Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for large scale preparing Gliocladium chlamydospore

A technology of chlamydospores and Gliocladium, which is applied in the field of microorganisms and can solve problems such as short survival period

Active Publication Date: 2009-06-10
北京启高生物科技有限公司
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hyphae of Gliocladium are prone to dehydration and cause death under natural conditions, and although the conidia produce a large amount of spores, their survival period is also short, and usually 70% of the spores lose their vitality after a one-year storage period; Apparently, the two types of bacteria, conidia and mycelia, are difficult to meet the requirements of Gliocladium as a biopesticide preparation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: the formula for preparing a large amount of Gliocladium chlamydospores

[0013] components Content (volume per liter) glucose 20 grams bean cake powder 1 g urea 3 grams ferrous sulfate 0.05 grams Dipotassium phosphate 1 g distilled water 1 Litre

[0014] Add glucose, bean cake powder, urea, ferrous sulfate, and dipotassium hydrogen phosphate to distilled water in sequence according to the above proportions, and stir while adding until all components are completely dissolved and mixed evenly.

Embodiment 2

[0015] Embodiment 2: the formula for preparing a large amount of Gliocus chlamydospores

[0016] components Content (volume per liter) glucose 25 grams bean cake powder 2 grams urea 2 grams ferrous sulfate 0.01g Dipotassium phosphate 1 g distilled water 1 Litre

[0017] Add glucose, bean cake powder, urea, ferrous sulfate, and dipotassium hydrogen phosphate to distilled water sequentially according to the above proportions, and stir while adding until all components are completely dissolved and mixed evenly.

Embodiment 3

[0018] Embodiment 3: the method for cultivating Gliocladium chlamydospores by liquid fermentation

[0019] components Content (volume per liter) glucose 25 grams bean cake powder 2 grams urea 2 grams ferrous sulfate 0.01g Dipotassium phosphate 1 g distilled water 1 Litre

[0020] Add glucose, bean cake powder, urea, ferrous sulfate, and dipotassium hydrogen phosphate to distilled water sequentially according to the above proportions, and stir while adding until all components are completely dissolved and mixed evenly. 121 DEG C of high-pressure damp heat sterilization for 30 minutes, when the sterilized culture solution was cooled to room temperature, insert the conidia liquid of Gliocladium in the amount of 5% (volume ratio) (concentration is 10 5 / ml), after 48 hours of shaking and dark cultivation at 25°C, a fermentation broth containing a large amount of chlamydospores was obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing a large quantity of chlamydospore of Gliocladium virens, which comprises a culture medium formula and culture technology. A liquid medium comprises the following compositions: 20 to 25 grams per liter of glucose, 1 to 3 grams per liter of bean cake powder, 1 to 3 grams per liter of urea, 0.005 to 0.05 gram per liter of ferrous sulfate, 0.75 to 1.5 grams per liter of dipotassium hydrogen phosphate and distilled water. The liquid medium is used for fermentation culture of Gliocladium virens which have superparasitic function on various plant pathogenic fungi (such as sclerotinia, gray mold, banded sclerotial blight and pine root fungi). The culture time is calculated beginning from inoculation, and the chlamydospore of the Gliocladium virens begins to be greatly generated after 36 to 48 hours and is respectively and independently separated. The invention aims to provide the culture medium formula with lower price and rich sources of raw materials, wherein a large quantity of the chlamydospore of the Gliocladium virens can be prepared by utilization of the culture medium formula; and the repeatability is good and the cost is greatly reduced under the condition of liquid fermentation culture, so as to guarantee the biological activity and the prevention and treatment effect of the spores in production and application.

Description

Technical field: [0001] The invention relates to the technical field of microbes, in particular to a method for preparing a large amount of Gliocladium chlamydospores, including medium formula and culture technology. Background technique: [0002] In the biopesticides used to control plant diseases, microbial fungal preparations play an important role. Among them, Gliocladium spp. is considered to be one of the most promising plant disease biocontrol factors among the antagonistic microorganisms discovered so far, and can be used to control various plant diseases. [0003] Gliocladium is a soil habituating fungus that widely exists in various soil ecological environments. Common Gliocladium species include G. roseum, G. catenulatum and G. virens, etc., which have shown good control effects on plant diseases. To sum up, Gliocladium has great biocontrol potential due to its excellent characteristics as follows: ① It has a wide range of environmental adaptability, can use var...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14
Inventor 张拥华张保元李世东
Owner 北京启高生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products