64 results about "Candida rugosa" patented technology

The invention relates to a preparation method and application of a compound leavening agent. The preparation method comprises the steps of: separating lactobacillus plantarum, lactobacillus bulgaricus, streptococcus thermophilus, saccharomyces cerevisiae, candida rugosa, candida tropicalis and fermented pichia pastoris, as a leavening agent, from naturally fermented panada to produce a strain so as to prepare freeze-dried strain powder; weighing 1-2 parts of lactobacillus plantarum, 1-2 parts of lactobacillus bulgaricus, 1-2 parts of streptococcus thermophilus, 3-5 parts of saccharomyces cerevisiae, 3-5 parts of candida rugosa, 3-5 parts of candida tropicalis and 3-5 parts of fermented pichia pastoris based on parts by weight, and uniformly mixing to obtain the compound leavening agent. When the compound leavening agent is applied to the cooked wheaten food, bacterial contamination can be effectively prevented, the sanitation of the cooked wheaten food is guaranteed, the quality of the cooked wheaten food is improved, the production cycle is shortened, a production process is controllable, and the preparation method of the compound leavening agent lays a foundation for industrial production.

The invention discloses compound lipase and application thereof. The compound lipase comprises Novozym435 lipase, candida rugosa lipase and wild DF15 lipase according to the mass ratio of (4-6):(5-8):(2-3). The Novozym435 lipase, the candida rugosa lipase and the wild DF15 lipase are mixed, so that the enzymolysis time is shortened, the enzymolysis efficiency and the enzymolysis degree are improved, and furthermore, the flavor components of zymolyte are enriched. Butter zymolyte prepared from the compound lipase is more mellow and richer in fragrance, the sour and greasy smell is proper, the milk fragrance and the fat fragrance are extremely harmonized, and the compound lipase has an obvious effect on milk type essences such as flavoring butter essences.

The invention discloses a universal strategy for efficiently improving enzyme thermodynamics stability. The strategy is specifically implemented in the way of taking a catalytic residue of zymophore as the center, selecting a high-flexibility residue 10 angstroms away from the center at most as a target locus according to the B-factor value in an enzyme crystal structure, and obtaining a mutant with enzyme thermodynamics stability improved through mutation screening. Under the guidance of the strategy, the thermal stability of candida rugosa lipase 1 with a highly complicated structure is improved remarkably. By means of stability change results of protein (LipA) with the smallest structure and moderately complicated protein (CalB), it is found that the success rate of enzyme mutation can be increased remarkably through mutation of residues with high B-factor 6-10 angstroms away from the catalytic residue. Meanwhile, through system analysis of a mutant structure-function relationship, a foundation is laid for deep research of enzyme region stabilization law and development of enzyme stabilization technique.

The present invention relates to the field of microbial remediation of cadmium contamination, and discloses an application method of a microbial inoculum capable of controlling a cadmium-polluted farmland by in-situ converting, and the microbial inoculum includes Acidiphilium cryptum, Candida rugosa, Acetobacter diazotrophicus, Rhodotorula glutinis and Pseudomonas aeruginosa. Through expanding culture of the microbial inoculum, the bacteria concentration reaches more than 1 x 10<9> / mL, he microbial inoculum is sprayed directly to the soil surface of the cadmium-polluted farmland by a small-dosage multi-time multi-point method, average 14-20L of the microbial inoculum liquid is applied to per square meter of the farmland, the soil and the repair microbial inoculum are evenly mixed by turning over, and the turning-over depth is 25-35cm. The application method of the microbial inoculum has the advantages of wide application range, repeated use of the microbial inoculum, large processing capacity, simple operation and low cost, can improve the conversion efficiency of the microbial inoculum to 50% or more, and has broad application prospects in the field of heavy metal contaminated soil remediation.

The invention provides a biologic deodorant as well as a preparation method and an application thereof. The biologic deodorant is a biologic fermentation liquid of which a microorganism strain is combined with one or more of yeast strains or one or two of bacteria into a mixed bacterium, wherein the yeast strains comprise candida Antarctica lipase and candida rugosa; the bacteria comprise bacillus licheniformis and bacillus subtilis; after microorganism strains are subjected to strain domesticating by using tea seed cake dregs as a sole nitrogen source, a two-stage fermentation process is adopted for fermentation, finally the microorganism strains in a fermentation bacterial liquid are in the dormant state, and the final biologic deodorant is obtained; when the biologic deodorant is used, after water is added and the biologic deodorant is in contact with air, the microorganism strains can continuously grow and generate more enzymes. By adopting the biologic deodorant provided by the invention, organic matters which can generate odor can be fundamentally removed, the degree that a pipeline is blocked by organic matters is reduced or eliminated, and the biologic deodorant can be also used for washing oil-containing or/and protein-containing or/and starch-containing dirt, does not damage any pipeline in a sewer, and is environmental-friendly.

The invention relates to the technical field of feed additive and processing thereof, and in particular to a probiotic feed additive. The probiotic feed additive is prepared by mixing autonomously separated lactobacillus plantarum, candida rugosa, candida ethanolica and lactococcus garvieae and buffalo milk fermentation substrate to ferment. The preparation process disclosed by the invention is simple, and the probiotic feed additive can be widely applied in the raising of livestock and poultry such as milk buffalos, pigs, chickens, cattle and sheep. The probiotic feed additive can remarkably improve the intestinal microbial floras of animals and enhance immunity, and can reduce the usage of antibiotic medicines and eliminate medicine residue, and when applied in production, the probiotic feed additive can shorten feeding periods and save feeds, thus increasing farming income.

The invention relates to a method for rapidly synthesizing ferulic acid glyceride through biologically catalyzing solvent-free system which comprises following steps: firstly, dissolving the ferulic acid and ethanol after reflux heating reation under catalysis of thionyl chloride, extracting sodium bicarbonate, removing ethyl ether, statically precipitating crystals, obtaining ferulic acid ethyl ester, and purifying, secondly, reacting the ferulic acid ethyl ester which is obtained and glycerin under the catalysis of candida rugosa lipase of biocatalyst and obtaining the ferulic acid glyceride. Compared with a traditional chemical method, the method for preparing the ferulic acid glyceride has temperate and safe reacting conditions and has the advantages of short reacting time, high productivity and the like, and simultaneously, the energy consumption and environmental pollution are reduced significantly since organic solvent is not used.

The present invention provides a Candida rugosa lipase mutant, wherein Candida rugosa (ATCC NO:14830) lipase LIP1 (CRL1) is subjected to thermal stability modification by using a site-directed mutation technology so as to optimize the practicality in the high temperature industrial environment. According to the present invention, the CRL1 gene is used as the template, the protein rational design technology is used, and the amino acid Asp at 4578 site of the CRL1 lipase gene sequence is substituted with Phe so as to obtain the lipase mutant with the improved thermal stability, wherein the application range and the catalytic efficiency of the enzyme are improved; and the CRL1 is subjected to site-directed mutation by using the molecular biology method to obtain the lipase mutant, wherein theTm value of the lipase mutant is increased by 9.4 DEG C, the optimum temperature is increased by 10 DEG C, and the T1/2 (50 DEG C) is 6.5 times the T1/2 (50 DEG C) of the parent lipase.

The invention belongs to the technical field of protein engineering and particularly relates to candida rugosa lipase 1 (Candida rugosa lipase1:LIP1) gene, wherein site-saturation mutagenesis is carried out at an active centre 414 site to obtain a series of mutants. The LIPI mutant for improving the heat stability in the invention can achieve effective catalytic activity at a high temperature, so that the application potential in the industry is improved; moreover, the primer selectivity of the mutant is prone to short chain acyl esters, and the LIPI mutant structurally uncovers the change mechanism.

The invention belongs to the technical field of chemical production, and relates to a production process for fermenting an organic fertilizer by using Maotai-flavor liquor vinasse. The production process comprises the following steps: (1) preparing a fermentation inoculant; (2) adding the fermentation inoculant in the step (1) into vinasse and auxiliary materials to prepare a fermentation raw material; and (3) transporting the fermentation raw materials obtained in the step (2) to a fermentation zone, and carrying out compost fermentation to obtain the organic fertilizer, the vinasse being Maotai-flavor liquor vinasse, and the fermentation inoculant being composed of Candida rugosa, Bacillus licheniformis, Paecilomyces variotii and Aspergillus fumigatus. According to the production process, the organic fertilizer production is performed on the Maotai-flavor Baijiu vinasse with characteristics of high acidity, high viscosity and degradation resistance under the conditions of natural pH,natural moisture and no additional chemical material adding, and the obtained organic fertilizer meets the national organic fertilizer standard (NY525-2012) and can be used for more scientifically and more effectively recycling and harmlessly utilizing the spent grains after the production of the Maotai-flavor liquor is finished, and has a wide application prospect in the Maotai-flavor liquor brewing industry.

The invention relates to a Decapsulated artemia egg preparation method improving the hatching rate, and belongs to the technical field of artemia eggs. The decapsulated artemia egg preparation method improving the hatching rate includes the steps: taking haw as the raw material, juicing the haw, and performing dydration on artemia eggs by means of the haw juice with organic acidity and light salt brine; and by coordination of bile and phosphatidase C, decomposing the phosphatide and cholesterol in the eggshells of the artemia eggs, and by coordination of Candida rugosa bacterium and the lipoprotein lipase and active substance secreted from the Candida rugosa bacterium, and papain, decapsulating the artemia eggs, wherein the egg envelopes of the decapsulated eggs have high resistance to papain and lipoprotein lipase so that the reaction can be terminated even no special inhibitor is used and the decapsulated artemia eggs can be obtained. Through the decapsulated artemia egg preparation method improving the hatching rate, the hatching rate of the obtained decapsulated artemia eggs is greatly improved when the decapsulated artemia eggs are hatched, and achieves 93-96%. Moreover, the decapsulated artemia egg preparation method improving the hatching rate is stable in the decapsulating effect and is low in the operating difficulty.

The invention relates to a method for preparing a propionic acid by synchronous bacterium and glycerin fermentation, which comprises that: (1) facultative aerobes are inoculated onto a culture medium to carry out aerobic culture, and anaerobic bacteria are inoculated onto the culture medium to carry out anaerobic culture; (2) the facultative aerobes cultured by the step (1) are inoculated onto a seed culture medium to carry out aerobic culture, and the anaerobic bacteria cultured by the step (1) are inoculated onto the seed culture medium to carry out the anaerobic culture; and (3) seed liquids of the facultative aerobes and the anaerobic bacteria are added in a fermentation medium to carry out anaerobic liquid fermentation to prepare the propionic acid, wherein the liquid seed culture medium for carrying out the anaerobic culture in the step (2) is added with glycerin to form the fermentation medium; and the anaerobic bacteria are propionibacterium acid propionicum 1.2225, and the facultative aerobes are candida rugosa.

The invention relates to a method for synthesizing ferulic acid glyceride in an organic solvent modified by a surface active agent, which comprises the steps: (1) methanol and activated carbon are used for purifying the surface active agent (AOT); (2) the purified AOT is used for modifying toluene which is a reaction medium; and (3) ferulic acid ethyl ester and triglycerides are catalyzed by the lipase of Candida rugosa in the toluene modified by the AOT, thus obtaining the ferulic acid glycerol. The method has mild and safe reaction condition and has the advantages of short reaction time, high yield rate, and the like; in addition, as the purity of the target product, namely the ferulic acid glyceride, does not require isolation and purification with down stream processing of bioengineering, thereby greatly lowering price and being more suitable for industrialized production.

The invention is suitable for the technical field of food processing, and provides frost-resistant oil and a preparation method thereof, chocolate and a preparation method of the chocolate. The preparation method of the frost-resistant oil comprises the steps of: carrying out tertiary fractionation treatment on shea butter by taking an organic solvent as a medium to obtain shea butter tertiary stearin; mixing the shea butter tertiary stearin with oil-soluble tea polyphenols, solid palm oil and a cocoa butter substitute evenly, wherein the mass ratio of the shea butter tertiary stearin to the oil-soluble tea polyphenols to the solid palm oil to the cocoa butter substitute is (26-37):(2-4):(3-5):(11-21); and adding 1.5-5.5% by mass of candida rugosa lipase, and carrying out enzymatic transesterification to obtain frost-resistant oil. The prepared frost-resistant oil is added to an existing chocolate food system, so that production of frost flowers in the chocolate food system can be effectively inhibited, and meanwhile, the color, taste and texture of the chocolate can also be improved to a certain extent.

The invention provides a method for preparing 3-hydroxypropionic acid by utilizing an immobilized candida rugosa cell. The method comprises the following steps: (1) preparing the immobilized candida rugosa cell; (2) inoculating the immobilized candida rugosa cell to a fermentation medium for shake cultivation; (3) filtering and separating the immobilized candida rugosa cell and the fermentation medium by sterile gauze after the cultivation is completed, adding a fresh fermentation medium with the same volume as the fermentation medium in the step (2) and culturing under the same condition as the step (2); (4) repeating the step (3), and terminating cultivation when the immobilized candida rugosa cell is completely ruptured; and (5) determining the content of 3-hydroxypropionic acid in the fermentation medium which is filtered and separated each time by utilizing high performance liquid chromatography. The preparation method is simple and convenient, has easily controlled operation program and high yield, is capable of overcoming the defect that a traditional non-immobilized strain and the fermentation medium are difficult to separate, and has the advantages of recycling, reducing cost and the like.

A method for producing biodiesel is provided, which includes providing a recombinant Candida rugosa lipase; reacting the recombinant C. rugosa lipase and a non-edible oil; and isolating the biodiesel from the reacted solution.

The invention discloses a sulfonated nano fibroin-cellulose mixed film and a preparation method thereof. Fibroin and cellulose acetate serve as raw materials, a nano fibroin-cellulose mixed film material is prepared through blending by using an electrostatic spinning technology, and the nano fibroin-cellulose mixed film material is chemically modified with sodium 3-sulfobenzoate to be prepared into the sulfonated nano fibroin-cellulose mixed film. According to the method, the green biomass fiber, namely fibroin fiber, and the cellulose acetate serve as the raw materials, the nano fibroin-cellulose mixed film material is prepared through blending by using the electrostatic spinning technology, then the material is chemically modified with sodium 3-sulfobenzoate to be prepared into the sulfonated nano fibroin-cellulose mixed film, the mixed film has a good adsorption effect on candida rugosa llipase, and finally the candida rugosa llipase is desorbed from the mixed film by adding neutralsalt into a solution to be recovered; and the repeated use performance of the mixed film is excellent.

Immobilization of Candida rugosa lipase on a carrier selected from the group consisting of macroporous adsorbent resin of the acrylic type, synthetic epoxy activated resin and Mg—Al-hydrotalcite enhances its enantioselectivity by six to seven folds. The immobilized Candida rugosa lipase is suitable for use in resolution of racemic alcohols and/or carboxylic acids, particularly in resolution of racemic menthol or production of menthyl esters.

The invention discloses a method for producing 3-hydracrylic acid by fermentation comprising inoculating candida rugosa seeds in a culture medium, stirring and controlling the pH value to 5.3+/-0.2; adding a outcome promoting factor, a sugar aqueous solution and a amino acid aqueous solution with a pH of 4.7+/-0.2; finally adding another sugar aqueous solution with a pH of 4.2+/-0.2, fermenting and culturing to obtain the fermentation solution containing 3-hydracrylic acid. The method of the invention employs a static optimization method combining a single factorial experiment, an orthogonal experiment and response surface experiment, optimizes the culture medium and the 3-hydracrylic acid production by pH sectional regulation, enables the yield of the 3-hydracrylic acid to be greatly improved to more than 28g/L, the conversion of the sugar to be 0.32g/g, the production ability to be 0.54g/Lh, and has an industrial popularization and application prospect.

The invention relates to a method for preparing R-6-hydroxy-1-aminoindane through dynamic kinetic resolution. The method is characterized by using candida rugosa lipase as a biological resolution catalyst, D-(-)-O-acetyl mandelic acid as an acyl donor and a non-crystal nickel catalyst KT-02 as a racemization catalyst to carry out one-pot dynamic kinetic resolution reaction in an autoclave in the presence of hydrogen to convert 6-hydroxy-1-aminoindane to an R-6-hydroxy-1-aminoindane acyl compound and carrying out acid hydrolysis, alkali freeing, and the like on the acyl compound to obtain R-6-hydroxy-1-aminoindane with ee (enantiomeric excess) value more than 99%. The method has the characteristics of simplicity in operation, cheap and accessible racemization catalyst, complete utilization of the raw materials, high optical purity of the product, and the like, and has great guidance and application values in production and preparation of R-6-hydroxy-1-aminoindane.

The invention discloses a preparation method of a posaconazole intermediate (III). The preparation method comprises the following steps of adopting an immobilized enzyme technology, and esterfying a compound (II) with isobutyric anhydride at the temperature of 35 to 40 DEG C in a weak acidic buffer under the catalysis of candida rugosa lipase, so as to obtain a mixture of posaconazole intermediates (II) and (III), and obtain the posaconazole intermediate (III) through quick column chromatography separation. By compared with a known invention of enzymic catalytic reaction, the method provided by the invention adopts the immobilized enzyme technology, the lipase can be recycled, the operation process is simple, and the cost is low. The posaconazole intermediate (III) obtained by the method provided by the invention can be used for the synthesis of posaconazole, and the ee value is larger than 99 percent.

The invention discloses a method for preparing R-6-methoxy-1-aminoindane through dynamic kinetic resolution. The method comprises the following steps: using 6-methoxy-1-aminoindane as a raw material, and under co-catalysis of a biological resolution catalyst, namely, candida rugosa lipase, and a racemization catalyst, namely, a non-crystal nickel catalyst KT-02, conducting transesterification reaction on 6-methoxy-1-aminoindane and an acyl donor, namely, (R)-(-)-alpha-acetylmandelic acid, to generate an R-6-methoxy-1-aminoindane acyl compound, and conducting acid hydrolysis and alkali ionization on the acyl compound to obtain R-6-methoxy-1-aminoindane, wherein the optical purity of the finished product is greater than 99%. The method has the characteristics that the operation is simple, the racemization catalyst is cheap and easy to obtain, the raw material utilization is complete, and the optical purity of the product is high. The method has great guidance and application values in preparation of R-6-methoxy-1-aminoindane.