382results about How to "Pest control" patented technology

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

The invention discloses an organic fertilizer fermented by chicken manure and a production method of the organic fertilizer, which belong to the technical field of culture waste treatment and biological fertilizer manufacture. According to the invention, the organic fertilizer is prepared and produced by the steps of aerobic curing and anaerobic fermentation, so that the problems of pollution and root burning caused by the chicken manure directly applied to fields are solved, and the innocent treatment and resource utilization of the chicken manure and padding are realized. The final product mainly comprise beneficial bacteria and metabolites of the beneficial bacteria, which have the characteristics that the fertility is durable, the macroecology of the soil can be improved to inhibit pest and disease damage, the soil structure can be improved to improve breathability and the development of the root systems of plants can be enhanced. The purpose of the aerobic curing is to inhibit the growth of infectious microbes, control metabolism direction, kill pathogenic microorganisms, adult parasite, parasite eggs and thermolabile infectious microbes by biological heat and provide a matrix for anaerobic fermentation by decomposing, transforming and synthesizing the microorganisms into thallus ingredients and small molecular substances; and the purpose of the anaerobic fermentation is to augment the beneficial bacteria and generate the metabolites such as organic acid.

The invention relates to a biogas residue organic fertilizer and a preparation method thereof. The biogas residue organic fertilizer uses biogas residue as major raw materials (the consumption is higher than 80 percent) to be mixed with humic acid, nitrogenous fertilizers, phosphate fertilizers, potash fertilizers and trace elements. By aiming at two problems that the biogas residue can not be dewatered and solidified easily and the mechanical dewatering has high energy consumption, the invention utilizes a shallow tank natural solidifying and air drying process, and the biogas residue carries out aerobic secondary fermentation, air drying and solidification in a shallow tank to meet the pelletization moisture and physical property requirements. The biogas residue organic fertilizer of the invention can meet the requirement of nutrient required by crop growth, and can also fertilize the soil and improve the yield.

The invention discloses a constant temperature and humidity storage box of gingers, which belongs to article storage boxes. The structure of the constant temperature and humidity storage box of the gingers comprises a box body, a box door, a refrigerating unit, a control panel, a microcomputer control circuit, an ultraviolet sterilizing lamp, an electric heating pipe and an evaporator, wherein the back part and bottom of the box body are respectively provided with a back part partition board and a bottom partition board; an air inlet fan and an air exhaust fan are respectively arranged at the top of the back part partition board; a ventilating hole is formed on the bottom partition board; a water tank is formed at the lower part of the evaporator; a water tank outer cover is arranged on the outer wall of the box body corresponding to the water tank; a ventilating cover is arranged in the middle of the top of the box body; the ultraviolet sterilizing lamp is arranged on the box top in the interior of the box body; a temperature and humidity sensor is arranged on the side wall of the middle part of the box body; and the microcomputer control circuit is electrically connected with the control panel, the temperature and humidity sensor, the refrigerating unit, the air inlet fan, the electric heating pipe, the air exhaust fan, the ultraviolet sterilizing lamp and the evaporator respectively. The constant temperature and humidity storage box of the gingers has the characteristics of good ventilating and heat radiation condition and no generation of stuffiness and decayed gingers; and the temperature and humidity as well as plant diseases and insect pests can be effectively controlled.

The invention discloses special bio-organic fertilizer for organic tea and a preparation method of the special bio-organic fertilizer, and solves the problem of lack of appropriate and special fertilizer for organic tea planting in Guizhou province. The special bio-organic fertilizer for organic tea comprises the following components in percentage by weight: 25-35 percent of distiller grains, 15-25 percent of Chinese herb residue, 30-40 percent of chicken manure, 4-6 percent of mineral substance powder, 0.01-0.03 percent of a microbial agent I, 10-20 percent of a conditioner and 0.1-5 percent of a microbial agent II. The invention further provides the preparation method of the special bio-organic fertilizer for organic tea. According to the invention, abundant organic waste resources of Guizhou province are utilized, various nutrients are provided for the growth of organic tea, the improvement on the soil structure and the fertilizer utilization rate is facilitated, and the yield and the quality can be increased remarkably; and meanwhile, the cyclic utilization of organic waste is realized, the fertilizer preparation cost is low, and favorable economic benefits and ecological environment benefits are achieved.

The invention discloses an ecological organic liquid fertilizer which comprises components in parts by weight as follows; 70-80 parts of vitex negundo leaves, 30-40 parts of green manure, 10-20 parts of pyrethrum, 20-40 parts of molasses, 0.4-0.6 parts of choline chloride, 0.1-2 parts of an antibacterial peptide, 1-3 parts of microelements, 1-5 parts of an amino acid, 1-4 parts of biological bacteria, 2-3 parts of a dispersing agent, 3-10 parts of water-retaining agent and 100-150 parts of water. The ecological organic liquid fertilizer has the advantages of low cost, high fertilizer efficiency, high comprehensive effect, capability of controlling diseases and pests, reducing environmental pollution and improving the soil and the like.

The invention provides a method for preparing an EM bio-organic fertilizer by use of biogass slurry. The method comprises the following steps: adding an EM stock solution and an EM nutrient fluid into biogass slurry, and cultivating the biogas slurry by EM to obtain a bio-organic fertilizer, wherein the mass ratio of biogas slurry, the EM stock solution and the EM nutrient fluid is (95-105): (2-6): (0.5-1.5). As the EM cultivation mode is adopted, the method for preparing the EM bio-organic fertilizer by use of biogass slurry has the advantages of being convenient, simple, easy to operate and the like. The bio-organic fertilizer contains rich organic matters and lots of beneficial bio-funguses, can play a soil improvement role, can accelerate decomposition of the organic matters, has the advantages of being rich in nutrition, being easy to be absorbed by plants, improving the yield and the quality of crops and the like, is a leaf fertilizer with comprehensive nutrients and can treat and control some plant diseases and insect pests of the crops.

Disclosed herein are pest controlling compositions (i.e., biopesticides) comprising one or more entomopathogenic fungi which. Further disclosed are methods of using such compositions for controlling invasive pests, particularly agriculturally relevant pests.

The invention relates to a photolysis-resistant pesticide and application thereof. The photolysis-resistant pesticide comprises the following components in percentages in weight: 0.1-90 percent photosensitive pesticide active ingredient, 0.2-30 percent water-soluble ultraviolet absorbent, 0.2-20 percent oil-soluble ultraviolet absorbent, 0.2-20 percent antioxidant, 1-20 percent surfactant and 4-95 percent solvent or carrier. According to the photolysis-resistant pesticide, the water-soluble ultraviolet absorbent and the oil-soluble ultraviolet absorbent are adopted for synergistic effect, and simultaneously the antioxidant is added; and therefore, at least 30 percent, even more than 50 percent, ultraviolet light can be absorbed, the photolysis-resistance of the photosensitive pesticide active ingredient can be improved, and the half-life of the pesticide can be prolonged; namely, the exposure durations of pests (bacteria, weeds or mites and the like) under the pesticide atmosphere are increased, the using effect of the pesticide is enhanced, and the use amount of the pesticide is reduced; and the photolysis-resistant pesticide can be used for preventing and controlling various pests and diseases of plants, especially crops.

The invention discloses a preparation method for a special amino acid chelating liquid-containing composite coated base fertilizer for rape. The preparation method comprises the following steps: uniformly mixing 25 to 30 parts by weight of urea, 10 to 12 parts by weight of diammonium phosphate, 10 to 15 parts by weight of calcium superphosphate, 18 to 22 parts by weight of potassium chloride, 12 to 15 parts by weight of magnesium sulfate, 0.1 to 0.2 part by weight of borax, 1 to 3 parts by weight of catnip powder, 1 to 2 parts by weight of diatomite, 3 to 5 parts by weight of compound powder; then spraying 4 to 6 parts of amino acid chelating liquid and 4 to 5 parts by weight of bonding liquid and carrying out granulation so as to obtain composite fertilizer particles; and sprinkling 8 to 10 parts by weight of molten paraffin on the surface of 100 parts by weight of the composite fertilizer particles to realize coating so as to obtain the special composite coated base fertilizer for rape. According to the invention, a variety of nutrient elements needed in growth of the rape are reasonably compounded to prepare the fertilizer, and the fertilizer has the advantages of a light weight and excellent water retention, fertilizer retention and slow release performance.

The invention belongs to the technical field of fruit tree planting, and particularly relates to a citrus gonggan cultivation and management method. According to the growth cycle, the method comprises the following steps of spraying plenty of leaf fertilizer, and applying base fertilizer deep during the dormancy stage and flower bud differentiation stage from December to January; during the spring-shoot germination stage in February, spraying germination fertilizer; during the bud stage, early flowering stage and spring-shoot growth stage in March, supplementing the flowering nutrition; during full-bloom stage, bloom-fall stage and physiological fruit-drop stage in April, spraying fruit protecting fertilizer and fruit stabilizing fertilizer; during the summer bud germination stage and physiological fruit-drop stage in May, spraying the leaf fertilizer for fruit protection; in June, spraying fruit expanding fertilizer; in July, heavy dressing autumn growth fertilizer; during the autumn growth stage and fruit expanding stage in August, adding the autumn growth fertilizer; in September, top dressing quick-acting fertilizer and outroot feeding; in October, keeping the soil little dry; in November, spraying the leaf fertilizer; in December fruit stage picking, stopping irrigation 10 days before picking. The method enables the tree growth potential to be strong, effectively controls insect damage, prolongs the service life of nursery stocks, increases the production of the fruit, reduces the investment ratio and can improve greatly the economic benefits of fruit growers.

The invention discloses an intercropping planting method of corn plants, tomato plants and pole bean plants and belongs to the field of crop cultivation methods. The corn plants and the tomato plants are intercropped every other ridge first, the width of the broad ridge is 80-90cm, the corn plants are planted with the row space of 40-45 cm and the plant space of 30-35cm, the width of the narrow ridge is 60-70 cm, and the tomato plants are planted with the row space of 30-35cm and the plant space of 30-35cm. The pole bean plants and the corn plants, and the pole bean plants and the tomato plants are intercropped within rows, and one pole bean plant is planted between every two corn plants and between every two tomato plants. The planting proportion of the corn plants, the tomato plants and the pole bean plants is 1:1:2. When the tomato plants grow to the height of 35-40cm, steel supports are arranged between two lines of the tomato plants of the narrow ridge and 1.0m above the ground, the supports support the tomato plants and the pole bean plants, and the supports used for supporting the pole bean plants and the costs are saved. The planting method fully takes advantage of the land and space resource, the three crops are well-proportioned and interact with one another, the leguminous plants fix nitrogen, the chemical fertilizers and the pesticides are saved, the planting cost is saved, the pressure of the nitrogenous fertilizer overuse to the environment is reduced, and the yield is increased on the limited land.

The invention relates to a cultivation technique for three high and one safe of double harvest rice, comprising the following steps: before sowing, sprout cultivation fertilizer of the invention is used as basic fertilizer and spread at one time, the usage amount is 30-75g/m<2> seedling bed, early rice with 3-4 leaf age and late rice with 5-6 leaf age are transplanted to big field at proper time; a method of broad row spacing and narrow planting distance is adopted, the row spacing is 27-30cm, and the planting distance is 13-17cm; 12-14 kilograms of chemical nitrogenous fertilizer is spread on each mu in the planting, when the number of plant reaches 70%-90% of the planned spike number, drainage and field drying are carried out, then slight drying is carried out for three to four times, water is regained in the leaf sprouting period of the second leave from the top; rice tillering-control agent containing gibberellin is spread in the critical leaf age period of the effective tillering; and the agent is spread on the leaf surface in the crevasse period and in the milk time. The seedling-strengthening and premature-promoting technique of the invention, by utilizing rice sprout cultivation fertilizer for cultivating, simplifies the sprout cultivation procedure, and can prevent and treat plant diseases and insect pests in seedling bed, cultivate dumpy sprouts and promote the premature of big field.

The invention provides a plant nutrient solution. The plant nutrient solution is made from, by weight, 8-12 parts of brown sugar, 60-80 parts of water, 0.1-0.2 part of multienzyme compound enzymes and 0.4-0.6 part of bran. The multienzyme compound enzymes are formed by mixing photosynthetic bacteria, lactic acid bacteria, saccharomycetes, filamentous bacteria, heat-resisting bacilli and phosphorus and potassium decomposition nitrogen-fixing bacteria according to the weight ratio of 1 to 1 to 1 to 1to 1 to 1. The plant nutrient solution has the advantages that the plant nutrient solution is used for soaking seeds and cuttings and can increase plant germination rate and survival rate; the plant nutrient solution is used for soil erosion and capable of activating soil, promoting breeding of beneficial microorganisms in soil, plays an important role in converting soil organic matter, increasing the contents of effective nitrogen, phosphorus and potassium in soil and increasing soil fertility and plays an import role in development of a plant root system.

The invention includes an expression vector engineered to produce double-stranded RNA (dsRNA) within a pest to be controlled. The dsRNA inhibits expression of at least one gene within the pest, wherein inhibition of the gene exerts a deleterious effect upon the pest. For example, inhibition of the gene can lead to cessation of feeding, growth, or development and can cause death of the pest. In a preferred embodiment of the invention the expression vector is a recombinant baculovirus that transcribes sense and antisense RNA under the control of the baculovirus IE-1 promoter and hr5 enhancer. Preferred genes to be inhibited include essential genes, genes involved in neurotransmission, and genes that are targets for conventional pesticides. The invention discloses baculovirus transfer plasmids useful for producing the recombinant baculovirus. The invention further discloses methods and formulations involving the expression vector.

The invention discloses a corn planting method. The method comprises the steps of base fertilizer application, seed soaking and sowing, transplanting, field management, harvesting and the like, Chinese herbal liquid medicine is adopted for soaking seeds, and a variety of insecticide is adopted for cooperating in sterilization, so that young seedlings and fully-grown seedlings are great in disease resistance, soil is good in drainage capability and water retention capability, corn growth is facilitated, Chinese herbal medicine is utilized for replacing chemical insecticide, environmental pollution is reduced, corn plants which are planted and finally obtained are high in acre yield and excellent in quality, and economic benefits are remarkable.

The invention relates to the technical field of agricultural planting, particularly a planting method for dendrobium officinale. The method comprises the steps of: preparing a seedbed; preparing a cultivation matrix; preparing flower pots; performing fixed planting; performing watering; performing fertilization; performing pest control; controlling temperature and humidity and the like. The planting method provided by the invention has the beneficial effects that the survival rate of transplanted tissue culture seedlings reaches over 99%; by virtue of spraying biogas slurry, particular biogas slurry produced in a soft biogas pool, performing special treatment on a cultivation matrix, fermenting silkworm feces as top dressing and self-making a natural plant bactericide to prevent and treat plant diseases and insect pests, the produced dendrobium officinale is fleshy in leaf and stem, high in various nutritional components, quick to grow, high in yield and higher than wild dendrobium officinale in effective medicinal component content, and the occurrence rate of plant diseases and insect pests is obviously reduced, so that the cost is lowered, and the food safety of dendrobium officinale is improved. By adopting the method to plant dendrobium officinale, harvest within 12-14 months can be realized.

The invention discloses a pollution-free cultivation method for isatis root. The method mainly comprises the steps of soil preparation, sowing, thinning and final singling, growth period management, disease and pest control and harvesting. The method provided by the invention adopts a traditional Chinese medicinal pesticide, so the growth environment of isatis root is purified, diseases and pests of isatis root are effectively prevented and treated, and the problems of high residual of chemical pesticides, high pollution and poor insect-preventing effects in the prior art are overcome. In addition, in the process of planting, a great amount of farmyard manure is used as main fertilizer, so the medicinal properties of isatis root are protected to a greatest extent; the yield and the quality of isatis root are effectively improved; and the harvested isatis root has long, straight, strong and uniform roots. The cultivation method is advanced and reasonable, has strong operability, can be learned and used by all kinds of populations and has good popularizing value.