20 results about "Leukopenia" patented technology

The invention discloses a quadruple mRNA vaccine for preventing feline infectious diseases and application thereof, the quadruple mRNA vaccine can simultaneously prevent feline rabies, feline calicivirus disease, feline panleucopenia and feline rhinotracheitis through one-time immunization, and experiments show that the vaccine is relatively safe and strong in efficacy, the injection cost is reduced, and the vaccine is suitable for clinical application. The immune procedure is optimized, and the method has great significance in preventing cat infectious diseases.

The application discloses a norcantharidin derivative, a preparation method and application, and belongs to the technical field of medicine preparation. Norcantharidin and aminobenzene boronic acid are dissolved in a proper amount of dry tetrahydrofuran to obtain a crude product; ethyl acetate is added, and white solid is obtained through rotary evaporation under reduced pressure; ethyl acetate and acetone are added for stirring, and white powder norcantharidin derivative is obtained after filtration. The carboxyl of norcantharidin lactone after ring opening is connected with the amino of aminobenzene boronic acid to obtain norcantharidin derivative, which is combined with a chemotherapy drug or used as a preoperative drug to antagonize leukopenia caused by the chemotherapy drug. The norcantharidin derivative synthesized by the application has the advantages of simple method, high yield, low cost, remarkable effect of increasing white blood cells, effective reduction of the toxic side effect of norcantharidin, high oral bioavailability and improved curative effect.

The invention discloses a method for constructing a mammalian leucopenia model based on a Crispr gene editing technology. The invention belongs to the technical field of biology, and particularly relates to a method for constructing a mammalian leucopenia model based on a Crispr gene editing technology. The construction method of the leucopenia mouse model comprises the following steps: specifically mutating an H4K31 site in a mouse fertilized egg cell, so that the site is changed into arginine from lysine, and the leucopenia mouse model is obtained. A corresponding gene sequence is mutated at an H4K31 site in a fertilized egg cell of the specifically mutated mouse by means of cutting of a Crispr gene editing system and a fixed-point knock-in technology of homologous recombination. By detecting the immune function regulation and control mechanism of a homozygous mouse with the H4K31 site after the homozygous mouse with the H4K31 site is prepared, the mouse shows a natural leucopenia phenotype after lysine at the H4K31 site is mutated into arginine.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for preventing and treating leucopenia as well as a preparation method and application thereof. The composition is prepared from fructus momordicae, fructus ligustri lucidi, ginseng leaves and roses as raw materials through extraction. Experiments show that the composition can significantly increase the number and vitality of leukocytes so as to play multiple roles in increasing leukocytes, improving pathological injury of spleen tissues and enhancing immunity, and the effects are significantly superior to those of a formula-lacking control or a formula-replacing control. Therefore, all the components in the composition cooperate with one another and take effect synergistically, and are indispensable.

The invention relates to the technical field of pharmaceutical preparations, in particular to a traditional Chinese medicine composition for invigorating qi, blood, yin and yang and a preparation method and application thereof. The traditional Chinese medicine composition comprises the following raw materials: tortoise-shell glue, deer-horn glue, donkey-hide gelatin, ginseng, astragalus membranaceus, codonopsis pilosula and fructus lycii, and is prepared by fermenting the raw materials, and the mass ratio of the tortoise-shell glue to the deer-horn glue to the donkey-hide gelatin to the ginseng to the astragalus membranaceus to the codonopsis pilosula to the fructus lycii is 1: (0.5-2): (0.5-2): (1-4): (2-5): (2-6): (2-6). The fermented product obtained by fermenting the medicine raw materials through probiotics can be used for treating qi-blood yin-yang deficiency syndromes such as fatigue, myocardial ischemia, cerebral hypoxia and kidney essence deficiency and / or treating and preventing leukopenia caused by myelosuppression and / or DNA (deoxyribonucleic acid) reduction after radiation and / or hypoimmunity and plateau hypoxia. The effects of preventing sudden cardiac death and the like are obvious.

This invention discloses a uric acid-lowering drug composition, which comprises one or more of the following: levocarnitine, levocarnitine derivatives, pharmaceutically acceptable salts of levocarnitine or its derivatives, glucocorticoids, colchicine, nonsteroidal anti-inflammatory drugs, IL-1β inhibitors, drugs that inhibit uric acid production, drugs that promote uric acid excretion, and uricase. The composition has the effects of lowering uric acid, protecting liver and kidney function, and inhibiting leukopenia.

The invention relates to the technical field of pharmaceutical preparations, and provides a pharmaceutical composition for preventing and / or treating leucopenia caused by chemotherapy and a preparation method of the pharmaceutical composition. The pharmaceutical composition is prepared from the following raw materials: red ginseng, prepared rehmannia root, morinda officinalis, pericarpium citri reticulatae, semen cuscutae, rhizoma atractylodis macrocephalae, Chinese yam, astragalus membranaceus, poria cocos, radix achyranthis bidentatae, cistanche, cinnamon, angelica sinensis, fructus lycii, cornu cervi pantotrichum, fennel, radix paeoniae alba and liquorice. The pharmaceutical composition provided by the invention realizes prevention and / or treatment of leucopenia caused by chemotherapy, specifically, the pharmaceutical composition is combined with the chemotherapeutic drug 5-fluorouracil for use, and is applied before, during or after chemotherapeutic drug administration, and the leucocyte level of a subject is increased by promoting lymphocyte and / or monocyte proliferation.

The invention relates to the technical field of traditional Chinese medicine, and provides a medicine for treating radiotherapy-induced leucopenia and a preparation method and application thereof.The medicine comprises a traditional Chinese medicine composition, and the traditional Chinese medicine composition comprises ginseng, rhizoma atractylodis macrocephalae fried with bran, poria cocos, Chinese yam, pericarpium citri reticulatae, radix aucklandiae, fructus amomi, radix astragali preparata, angelica sinensis, fried semen ziziphi spinosae and processed polygala tenuifolia. When the traditional Chinese medicine composition acts on mammals, it is verified that the traditional Chinese medicine composition has a good curative effect on leucopenia and / or bone marrow hematopoietic tissue area reduction caused by radiotherapy, and the level of pro-inflammatory cytokines increased due to radiotherapy in serum of the mammals is lowered.

The application discloses a feline panleukopenia virus, feline herpes virus and feline calicivirus yolk antibody preparation and a preparation method and application thereof. The feline triple yolk antibody preparation comprises a core material, and the core material comprises feline calicivirus yolk antibody, feline panleukopenia virus yolk antibody, feline herpes virus type 1 yolk antibody and probiotics. The application can produce specific neutralization to feline calicivirus, feline panleukopenia virus and feline herpes virus type 1, significantly reduces the infection rate and the severity of clinical symptoms, is stably released in the gastrointestinal tract after oral administration, the antibody activity retention rate is more than 90%, the probiotics synergistically regulate intestinal microecology, enhance the immune response of the body and improve the overall protection efficiency.

This invention relates to the field of biomedicine. It provides a compound astragalus and ganoderma preparation with anti-cancer properties, its preparation method, and its application. The preparation is made from the following raw materials in parts by weight: 200-500 parts ganoderma, 200-500 parts astragalus, 50-100 parts ganoderma spore powder, 30-60 parts Panax notoginseng, 30-60 parts Salvia miltiorrhiza, 50-100 parts Atractylodes macrocephala, 150-300 parts Smilax glabra, 100-200 parts Hedyotis diffusa, 20-40 parts Bombyx mori, and 20-40 parts Glycyrrhiza uralensis. The compound astragalus and ganoderma preparation of this invention has anti-tumor effects and improves the effects of radiotherapy and chemotherapy on leukopenia and thrombocytopenia, as well as adverse reactions caused by anti-tumor treatment.

A growing number of patients presenting severe combined immunodeficiencies associated with Rac2 mutations have been identified in newborns. These mutations are associated with high frequency of infection, leukopenia and more rarely autoinflammatory features. Here, the inventors show that the Rac2 activating mutations activates the NLRP3 inflammasome leading to the secretion of IL-1β from macrophages depending on their activation strength. Therefore, inhibiting GasderminD (GSDMD) pathway should be considered as a potential treatment for these patients. The present invention relates to a method of treating Rac2 monogenic disorders in a subject in need thereof comprising the administration of an inhibitor of GasderminD (GSDMD).

The present invention discloses a traditional Chinese medicine formula for treating post-chemotherapy bone marrow suppression, as well as a preparation method and application thereof, belonging to the technical field of traditional Chinese medicine. The traditional Chinese medicine formula of the present invention is prepared from ten traditional Chinese medicines: Millettia reticulata, Clove, Astragalus, Cimicifuga heracleifolia, Sanguisorba officinalis, Agrimoniae scabra, Rhodiola rosea, Codonopsis pilosula, Cinnamon bark, and Angelica sinensis, and has a significant effect on improving post-chemotherapy bone marrow suppression. The present invention also has good therapeutic effects on four types of post-chemotherapy bone marrow suppression, including leukopenia, neutropenia, thrombocytopenia, and low hemoglobin. The formula has good stability, high safety, and significant efficacy.

The application discloses a norcantharidin derivative, a synthetic method and application thereof, and belongs to the technical field of medicine preparation. Norcantharidin and aminobenzene boronic acid are dissolved in an ice acetic acid solution, after reaction, the obtained white filter residue is dried to obtain a crude product; then the crude product is dissolved in methanol and repeatedly recrystallized to obtain transparent needle-like crystalline norcantharidin derivative. The amino group of aminobenzene boronic acid is used to connect norcantharidin and aminobenzene boronic acid to obtain the norcantharidin derivative, which can antagonize leukopenia caused by chemotherapy drugs on the basis of retaining the anticancer activity. The synthetic method is simple, the yield is high, the cost is low, the toxic and side effects of norcantharidin in treating liver cancer can be effectively reduced, the oral bioavailability is high, and the curative effect is improved.

The invention is suitable for the technical field of extraction and preparation of natural active ingredients, and provides a preparation method of dendrobium germ glycopeptide and application of the dendrobium germ glycopeptide in treatment of leucopenia. The preparation method of the dendrobium germ glycopeptide comprises the following steps: pretreatment of raw materials: selecting dendrobium officinale germs, and crushing the dendrobium officinale germs to prepare a germ suspension; low-temperature composite enzymatic hydrolysis: adding protein powder and compound enzyme into the germ suspension, performing constant-temperature enzymatic hydrolysis at 40-45 DEG C, after the enzymatic hydrolysis is finished, heating the system to 85-90 DEG C to inactivate the enzyme, cooling to room temperature, centrifuging and collecting supernate to obtain crude enzymatic hydrolysate; gradient purification: carrying out deproteinization treatment and ultrafiltration separation on the crude enzymatic hydrolysate to obtain a refined glycopeptide mixed solution; directional compounding and drying: adding sodium citrate into the refined glycopeptide mixed solution to complete directional compounding of polysaccharide and peptide, and then freeze-drying to obtain a dendrobium germ glycopeptide finished product. The method solves the problems of low activity, poor purity and single effect in the extraction of the active ingredients of the dendrobium germs in the prior art.

The present disclosure relates generally to complexes comprising an interleukin-15 (“EL-15”) polypeptide chain non-covalently bonded to an IL-15 receptor α-Fc fusion polypeptide (“IL-15Rα-Fc”), and multimers (e.g., trimers) thereof. The complexes of the present technology are useful in methods for enhancing immune cell function and immunotherapy in a patient in need thereof. Also provided herein are methods for preventing or treating a disease or condition in which enhancing IL-15-mediated function is beneficial (such as cancer, infectious diseases, immunodeficiencies and leukopenia) in a patient in need thereof.

The present invention relates to the use of a formulation comprising ozonized oil for the prevention or reduction of the side effects of chemotherapy or radiotherapy, in particular for the prevention of leukopenia, anaemia or thrombocytopenia caused by said anti-tumour treatments.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for preventing and treating myelosuppression and leucopenia caused by tumor chemoradiotherapy and a preparation method and application of the traditional Chinese medicine composition. Comprising the following raw material medicines in parts by weight: 1-45 parts of astragalus membranaceus, 1-45 parts of rhizoma polygonati, 1-45 parts of radices trichosanthis, 1-45 parts of scalded rhizoma drynariae, 1-30 parts of wine-processed glossy privet fruit, 1-30 parts of roasted herba epimedii, 1-30 parts of radix pseudostellariae, 1-30 parts of bighead atractylodes rhizome, 1-20 parts of vinegar-processed radix bupleuri and 1-15 parts of pericarpium citri reticulatae. The composition disclosed by the invention not only can effectively increase the leukocyte count of a patient after radiotherapy and chemotherapy, but also can remarkably improve spleen-kidney deficiency related clinical symptoms, such as soreness and weakness of waist and knees, fatigue, intolerance of cold and cold limbs, reduced appetite and loose stool, accompanied by the patient, so that the dual goals of increasing leukocyte and improving life quality are achieved, and the defect that a traditional treatment medicine treats both symptoms and root causes is overcome.

This invention relates to a traditional Chinese medicine composition for treating toxic diffuse goiter, the composition being made from the following raw materials in parts by weight: Gardenia jasminoides 7-11 parts, vinegar-processed turtle shell 7-11 parts, processed Pinellia ternata 7-11 parts, Bupleurum chinense 7-11 parts, and Panax notoginseng 4-8 parts. This invention also provides applications of the traditional Chinese medicine composition. This invention invigorates blood circulation, disperses stagnation, clears heat, and eliminates goiter. This formula uses Bupleurum chinense as the chief ingredient, combined with Gardenia jasminoides to clear stagnation and guide fire, and processed Pinellia ternata to dispel dampness and resolve phlegm; turtle shell, a yin-strengthening herb, tonifies kidney yin and clears heart fire. Furthermore, there is the theory that prolonged illness often leads to blood stasis, obstructing the meridians, with qi, phlegm, fire, and blood stasis intertwined; Panax notoginseng is added to invigorate blood circulation, unblock the meridians, disperse stagnation, and eliminate masses to interrupt the process. The combination of these herbs clears and drains heart and liver fire, directly addressing the pathogenesis. This invention, combined with a small dose of antithyroid drugs, leads to faster improvement in thyrotoxicosis symptoms. It also improves the patient's depression and anxiety, prevents liver damage and leukopenia, and significantly reduces side effects.

The invention provides chimeric virus-like particles for feline infectious rhinoconjunctivitis, feline infectious rhinotracheitis and feline panleucopenia, a triple vaccine and a preparation method and application of the chimeric virus-like particles and the triple vaccine, and belongs to the technical field of biological products. The invention provides chimeric virus-like particles assembled by feline parvovirus-like particles and feline herpesvirus type I gD protein and chimeric virus-like particles assembled by feline calicivirus virus-like particles and feline herpesvirus type I gB protein. The invention also provides a triple vaccine which is prepared from the chimeric virus-like particle and an adjuvant. Experiments prove that the chimeric virus-like particle triple vaccine has good safety and antigen compatibility, no local or whole-body adverse reaction occurs after animals are immunized, and a body can be induced to generate good humoral immune and cellular immune responses. Therefore, the chimeric virus-like particle triple vaccine has the advantages of good immunogenicity, high safety, low production cost and the like.