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Cell freezing medium for treating leukemia

A cryopreservation liquid and cell technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of stem cell damage, cytotoxicity, etc., achieve reasonable price, simple and feasible operation, and ensure cell biological activity Effect

Active Publication Date: 2016-03-30
重庆斯德姆生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, DMSO can be toxic to cells and cause irreversible damage to cryopreserved stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation of embodiment 1 cell cryopreservation liquid

[0019] Dimethyl sulfoxide 5%w / v, 0.5%w / v LDL, 1%w / v trehalose, 1%w / v lecithin, polypeptide 1%w / v, bFGF1%w / v, vitamin E 1% w / v, and finally use DMEM medium and fetal bovine serum to adjust the volume to 100ml according to the ratio of 1:1, wherein the sequence of the polypeptide is shown in SEQ ID NO:1.

Embodiment 2

[0020] The preparation of embodiment 2 cell cryopreservation liquid

[0021] Dimethyl sulfoxide 5%w / v, 0.5%w / v LDL, 1%w / v trehalose, 1%w / v lecithin, polypeptide 1%w / v, bFGF1%w / v, Vitamin E 1% w / v, and finally use DMEM medium and fetal bovine serum to adjust the volume to 100ml according to the ratio of 1:1, wherein the sequence of the polypeptide is shown in SEQ ID NO:2.

Embodiment 3

[0022] The preparation of embodiment 3 cell cryopreservation liquid

[0023] Dimethyl sulfoxide 5%w / v, 0.5%w / v LDL, 1%w / v trehalose, 1%w / v lecithin, polypeptide 1%w / v, bFGF1%w / v, vitamin E 1% w / v, and finally use DMEM medium and fetal bovine serum to adjust the volume to 100ml according to the ratio of 1:1, wherein the sequence of the polypeptide is shown in SEQ ID NO:3.

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PUM

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Abstract

The invention provides a cell freezing medium and a preparation method thereof. The cell freezing medium is prepared from 5 percent w / v dimethyl sulfoxide, 0.5 percent w / v low density lipoprotein, 1 percent w / v trehalose, 1 percent w / v lecithin, 1 percent w / v polypeptide, 1 percent w / v bFGF (Basic Fibroblast Growth Factor), and 1 percent w / v vitamin E; finally, the volume of the cell freezing medium is fixed to 100 ml by using a DMEM culture medium and fetal calf serum in the proportion of 1:1. The cell freezing medium provided by the invention is used for freezing culture cells, and particularly, the survival rate of frozen cells after the frozen cells resuscitate is improved. The cell freezing medium has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of tissue cell culture, in particular to a cell cryopreservation solution and its preparation method and application. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells and adult stem cells according to their developmental stage. Stem cells are divided into three categories according to their developmental potential: totipotent stem cells, pluripotent stem cells, and unipotent stem cells (multipotent stem cells). Stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body, and are called "universal cells" in the medical field. In all nervous tissues such as the brain and spinal cord, different types of neural stem cells produce different ty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C07K7/08
CPCA01N1/0221C07K7/08
Inventor 潘时辉
Owner 重庆斯德姆生物技术有限公司