Method for large scale preparing Gliocladium chlamydospore
A technology of chlamydospores and Gliocladium, which is applied in the field of microorganisms and can solve problems such as short survival period
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Embodiment 1
[0012] Embodiment 1: the formula for preparing a large amount of Gliocladium chlamydospores
[0013] components Content (volume per liter) glucose 20 grams bean cake powder 1 g urea 3 grams ferrous sulfate 0.05 grams Dipotassium phosphate 1 g distilled water 1 Litre
[0014] Add glucose, bean cake powder, urea, ferrous sulfate, and dipotassium hydrogen phosphate to distilled water in sequence according to the above proportions, and stir while adding until all components are completely dissolved and mixed evenly.
Embodiment 2
[0015] Embodiment 2: the formula for preparing a large amount of Gliocus chlamydospores
[0016] components Content (volume per liter) glucose 25 grams bean cake powder 2 grams urea 2 grams ferrous sulfate 0.01g Dipotassium phosphate 1 g distilled water 1 Litre
[0017] Add glucose, bean cake powder, urea, ferrous sulfate, and dipotassium hydrogen phosphate to distilled water sequentially according to the above proportions, and stir while adding until all components are completely dissolved and mixed evenly.
Embodiment 3
[0018] Embodiment 3: the method for cultivating Gliocladium chlamydospores by liquid fermentation
[0019] components Content (volume per liter) glucose 25 grams bean cake powder 2 grams urea 2 grams ferrous sulfate 0.01g Dipotassium phosphate 1 g distilled water 1 Litre
[0020] Add glucose, bean cake powder, urea, ferrous sulfate, and dipotassium hydrogen phosphate to distilled water sequentially according to the above proportions, and stir while adding until all components are completely dissolved and mixed evenly. 121 DEG C of high-pressure damp heat sterilization for 30 minutes, when the sterilized culture solution was cooled to room temperature, insert the conidia liquid of Gliocladium in the amount of 5% (volume ratio) (concentration is 10 5 / ml), after 48 hours of shaking and dark cultivation at 25°C, a fermentation broth containing a large amount of chlamydospores was obtained.
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