261 results about "Developmental stage" patented technology

Gene expression profiling reveals four distinct subgroups of multiple myeloma that have significant correlation with various clinical characteristics. Diagnosis for multiple myeloma (and possibly monoclonal gammopathy of undetermined significance) based on differential expression of 14 genes, as well as prognosis for the four subgroups of multiple myeloma based on the expression of 24 genes are established. A 15-gene model that classifies myeloma into 7 groups is also reported. Gene expression profiling also allows placing multiple myeloma into a developmental schema parallel to that of normal plasma cell differentiation. Development of a gene expression- or developmental stage-based classification system for multiple myeloma would lead to rational design of more accurate and sensitive diagnostics, prognostics and tumor-specific therapies for multiple myeloma.

A method and device are provided for counting cells in a sample of living tissue, such as an embryo. The method involves obtaining a microscopic image of the unstained tissue that reveals cell boundaries, such as a differential interference contrast (DIC) image, and an optical quadrature microscopy (OQM) image which is used to prepare an image of optical path length deviation (OPD) across the cell cluster. The boundaries of individual cells in the cell cluster are modeled as ellipses and used, together with the maximum optical path length deviation of a cell, to calculate ellipsoidal model cells that are subtracted from the OPD image. The process is repeated until the OPD image is depleted of phase signal attributable to cells of the cell cluster, and the cell count is obtained from the number of cells subtracted. The method is capable of accurately and non-invasively counting the number of cells in a living embryo at the 2-30 cell stage, and can be employed to assess the developmental stage and health of human embryos for fertility treatments.

An array of multi-stage configured cleansing wipe products may include first stage cleansing wipes and second stage cleansing wipes. The first stage cleansing wipes may have a first structure and a first composition selected in view of a first child developmental stage. The second stage cleansing wipes may have a second structure and a second composition selected in view of a second child developmental stage. The first structure and the first composition are different and potentially progressive from first stage to second stage. First stage product packaging may include at least one first stage-specific indicia and second stage product packaging may include at least one second stage-specific indicia. Stages of the array may be communicated and tailored based upon the foods eaten and the manner of eating (such as being fed or self-feeding) of the babies or children in each stage.

A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

This invention relates to a pacifier that may properly interest an infant of eight months old or older to make a preferable stimulation, for leading the infant to the next developmental stage of ingesting activity, or that can be used in a way for adapting to more advanced ingesting activity. A pacifier is provided with a nipple (11) and a shield plate (12) disposed at the base portion (13) of the nipple which has a predetermined width. The nipple is provided with a tip portion (15) having a width and a thickness, the width being larger than the thickness, to be formed into a flat shape, and an upper curved surface (19) formed at the upper surface of the tip portion so as to be convex at a central portion thereof

The present invention relates to novel miRNA molecules, more particularly to novel miRNA molecules isolated from human embryonic stem cells. The miRNA molecules provided by the present invention can be usefully used as a molecular marker for early developmental stages of undifferentiated human embryonic stem cells. Also, the miRNA molecules of the present invention may play an important role in the regulation of mammalian embryonic stem cells. Therefore, the miRNA molecules can be usefully used for analyzing regulatory networks of human embryonic stem cells.

The invention describes a self-renewing, phenotypically homogeneous population of oligodendrocyte precursor cells having a synchronized developmental stage and methods of obtaining a self-renewing phenotypically homogeneous population of oligodendrocyte precursor cells. Other methods include methods of maintaining and storing a homogeneous population of oligodendrocyte precursor cells for a prolonged period of time without change in the characteristics of the cells and methods of dedifferentiating oligodendrocyte precursor cells. The self-renewing, phenotypically homogeneous population of oligodendrocyte precursor cells or homogeneous population of oligodendrocytes may be useful for treating a patient having a CNS disorder or condition.

Increasingly, children are consuming digital content on computers, laptops, tablets, and even smartphones. The sheer volume of content available to children can be daunting for parents to manage and filter for their children. Primitive filtering mechanisms based on a target audience age can be used to eliminate some content based on a child's chronological age. However, children develop at different rates so that even children of the same chronological age can have different developmental stages and so have different preferences for content they consume. Systems and methods described herein provide children with access to developmental stage-appropriate content in a developmental stage-appropriate user interface. The developmental stage-appropriate interface includes representations of the content that are developmental stage-appropriate and surrounding elements that are also developmental stage-appropriate. The user interface can include or not include functional elements based on the developmental stage of the user.

The invention discloses a litchi pollen collecting and storing method, which belongs to the technical field of fruit tree pollination. The method comprises the following steps: 1, collecting litchi anthers which are matured but not cracked at a developmental stage and putting the collected litchi anthers into a glass culture dish; 2, putting the glass culture dish in which the litchi anthers are placed into a drying box and drying the litchi anthers in the glass culture dish by using the drying box; 3, pouring the dried litchi anthers and the scattered pollen into a stainless steel screen, and putting the pollen into a centrifuge tube after enabling the pollen to pass through the stainless steel screen; and 4, sealing the pollen in the centrifuge tube and storing the pollen in an ultra-low temperature refrigerator at -86 DEG C. The method is simple, convenient and easy to implement, and can be used for performing a collecting operation even under different weather conditions. The obtained pollen is high in germination rate and can be stored for the longest time.

This invention relates to constructs for the conditional or regulated expression of transgenes in plants using site-specific recombinase systems. The constructs comprise a variety of constitutive, inducible, tissue specific or developmental stage-specific promoters operably linked to either a transgene or the elements of one or more site-specific recombinase system. By matching promoters, responsive to various inducers, plant tissues or plant developmental states with the recombinase systems, stop fragments and transgenes, virtually any trait may be expressed at any plant development stage or in any plant generation.

The present invention relates to a process for the non-destructive determination of gender, developmental stage and/or viability of an avian embryo in an egg, comprising (a) detecting at least a first developmental marker compound selected from sugars and/or amino acids, precursors and metabolites thereof in an egg at a time period of from the beginning of the incubation of the egg until the hatching; (b) measuring the amount of the at least first detected developmental marker compound, and (c) comparing the amount to a base line established for male and female, developmental stage of the embryo, and/or alive and deceased or non-developed embryo, to determine whether the embryo is viable, male and/or female, and/or the developmental stage of the embryo.

A process is provided for inducing direct somatic embryogenesis in Pooideae and rapidly regenerating fertile plants by first culturing isolated immature scutella cells in culture medium comprising auxin, cytokinin and polyamine in amounts effective to cause direct formation of primary embryos without an intervening callus stage, at least until at least one primary embryo reaches the globular developmental stage, the auxin being present in greater proportion than cytokinin. A second step includes either a) culturing the primary embryos under conditions to regenerate plantlets, and culturing the primary embryos in regeneration medium; or b) culturing the primary embryos at the globular developmental stage and no longer than the coleoptilar stage in culture medium comprising auxin, cytokinin, and polyamine in amounts effective to cause induction of secondary embryo formation, at least until secondary embryogenesis is detected, the cytokinin being present in greater proportion than auxin, and culturing the secondary embryos under conditions to regenerate plantlets.

The invention discloses a method for identifying embryo abortion associated proteins of distant hybridization of chrysanthemum, and belongs to the field of chrysanthemum breeding and proteomics. The method comprises the steps of planting the small chrysanthemum as a female parent, taking wild chrysanthemum nankingense as a male parent for distant hybridization, cutting off pollinated inflorescences at different times after pollination, sampling ovaries with complete and plump morphological development and ovaries with abnormal and hollow morphological development respectively, immediately quick-freezing in liquid nitrogen after sampling, storing in a refrigerator at -80 DEG C, extracting proteins from samples by a TCA (Trichloroacetic Acid)/acetone precipitation method, quickly identifying differential proteins by an iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) and mass-spectrometric technique, performing bioinformatic analysis, and obtaining the embryo abortion associated protein in the distant hybridization breeding of the chrysanthemum. The method can quickly and accurately identify the embryo differential proteins with different morphologies in different developmental stages during embryonic development of the chrysanthemum for the chrysanthemum and affinis wild species of the chrysanthemum, and a research foundation is provided for solving embryo abortion of the distant hybridization of the chrysanthemum.