92 results about "Dipotassium phosphate" patented technology

The invention provides a culture medium formula capable of effectively improving vibrio inhibition capability of bacillus. The culture medium formula comprises 10-20 g of corn starch, 2-10 g of yeast powder, 2-10 g of malt extract, 5-20 g of glycerin, 0.5-1 g of L-aspartic acid, 1 g of dipotassium phosphate, 0.3 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 1 g of calcium carbonate and 1000 mL of distilled water. When the culture medium is used for culturing bacillus capable of naturally inhibiting vibrio, the obtained culture has a better vibrio inhibiting effect.

The invention relates to the technical field of production of dipotassium phosphate, discloses a process method for continuously producing food-grade anhydrous dipotassium phosphate, aims to obtain a food-grade anhydrous dipotassium phosphate product through a drying reaction in a rotary polymerization furnace by a one-step method, and effectively solves the problems of multiple control procedures, complicated process control and high production cost. The coke content is greatly reduced under the condition of ensuring the dipotassium phosphate content.

The invention relates to the technical field of microorganisms, and discloses an induction method of VBNC state shigella, a resuscitation medium and application, and the resuscitation medium comprises Rpf recombinant protein, MgSO4, sodium pyruvate, vitamin B12, tryptone, glucose, dipotassium phosphate, monopotassium phosphate, sodium chloride and Tween 80. The resuscitation culture medium obtained by the invention can be used for successfully culturing Shigella in a VBNC state.

The invention discloses a dental whitening gel and a preparation method thereof, and belongs to the technical field of oral medicine. Comprising the following components in percentage by mass: 10%-20% of urea peroxide, 0.5%-1% of sodium alginate, 1%-2% of tea tree oil, 2%-3% of dipotassium phosphate, 2%-4% of monopotassium phosphate, 3%-5% of carbomer, 3%-5% of glycerol, 59%-78% of deionized water and 0.5%-1% of pentasodium diethylenetriamine pentaacetate. The oral cavity cleaning agent has high safety, and does not have serious toxicity and irritation to the oral cavity.

The present invention belongs to the technical field of rapid detection of drug sensitivity for bacteria. Disclosed are an enrichment culture medium, and a preparation method therefor and the use thereof. The enrichment culture medium comprises the following raw materials in parts by mass: 15.0-20.0 parts of tryptone, 3.5-5.5 parts of peptone, 1.5-3.5 parts of glucose, 5.0-6.5 parts of yeast extract, 2.0-2.5 parts of dipotassium phosphate, 2.0-3.5 parts of disodium hydrogen phosphate, 1.0-1.5 parts of monopotassium phosphate, 2.5-7.5 parts of sodium chloride, 12.0-18.0 parts of proteose peptone, 15.0-22.0 parts of beef heart infusion powder, 8.0-12.0 parts of soya peptone, 1.0-5.0 parts of 0.1% nicotinamide adenine dinucleotide (NAD), 30-100 parts of newborn calf serum inactivated at 56°C for 30 min, 2.0-4.0 parts of mannitol, 3.0-5.0 parts of sodium pyruvate, 0.23-0.45 parts of cycloheximide, 0.8-2.0 parts of 0.05% bromothymol blue, 2.5-4.5 parts of 0.1% methylene blue, and 1000 parts of distilled water, with the pH value of 6.8-7.5. The enrichment culture medium is used to screen effective therapeutic drugs for bacterial diseases in poultry, and is convenient to use and has a single enrichment effect superior to that of respective selective enrichment broths of target bacteria. Therefore, the culture medium can realize rapid proliferation of common pathogenic bacteria in poultry.

The present invention discloses a cattle fattening and growth promoting compound preparation and a preparation method thereof, and particularly relates to the technical field of livestock breeding, the cattle fattening and growth promoting compound preparation comprises 30-40 parts of rhizoma atractylodis, 30-40 parts of radix astragali, 10-20 parts of cortex magnoliae officinalis, 10-20 parts of jujube, 5-15 parts of coated guanidinoacetic acid, 3-5 parts of a compound probiotic liquid, soybean meal accounting for 2% of the total mass of the compound preparation, corn flour accounting for 1% of the total mass of the compound preparation, and the balance of water. The molasses accounts for 1% of the total mass of the compound preparation, and the dipotassium phosphate accounts for 0.5% of the total mass of the compound preparation. The preparation method comprises the following steps: S1, pretreating the traditional Chinese medicines; S2, mixing the raw materials; S3, adding bacterial liquid and enveloped guanidinoacetic acid; and S4, fermenting. According to the compound preparation, the growth speed of cattle and the feed conversion rate can be remarkably increased, the immunity of the cattle is enhanced, the health of the cattle is guaranteed, meanwhile, green and safe breeding is achieved, in addition, the preparation technology is simple and easy to operate, and the quality and stability of the preparation can be guaranteed.

The present invention provides low glycemic index (GI) total nutrition formula emulsion and a preparation method therefor. The low GI total nutrition formula emulsion comprises the following raw material components in parts by weight: 10-14 parts of carbohydrates, 4-5 parts of calcium caseinate, 1.3-2 parts of dietary fiber, 0.1-0.15 part of sodium chloride, 0.1-0.12 part of sodium citrate, 0.1-0.15 part of potassium citrate, 0.15-0.2 part of magnesium chloride, 0.1-0.15 part of dipotassium hydrogen phosphate, 0.05-0.1 part of compound vitamins, 0.05-0.1 part of compound minerals, 2.5-3.6 parts of compound oil, 0.3-0.5 part of phospholipid, 0.2-0.5 part of medium chain triglyceride (MCT), 0.04-0.08 part of colloids. The preparation method comprises: preparing aqueous phases and oil phases from the raw materials step by step; mixing the aqueous phases and oil phases; and shearing, homogenizing and sterilizing the mixture to obtain the low GI total nutrition emulsion. The product features balanced and complete nutrition.

The invention discloses a haemophilus parasuis high-density fermentation culture medium as well as a culture method and application thereof, and relates to the technical field of microbial fermentation. The haemophilus parasuis high-density fermentation culture medium is prepared from the following components according to the content in 1L: 1.8 to 2g / L of glucose, 20 to 25g / L of organic nitrogen source, 2.0 to 3.0 g / L of dipotassium phosphate, 4.5 to 5.5 g / L of sodium chloride, 0.5 to 1.8 mg / L of NAD (Nicotinamide Adenine Dinucleotide), 8.2 to 8.6 mL / L of fetal calf serum and the balance of water, and the pH value is 7.0 to 7.5; wherein the organic nitrogen source is composed of Oxoid yeast and green source soybean peptone in a mass ratio of 1: 2. By optimizing the proportion of the glucose, the organic nitrogen source and the fetal calf serum, the viable count of the haemophilus parasuis is remarkably increased, when the culture medium is used for fermentation culture, OD600 can reach 1.8 through 7-hour fermentation, the viable count reaches 1010 CFU / mL, the viable count is increased by more than one time compared with that of a traditional culture medium, and the culture medium is suitable for large-scale production. And a high-density thallus raw material is provided for vaccine production.

The invention relates to the technical field of pathogenic microorganism detection, and discloses a helicobacter pylori detection kit based on a urease method and a preparation method, the helicobacter pylori detection kit comprises a detection sleeve card, detection test paper and a tartar sampling label, the test paper is soaked in a reaction solution and a buffer solution in the preparation process of the detection test paper, the pH of the buffer solution is 6.5-7.0, and the tartar sampling label is used for sampling tartar. The buffer solution comprises monopotassium phosphate and dipotassium phosphate, the final concentration of the monopotassium phosphate is 14-30 g / L, and the final concentration of the dipotassium phosphate is 1.8-3.6 g / L. The method can solve the problems of high false positive rate of detection results, poor accuracy, slow reagent response speed and the like caused by complex oral environment (such as diet residue and non-specific bacterial interference) and lack of an effective control system when a tartar sample is used for detecting helicobacter pylori in the prior art.

The invention provides a method for rapidly determining the vitality of blueberry pollen, and relates to the technical field of blueberry pollen vitality detection. The method for rapidly determining the vitality of the blueberry pollen comprises the following specific steps: step 1, pollen collection: when blueberry flowers are in a big flower bud stage, picking the flowers into a self-sealing bag, putting the self-sealing bag into a preservation box with ice cubes, taking the preservation box back to a laboratory, and performing detection on the day; and step 2, preparing a PBS solution, namely fixing the volume of 3.16 g of monopotassium phosphate to 100 ml, fixing the volume of 2.28 g of dipotassium phosphate to 100 ml, preparing a mixed solution, and adding an acidic titration solution or an alkaline titration solution to prepare the PBS solution with the pH value of 7.0. TTC (2, 3, 5-triphenyltetrazole chloride) is dissolved in a PBS solution, a 1% TTC solution is prepared for dyeing, the blueberry pollen suspension prepared by the method can fully absorb a reaction solution to avoid pollen viability errors caused by individual differences, meanwhile, the blueberry pollen dyeing time can be 20 min, and the blueberry pollen viability detection time is greatly shortened.

The invention relates to the technical field of tooth restoration, in particular to a composition for repairing tooth leukoplakia and a preparation method of the composition. The composition for repairing the tooth leukoplakia comprises the following components: artificial saliva, and the artificial saliva comprises 0.2 g / L to 1 g / L of dipotassium phosphate and 0.2 g / L to 1 g / L of calcium chloride. Furthermore, the composition also comprises an active glass composition. By adopting the technical scheme, the artificial saliva containing phosphate radicals, potassium ions and calcium ions with specific concentrations can be matched with permeable resin, an active glass composition and the like to achieve a good tooth leukoplakia repairing effect, so that the technical problem that the application of permeable resin is limited in the prior art is solved.

The invention discloses a bone regeneration multiple bionic scaffold material and a preparation method thereof, and relates to the technical field of bionic bones. According to the bone regeneration multi-bionic scaffold material and the preparation method thereof, a polylactic acid block copolymer is used as a raw material, a 3D printing technology is adopted to prepare a substrate scaffold meeting the bone defect morphology, and then the substrate scaffold is alternately soaked in a collagen mixed solution and a modified layered dihydroxide solution for multiple times to obtain the bone regeneration multi-bionic scaffold material. A bionic matrix layer composed of collagen / layered dihydroxide is formed on the surface of the base stent, and finally, the base stent with the bionic matrix layer attached to the surface is alternately soaked in dipotassium phosphate and calcium chloride solutions for multiple times in sequence; and performing in-situ mineralization on the substrate support with the bionic matrix layer attached on the surface to form calcium phosphate nanoparticles, and finally cleaning and drying to obtain the calcium phosphate nano-particles. The bone regeneration multi-bionic scaffold material prepared by the preparation method disclosed by the invention is relatively good in biocompatibility and relatively good in mechanical property.

The invention relates to a humic acid water-soluble fertilizer as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The humic acid water-soluble fertilizer comprises a base fertilizer raw material and a thin film layer, wherein the base fertilizer raw material comprises the following components: humate, urea, dipotassium phosphate, potassium sulfate, calcium nitrate, magnesium sulfate, sodium dodecyl benzene sulfonate, carboxymethyl cellulose sodium and propylene glycol; the raw material of the thin film layer is a mixture of alginate oligosaccharide, cinnamon oil and amino acid; the amino acid mixture consists of phenylalanine and glutamic acid. The humic acid water-soluble fertilizer prepared by the invention realizes slow release and efficient utilization of nutrients through formation of physical and chemical barriers, chemical chelation, biological promotion and stable volatile components. The slow-release mechanism of the multi-component synergistic effect not only improves the utilization rate of the fertilizer, but also reduces the waste of nutrients and the pollution to the environment.

The invention discloses a dipotassium phosphate synergist for plant-based food and a preparation method of the dipotassium phosphate synergist. The dipotassium phosphate synergist for the plant-based food comprises the following components in percentage by mass: 15%-40% of nanocrystallized dipotassium phosphate; 30%-60% of composite plant colloid, wherein the composite plant colloid is prepared by cross-linking natural anionic polysaccharide and nonionic polysaccharide; 3%-8% of an interface function auxiliary agent, wherein the interface function auxiliary agent is prepared by adopting a layered film, nano-scale filling particles and molecular-scale chelating metal ions in a synergistic manner; 10%-25% of a microporous carrier; and 0.5%-3% of an ion migration inhibitor, which is prepared by synergistically using amphiphilic molecules and cationic polypeptides. The dipotassium phosphate synergistic system with the intelligent response characteristic is constructed through three technical innovations of nanostructure design, biological interface engineering and supercritical processing, and the problems of water separation, ion migration and texture stability of dipotassium phosphate in a plant protein matrix can be solved through a multi-component synergistic effect.

This invention discloses a preparation method and application of a lauryl bisphosphonate / mesoporous hydroxyapatite organic-inorganic hybrid composite material, belonging to the field of landfill leachate treatment technology. A solution of dipotassium hydrogen phosphate is prepared and then added dropwise to a mixed solution of calcium chloride and lauryl bisphosphonate. The pH of the solution is adjusted with alkali, washed with water, and then heated and dried to obtain the lauryl bisphosphonate / mesoporous hydroxyapatite organic-inorganic hybrid composite material. This composite material exhibits strong complexing ability with various heavy metal ions, and its adsorption performance is minimally affected by soluble organic acids in landfill leachate, ensuring long-term locking of heavy metal ions and avoiding secondary pollution caused by the re-dissolution of heavy metals. It has strong market potential and application value in the fields of landfill leachate treatment and environmental remediation.

The invention relates to a microbial activator for a high-wax-content oil reservoir and application of the microbial activator, and belongs to the technical field of oil exploitation. The activating agent comprises the following components: glucose, sodium nitrate, sodium chloride, magnesium sulfate, yeast extract, dipotassium phosphate, monopotassium phosphate and trace elements; the activator can be injected into a producing well to activate microorganisms in the producing well, and the activated microorganisms can act with crude oil, so that the wax content and the freezing point of the high-wax-content crude oil are reduced, the fluidity of the crude oil is enhanced, the yield of the oil well is improved, the lifting difficulty is reduced, and the crude oil is easy to flow out of a shaft. The activator has the characteristics of low corrosivity, no secondary pollution, low cost and the like, can be used for improving the single well yield of the high wax content reservoir, and meets the requirements of oil field production.

The invention discloses preparation and application of bacillus licheniformis for decomposing pigeon feathers, and relates to the field of environmental resource application in the breeding industry, and the preparation method comprises the following steps: step 1, strain activation: firstly resuscitating a freeze-dried strain, and then culturing and activating by adopting a gradient activation mode; 2, preparing a seed solution, namely culturing the strain activated in the step 1 in a seed culture medium to obtain the seed solution; according to the method, the components of the pre-activation culture medium comprise 1% of corn flour, 0.5% of soybean meal and 0.1% of dipotassium phosphate, the production cost is low, and the preparation feasibility and economic benefits of the bacillus licheniformis are improved; the feather decomposition efficiency is improved by 2-3 times through substrate pretreatment, the residual feather amount is reduced, meanwhile, the fermentation period is shortened to 40-50 hours, and the feather decomposition efficiency of the pigeons is improved; in the fermentation tank, the activity of the keratinase can reach 600U / mL, so that the decomposition efficiency can be improved, subsequent reuse is facilitated, and the utilization rate is increased.

The application discloses a Pediococcus pentosaceus with the functions of lowering cholesterol and inhibiting bacteria and a high-density industrial production fermentation medium of the Pediococcus pentosaceus, the Pediococcus pentosaceus is Pediococcus pentosaceus PG-2, and the preservation number is GDMCC No: 62392.The high-density industrial fermentation medium of the Pediococcus pentosaceus PG-2 contains 40 g / L of sugarcane molasses liquid, 20 g / L of corn syrup dry powder, 1.5 g / L of dipotassium hydrogen phosphate, 5 g / L of sodium acetate, 1 mL / L of Tween 80, 2 g / L of triammonium citrate, 0.2 g / L of magnesium sulfate heptahydrate, 0.05 g / L of manganese sulfate tetrahydrate, and the rest is water.The Pediococcus pentosaceus is cultured by using the medium, the bacterial concentration is significantly higher than that of MRS medium, a high-density bacterial body amount is effectively obtained, the utilization rate of industrial by-products is improved, the production cost is greatly reduced, and the production efficiency is improved.

The invention relates to the technical field of animal husbandry, in particular to a multipurpose animal feed conditioner and a using method thereof, and the conditioner is prepared from the following components in percentage by weight: 0.05%-0.1% of bacillus, 0.05%-0.1% of clostridium butyricum, 0.1%-0.2% of saccharomycetes, 0.05%-0.1% of lactobacillus acidophilus, 2.0% of glucose, 0.15% of yeast extract, 0.1%-0.15% of dipotassium phosphate, 0.05% of magnesium sulfate, 0.1% of sodium chloride and the balance of water. Through the synergistic effect of multiple bacteria, the feed utilization rate is remarkably increased, the animal growth is promoted, the immunity is enhanced, the use of antibiotics is reduced, the environmental pollution is reduced, and the synergistic development of economic benefits, ecological benefits and social benefits of livestock and poultry breeding is realized.

The present invention discloses a composite calcium carbonate powder for rubber and its preparation method, which comprises the following steps: Dissolve dipotassium hydrogen phosphate and mix it with a modified nano calcium carbonate coating body for activation, then mix it with mercapto chitosan in deionized water and react under alkaline conditions to obtain a composite nano calcium carbonate coating body; Disperse the composite nano calcium carbonate coating body and mix it with a mixed solution of an aqueous solution of methacrylic acid and an aqueous solution of sodium hydroxide, and then react with an aqueous solution of aluminum chloride to obtain a composite calcium carbonate powder; By compounding silica, carboxymethyl cellulose and mercapto chitosan on the nano calcium carbonate matrix, the present invention reduces the agglomeration of nano calcium carbonate, improves the reaction grafting ability of the nano calcium carbonate after being coated with silica, and forms a composite calcium carbonate powder with rich surface groups through a layer-by-layer coating method, which improves the crosslinking density of the composite calcium carbonate powder and the rubber material, has a high dispersion degree in rubber, and has a good improvement on the mechanical properties of rubber.

The invention provides a silage corn slow-release fertilizer and a preparation method thereof, and belongs to the technical field of slow-release fertilizers. The method comprises the following steps: mixing zirconocene dichloride and ethynyl biphenyl-3, 3 ', 5, 5'-tetracarboxylic acid, adding N, N-dimethylacetamide and water, reacting at 50-80 DEG C for 15-20 hours, cooling to room temperature, and washing with N, N-dimethylacetamide to obtain a porous carrier; mixing the porous carrier with dipotassium phosphate, ammonium chloride and water, and dispersing to obtain dispersion liquid; adding agricultural wastes into the dispersion liquid, uniformly stirring, slowly dropwise adding ammonium persulfate, and adding a monomer and a cross-linking agent to obtain a mixed solution; reacting for 1-2 hours at 60-80 DEG C in an inert atmosphere to obtain a semi-finished product; and washing and drying the obtained semi-finished product to obtain the silage corn slow-release fertilizer. The slow-release fertilizer provided by the invention can improve the water retention capacity of soil and prolong the nutrient release time, and is suitable for sandy soil or arid and semi-arid regions.

The invention discloses a phosphorus-modified calcium-based biomineral material for inhibiting leaching of heavy metals in fly ash and application, and belongs to the technical field of fly ash treatment. The material takes waste oyster shells as a raw material, and is prepared by grinding, sieving, dipotassium phosphate modification, pyrolysis at 400-600 DEG C, grinding and sieving with a 0.15-0.22 mm sieve, the pH is 10.2-11.3, the calcium accounts for 20-30%, and the phosphorus accounts for 6-8%. During application, the mass ratio of the material to fly ash is 0.01-0.1, and the material is cured for 24 hours and dried. The material can efficiently fix seven heavy metals such as As and Cd in the fly ash, the leaching concentration reaches the standard, the secondary release rate is low under the acidic condition, resource utilization of the oyster shells is achieved, the cost is low, and the material is suitable for stabilizing treatment of the waste incineration fly ash.

The invention provides high-sweetness modified milk and a preparation method thereof. The high-sweetness modified milk comprises the following raw materials in percentage by mass: 72%-80% of raw milk, 4%-5% of a sweetening agent, 0.05%-0.15% of dipotassium phosphate, 0.03%-0.1% of sodium bicarbonate, 0.001%-0.03% of beta-carotene and the balance of water and / or food auxiliary agents. The modified milk has the advantages of being low in sugar content and high in sweetness, and various health hidden dangers caused by the fact that a large amount of sugar or sugar substitutes are added to improve the sweetness are avoided.

Tartaric acid is a kind of organic acid existing in natural fruits or yoghurt, and is also named alpha-hydroxy acid. The pigment is a raw material endowed with a certain color and can attract human vision. The preparation method comprises the following steps: dissolving chitosan in citric acid, malic acid or tartaric acid in tartaric acid to prepare a chitosan solution; adding a ferrous sulfate solution into the solution, dialyzing, and adding disodium hydrogen phosphate or dipotassium phosphate to obtain the yellow pigment. The yellow pigment is bright in color and luster, good in water solubility and relatively stable in weak-acid and weak-base environments. After being placed, the color is not obviously changed. The color and luster are slightly different according to different added tartaric acid.

The invention discloses a method for detecting pesticide residues in a fat-soluble plant extract, which comprises the following steps: step 1, weighing a fat-soluble plant extract sample A which is uniformly mixed, adding water, acetonitrile, anhydrous magnesium sulfate, potassium chloride, monopotassium phosphate and dipotassium phosphate, oscillating and extracting, centrifuging, and taking supernate B for solvent replacement to obtain a sample solution C; 2, purifying the sample solution C through a composite solid-phase extraction column to obtain a purified solution D; the filler of the composite solid-phase extraction column comprises Z-Sep, florisil, neutral aluminum oxide, PSA and C18 from top to bottom; and step 3, carrying out solvent replacement on the purified solution D to obtain a sample solution E, and detecting the pesticide residues by using a gas chromatography-tandem mass spectrometry. By optimizing a sample pretreatment and purification process, accurate detection of the pesticide residues in the fat-soluble plant extract is realized.

The present invention relates to the technical field of microbial fermentation, and discloses a method for producing selenium-rich protein feed by multi-strain co-fermentation of Hami melons. The Hami melons are crushed, then mixed with a carbon source and a nitrogen source, and then inoculated with a fermentation agent for fermentation; the fermented product is dried and pulverized to obtain a protein feed; the fermentation agent includes Candida utilis, Aspergillus niger, and Bacillus licheniformis; the components of the Candida utilis culture medium include yeast extract 9.56 g / L, glucose 19.86 g / L, dipotassium hydrogen phosphate 1.45 g / L, magnesium sulfate 1.12 g / L, sodium selenite 25 mg / L, L-lysine and vitamin B1. In terms of mass concentration, the ratio of sodium selenite, L-lysine and vitamin B1 is 1:(116-232):(0.06-0.12). In the present invention, multi-strain co-fermentation of Hami melons is adopted to obtain a selenium-rich protein feed.

The invention relates to the technical field of fertilizers, and particularly discloses a liquid fertilizer containing bacillus velezensis LLH-D2. The organic fertilizer is prepared from the following raw materials in parts by weight: 24 to 26 parts of compound microorganism bacterium powder, 10 to 13 parts of chicken manure, 5 to 6 parts of dipotassium phosphate, 13 to 15 parts of glucose, 10 to 12 parts of amino acid, 16 to 18 parts of straw compound powder, 3 to 5 parts of sodium lignin sulfonate, 10 to 14 parts of attapulgite powder and 240 to 250 parts of water. According to the prepared liquid fertilizer, various raw materials are matched to jointly promote formation of a soil aggregate structure, increase the content of organic matters in soil, improve the micro-ecological environment of the soil and increase the yield of crops.

The invention relates to the technical field of material management, in particular to a dipotassium phosphate storage and distribution management system, which comprises an unbalance loading feature extraction module, a steering section risk identification module, a station passing feature classification module, a path stability evaluation module and a path combination optimization module. According to the method, dynamic extraction and normalization expression are carried out on material mass distribution and gravity center shift states in the loading process, and a path whole-course passing trend sequence is constructed in combination with steering response change in a discharging path and a discharging tail end passing deviation trend; then, traffic state judgment and path traffic grade evaluation are carried out through trend consistency and fluctuation characteristics, and path optimization is completed in a path combination stage based on trend abnormal section replacement and a trend sequence combination evaluation value; dynamic classification, trend identification and path selection optimization can be carried out on the passing risk state of the whole process from loading to unloading of dipotassium phosphate under the multi-station and multi-path condition.

The invention relates to the technical field of microbial culture media, in particular to a lactobacillus paracasei multifunctional culture medium and a preparation method and application thereof, and the lactobacillus paracasei multifunctional culture medium is prepared from the following components: spirulina platensis lysate, corn steep liquor, trehalose, glucose, Tween 80, dipotassium phosphate, sodium acetate, triammonium citrate, magnesium sulfate and manganese sulfate. The spirulina platensis lysate is prepared by lysing a spirulina platensis solution with the concentration of 110 g / L to 200 g / L; the pH value of the culture medium is 6.3 to 6.5; wherein 0.2 g to 0.8 g of corn steep liquor, 0.4 g to 1.6 g of trehalose, 5 g to 35 g of glucose, 0.4 ml to 1.6 ml of Tween 80, 0.5 g to 3.5 g of dipotassium phosphate, 2 g to 8 g of sodium acetate, 0.5 g to 3.5 g of triammonium citrate, 0.05 g to 0.35 g of magnesium sulfate and 0.02 g to 0.08 g of manganese sulfate are added into each liter of spirulina platensis lysate. According to the culture medium disclosed by the invention, rapid, high-density, high-activity, high-probiotic necessary characteristic and high-extracellular polysaccharide / extracellular protein yield culture of the lactobacillus paracasei in a batch culture mode is realized, and a foundation is laid for industrial application of the lactobacillus paracasei probiotics and the extracellular polysaccharide / extracellular protein.