65 results about "Developmental stage" patented technology

The invention discloses application of a ZaSPL7 gene in regulation and control of hairy bodies and parthenocarpy of plants, and belongs to the technical field of gene engineering. The gene ZaSPL7 for regulating and controlling organ development of the green prickleyash is separated from cDNA of the green prickleyash and is overexpressed in a plant, the number and density of epidermal hair of the transgenic plant are obviously increased, meanwhile, the number of fruit seeds is obviously reduced, even part of plants have seedless fruit phenotypes, and the unisexual setting rate is increased. The results show that the green prickleyash SPL7 gene may coordinate epidermal hair and fruit development by integrating development stage signals, and has important application value in regulation and control of epidermal appendages (such as thorns and hair) formation and apomixis, and the mechanism provides a new idea for simultaneous improvement of plant stress resistance (by reducing the thorns) and fruit commodity (by seedless). Important theoretical basis and technical support are provided for breeding of the green prickleyash, and the method has a wide application prospect.

The invention discloses a mouse embryo quality and development stage combined prediction system and method based on deep learning, and the system comprises a data preprocessing module which is used for obtaining a time sequence data set, and a feature extraction module which is used for processing the time sequence data set through a pre-trained OfficientNet model, and obtaining the feature vector of an embryo image corresponding to each time point; the time sequence feature fusion module is used for processing the image feature vectors of all embryos arranged according to the time sequence by using a bidirectional long-short-term memory network to obtain hidden state vectors of all embryo image fusion time sequence information; and the multi-task prediction module is used for inputting the hidden state vector into the embryo quality prediction and development stage classification prediction model after joint optimization to obtain a splicing result of embryo quality prediction and development stage classification prediction. According to the method, two prediction tasks of embryo quality and development stage classification can be processed at the same time by utilizing the combination of the OfficientNet model and the bidirectional long-short-term memory network, so that the model processing efficiency is improved.

The present invention relates to a method for plant genome modification of at least one plant cell being in the developmental stage of a plant immature inflorescence meristem (IIM) cell, wherein the modification of the specific cell type is achieved by providing a genome modification or editing system, optionally together with at least one regeneration booster, preferably wherein the effector molecules are introduced by particle bombardment. To this end, new artificial and precisely controllable booster genes and proteins are provided. Further, the modified plant cells are regenerated in a direct or an indirect way. Finally, methods, tools, constructs and strategies are provided to effectively modify at least one genomic target site in a plant cell, to obtain the modified plant cell and to regenerate a plant tissue, organ, plant or seed from the modified plant cell.

The invention discloses a novel codon optimization method based on precise transcriptome sequencing and analysis thereof, and belongs to the technical field of bioinformatics. In order to improve the expression quantity of a target gene, corresponding accurate transcriptome data is obtained according to the development period of transgenic expression and parts such as tissues and organs, and efficient expression genes are screened to serve as a host genome expression library; the expression abundance of the gene is comprehensively considered when the use frequency of the codon of the host genome is determined, and the gene optimized by the novel codon optimization method increases the stability of the insecticidal protein gene RNA and improves the expression level of the protein. The invention provides better reference for gene modification and transformation, and provides a novel codon optimization method for high-efficiency expression of transgenes in plants.

The present invention discloses a molecular marker, primer and application thereof for identifying the color of Actinidia arguta fruit. The molecular marker of the present invention is located at 495bp upstream of the start codon of the AaLDOX gene promoter region of Actinidia arguta, which is a 29bp Indel variation. Utilizing the molecular marker and method of the present invention, the fruit color can be quickly and accurately identified in the childhood stage of Actinidia arguta by conventional molecular test detection, and the red and green Actinidia arguta germplasm resources can be efficiently distinguished. The method can be used for parent selection and seedling identification of offspring, greatly improving the efficiency and accuracy of selective breeding. The method of the present invention can be detected in each tissue, organ and each developmental stage of the plant, and has the advantages of being convenient and fast, having good specificity, high accuracy, and being unaffected by climate and environment. It can be applied to the precise, directional and efficient breeding of red Actinidia arguta, and has a large application value.

Provided is a method for obtaining the germ cell lineage of an oviparous vertebrate more efficiently than conventional techniques. A host oviparous vertebrate is prepared at a developmental stage after the development of black pigmentary cells in the retina and before the formation of multiple layers of germ cells in the genitals, and isolated undifferentiated germ cells from a donor oviparous vertebrate are transplanted into the host oviparous vertebrate.

This invention discloses an indoor breeding method for ladybugs, belonging to the field of biological control. This invention uses beet armyworms to partially replace whiteflies, the natural food source for ladybugs at different developmental stages, thereby reducing the amount of whiteflies required. Since the artificial feeding technology for beet armyworms is currently very mature, beet armyworm eggs can be obtained stably and efficiently. Therefore, this invention, by using beet armyworm eggs to partially replace whiteflies, the natural food source for ladybugs, simplifies the indoor breeding process of ladybugs, reduces breeding costs, and has good application prospects.

This invention discloses a primer pair for identifying the barbaric harvester ant and its application, relating to the field of molecular biology. The invention discloses primer pairs with nucleotide sequences as shown in SEQ ID NO:5 and SEQ ID NO:6. It overcomes the limitations of traditional morphological detection and can effectively distinguish the barbaric harvester ant from nine closely related species (*C. spp.*, *C. brevicornu*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, and *C. spp.*). It is suitable for detecting different developmental stages of the barbaric harvester ant, achieving rapid, accurate, and stable detection with a sensitivity as low as 0.01 ng / μL.

The invention discloses a method for identifying a pluripotent state of a cattle epidermal layer, and achieves the technical effect of identifying the pluripotent state of a cattle embryo, especially the pluripotent state of the cattle epidermal layer. The invention specifically discloses gene markers for different pluripotent stages and different developmental stages, and the pluripotent and developmental stages of to-be-detected bovine embryonic cells or cell clusters can be rapidly identified by using the gene markers for different pluripotent stages and different developmental stages of bovine embryos.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of the PoPILS1a gene in regulating plant seed development. The coding sequence of the PoPILS1a gene is shown in SEQ ID NO:1. This invention is the first to discover that PoPILS1a is specifically expressed in peony seed tissues, and the expression level varies significantly at different developmental stages. The PoPILS1a protein is located in the endoplasmic reticulum. In Arabidopsis thaliana positive lines with overexpression of PILS1a, the attachment point between pods is significantly shortened, and the number of pods distributed within a limited inflorescence length is greater. Simultaneously, the length and weight of pods at corresponding positions show significant differences compared to the control, clarifying that PILS1a can promote fruit development and has good practical application value.

We provide the system. [Solution] A means for capturing video of the baby and sending it to a server, A means for detecting the baby's behavior from the video data using an image analysis algorithm, A means for evaluating the baby's developmental stage based on the detected behavior using a machine learning model, A means for generating childcare advice based on the aforementioned evaluation results, A means for transmitting and displaying the aforementioned childcare advice on a terminal, A system that includes this.

The present invention discloses a system and a method for detecting different developmental stages of fungi life cycle in postharvest crops. More particularly, the system comprises a measuring transducer, such as a CMOS photodetector and immobilized fungal nucleic acid strands. RNA from the tested postharvest produce sample is loaded onto the system of the present invention, and specific fungal mRNA transcripts anneal to the immobilized strands. A reporter strand conjugated to a signal-generating component further binds the mRNA transcripts, and when a detectable reaction is produced post binding, the transducer captures said reaction and converts it to measurable values, indicating the quantity, presence and the developmental stage of the fungus.

This invention discloses a preprocessing method for the spatial transcriptome of maize anthers based on morphological restoration and precise permeation. The method includes: attaching and fixing maize anther tissue sections onto a spatial transcriptome capture chip; staining with a preferred concentration (1 / 200) of fluorescent whitening agent (FB28), followed by moist incubation to allow the anther exowall cells to absorb water and reshape; imaging and localization while maintaining chip surface hydration throughout the process; precise permeation treatment for 7 minutes using a permeation reagent; and finally, in situ cDNA synthesis. This invention effectively overcomes the localization challenge caused by maize anther tissue shrinkage through the synergistic effect of staining and restoration processes. Combined with optimized permeation parameters, it solves the permeation problem at different developmental stages of anthers, significantly improving the spatial resolution of sequencing data and making it suitable for spatial transcriptome studies of the entire developmental cycle of maize anthers.

This invention relates to the field of molecular biology, specifically to an internal reference gene of whorled holly and its application, and particularly to... Actin2 and / or 28S As an internal reference gene for quantitative analysis of Ilex chinensis genes, the aforementioned Actin2 The nucleotide sequence is shown in SEQ ID NO.1. 28S The nucleotide sequence is shown in SEQ ID NO.2. This invention provides an internal reference gene for analyzing the expression of target genes in samples from different tissues of *Ilex verticillata* at different developmental stages and under different hormone treatments. Actin2 and / or 28S The study also included the detection of primer pairs. This research provides technical support and reliable evidence for the study of gene expression characteristics in whorled holly and subsequent research related to flower buds.

The invention discloses a mulberry heat shock transcription factor MaHSF1 as well as a coding gene and application thereof, and aims to solve the technical problem that high-temperature-resistant gene resources and germplasm resources of mulberry are deficient at present. On the basis of the heat shock transcription factor MaHSF1 which is screened from a mulberry HSF gene family and is obviously subjected to high-temperature stress up-regulation and heterologous expression of which can improve the high-temperature resistance of the yeast, the over-expression of the heat shock transcription factor MaHSF1 can inhibit the increase of the content of malondialdehyde MDA at high temperature; the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and the content of osmotic regulation substance free proline are improved, so that the high-temperature resistance of arabidopsis thaliana is improved; the expression of the promoter has developmental stage specificity and heat stress induction characteristics, which indicates the accuracy of the regulation mechanism of the promoter. According to the application, the function and the action mechanism of the MaHSF1 and the coding gene thereof for resisting the high-temperature stress damage are verified for the first time, and a solid theoretical basis is provided for the mulberry production to cope with the high-temperature stress damage and the breeding of new high-temperature-resistant varieties.

Disclosed herein include methods and compositions for culture medias for in vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.

A Solanaceous plant exhibiting a parthenocarpy and down-regulation in activity or expression of MBP3 and at least one of AGL6 and MBP22, as compared to a control plant, wherein the control plant is of the same genetic background and developmental stage. Also provided are methods of producing the plant and uses thereof.

The invention discloses a method for constructing a crop leaf age simulation model, and the method comprises the following steps: (1) collecting the phenological data of a to-be-simulated crop at a research site, and obtaining the day order of the beginning of the development period of the to-be-simulated crop, all key development stages, and the meteorological data of the research site; (2) according to the data obtained in the step (1), continuously calculating three types of development period process simulation values of the to-be-simulated crop under a day-by-day or hour-by-hour scale through a temperature difference method, a temperature accumulation method and a physiological development time method, and obtaining leaf age simulation values under the three types of development period processes by utilizing a quantitative relation between leaf age and the three types of development period process simulation values; and (3) integrating the leaf age simulation values obtained in the step (2) under the three developmental period processes to obtain a leaf age integrated simulation value of the to-be-simulated crop. The crop leaf age simulation model constructed by the method is high in precision, and technical support is provided for intelligent production management of horticultural crops.

A lentivlral vector for real-time monitoring of cell state which includes a regulatory element that regulates expression of a selection marker, wherein the regulatory element comprises cell-state-specific histone H3K27-acetylation marks and / or chromatin accessibility. Methods of identifying a cell- state-specific regulatory element in a cell; optically screening a compound for the ability to modify cell identity; in vivo labeling normal and / or diseased cells at a specific developmental stage or a specific stage of disease; and real- time monitoring of transcription of a cell exposed to a stress using the lentiviral vector.

This invention belongs to the field of fish ovarian development technology and discloses a method for determining the dominant rate-limiting point of fish ovarian maturation. The method involves selecting fish ovaries at different developmental stages as materials for histological sectioning, statistically obtaining the proportion of different types of follicles during ovarian development, and, based on the proportion of different types of follicles, using spline interpolation and linear fitting data-driven modeling techniques to analyze the proportion curves, growth rates, and initial synchronous state characteristics of different types of follicles, ultimately determining the key rate-limiting point of fish ovarian development. Based on this method, the dominant rate-limiting point of fish ovarian maturation can be accurately determined, and measures can then be formulated to regulate the fish ovarian development process based on this dominant rate-limiting point. This invention also discloses the application of the method for determining the dominant rate-limiting point of fish ovarian maturation in regulating the fish ovarian development process and methods for promoting the fish ovarian maturation process.

The invention discloses an application of kochia saponin in preparation of a medicine for treating helminthosis, an in-vitro effectiveness experiment result of the kochia saponin on an echinococcosis multilocularis protoscolex shows that the killing effect of the kochia saponin on the protoscolex has time and concentration dependence, the death rates of 10 [mu] M, 20 [mu] M and 50 [mu] M of kochia saponin treated for 6 h are 8.05% + / -0.39%, 89.23% + / -1.48% and 100% respectively, and the death rates of 10 [mu] M, 20 [mu] M and 50 [mu] M of kochia saponin treated for 24 h are all 100%; meanwhile, results of in-vitro effectiveness experiments of kochia saponin on trichina at different development stages show that after the trichina is treated for 72 hours under the concentration of 50 mu M, 80 mu M and 100 mu M, the death rates of muscular larvae are 86.24% + / -1.15%, 90.17% + / -1.24% and 90.86% + / -2.10% respectively; after treatment for 24 hours, the death rates of the newborn larvae are respectively 12.71% + / -2.36%, 57.80% + / -2.23% and 79.60% + / -1.52%, all the newborn larvae die after 48 hours, and the killing effect is obvious. Besides, the applicant confirms that the mogroside can directly destroy the epidermis structure and cell integrity of the insect body for the first time through microscope observation, and the discovery provides visual morphological evidence for the anti-parasitic effect of the mogroside.

This invention provides a method, apparatus, electronic device, and readable storage medium for detecting pre-disease states, relating to the field of analytical detection. The method includes: acquiring dynamic network biomarkers related to the disease state of a target disease; the dynamic network biomarkers include the size of the regulatory network between transcription factors and messenger RNA in each disease state, where the disease state represents the developmental stage of the target disease; acquiring effective information values ​​of the dynamic network biomarkers corresponding to each disease state; and determining whether the disease state is a pre-disease state of the target disease based on the effective information values. By utilizing samples from other states to construct a gene regulatory network and using effective information values ​​from causal emergence theory as the standard for judging whether a disease state is a pre-disease state, a more stable and accurate identification of pre-disease states is achieved.

The present invention provides a targeted long noncoding double-stranded RNA, the nucleotide sequence of the sense strand of which is shown in SEQ ID NO: 1. This double-stranded RNA can inhibit the growth and development of schistosomes, reduce egg production, and effectively alleviate pathological damage to the host liver. The present invention also provides the use of this double-stranded RNA in the preparation of an anti-schistosomal drug. Animal experiments have shown that this double-stranded RNA has a significant inhibitory effect on the developmental stages of schistosomes, effectively inhibiting worm growth and development and female egg production, while also alleviating pathological damage to the host, demonstrating its significant application value and promising prospects.

The invention discloses an adult (final) height prediction method. The method comprises the following steps: 1) respectively evaluating the bone maturity levels of the radius and the first metacarpal bone of a subject, and calculating the average value of the bone maturity levels; the method comprises the steps of (1) selecting a bone maturity grade average value, (2) evaluating the adolescent developmental stage of a subject, (3) finding the percentage (% FHA) of the height corresponding to the developmental stage to the lifelong height from a pre-established table according to the bone maturity grade average value and the adolescent developmental stage, and (4) predicting the adult (final) height = current height / (% FHA / 100) The method is simple and convenient to operate, short in time consumption, high in prediction accuracy and convenient for clinical popularization and application.

This invention discloses a molecular marker method and its application for assisting in the identification of low-nitrogen-tolerant millet, comprising the following steps: Step 1: Extracting DNA from the millet seed to be tested; Step 2: Performing PCR amplification on the millet DNA; Step 3: Detecting the PCR amplification product. If a specific fragment of 501 bp is amplified, it is a low-nitrogen-tolerant millet variety. Experiments have demonstrated that SiM65737 of this invention is a molecular marker related to low-nitrogen tolerance in millet, which can be used for early screening and prediction of nitrogen utilization in millet, and can be used for marker-assisted breeding and the selection of low-nitrogen-tolerant millet varieties. The SiM65737 molecular marker can be detected in different tissues and developmental stages of millet, which facilitates faster screening and detection of nitrogen absorption and utilization efficiency in millet.

The present invention discloses the use of lncRNA IFA to regulate the expression of ACTG1 in porcine ovarian granulosa cells. The present invention predicts a sequence complementary to ACTG1 mRNA within the lncRNA IFA sequence and detects the binding of lncRNA IFA to ACTG1 mRNA through pull-down and experimental testing. Quantitative polymerase chain reaction (qPCR) is used to detect the expression levels of lncRNA IFA and ACTG1 in granulosa cells of follicles at different developmental stages. The expression level of ACTG1 is then tested after overexpression and interference with lncRNA IFA, revealing that lncRNA IFA can promote ACTG1 expression. Furthermore, CCK8, EdU, and flow cytometry analysis revealed that ACTG1 can promote proliferation and inhibit apoptosis of ovarian granulosa cells, enhance cell activity, and accelerate cell cycle progression in ovarian granulosa cells after overexpression and interference with ACTG1. This provides valuable information for research into the expression and regulation mechanisms of ACTG1.

The invention discloses an InDel marker for identifying red pulp characters of pears, a primer and application of the InDel marker. The InDel marker is located at the 4851722-4852014 bp position of a chromosome 5 of a red fragrant pear genome, and the overall length of the InDel marker is 292 bp. By utilizing the InDel marker and the method, the pulp color of the hybrid generation of the hybrid combination of the red-peel red-pulp parent and the green-peel white-pulp parent can be quickly and accurately identified in the pear seedling stage through conventional molecular detection, and the green-peel white-pulp pears and the hybrid generation of the red-peel red-pulp pears are efficiently distinguished. The method disclosed by the invention can be used for detecting each tissue, organ and each development stage of the plant, has the advantages of convenience, rapidness, good specificity, high accuracy, no influence of climate environment and the like, can be used for early auxiliary selection of the phenotype of the red flesh of the pear plant, can greatly improve the breeding selection efficiency and accuracy of the red flesh pear variety, and has a wide application prospect. The blank in the field of pear plant red pulp character molecular marker development is filled up, and great promotion significance is achieved for pear germplasm improvement and fruit value improvement.

Disclosed herein include methods and compositions for culture medias for in vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.

The application discloses a kind of based on dynamic phase line chart cross-age developmental stage monitoring method and device, comprising: the relationship model between the known developmental stage of different ages of the person to be monitored is constructed;The age of each to-be-monitored interval of the person to be monitored is input into the relationship model of each developmental stage, the corresponding output value is obtained by prediction and is mapped as the numerical value of the age of each to-be-monitored interval in the normalized probability mass function corresponding to each developmental stage;The midpoint cumulative probability corresponding to the age of each to-be-monitored interval in each developmental stage is calculated using the intermediate P value method, and the midpoint cumulative probability is converted into the corresponding standard deviation score using the probit function;According to the standard deviation score corresponding to the age of each to-be-monitored interval in each developmental stage, a dynamic phase line chart is drawn, the evolution trend of developmental stage over time is visualized through the dynamic phase line chart, and the abnormal development period is identified.The application can reduce subjective misjudgment of developmental stage abnormalities.

This invention discloses a crayfish egg counting, screening, and hatching device based on image recognition, belonging to the field of crayfish breeding technology. It aims to solve the technical problems of current traditional artificial crayfish hatching facilities, including numerous drawbacks such as cumbersome container installation and disassembly, low hatching efficiency, low level of intelligence, and limited water treatment methods, insufficient oxygenation, and untimely cleaning of mold and debris, leading to water pollution, low hatching survival rates, and increased production risks and costs. The invention includes a screening mechanism and a hatching mechanism. This invention provides three different drainage methods, allowing for diverse selection. Farmers can flexibly adjust the drainage method according to actual needs, such as the developmental stage of the crayfish eggs, water quality, and environmental conditions, to better meet the various needs of crayfish egg hatching. This enables precise control and management of the hatching process, ensuring stable water quality while avoiding impact on the embryos, effectively improving the hatching success rate.