12results about How to "Guaranteed biological activity" patented technology

The invention discloses an extracellular vesicle and a preparation method and application thereof, and belongs to the technical field of cosmetics, and the preparation method comprises the following steps: mixing rose and a PBS buffer solution, juicing, and filtering to obtain a filtrate; adding cellulase, xylanase and beta-glucanase into the filtrate, carrying out enzymolysis, and filtering to obtain an enzymatic hydrolysate; and centrifuging the enzymatic hydrolysate, filtering, resuspending and precipitating to obtain the rose extracellular vesicles. The rose extracellular vesicles can guarantee structural integrity and biological activity, can effectively load active ingredients, and can effectively deliver active molecules in the rose extracellular vesicles to target cells through interaction with membranes of skin cells. The extracellular vesicles can be fused with skin cell membranes to enter cells, active ingredients encapsulated by the extracellular vesicles are released, and the absorption efficiency of skin is improved.

This utility model discloses a sample dispensing device for an in vitro diagnostic analyzer, including a connecting base plate, an arc-shaped plate, and a cooling plate. A hollow plate is disposed above the connecting base plate, and a horizontal drive block is slidably connected inside the hollow plate. A linkage plate is fixedly connected to the front side of the horizontal drive block, and a pointed pipette is fixedly connected to the bottom of the linkage plate. An air bladder is fixedly connected to the top of the pointed pipette. Two arc-shaped plates are disposed outside the pointed pipette, and the top of the arc-shaped plates is fixedly connected to the bottom of the linkage plate. A cooling plate is fixedly connected to the side of the arc-shaped plates away from the pointed pipette. In this utility model, while the sample is drawn in through the pointed pipette, the cooling plate is activated and provides cooling. This cooling is transferred to the contacting cooling plate, then to the connected arc-shaped plate, and finally conducted by the arc-shaped plate to the pointed pipette. This cooling process can prevent excessive cooling and effectively avoid sample inactivation, denaturation, or degradation due to high temperature, thus achieving the function of heat preservation for the sample in the pipette.

The invention belongs to the field of bioengineering, and discloses a soluble humanized III-type collagen with a stable triple helix structure, and a preparation method and application thereof. According to the invention, a bioinformatics method is adopted to preferably splice functional fragments from a humanized III-type collagen sequence, and a coding gene is integrated into a pichia pastoris genome in a multi-copy form to obtain a high-expression humanized III-type collagen strain; high-expression target protein is obtained through biological fermentation, the target protein with the purity of 95% or above can be obtained through one-step chromatography, the production efficiency is obviously improved, the process is simple and easy to operate, and the method is suitable for industrial amplification. The prepared humanized III-type collagen has a triple helix structure, is stable in structure, has the activities of promoting cell migration, promoting cell proliferation, resisting inflammation and the like, and has great value in the fields of medical beauty and medicine.

The invention discloses a vibrio cantoniensis phage VCP11AVCGD4 as well as a composition and application of the vibrio cantoniensis phage VCP11AVCGD4, the vibrio cantoniensis phage VCP11AVCGD4 is preserved in Guangdong Microbiological Culture Collection Center on November 24, 2025, the preservation number of the vibrio cantoniensis phage VCP11AVCGD4 is GDMCC NO. 67343-B1, and the vibrio cantoniensis phage VCP11AVCGD4 is classified and named as Vibrio campbelli phage VCP11AVCGD4. The vibrio canbainii bacteriophage VCP11AVCGD4 provided by the invention can keep relatively high activity for a long time under the condition of 75 DEG C, is helpful to tolerate the process links of high-temperature granulation, spray drying and the like in a feed processing process and the high-temperature environment of an aquaculture water body in summer or during special treatment, and ensures the biological activity and efficacy of the vibrio canbainii bacteriophage VCP11AVCGD4 in a final product.

The invention discloses a method for extracting plasma exosomes based on an ultracentrifugation method, which belongs to the technical field of biomedicine, and comprises the following main steps: firstly, carrying out differential centrifugation to remove visible components, cell debris and large vesicles in blood, ensuring no platelet residue, reducing the possibility that large particulate matters carry exosomes to settle, and extracting exosomes from the exosomes; the subsequent exosome recovery amount is increased; secondly, low-temperature operation is carried out in the whole process, so that the biological activity and the structure of the exosome can be better guaranteed, the degradation of exosome protein and RNA is effectively avoided, and the sample purity is high. According to the method for extracting the plasma exosomes based on the ultracentrifugation method, impurities such as cell debris and protein particles in the plasma are effectively removed by using a 0.22-micron filter for filtration, the purity of the exosomes is improved, and the method for extracting the plasma exosomes based on the ultracentrifugation method is short in separation time, high in repeatability, low in operation cost, large in sample treatment capacity, not prone to introducing personal errors and capable of being used for primary separation of a large number of samples.

The present application relates to the field of biological medicine, and specifically provides an anti-LILRB4 monoclonal antibody injection preparation, which comprises an anti-LILRB4 monoclonal antibody, a buffer salt, a protein protective agent, and a surfactant, wherein the pH of the injection preparation is 5.5-6.5. The injection preparation provided by the present application provides a good storage environment for the anti-LILRB4 monoclonal antibody through the synergistic interaction of different contents of the buffer salt, the protein protective agent, and the surfactant, and guarantees the biological activity of the anti-LILRB4 monoclonal antibody. The anti-LILRB4 monoclonal antibody can effectively inhibit the combination of the LILRB4 antigen and its ligand complex, thereby blocking the interaction with the ligand ApoE complex, preventing the activation of the downstream NF-kappa B signal pathway and the release of ARG1, preventing the inhibition of T cell proliferation and the promotion of tissue infiltration, and can be used for treating cancer.

The invention provides a carbon-based organic-inorganic compound fertilizer and a preparation method and application thereof.The carbon-based organic-inorganic compound fertilizer is prepared from, by weight, 10-15 parts of biomass charcoal, 20-25 parts of livestock manure, 3-5 parts of compound extract, 1-2 parts of compound polysaccharide, 10-15 parts of urea, 10-15 parts of ammonium sulfate, 10-15 parts of monoammonium phosphate and 10-15 parts of potassium sulfate; wherein the biomass charcoal is prepared from magnetic embedded charcoal and immobilized bacterium porous charcoal in a mass ratio of (2-4): 1. The carbon-based organic-inorganic compound fertilizer disclosed by the invention is scientific and reasonable in formula, forms a carbon-bacterium-pesticide-fertilizer integrated coupling system, and can provide multi-element efficient fertility guarantee and a healthy soil environment for plant growth, so that the growth and development of plants are remarkably promoted, the photosynthesis efficiency is improved, and the purposes of improving the quality and increasing the yield are achieved. In addition, soil structure can be obviously improved, soil porosity can be increased, hardening can be broken, permeability can be increased, and soil can be activated.

The application discloses a Candida albicans bivalent subunit vaccine fusion protein and a preparation method and application thereof, and relates to the technical field of biological medicines. The fusion protein is formed by connecting a Candida albicans Sap2 truncated fragment (SEQ ID NO: 1) and a Cht3 truncated fragment (SEQ ID NO: 2) through a flexible linker (SEQ ID NO: 3). The application further provides a nucleotide sequence encoding the fusion protein, a vaccine containing the fusion protein and a pharmaceutically acceptable adjuvant, and application of the fusion protein in preparation of a medicine or vaccine for preventing Candida albicans infection. The fusion protein is recombinantly expressed, enzymatically cut on a GST affinity chromatographic column and refined through ion exchange chromatography, and the purity can reach more than 95%. The fusion protein is significantly superior to a Sap2 and Cht3 physical mixing group, has an immune synergistic enhancement effect, and can be used for preventing Candida albicans infection.

The invention discloses a preparation method suitable for a cord blood mononuclear cell cryopreservation solution, and relates to the technical field of cord blood mononuclear cell cryopreservation solution preparation.The preparation method suitable for the cord blood mononuclear cell cryopreservation solution comprises the following steps that cord blood plasma is prepared, DMSO is added into the plasma which is detected to be qualified according to the proportion, and the cord blood plasma is obtained; uniformly mixing to obtain a cord blood mononuclear cell cryopreservation solution, wherein the cell cryopreservation solution contains a human mesenchymal stem cell serum-free culture medium, DMSO and human serum albumin; the cryopreservation liquid can improve the motility rate of cells and increase the recovery yield after the cells are unfrozen, and can effectively protect the cells and reduce the cell loss when the mononuclear cells of umbilical cord blood are recovered at low temperature by adding corresponding additives; according to the umbilical cord blood mononuclear cell cryopreservation method, under the condition that the prepared cryopreservation liquid is used, the survival rate of resuscitated cells reaches 90% or above, the biological characteristics of the cells are not changed, and the biological activity of the umbilical cord blood mononuclear cells is guaranteed.

The invention provides a histone extraction method, a kit and application, and relates to the technical field of biology. The extraction method comprises the following steps: 1) splitting a cell nucleus; (2) adding polyethylene glycol and a glucan solution, and uniformly stirring and mixing; and 3) purifying and separating to obtain the histone. According to the extraction method, the extraction purity and yield of the histone are improved under the condition that the structure of the histone is not damaged; the extraction steps of the histone are simplified, the operation complexity and the time cost of the extraction method are reduced, and the sample treatment efficiency is improved.

The application discloses a kind of biological medicine efficient polypeptide flushing gel and preparation process, it is related to polypeptide flushing gel preparation technical field, first, at 20 ℃ with 550rpm stirring speed injection water is stirred, and slowly adding sodium carboxymethyl cellulose, after all is added, put into kudzu root polysaccharide, the stirring speed is promoted to 1200rpm and continues to mix 45 minutes, then reduce the speed to 300rpm, add sodium chloride and stir until dissolved, make up injection water, and adjust pH to 7.0-7.5, prepare water phase carrier, immediately cool to 2-8 ℃ for standby, then, in the independent 5 ℃ low temperature environment, freeze-dried polypeptide powder is slowly added into pre-cooled phosphate buffer according to the proportion of 1mg / mL, stirring and dissolving, prepare polypeptide mother liquor, then, polypeptide mother liquor is stirred with the above-mentioned standby water phase carrier, after mixing evenly, the final solution is filled and sealed, and finally using high-temperature steam sterilization method is handled.

This invention discloses a method for extracting plant growth-promoting beneficial active substances from edible fungi cultivation waste, comprising the following steps: 1) Raw material preparation: Drying the edible fungi cultivation waste to constant weight, pulverizing it, and collecting the powder for later use; 2) Oxidation pretreatment: Adding an appropriate amount of water to the edible fungi cultivation waste powder and stirring evenly to obtain a mixed liquid, then adding persulfate to the mixed liquid and reacting under stirring conditions to obtain a preliminary mixture; 3) Alkali extraction: Adding alkali to the preliminary mixture obtained in step 2) and reacting under stirring conditions to obtain a mixture; 4) Separation and post-treatment: Separating the mixture from step 3) into solid and liquid components, collecting the filtrate, concentrating and drying it to obtain plant growth-promoting beneficial active substances. This invention uses oxidation pretreatment and alkali extraction in synergistic extraction to obtain plant growth-promoting beneficial active substances rich in polysaccharides, humic acids, flavonoids, and other active ingredients, avoiding the damage to active substances caused by strong chemical reagents.