152 results about "Dipotassium phosphate" patented technology

A forest fire retardant composition contains a retardant compound that includes a phosphate salt. The phosphate salt may include diammonium phosphate, diammonium orthophosphate, monoammonium phosphate, monoammonium orthophosphate, monosodium phosphate, disodium phosphate, disodium phosphate hydrate, sodium ammonium phosphate, sodium ammonium phosphate hydrate, sodium tripolyphosphate, trisodium phosphate, or dipotassium phosphate, and combinations thereof. The forest fire retardant composition may include an ammonium source. The composition may be in the form of a dry concentrate, a liquid concentrate, or a final diluted product. The final diluted product is effective in suppressing, retarding, and controlling forest fires while exhibiting corrosion resistance and low toxicity.

A forest fire retardant composition contains a retardant compound that includes a phosphate salt. The phosphate salt may include diammonium phosphate, diammonium orthophosphate, monoammonium phosphate, monoammonium orthophosphate, monosodium phosphate, disodium phosphate, disodium phosphate hydrate, sodium ammonium phosphate, sodium ammonium phosphate hydrate, sodium tripolyphosphate, trisodium phosphate, or dipotassium phosphate, and combinations thereof. The forest fire retardant composition may include an ammonium source. The composition may be in the form of a dry concentrate, a liquid concentrate, or a final diluted product. The final diluted product is effective in suppressing, retarding, and controlling forest fires while exhibiting corrosion resistance and low toxicity.

The invention discloses a humic acid liquid fertilizer and a preparation method and application thereof, and belongs to the technical field of liquid fertilizers, organic waste and ferric oxide are ball-milled and mixed, transferred into a muffle furnace, roasted for 2-2.5 h at 280-310 DEG C under nitrogen protection, transferred into a reaction kettle and stirred and mixed with a 10 wt% hydrogen peroxide aqueous solution and potassium hydroxide powder, and the humic acid liquid fertilizer is obtained. The preparation method comprises the following steps: adding potassium humate into a potassium humate solution, extracting for 40-60 minutes under the assistance of ultrasonic waves, filtering to obtain a potassium humate extract and filter residues, transferring the potassium humate extract into a stirring kettle, adding urea, dipotassium phosphate, a composite carbon dot lubricant and a suspension stabilizer into the stirring kettle, and uniformly mixing to obtain the humic acid liquid fertilizer. The suspension stabilizer can reduce the generation of flocculation and crystallization in the liquid fertilizer, and through the antifriction and lubrication effects of the composite carbon dot lubricant, the friction force between indissolvable solids and water outlet holes of the drip irrigation equipment is reduced, and the risk of blockage of the drip irrigation equipment is reduced.

The invention provides a culture medium formula capable of effectively improving vibrio inhibition capability of bacillus. The culture medium formula comprises 10-20 g of corn starch, 2-10 g of yeast powder, 2-10 g of malt extract, 5-20 g of glycerin, 0.5-1 g of L-aspartic acid, 1 g of dipotassium phosphate, 0.3 g of monopotassium phosphate, 0.5 g of magnesium sulfate, 1 g of calcium carbonate and 1000 mL of distilled water. When the culture medium is used for culturing bacillus capable of naturally inhibiting vibrio, the obtained culture has a better vibrio inhibiting effect.

The invention discloses an electrotransfection buffer solution and application of the electrotransfection buffer solution in exosome loaded polypeptide, and relates to the technical field of biology. The electrotransfection buffer solution is prepared from the following components: 80 to 120 mM of trehalose, 6 to 10 mM of dipotassium phosphate, 12 to 16 percent of iodixanol and a buffer solution. The invention also provides a method for carrying out exosome drug loading by using the electrotransfection buffer solution, more efficient loading of palmitoyl pentapeptide-4 by the exosome is realized, the loading capacity of molecules is improved on the premise of not losing the exosome, a higher collagen expression level can be obtained, and the method disclosed by the invention can be applied to large-scale and large-scale production. And the subsequent yield requirement is met.

The invention relates to the technical field of production of dipotassium phosphate, discloses a process method for continuously producing food-grade anhydrous dipotassium phosphate, aims to obtain a food-grade anhydrous dipotassium phosphate product through a drying reaction in a rotary polymerization furnace by a one-step method, and effectively solves the problems of multiple control procedures, complicated process control and high production cost. The coke content is greatly reduced under the condition of ensuring the dipotassium phosphate content.

The invention relates to the technical field of degreasing agents, in particular to a high-efficiency energy-saving degreasing agent and a preparation method thereof, and the high-efficiency energy-saving degreasing agent comprises the following components in parts by weight: 6-9 parts of recombinant thermostable lipase; 4-7 parts of alkaline protease; 1.5 to 2.5 parts of nano silver particles wrapped by chitosan; 0.8 to 1.8 parts of beta-cyclodextrin modified carbon quantum dots; 12 to 18 parts of sodium alginate based intelligent hydrogel microspheres; 18 to 22 parts of lauryl glucoside; 12 to 18 parts of coconut oil-based betaine; 6 to 9 parts of propylene glycol; 4 to 7 parts of d-limonene; 10 to 14 parts of polyethylene glycol 400; 2 to 4 parts of dipotassium phosphate; according to the modified recombinant thermostable lipase, the low-temperature activity and the pH adaptability are remarkably improved while the high catalytic efficiency is maintained, and the antibacterial performance is enhanced by combining the nano-silver particles wrapped by the chitosan with the beta-cyclodextrin modified carbon quantum dots.

The invention relates to the technical field of microorganisms, and discloses an induction method of VBNC state shigella, a resuscitation medium and application, and the resuscitation medium comprises Rpf recombinant protein, MgSO4, sodium pyruvate, vitamin B12, tryptone, glucose, dipotassium phosphate, monopotassium phosphate, sodium chloride and Tween 80. The resuscitation culture medium obtained by the invention can be used for successfully culturing Shigella in a VBNC state.

The invention discloses a dental whitening gel and a preparation method thereof, and belongs to the technical field of oral medicine. Comprising the following components in percentage by mass: 10%-20% of urea peroxide, 0.5%-1% of sodium alginate, 1%-2% of tea tree oil, 2%-3% of dipotassium phosphate, 2%-4% of monopotassium phosphate, 3%-5% of carbomer, 3%-5% of glycerol, 59%-78% of deionized water and 0.5%-1% of pentasodium diethylenetriamine pentaacetate. The oral cavity cleaning agent has high safety, and does not have serious toxicity and irritation to the oral cavity.

The invention provides a method for producing succinic acid by immobilized cells, and belongs to the technical field of fermentation. The fermentation culture medium comprises the following components: 50 to 54 g / L of glucose, 8 to 10 g / L of corn steep liquor, 5.7 to 5.9 g / L of monopotassium phosphate, 6.3 to 6.5 g / L of dipotassium phosphate, 0.17 to 0.22 mg / L of calcium chloride, 1.67 to 1.73 mg / L of cobalt chloride hexahydrate, 0.46 to 0.50 mg / L of zinc sulfate heptahydrate, 1.6 to 1.8 mg / L of biotin, 6.0 to 6.5 mg / L of nicotinamide, 8.2 to 8.5 mg / L of vitamin B5 and 0.32 to 0.34 g / L of an active agent. By adopting the method disclosed by the invention, the succinic acid production rate is high, the malic acid content is low, the immobilized microspheres can be repeatedly fermented for multiple times, and the succinic acid content of a single batch is stable and is always kept at a relatively high level.

The invention discloses a method for determining impurities in a desloratadine oral solution, the impurities comprise a desloratadine impurity 1, the determination method adopts a high performance liquid chromatography, and the detection conditions of the high performance liquid chromatography are as follows: a chromatographic column is an octyl silane bonded silica gel column or an efficiency equivalent column; the detection wavelength is 254 + / -2nm; the detector is an ultraviolet detector; the column temperature is 35-45 DEG C; the flow rate is 1.1 to 1.3 mL / min; mobile phases: a dipotassium phosphate buffer solution, methanol and acetonitrile; the elution mode is isocratic elution. The determination method disclosed by the invention is good in specificity, good in separation degree, high in sensitivity and good in accuracy, can fully meet the requirements of impurity determination, has important meanings for evaluating the quality of raw material medicines and the advantages and disadvantages of a preparation process, controlling the quality of a final product and detecting a stable sample, and is high in practicability in actual quality control work.

The present invention belongs to the technical field of rapid detection of drug sensitivity for bacteria. Disclosed are an enrichment culture medium, and a preparation method therefor and the use thereof. The enrichment culture medium comprises the following raw materials in parts by mass: 15.0-20.0 parts of tryptone, 3.5-5.5 parts of peptone, 1.5-3.5 parts of glucose, 5.0-6.5 parts of yeast extract, 2.0-2.5 parts of dipotassium phosphate, 2.0-3.5 parts of disodium hydrogen phosphate, 1.0-1.5 parts of monopotassium phosphate, 2.5-7.5 parts of sodium chloride, 12.0-18.0 parts of proteose peptone, 15.0-22.0 parts of beef heart infusion powder, 8.0-12.0 parts of soya peptone, 1.0-5.0 parts of 0.1% nicotinamide adenine dinucleotide (NAD), 30-100 parts of newborn calf serum inactivated at 56°C for 30 min, 2.0-4.0 parts of mannitol, 3.0-5.0 parts of sodium pyruvate, 0.23-0.45 parts of cycloheximide, 0.8-2.0 parts of 0.05% bromothymol blue, 2.5-4.5 parts of 0.1% methylene blue, and 1000 parts of distilled water, with the pH value of 6.8-7.5. The enrichment culture medium is used to screen effective therapeutic drugs for bacterial diseases in poultry, and is convenient to use and has a single enrichment effect superior to that of respective selective enrichment broths of target bacteria. Therefore, the culture medium can realize rapid proliferation of common pathogenic bacteria in poultry.

The present invention discloses a cattle fattening and growth promoting compound preparation and a preparation method thereof, and particularly relates to the technical field of livestock breeding, the cattle fattening and growth promoting compound preparation comprises 30-40 parts of rhizoma atractylodis, 30-40 parts of radix astragali, 10-20 parts of cortex magnoliae officinalis, 10-20 parts of jujube, 5-15 parts of coated guanidinoacetic acid, 3-5 parts of a compound probiotic liquid, soybean meal accounting for 2% of the total mass of the compound preparation, corn flour accounting for 1% of the total mass of the compound preparation, and the balance of water. The molasses accounts for 1% of the total mass of the compound preparation, and the dipotassium phosphate accounts for 0.5% of the total mass of the compound preparation. The preparation method comprises the following steps: S1, pretreating the traditional Chinese medicines; S2, mixing the raw materials; S3, adding bacterial liquid and enveloped guanidinoacetic acid; and S4, fermenting. According to the compound preparation, the growth speed of cattle and the feed conversion rate can be remarkably increased, the immunity of the cattle is enhanced, the health of the cattle is guaranteed, meanwhile, green and safe breeding is achieved, in addition, the preparation technology is simple and easy to operate, and the quality and stability of the preparation can be guaranteed.

The present invention provides low glycemic index (GI) total nutrition formula emulsion and a preparation method therefor. The low GI total nutrition formula emulsion comprises the following raw material components in parts by weight: 10-14 parts of carbohydrates, 4-5 parts of calcium caseinate, 1.3-2 parts of dietary fiber, 0.1-0.15 part of sodium chloride, 0.1-0.12 part of sodium citrate, 0.1-0.15 part of potassium citrate, 0.15-0.2 part of magnesium chloride, 0.1-0.15 part of dipotassium hydrogen phosphate, 0.05-0.1 part of compound vitamins, 0.05-0.1 part of compound minerals, 2.5-3.6 parts of compound oil, 0.3-0.5 part of phospholipid, 0.2-0.5 part of medium chain triglyceride (MCT), 0.04-0.08 part of colloids. The preparation method comprises: preparing aqueous phases and oil phases from the raw materials step by step; mixing the aqueous phases and oil phases; and shearing, homogenizing and sterilizing the mixture to obtain the low GI total nutrition emulsion. The product features balanced and complete nutrition.

The invention provides a butyric acid probiotic culture medium which comprises the following components: 6-12 g / L of peptone, 6-12 g / L of beef extract, 1-5 g / L of yeast extract, 4-8 g / L of glucose, 4-8 g / L of sodium chloride, 0.5-2 g / L of soluble starch, 1-5 g / L of sodium acetate, 0.1-1.0 g / L of L-cysteine hydrochloride, 4-8 g / L of xylan, 1-3 g / L of dipotassium phosphate, 0.1-1.0 g / L of magnesium sulfate heptahydrate and 0.01-0.05 g / L of ferrous sulfate. The invention specifically provides a method for culturing a lactobacillus plantarum and clostridium tyrobutyricum high-yield butyric acid collaborative system by using the culture medium, and the method not only improves the butyric acid yield, but also reduces the production cost of butyric acid biotransformation, and has remarkable economic benefits.

The invention discloses a haemophilus parasuis high-density fermentation culture medium as well as a culture method and application thereof, and relates to the technical field of microbial fermentation. The haemophilus parasuis high-density fermentation culture medium is prepared from the following components according to the content in 1L: 1.8 to 2g / L of glucose, 20 to 25g / L of organic nitrogen source, 2.0 to 3.0 g / L of dipotassium phosphate, 4.5 to 5.5 g / L of sodium chloride, 0.5 to 1.8 mg / L of NAD (Nicotinamide Adenine Dinucleotide), 8.2 to 8.6 mL / L of fetal calf serum and the balance of water, and the pH value is 7.0 to 7.5; wherein the organic nitrogen source is composed of Oxoid yeast and green source soybean peptone in a mass ratio of 1: 2. By optimizing the proportion of the glucose, the organic nitrogen source and the fetal calf serum, the viable count of the haemophilus parasuis is remarkably increased, when the culture medium is used for fermentation culture, OD600 can reach 1.8 through 7-hour fermentation, the viable count reaches 1010 CFU / mL, the viable count is increased by more than one time compared with that of a traditional culture medium, and the culture medium is suitable for large-scale production. And a high-density thallus raw material is provided for vaccine production.

The group B streptococcus fermentation culture medium comprises salt substances, a carbon source, an amino acid source, a vitamin source, a purine source, a pyrimidine source, a nitrogen source and solvent water, salt substances: 0.2 g / L to 0.5 g / L of dipotassium phosphate, 0.3 g / L to 0.5 g / L of monopotassium phosphate, 3 g / L to 5 g / L of disodium hydrogen phosphate, 2 g / L to 5 g / L of sodium dihydrogen phosphate monohydrate, 0.5 g / L to 0.8 g / L of ammonium sulfate, 0.2 g / L to 0.5 g / L of magnesium sulfate heptahydrate, 0.01 g / L to 0.03 g / L of manganous sulfate monohydrate and 0.03 g / L to 0.06 g / L of ferrous sulfate; an amino acid source: 0.2 g / L to 0.5 g / L of glycine and 0.1 g / L to 0.3 g / L of tryptophan; a nitrogen source: 3-6g / L of acid hydrolyzed casein; the feed also comprises 5-10g / L of yeast extract powder. The group B streptococcus is fermented and cultured by using the fermentation culture medium disclosed by the invention, sufficient nutrient substances can be provided for strains, and the bacterium concentration is effectively improved.

The invention relates to the technical field of pathogenic microorganism detection, and discloses a helicobacter pylori detection kit based on a urease method and a preparation method, the helicobacter pylori detection kit comprises a detection sleeve card, detection test paper and a tartar sampling label, the test paper is soaked in a reaction solution and a buffer solution in the preparation process of the detection test paper, the pH of the buffer solution is 6.5-7.0, and the tartar sampling label is used for sampling tartar. The buffer solution comprises monopotassium phosphate and dipotassium phosphate, the final concentration of the monopotassium phosphate is 14-30 g / L, and the final concentration of the dipotassium phosphate is 1.8-3.6 g / L. The method can solve the problems of high false positive rate of detection results, poor accuracy, slow reagent response speed and the like caused by complex oral environment (such as diet residue and non-specific bacterial interference) and lack of an effective control system when a tartar sample is used for detecting helicobacter pylori in the prior art.

The invention provides a method for rapidly determining the vitality of blueberry pollen, and relates to the technical field of blueberry pollen vitality detection. The method for rapidly determining the vitality of the blueberry pollen comprises the following specific steps: step 1, pollen collection: when blueberry flowers are in a big flower bud stage, picking the flowers into a self-sealing bag, putting the self-sealing bag into a preservation box with ice cubes, taking the preservation box back to a laboratory, and performing detection on the day; and step 2, preparing a PBS solution, namely fixing the volume of 3.16 g of monopotassium phosphate to 100 ml, fixing the volume of 2.28 g of dipotassium phosphate to 100 ml, preparing a mixed solution, and adding an acidic titration solution or an alkaline titration solution to prepare the PBS solution with the pH value of 7.0. TTC (2, 3, 5-triphenyltetrazole chloride) is dissolved in a PBS solution, a 1% TTC solution is prepared for dyeing, the blueberry pollen suspension prepared by the method can fully absorb a reaction solution to avoid pollen viability errors caused by individual differences, meanwhile, the blueberry pollen dyeing time can be 20 min, and the blueberry pollen viability detection time is greatly shortened.

The invention relates to the technical field of tooth restoration, in particular to a composition for repairing tooth leukoplakia and a preparation method of the composition. The composition for repairing the tooth leukoplakia comprises the following components: artificial saliva, and the artificial saliva comprises 0.2 g / L to 1 g / L of dipotassium phosphate and 0.2 g / L to 1 g / L of calcium chloride. Furthermore, the composition also comprises an active glass composition. By adopting the technical scheme, the artificial saliva containing phosphate radicals, potassium ions and calcium ions with specific concentrations can be matched with permeable resin, an active glass composition and the like to achieve a good tooth leukoplakia repairing effect, so that the technical problem that the application of permeable resin is limited in the prior art is solved.

The invention discloses a bone regeneration multiple bionic scaffold material and a preparation method thereof, and relates to the technical field of bionic bones. According to the bone regeneration multi-bionic scaffold material and the preparation method thereof, a polylactic acid block copolymer is used as a raw material, a 3D printing technology is adopted to prepare a substrate scaffold meeting the bone defect morphology, and then the substrate scaffold is alternately soaked in a collagen mixed solution and a modified layered dihydroxide solution for multiple times to obtain the bone regeneration multi-bionic scaffold material. A bionic matrix layer composed of collagen / layered dihydroxide is formed on the surface of the base stent, and finally, the base stent with the bionic matrix layer attached to the surface is alternately soaked in dipotassium phosphate and calcium chloride solutions for multiple times in sequence; and performing in-situ mineralization on the substrate support with the bionic matrix layer attached on the surface to form calcium phosphate nanoparticles, and finally cleaning and drying to obtain the calcium phosphate nano-particles. The bone regeneration multi-bionic scaffold material prepared by the preparation method disclosed by the invention is relatively good in biocompatibility and relatively good in mechanical property.

The present invention relates to the technical field of centrifugal dehydration, and specifically, to a centrifugal dehydration device for producing slurry for dipotassium hydrogen phosphate. The device comprises a bracket, a barrel is fixedly connected inside the bracket, and a barrel cover is movably connected to the top of the barrel. The slurry inside the separation component is stirred by a centrifugal component, so that the slurry rotates at high speed to generate centrifugal force, so that the liquid in the slurry is filtered and removed through the separation component, and the slurry sticking to the inner wall of the separation component is scraped off during the rotation of the centrifugal component, and the scraped slurry will be subjected to secondary centrifugal stirring and dehydration inside the separation component. According to the different concentrations of the slurry, the mesh size of the separation component is adjusted to adapt to the centrifugal dehydration of slurries of different concentrations, and while the mesh size is adjusted, the stirring angle of the blades inside the centrifugal component is adjusted, so as to adjust the stirring flexibility of the centrifugal component, so as to facilitate adaptation to different centrifugal stirring states of the slurry.

The invention relates to a humic acid water-soluble fertilizer as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The humic acid water-soluble fertilizer comprises a base fertilizer raw material and a thin film layer, wherein the base fertilizer raw material comprises the following components: humate, urea, dipotassium phosphate, potassium sulfate, calcium nitrate, magnesium sulfate, sodium dodecyl benzene sulfonate, carboxymethyl cellulose sodium and propylene glycol; the raw material of the thin film layer is a mixture of alginate oligosaccharide, cinnamon oil and amino acid; the amino acid mixture consists of phenylalanine and glutamic acid. The humic acid water-soluble fertilizer prepared by the invention realizes slow release and efficient utilization of nutrients through formation of physical and chemical barriers, chemical chelation, biological promotion and stable volatile components. The slow-release mechanism of the multi-component synergistic effect not only improves the utilization rate of the fertilizer, but also reduces the waste of nutrients and the pollution to the environment.

The invention discloses a dipotassium phosphate synergist for plant-based food and a preparation method of the dipotassium phosphate synergist. The dipotassium phosphate synergist for the plant-based food comprises the following components in percentage by mass: 15%-40% of nanocrystallized dipotassium phosphate; 30%-60% of composite plant colloid, wherein the composite plant colloid is prepared by cross-linking natural anionic polysaccharide and nonionic polysaccharide; 3%-8% of an interface function auxiliary agent, wherein the interface function auxiliary agent is prepared by adopting a layered film, nano-scale filling particles and molecular-scale chelating metal ions in a synergistic manner; 10%-25% of a microporous carrier; and 0.5%-3% of an ion migration inhibitor, which is prepared by synergistically using amphiphilic molecules and cationic polypeptides. The dipotassium phosphate synergistic system with the intelligent response characteristic is constructed through three technical innovations of nanostructure design, biological interface engineering and supercritical processing, and the problems of water separation, ion migration and texture stability of dipotassium phosphate in a plant protein matrix can be solved through a multi-component synergistic effect.

This invention discloses a preparation method and application of a lauryl bisphosphonate / mesoporous hydroxyapatite organic-inorganic hybrid composite material, belonging to the field of landfill leachate treatment technology. A solution of dipotassium hydrogen phosphate is prepared and then added dropwise to a mixed solution of calcium chloride and lauryl bisphosphonate. The pH of the solution is adjusted with alkali, washed with water, and then heated and dried to obtain the lauryl bisphosphonate / mesoporous hydroxyapatite organic-inorganic hybrid composite material. This composite material exhibits strong complexing ability with various heavy metal ions, and its adsorption performance is minimally affected by soluble organic acids in landfill leachate, ensuring long-term locking of heavy metal ions and avoiding secondary pollution caused by the re-dissolution of heavy metals. It has strong market potential and application value in the fields of landfill leachate treatment and environmental remediation.

The invention relates to a microbial activator for a high-wax-content oil reservoir and application of the microbial activator, and belongs to the technical field of oil exploitation. The activating agent comprises the following components: glucose, sodium nitrate, sodium chloride, magnesium sulfate, yeast extract, dipotassium phosphate, monopotassium phosphate and trace elements; the activator can be injected into a producing well to activate microorganisms in the producing well, and the activated microorganisms can act with crude oil, so that the wax content and the freezing point of the high-wax-content crude oil are reduced, the fluidity of the crude oil is enhanced, the yield of the oil well is improved, the lifting difficulty is reduced, and the crude oil is easy to flow out of a shaft. The activator has the characteristics of low corrosivity, no secondary pollution, low cost and the like, can be used for improving the single well yield of the high wax content reservoir, and meets the requirements of oil field production.

The invention discloses preparation and application of bacillus licheniformis for decomposing pigeon feathers, and relates to the field of environmental resource application in the breeding industry, and the preparation method comprises the following steps: step 1, strain activation: firstly resuscitating a freeze-dried strain, and then culturing and activating by adopting a gradient activation mode; 2, preparing a seed solution, namely culturing the strain activated in the step 1 in a seed culture medium to obtain the seed solution; according to the method, the components of the pre-activation culture medium comprise 1% of corn flour, 0.5% of soybean meal and 0.1% of dipotassium phosphate, the production cost is low, and the preparation feasibility and economic benefits of the bacillus licheniformis are improved; the feather decomposition efficiency is improved by 2-3 times through substrate pretreatment, the residual feather amount is reduced, meanwhile, the fermentation period is shortened to 40-50 hours, and the feather decomposition efficiency of the pigeons is improved; in the fermentation tank, the activity of the keratinase can reach 600U / mL, so that the decomposition efficiency can be improved, subsequent reuse is facilitated, and the utilization rate is increased.

The application discloses a Pediococcus pentosaceus with the functions of lowering cholesterol and inhibiting bacteria and a high-density industrial production fermentation medium of the Pediococcus pentosaceus, the Pediococcus pentosaceus is Pediococcus pentosaceus PG-2, and the preservation number is GDMCC No: 62392.The high-density industrial fermentation medium of the Pediococcus pentosaceus PG-2 contains 40 g / L of sugarcane molasses liquid, 20 g / L of corn syrup dry powder, 1.5 g / L of dipotassium hydrogen phosphate, 5 g / L of sodium acetate, 1 mL / L of Tween 80, 2 g / L of triammonium citrate, 0.2 g / L of magnesium sulfate heptahydrate, 0.05 g / L of manganese sulfate tetrahydrate, and the rest is water.The Pediococcus pentosaceus is cultured by using the medium, the bacterial concentration is significantly higher than that of MRS medium, a high-density bacterial body amount is effectively obtained, the utilization rate of industrial by-products is improved, the production cost is greatly reduced, and the production efficiency is improved.

The invention discloses a yeast culture medium as well as a fermentation culture method and application thereof, and belongs to the field of microbial fermentation. The saccharomycetes culture medium is prepared from the following raw materials in percentage by mass: 0.2 to 1 percent of wheat dry alcohol lees, 0.1 to 1 percent of yeast extract powder, 1 to 8 percent of molasses, 1 to 3 percent of methanol, 0.1 to 0.9 percent of disodium hydrogen phosphate, 0.1 to 1 percent of ammonium citrate, 0.1 to 1 percent of dipotassium phosphate, 0.005 to 0.05 percent of magnesium sulfate, 0.002 to 0.015 percent of manganese chloride and the balance of water. The culture medium is reasonable in formula, under the condition that glucose and peptone are not added and only a small amount of yeast extract powder is added, thallus growth can still be effectively promoted in the same fermentation period, and compared with a traditional saccharomycetes culture medium, the thallus density and the acid yield in a bacterial solution are remarkably improved, and the industrial production cost is remarkably reduced.