37 results about "Antibody activity" patented technology

This invention discloses a camel-derived CRP single-domain antibody, its preparation method, and its applications, relating to the biomedical field. The method involves enriching CRP antigen in alpaca leukocytes immunized with the antigen, followed by single-cell screening and sequencing. Through sequencing structural analysis, a recombinant plasmid of the camel-derived CRP antibody is constructed. This plasmid is cloned and transformed into eukaryotic cells to express related antibodies. Monoclonal antibodies with human CRP resistance are screened for expression. The VHH domain of the monoclonal antibody is cloned and expressed, and paired screening yields CRP-VHH monoclonal antibodies with antibody activity. This antibody can be used in ELISA kits, chemiluminescence kits, and latex kits, possessing the same functionality as existing kits. Furthermore, this antibody also has applications in the preparation of antitumor drugs.

This disclosure provides anti-VEGF antibodies formulated as high-concentration aqueous pharmaceutical compositions suitable for injection, preferably intravitreal injection. Aqueous pharmaceutical compositions are useful for delivering a high concentration of the antibody active ingredient to a patient without high levels of antibody aggregation and without a high level of sub-visible particulate matter. An aqueous composition of the disclosure comprises an antibody having a concentration of at least 50 mg / mL. An aqueous pharmaceutical composition of the disclosure includes a sugar, a buffering agent, and a surfactant.

Compositions and methods for modulating antibody activity are disclosed.

The application discloses a CRP camel-derived single-domain antibody and a preparation method and application thereof, relates to the field of biological medicines, and is characterized in that: white blood cells of a llama immunized with a CRP antigen are enriched, and then single-cell screening and sequencing are performed. Through sequencing structure analysis, a camel-derived CRP antibody recombinant plasmid is constructed, a related antibody is expressed in a eukaryotic cell through cloning transformation, a single antibody with human CRP resistance is screened, a VHH domain of the single antibody is cloned and expressed, and a single antibody with CRP-VHH antibody activity is obtained through pairing screening. The antibody can be applied to an ELISA kit, a chemiluminescence kit and a latex kit, has the same functions as existing kits, and still has the use in the preparation of an anti-tumor drug.

The invention relates to a glycosylated hemoglobin HbA1c freeze-drying detection reagent and a preparation method and application thereof.The preparation method comprises the steps that surface aminated polystyrene microspheres are prepared through an emulsion polymerization method, then double bonds are introduced through Schiff base reaction and methylacrolein reaction, and the glycosylated hemoglobin HbA1c freeze-drying detection reagent is obtained. Then preparing anionic polystyrene microspheres with hydrophilic chains through free radical polymerization, and preparing freeze-dried powder of the HbA1c detection reagent R1 by adopting the anionic polystyrene microspheres; the preparation method comprises the following steps: preparing a hydrophilic cross-linking agent 2, 5-bis (2, 4-dihydroxy-6-formylphenoxy) terephthalaldehyde, then carrying out a Schiff base reaction on the hydrophilic cross-linking agent 2, 5-bis (2, 4-dihydroxy-6-formylphenoxy) terephthalaldehyde and HbA1c mAb to prepare an HbA1c oligomer, and preparing the HbA1c detection reagent R2 freeze-dried powder by using the HbA1c oligomer. The glycosylated hemoglobin HbA1c freeze-drying detection reagent prepared by the invention has the advantages of high antigen binding efficiency, excellent detection sensitivity, sufficient retention of antibody activity and strong detection specificity.

The invention relates to the technical field of antibody titer digitization analysis, in particular to a brucellosis antibody titer digitization analysis system based on a spectrophotometric method.According to the brucellosis antibody titer digitization analysis system, a full-time reaction kinetics absorbance curve is obtained through a data acquisition module; a signal decomposition module is utilized to decouple the signal into a low-frequency trend component and a high-frequency detail component; further identifying a reaction kinetics phase state through a low-frequency trend analysis module to lock an effective reaction interval and generate first-dimension analysis data, and controlling a high-frequency detail analysis module to generate second-dimension analysis data by taking the first-dimension analysis data as a time domain gating basis; finally, the comprehensive evaluation module constructs a coupling weight model based on the reaction kinetics phase state, and fuses the two-dimensional data to generate a comprehensive antibody activity evaluation value; according to the process, a self-adaptive analysis mechanism conforming to a reaction evolution rule is constructed, and accurate determination of the titer of the antibody is realized.

This invention provides a method for producing PD-1 antibodies with high protein expression levels, high antibody activity, and / or high galactosyl levels. This method involves culturing cells in a culture medium until the target protein is obtained. The culture temperature is 37°C, which is then lowered to 33-34°C on the 5th day of culture. The culture medium also contains additives, namely galactose and manganese chloride. The concentrations of galactose and manganese chloride added are 0.05-6 mM and 0.05-3 μM, respectively. The culture medium consists of a basal medium and a feed medium. The basal medium is Star CHO, and the feed medium is Star CHO Feed and CDFS36. The basal medium also contains 1 g / L poloxamer. The Star CHO Feed feed is added at 4% / 5% / 6% / 6% / 5% / 4% of the initial culture volume each time, and the CDFS36 feed is added at 0.4% / 0.5% / 0.6% / 0.6% / 0.5% / 0.4% of the initial culture volume each time. The additives galactose and manganese chloride, as well as the Star CHO feed feed... Feed and CDFS36 were added every 2 days starting from day 3 of culture; the dissolved oxygen in the culture system was 40%, and the pH was 7.0 ± 0.1; the rotation speed of the culture system was 240 rpm.

The invention provides an anti-tetanus antibody as well as preparation and application thereof, and relates to the field of antibodies. The amino acid sequence of the tetanus-resistant antibody heavy chain CDR is as shown in SEQ ID NO: 1-3; the amino acid sequence of the light chain CDR of the antibody is as shown in SEQ ID NO: 4-6. The antibody disclosed by the invention is high in activity and affinity, and has the application prospect of preparing a medicine for preventing and / or treating tetanus or a product for detecting the tetanus toxin level with a non-diagnostic purpose and the like.

The invention discloses a retinol binding protein detection kit and a preparation method thereof. The detection kit comprises a reagent R1 and a reagent R2. The reagent R1 comprises an oxidizing agent and is beneficial to maintaining the structural stability of the retinol binding protein. And the R2 reagent comprises latex microspheres loaded with a retinol binding protein antibody. According to the present invention, the antibody directional coupling technology is adopted, such that the stable thioether bond is formed between the retinol binding protein antibody and the latex microsphere, the retinol binding protein antibody is coupled to the latex microsphere, the antibody is connected with the microsphere through the Fc region, and the Fab region extends outward so as not to affect the antibody active region configuration, and the efficient combination with the sample antigen is achieved;

The invention relates to a humanized antibody of a dermatomyositis specific antigen as well as a preparation method and application of the humanized antibody. Amino acid sequences of HCDR1, HCDR2 and HCDR3 of a heavy chain of the antibody respectively comprise sequences as shown in SEQ ID No.6 to SEQ ID No.8, and amino acid sequences of LCDR1, LCDR2 and LCDR3 of a light chain of the antibody respectively comprise a sequence as shown in SEQ ID No.9, a sequence as shown in GQD and a sequence as shown in SEQ ID No.10. The anti-MDA5 human monoclonal antibody obtained through screening is high in activity, good in stability and high in MDA5 binding specificity, can serve as a reference standard for qualitative detection of MDA5 positive, can also be used for quantitative detection of the anti-MDA5 autoantibody level in patients suffering from inflammatory myopathy or complications of the inflammatory myopathy, and can be used for clinical application. And a new tool is provided for diagnosis, monitoring, treatment and the like of dermatomyositis and complications thereof.

This invention discloses a Pt-Ni@erGO electrochemical probe, its preparation method, and its applications. The Pt-Ni@erGO electrochemical probe comprises an erGO matrix and Pt-Ni nanoparticles supported on the erGO matrix. The Pt-Ni@erGO electrochemical probe of this invention uses erGO as a matrix to grow Pt-Ni alloy nanoparticles in situ. erGO simultaneously exhibits two functions to enhance electrochemical detection sensitivity: firstly, it acts as a carrier to load high-density Pt-Ni alloy nanoparticles, reducing the shielding effect of biorecognition elements on the catalytic sites of nanozymes; secondly, it acts as a biorecognition element to specifically bind to *E. coli*. Through the rational combination of erGO and Pt-Ni nanoparticles, the obtained Pt-Ni@erGO possesses high antibody-like activity and electrocatalytic activity.

The present application relates to the technical field of in vitro diagnosis protein marker, in particular to a preservative for horseradish peroxidase marker and application thereof. The present application provides a preservative for horseradish peroxidase marker and application thereof. The preservative comprises lecithin 0.05-1.0 g / L, bovine serum albumin 2-10 g / L, sodium chloride 5-30 g / L, polyaspartic acid 2-10 g / L, and the rest is glycerol. The preservative can significantly improve the storage stability of enzyme-labeled product, and the activity of enzyme-labeled antibody can still maintain more than 70% after 21 days of storage at 37 DEG C. In the bottle opening stability test, the activity of enzyme-labeled antibody can maintain more than 87% after 45 days of storage at 4 DEG C.

The invention discloses a monkey pox virus B6-A29 and A35-M1 fusion antigen and application thereof, and belongs to the technical field of biological medicine. The B6-A29 and A35-M1 fusion protein with good stability and uniform quality is designed, the neutralizing antibody activity is remarkably enhanced, the in-vivo protection effect is good, and the long-term protection effect is achieved. Compared with simple superposition of a single protein mixing effect, the method has better humoral immunity and cellular immunity, and the production, purification and quality control processes are simpler, more stable and lower in cost compared with a scheme of respectively producing four independent proteins and then mixing, and more accord with the requirements of vaccine industrial production.

The invention discloses a conjugate of phosphorylated Tau protein and magnetic beads as well as a preparation method and a kit of the conjugate, and relates to the technical field of immunoassay. The modified magnetic beads modified with azide groups and the modified anti-phosphorylated Tau protein antibody modified with DBCO groups are efficiently and directionally coupled in a click chemistry mode, and compared with a traditional physical adsorption or non-specific chemical coupling method, the method has the advantages of being high in coupling efficiency, high in binding stability, high in antibody activity retention rate and the like; compared with the prior art, the magnetic bead provided by the invention has the advantages that the capture efficiency and specificity of the magnetic bead on low-abundance p-Tau in blood can be obviously improved, the lower detection limit is as low as 0.3 pg / mL or below, excellent accuracy is shown in early AD diagnosis, subtle changes of p-Tau in the blood of an early AD patient can be accurately identified, and a reliable tool is provided for early screening, disease course monitoring and intervention effect evaluation of AD.

The application relates to an antigen binding protein, an antibody or an antibody active fragment obtained by immunizing a camelid with a Sumo tag protein. The application relies on the immune system of the camelid to screen, identify and prepare an antibody specifically recognizing and binding the Sumo tag protein. The obtained antibody has high specificity and can be used for target antigen detection, and has potential clinical diagnosis and treatment values. The antibody provided by the application has simple structure, is easy to be genetically engineered, is easy to be humanized, has high stability, has low mass production cost, and is beneficial to realize large-scale production.

The application discloses a feline panleukopenia virus, feline herpes virus and feline calicivirus yolk antibody preparation and a preparation method and application thereof. The feline triple yolk antibody preparation comprises a core material, and the core material comprises feline calicivirus yolk antibody, feline panleukopenia virus yolk antibody, feline herpes virus type 1 yolk antibody and probiotics. The application can produce specific neutralization to feline calicivirus, feline panleukopenia virus and feline herpes virus type 1, significantly reduces the infection rate and the severity of clinical symptoms, is stably released in the gastrointestinal tract after oral administration, the antibody activity retention rate is more than 90%, the probiotics synergistically regulate intestinal microecology, enhance the immune response of the body and improve the overall protection efficiency.

The present invention belongs to the field of biomedicine, and in particular to a humanized anti-human CD132 monoclonal antibody and its application. Based on the monoclonal chimeras 2D4 and 5H10 previously obtained in this laboratory, the present invention screened and obtained high-affinity monoclonal humanized antibodies h2D4H4K12 and / or h5H10H6K4 that can target human CD132 by humanizing the target antibody. On the basis of not weakening the antibody activity, the immunogenicity of the maternal chimera was reduced, and the risk that the patient using the drug may produce an immune response to the antibody was further reduced. It has good application prospects in the treatment of autoimmune diseases (such as rheumatoid arthritis, systemic lupus erythematosus, etc.) associated with IL-4, IL-7, IL-9, IL-15 and / or IL-21 cytokines.

The present application relates to a kind of anti-MDA5 humanized antibody and its preparation method and application.The amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain of the antibody respectively include the sequences shown in SEQ ID NO.1~SEQ ID NO.3, the amino acid sequences of CDR1, CDR2 and CDR3 of light chain respectively include the sequence shown in SEQ ID NO.4, QDN and the sequence shown in SEQ ID NO.5.The present application obtains anti-MDA5 human monoclonal antibody by screening, the activity of the antibody is high, stability is good, and has the specificity of stronger binding MDA5, provides new tool for dermatomyositis and its complication diagnosis, monitoring and treatment etc..

The application discloses a CRP camel-derived single-domain antibody and a preparation method and application thereof, relates to the field of biological medicines, and is characterized in that: white blood cells of a llama immunized with a CRP antigen are enriched, and then single-cell screening and sequencing are performed. Through sequencing structure analysis, a camel-derived CRP antibody recombinant plasmid is constructed, a related antibody is expressed in a eukaryotic cell through cloning transformation, a single antibody with human CRP resistance is screened, a VHH domain of the single antibody is cloned and expressed, and a single antibody with CRP-VHH antibody activity is obtained through pairing screening. The antibody can be applied to an ELISA kit, a chemiluminescence kit and a latex kit, has the same functions as existing kits, and still has the use in the preparation of an anti-tumor drug.

The application relates to a glycosylated hemoglobin HbA1c freeze-drying detection reagent and a preparation method and application thereof, the preparation method comprising the following steps: preparing surface-aminoated polystyrene microspheres through an emulsion polymerization method, then introducing double bonds through a Schiff base reaction with methyl acrolein, and then preparing anion polystyrene microspheres with hydrophilic chains through free radical polymerization, preparing HbA1c detection reagent R1 freeze-dried powder by using the anion polystyrene microspheres; preparing a hydrophilic crosslinking agent 2,5-bis(2,4-dihydroxy-6-formyl phenoxy) p-phenylenedimethylene, and then preparing HbA1c oligomers by a Schiff base reaction with HbA1c mAb, and preparing HbA1c detection reagent R2 freeze-dried powder by using the HbA1c oligomers. The glycosylated hemoglobin HbA1c freeze-drying detection reagent prepared by the application has high antigen binding efficiency, excellent detection sensitivity, sufficient antibody activity reservation and strong detection specificity.

The invention relates to an anti-MDA5 humanized antibody as well as a preparation method and application thereof. The amino acid sequences of CDR1, CDR2 and CDR3 of a heavy chain of the antibody respectively comprise sequences as shown in SEQ ID NO.1-SEQ ID NO.3, and the amino acid sequences of CDR1, CDR2 and CDR3 of a light chain of the antibody respectively comprise a sequence as shown in SEQ ID NO.4 and sequences as shown in QDN and SEQ ID NO.5. The anti-MDA5 human monoclonal antibody is obtained through screening, the antibody is high in activity and good in stability, the specificity for combining MDA5 is high, and a new tool is provided for diagnosis, monitoring, treatment and the like of dermatomyositis and complications of dermatomyositis.

The present invention discloses a monoclonal antibody against c-Myc tag and its application. The antibody comprises a heavy chain CDR1, a heavy chain CDR2 and a heavy chain CDR3, and a light chain CDR1, a light chain CDR2 and a light chain CDR3; the amino acid sequence of the heavy chain CDR1 is shown in SEQ ID NO: 1; the amino acid sequence of the heavy chain CDR2 is shown in SEQ ID NO: 2; the amino acid sequence of the heavy chain CDR3 is shown in SEQ ID NO: 3; the amino acid sequence of the light chain CDR1 is shown in SEQ ID NO: 4; the amino acid sequence of the light chain CDR2 is shown in SEQ ID NO: 5; and the amino acid sequence of the light chain CDR3 is shown in SEQ ID NO: 6; the ascites antibody titer thereof can reach 8.48×10 6 After purification, the antibody affinity reached 1.113E‑8, the antibody activity was high, and it has great application potential.

The invention relates to an enzyme conjugate diluent and application thereof, and belongs to the technical field of biological in-vitro detection reagents. The enzyme conjugate diluent comprises bovine serum albumin, fetal calf serum, polyethylene glycol 20000, casein amino acid and a buffer solution. In the enzyme conjugate diluent, the concentration of bovine serum albumin is 2-4 wt%, the concentration of polyethylene glycol 20000 is 1.2-3.6 wt%, and the concentration of casein amino acid is 1.6-4.8 wt%. The enzyme conjugate diluent disclosed by the invention has an excellent protection effect on an enzyme conjugate, can effectively stabilize numerical values in detection, can effectively ensure the result stability and antibody activity of the enzyme conjugate in a room temperature or more extreme high-temperature environment, can keep the stability of an enzyme-labeled antibody for a longer time in a non-refrigeration environment, and has the characteristics of simple preparation, low cost and the like. And the kit has the characteristics of high cost performance, good preservation effect and the like, and provides a reagent basis for the stability of enzyme-labeled detection.

The invention relates to an MDA5 targeting antibody as well as a preparation method and application thereof. The anti-MDA5 human monoclonal antibody is obtained through screening, the antibody is high in activity and good in stability, the specificity for combining MDA5 is high, and a new tool is provided for diagnosis, monitoring, treatment and the like of dermatomyositis and complications of dermatomyositis.

The application relates to an antibody targeting MDA5 and a preparation method and application thereof. A human monoclonal antibody targeting MDA5 is screened, the antibody has high activity, good stability, and high specificity in binding MDA5, and provides a new tool for the diagnosis, monitoring and treatment of dermatomyositis and its complications.

The present application relates to a kind of V5 label antibodies and its application, the antibody includes heavy chain CDR1, heavy chain CDR2 and heavy chain CDR3, and light chain CDR1, light chain CDR2 and light chain CDR3;The amino acid sequence of the heavy chain CDR1 is as shown in SEQ ID NO:1;The amino acid sequence of heavy chain CDR2 is as shown in SEQ ID NO:2 sequence;The amino acid sequence of heavy chain CDR3 is as shown in SEQ ID NO:3 sequence;The amino acid sequence of light chain CDR1 is as shown in SEQ ID NO:4 sequence;The amino acid sequence of light chain CDR2 is as shown in SEQ ID NO:5;The amino acid sequence of light chain CDR3 is as shown in SEQ ID NO:6;Its ascites antibody titer can reach 2.07×10 6 , after purification antibody affinity reaches 1.425E-8, antibody activity is high, with great application potential.

The invention relates to a carbon-based biosensor, a preparation method thereof and a non-invasive detection method in PD-1, and solves the problems of direct fixation of an antibody and rapid detection of human-mouse source PD-1. And the channel material terminating electrode forms low ohmic contact which is directly used as a sensing interface. The preparation comprises design; performing cleaning; preparing an electrode; and depositing the s-SWCNTs to finish the preparation. In the detection, quantitative detection of PD-1 is realized mainly by fixing a PD-1 monoclonal antibody site and specifically binding with PD-1 protein. According to the invention, the CNT-FET is used for non-invasive and rapid real-time detection of human and mouse source PD-1 for the first time, and s-SWCNTs ends are connected to the upper surfaces of the source electrode and the drain electrode; the preparation process of the first electrode and the second CNT directly fixes the antibody through electrostatic adsorption without modification, so that the activity of the antibody is ensured. Sensitivity is high, process is simple and cost is low; the kit is used for PD-1 detection in NSCLC immunotherapy, and can also be used for multi-index synchronous detection and bedside detection.

This invention discloses a camel-derived CRP single-domain antibody, its preparation method, and its applications, relating to the biomedical field. The method involves enriching CRP antigen in alpaca leukocytes immunized with the antibody, followed by single-cell screening and sequencing. Through sequencing structural analysis, a recombinant plasmid of the camel-derived CRP antibody is constructed. This plasmid is cloned and transformed into eukaryotic cells to express related antibodies. Monoclonal antibodies with human CRP resistance are screened for expression. The VHH domain of the monoclonal antibody is cloned and expressed, and paired screening yields CRP-VHH monoclonal antibodies with antibody activity. This antibody can be used in ELISA kits, chemiluminescence kits, and latex kits, possessing the same functionality as existing kits. Furthermore, this antibody also has applications in the preparation of antitumor drugs.