6results about How to "Guaranteed biological activity" patented technology

The invention belongs to the field of bioengineering, and discloses a soluble humanized III-type collagen with a stable triple helix structure, and a preparation method and application thereof. According to the invention, a bioinformatics method is adopted to preferably splice functional fragments from a humanized III-type collagen sequence, and a coding gene is integrated into a pichia pastoris genome in a multi-copy form to obtain a high-expression humanized III-type collagen strain; high-expression target protein is obtained through biological fermentation, the target protein with the purity of 95% or above can be obtained through one-step chromatography, the production efficiency is obviously improved, the process is simple and easy to operate, and the method is suitable for industrial amplification. The prepared humanized III-type collagen has a triple helix structure, is stable in structure, has the activities of promoting cell migration, promoting cell proliferation, resisting inflammation and the like, and has great value in the fields of medical beauty and medicine.

The invention discloses a vibrio cantoniensis phage VCP11AVCGD4 as well as a composition and application of the vibrio cantoniensis phage VCP11AVCGD4, the vibrio cantoniensis phage VCP11AVCGD4 is preserved in Guangdong Microbiological Culture Collection Center on November 24, 2025, the preservation number of the vibrio cantoniensis phage VCP11AVCGD4 is GDMCC NO. 67343-B1, and the vibrio cantoniensis phage VCP11AVCGD4 is classified and named as Vibrio campbelli phage VCP11AVCGD4. The vibrio canbainii bacteriophage VCP11AVCGD4 provided by the invention can keep relatively high activity for a long time under the condition of 75 DEG C, is helpful to tolerate the process links of high-temperature granulation, spray drying and the like in a feed processing process and the high-temperature environment of an aquaculture water body in summer or during special treatment, and ensures the biological activity and efficacy of the vibrio canbainii bacteriophage VCP11AVCGD4 in a final product.

The present application relates to the field of biological medicine, and specifically provides an anti-LILRB4 monoclonal antibody injection preparation, which comprises an anti-LILRB4 monoclonal antibody, a buffer salt, a protein protective agent, and a surfactant, wherein the pH of the injection preparation is 5.5-6.5. The injection preparation provided by the present application provides a good storage environment for the anti-LILRB4 monoclonal antibody through the synergistic interaction of different contents of the buffer salt, the protein protective agent, and the surfactant, and guarantees the biological activity of the anti-LILRB4 monoclonal antibody. The anti-LILRB4 monoclonal antibody can effectively inhibit the combination of the LILRB4 antigen and its ligand complex, thereby blocking the interaction with the ligand ApoE complex, preventing the activation of the downstream NF-kappa B signal pathway and the release of ARG1, preventing the inhibition of T cell proliferation and the promotion of tissue infiltration, and can be used for treating cancer.

The application discloses a Candida albicans bivalent subunit vaccine fusion protein and a preparation method and application thereof, and relates to the technical field of biological medicines. The fusion protein is formed by connecting a Candida albicans Sap2 truncated fragment (SEQ ID NO: 1) and a Cht3 truncated fragment (SEQ ID NO: 2) through a flexible linker (SEQ ID NO: 3). The application further provides a nucleotide sequence encoding the fusion protein, a vaccine containing the fusion protein and a pharmaceutically acceptable adjuvant, and application of the fusion protein in preparation of a medicine or vaccine for preventing Candida albicans infection. The fusion protein is recombinantly expressed, enzymatically cut on a GST affinity chromatographic column and refined through ion exchange chromatography, and the purity can reach more than 95%. The fusion protein is significantly superior to a Sap2 and Cht3 physical mixing group, has an immune synergistic enhancement effect, and can be used for preventing Candida albicans infection.

The application discloses a kind of biological medicine efficient polypeptide flushing gel and preparation process, it is related to polypeptide flushing gel preparation technical field, first, at 20 ℃ with 550rpm stirring speed injection water is stirred, and slowly adding sodium carboxymethyl cellulose, after all is added, put into kudzu root polysaccharide, the stirring speed is promoted to 1200rpm and continues to mix 45 minutes, then reduce the speed to 300rpm, add sodium chloride and stir until dissolved, make up injection water, and adjust pH to 7.0-7.5, prepare water phase carrier, immediately cool to 2-8 ℃ for standby, then, in the independent 5 ℃ low temperature environment, freeze-dried polypeptide powder is slowly added into pre-cooled phosphate buffer according to the proportion of 1mg / mL, stirring and dissolving, prepare polypeptide mother liquor, then, polypeptide mother liquor is stirred with the above-mentioned standby water phase carrier, after mixing evenly, the final solution is filled and sealed, and finally using high-temperature steam sterilization method is handled.

This invention discloses a method for extracting plant growth-promoting beneficial active substances from edible fungi cultivation waste, comprising the following steps: 1) Raw material preparation: Drying the edible fungi cultivation waste to constant weight, pulverizing it, and collecting the powder for later use; 2) Oxidation pretreatment: Adding an appropriate amount of water to the edible fungi cultivation waste powder and stirring evenly to obtain a mixed liquid, then adding persulfate to the mixed liquid and reacting under stirring conditions to obtain a preliminary mixture; 3) Alkali extraction: Adding alkali to the preliminary mixture obtained in step 2) and reacting under stirring conditions to obtain a mixture; 4) Separation and post-treatment: Separating the mixture from step 3) into solid and liquid components, collecting the filtrate, concentrating and drying it to obtain plant growth-promoting beneficial active substances. This invention uses oxidation pretreatment and alkali extraction in synergistic extraction to obtain plant growth-promoting beneficial active substances rich in polysaccharides, humic acids, flavonoids, and other active ingredients, avoiding the damage to active substances caused by strong chemical reagents.