219 results about "Candida sp" patented technology

The invention provides a high-efficiency compound microbial inoculum for fermenting beds, particularly relates to a compound microbial inoculum for the fermenting beds, and a preparation method and application thereof. The compound microbial inoculum is composed of five beneficial bacterium strains (Bacillus subtilis CGMCC1.769 and CGMCC1.210, lactobacillus plantarum CICC6073, Candida tropicalis CGMCC2.587 and Trichoderma koningii CGMCC3.4290) and metabolites thereof (amylase, protease, lipase, cellulase, organic acid, bacteriophage and the like). The compound microbial inoculum is prepared by using a submerged liquid mixed fermentation technique. The method comprises the following steps: carrying out liquid submerged fermentation on the lactobacillus plantarum at 35 DEG C for 12 hours, respectively inoculating Candida tropicalis and Trichoderma koningii, carrying out mixed fermentation at 30 DEG C for 12 hours, inoculating Bacillus subtilis, carrying out liquid submerged mixed fermentation for 24 hours, respectively controlling the fermented viable count at 1-2*10<9> cfu / mL, 5-8*10<8> cfu / mL, 3-5*10<8> cfu / mL, 3-5*10<9> cfu / mL and 2-5*10<9> cfu / mL, finishing fermentation, packaging and sealing to obtain the high-efficiency compound microbial inoculum for the fermenting beds. The invention also provides application of the high-efficiency compound microbial inoculum-a preparation method of padding material. The compound microbial inoculum has the advantages of high viable count, high biological activity and low impurity bacterium pollution; the compound microbial inoculum can obviously reduce the odor of pigsties and reduce the living possibility of mosquitoes and flies, thereby achieving the ecological culture goals of no pollution and zero discharge; and the process for manufacturing the ferment bed is simple, thereby saving the padding material, human power and the like.

The invention provides a method for preparing a novel ruminant feed by using beneficial bacteria and plant fiber. The method comprises the following steps of: mixing various fermentation substrate raw materials in a blending mixer, sterilizing, cooling, and inoculating first mixed bacterium liquid for constant-temperature fermentation for 32-48h; and then inoculating second mixed bacterium liquid for secondary fermentation for 48-72h according to the same operation steps; carrying out the low-temperature drying on the fermented materials, and smashing to obtain a finished product, wherein the fermentation substrate comprises 8-11 parts by weight of rice husk bran, 8-11 parts by weight of sunflower husk power, 8-11 parts by weight of corn peel, 8-11 parts by weight of bran, 8-11 parts by weight of distilled spirit vinasse, 8-11 parts by weight of potato pulp, 2-5 parts by weight of apple pomace, 8-11 parts by weight of tomato pomace, 8-11 parts by weight of corn stalk powder, 8-11 parts by weight of corn glycoprotein, 2-5 parts by weight of bean dregs and 1-3 parts by weight of urea, the first mixed bacterium liquid is a mixture of trichoderma koningii and green trichoderma liquid, and the second mixed bacterium liquid is a mixture of feed bacillus and candida utilis bacteria liquid.

The invention relates to a long-chain dibasic acid producing strain and a preparation method and application thereof, and solves the problems of non-significant improvement effect of a long-chain dibasic acid strain in the prior art; the classification name is Candida (Candida sp.)TDTC016, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.8928. The long-chain dibasic acid producing strain can be widely used in the field of preparation of a long chain dibasic acid.

A preparation technique of chitosan microspheres immobilization lipase comprises the steps that: (1) lipase from candida SP is dissolved in phosphate buffer solution by taking chitosan microspheres as an immobilization carrier and adopting glutaraldehyde cross-linking, lipase A is obtained. (2) candida SP lipase solution of 0.70-0.80mg / mL prepared by phosphate buffer solution b with a pH value of 6.5-7.5 is added with immobilization lipase A (final concentration is 0.005-0.0055g / ml) and after being stirred, ethyl (3-dimethylamino propyl) carbodiimide hydrochloride solution is added, the mixture is then stirred to be fully immobilized, chitosan microspheres immobilization lipase B is obtained after the treatment of the mixture. The chitosan microspheres immobilization lipase prepared by the preparation technology of the invention improves apoenzyme combination rate and activity recovery, has powerful immobilization lipase activity and specific activity and the immobilization lipase can be recycled for six times.

A bioprocess for producing carboxylic acids, alcohols and aldehydes is provided by culturing Candida sp. in a fermentation medium containing various defined substrates.

The invention relates to a preparation method and application of a compound leavening agent. The preparation method comprises the steps of: separating lactobacillus plantarum, lactobacillus bulgaricus, streptococcus thermophilus, saccharomyces cerevisiae, candida rugosa, candida tropicalis and fermented pichia pastoris, as a leavening agent, from naturally fermented panada to produce a strain so as to prepare freeze-dried strain powder; weighing 1-2 parts of lactobacillus plantarum, 1-2 parts of lactobacillus bulgaricus, 1-2 parts of streptococcus thermophilus, 3-5 parts of saccharomyces cerevisiae, 3-5 parts of candida rugosa, 3-5 parts of candida tropicalis and 3-5 parts of fermented pichia pastoris based on parts by weight, and uniformly mixing to obtain the compound leavening agent. When the compound leavening agent is applied to the cooked wheaten food, bacterial contamination can be effectively prevented, the sanitation of the cooked wheaten food is guaranteed, the quality of the cooked wheaten food is improved, the production cycle is shortened, a production process is controllable, and the preparation method of the compound leavening agent lays a foundation for industrial production.

The invention provides a compound bacterial agent for purifying a black and odorous water body and a preparation method thereof. The compound bacterial agent comprises the following components: actinomycetes, bacillus subtilis, nitrifying bacteria, lactic acid bacteria, candida utilis and pseudomonas aeruginosa in a weight ratio of 2:2:2:1:1:1. The invention has the following advantages: the COD content, ammonia nitrogen content and phosphate salt in the black and odorous water body can be effectively reduced, and the odor of the black and odorous water body can be eliminated; and meanwhile, by adding the ammonia nitrogen removal strain, the transparency of the water body can be gradually improved.

The invention relates to a production process for bioorganic fertilizer with high bacteria content. The production process comprises the following steps: separately activating a trichoderma viride strain and a trichoderma pseudokoningii strain; separately activating a bacillus amyloliquefaciens strain, a bacillus subtilis strain and a candida utilis strain; carrying out step-by-step enlarged culture and the like. A preparation method provided by the invention has the advantages of low cost, simple process, high operability and convenience in popularization and application; the obtained bioorganic fertilizer is high in content of effective living bacteria; a large amount of corn stalk resources can be really and efficiently converted, soil is remediated, the fertility of the soil is improved, and wastes are changed into valuables; in addition, the problem of environmental pollution caused by straw burning can be relived to a certain degree.

The invention relates to the technical field of biology, and relates to a lipase mutant and uses thereof. The mutant is obtained from wild type candida antarctica lipase B (CALB) through mutation. The invention particularly relates to the lipase mutant, a preparing method thereof, and a method of preparing S-configuration ibuprofen type compounds with optical activity through catalysis of hydrolysis of ester derivatives of the ibuprofen type compounds by utilization of the lipase mutant and through kinetic resolution. The wild type candida antarctica lipase B is subjected to mutation to obtain the modified lipase. The lipase is expressed in pichia pastoris engineering bacteria. The lipase comprises an amino acid sequence having at least 85% of identity with the SEQ ID NO.02. The 189 site residue corresponding to the SEQ ID NO.02 of the lipase is an aromatic amino acid residue and the 190 site residue corresponding to the SEQ ID NO.02 of the lipase is a nonpolar amino acid residue. By subjecting the wild type candida antarctica lipase B to mutation, the enantioselectivity of the lipase to the ibuprofen type compounds is improved.

The invention discloses a candida tropicalis strain CAT H1614, a microbial agent comprising the same and an application thereof, and a method for producing long chain diprotic acid by fermentation anda preparation method of the long chain diprotic acid. The candida tropicalis strain CAT H1614 of the invention can maintain high vitality for a long time in an environment with a pH lower than 7.0, thereby being particularly suitable for production of the long chain diprotic acid by a biological method, acid production is rapid during fermentation, the yield is high, and a fermentation cycle canalso be reduced. The fermentation method and the preparation method of the long chain diprotic acid provided by the invention have significant cost advantages, can effectively alleviate pressure on resources and environment, and thus have very obvious industrialization value advantages.

The invention discloses a zymophyte liquid, a liquid spirit stillage fermented feed and a fermentation method of the liquid spirit stillage fermented feed. The zymophyte liquid is prepared by mixing, at a preset proportion, liquids of lactobacillus, bacillus subtilis, bacillus natto and candida utilis bacteria which are obtained by enlarged culture. The fermented feed is prepared by fermenting a liquid complete feed with the zymophyte liquid. The fermentation method comprises the steps of inoculating the zymophyte liquid with the liquid complete feed at 3-5%wt, adjusting the moisture content to be 68-72%, sufficiently mixing into a liquid, and loading into a sealer for sealing and fermentation. The shelf life of the liquid spirit stillage fermented feed fermented by the zymophyte liquid is over 6 months at a room temperature. The liquid spirit stillage fermented feed is easier to digest, low in thallus activity loss and ideal in using effect, a culture proportion and a complete feed formula balance essential amino acid, and the liquid spirit stillage fermented feed is suitable for a whole postweaning growth stage of a commercial pig.

The invention discloses a preparation method of semi-aromatic polyamide based on furan dicarboxylic acid. The semi-aromatic polyamide based on furan dicarboxylic acid is prepared by using dimethyl furandicarboxylate and aliphatic diamine as comonomer and using Candida sp.99-125 lipase as a catalyst to perform polymerization reaction in a solvent. Compared with the prior art, the preparation method provided by the invention has the advantages of simple process, mild reaction conditions, total yield up to 60-80% and molecular weight up to 54000 g / mol. Compared with PPA, the semi-aromatic polyamide based on furan dicarboxylic acid, provided by the invention, shows similar glass transition temperature and crystalline structure, and has lower melting point and better processability.

The invention provides a preparation method of a soil ecological restoration agent for solving old ginseng land continuous cropping obstacle. The preparation method includes the steps: (1) mixing 20-40 parts of bacillus licheniformis, 10-20 parts of candida utilis, 10-30 parts of lactobacillus plantarum, 10-20 parts of trichoderma viride and 5-10 parts of rhodopseudomonas palustris according to the weight ratio to obtain compound bacteria; (2) adding the compound bacteria to a compound culture medium according to the weight ratio of 1:(80-120), stirring evenly, then culturing under a condition of the temperature of 28-32 DEG C until the pH is less than 4.00, then carrying out closed culture for 12-15 days, and thus obtaining the soil ecological restoration agent in a liquid form; and furthermore, making the liquid form into a solid form. Through the production of the microbial inoculant, starting from restoration of a soil ecological system, and with use of the multi-strain combined technology method, beneficial bacteria in soil and beneficial nutritional factors for promoting the growth of ginseng are increased, and harmful bacteria generated by continuous cropping are inhibited.

The invention relates to a method for preparing rare sugar alcohol, which belongs to the field of biotechnology. The invention describes a method for preparing a xylitol and L-arabinose mixed crystal from xylose mother liquid by a biological method. The method comprises the following steps of: inoculating yeast (Candida sp)X828 CGMCC No.3691 which cannot use L-arabinose as a unique carbon source to a xylose mother liquid culture medium to ferment; consuming glucose and galactose in the xylose mother liquid; reducing the xylose to obtain xylitol so as to obtain xylitol and L-arabinose-containing fermentation liquid; and concentrating, desalting, reconcentrating and crystallizing the fermentation liquid to obtain the xylitol and L-arabinose mixed crystal. In the method, an additional value of the xylose mother liquid can be increased; and the xylitol and L-arabinose mixed crystal can be directly used for preparing a product which comprises the L-arabinose and the xylitol at the same time.

The invention provides a method for producing biologic protein feed by using solid-state fermentation fiber dreg. The method comprises the steps: activating, inoculating, cultivating and fermenting lignin trichodermin strain, candida utilis and saccharomyces cerevisiae to obtain a first liquid strain and a second liquid strain; adding maize flour, ammonium sulfate and potassium dihydrogen phosphate in maize soaking water, regulating pH value, conducting steam sterilization to obtain liquid nutrient solution; evenly agitating the dried wet maize peel dreg with bran, maize steep liquor, bean pulp, rice chaff and urea together, regulating pH value to obtain starting crude base stock; evenly mixing the liquid nutrient solution and the starting crude base stock, then inoculating, fermenting, continuously agitating and supplying fresh air, simultaneously adding inorganic salt, and evenly mixing; and drying the fermented materials at the temperature lower than 55 DEG C to obtain the biologicprotein feed. According to the method, the biologic protein feed is produced by utilizing wet maize peel dreg generated in process of producing starch from maize through wet method, and maize soakingwater, so that the additional value is increased, and the cost is lowered.

The invention aims at providing a compound microecological preparation for livestock and poultry excrement treatment and a preparation method and application thereof. For 100 g of livestock and poultry excrement, the compound microecological preparation needed to be added is prepared from, by volume, lactobacillus casei 0.03-0.10 Ml, enterococcus faecalis 0.03-0.10 mL, candida mycoderma bacteria 0.05-0.50 mL and bacillus subtilis 0.03-0.10 mL, wherein effective living bacterium number of bacterium liquid of the lactobacillus casei, the enterococcus faecalis, the candida mycoderma bacteria and the bacillus subtilis is 1.0 * 109 cfu / mL. The compound microecological preparation is utilized for fermentation of livestock and poultry excrement, the pH and the enterococcus faecalis number of the preparation can be reduced (P> 0.05), the content of indole is reduced by 58% (P< 0.05), and the compound microecological preparation has the great significance on fecal odor elimination.

The invention provides a production process of fermented cottonseed meal. The process comprises the following steps: breeding lactobacilli, bifidobacteria, bacillus subtilis and candida utilis by adopting a self-made culture medium at the same time, mixing obtained strain liquid, acid protease, cellulase, aspergillus oryzae and yeast enzyme with cottonseed meal, performing fermentation production, drying, grinding and packaging. According to the fermented cottonseed meal produced by adopting the process, free gossypol in the cottonseed meal can be effectively degraded, the palatability of the cottonseed meal can be improved, macromolecular proteins are decomposed into micromolecular polypeptides or amino acids convenient to absorb by animals, crude proteins and crude fibers are degraded, a large quantity of active probiotics are secreted and synthesized, and the nutritional value of the cottonseed meal as a feed is improved.

The invention relates to a probiotic feed additive for Chinese softshell turtles and a preparation method of the probiotic feed additive. The probiotic feed additive is prepared from probiotics and carrier fillers, wherein the carrier fillers are sterilized wheat bran, and the probiotics include bacillus subtilis, bacillus natto, Lactobacillus casei and candida utilis with the ratio of (0.4-0.6):(0.4-0.6):(0.8-1.2):(0.8-1.2), and the probiotics are added in the aseptic carrier fillers to prepare probiotic powder. The probiotic feed additive can improve the growth performance of the Chinese softshell turtles, reduce the food coefficient, improve the immunological function and reduce the diseases, has the advantages of low cost, no pollution, no drug resistance, no residue and the like, and is environmentally friendly.

The invention relates to low-yield isoamylol and high-yield beta-phenylethanol yeast, an isolated culture method thereof and an application of the yeast. The low-yield isoamylol and high-yield beta-phenylethanol yeast is named as a Candida humilis YS023A strain, the strain is collected in a general microorganism center of a China Committee for Culture Collection of Microorganisms on No. 3, No. 1 courtyard Beichen West Road, Chaoyang District, Beijing on February 25, 2019, and the collection number of the strain is CGMCC NO:17257. The yeast has excellent characteristics of low-yield isoamylol and high-yield beta-phenylethanol and excellent sugar, alcohol and acid resistance, and the sugar utilization rate, the fermentation capacity and the ethanol production capacity of the yeast are good.The yeast is used for production of strong-flavor and rice-flavor Baijiu and has great significance for increasing of high-quality product rate and wine outlet rate of raw wine.

The invention relates to a biological protein feed and a production method thereof. The production method comprises: crushing corn straw into particles capable of being screened with a 20 mesh sieve, and adopting a rail type autoclave to carry out a moisture and heat steam treatment for 30 min at a temperature of 100 DEG C; adopting a two-step method to ferment, wherein Gloeophyllum trabeum is firstly inoculated to ferment, urea, magnesium sulfate and potassium dihydrogen phosphate are added to the obtained primary fermentation product, uniform mixing is performed, and Aspergillus niger, Aspergillus oryzae, Bacillus subtilis and Candida utilis are inoculated to carry out secondary fermentation; drying after completing the fermentation; and carrying out 40 mesh crushing to obtain the biological protein feed. According to the present invention, the biological protein feed production method has characteristics of simple process and low cost; with application of the produced biological protein feed to feed milk cows, the milk yield can be increased by more than 4.7%, and the milk protein content can be increased by more than 4.1%; and the produced biological protein feed is suitable for various types of high, medium and low-end milk cow farming customers, and can further be used for feeding other livestock and poultry including piglets, chickens, ducks, geese and other poultry.

The invention relates to an inoculating culture and detecting method for gathering copper ions through saccharomycete. According to the inoculating culture method, at first, inoculating culture media with different copper ion concentrations and a basal culture medium are prepared; the inoculating culture media with the different copper ion concentrations are inoculated with a cultured candida utilis strain solution, and shaking culture is carried out; candida utilis with a high OD600 value and the low content of the copper ions in supernatant liquor is selected from the cultured strain solution to serve as starting strains; a starting strain solution is taken out, the inoculating culture medium with the smallest copper ion concentration is inoculated with the starting strain solution, after shake-flask culture is carried out, the strain solution is absorbed again, the inoculating culture medium with the second smallest concentration is inoculated with the strain solution, and inoculating culture is carried out according to the method; finally, copper ion conversion rates of different generations of the candida utilis are detected, and when the conversion rates of the copper ions are unchanged or reduced, the strains are strains for producing copper gathering yeast. The cultured saccharomycete can grow in the culture medium with the large copper ion concentration, the conversion efficiency of the copper ions is high, and the basis is provided for development of the new function of the yeast.

The invention relates to the field of biological feeds, in particular to a preparation method of a biological feed, which comprises the following steps: the mixture of pine needles with rich nutrientsand strong adsorbability, radix isatidis, hypericum perforatum, star anise leaf, bean dregs, albumen powder, wheat bran and other cake dregs is used as the culture medium; candida utili can keep themicroecological balance of the intestines and inhibit harmful bacteria from breeding; natto and secretions thereof can promote the synthesis of the prolease so as to promote the decomposition of the proteins in the feed and increase the utilization rate of the proteins in the feed; Chinese herbs are used for promoting growth by utilizing the functions of killing, antibiosis, health care and no drug tolerance on the pathogenic microorganisms; and the microorganisms are utilized to regulate the biological balance of stomachs and intestines of animals so as to promote the growth and the breedingof the beneficial organisms. On one hand, the beneficial microbial inoculums can inhibit the causal organisms stuck to the alimentary tract and neutralize the toxic substances; and on the other hand,the beneficial microbial inoculums can prevent the absorption of toxin and waste, reduce the production of ammonia in the alimentary tract, reduce the odious smell of the manures, reduce the residuesof heavy metals in the product and fundamentally cut off the disastrous harm to the cultivation brought about by the long-term application of the antibiotics so as to ensure the health of the animals.

The invention discloses a production method of a large-scale rice wheat straw microorganism feed, and belongs to the field of biological feeds. Rice wheat straw is used as a main carbon source, and inorganic nitrogen is used as a main nitrogen source. The production method comprises the following steps of performing high-efficient pure solid cultivation on a white rot fungus enzyme preparation, then converting the cultivated white rot fungus enzyme preparation in large inoculum amount to a first-stage fermentation medium of the rice wheat straw, and besides, inoculating serial lignocelluloses degradation bacteria and candida utilis bacteria for synergistic rapid degradation and transformation; adding bacillus and saccharomyces cerevisiae for second-stage fermentation; and using lactic acid bacteria for third-stage anaerobic fermentation so as to obtain finished products. The lignin of the straw biological feed is greatly reduced, genuine protein is increased to 11%b or above, and the raw materials do not need to be sterilized; the finished products do not need drying treatment, lysozymes are enriched, and the production cost is extremely low. The large-scale rice wheat straw microorganism feed can be used as a superior coarse feed and a protein grain feed for cattle and sheep, and the additive quantity can reach 60% or above; the additive quantity can also be about 10%, so that the large-scale rice wheat straw microorganism feed is used for simple stomach animals. The large-scale rice wheat straw microorganism feed is suitable for mass production, and the feeding and economic effects are obvious.

The invention relates to the technical field of feed additive and processing thereof, and in particular to a probiotic feed additive. The probiotic feed additive is prepared by mixing autonomously separated lactobacillus plantarum, candida rugosa, candida ethanolica and lactococcus garvieae and buffalo milk fermentation substrate to ferment. The preparation process disclosed by the invention is simple, and the probiotic feed additive can be widely applied in the raising of livestock and poultry such as milk buffalos, pigs, chickens, cattle and sheep. The probiotic feed additive can remarkably improve the intestinal microbial floras of animals and enhance immunity, and can reduce the usage of antibiotic medicines and eliminate medicine residue, and when applied in production, the probiotic feed additive can shorten feeding periods and save feeds, thus increasing farming income.

The invention relates to a method for preparing a novel biological microcapsule capable of being applied to treatment of ammonia nitrogen and soluble organic substances in water. According to the method for preparing the microcapsule, no chemical crosslinking agent or external material is added as a carrier, but candida is spontaneously combined to an extracellular polymer excreted by fungal hyphae cell aggregation in a natural mode under a special condition, and an appropriate condition is created to induce the intergrowth of hyphomycete and the candida so as to form the biological microcapsule in the shape of a solid microsphere. The novel biological microcapsule can efficiently degrade the ammonia nitrogen and the soluble organic substances in water, can keep the activity of thalli, separates the thalli from the outside poor environment, slows down the influence caused by temperature and pressure, and is favorable for uniform distribution in water, and also favorable for stability in storage and use. The preparation method is simple and feasible, has less influence factors, and has great potential and application value for treating the ammonia nitrogen and the soluble organic substances in the water.

The invention discloses a preparation method of a magnetic biological microcapsule for processing organic matters and ammonia nitrogen in water. The method comprises the following steps: 1, adding Candida yeast living cells into a disinfected YPD culture medium, culturing at 25-28DEG C under 150-200rpm for 5-6h, taking a bacterial strain liquid obtained after a logarithmic phase, carrying out low temperature drying refrigeration, centrifuging, washing, and carrying out suspension extraction by using normal saline to obtain a bacterial strain Candida yeast suspension with the OD value of 1.5-1.8; 2, mixing sodium alginate and Fe3O4 powder, adding the obtained mixture to deionized water according to the addition amount of sodium alginate of 19-21g / L and the addition amount of the Fe3O4 powder of 1.8-2.2g / L, heating until sodium alginate is completely dissolved, and cooling; and 3, uniformly mixing the yeast suspension with the obtained sodium alginate-iron powder solution according to a volume ratio of 1:9-1:11, adding the obtained solution mixture into a calcium chloride solution in a dropwise manner, curing for 30-40min, and filtering to obtain the microcapsule. The magnetic biological microcapsule prepared in the invention can efficiently degrade the organic matters and ammonia nitrogen in water, can maintain the vitality of thalli, separates the thalli from extraneous adverse environment, can be uniformly distributed in water and separated from water, and is stable in the storage and use process.

The invention relates to a novel method for extracting fulvic acids from industrially-produced waste edible fungi residues by fermentation. The novel method includes the steps of (1) preparing a primary seed solution; (2) preparing a secondary seed solution; (3) inoculating the secondary seed solution, bacillus subtilis, candida tropicalis and trichoderma pseudokoningii into a 1000-2000L of fermentation tank respectively and fermenting for 10-15 days so as to obtain a fermented seed solution; (4) treating pleurotus ostreatus residues; (5) inoculating the fermented seed solution into the pleurotus ostreatus residues to be inculated, and fermenting at the temperature 20-40 DEG C aerobically for 4-5 days so as to obtain fermented fungi residues; (6) extracting the fulvic acids; (7) performing spray drying so as to obtain flavescent fulvic acid powder. The fulvic acid content can be above 35% after fermentation, and yield of crude fulvic acids can be up to about 30% (dry matter percentage) through extraction.

The invention discloses a composite microbial agent for degrading kitchen waste and a preparation method thereof. The preparation method comprises the following steps: respectively inoculating bacillus subtilis, bacillus licheniformis and bacillus coagulans into a beef extract peptone culture medium for culturing; respectively inoculating candida utilis and plant lactobacillus into a potato glucose culture medium for culturing; and performing mixed fermentation on the bacillus subtilis, bacillus licheniformis, bacillus coagulans, candida utilis and plant lactobacillus to prepare the compositemicrobial agent. By researching compounding of multiple microbial agents, the synergistic effect among strains in a proper proportion is discovered, and main components (cellulose, grease, starch andprotein) of the kitchen waste can be effectively degraded, so that the kitchen waste can be fully fermented. Moreover, the preparation process provided by the invention is simple, and the production benefits are high.

The invention discloses a strain for producing 3-hydroxyl propionic acid at high yield, which is a compound mutant strain of Candida sp., is stable in hereditary features and yield, is applicable to industrial popularization, and is collected in the China general microbiological culture collection center with the collection number of CGMCC No.5344. The invention further discloses a method for converting propionic acid into 3-hydroxyl propionic acid. The method comprises the following steps of when the growth of the strain reaches a stable stage, supplementing glucose in a limited way till theconcentration of the strain is 15-25g / L when the growth of the strain reaches a stable stage; controlling the concentration OD600 of the strain is 18-20; adding propionic acid, potassium hydroxide, strong sodium oxide and ammonia water (according to a ratio of 8: 1: 1: 1) in a flowing way, so as to keep the concentration of the propionic acid at 10-20g / L; and fermenting and culturing for 96 hoursto obtain a fermentation liquid of 3-hydroxyl propionic acid. After optimization, the yield of 3-hydroxyl propionic acid on the culture medium is increased to 30g / L.