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Method for preparing yolk immunoglobulin vaccine for resisting porphyromonas gingivalis

A technology of yolk immunoglobulin and porphyrin gingivalis is applied in the field of preparation of anti-Porphyromonas gingivalis yolk immunoglobulin vaccine, which can solve the problems of bacterial resistance, toxic and side effects, and achieve good compliance and stability. , The effect of purification is simple and quick

Active Publication Date: 2010-08-04
ANHUI MEDICAL UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, frequent administration of antibiotics can easily lead to bacterial resistance and has toxic side effects

Method used

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  • Method for preparing yolk immunoglobulin vaccine for resisting porphyromonas gingivalis
  • Method for preparing yolk immunoglobulin vaccine for resisting porphyromonas gingivalis
  • Method for preparing yolk immunoglobulin vaccine for resisting porphyromonas gingivalis

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Embodiment

[0037] The preparation method of anti-Porphyromonas gingivalis yolk immunoglobulin vaccine is characterized in that comprising the following steps:

[0038] (1) Culture and identification of Porphyromonas gingivalis

[0039] After fully dissolving the international standard strain of Porphyromonas gingivalis ATCC 33277 with 0.2ml of sterile phosphate buffered saline (PBS, PH7.2), inoculate it on bovine brain heart infusion (BHI) sheep blood agar solid medium, 37 ℃, 80% nitrogen (N 2 ), 10% carbon dioxide (CO 2 ), 10% hydrogen (H 2 ) anaerobic culture for 7 to 10 days. Observe the colony morphology for identification. Porphyromonas gingivalis has a typical colony morphology of black, raised, glossy, smooth surface, negative Gram stain, and the bacteria are coccus or brevibacteria. Save the isolated Porphyromonas gingivalis for future use.

[0040] (2) Obtain Porphyromonas gingivalis thalline protein

[0041] Incubate the isolated Porphyromonas gingivalis in bovine brain ...

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Abstract

The invention relates to a method for preparing a yolk immunoglobulin vaccine for resisting porphyromonas gingivalis, which comprises the following operating steps of: (1) culturing and separating an international standard strain of the porphyromonas gingivalis to obtain the porphyromonas gingivalis; (2) obtaining mycoprotein of the porphyromonas gingivalis; (3) mixing a whole bacterium soluble protein antigen of the porphyromonas gingivalis with a freunds incomplete adjuvant uniformly to prepare an intravenous injection preparation, performing an intravenous injection under a chicken wing of a laying hen, and collecting eggs laid by the laying hen; and (4) extracting yolk immunoglobulin for resisting the porphyromonas gingivalis in the eggs. The method has a low production cost and a high yield because 100 milligrams of the yolk immunoglobulin can be obtained from one egg; and the method is simple, convenient and quick to operate and has the advantages of heat resistance, acid resistance, good stability and the like. Under an acid condition of which the temperature is not over 75 DEG C and the pH is more than 4, the yolk immunoglobulin vaccine can still well maintain the biological activity, can be stored for about 3 months at the normal temperature, and can be stored for 6 to 12 months at 4 DEG C with non-decreased antibody activity.

Description

technical field [0001] The invention belongs to a preparation method of a biological preparation, in particular to a preparation method of an anti-Porphyromonas gingivalis yolk immunoglobulin vaccine. Background technique [0002] Chronic periodontitis is one of the most common oral diseases, not only one of the main factors leading to human tooth loss, but also a risk factor for some systemic diseases. It has been reported that the probability of coronary heart disease and stroke in patients with periodontitis is 1.4 times and 2.1 times that of those with normal periodontitis, respectively; periodontitis will increase the colonization of respiratory pathogens in the patient's pharynx, and the probability of chronic lung infection and reduced lung function It is twice that of those with good oral hygiene; pregnant women with severe periodontitis are 7.5 times more likely to have premature births and low birth weight babies than pregnant women with normal periodontitis; the i...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61K39/02A61P31/04A61P1/02C07K16/02
Inventor 徐燕沈继龙姜大川罗庆礼
Owner ANHUI MEDICAL UNIV
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