Solvent-free system biological catalysis fast synthesis method for feruloylated glycerol

A ferulic acid glyceride, biocatalysis technology, applied in the direction of fermentation, etc., can solve the problems of high recovery and recycling costs, easy volatility, high toxicity, etc., and achieve the effect of shortening the reaction time, increasing the product yield, and fewer steps

Inactive Publication Date: 2008-07-09
DONGHUA UNIV
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Problems solved by technology

At the same time, it overcomes the shortcomings of organic solvents such as high toxicity, f...

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  • Solvent-free system biological catalysis fast synthesis method for feruloylated glycerol
  • Solvent-free system biological catalysis fast synthesis method for feruloylated glycerol

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Embodiment 1

[0029] (1) Substrate ferulic acid hydrophobization

[0030] 5g of ferulic acid and 50mL of absolute ethanol were catalyzed by 3mL of thionyl chloride, heated under reflux at 80°C and continuously stirred for 5h, and the reaction solution was rotatively evaporated to remove the solvent to obtain a light yellow oily substance, which was dissolved in a small amount of ether and washed with saturated bicarbonate The sodium solution was extracted for 3 times, and then the ether was removed by rotary evaporation, and the obtained liquid substance was allowed to stand still until a large amount of white needle-like crystals were precipitated to obtain ethyl ferulate.

[0031] (2) column chromatography purification of ethyl ferulate

[0032] Purification conditions: Packing: Take 25-30g of silica gel G, use ethyl acetate and benzene mixed solution as solvent for wet packing, and control the flow rate to 1 drop / s. Chromatographic conditions: using the method of elution in stages, th...

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Abstract

The invention relates to a method for rapidly synthesizing ferulic acid glyceride through biologically catalyzing solvent-free system which comprises following steps: firstly, dissolving the ferulic acid and ethanol after reflux heating reation under catalysis of thionyl chloride, extracting sodium bicarbonate, removing ethyl ether, statically precipitating crystals, obtaining ferulic acid ethyl ester, and purifying, secondly, reacting the ferulic acid ethyl ester which is obtained and glycerin under the catalysis of candida rugosa lipase of biocatalyst and obtaining the ferulic acid glyceride. Compared with a traditional chemical method, the method for preparing the ferulic acid glyceride has temperate and safe reacting conditions and has the advantages of short reacting time, high productivity and the like, and simultaneously, the energy consumption and environmental pollution are reduced significantly since organic solvent is not used.

Description

technical field [0001] The invention belongs to the field of ferulic acid glyceride preparation, in particular to a method for quickly synthesizing ferulic acid glyceride by biocatalysis in a solvent-free system. Background technique [0002] Ferulic acid (ferulic acid, FA) is a phenolic compound that exists in higher plants, and generally exists in the cell walls and insoluble fibers of corn, rice bran and bran. In recent years, ferulic acid has become a bulk biochemical product and has been widely used in medicine, medicine, cosmetics and other fields. Ferulic acid has anti-inflammatory, analgesic, anti-thrombosis, anti-ultraviolet radiation, inhibition of free radical production, endocrine regulation, and regulation of human immune function. It is clinically used for the treatment of diseases such as coronary heart disease, cerebrovascular disease, vasculitis, leukopenia and thrombocytopenia. Because of its strong absorption ability in the ultraviolet region of 280nm to...

Claims

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Application Information

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IPC IPC(8): C12P7/62
Inventor 朱利民郑妍权静蒋波
Owner DONGHUA UNIV
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