metabolization, thereby improving the capacities of antitumor, anticancer, anti-infection and diffusion prevention and being capable of specifically acting on liver or colon. The synthesizing method has simple procedures, easily-obtained materials, high productivity rate and low cost and is suitable for industrial production.

The invention belongs to the field of hygiene and relates to a bacillus subtillus degrading bacterial colony sensing signal and use of bacillus subtillus degrading bacterial colony as an antiseptic, in particular to bacillus subtillus degrading gram-negative bacterial colony sensing signal and the formation of a bacterial biofilm of the bacillus subtillus degrading gram-negative bacterial colony. In the use of the degrading bacterial colony sensing signal, a proper concentration of the bacillus subtillus is 5*102 to 5*104cfu / ml. The bacillus subtillus is further used as antiseptic to inhibit the bacteria such as pseudomonas, agrobacterium, enterobacteriaceae and vibrionaceae. The antiseptic can be ointment for external use, liquid preparation for external use, oral tablet, oral liquid, disinfectant, clearing agents or water quality improving agent. The consistency of the bacillus subtillus serving as the antiseptic is 105 to 106 cfu / g. The use of the bacillus subtillus provided by the invention and the antiseptic inhibit the formation of biofilm of disease-causing bacteria and the growth of bacteria by using microbial quorum-sensing inhibition instead of preventing and sterilizing bacteria by compound which is adopted by most of the conventional antiseptic; therefore, the problem of drug resistance in the antimicrobial process is avoided.

The invention discloses a preparation method for a crosslinked gelatin antimicrobial film material. The method comprises the following steps of: (1) adding glycerol into gelatin aqueous solution; (2) adding an antimicrobial agent into the solution obtained in the step (1) and dissolving with stirring; (3) adding a crosslinking agent and reacting at the temperature of 45 to 55 DEG C for 20 to 40 minutes under the condition of magnetic stirring; and (4) performing vacuum degassing on the material obtained in the step (3) to obtain the film, wherein the antimicrobial agent is nisin or lysozyme. By the method, the release speed of the antimicrobial agent in the film material can be scientifically adjusted and the purpose of controlled release is realized. The crosslinked gelatin antimicrobial film material can be applied to an inner packaging material for food and aims to solve the food safety problem caused by that the microorganisms grow on the surface in the storage process of the food. The protein-lysozyme / nisin composite antimicrobial film material prepared by the method has great application potency of improving food quality and prolonging the shelf life of the food.

The invention discloses a novel dual inhibitor of deoxyribonucleic acid (DNA) topoisomerase I and DNA topoisomerase II. Mononuclear ruthenium (II) complexes containing a plurality of hydroxy ligands are synthesized, the complexes are stable in structure, the water-solubility of the complexes is better than that of normal organic small molecule inhibitors, and the complexes have dual poison effect for I type DNA topoisomerase and II type DNA topoisomerase and can inhibit body cancer cell growth obviously.

The invention discloses a preparation method of a self-assembled lysozyme multilayer membrane with an antibacterial property and biocompatibility, and belongs to the technical field of preparation of biomedical materials. The preparation method comprises the following steps: preparing a self-assembled multilayer membrane substrate, preparing a plant polyphenol solution, preparing a lysozyme solution, preparing the self-assembled multilayer membrane and the like. With a strong hydrogen-bond interaction between molecules of plant polyphenol and lysozyme as a driving force in the deposition process, the main part of the self-assembled membrane does not use polycations, so that cell damage caused by multiple layers of polycations in the conventional self-assembled membrane can be effectively reduced; after the surface of a human implant is decorated with the self-assembled multilayer membrane, the antibacterial property and the biocompatibility of the implant can be significantly improved; and the self-assembled multilayer membrane is very wide in application prospect.

The invention discloses a compounded insecticidal suspension emulsion with chlorpyrifos and spirotetramat and a preparation method of the compounded insecticidal suspension emulsion. The compounded insecticidal suspension emulsion comprises the following contents in percentage by weight: 2-30% of spirotetramat, 10-40% of chlorpyrifos, 5-20% of organic solvent and the balance water, and is formed by adopting the following steps of mechanical stirring, sanding and high-speed homogeneity cutting. The compounded insecticidal suspension emulsion has a stable property and a high medicine effect, and can be used for preventing injurious insects such as scale insects of oranges, cotton and apples, bemisia tabaci of vegetables, and the like. According to the invention, the compounded insecticidal suspension emulsion has the advantages that the water is used as the main dispersion medium, the pollution to the environment is small, the situations of oil deposition, sediment and the like when the compounded insecticidal suspension emulsion is stored for two years under normal temperature are not existed, the service life of a storage rack is long and is non-flammable, the storage and the transportation is safe, compared with using the spirotetramat alone, the cost is low, and the like.

The invention relates to an anti-mosquito incense containing plant vinegar and application thereof, belonging to the sanitary insecticide article technical field. When made into a solid sheet type, the anti-mosquito incense comprises 45 to 65 percent of plant vinegar, 35 to 55 percent of binder and 0.1 to 1.0 percent of essence; when made into a liquid type, the anti-mosquito incense comprises 90 to 99 percent of plant vinegar, and 4 to 8 percent of essence. The plant vinegar is one or a plurality of bamboo vinegar, wood vinegar and / or grass vinegar; the binder is carbon powder, flour, corn starch, polyvinyl alcohol, WX-602 gelatine powder produced by Juxiang (a name of a company) and sticky wood powder; the essence is one or a plurality of benzyl acetate, citric acid, wintergreen oil, dodecanoic acid, Australian orange essence oil, 2, 3-diacetyl propyl lauric acid ester, menthol and citronella oil. The anti-mosquito incense adopts plant vinegar as the active ingredient, has three functions of killing mosquito, killing fly and killing cockroach with good control effect and solves the problem that the using of chemical anti-mosquito incense can cause the insect to generate resistance to drugs.

The invention relates to a traditional Chinese medicine microecological preparation for improving the immunity of chicks, and a preparation method and usage of the traditional Chinese medicine microecological preparation, belonging to the technical field of veterinary traditional Chinese medicine microecological preparations. The traditional Chinese medicine microecological preparation is prepared by carrying out mixed fermentation on fermentation strains and the following raw materials: 15-20 parts of radix astragali seu hedysari, 5-10 parts of Chinese angelica, 5-10 parts of fructus ligustri lucidi, 5-10 parts of radix isatidis, 5-10 parts of folium isatidis, 15-20 parts of cortex eucommiae, 5-10 parts of fructus crataegi, 5-10 parts of malt, 1-2 parts of brown sugar, 1-3 parts of fructo-oligosaccharide and 1-3 parts of lysine; the fermentation strains comprise a bacillus subtilis strain, a lactobacillus plantarum strain, an enterococcus faecium strain and saccharomyces cerevisiae. The traditional Chinese medicine microecological preparation can enhance the immunity of the chicks and improve the digestion-absorption function of the intestinal tracts of the chicks, thus achieving the aims of increasing the disease resistance of the chicks as well as reducing the morbidity and mortality of the chicks on the whole, and increasing the economic benefit.

The invention relates to a low concentration guanidine gum fracturing fluid for gas well. The fracturing fluid is composed of hydroxypropyl guar gum, an anti-swelling stabilizer, a bactericide, a foaming cleanup additive, a conditioning agent, a temperature stabilizing agent, a chelating agent, a crosslinking agent, a gel breaker, and water. The provided fracturing fluid overcomes the shortages of a conventional fracturing fluid for gas well such as a large using amount of thickening agent, a large amount of residues after gel breaking, and the like. The preparation cost is low. The performance on resisting temperature and shearing is good. The using concentration of guanidine gum is greatly reduced. The damage on stratum is obviously reduced. Prominent economic benefit is created, and the application prospect is wide.

The invention relates to a composition of ceftriaxone sodium and tazobactam sodium, which comprises active constituents of ceftriaxone sodium and tazobactam sodium by the weight ratio of 1-10:1, preferably 2-8:1, optimally 4:1. The compound preparation can appreciably reinforce the antibiotic action to Gram-negative bacteria.

The invention relates to a compound preparation of ceftiofur sodium, in particular to the application of a compound preparation of ceftiofur sodium in preparing medicines for treating colibacillus or salmonella infection of chicken and swine. The compound preparation of ceftiofur sodium comprises the following formula in parts by weight: 0.5-8 parts of ceftiofur sodium, 0.5-6 parts of fosfomycin sodium, 1-3 parts of sulbactam sodium and 4-7 parts of enorfloxacin sodium. The formula can be prepared into a premixed agent, a drink agent or an injection in the using process. The applicant of the invention, by summarizing of many years of tests and according to the antibacterial synergy principle, prepares the compound preparation of ceftiofur sodium which effectively solves the drug tolerance problem of the colibacillus and salmonella, simultaneously improves the action effect and the field of application and improves the survival rate of livestock and fowl.

The invention discloses a pymetrozine-resistant isaria javanica IJNL-N8 strain and application thereof. The strain is preserved in CCTCC (China Center For Type Culture Collection) on November 22, 2017, and the collection number is CCTCC NO:M 2017709. According to the invention, the isaria javanica IJNL-N8 strain with high pathogenicity to rice planthopper is obtained by screening, and by virtue oflong-term infection biology and indoor biological assay, results show that the strain has a very high insecticidal effect on rice planthopper. The isaria javanica IJNL-N8 strain is a biological control fungus, serves as a living biological insecticide, has bran-new action mechanisms different from existing chemical insecticides, does not pollute the environment, has no residue, can solve the problems of drug resistance and pesticide residue in prevention and treatment processes of rice planthopper, meets the requirements for production of organic food, and has excellent application potentialin biological prevention and treatment of rice pests.

The present invention relates to a method for making biological inhibition of sea water cooling system. It is characterized by that in the cooling system adopting sea water or fresh water of estuary as cooling water the non-oxidative biocide and oxidative biocide can be alternatively added. Besides, said invention also provides their concrete alternative addition mode.

The present invention discloses a polyether ether ketone bone repair material with antibacterial properties and a preparation method of the polyether ether ketone bone repair material. Cobalt nanowires and Ti<3>C<2> are compounded to delay combination of electrons and holes in Ti<3>C<2> energy bands and increase the transferred electrons to form singlet oxygen to solve a limiting problem of an antibacterial effect of the Ti<3>C<2>, thereby preventing SPEEK implants from infecting bacteria and inhibiting production of drug-resistant bacteria. In addition, polyether ether ketone is placed in concentrated sulfuric acid for sulfonation to obtain sulfonated polyether ether ketone with rough and porous surface and sulfonic acid groups, which increases contact areas of a Ti<3>C<2>-CoNWs compositematerial and SPEEK and improving a binding force between the Ti<3>C<2>-CoNWs composite material and the SPEEK; and sulfonic acid groups on the surface of the SPEEK react with hydroxyl groups on the surface of the Ti<3>C<2> to form sulfonate, so that a chemical bond connection also exists between the SPEEK and Ti<3>C<2>-CoNWs composite material, so as to further strengthen the bonding force between the Ti<3>C<2>-CoNWs composite material and the SPEEK and avoid a problem of Ti<3>C<2>-CoNWs composite material falling off from the SPEEK during use.

The invention discloses a biopesticide for vegetables and a preparation method of the biopesticide. The biopesticide is mainly prepared from the components in parts by weight as follows: 1 part of toyocamycin, 50-70 parts of common threewingnut roots, 60-80 parts of graceful jessamine herb, 40-60 parts of aconite, 50-70 parts of coptis chinensis, 40-60 parts of purslane, 30-50 parts of herba houttuyniae, 40-60 parts of common andrographis herb and 2-4 parts of a suspending agent. Compared with common chemical pesticides in the prior art, the obtained biopesticide for the vegetables has the characteristics of good effect and low toxicity and can be used as an ideal environment-friendly agricultural drug for vegetable protection; meanwhile, the toyocamycin is compounded with traditional Chinese medicine extracts, so that the sterilization and insecticidal spectrum can be enlarged, the efficacy can also be improved, and what's more, the problem of drug resistance caused by long-term usage of single pesticides can be solved.

The present invention provides an application of a protein in preventing and treating spodoptera frugiperda and / or spodoptera litura. The amino acid sequence of the protein is shown in SEQ ID No.4.

The invention relates to a bactericidal composition comprising the active components including iprovalicarb (A) and B which is one of metalaxyl, benalaxyl, furalaxyl, oxadixyl, propamocarb hydrochloride and metalaxyl-M. The mass ratio of A to B is 90:1-1:90. The composition of the invention is especially suitable for controlling the oomycete diseases of cereals, vegetables, rape, fruits, cotton and ornamental plants, including downy mildew, plagues and late blight, etc.

The invention discloses preparation of bacterium targeting nanoparticles and application of the bacterium targeting nanoparticles to bacterium inhibition and killing. The bacterium targeting nanoparticles are prepared by carrying out self-assembling on an amphiphilic polymer with a structure shown as a formula (I) and a targeting segment. The preparation comprises the following steps: preparing apoly(N,N-dimethylaminoethyl methacrylate)-copolymerized-polymerized photosensitizer by utilizing a photosensitizer and dimethylaminoethyl methacrylate; then taking the poly(N,N-dimethylaminoethyl methacrylate)-copolymerized-polymerized photosensitizer as a macromolecular chain transferring agent and taking n-butyl methacrylate as a hydrophobic unit, and carrying out polymerization of a second block; activating carboxyl and connecting with a bacterium targeting peptide; carrying out the self-assembling to form the nanoparticles capable of being dissolved in water. The obtained nanoparticles canbe selectively combined with bacteria through the bacterium targeting peptide, so that the safety of a material is improved; the antibacterial activity of the material is improved through a photodynamic and physical effect combined antibacterial manner; meanwhile, the bacteria do not easily have drug resistance, and the bacterium targeting nanoparticles can be widely applied to the fields of bacterium inhibition and sterilization. The formula (I) is shown in the description.

The invention discloses an antifolic for anti-tumor therapy, a relative salt and intermediate, wherein the antifolic and relative salt are represented as below, R1=NH2, R2=CH3, or R1=NHCH3 and R2=H, Ar is 1, 4-phenyl or 2, 5-thiofuran, R3 is H, metal cation, ammonium ion, or organic ammonium cation. The invention further discloses an application of the antifolic and relative salt for preparing anti-cancer drug. The inventive antifolic and relative salt have significant growth restraining activity on multiple tumor cells.

The invention discloses a double-target anti-HIV (anti-human immunodeficiency virus) glycopeptide compound and an application thereof. The anti-HIV glycopeptide compound is a T20 compound modified with mannan oligosaccharide or oligosaccharide containing fucose at the terminal. The fixed-point glycosylated modified compound of an anti-HIV drug represented as M1-T20, M3-T20, M5-T20 or Lex-T20 is a double-target drug, can prolong the plasma half-life of the drug in the animals and enhance the HIV inhibiting effect, and has the great application value.

The invention relates to a preparation technology of an improved oil field composite bactericide. The technical scheme is that the preparation technology comprises the following steps in parts by weight: (1) adding 15-20 parts of BC-2 multiple quaternary ammonium salt bactericide, 8-10 parts of polyhexamethylene guanidine , 8-10 parts of tetrakis hydroxymethyl phosphonium sulfate, 2-5 parts of methylisothiazolinone, 1-3 parts of medical metronidazole, 1-3 parts of dithiocyano-methane, 8-10 parts of glutaraldehyde, 1-2 parts of isopropanol, 1-2 parts of ethylene glycol and 0.1-0.5 part of nonylphenol polyoxyethylene ether-10 into a preparation kettle, then adding 30-40 parts of water, and sufficiently stirring; and (2) adding a sodium hydroxide aqueous solution, and adjusting the pH value to be between 7 and 9. The preparation technology has the beneficial effects that the bactericide also reaches the following requirements while solving the problem of bacterial resistance: (1) the bactericide is high in bactericidal ability and low in lethal concentration; (2) the bactericide is wide in application range and can be resistant to polymer-contained sewage; (3) the bactericide is good in water solubility, convenient to add and good in compatibility with other water treatment agents; and (4) the bactericide is low in cost.

The invention discloses a method for screening influenza virus resisting medicines. In the method, a sample to be detected is added into a fusion protein cell which expresses influenza virus ribonucleic acid (RNA) polymerase and an activity-dependent reporter gene thereof, and the quantity of a coding product of the reporter gene is observed, so that the blocking effect of the sample on the RNA polymerase is determined; and whether the sample subjected to primary selection is an RNA polymerase activity inhibitor or not is further judged, so that the influenza virus resisting medicines are screened. By the screening method, an influenza virus RNA polymerase complex is taken as a target, so that the problem of medicine resistance caused by high virus variation is solved, and the cross medicine resistance of the screened influenza virus resisting medicines and the conventional clinically applied influenza virus resisting medicines is avoided.

The present invention provides application of Merremia boisiana extract in preparing bactericide. Merremia boisiana extract has broad-spectrum and high bacteriostasis activity on plant pathogenic fungi, food mildew and plant pathogenic bacteria, and high preventing and treating effect on sheath and cullm blight of rice and frosty mildew of cucumber. The extract is safe to crops and easy to biodegrade, and has no residue and no pollution. In addition, it has action mechanism on plant pathogenic fungi, food mildew and plant pathogenic bacteria dissimilar to common bactericides and thus less resistance. The present invention provides one new kind of bactericide while paving one way of controlling Merremia boisiana as one harmful forest plant.

The invention provides a biological insecticide containing lycopene and a preparation method of the biological insecticide. The biological insecticide is mainly prepared from components in parts by weight as follows: 0.5 parts of avilamycin, 5-15 parts of tomato leaves, 5-15 parts of snakegourd roots, 10-20 parts of radix astragali, 5-25 parts of pyrethrum, 10-25 parts of radix rehmanniae, 60-100 parts of soybean oil and 0.1-0.5 part of an emulsifier. Compared with an ordinary chemical pesticide in the prior art, the biological insecticide containing lycopene has the characteristics of good effect and low toxicity and can be used as an ideal environment-friendly agricultural plant protection drug; meanwhile, two or more pesticides are compounded, so that on one hand, the bactericidal and insecticidal spectrum can be widened, and on the other hand, the pesticide effect can be improved; and more importantly, the problem of drug resistance due to the fact that a single pesticide is used for a long term can be solved.

The invention relates to a natural antibiotic peptide HKPLP derived from hippocampus and a gene hkplp for coding the protein. The DNA sequence of the natural antibiotic peptide HKPLP is represented by the sequence No.1 in a sequence table, and the protein coded by the gene (HKPLP) is represented by the sequence No.2 in the sequence table. In the invention, a new hkplp antibiotic peptide gene is designed according to the preferred codons of the pichia pastoris, the DNA sequence of the gene is synthesized and transferred to the pichia pastoris KM71 to be expressed, and thus, the pichia pastoris engineering strain of the antibiotic peptide is formed. Meanwhile, the fermentation conditions of the strain are optimized to realize high-density fermentation and high-efficiency expression. The fermentation liquor of the engineering strain has obvious inhibition effects on Gram positive bacteria, Gram negative bacterial, fungi and the like. The processed product of the fermentation liquor was added into feed for shrimp, chicken and the like, and good experimental effects were obtained. The antibiotic peptide product can replace antibiotic, prevent diseases of the digestive tract, promote growth, increase weight and used as an additive for livestock and aquatic product feed.

The invention discloses a compound biological pesticide and a preparation method thereof. The compound biological pesticide is prepared by components in parts by weight as follows: 0.1 part of lentinan, 1-5 parts of amino acid, 10-20 parts of bupleurum, 10-30 parts of codonopsis pilosula, 10-20 parts of aconitine, 15-20 parts of radix euphorbiae lantu, 15-25 parts of ephedra and 1-5 parts of a suspending aid. Compared with ordinary chemical pesticide in the prior art, the prepared compound biological pesticide has the characteristics of good effect and low toxicity and can be used as an ideal environment-friendly agricultural plant protection drug; meanwhile, two or more pesticides are compounded, so that on one hand, the bactericidal and insecticidal spectrum can be widened, and on the other hand, the pesticide effect can be improved; and more importantly, the problem of drug resistance due to the fact that a single pesticide is used for a long term is solved.