37 results about "Developmental stage" patented technology

The invention discloses a mouse embryo quality and development stage combined prediction system and method based on deep learning, and the system comprises a data preprocessing module which is used for obtaining a time sequence data set, and a feature extraction module which is used for processing the time sequence data set through a pre-trained OfficientNet model, and obtaining the feature vector of an embryo image corresponding to each time point; the time sequence feature fusion module is used for processing the image feature vectors of all embryos arranged according to the time sequence by using a bidirectional long-short-term memory network to obtain hidden state vectors of all embryo image fusion time sequence information; and the multi-task prediction module is used for inputting the hidden state vector into the embryo quality prediction and development stage classification prediction model after joint optimization to obtain a splicing result of embryo quality prediction and development stage classification prediction. According to the method, two prediction tasks of embryo quality and development stage classification can be processed at the same time by utilizing the combination of the OfficientNet model and the bidirectional long-short-term memory network, so that the model processing efficiency is improved.

This invention discloses an indoor breeding method for ladybugs, belonging to the field of biological control. This invention uses beet armyworms to partially replace whiteflies, the natural food source for ladybugs at different developmental stages, thereby reducing the amount of whiteflies required. Since the artificial feeding technology for beet armyworms is currently very mature, beet armyworm eggs can be obtained stably and efficiently. Therefore, this invention, by using beet armyworm eggs to partially replace whiteflies, the natural food source for ladybugs, simplifies the indoor breeding process of ladybugs, reduces breeding costs, and has good application prospects.

This invention discloses a primer pair for identifying the barbaric harvester ant and its application, relating to the field of molecular biology. The invention discloses primer pairs with nucleotide sequences as shown in SEQ ID NO:5 and SEQ ID NO:6. It overcomes the limitations of traditional morphological detection and can effectively distinguish the barbaric harvester ant from nine closely related species (*C. spp.*, *C. brevicornu*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, and *C. spp.*). It is suitable for detecting different developmental stages of the barbaric harvester ant, achieving rapid, accurate, and stable detection with a sensitivity as low as 0.01 ng / μL.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of the PoPILS1a gene in regulating plant seed development. The coding sequence of the PoPILS1a gene is shown in SEQ ID NO:1. This invention is the first to discover that PoPILS1a is specifically expressed in peony seed tissues, and the expression level varies significantly at different developmental stages. The PoPILS1a protein is located in the endoplasmic reticulum. In Arabidopsis thaliana positive lines with overexpression of PILS1a, the attachment point between pods is significantly shortened, and the number of pods distributed within a limited inflorescence length is greater. Simultaneously, the length and weight of pods at corresponding positions show significant differences compared to the control, clarifying that PILS1a can promote fruit development and has good practical application value.

We provide the system. [Solution] A means for capturing video of the baby and sending it to a server, A means for detecting the baby's behavior from the video data using an image analysis algorithm, A means for evaluating the baby's developmental stage based on the detected behavior using a machine learning model, A means for generating childcare advice based on the aforementioned evaluation results, A means for transmitting and displaying the aforementioned childcare advice on a terminal, A system that includes this.

The present invention discloses a system and a method for detecting different developmental stages of fungi life cycle in postharvest crops. More particularly, the system comprises a measuring transducer, such as a CMOS photodetector and immobilized fungal nucleic acid strands. RNA from the tested postharvest produce sample is loaded onto the system of the present invention, and specific fungal mRNA transcripts anneal to the immobilized strands. A reporter strand conjugated to a signal-generating component further binds the mRNA transcripts, and when a detectable reaction is produced post binding, the transducer captures said reaction and converts it to measurable values, indicating the quantity, presence and the developmental stage of the fungus.

This invention discloses a preprocessing method for the spatial transcriptome of maize anthers based on morphological restoration and precise permeation. The method includes: attaching and fixing maize anther tissue sections onto a spatial transcriptome capture chip; staining with a preferred concentration (1 / 200) of fluorescent whitening agent (FB28), followed by moist incubation to allow the anther exowall cells to absorb water and reshape; imaging and localization while maintaining chip surface hydration throughout the process; precise permeation treatment for 7 minutes using a permeation reagent; and finally, in situ cDNA synthesis. This invention effectively overcomes the localization challenge caused by maize anther tissue shrinkage through the synergistic effect of staining and restoration processes. Combined with optimized permeation parameters, it solves the permeation problem at different developmental stages of anthers, significantly improving the spatial resolution of sequencing data and making it suitable for spatial transcriptome studies of the entire developmental cycle of maize anthers.

This invention relates to the field of molecular biology, specifically to an internal reference gene of whorled holly and its application, and particularly to... Actin2 and / or 28S As an internal reference gene for quantitative analysis of Ilex chinensis genes, the aforementioned Actin2 The nucleotide sequence is shown in SEQ ID NO.1. 28S The nucleotide sequence is shown in SEQ ID NO.2. This invention provides an internal reference gene for analyzing the expression of target genes in samples from different tissues of *Ilex verticillata* at different developmental stages and under different hormone treatments. Actin2 and / or 28S The study also included the detection of primer pairs. This research provides technical support and reliable evidence for the study of gene expression characteristics in whorled holly and subsequent research related to flower buds.

The invention discloses a mulberry heat shock transcription factor MaHSF1 as well as a coding gene and application thereof, and aims to solve the technical problem that high-temperature-resistant gene resources and germplasm resources of mulberry are deficient at present. On the basis of the heat shock transcription factor MaHSF1 which is screened from a mulberry HSF gene family and is obviously subjected to high-temperature stress up-regulation and heterologous expression of which can improve the high-temperature resistance of the yeast, the over-expression of the heat shock transcription factor MaHSF1 can inhibit the increase of the content of malondialdehyde MDA at high temperature; the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and the content of osmotic regulation substance free proline are improved, so that the high-temperature resistance of arabidopsis thaliana is improved; the expression of the promoter has developmental stage specificity and heat stress induction characteristics, which indicates the accuracy of the regulation mechanism of the promoter. According to the application, the function and the action mechanism of the MaHSF1 and the coding gene thereof for resisting the high-temperature stress damage are verified for the first time, and a solid theoretical basis is provided for the mulberry production to cope with the high-temperature stress damage and the breeding of new high-temperature-resistant varieties.

Disclosed herein include methods and compositions for culture medias for in vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.

A Solanaceous plant exhibiting a parthenocarpy and down-regulation in activity or expression of MBP3 and at least one of AGL6 and MBP22, as compared to a control plant, wherein the control plant is of the same genetic background and developmental stage. Also provided are methods of producing the plant and uses thereof.

The invention discloses a method for constructing a crop leaf age simulation model, and the method comprises the following steps: (1) collecting the phenological data of a to-be-simulated crop at a research site, and obtaining the day order of the beginning of the development period of the to-be-simulated crop, all key development stages, and the meteorological data of the research site; (2) according to the data obtained in the step (1), continuously calculating three types of development period process simulation values of the to-be-simulated crop under a day-by-day or hour-by-hour scale through a temperature difference method, a temperature accumulation method and a physiological development time method, and obtaining leaf age simulation values under the three types of development period processes by utilizing a quantitative relation between leaf age and the three types of development period process simulation values; and (3) integrating the leaf age simulation values obtained in the step (2) under the three developmental period processes to obtain a leaf age integrated simulation value of the to-be-simulated crop. The crop leaf age simulation model constructed by the method is high in precision, and technical support is provided for intelligent production management of horticultural crops.

A lentivlral vector for real-time monitoring of cell state which includes a regulatory element that regulates expression of a selection marker, wherein the regulatory element comprises cell-state-specific histone H3K27-acetylation marks and / or chromatin accessibility. Methods of identifying a cell- state-specific regulatory element in a cell; optically screening a compound for the ability to modify cell identity; in vivo labeling normal and / or diseased cells at a specific developmental stage or a specific stage of disease; and real- time monitoring of transcription of a cell exposed to a stress using the lentiviral vector.

The invention discloses an application of kochia saponin in preparation of a medicine for treating helminthosis, an in-vitro effectiveness experiment result of the kochia saponin on an echinococcosis multilocularis protoscolex shows that the killing effect of the kochia saponin on the protoscolex has time and concentration dependence, the death rates of 10 [mu] M, 20 [mu] M and 50 [mu] M of kochia saponin treated for 6 h are 8.05% + / -0.39%, 89.23% + / -1.48% and 100% respectively, and the death rates of 10 [mu] M, 20 [mu] M and 50 [mu] M of kochia saponin treated for 24 h are all 100%; meanwhile, results of in-vitro effectiveness experiments of kochia saponin on trichina at different development stages show that after the trichina is treated for 72 hours under the concentration of 50 mu M, 80 mu M and 100 mu M, the death rates of muscular larvae are 86.24% + / -1.15%, 90.17% + / -1.24% and 90.86% + / -2.10% respectively; after treatment for 24 hours, the death rates of the newborn larvae are respectively 12.71% + / -2.36%, 57.80% + / -2.23% and 79.60% + / -1.52%, all the newborn larvae die after 48 hours, and the killing effect is obvious. Besides, the applicant confirms that the mogroside can directly destroy the epidermis structure and cell integrity of the insect body for the first time through microscope observation, and the discovery provides visual morphological evidence for the anti-parasitic effect of the mogroside.

The invention discloses an adult (final) height prediction method. The method comprises the following steps: 1) respectively evaluating the bone maturity levels of the radius and the first metacarpal bone of a subject, and calculating the average value of the bone maturity levels; the method comprises the steps of (1) selecting a bone maturity grade average value, (2) evaluating the adolescent developmental stage of a subject, (3) finding the percentage (% FHA) of the height corresponding to the developmental stage to the lifelong height from a pre-established table according to the bone maturity grade average value and the adolescent developmental stage, and (4) predicting the adult (final) height = current height / (% FHA / 100) The method is simple and convenient to operate, short in time consumption, high in prediction accuracy and convenient for clinical popularization and application.

This invention discloses a molecular marker method and its application for assisting in the identification of low-nitrogen-tolerant millet, comprising the following steps: Step 1: Extracting DNA from the millet seed to be tested; Step 2: Performing PCR amplification on the millet DNA; Step 3: Detecting the PCR amplification product. If a specific fragment of 501 bp is amplified, it is a low-nitrogen-tolerant millet variety. Experiments have demonstrated that SiM65737 of this invention is a molecular marker related to low-nitrogen tolerance in millet, which can be used for early screening and prediction of nitrogen utilization in millet, and can be used for marker-assisted breeding and the selection of low-nitrogen-tolerant millet varieties. The SiM65737 molecular marker can be detected in different tissues and developmental stages of millet, which facilitates faster screening and detection of nitrogen absorption and utilization efficiency in millet.

Disclosed herein include methods and compositions for culture medias for in vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.

The application discloses a kind of based on dynamic phase line chart cross-age developmental stage monitoring method and device, comprising: the relationship model between the known developmental stage of different ages of the person to be monitored is constructed;The age of each to-be-monitored interval of the person to be monitored is input into the relationship model of each developmental stage, the corresponding output value is obtained by prediction and is mapped as the numerical value of the age of each to-be-monitored interval in the normalized probability mass function corresponding to each developmental stage;The midpoint cumulative probability corresponding to the age of each to-be-monitored interval in each developmental stage is calculated using the intermediate P value method, and the midpoint cumulative probability is converted into the corresponding standard deviation score using the probit function;According to the standard deviation score corresponding to the age of each to-be-monitored interval in each developmental stage, a dynamic phase line chart is drawn, the evolution trend of developmental stage over time is visualized through the dynamic phase line chart, and the abnormal development period is identified.The application can reduce subjective misjudgment of developmental stage abnormalities.

This invention discloses a crayfish egg counting, screening, and hatching device based on image recognition, belonging to the field of crayfish breeding technology. It aims to solve the technical problems of current traditional artificial crayfish hatching facilities, including numerous drawbacks such as cumbersome container installation and disassembly, low hatching efficiency, low level of intelligence, and limited water treatment methods, insufficient oxygenation, and untimely cleaning of mold and debris, leading to water pollution, low hatching survival rates, and increased production risks and costs. The invention includes a screening mechanism and a hatching mechanism. This invention provides three different drainage methods, allowing for diverse selection. Farmers can flexibly adjust the drainage method according to actual needs, such as the developmental stage of the crayfish eggs, water quality, and environmental conditions, to better meet the various needs of crayfish egg hatching. This enables precise control and management of the hatching process, ensuring stable water quality while avoiding impact on the embryos, effectively improving the hatching success rate.

The invention discloses a manufacturing method of a cinnamon vascular tissue paraffin section. According to the method, a self-adaptive gradient heating wax dipping technology is adopted, and the texture difference of cinnamon materials in different development stages from tender shoots to semi-lignified stems can be adapted. According to the method, the traditional chemical softening step is abandoned, the authenticity of the original components and the microstructure of the vascular tissue is completely reserved, and the slide preparation period is remarkably shortened. The paraffin section prepared by the method is high in tissue and cell morphology integrity, high in dyeing contrast and real. In addition, the method is reasonable in process design, effectively reduces dependence on expensive equipment, has universality and high efficiency, and solves the problems that the cell structure is damaged due to dependence on chemical softening and the requirements of soft and hard material slices are difficult to consider in the traditional method.

The invention discloses specific primers and a method for rapidly identifying leaf miners, and belongs to the technical field of molecular biology. According to the method, rapid identification of leaf miners is realized mainly by designing specific primers. The primer disclosed by the invention has high specificity, only performs specific amplification on the COI gene of the leaf miner, and does not have an amplification product on sibling species such as Lithocolletis ringoniella, Grapholitha molesta, Ampelopsis molesta, codling moth and Phytophorus nigricans. The primer is suitable for detecting different development stages (first-instar larvae, second-instar larvae, third-instar larvae, pupae and adults) of leaf miners and different tissue parts (tentacles, head chest, abdomen, wings and feet) of the adults, the annealing temperature range is wide, and the sensitivity is high. The method is easy and convenient to operate, the detection time is short (2-3 hours), the method can be widely applied to port quarantine, field monitoring and other scenes, and technical support is provided for precise prevention and control of leaf miners.

This invention relates to the fields of molecular biology and genetic engineering, specifically to the application of nematode nicotinamide genes in regulating the resistance of soybean cyst nematode infection. Three candidate nicotinamide genes from soybean cyst nematodes were screened through structural alignment. Hetgly07149, Hetgly05004, Hetgly05016 The study found that the nicotinamide gene plays a positive regulatory role in nematode infection of plants. After silencing this gene using host-induced gene silencing (HIGS) technology, the infection ability of soybean cyst nematodes in both the susceptible variety Wm82 and the resistant variety Forrest soybean was significantly reduced. The proportion of nematodes in late developmental stages (J3 and J4) and the total number of nematodes invading the host plant were also significantly decreased. This provides an important target gene and theoretical basis for developing novel transgenic crops resistant to soybean cyst nematodes using HIGS technology, which is of great significance for protecting food security for crops such as soybeans.

The invention relates to a method for isolating embryonic stem cells (100) from at least one avian embryonic cell, comprising: isolating at least one embryo (101) around the developmental stage of oviposition; suspending embryonic cells (102) obtained from the embryo by dissociation step a) in an animal serum-free base medium supplemented with a combination of growth factors, at least one inhibitor and an animal serum substitute; seeding the embryonic cell suspension (103) on a feeder cell layer; culturing (104) the embryonic cells at least once passaged.

This invention relates to the field of image recognition technology, specifically to a method and system for blueberry fruit detection based on image recognition. The method involves imaging and monitoring blueberry fruits using cross-polarization imaging technology to obtain polarized images and standard images of blueberries. The standard image is used to identify the developmental stage of the blueberry. Based on the developmental stage, an image segmentation strategy is selected to adaptively segment the polarized image, obtaining a bloom mask. The polarized image, standard image, and bloom mask are identified to obtain bloom features. Time-stamped bloom features are acquired to obtain bloom temporal features. The evolution of bloom features and the synergistic relationship between the bloom features and the fruit peel background color are analyzed and compared with standard temporal features to classify the fruit into normal and abnormal development types. This invention outputs fruit detection information through the identification of bloom temporal features of normal and abnormal development types.

The invention discloses an application of psoralen B in preparation of a medicine for treating helminthosis, an in-vitro effectiveness experiment result of psoralen B on an echinococcosis multilocularis protoscolex shows that the killing effect of maduramicin ammonium on the protoscolex has concentration dependence, the death rate of the protoscolex can reach 90.63% + / -0.96% after the maduramicin ammonium is treated for 6 h at the concentration of 50 mu M, and the death rate of the protoscolex can reach 90.63% + / -0.96% at the concentration of 100 mu M, and the death rate of the maduramicin ammonium can reach 90.63% + / -0.96% at the concentration of 100 mu M. The death rate of the original head section can directly reach 100%; meanwhile, in-vitro effectiveness experiment results of psoralen B on trichina at different development stages show that after the trichina is treated for 72 hours under the concentration of 50 mu M, 80 mu M and 100 mu M, the death rates of muscular larvae reach 89.44% + / -0.81%, 96.29% + / -1.30% and 96.68% + / -1.06% respectively; after 48 hours of treatment, the death rate of the adults is 100%, and the killing effect is remarkable. More importantly, the applicant verifies that IBC can directly destroy the epidermal structure and cell integrity of the insect body for the first time through observation by an optical microscope and an electron microscope, and the discovery provides intuitive morphological evidence for the anti-parasitic effect of IBC.

The invention belongs to but is not limited to the technical field of gene engineering, and particularly relates to a preparation method and application of unlabeled sebastes schlegeli IGF2 recombinant protein, and by detecting the IGF2 expression level in different development stages of ovary, it is found that IGF2 has an important regulation effect on fertilized ovary matrix and embryo. The cDNA of sebastes schlegeli is used as a template for PCR amplification to obtain the sebastes schlegeli IGF2 mature peptide, and the amino acid sequence of the sebastes schlegeli IGF2 mature peptide is as shown in SEQ ID NO: 2. The recombinant protein comprises the IGF2 mature peptide, a His-Sumo tag connected with the N end of the IGF2 mature peptide, and a connecting peptide fragment between the His tag and the Sumo tag, and the amino acid sequence is shown as SEQ ID NO: 1. The invention also provides application of the sebastes schlegeli IGF2 mature peptide recombinant protein in promoting proliferation of primary ovarian cells and development of germ cells of sebastes schlegeli.

This invention provides a method for assessing embryonic development quality based on a Smart Memory Network (SMMC). The method involves inputting embryonic images at different developmental stages; assigning different weights to the embryonic images according to the developmental stage, and outputting an attention feature map with channel attention; extracting global and local features from the attention feature map; fusing the global and local features and extracting temporal features to obtain a spatiotemporal feature map; and inputting the spatiotemporal feature map into a binary classification fully connected layer for classification to assess embryonic development quality. By extracting features from various aspects, the method of this invention exhibits superior performance in embryo assessment.

This invention belongs to the field of molecular biology and relates to animal genetics and breeding. It specifically provides genes, primers, applications, and methods for identifying the developmental stages of the clitellum in *Pheretima aspergillum*. Addressing the shortcomings of existing artificial breeding methods for *Pheretima aspergillum*, which rely heavily on visual observation of the clitellum and suffer from significant physiological lag, this invention is the first to discover and screen specific molecular marker genes closely related to the initiation and maturity stages of clitellum differentiation in *Pheretima aspergillum*, and provides highly specific amplification primer sets and detection methods. This invention achieves very early molecular early warning and objective quantitative identification of clitellum development in *Pheretima aspergillum*, providing molecular standards for the quantitative control of nutritional intervention and mating / harvesting timing in artificial breeding. This technology helps to break through the bottleneck of large-scale *Pheretima aspergillum* farming, reduce dependence on wild resources, and is of great significance for protecting ecological red lines and the safety of the traditional Chinese medicine industry.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to ovary maturation of freshwater shrimps, a detection primer and application of the SNP marker and the detection primer, and belongs to the technical field of biology. Aiming at the problem of breeding of late-maturing varieties of freshwater shrimps, the SNP marker provided by the invention is located in an exon region of a cathepsin D gene of the freshwater shrimps and is specifically located at the 15207bp position of the 5'end of a cathepsin D gene sequence, and a basic group at the position is C or T; the gonad development index of a CC genotype individual is obviously smaller than that of a TT genotype individual and a CT genotype individual (Plt; 0. 05), and the site does not change the composition of amino acid. The SNP marker provided by the invention not only can rapidly and stably identify the female shrimps with slow ovarian development, but also can be not limited by different development stages of the female freshwater shrimps, and can be used for early breeding of the freshwater shrimps, so that the parent screening efficiency is improved, and the breeding period is shortened.

The present invention relates to a method for plant genome modification of at least one plant cell being in the developmental stage of a plant immature inflorescence meristem (IIM) cell, wherein the modification of the specific cell type is achieved by providing a genome modification or editing system, optionally together with at least one regeneration booster, preferably wherein the effector molecules are introduced by means of particle bombardment. To this end, new artificial and precisely controllable booster genes and proteins are provided. Further, the modified plant cells are regenerated in a direct or an indirect way. Finally, methods, tools, constructs and strategies are provided to effectively modify at least one genomic target site in a plant cell, to obtain said modified cell and to regenerate a, plant tissue, organ, plant or seed from such modified cell.