214 results about "Candida sp" patented technology

A preparation technique of chitosan microspheres immobilization lipase comprises the steps that: (1) lipase from candida SP is dissolved in phosphate buffer solution by taking chitosan microspheres as an immobilization carrier and adopting glutaraldehyde cross-linking, lipase A is obtained. (2) candida SP lipase solution of 0.70-0.80mg/mL prepared by phosphate buffer solution b with a pH value of 6.5-7.5 is added with immobilization lipase A (final concentration is 0.005-0.0055g/ml) and after being stirred, ethyl (3-dimethylamino propyl) carbodiimide hydrochloride solution is added, the mixture is then stirred to be fully immobilized, chitosan microspheres immobilization lipase B is obtained after the treatment of the mixture. The chitosan microspheres immobilization lipase prepared by the preparation technology of the invention improves apoenzyme combination rate and activity recovery, has powerful immobilization lipase activity and specific activity and the immobilization lipase can be recycled for six times.

A bioprocess for producing carboxylic acids, alcohols and aldehydes is provided by culturing Candida sp. in a fermentation medium containing various defined substrates.

The invention relates to a preparation method and application of a compound leavening agent. The preparation method comprises the steps of: separating lactobacillus plantarum, lactobacillus bulgaricus, streptococcus thermophilus, saccharomyces cerevisiae, candida rugosa, candida tropicalis and fermented pichia pastoris, as a leavening agent, from naturally fermented panada to produce a strain so as to prepare freeze-dried strain powder; weighing 1-2 parts of lactobacillus plantarum, 1-2 parts of lactobacillus bulgaricus, 1-2 parts of streptococcus thermophilus, 3-5 parts of saccharomyces cerevisiae, 3-5 parts of candida rugosa, 3-5 parts of candida tropicalis and 3-5 parts of fermented pichia pastoris based on parts by weight, and uniformly mixing to obtain the compound leavening agent. When the compound leavening agent is applied to the cooked wheaten food, bacterial contamination can be effectively prevented, the sanitation of the cooked wheaten food is guaranteed, the quality of the cooked wheaten food is improved, the production cycle is shortened, a production process is controllable, and the preparation method of the compound leavening agent lays a foundation for industrial production.

The invention provides a compound bacterial agent for purifying a black and odorous water body and a preparation method thereof. The compound bacterial agent comprises the following components: actinomycetes, bacillus subtilis, nitrifying bacteria, lactic acid bacteria, candida utilis and pseudomonas aeruginosa in a weight ratio of 2:2:2:1:1:1. The invention has the following advantages: the COD content, ammonia nitrogen content and phosphate salt in the black and odorous water body can be effectively reduced, and the odor of the black and odorous water body can be eliminated; and meanwhile, by adding the ammonia nitrogen removal strain, the transparency of the water body can be gradually improved.

The invention relates to a production process for bioorganic fertilizer with high bacteria content. The production process comprises the following steps: separately activating a trichoderma viride strain and a trichoderma pseudokoningii strain; separately activating a bacillus amyloliquefaciens strain, a bacillus subtilis strain and a candida utilis strain; carrying out step-by-step enlarged culture and the like. A preparation method provided by the invention has the advantages of low cost, simple process, high operability and convenience in popularization and application; the obtained bioorganic fertilizer is high in content of effective living bacteria; a large amount of corn stalk resources can be really and efficiently converted, soil is remediated, the fertility of the soil is improved, and wastes are changed into valuables; in addition, the problem of environmental pollution caused by straw burning can be relived to a certain degree.

The invention relates to the technical field of biology, and relates to a lipase mutant and uses thereof. The mutant is obtained from wild type candida antarctica lipase B (CALB) through mutation. The invention particularly relates to the lipase mutant, a preparing method thereof, and a method of preparing S-configuration ibuprofen type compounds with optical activity through catalysis of hydrolysis of ester derivatives of the ibuprofen type compounds by utilization of the lipase mutant and through kinetic resolution. The wild type candida antarctica lipase B is subjected to mutation to obtain the modified lipase. The lipase is expressed in pichia pastoris engineering bacteria. The lipase comprises an amino acid sequence having at least 85% of identity with the SEQ ID NO.02. The 189 site residue corresponding to the SEQ ID NO.02 of the lipase is an aromatic amino acid residue and the 190 site residue corresponding to the SEQ ID NO.02 of the lipase is a nonpolar amino acid residue. By subjecting the wild type candida antarctica lipase B to mutation, the enantioselectivity of the lipase to the ibuprofen type compounds is improved.

The invention provides a preparation method of a soil ecological restoration agent for solving old ginseng land continuous cropping obstacle. The preparation method includes the steps: (1) mixing 20-40 parts of bacillus licheniformis, 10-20 parts of candida utilis, 10-30 parts of lactobacillus plantarum, 10-20 parts of trichoderma viride and 5-10 parts of rhodopseudomonas palustris according to the weight ratio to obtain compound bacteria; (2) adding the compound bacteria to a compound culture medium according to the weight ratio of 1:(80-120), stirring evenly, then culturing under a condition of the temperature of 28-32 DEG C until the pH is less than 4.00, then carrying out closed culture for 12-15 days, and thus obtaining the soil ecological restoration agent in a liquid form; and furthermore, making the liquid form into a solid form. Through the production of the microbial inoculant, starting from restoration of a soil ecological system, and with use of the multi-strain combined technology method, beneficial bacteria in soil and beneficial nutritional factors for promoting the growth of ginseng are increased, and harmful bacteria generated by continuous cropping are inhibited.

The invention aims at providing a compound microecological preparation for livestock and poultry excrement treatment and a preparation method and application thereof. For 100 g of livestock and poultry excrement, the compound microecological preparation needed to be added is prepared from, by volume, lactobacillus casei 0.03-0.10 Ml, enterococcus faecalis 0.03-0.10 mL, candida mycoderma bacteria 0.05-0.50 mL and bacillus subtilis 0.03-0.10 mL, wherein effective living bacterium number of bacterium liquid of the lactobacillus casei, the enterococcus faecalis, the candida mycoderma bacteria and the bacillus subtilis is 1.0 * 109 cfu/mL. The compound microecological preparation is utilized for fermentation of livestock and poultry excrement, the pH and the enterococcus faecalis number of the preparation can be reduced (P> 0.05), the content of indole is reduced by 58% (P< 0.05), and the compound microecological preparation has the great significance on fecal odor elimination.

The invention provides a production process of fermented cottonseed meal. The process comprises the following steps: breeding lactobacilli, bifidobacteria, bacillus subtilis and candida utilis by adopting a self-made culture medium at the same time, mixing obtained strain liquid, acid protease, cellulase, aspergillus oryzae and yeast enzyme with cottonseed meal, performing fermentation production, drying, grinding and packaging. According to the fermented cottonseed meal produced by adopting the process, free gossypol in the cottonseed meal can be effectively degraded, the palatability of the cottonseed meal can be improved, macromolecular proteins are decomposed into micromolecular polypeptides or amino acids convenient to absorb by animals, crude proteins and crude fibers are degraded, a large quantity of active probiotics are secreted and synthesized, and the nutritional value of the cottonseed meal as a feed is improved.

The invention relates to a probiotic feed additive for Chinese softshell turtles and a preparation method of the probiotic feed additive. The probiotic feed additive is prepared from probiotics and carrier fillers, wherein the carrier fillers are sterilized wheat bran, and the probiotics include bacillus subtilis, bacillus natto, Lactobacillus casei and candida utilis with the ratio of (0.4-0.6):(0.4-0.6):(0.8-1.2):(0.8-1.2), and the probiotics are added in the aseptic carrier fillers to prepare probiotic powder. The probiotic feed additive can improve the growth performance of the Chinese softshell turtles, reduce the food coefficient, improve the immunological function and reduce the diseases, has the advantages of low cost, no pollution, no drug resistance, no residue and the like, and is environmentally friendly.

The invention relates to low-yield isoamylol and high-yield beta-phenylethanol yeast, an isolated culture method thereof and an application of the yeast. The low-yield isoamylol and high-yield beta-phenylethanol yeast is named as a Candida humilis YS023A strain, the strain is collected in a general microorganism center of a China Committee for Culture Collection of Microorganisms on No. 3, No. 1 courtyard Beichen West Road, Chaoyang District, Beijing on February 25, 2019, and the collection number of the strain is CGMCC NO:17257. The yeast has excellent characteristics of low-yield isoamylol and high-yield beta-phenylethanol and excellent sugar, alcohol and acid resistance, and the sugar utilization rate, the fermentation capacity and the ethanol production capacity of the yeast are good.The yeast is used for production of strong-flavor and rice-flavor Baijiu and has great significance for increasing of high-quality product rate and wine outlet rate of raw wine.

The invention relates to a biological protein feed and a production method thereof. The production method comprises: crushing corn straw into particles capable of being screened with a 20 mesh sieve, and adopting a rail type autoclave to carry out a moisture and heat steam treatment for 30 min at a temperature of 100 DEG C; adopting a two-step method to ferment, wherein Gloeophyllum trabeum is firstly inoculated to ferment, urea, magnesium sulfate and potassium dihydrogen phosphate are added to the obtained primary fermentation product, uniform mixing is performed, and Aspergillus niger, Aspergillus oryzae, Bacillus subtilis and Candida utilis are inoculated to carry out secondary fermentation; drying after completing the fermentation; and carrying out 40 mesh crushing to obtain the biological protein feed. According to the present invention, the biological protein feed production method has characteristics of simple process and low cost; with application of the produced biological protein feed to feed milk cows, the milk yield can be increased by more than 4.7%, and the milk protein content can be increased by more than 4.1%; and the produced biological protein feed is suitable for various types of high, medium and low-end milk cow farming customers, and can further be used for feeding other livestock and poultry including piglets, chickens, ducks, geese and other poultry.

The invention relates to an inoculating culture and detecting method for gathering copper ions through saccharomycete. According to the inoculating culture method, at first, inoculating culture media with different copper ion concentrations and a basal culture medium are prepared; the inoculating culture media with the different copper ion concentrations are inoculated with a cultured candida utilis strain solution, and shaking culture is carried out; candida utilis with a high OD600 value and the low content of the copper ions in supernatant liquor is selected from the cultured strain solution to serve as starting strains; a starting strain solution is taken out, the inoculating culture medium with the smallest copper ion concentration is inoculated with the starting strain solution, after shake-flask culture is carried out, the strain solution is absorbed again, the inoculating culture medium with the second smallest concentration is inoculated with the strain solution, and inoculating culture is carried out according to the method; finally, copper ion conversion rates of different generations of the candida utilis are detected, and when the conversion rates of the copper ions are unchanged or reduced, the strains are strains for producing copper gathering yeast. The cultured saccharomycete can grow in the culture medium with the large copper ion concentration, the conversion efficiency of the copper ions is high, and the basis is provided for development of the new function of the yeast.

The invention relates to the technical field of feed additive and processing thereof, and in particular to a probiotic feed additive. The probiotic feed additive is prepared by mixing autonomously separated lactobacillus plantarum, candida rugosa, candida ethanolica and lactococcus garvieae and buffalo milk fermentation substrate to ferment. The preparation process disclosed by the invention is simple, and the probiotic feed additive can be widely applied in the raising of livestock and poultry such as milk buffalos, pigs, chickens, cattle and sheep. The probiotic feed additive can remarkably improve the intestinal microbial floras of animals and enhance immunity, and can reduce the usage of antibiotic medicines and eliminate medicine residue, and when applied in production, the probiotic feed additive can shorten feeding periods and save feeds, thus increasing farming income.

The invention relates to a method for preparing a novel biological microcapsule capable of being applied to treatment of ammonia nitrogen and soluble organic substances in water. According to the method for preparing the microcapsule, no chemical crosslinking agent or external material is added as a carrier, but candida is spontaneously combined to an extracellular polymer excreted by fungal hyphae cell aggregation in a natural mode under a special condition, and an appropriate condition is created to induce the intergrowth of hyphomycete and the candida so as to form the biological microcapsule in the shape of a solid microsphere. The novel biological microcapsule can efficiently degrade the ammonia nitrogen and the soluble organic substances in water, can keep the activity of thalli, separates the thalli from the outside poor environment, slows down the influence caused by temperature and pressure, and is favorable for uniform distribution in water, and also favorable for stability in storage and use. The preparation method is simple and feasible, has less influence factors, and has great potential and application value for treating the ammonia nitrogen and the soluble organic substances in the water.

The invention discloses a preparation method of a magnetic biological microcapsule for processing organic matters and ammonia nitrogen in water. The method comprises the following steps: 1, adding Candida yeast living cells into a disinfected YPD culture medium, culturing at 25-28DEG C under 150-200rpm for 5-6h, taking a bacterial strain liquid obtained after a logarithmic phase, carrying out low temperature drying refrigeration, centrifuging, washing, and carrying out suspension extraction by using normal saline to obtain a bacterial strain Candida yeast suspension with the OD value of 1.5-1.8; 2, mixing sodium alginate and Fe3O4 powder, adding the obtained mixture to deionized water according to the addition amount of sodium alginate of 19-21g/L and the addition amount of the Fe3O4 powder of 1.8-2.2g/L, heating until sodium alginate is completely dissolved, and cooling; and 3, uniformly mixing the yeast suspension with the obtained sodium alginate-iron powder solution according to a volume ratio of 1:9-1:11, adding the obtained solution mixture into a calcium chloride solution in a dropwise manner, curing for 30-40min, and filtering to obtain the microcapsule. The magnetic biological microcapsule prepared in the invention can efficiently degrade the organic matters and ammonia nitrogen in water, can maintain the vitality of thalli, separates the thalli from extraneous adverse environment, can be uniformly distributed in water and separated from water, and is stable in the storage and use process.

The invention relates to a novel method for extracting fulvic acids from industrially-produced waste edible fungi residues by fermentation. The novel method includes the steps of (1) preparing a primary seed solution; (2) preparing a secondary seed solution; (3) inoculating the secondary seed solution, bacillus subtilis, candida tropicalis and trichoderma pseudokoningii into a 1000-2000L of fermentation tank respectively and fermenting for 10-15 days so as to obtain a fermented seed solution; (4) treating pleurotus ostreatus residues; (5) inoculating the fermented seed solution into the pleurotus ostreatus residues to be inculated, and fermenting at the temperature 20-40 DEG C aerobically for 4-5 days so as to obtain fermented fungi residues; (6) extracting the fulvic acids; (7) performing spray drying so as to obtain flavescent fulvic acid powder. The fulvic acid content can be above 35% after fermentation, and yield of crude fulvic acids can be up to about 30% (dry matter percentage) through extraction.

The invention discloses a composite microbial agent for degrading kitchen waste and a preparation method thereof. The preparation method comprises the following steps: respectively inoculating bacillus subtilis, bacillus licheniformis and bacillus coagulans into a beef extract peptone culture medium for culturing; respectively inoculating candida utilis and plant lactobacillus into a potato glucose culture medium for culturing; and performing mixed fermentation on the bacillus subtilis, bacillus licheniformis, bacillus coagulans, candida utilis and plant lactobacillus to prepare the compositemicrobial agent. By researching compounding of multiple microbial agents, the synergistic effect among strains in a proper proportion is discovered, and main components (cellulose, grease, starch andprotein) of the kitchen waste can be effectively degraded, so that the kitchen waste can be fully fermented. Moreover, the preparation process provided by the invention is simple, and the production benefits are high.

The invention discloses a strain for producing 3-hydroxyl propionic acid at high yield, which is a compound mutant strain of Candida sp., is stable in hereditary features and yield, is applicable to industrial popularization, and is collected in the China general microbiological culture collection center with the collection number of CGMCC No.5344. The invention further discloses a method for converting propionic acid into 3-hydroxyl propionic acid. The method comprises the following steps of when the growth of the strain reaches a stable stage, supplementing glucose in a limited way till theconcentration of the strain is 15-25g/L when the growth of the strain reaches a stable stage; controlling the concentration OD600 of the strain is 18-20; adding propionic acid, potassium hydroxide, strong sodium oxide and ammonia water (according to a ratio of 8: 1: 1: 1) in a flowing way, so as to keep the concentration of the propionic acid at 10-20g/L; and fermenting and culturing for 96 hoursto obtain a fermentation liquid of 3-hydroxyl propionic acid. After optimization, the yield of 3-hydroxyl propionic acid on the culture medium is increased to 30g/L.

Belonging to the technical field of microbial degradation, the invention specifically discloses a microbial compound inoculant for efficient degradation of grease, a method and application thereof. The microbial compound inoculant comprises the following strains bacillus licheniformis, bacillus subtilis, proteolytic candida and candida in a mass ratio of 5-10:10-20:10-15:5-20. After inoculation ofthe microbial compound inoculant provided by the invention into oily sewage at an inoculation amount of 0.5-10% for reaction for 3 days, the grease degradation rate can reach 90% or more. The microbial compound inoculant provided by the invention can be applied to oily wastewater treatment and harmless treatment of culture wastes for resource utilization, can achieve large-scale industrial application, and has the advantages of small investment, high efficiency, no secondary pollution, etc.