62 results about "Candida sp" patented technology

The invention belongs to the technical field of environmental protection of bioremediation wastewater, and discloses an immobilized adsorption microsphere, which is prepared from Bacillus spp., Candida utilis and European nitrosomonas through an immobilized embedding technology. The microspheres provided by the invention can effectively repair glutamic acid high ammonia nitrogen fermentation wastewater.

The invention discloses a method for preparing acid sophorolipid, and belongs to the technical field of biological fermentation. According to the method, candida is used as a fermentation strain, a composite carbon source culture medium containing a water-soluble carbon source and an oil-soluble carbon source is adopted, and the content of residual oil is monitored in real time in the fermentation process; when the residual oil content exceeds a threshold value of 5g / L, the emulsifying agent of 0.1 g / L-0. 5g / L is supplemented, and the stirring rotating speed is increased by 20-50rpm, so that the emulsification of an oil phase and the utilization of the thalli on the oil-soluble carbon source are promoted. By accurately controlling the residual oil content in the fermentation process, the proportion of the acidic sophorolipid and the carbon source conversion rate are remarkably increased, the proportion of the acidic sophorolipid is increased to 60% from 30% of a traditional process, the carbon source conversion rate is increased to 65% from 40%, the problems that the increasing means of the proportion of the acidic sophorolipid is single, and the carbon source is wasted are effectively solved, and the method is suitable for industrial production. The method has the advantages of simple process, low cost, environmental protection and the like.

The present application relates to the technical field of genetic engineering, and in particular to a high-yield sophorolipid gene knockout engineering bacterium, a construction method thereof and an application, the engineering bacterium taking Bombus bornensis Candida as a starting strain, and using a gF-Hph-gR nucleotide sequence to replace a gene sequence seq2 in a sophorolipid synthesis path for regulating a lactonization reaction ， The gF-Hph-gR nucleotide sequence is SEQ ID No. 1, and the seq2 gene sequence is SEQ ID No. 2. The method provided by the present application uses fusion PCR to construct a seq2 gene knockout box with a resistance screening marker, and the shortest time required is 1 day, which provides a new way for efficiently constructing a sophorolipid production engineering strain. The high-yield sophorolipid gene knockout strain provided by the present application lays a foundation for large-scale industrial production of sophorolipid and meets market demand, and has a wide application prospect, economic value and social benefit.

This invention relates to a protein feed prepared from biorefined baijiu lees and its preparation method, belonging to the fields of bioengineering and feed resource development technology. It includes the following steps: (1) subjecting the baijiu lees to acid pretreatment and dual-enzyme synergistic hydrolysis sequentially to obtain a hydrolysate containing fermentable sugars; (2) adding Candida utilis culture and urea for carbon-nitrogen synergistic liquid fermentation to obtain a Candida utilis viable count ≥4.0 × 10⁻⁶. 8 Fermentation broth with CFU / mL; drying is then performed. This invention pretreats baijiu (Chinese liquor) lees under high pressure with dilute acid, followed by synergistic hydrolysis with two enzymes, and then utilizes *Candida utilis* for carbon-nitrogen synergistic liquid fermentation, achieving efficient utilization of glucose and xylose, with a cell protein yield ≥0.70 g / L / h. This invention realizes the high-value utilization of all components of baijiu lees, providing a process route with industrial-scale production potential for the biorefining of baijiu lees to prepare protein feed.

The present application relates to the field of pharmaceutical chemistry, and specifically discloses a emodin mannich base, a preparation method and application thereof. The emodin mannich base has a structure as shown in formula I, the structure is simple, has strong in-vitro antimicrobial activity, especially high inhibitory activity to staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteus vulgaris, pseudomonas aeruginosa, shigella dysenteriae, salmonella typhimurium and other bacteria, and candida utilis, candida albicans, saccharomyces cerevisiae, monascus and other fungi, and can be used for preparing antibacterial and / or antifungal drugs.

The invention belongs to the technical field of cosmetics, and discloses an avocado fermentation composition as well as a preparation method and application thereof. The composition comprises 1%-10% of avocado fermentation filtrate, 0.1%-2% of alpha-glucan oligosaccharide, 0.1%-2% of a wild chrysanthemum flower extract, 3%-8% of a humectant, 0.01%-0.1% of a chelating agent and the balance of deionized water. Wherein the avocado fermentation filtrate is prepared by compound fermentation of aphid torulopsis and candida and is rich in oligopeptide, free amino acid, lactic acid and polyphenol. According to the invention, the filtrate obtained by fermenting avocado with a specific composite strain is scientifically compounded with prebiotics with definite flora selectivity to construct a multifunctional composition with definite structure, synergistic function and high stability; the composition not only provides direct barrier repairing and moisturizing effects, but also actively guides skin micro-ecology to succession to a healthy steady state through nutrition intervention, so that the skin health level is synchronously improved in the two dimensions of molecules and ecology.

The application provides a composite microbial agent, application of the composite microbial agent and a treatment method of high-concentration organic wastewater. The active component of the composite microbial agent provided in the first aspect of the application comprises two or three of Paecilomyces varioti MM2 strain, Aspergillus fumigatus MM6 strain, Candida rugosa MJ4 strain and Bacillus licheniformis MX8 strain. The composite microbial agent provided in the first aspect of the application can be used for biological decontamination treatment of high-concentration organic wastewater, so as to realize degradation of organic pollutants in the high-concentration organic wastewater and pH value adjustment, thereby avoiding increase of the load of a municipal sewage treatment system.

The invention discloses an hph resistance gene knockout box based on gme5 gene editing, a genetically engineered bacterium and application of the hph resistance gene knockout box and the genetically engineered bacterium. The hph resistance gene knockout box comprises a recombinant DNA construction body with the nucleotide sequence shown as SEQ ID NO: 1, the recombinant DNA construction body replaces an endogenous regulatory factor gme5 gene through homologous recombination in a targeted mode, and the nucleotide sequence of the regulatory factor gme5 gene is shown as SEQ ID NO: 2. According to the application, the bumblebee candida albicans are directionally modified through a genetic engineering strategy, an engineering strain for synthesizing the high-yield lactone type sophorolipid is constructed, and the lactonase activity and fatty acid transport capacity are synergistically enhanced, so that the ratio of the lactone type sophorolipid is maximized, and the yield of the lactone type sophorolipid is increased. In the sophorolipid produced by using the genetically engineered bacterium and the fermentation production method disclosed by the invention, the total content of the sophorolipid is 358.77 g / L, and the lactone content accounts for 74% or more of the total sophorolipid, so that the separation and purification difficulty and the production cost are greatly reduced, and an efficient and low-cost solution is provided for industrial fermentation production of the sophorolipid.

The invention relates to the technical field of microorganisms, and discloses a candida tropicalis strain. The candida tropicalis strain is a candida tropicalis SDU2 strain, the classification name is Candida tropicalis, the preservation number is CGMCC No.34306, the preservation date is April 23, 2025, the preservation unit is China General Microbiological Culture Collection Center, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, Chaoyang District, Beijing. The candida tropicalis SDU2 strain has grease production capacity and high-temperature tolerance, and can convert waste biomass into biodiesel. The microbial inoculum prepared from the candida tropicalis SDU2 strain can be synchronously saccharified and fermented with biomass, so that the problem of high cost caused by poor strain adaptability in the traditional biomass high-valued comprehensive utilization process can be solved. The invention further discloses a candida tropicalis agent as well as a preparation method and application thereof.

The application belongs to the technical field of microorganisms, and provides application of Propionibacterium freudenreichii subsp. shermanii in preparation of products for improving constipation and depression, wherein the Propionibacterium freudenreichii subsp. shermanii NHNK-616 is preserved in the China Center for Type Culture Collection on July 1, 2024, and the preservation number is CCTCC NO: M 20241429. The inventors find that NHNK-616 has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolically producing gamma-aminobutyric acid, inhibiting growth of Candida albicans, inhibiting hypha formation of Candida albicans, combining with hypha of Candida albicans, and up-regulating expression of intestinal defensin and mucin genes, and therefore can be used for preparing products for improving constipation and depression.

The invention discloses a biological pretreatment method for improving the true protein production efficiency of wet distiller's grains, and belongs to the technical field of biological pretreatment, the method comprises the following steps: by combining chemical and biological multi-stage pretreatment modes, alkali and urea are combined in the first-stage chemical treatment process, and the second-stage chemical treatment process is combined in the second-stage chemical treatment process; the fiber structure of the wet distiller's grains is better damaged at normal temperature, and infectious microbe pollution is reduced for subsequent fermentation. In the second-stage anaerobic fermentation treatment, fibers in the wet distillers' grains are converted into sugar through the synergistic effect of bacteria and enzymes, the pH of the wet distillers' grains is reduced, and conditions are provided for growth of subsequent aerobic fermentation candida utilis yeast thalli. In the third stage of aerobic fermentation treatment of the candida utilis, urea and fiber in the pretreatment process in the early stage are used for degrading sugar to generate mycoprotein, so that the true protein improvement rate and the fiber degradation rate of the white spirit vinasse after pretreatment are effectively improved, the loss rate in the pretreatment process is greatly reduced, and finally, the true protein production efficiency of the wet white spirit vinasse is improved.

This invention discloses an overexpression Met15 This invention relates to a genetically engineered strain of *Candida utilis*, its construction method, and its applications, belonging to the field of bioengineering technology. The starting strain of the genetically engineered strain described in this invention is *Candida utilis*. C. utilis CCTCC NO: M 209298, strain overexpressing O-acetylhomoserine thioylase. Met15 Gene. This invention utilizes gene recombination technology to overexpress the O-acetylhomoserine sulfhydrylase encoding gene in *Candida utilis* (…). Met15 This can increase the content of L-methionine and L-cysteine ​​in yeast cells, increase the supply of sulfur-containing amino acid precursors, and ultimately increase the yield of SAM and GSH co-production in yeast cells.

The application provides a Candida viswanathii CATH2402, the preservation number of which is CCTCC NO: M 20241166, and also provides application of the aforementioned Candida viswanathii in fermentative production of azelaic acid, and a production method of azelaic acid. The Candida viswanathii CATH2402 can utilize various substrates to fermentatively produce azelaic acid, and has high tolerance to the product azelaic acid, thereby significantly improving the yield of azelaic acid by biological method, and having the potential for industrialized scale production.

The application discloses a biological pretreatment method for improving the efficiency of producing true protein from wet distiller's grains, and belongs to the technical field of biological pretreatment, which comprises the following steps: by combining chemical and biological multi-stage pretreatment methods, alkali and urea are used in the first chemical treatment process to realize better destruction of the fiber structure of the wet distiller's grains at normal temperature and reduce the contamination of miscellaneous bacteria in subsequent fermentation; the second anaerobic fermentation treatment converts the fibers in the wet distiller's grains into sugar through the synergistic effect of bacteria and enzymes, and reduces the pH of the wet distiller's grains, thereby providing conditions for the growth of Candida utilis in subsequent aerobic fermentation; and the third aerobic fermentation treatment utilizes the urea and fiber degradation sugar in the previous pretreatment process to generate cell protein, effectively improves the true protein improvement rate and fiber degradation rate of the distiller's grains after the pretreatment is completed, greatly reduces the loss rate in the pretreatment process, and finally improves the efficiency of producing true protein from the wet distiller's grains.

The application belongs to the technical field of microorganisms, and discloses a propionic acid-producing propionic acid bacteria NHNK-621 and application of the propionic acid bacteria in preparation of products for improving constipation and memory, the bacteria strain has been preserved in the China Center for Type Culture Collection on November 25, 2024, and the number is CCTCC NO: M 20242631. The propionic acid bacteria NHNK-621 provided by the application has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolically producing gamma-aminobutyric acid, inhibiting growth of Candida albicans, inhibiting hypha formation of Candida albicans, combining with hypha of Candida albicans, up-regulating intestinal defensin and mucin gene expression, in addition, the bacterial biofilm can increase the colonization ability to intestinal epithelial cells, and can be used for preparing products for improving constipation and memory.

The invention discloses a fermentation process and application of citric acid waste residues, and belongs to the technical field of biotech, the fermentation process comprises the following steps: S1, uniformly mixing citric acid waste residue powder, wheat bran and rice hull powder balls, performing high-pressure steam sterilization, adding sterile water, a urea solution and a basic nutrient solution, and uniformly stirring to obtain a fermentation substrate; s2, the fermentation substrate is placed in a fermentation tank, aspergillus niger liquid is inoculated, fermentation culture is conducted for 60-96 h, then candida utilis yeast liquid is inoculated, fermentation culture is conducted for 36-72 h, finally, lactobacillus plantarum liquid is inoculated, fermentation culture is conducted for 48-72 h, and the fermentation process is completed. Time sequence regulation and control are performed on flora metabolism, and finally the goals that the crude protein content of a fermentation product is obviously increased and the crude fiber content is effectively reduced are achieved.

The invention discloses application of leuconostoc mesenteroides subsp. Mesenteroides in improvement of constipation and depression, and belongs to the technical field of marine microorganisms. The invention discloses a leuconostoc mesenteroides subsp. Mesenteroides NHNK-613, which is preserved in the China Center for Type Culture Collection on July 1, 2024, and the preservation number of the leuconostoc mesenteroides subsp. Mesenteroides NHNK-613 is CCTCC (China Center for Type Culture Collection) NO: M 20241431. The NHNK-613 has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolizing to generate gamma-aminobutyric acid, inhibiting the growth of candida albicans, inhibiting the formation of candida albicans filaments, combining the candida albicans filaments and up-regulating intestinal defensin and mucoprotein gene expression; the traditional Chinese medicine composition can be used for preparing products for improving constipation and depression.

The application discloses a Candida utilis strain for efficiently converting ethanol / acetic acid and application thereof, and belongs to the technical field of single-cell protein production. In view of the technical problems of limited biomass accumulation, low carbon source conversion efficiency and low protein yield of a wild-type Candida utilis strain when the mixed carbon source of ethanol and acetic acid is used, the application provides the Candida utilis TU546 strain with the preservation number of CGMCC NO. 39030, and the strain is cultured in a fermentation medium containing the mixed carbon source of ethanol and acetic acid, so that the fermentation strain body with high protein content is obtained, and efficient conversion from the mixed carbon source of ethanol and acetic acid to single-cell protein is realized. The strain and the method thereof are mainly used for producing single-cell protein products, and can be used as feed or food additives, and have important application value in the microbial fermentation industry.

The invention discloses a fermented feed additive for replacing soybean meal as well as a preparation method and application of the fermented feed additive. The fermented feed additive disclosed by the invention is prepared by performing acid protease enzymolysis on a mixed fermentation raw material and fermenting the mixed fermentation raw material with a complex microbial inoculant, wherein the mixed fermentation raw materials comprise the following components in parts by weight: 40-80 parts of corn protein powder, 30-60 parts of corn steep liquor, 7-12 parts of corn germ meal, 4-15 parts of molasses and 2-5 parts of extruded soybeans; the complex microbial inoculant is composed of pediococcus acidilactici, candida utilis and bacillus subtilis according to the viable count ratio of (1-3): (1-3): (0.1-1). The fermented feed additive can effectively replace soybean meal in animal daily ration, the utilization rate of nutrients by animals is increased, the health level of the animals is improved, and the production performance of the animals is improved.

The present invention relates to: an antisense oligonucleotide (ASO) targeting an FKS1 gene, an FKS2 gene, a CHS3 gene and / or a GWT1 gene of a Candida sp. fungus; an antibacterial composition comprising same; and a pharmaceutical composition for preventing or treating diseases caused by pathogenic fungi. The ASO according to the present invention can be used as a gene therapeutic agent having a fungus-specific antibacterial effect by specifically inhibiting FKS1, FKS2, CHS3 and / or GWT1 genes, and can be a useful gene therapeutic agent that can be used in combination with or replace conventional antifungal agents for antifungal agent-resistant fungi or multidrug-resistant fungi.

This application discloses an acid-tolerant Cyberlindnera jadinii yeast strain and its applications, belonging to the field of microbial technology. The Cyberlindnera jadinii yeast strain is Cyberlindnera jadinii C-12-2, which was deposited on Mar. 5, 2024, at the Guangdong Microbial Culture Collection Center, with the deposit number GDMCC NO: 64388. The Cyberlindnera jadinii yeast disclosed in this application can grow in a medium with a pH of 3.5, and its crude protein content can reach 70.02% after 24 hours of fermentation. The yeast strain disclosed in this application was obtained through heavy-ion irradiation mutagenesis followed by acid adaptation. It has been determined that the strain can grow in an acidic medium with a pH of 3.5, overcoming the growth limitation of the original strain at low pH values.

The invention discloses a candida tropicalis engineering bacterium for producing 1, 2, 4-butantriol as well as a construction method and application of the candida tropicalis engineering bacterium, and belongs to the technical field of microorganisms. According to the recombinant candida tropicalis strain constructed by the invention, scaffold protein ligand-enzyme fusion protein is subjected to integrated expression through metabolic engineering transformation, and a scaffold protein structural domain GBD-SH3-PDZ is overexpressed on the basis of the scaffold protein ligand-enzyme fusion protein; a glucose repression gene MIG1 gene is knocked out; the KdcA mutant is subjected to overexpression, the yield of 1, 2, 4-butantriol can be obviously increased, the shake flask yield of 1, 2, 4-butantriol is increased to 2.1 g / L from 0.6 g / L of the original strain BTO-01, the yield is increased by 3.35 times, and the yield of the strain BTO-14 in a 5L tank reaches 11.18 g / L through amplification culture of a fermentation tank.

The application belongs to the technical field of cosmetics, and discloses an avocado fermentation composition and a preparation method and application thereof. The composition comprises 1%-10% of avocado fermentation filtrate, 0.1%-2% of alpha-glucan oligosaccharide, 0.1%-2% of wild chrysanthemum extract, 3%-8% of a humectant, 0.01%-0.1% of a chelating agent, and the rest of deionized water. The avocado fermentation filtrate is obtained by complex fermentation of aphid pseudomycetes and candida fungi, and is rich in oligopeptides, free amino acids, lactic acid and polyphenols. The application scientifically compounds the filtrate obtained by fermenting avocados with specific complex strains and prebiotics with clear bacterial flora selectivity, and constructs a multifunctional composition with clear structure, synergistic function and high stability. The composition not only provides direct barrier repair and moisturizing effect, but also actively guides the skin micro-ecosystem to healthy succession through nutritional intervention, thereby synchronously improving the skin health level in two dimensions of molecules and ecology.

The application belongs to the field of environmental protection technology of biological repair of wastewater, and discloses a kind of immobilized adsorption microspheres, which comprise sea kobe bacteria, Candida guilliermondii and European nitrosomonas bacteria, and are prepared by immobilized embedding technology. The microspheres of the application can effectively repair glutamic acid high ammonia nitrogen fermentation wastewater.

The application relates to the cosmetic technical field, and particularly discloses a Camellia nitidissima seed fermentation oil with multiple effects and a preparation method and application thereof. The preparation method of the Camellia nitidissima seed fermentation oil comprises the following steps: solid-state fermentation is carried out on Camellia nitidissima leaf blades by using Camellia nitidissima spore liquid, and the fermented Camellia nitidissima leaf blades are crushed into Camellia nitidissima leaf powder; the Camellia nitidissima seed oil and the Camellia nitidissima spore liquid are inoculated in a liquid culture medium, and a product is obtained by jointly carrying out liquid fermentation on the Camellia nitidissima spore liquid, Candida sp. and Lactobacillus plantarum bacterial liquid and the Camellia nitidissima leaf powder; the product is subjected to high-speed centrifugation, the supernatant oil phase is taken, and sterilization is carried out through a microporous filter membrane to obtain the Camellia nitidissima seed fermentation oil. The Camellia nitidissima seed fermentation oil has a fragrant smell, is not prone to deterioration and oxidation, has a better shelf life and stability, and has a better repair and anti-aging effect.

The application relates to the field of building waterproofing, and discloses a composition for a permeable crystalline waterproof coating, the permeable crystalline waterproof coating, and a preparation method and application thereof. The composition contains a main agent and an auxiliary agent; the main agent comprises cement, bioactive microspheres, a high-molecular water-absorbing polymer, a filler and latex powder; the cement is a combination of aluminate cement and portland cement with a content mass ratio of 1:11-55; the bioactive microspheres are prepared by the following method: mixing mixed bacterial liquid containing bacillus pasteurii and candida utilis with sodium alginate and calcium acetate to obtain the bioactive microspheres. The permeable crystalline waterproof coating provided by the application has excellent impermeability, bonding performance, repair performance and durability.

The application discloses an organic fertilizer suitable for tea yield increase and insect killing and a preparation method thereof, and relates to the technical field of organic fertilizer and microbial fertilizer manufacturing. The organic fertilizer comprises straw, chicken manure, Tibetan wood incense, worry grass, mouse grass, black bone vine, tannic acid-rice protein enzymatic hydrolysate, sodium laurate modified diatomite, sodium humate, urea, compound microbial bacteria and water. The compound microbial bacteria are composed of Candida utilis and intercalary neura. The compound microbial bacteria in the organic fertilizer are helpful to accelerating the fermentation and decomposition of the straw, chicken manure, sodium humate, urea and traditional Chinese medicine plants, promoting the conversion of macromolecular substances into small-molecule active ingredients, and then assisting with the tannic acid-rice protein enzymatic hydrolysate and sodium laurate modified diatomite, so that the yield of tea tree planting is improved through reasonable proportioning of nutritional ingredients.

The invention discloses application of a Candida tropicalis strain (Candida tropicalis) to degradation of TNT (trinitrotoluene), and further provides a screening method of the Candida tropicalis strain (Candida tropicalis). The invention also provides an application of the TNT efficient degradation strain. According to the present invention, the Candida tropicalis strain can tolerate more than 400 ppm of TNT, can complete the efficient degradation of TNT within 10 h, can effectively solve the problems of low tolerance concentration, slow metabolism rate and the like of the existing TNT metabolism microorganisms, and is mainly used for solid TNT microbial conversion, TNT wastewater treatment and in-situ purification of TNT contaminated soil.

This invention belongs to the field of microbial technology, specifically a high-protein-producing *Candida utilis* strain and its applications. *Candida utilis* (… Cyberlindnera jadinii )24-3, deposited on November 27, 2025 at the Guangdong Provincial Microbial Culture Collection Center, accession number GDMCC No:67370. This high-protein Candida utilis produces single-cell protein. This invention relates to the production of single-cell protein by Candida utilis. C. jadinii 2.615 strain was mutagenized to obtain the mutant strain Candida utilis 24-3. The mutant strain was then used to rapidly and cost-effectively produce single-cell protein by using bacterial bran hydrolysate or the product of industrial waste gas treatment as substrates, thereby reducing the treatment costs of the two types of waste.

The invention discloses a fermented feed for fattening pigs, which is prepared by taking dry white vinasse, rapeseed meal, fresh forage grass, grain starch and a premix for fattening pigs as raw materials, inoculating bacillus subtilis liquid, candida utilis liquid, lactobacillus plantarum liquid and lactococcus lactis liquid, and fermenting for 72 hours. Experimental results show that compared with a mixture before fermentation, the fermented vinasse mixed feed has the advantages that the crude protein improvement rate can reach 16-22%, the pH value is 3.8-4.4, and the fermented vinasse mixed feed is sour and fragrant in taste, good in palatability and rich in compound probiotics and functional ingredients. The fermented feed solves the problems of poor palatability and unbalanced nutrition of the Maotai wine vinasse, rapeseed meal and pasture which are directly fed, realizes high-value utilization of the low-value feed raw materials, can improve the utilization rate of the feed raw materials, can inhibit the reproduction of harmful bacteria in animal intestinal tracts, can promote the health of the animal intestinal tracts, and can improve the animal immunity; if fattening pigs are fed for a long time, the feed cost can be greatly reduced and the pork quality can be improved.