Pymetrozine-resistant isaria javanica and application thereof

A technology of I. clavulata and pymetrozine, applied in the field of biological control, to solve the problem of drug resistance and pesticide residue, and reduce the amount of use

Active Publication Date: 2018-06-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no pests resistant to insect pathogenic fungi have been found, so the application and development of entomopathogenic fungi have great potential in controlling the problem of insecticide resistance in pests

Method used

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  • Pymetrozine-resistant isaria javanica and application thereof
  • Pymetrozine-resistant isaria javanica and application thereof
  • Pymetrozine-resistant isaria javanica and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and identification of I. javanica

[0027] 1. Source of materials

[0028] (1) The carcasses of the brown planthopper [Nilaparvata lugens (Stal)] infected by the fungus were collected from the rice in Guangzhou area.

[0029] (2) Czapek medium (Czapek): 30.0g sucrose, ammonium nitrate (NH 4 NO 3 ) 3.0g, potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0g, magnesium sulfate (MgSO 4 .7H 2 O) 0.5g, potassium chloride (KCl) 0.5g, ferrous sulfate (FeSO 4 ) 0.01g, 20g of agar powder into a beaker, add water to heat and dissolve to make the total volume 1000ml, and then sterilize by autoclave (121°C, 20min).

[0030] (3) Aseptic operation conditions: All utensils and utensils must be sterilized by autoclaving (121°C, 30min), and operations such as inoculation are carried out in an ultra-clean workbench.

[0031] (4) Culture conditions: culture in an incubator at 25±1°C under light (14L:10D). After the colonies are formed, transfer them to the slant of ...

Embodiment 2

[0043] Example 2 Screening of excellent strains of I. javanica

[0044] I. javanica is diverse in terms of genetics, ecology, and biology. Different strains of the same fungus have significant differences in pathogenicity to target pests. Different strains have different LD 50 , LT 50 The difference can be several times to tens of times. The screening and acquisition of high-yielding and high-quality strains is the first prerequisite for achieving better control effects when controlling pests. Based on the three commonly used indicators of pathogenicity, spore production, and spore germination rate, the excellent strains of I. javanica were screened.

[0045] 1. Treatment of test strains

[0046] The 8 isolates of I. javanica A, B, C, D, E, F, G, and H obtained after purification were inoculated on the Chase medium plate, and placed in an incubator at 25±1°C (14L: 10D ) cultured.

[0047] 2. Tested insects and host plants

[0048] For brown planthoppers, inoculate the bro...

Embodiment 3

[0070] Example 3 Determination of pathogenicity of I. javanica strain IJNL-N8 to brown planthopper

[0071] 1. Bioassay is one of the effective means to detect the lethality and lethality rate of entomogenic fungi to target pests, and it can provide an important reference for comprehensive evaluation of the biological control potential of fungi. In this study, the pathogenicity of I. javanica IJNL-N8 strain to brown planthopper was carried out to screen out the optimal concentration for control.

[0072] (1) Tested insects and host plants: brown planthopper, inoculate the adult brown planthopper raised on rice in the net room to insect-free rice plants, and place them in insect cages (60×60×60cm) at 25±1°C , to be used when the brown planthopper on the rice plant develops to the 3rd, 4th, and 5th instar nymphs. The rice is a conventional cultivar, provided by Mr. Liu Yongzhu of South China Agricultural University, and the potted seedlings higher than 30 cm after tillering wer...

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Abstract

The invention discloses a pymetrozine-resistant isaria javanica IJNL-N8 strain and application thereof. The strain is preserved in CCTCC (China Center For Type Culture Collection) on November 22, 2017, and the collection number is CCTCC NO:M 2017709. According to the invention, the isaria javanica IJNL-N8 strain with high pathogenicity to rice planthopper is obtained by screening, and by virtue oflong-term infection biology and indoor biological assay, results show that the strain has a very high insecticidal effect on rice planthopper. The isaria javanica IJNL-N8 strain is a biological control fungus, serves as a living biological insecticide, has bran-new action mechanisms different from existing chemical insecticides, does not pollute the environment, has no residue, can solve the problems of drug resistance and pesticide residue in prevention and treatment processes of rice planthopper, meets the requirements for production of organic food, and has excellent application potentialin biological prevention and treatment of rice pests.

Description

technical field [0001] The invention belongs to the technical field of biological control, and more specifically relates to a pymetrozine-resistant I. javanica and application thereof. Background technique [0002] Rice planthoppers, including brown planthopper and white-backed planthopper, are important pests of rice. They are widely distributed in my country. When brown planthoppers and white-backed planthoppers and other pests seriously occur in southern rice-producing areas, they can directly lead to large-scale rice yield reduction. Traditional chemical pesticides such as thiamethoxam, imidacloprid, and pymetrozine are often used for prevention and control. Long-term and large-scale use of chemical insecticides has led to varying degrees of resistance to rice pests such as brown planthopper and white-backed planthopper. Pesticides kill natural enemies, destroy the ecological environment of rice fields, and weaken the self-regulation ability of the ecological environment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 黄振
Owner SOUTH CHINA AGRI UNIV
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