Thuringiensis bacillus strain for killing noctuidae pests and its preparing process

Abstract

A Thuringiensis bacillus for killing moctuidae pests is prepared through loading soil sample in test tube containing nutritive liquid, vibration culture, diluting, coating on discrete culture medium, inversion culutre, choosing the monoclony able to generate parasporal crystal, purifying, inoculating to fermenting culture medium, shake-flask fermentation until parasporal crystal is dropped, and mixing the centrifugal deposit, supernatant of fermented liquid and raw fermented liquid with artificial feed respectively. It has strong pesticiding toxin.

Description

technical field

[0001] The invention relates to a Bacillus thuringiensis strain with double toxicity (parasporal crystal and exotoxin protein) to efficiently kill Noctuidae pests. Bacillus thuringiensis (Bacillus thuringiensis) DL-5789 strain has a strong poisonous effect on noctuidae pests such as cotton bollworm, Spodoptera litura and Beet Spodoptera due to the composite effect of parasporal crystals and exotoxin protein. It also relates to the preparation method of the bacterial strain. Background technique

[0002] Bacillus thuringiensis (abbreviated as BT) has been used in large-scale production worldwide, and is widely used in the control of agricultural pests, forestry pests, storage pests and sanitation pests. It is an effective microbial insecticide in the world and plays an extremely important role in the practice of microbial control of pests. However, the currently commercialized BT insecticides are mainly used to control sensitive pest...

Examples

Embodiment Construction

[0021] 1. Strain isolation and cultivation

[0022] Put a little soil sample into a test tube containing 4.5ml of nutrient solution, and culture it with shaking for 48 hours at 78°C in a water bath for 15 minutes. Dilute the culture medium to 10 -7 -10 -8 times, spread it on the separation medium, culture it upside down in a 30°C incubator for 3 days, and select a single colony producing paraspora crystals by microscopic examination.

[0023] Nutrient medium: peptone 1% beef extract 0.3% NaCl 0.5% pH7.5.

[0024] Separation medium: peptone 1% beef extract 0.3% NaCl 0.5% agar 2% PH7.5.

[0025] 2. Strain shake flask fermentation

technical field

[0001] The invention relates to a Bacillus thuringiensis strain with double toxicity (parasporal crystal and exotoxin protein) to efficiently kill Noctuidae pests. Bacillus thuringiensis (Bacillus thuringiensis) DL-5789 strain has a strong poisonous effect on noctuidae pests such as cotton bollworm, Spodoptera litura and Beet Spodoptera due to the composite effect of parasporal crystals and exotoxin protein. It also relates to the preparation method of the bacterial strain. Background technique

[0002] Bacillus thuringiensis (abbreviated as BT) has been used in large-scale production worldwide, and is widely used in the control of agricultural pests, forestry pests, storage pests and sanitation pests. It is an effective microbial insecticide in the world and plays an extremely important role in the practice of microbial control of pests. However, the currently commercialized BT insecticides are mainly used to control sensitive pest...

Examples

Embodiment Construction

[0021] 1. Strain isolation and cultivation

[0022] Put a little soil sample into a test tube containing 4.5ml of nutrient solution, and culture it with shaking for 48 hours at 78°C in a water bath for 15 minutes. Dilute the culture medium to 10 -7 -10 -8 times, spread it on the separation medium, culture it upside down in a 30°C incubator for 3 days, and select a single colony producing paraspora crystals by microscopic examination.

[0023] Nutrient medium: peptone 1% beef extract 0.3% NaCl 0.5% pH7.5.

[0024] Separation medium: peptone 1% beef extract 0.3% NaCl 0.5% agar 2% PH7.5.

[0025] 2. Strain shake flask fermentation