85 results about "Developmental stage" patented technology

The invention discloses a specific molecular marker, primers, a method and application for genetic sex identification of siniperca chuatsi, the specific molecular marker has two sequences in male fish and has one sequence in female fish, the primers comprise an upstream primer F and a downstream primer R. The primers are adopted to carry out PCR amplification on genome DNA of the siniperca chuatsi to be detected, and the specific molecular marker is obtained. And identifying the genetic sex of the siniperca chuatsi according to an agarose gel electrophoresis detection result. The invention further discloses application of a reagent, a primer, a kit or a method for detecting the specific molecular marker in genetic sex identification of siniperca chuatsi, artificial breeding of the siniperca chuatsi or all-female breeding of the siniperca chuatsi. The siniperca chuatsi genetic sex identification method has the advantages that sex-related male and female differential fragments are found through combined comparative analysis of male and female genomes, primers are designed for amplification verification, only a small amount of DNA of a to-be-detected sample genome is needed as a template, the genetic sex of the siniperca chuatsi can be accurately, simply, conveniently, quickly and stably identified only through PCR and agarose gel electrophoresis, and the method is not limited by the development stage of the siniperca chuatsi.

The invention discloses application of a ZaSPL7 gene in regulation and control of hairy bodies and parthenocarpy of plants, and belongs to the technical field of gene engineering. The gene ZaSPL7 for regulating and controlling organ development of the green prickleyash is separated from cDNA of the green prickleyash and is overexpressed in a plant, the number and density of epidermal hair of the transgenic plant are obviously increased, meanwhile, the number of fruit seeds is obviously reduced, even part of plants have seedless fruit phenotypes, and the unisexual setting rate is increased. The results show that the green prickleyash SPL7 gene may coordinate epidermal hair and fruit development by integrating development stage signals, and has important application value in regulation and control of epidermal appendages (such as thorns and hair) formation and apomixis, and the mechanism provides a new idea for simultaneous improvement of plant stress resistance (by reducing the thorns) and fruit commodity (by seedless). Important theoretical basis and technical support are provided for breeding of the green prickleyash, and the method has a wide application prospect.

The invention relates to the technical field of molecular biology, in particular to a fluorescent quantitative PCR reference gene screening method in different tissues and stress treatment of perilla frutescens, which specifically comprises the following steps: step 1, respectively selecting perilla frutescens leaves, stalks, roots, fruits at different development stages after flowering and perilla frutescens leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H as samples; extracting total RNA (Ribonucleic Acid) of each sample, and carrying out reverse transcription on the RNA to synthesize cDNA (Complementary Deoxyribose Nucleic Acid) as And step 2, screening out candidate reference genes of the purple perilla by using the sequencing data of the early-stage purple perilla transcriptome, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH respectively, and screening out the candidate reference genes of the purple perilla, namely PfActin, Pf18S, PfEF-1alpha, PfCYP, PfTUA, PfTUB and PfGAPDH. According to the method, the most stable reference genes in perilla leaves, stems, roots, fruits in three development stages and perilla leaves treated by 20% PEG6000 for 0H, 3H, 6H, 9H, 12H and 24H are screened from seven candidate reference genes, and a foundation is laid for deep excavation of functional genes in the later period.

The invention discloses a mouse embryo quality and development stage combined prediction system and method based on deep learning, and the system comprises a data preprocessing module which is used for obtaining a time sequence data set, and a feature extraction module which is used for processing the time sequence data set through a pre-trained OfficientNet model, and obtaining the feature vector of an embryo image corresponding to each time point; the time sequence feature fusion module is used for processing the image feature vectors of all embryos arranged according to the time sequence by using a bidirectional long-short-term memory network to obtain hidden state vectors of all embryo image fusion time sequence information; and the multi-task prediction module is used for inputting the hidden state vector into the embryo quality prediction and development stage classification prediction model after joint optimization to obtain a splicing result of embryo quality prediction and development stage classification prediction. According to the method, two prediction tasks of embryo quality and development stage classification can be processed at the same time by utilizing the combination of the OfficientNet model and the bidirectional long-short-term memory network, so that the model processing efficiency is improved.

The present invention relates to a method for plant genome modification of at least one plant cell being in the developmental stage of a plant immature inflorescence meristem (IIM) cell, wherein the modification of the specific cell type is achieved by providing a genome modification or editing system, optionally together with at least one regeneration booster, preferably wherein the effector molecules are introduced by particle bombardment. To this end, new artificial and precisely controllable booster genes and proteins are provided. Further, the modified plant cells are regenerated in a direct or an indirect way. Finally, methods, tools, constructs and strategies are provided to effectively modify at least one genomic target site in a plant cell, to obtain the modified plant cell and to regenerate a plant tissue, organ, plant or seed from the modified plant cell.

The invention discloses a bombyx mori protein function prediction multi-modal fusion method based on a deep residual network, and the method comprises the steps: collecting the sequences and high-resolution three-dimensional structure data of bombyx mori proteins of different varieties and at different development stages from databases of bombyx mori genomes, protein crystal structures and the like by employing a data crawling and interface calling technology; and constructing a multi-modal data source. The sequence data are cleaned and coded in a one-hot mode, and the structural data are optimized and converted into a graph structure. Sequence and structure features are respectively extracted through a convolutional neural network and a GraphSAGE graph neural network, and the features are dynamically fused through an adaptive weight fusion strategy. And building a deep residual network training model, and realizing accurate prediction of the protein function category and confidence of the bombyx mori with unknown functions. According to the method, the precision and the high robustness of silkworm protein function prediction are improved through full-process technical optimization, and an efficient tool is provided for silkworm molecular breeding and functional genomics research.

The invention discloses a novel codon optimization method based on precise transcriptome sequencing and analysis thereof, and belongs to the technical field of bioinformatics. In order to improve the expression quantity of a target gene, corresponding accurate transcriptome data is obtained according to the development period of transgenic expression and parts such as tissues and organs, and efficient expression genes are screened to serve as a host genome expression library; the expression abundance of the gene is comprehensively considered when the use frequency of the codon of the host genome is determined, and the gene optimized by the novel codon optimization method increases the stability of the insecticidal protein gene RNA and improves the expression level of the protein. The invention provides better reference for gene modification and transformation, and provides a novel codon optimization method for high-efficiency expression of transgenes in plants.

The invention discloses a reference gene combination stably expressed by hermetia illucens under different conditions and application thereof, reference genes stably expressed by hermetia illucens in different development stages, different tissues, different genders and different genotypes are obtained through screening, and the reference gene combination is suitable for correction of hermetia illucens RT-qPCR detection results under different experimental conditions. Meanwhile, the invention further provides amplification primers for detecting the hermetia illucens reference genes, and the amplification primers are high in specificity and amplification efficiency. According to the invention, an early-stage foundation is laid for the quantification of related functional genes of hermetia illucens under different experimental conditions and the related research of molecular biology.

The present invention discloses a molecular marker, primer and application thereof for identifying the color of Actinidia arguta fruit. The molecular marker of the present invention is located at 495bp upstream of the start codon of the AaLDOX gene promoter region of Actinidia arguta, which is a 29bp Indel variation. Utilizing the molecular marker and method of the present invention, the fruit color can be quickly and accurately identified in the childhood stage of Actinidia arguta by conventional molecular test detection, and the red and green Actinidia arguta germplasm resources can be efficiently distinguished. The method can be used for parent selection and seedling identification of offspring, greatly improving the efficiency and accuracy of selective breeding. The method of the present invention can be detected in each tissue, organ and each developmental stage of the plant, and has the advantages of being convenient and fast, having good specificity, high accuracy, and being unaffected by climate and environment. It can be applied to the precise, directional and efficient breeding of red Actinidia arguta, and has a large application value.

Provided is a method for obtaining the germ cell lineage of an oviparous vertebrate more efficiently than conventional techniques. A host oviparous vertebrate is prepared at a developmental stage after the development of black pigmentary cells in the retina and before the formation of multiple layers of germ cells in the genitals, and isolated undifferentiated germ cells from a donor oviparous vertebrate are transplanted into the host oviparous vertebrate.

This invention discloses an indoor breeding method for ladybugs, belonging to the field of biological control. This invention uses beet armyworms to partially replace whiteflies, the natural food source for ladybugs at different developmental stages, thereby reducing the amount of whiteflies required. Since the artificial feeding technology for beet armyworms is currently very mature, beet armyworm eggs can be obtained stably and efficiently. Therefore, this invention, by using beet armyworm eggs to partially replace whiteflies, the natural food source for ladybugs, simplifies the indoor breeding process of ladybugs, reduces breeding costs, and has good application prospects.

The present invention discloses a SNP molecular marker associated with upland cotton linolenic acid and its application. The SNP molecular marker is at least one of the nucleotide sequences shown in SEQ ID NO.1-SEQ ID NO.28, and the SNP molecular site mutates at the 51st bp of the sequence. The SNP molecular marker associated with upland cotton linolenic acid provided by the present invention can be used for early prediction and screening of cotton linolenic acid traits. It is directly expressed in the form of DNA and can be detected in various tissues and developmental stages of cotton. It is not restricted by season, environment, or expression; it is neutral and does not affect the expression of target traits; SNP is suitable for rapid and large-scale screening. In genomic screening, SNPs often only require + / - analysis without analyzing the length of the fragment, which is conducive to the development of automated technology to screen or detect SNPs.

This invention discloses a primer pair for identifying the barbaric harvester ant and its application, relating to the field of molecular biology. The invention discloses primer pairs with nucleotide sequences as shown in SEQ ID NO:5 and SEQ ID NO:6. It overcomes the limitations of traditional morphological detection and can effectively distinguish the barbaric harvester ant from nine closely related species (*C. spp.*, *C. brevicornu*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, *C. spp.*, and *C. spp.*). It is suitable for detecting different developmental stages of the barbaric harvester ant, achieving rapid, accurate, and stable detection with a sensitivity as low as 0.01 ng / μL.

The invention belongs to the technical field of crop variety breeding in modern agriculture, and discloses a method for creating homozygous tetraploid rice by using haploid anther, which makes full use of the characteristic that the number of chromosomes in cells subjected to meiosis is halved, and microspores formed by meiosis are subjected to anther culture at a proper development stage, so that the homozygous tetraploid rice is created. The method comprises the following steps of: a, selecting a diploid rice line with excellent characters as a parent material, and carrying out continuous secondary induction by colchicine to form a callus, and differentiating to emerge seedlings to form a stable homozygous tetraploid rice line; b, selecting anther with microspores in the late uninucleate stage during observation; c, inducing anther calluses; e, differentiating the anther calluses to emerge seedlings; f, seedling callus differentiation and rooting; and g, determining the authenticity of a homozygous tetraploid plant formed by anther culture through morphological and cytological technologies (flow cytometry). The method is simple, low in cost and easy to operate.

The invention discloses a method for identifying a pluripotent state of a cattle epidermal layer, and achieves the technical effect of identifying the pluripotent state of a cattle embryo, especially the pluripotent state of the cattle epidermal layer. The invention specifically discloses gene markers for different pluripotent stages and different developmental stages, and the pluripotent and developmental stages of to-be-detected bovine embryonic cells or cell clusters can be rapidly identified by using the gene markers for different pluripotent stages and different developmental stages of bovine embryos.

The present invention discloses SNP molecular markers associated with cotton fiber strength quality traits and their applications, belonging to the fields of molecular biology and bioinformatics. The SNP molecular marker is at least one of the nucleotide sequences shown in SEQ ID NO.1-SEQ ID NO.126. The present invention provides the SNP molecular markers associated with upland cotton fiber strength, which can be detected in various tissues and developmental stages of cotton, are not restricted by the environment and season, are not affected by expression, do not need to analyze the length of the fragment, only need + / - analysis, are suitable for rapid, large-scale, and automated screening, and can be used alone or in combination to assist in identifying high- and low-strength fiber germplasm materials, which can greatly save the work of breeding and improvement.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of the PoPILS1a gene in regulating plant seed development. The coding sequence of the PoPILS1a gene is shown in SEQ ID NO:1. This invention is the first to discover that PoPILS1a is specifically expressed in peony seed tissues, and the expression level varies significantly at different developmental stages. The PoPILS1a protein is located in the endoplasmic reticulum. In Arabidopsis thaliana positive lines with overexpression of PILS1a, the attachment point between pods is significantly shortened, and the number of pods distributed within a limited inflorescence length is greater. Simultaneously, the length and weight of pods at corresponding positions show significant differences compared to the control, clarifying that PILS1a can promote fruit development and has good practical application value.

We provide the system. [Solution] A means for capturing video of the baby and sending it to a server, A means for detecting the baby's behavior from the video data using an image analysis algorithm, A means for evaluating the baby's developmental stage based on the detected behavior using a machine learning model, A means for generating childcare advice based on the aforementioned evaluation results, A means for transmitting and displaying the aforementioned childcare advice on a terminal, A system that includes this.

The invention discloses a lycoris radiata LlMYB6 gene as well as an expression protein and application thereof, and belongs to the technical field of plant molecular biology. The invention provides a lycoris radiata LlMYB6 gene, the nucleotide sequence of the lycoris radiata LlMYB6 gene is shown as SEQ ID NO.1 in a sequence table, and the amino acid sequence of the lycoris radiata LlMYB6 gene is shown as SEQ ID NO.2 in the sequence table. Expression modes of LlMYB6 in three different development stages of pink lycoris longtube flower slices are quantitatively determined in real time through fluorescence, and functions of the gene are identified through subcellular localization, stable tobacco transformation, dual-luciferase detection and GUS activity detection. It is determined that the transcription factor LlMYB6 can negatively regulate synthesis and accumulation of lycoris radiata anthocyanin by inhibiting expression of the enzyme gene LlPAL, and an effective molecular tool is provided for improving plant flower color through genetic engineering.

The present invention discloses a system and a method for detecting different developmental stages of fungi life cycle in postharvest crops. More particularly, the system comprises a measuring transducer, such as a CMOS photodetector and immobilized fungal nucleic acid strands. RNA from the tested postharvest produce sample is loaded onto the system of the present invention, and specific fungal mRNA transcripts anneal to the immobilized strands. A reporter strand conjugated to a signal-generating component further binds the mRNA transcripts, and when a detectable reaction is produced post binding, the transducer captures said reaction and converts it to measurable values, indicating the quantity, presence and the developmental stage of the fungus.

This invention discloses a preprocessing method for the spatial transcriptome of maize anthers based on morphological restoration and precise permeation. The method includes: attaching and fixing maize anther tissue sections onto a spatial transcriptome capture chip; staining with a preferred concentration (1 / 200) of fluorescent whitening agent (FB28), followed by moist incubation to allow the anther exowall cells to absorb water and reshape; imaging and localization while maintaining chip surface hydration throughout the process; precise permeation treatment for 7 minutes using a permeation reagent; and finally, in situ cDNA synthesis. This invention effectively overcomes the localization challenge caused by maize anther tissue shrinkage through the synergistic effect of staining and restoration processes. Combined with optimized permeation parameters, it solves the permeation problem at different developmental stages of anthers, significantly improving the spatial resolution of sequencing data and making it suitable for spatial transcriptome studies of the entire developmental cycle of maize anthers.

This invention relates to the field of molecular biology, specifically to an internal reference gene of whorled holly and its application, and particularly to... Actin2 and / or 28S As an internal reference gene for quantitative analysis of Ilex chinensis genes, the aforementioned Actin2 The nucleotide sequence is shown in SEQ ID NO.1. 28S The nucleotide sequence is shown in SEQ ID NO.2. This invention provides an internal reference gene for analyzing the expression of target genes in samples from different tissues of *Ilex verticillata* at different developmental stages and under different hormone treatments. Actin2 and / or 28S The study also included the detection of primer pairs. This research provides technical support and reliable evidence for the study of gene expression characteristics in whorled holly and subsequent research related to flower buds.

The invention discloses a mulberry heat shock transcription factor MaHSF1 as well as a coding gene and application thereof, and aims to solve the technical problem that high-temperature-resistant gene resources and germplasm resources of mulberry are deficient at present. On the basis of the heat shock transcription factor MaHSF1 which is screened from a mulberry HSF gene family and is obviously subjected to high-temperature stress up-regulation and heterologous expression of which can improve the high-temperature resistance of the yeast, the over-expression of the heat shock transcription factor MaHSF1 can inhibit the increase of the content of malondialdehyde MDA at high temperature; the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and the content of osmotic regulation substance free proline are improved, so that the high-temperature resistance of arabidopsis thaliana is improved; the expression of the promoter has developmental stage specificity and heat stress induction characteristics, which indicates the accuracy of the regulation mechanism of the promoter. According to the application, the function and the action mechanism of the MaHSF1 and the coding gene thereof for resisting the high-temperature stress damage are verified for the first time, and a solid theoretical basis is provided for the mulberry production to cope with the high-temperature stress damage and the breeding of new high-temperature-resistant varieties.

Disclosed herein include methods and compositions for culture medias for in vitro culture of synthetic embryos from mammalian pluripotent stem cells and extra-embryonic stem cells. The methods and compositions described herein can generate synthetic embryos at different developmental stage reaching early organogenesis and beyond. Disclosed herein also include an embryo culturing system and methods of using same.

The present invention relates to the application of ApABCG4 in regulating the drought tolerance of aphids and biological control, including determining the expression profiles of ApABCG4 in different developmental stages and tissues of pea aphids, as well as the effect of drought stress on its expression. RNAi was used to inhibit the expression of ApABCG4, and the effects of its silencing on the abdominal cuticle of aphids and the drought tolerance of aphids were determined. The results showed that after the silencing of ApABCG4, the drought tolerance of pea aphids could be significantly reduced. It can be used as a biological control target to effectively regulate the drought tolerance of pea aphids, and substances such as dsRNA that can inhibit the expression level of the ApABCG4 gene were designed and used as pea aphid inhibitors, providing new ideas and methods for the green control of pea aphids.

A Solanaceous plant exhibiting a parthenocarpy and down-regulation in activity or expression of MBP3 and at least one of AGL6 and MBP22, as compared to a control plant, wherein the control plant is of the same genetic background and developmental stage. Also provided are methods of producing the plant and uses thereof.

The invention discloses a method for constructing a crop leaf age simulation model, and the method comprises the following steps: (1) collecting the phenological data of a to-be-simulated crop at a research site, and obtaining the day order of the beginning of the development period of the to-be-simulated crop, all key development stages, and the meteorological data of the research site; (2) according to the data obtained in the step (1), continuously calculating three types of development period process simulation values of the to-be-simulated crop under a day-by-day or hour-by-hour scale through a temperature difference method, a temperature accumulation method and a physiological development time method, and obtaining leaf age simulation values under the three types of development period processes by utilizing a quantitative relation between leaf age and the three types of development period process simulation values; and (3) integrating the leaf age simulation values obtained in the step (2) under the three developmental period processes to obtain a leaf age integrated simulation value of the to-be-simulated crop. The crop leaf age simulation model constructed by the method is high in precision, and technical support is provided for intelligent production management of horticultural crops.

The invention provides a reference gene screening method for fluorogenic quantitative PCR (Polymerase Chain Reaction) analysis of peach-necked longhorn beetles and application of the reference gene screening method. The specific scheme is as follows: RPL13 and RPL10 are selected as reference gene combinations in different development stages, and alpha-tubulin and SOD are selected as reference gene combinations in different tissues. The nucleotide sequences are respectively ID 1, ID 2, ID 3 and ID 4. In addition, the invention also provides different primer sequences used when the stably expressed reference gene is used for qRT-PCR (quantitative reverse transcription-polymerase chain reaction) analysis. The method comprises the following steps: selecting 10 candidate genes, applying a qRT-PCR (quantitative reverse transcription-polymerase chain reaction) technology, combining GeNorm, BestKeeper, a delta Ct method, NormFinder and RefFinder software for analysis, and screening out a reference gene combination which is stably expressed under different experimental conditions. The screening system provides important technical support for the gene function research of the peach-necked longhorn beetles, and provides a scientific basis for the research of the peach-necked longhorn beetles by utilizing molecular biology.

The system (100) and method for simulation and understanding embryology, as compared to prior-arts, is capable to help understand embryology by simulation. System (100) and method includes a processing system (10) that has an interactive module (20) which receives selection of a user from a general embryology module (20a), a systemic embryology module (20b), a genetics module (20c), and a stem cells module (20d). The system (100) and method also includes a text module (30) to display text, an audio-video module (40) to play a three-dimensional simulation module (50) to allow simulation, a fetal surgery and intervention simulation module (60) and a comparative module (70) to allow comparison of fetal anatomical structures and physiology at different developmental stages of age to adults.

A lentivlral vector for real-time monitoring of cell state which includes a regulatory element that regulates expression of a selection marker, wherein the regulatory element comprises cell-state-specific histone H3K27-acetylation marks and / or chromatin accessibility. Methods of identifying a cell- state-specific regulatory element in a cell; optically screening a compound for the ability to modify cell identity; in vivo labeling normal and / or diseased cells at a specific developmental stage or a specific stage of disease; and real- time monitoring of transcription of a cell exposed to a stress using the lentiviral vector.