262 results about "Developmental stage" patented technology

Gene expression profiling reveals four distinct subgroups of multiple myeloma that have significant correlation with various clinical characteristics. Diagnosis for multiple myeloma (and possibly monoclonal gammopathy of undetermined significance) based on differential expression of 14 genes, as well as prognosis for the four subgroups of multiple myeloma based on the expression of 24 genes are established. A 15-gene model that classifies myeloma into 7 groups is also reported. Gene expression profiling also allows placing multiple myeloma into a developmental schema parallel to that of normal plasma cell differentiation. Development of a gene expression- or developmental stage-based classification system for multiple myeloma would lead to rational design of more accurate and sensitive diagnostics, prognostics and tumor-specific therapies for multiple myeloma.

Provided herein are methods for diagnosing and treating multiple myeloma based on statistical analysis of and subsequent increasing / inhibiting expression of subgroups of plasma cells and B cell genes. Also provided are methods for a developmental stage-based classification for multiple myeloma using hierarchical clustering analysis of plasma cell and B cell nucleic acids and for discriminating among normal, hyperplastic and malignant using gene expression array data and statistical analysis thereof. In addition methods for determining the risk of developing bone disease in a test individual by examining expression levels of a WNT signaling antagonist, such as DKK1, are provided. A kit comprising anti-DKK1 antibodies and detection reagents for measuring DKK1 protein levels also is provided.

A method and device are provided for counting cells in a sample of living tissue, such as an embryo. The method involves obtaining a microscopic image of the unstained tissue that reveals cell boundaries, such as a differential interference contrast (DIC) image, and an optical quadrature microscopy (OQM) image which is used to prepare an image of optical path length deviation (OPD) across the cell cluster. The boundaries of individual cells in the cell cluster are modeled as ellipses and used, together with the maximum optical path length deviation of a cell, to calculate ellipsoidal model cells that are subtracted from the OPD image. The process is repeated until the OPD image is depleted of phase signal attributable to cells of the cell cluster, and the cell count is obtained from the number of cells subtracted. The method is capable of accurately and non-invasively counting the number of cells in a living embryo at the 2-30 cell stage, and can be employed to assess the developmental stage and health of human embryos for fertility treatments.

Methods and compositions are provided for reducing the level of expression of a target polynucleotide in an organism. The methods and compositions selectively silence the target polynucleotide through the expression of a chimeric polynucleotide comprising the target for a sRNA (the trigger sequence) operably linked to a sequence corresponding to all or part of the gene or genes to be silenced. In this manner, the final target of silencing is an endogenous gene in the organism in which the chimeric polynucleotide is expressed. In a further embodiment, the miRNA target is that of a heterologous miRNA or siRNA, the latter of which is coexpressed in the cells at the appropriate developmental stage to provide silencing of the final target when and where desired. In a further embodiment, the final target may be a gene in a second organism, such as a plant pest, that feeds upon the organism containing the chimeric gene or genes. Compositions further comprise vectors, seeds, grain, cells, and organisms, including plants and plant cells, comprising the chimeric polynucleotide of the invention.

An array of multi-stage configured cleansing wipe products may include first stage cleansing wipes and second stage cleansing wipes. The first stage cleansing wipes may have a first structure and a first composition selected in view of a first child developmental stage. The second stage cleansing wipes may have a second structure and a second composition selected in view of a second child developmental stage. The first structure and the first composition are different and potentially progressive from first stage to second stage. First stage product packaging may include at least one first stage-specific indicia and second stage product packaging may include at least one second stage-specific indicia. Stages of the array may be communicated and tailored based upon the foods eaten and the manner of eating (such as being fed or self-feeding) of the babies or children in each stage.

An array of multi-stage configured cleansing wipe products may include first stage cleansing wipes and second stage cleansing wipes. The first stage cleansing wipes may have a first structure and a first composition selected in view of a first child developmental stage. The second stage cleansing wipes may have a second structure and a second composition selected in view of a second child developmental stage. The first structure and the first composition are different and potentially progressive from first stage to second stage. First stage product packaging may include at least one first stage-specific indicia and second stage product packaging may include at least one second stage-specific indicia. Stages of the array may be communicated and tailored based upon the foods eaten and the manner of eating (such as being fed or self-feeding) of the babies or children in each stage.

A method and device are provided for counting cells in a sample of living tissue, such as an embryo. The method involves obtaining a microscopic image of the unstained tissue that reveals cell boundaries, such as a differential interference contrast (DIC) image, and an optical quadrature microscopy (OQM) image which is used to prepare an image of optical path length deviation (OPD) across the cell cluster. The boundaries of individual cells in the cell cluster are modeled as ellipses and used, together with the maximum optical path length deviation of a cell, to calculate ellipsoidal model cells that are subtracted from the OPD image. The process is repeated until the OPD image is depleted of phase signal attributable to cells of the cell cluster, and the cell count is obtained from the number of cells subtracted. The method is capable of accurately and non-invasively counting the number of cells in a living embryo at the 2-30 cell stage, and can be employed to assess the developmental stage and health of human embryos for fertility treatments.

This invention relates to a pacifier that may properly interest an infant of eight months old or older to make a preferable stimulation, for leading the infant to the next developmental stage of ingesting activity, or that can be used in a way for adapting to more advanced ingesting activity. A pacifier is provided with a nipple (11) and a shield plate (12) disposed at the base portion (13) of the nipple which has a predetermined width. The nipple is provided with a tip portion (15) having a width and a thickness, the width being larger than the thickness, to be formed into a flat shape, and an upper curved surface (19) formed at the upper surface of the tip portion so as to be convex at a central portion thereof

Gene expression profiling reveals four distinct subgroups of multiple myeloma that have significant correlation with various clinical characteristics. Diagnosis for multiple myeloma (and possibly monoclonal gammopathy of undetermined significance) based on differential expression of 14 genes, as well as prognosis for the four subgroups of multiple myeloma based on the expression of 24 genes are established. A 15-gene model that classifies myeloma into 7 groups is also reported. Gene expression profiling also allows placing multiple myeloma into a developmental schema parallel to that of normal plasma cell differentiation. Development of a gene expression- or developmental stage-based classification system for multiple myeloma would lead to rational design of more accurate and sensitive diagnostics, prognostics and tumor-specific therapies for multiple myeloma.

The present invention relates to novel miRNA molecules, more particularly to novel miRNA molecules isolated from human embryonic stem cells. The miRNA molecules provided by the present invention can be usefully used as a molecular marker for early developmental stages of undifferentiated human embryonic stem cells. Also, the miRNA molecules of the present invention may play an important role in the regulation of mammalian embryonic stem cells. Therefore, the miRNA molecules can be usefully used for analyzing regulatory networks of human embryonic stem cells.

This invention relates to a pacifier that may properly interest an infant of eight months old or older to make a preferable stimulation, for leading the infant to the next developmental stage of ingesting activity, or that can be used in a way for adapting to more advanced ingesting activity. A pacifier is provided with a nipple (11) and a shield plate (12) disposed at the base portion (13) of the nipple which has a predetermined width. The nipple is provided with a tip portion (15) having a width and a thickness, the width being larger than the thickness, to be formed into a flat shape, and an upper curved surface (19) formed at the upper surface of the tip portion so as to be convex at a central portion thereof.

The invention describes a self-renewing, phenotypically homogeneous population of oligodendrocyte precursor cells having a synchronized developmental stage and methods of obtaining a self-renewing phenotypically homogeneous population of oligodendrocyte precursor cells. Other methods include methods of maintaining and storing a homogeneous population of oligodendrocyte precursor cells for a prolonged period of time without change in the characteristics of the cells and methods of dedifferentiating oligodendrocyte precursor cells. The self-renewing, phenotypically homogeneous population of oligodendrocyte precursor cells or homogeneous population of oligodendrocytes may be useful for treating a patient having a CNS disorder or condition.

Increasingly, children are consuming digital content on computers, laptops, tablets, and even smartphones. The sheer volume of content available to children can be daunting for parents to manage and filter for their children. Primitive filtering mechanisms based on a target audience age can be used to eliminate some content based on a child's chronological age. However, children develop at different rates so that even children of the same chronological age can have different developmental stages and so have different preferences for content they consume. Systems and methods described herein provide children with access to developmental stage-appropriate content in a developmental stage-appropriate user interface. The developmental stage-appropriate interface includes representations of the content that are developmental stage-appropriate and surrounding elements that are also developmental stage-appropriate. The user interface can include or not include functional elements based on the developmental stage of the user.

The invention relates to a method for isolating non-embryonic stem cells from a tissue that is located in immediate vicinity of immature, developing teeth or wisdom teeth. The invention further relates to non-embryonic stem cells derived from said tissue. The method according to the invention utilises a living soft tissue residing underneath the dental papilla 12 in immediate vicinity of the apical side of a developing tooth, which is clearly distinguished from other tooth tissue, such as dental papilla 12 or follicle. The pad-like tissue 16 can only be detected in a defined, specific developmental stage in an early phase of root formation. That is, identifying and separating the pad-like tissue 16 is only possible from the appearance of the bony alveolar fundus to the end of the formation of the root of the tooth.

Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The invention further provides transformed plastids having the ability to survive selection in both the light and the dark, at different developmental stages by using genes coding for two different enzymes capable of detoxifying the same selectable marker, driven by regulatory signals that are functional in proplastids as well as in mature chloroplasts. The invention utilizes antibiotic-free selectable markers to provide edible vaccines for conferring immunity to a mammal against Bacillus anthracis, as well as Yersina pestis. The vaccines are operative by parenteral administration as well. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to monocot and dicot plants.

The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to a method determining the developmental stage of human cumulus cells issues from MII oocyte.

The invention discloses a rapid cutting propagation method of crape myrtle single-plant germplasm. On the basis that crape myrtle single plants with the good characters of the natural tree type, flowering habit, flower color and flower diameter size and the like are surveyed and screened out, incising and sprouting promotion is performed on optimized single-plant rootstock portions or purging and sprouting promotion is performed on the surface soil layer lateral roots to form young root sucker coppice shoots at the developmental stage, the coppice shoots are used for ear making and cutting propagation, cutting seedlings are used for building a cutting orchard, cutting orchard building and management are performed by routine, scion wood is completely collected and processed on the base of the trunk of the stock plant to promote sprouting and cultivation of coppice shoots, the standard quality of ear cutting and making is strictly regulated, the cutting seedbed matrix arrangement is simplified, rooting accelerator arrangement and using method are optimized, limitation of the cutting season is broken through, rapid long-term large-scale cutting seedling is achieved, the cutting survival rate and nursery stock quality are improved, and the cutting seedling cost is lowered.

The invention discloses a litchi pollen collecting and storing method, which belongs to the technical field of fruit tree pollination. The method comprises the following steps: 1, collecting litchi anthers which are matured but not cracked at a developmental stage and putting the collected litchi anthers into a glass culture dish; 2, putting the glass culture dish in which the litchi anthers are placed into a drying box and drying the litchi anthers in the glass culture dish by using the drying box; 3, pouring the dried litchi anthers and the scattered pollen into a stainless steel screen, and putting the pollen into a centrifuge tube after enabling the pollen to pass through the stainless steel screen; and 4, sealing the pollen in the centrifuge tube and storing the pollen in an ultra-low temperature refrigerator at -86 DEG C. The method is simple, convenient and easy to implement, and can be used for performing a collecting operation even under different weather conditions. The obtained pollen is high in germination rate and can be stored for the longest time.

Disclosed is a six-component composition of volatile and less-volatile pheromone components that can be used alone or in combination with other compounds to attract and / or arrest bed bugs of both sexes and all developmental stages. This blend can be used to bait traps that are effective in capturing bed bugs in infested premises or can be combined with a pesticide that is lethal to bed bugs.

This invention relates to constructs for the conditional or regulated expression of transgenes in plants using site-specific recombinase systems. The constructs comprise a variety of constitutive, inducible, tissue specific or developmental stage-specific promoters operably linked to either a transgene or the elements of one or more site-specific recombinase system. By matching promoters, responsive to various inducers, plant tissues or plant developmental states with the recombinase systems, stop fragments and transgenes, virtually any trait may be expressed at any plant development stage or in any plant generation.

The present invention relates to a process for the non-destructive determination of gender, developmental stage and / or viability of an avian embryo in an egg, comprising (a) detecting at least a first developmental marker compound selected from sugars and / or amino acids, precursors and metabolites thereof in an egg at a time period of from the beginning of the incubation of the egg until the hatching; (b) measuring the amount of the at least first detected developmental marker compound, and (c) comparing the amount to a base line established for male and female, developmental stage of the embryo, and / or alive and deceased or non-developed embryo, to determine whether the embryo is viable, male and / or female, and / or the developmental stage of the embryo.

A process is provided for inducing direct somatic embryogenesis in Pooideae and rapidly regenerating fertile plants by first culturing isolated immature scutella cells in culture medium comprising auxin, cytokinin and polyamine in amounts effective to cause direct formation of primary embryos without an intervening callus stage, at least until at least one primary embryo reaches the globular developmental stage, the auxin being present in greater proportion than cytokinin. A second step includes either a) culturing the primary embryos under conditions to regenerate plantlets, and culturing the primary embryos in regeneration medium; or b) culturing the primary embryos at the globular developmental stage and no longer than the coleoptilar stage in culture medium comprising auxin, cytokinin, and polyamine in amounts effective to cause induction of secondary embryo formation, at least until secondary embryogenesis is detected, the cytokinin being present in greater proportion than auxin, and culturing the secondary embryos under conditions to regenerate plantlets.

The invention discloses a wheat fertility related gene TaMS7 and an application method thereof, and belongs to the field of biotechnology. The wheat fertility related gene TaMS7 is obtained by analyzing a whole genome expression spectrum of wheat anthers in different developmental stages, and plant fertility is regulated by regulating the expression of the gene. The method is used for producing and maintaining a wheat male sterile line and preparing hybrid seeds. The gene and the method have important theoretical and practical significance for establishing efficient wheat hybrid seed production techniques, and studying wheat male sterility mechanism and heterosis.

The invention belongs to the technical field of analysis of gene expression, and discloses application of a CmEF1 alpha gene and a CmRAN gene used as reference genes in analysis of genetic expression of Cucumis melo L. fruits. The nucleotide sequence of the CmEF1 alpha gene is shown in SEQ ID No:1; the nucleotide sequence of the CmRAN gene is shown in SEQ ID NO:2. The invention further discloses a method for analyzing genetic expression of Cucumis melo L. fruits through adopting real-time fluorescence quantification PCR, and the method includes the step that the geometric mean of the gene combination of the CmEF1 alpha gene and the CmRAN gene is used as the reference genes in analysis of genetic expression of Cucumis melo L. fruits. According to the invention, the CmEF1 alpha gene and the CmRAN gene can be stably expressed in Cucumis melo L. fruits adopting different fruit bearing manners and at different developmental stages, so as to serve as a reliable reference gene combination in analysis of genetic expression during the development process of Cucumis melo L. fruits adopting the real-time fluorescence quantification PCR technology for detection.

The present invention relates to novel miRNA molecules, more particularly to novel miRNA molecules isolated from human embryonic stem cells. The miRNA molecules provided by the present invention can be usefully used as a molecular marker for early developmental stages of undifferentiated human embryonic stem cells. Also, the miRNA molecules of the present invention may play an important role in the regulation of mammalian embryonic stem cells. Therefore, the miRNA molecules can be usefully used for analyzing regulatory networks of human embryonic stem cells.

The invention discloses a method for identifying embryo abortion associated proteins of distant hybridization of chrysanthemum, and belongs to the field of chrysanthemum breeding and proteomics. The method comprises the steps of planting the small chrysanthemum as a female parent, taking wild chrysanthemum nankingense as a male parent for distant hybridization, cutting off pollinated inflorescences at different times after pollination, sampling ovaries with complete and plump morphological development and ovaries with abnormal and hollow morphological development respectively, immediately quick-freezing in liquid nitrogen after sampling, storing in a refrigerator at -80 DEG C, extracting proteins from samples by a TCA (Trichloroacetic Acid) / acetone precipitation method, quickly identifying differential proteins by an iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) and mass-spectrometric technique, performing bioinformatic analysis, and obtaining the embryo abortion associated protein in the distant hybridization breeding of the chrysanthemum. The method can quickly and accurately identify the embryo differential proteins with different morphologies in different developmental stages during embryonic development of the chrysanthemum for the chrysanthemum and affinis wild species of the chrysanthemum, and a research foundation is provided for solving embryo abortion of the distant hybridization of the chrysanthemum.