38 results about "Dipotassium phosphate" patented technology

The invention relates to the technical field of production of dipotassium phosphate, discloses a process method for continuously producing food-grade anhydrous dipotassium phosphate, aims to obtain a food-grade anhydrous dipotassium phosphate product through a drying reaction in a rotary polymerization furnace by a one-step method, and effectively solves the problems of multiple control procedures, complicated process control and high production cost. The coke content is greatly reduced under the condition of ensuring the dipotassium phosphate content.

The invention relates to the technical field of pathogenic microorganism detection, and discloses a helicobacter pylori detection kit based on a urease method and a preparation method, the helicobacter pylori detection kit comprises a detection sleeve card, detection test paper and a tartar sampling label, the test paper is soaked in a reaction solution and a buffer solution in the preparation process of the detection test paper, the pH of the buffer solution is 6.5-7.0, and the tartar sampling label is used for sampling tartar. The buffer solution comprises monopotassium phosphate and dipotassium phosphate, the final concentration of the monopotassium phosphate is 14-30 g / L, and the final concentration of the dipotassium phosphate is 1.8-3.6 g / L. The method can solve the problems of high false positive rate of detection results, poor accuracy, slow reagent response speed and the like caused by complex oral environment (such as diet residue and non-specific bacterial interference) and lack of an effective control system when a tartar sample is used for detecting helicobacter pylori in the prior art.

The invention provides a method for rapidly determining the vitality of blueberry pollen, and relates to the technical field of blueberry pollen vitality detection. The method for rapidly determining the vitality of the blueberry pollen comprises the following specific steps: step 1, pollen collection: when blueberry flowers are in a big flower bud stage, picking the flowers into a self-sealing bag, putting the self-sealing bag into a preservation box with ice cubes, taking the preservation box back to a laboratory, and performing detection on the day; and step 2, preparing a PBS solution, namely fixing the volume of 3.16 g of monopotassium phosphate to 100 ml, fixing the volume of 2.28 g of dipotassium phosphate to 100 ml, preparing a mixed solution, and adding an acidic titration solution or an alkaline titration solution to prepare the PBS solution with the pH value of 7.0. TTC (2, 3, 5-triphenyltetrazole chloride) is dissolved in a PBS solution, a 1% TTC solution is prepared for dyeing, the blueberry pollen suspension prepared by the method can fully absorb a reaction solution to avoid pollen viability errors caused by individual differences, meanwhile, the blueberry pollen dyeing time can be 20 min, and the blueberry pollen viability detection time is greatly shortened.

This invention discloses a preparation method and application of a lauryl bisphosphonate / mesoporous hydroxyapatite organic-inorganic hybrid composite material, belonging to the field of landfill leachate treatment technology. A solution of dipotassium hydrogen phosphate is prepared and then added dropwise to a mixed solution of calcium chloride and lauryl bisphosphonate. The pH of the solution is adjusted with alkali, washed with water, and then heated and dried to obtain the lauryl bisphosphonate / mesoporous hydroxyapatite organic-inorganic hybrid composite material. This composite material exhibits strong complexing ability with various heavy metal ions, and its adsorption performance is minimally affected by soluble organic acids in landfill leachate, ensuring long-term locking of heavy metal ions and avoiding secondary pollution caused by the re-dissolution of heavy metals. It has strong market potential and application value in the fields of landfill leachate treatment and environmental remediation.

The invention discloses preparation and application of bacillus licheniformis for decomposing pigeon feathers, and relates to the field of environmental resource application in the breeding industry, and the preparation method comprises the following steps: step 1, strain activation: firstly resuscitating a freeze-dried strain, and then culturing and activating by adopting a gradient activation mode; 2, preparing a seed solution, namely culturing the strain activated in the step 1 in a seed culture medium to obtain the seed solution; according to the method, the components of the pre-activation culture medium comprise 1% of corn flour, 0.5% of soybean meal and 0.1% of dipotassium phosphate, the production cost is low, and the preparation feasibility and economic benefits of the bacillus licheniformis are improved; the feather decomposition efficiency is improved by 2-3 times through substrate pretreatment, the residual feather amount is reduced, meanwhile, the fermentation period is shortened to 40-50 hours, and the feather decomposition efficiency of the pigeons is improved; in the fermentation tank, the activity of the keratinase can reach 600U / mL, so that the decomposition efficiency can be improved, subsequent reuse is facilitated, and the utilization rate is increased.

The invention discloses a phosphorus-modified calcium-based biomineral material for inhibiting leaching of heavy metals in fly ash and application, and belongs to the technical field of fly ash treatment. The material takes waste oyster shells as a raw material, and is prepared by grinding, sieving, dipotassium phosphate modification, pyrolysis at 400-600 DEG C, grinding and sieving with a 0.15-0.22 mm sieve, the pH is 10.2-11.3, the calcium accounts for 20-30%, and the phosphorus accounts for 6-8%. During application, the mass ratio of the material to fly ash is 0.01-0.1, and the material is cured for 24 hours and dried. The material can efficiently fix seven heavy metals such as As and Cd in the fly ash, the leaching concentration reaches the standard, the secondary release rate is low under the acidic condition, resource utilization of the oyster shells is achieved, the cost is low, and the material is suitable for stabilizing treatment of the waste incineration fly ash.

Tartaric acid is a kind of organic acid existing in natural fruits or yoghurt, and is also named alpha-hydroxy acid. The pigment is a raw material endowed with a certain color and can attract human vision. The preparation method comprises the following steps: dissolving chitosan in citric acid, malic acid or tartaric acid in tartaric acid to prepare a chitosan solution; adding a ferrous sulfate solution into the solution, dialyzing, and adding disodium hydrogen phosphate or dipotassium phosphate to obtain the yellow pigment. The yellow pigment is bright in color and luster, good in water solubility and relatively stable in weak-acid and weak-base environments. After being placed, the color is not obviously changed. The color and luster are slightly different according to different added tartaric acid.

The invention discloses a method for detecting pesticide residues in a fat-soluble plant extract, which comprises the following steps: step 1, weighing a fat-soluble plant extract sample A which is uniformly mixed, adding water, acetonitrile, anhydrous magnesium sulfate, potassium chloride, monopotassium phosphate and dipotassium phosphate, oscillating and extracting, centrifuging, and taking supernate B for solvent replacement to obtain a sample solution C; 2, purifying the sample solution C through a composite solid-phase extraction column to obtain a purified solution D; the filler of the composite solid-phase extraction column comprises Z-Sep, florisil, neutral aluminum oxide, PSA and C18 from top to bottom; and step 3, carrying out solvent replacement on the purified solution D to obtain a sample solution E, and detecting the pesticide residues by using a gas chromatography-tandem mass spectrometry. By optimizing a sample pretreatment and purification process, accurate detection of the pesticide residues in the fat-soluble plant extract is realized.

The invention relates to the technical field of material management, in particular to a dipotassium phosphate storage and distribution management system, which comprises an unbalance loading feature extraction module, a steering section risk identification module, a station passing feature classification module, a path stability evaluation module and a path combination optimization module. According to the method, dynamic extraction and normalization expression are carried out on material mass distribution and gravity center shift states in the loading process, and a path whole-course passing trend sequence is constructed in combination with steering response change in a discharging path and a discharging tail end passing deviation trend; then, traffic state judgment and path traffic grade evaluation are carried out through trend consistency and fluctuation characteristics, and path optimization is completed in a path combination stage based on trend abnormal section replacement and a trend sequence combination evaluation value; dynamic classification, trend identification and path selection optimization can be carried out on the passing risk state of the whole process from loading to unloading of dipotassium phosphate under the multi-station and multi-path condition.

The invention discloses an acetic acid inhibition culture medium and application thereof in lactic acid fermentation production. The acetic acid inhibition culture medium is prepared from 15-30 g / L of peptone, 5-10 g / L of yeast extract powder, 50-100 g / L of glucose, 1-2 mL / L of Tween, 1-2 g / L of ammonium citrate, 1-2 g / L of dipotassium phosphate, 0.2-0.4 g / L of magnesium sulfate, 0.1-0.2 g / L of ferrous sulfate, 0.1-0.2 g / L of copper sulfate, 0.05-0.2 g / L of manganese sulfate, 0.005-0.02 g / L of biotin and 0.005-0.02 g / L of riboflavin. The method has remarkable technical advantages in the aspects of inhibiting the generation of a byproduct acetic acid and improving the yield of lactic acid and the fermentation stability, can effectively overcome the defects in the prior art, and has relatively high economic value and popularization and application prospects.

The invention discloses a culture medium for promoting intestinal colonization of probiotics. Every 1000 parts by weight of water of the culture medium is prepared from the following components in parts by weight: 2 to 10 parts of casein, 4 to 12 parts of yeast extract powder, 10 to 12 parts of beef extract powder, 1.5 to 2.5 parts of trisodium citrate or triammonium citrate, 4.5 to 5.5 parts of sodium acetate, 0.1 to 0.3 part of magnesium sulfate heptahydrate, 0.05 to 0.1 part of manganese chloride and 1 to 3 parts of dipotassium phosphate. When the culture medium is used for culturing the probiotics, the secretion amount of exopolysaccharides, proteins and other substances can be remarkably increased, a protective layer is formed, the stress resistance of the strain is improved, the survival ability of the probiotics in a complex gastrointestinal tract environment is improved, and a new strategy is provided for development of the oral probiotics.

The invention discloses a method for separating tungsten and vanadium from a mixed solution containing tungsten and vanadium step by step, which comprises the following steps: S1, carrying out impurity removal pretreatment on the mixed solution containing tungsten and vanadium, adjusting the pH value to 7-9, and filtering to obtain a pre-exchange solution; s2, adsorbing the pre-exchange liquid through ion exchange resin until the resin is adsorbed to be saturated; s3, carrying out step-by-step desorption on the resin saturated by adsorption, firstly introducing an acidic desorption agent into the resin to desorb vanadium to obtain a vanadium desorption solution, washing the resin with water, and then introducing an alkaline desorption agent into the resin to desorb tungsten to obtain a tungsten desorption solution; the acidic desorption agent comprises citric acid and a monopotassium phosphate-dipotassium phosphate buffer solution; the alkaline desorption agent comprises an alkaline solution and an anionic surfactant; and S4, the vanadium desorption solution and the tungsten desorption solution are subjected to aftertreatment, and tungsten and vanadium products are obtained. According to the method for separating tungsten and vanadium, efficient separation of tungsten and vanadium can be achieved, and the comprehensive utilization rate of tungsten and vanadium from a mixed solution to a pure product is increased.

The invention relates to the technical field of microorganisms, and discloses an improved LST broth culture medium and application, and the improved LST broth culture medium comprises tryptone, ammonium chloride, lactose, dipotassium phosphate, monopotassium phosphate, sodium lauryl sulfate, Rpf recombinant protein, magnesium sulfate, sodium pyruvate and pyridoxal phosphate. The improved LST broth culture medium disclosed by the invention can be used for successfully enriching the VBNC-state escherichia coli, so that researchers can successfully detect the VBNC-state escherichia coli in a culture manner.

The invention discloses a pesticide synergist and a preparation method thereof. The synergist is prepared from 7-9 parts of potassium oleate, 1-2 parts of dipotassium phosphate and 1-2 parts of 2-hydroxy-4-methylvaleric acid according to a specific mass ratio. The preparation method comprises the following steps: stirring and mixing the components at normal temperature until the components are completely dissolved to form a homogeneous solution. The synergist disclosed by the invention can be compounded with chemical pesticides such as triadimefon, carbendazim and azoxystrobin for use, and by enhancing the attachment and permeation of the pesticides on the surfaces of plants and the binding efficiency of the pesticides and pathogenic bacteria targets, the control effect of 90% or above can be still maintained under the condition that the dosage of the chemical pesticides is reduced by 50%-80%, and pesticide residues and environmental risks are remarkably reduced. The invention has the advantages of easily available raw materials, simple process and low cost, is suitable for large-scale production, and has important significance for realizing agricultural pesticide reduction and synergism and sustainable development.

The invention relates to an inonotus obliquus fermentation product and application of the inonotus obliquus fermentation product in hypoglycemic drugs, and belongs to the technical field of inonotus obliquus fermentation products. A component culture medium is prepared from 10-15 parts of plant flour, 3-6 parts of glucose, 0.8-1.2 parts of dipotassium phosphate, 1-2 parts of magnesium sulfate, 5-8 parts of protein peptide, 4-8 parts of agar and 60-80 parts of purified water; the component B culture medium is prepared from the following raw materials in parts by weight: 12-15 parts of plant starch, 2-4 parts of glucose, 1-3 parts of oxytetracycline, 1-3 parts of chloramphenicol, 8-16 parts of sodium hyaluronate and 60-80 parts of purified water. Through the multi-target synergistic effect of improving insulin resistance, protecting islet cells, inhibiting sugar absorption and the like, the hypoglycemic effect is obvious, and the prepared inonotus obliquus fermentation product is simple in process, low in operation difficulty, suitable for industrial production and capable of being applied to the fields of cosmetics, textile industry and medicine.

The invention relates to the technical field of intestinal microecology, and particularly discloses an in-vitro fermentation system for rapid targeted screening, regulation and control of intestinal TMA generation, an establishment method and application, and the in-vitro fermentation system comprises the following components in parts by weight: 2-3 parts of yeast extract, 0.8-1.2 parts of L-cysteine, 1.5-2.5 parts of heme, 0.8-1.0 part of sodium chloride, 0.008-0.012 part of calcium chloride hexahydrate and 0.4-0.5 part of dipotassium phosphate. The feed is prepared from the following raw materials in parts by weight: 0.08 to 0.1 part of magnesium sulfate heptahydrate, 0.15 to 0.25 part of vitamin mixture, 7 to 9 parts of starch, 0.01 to 0.8 part of choline and 0.8 to 1.2 parts of resazurin. The method has the characteristics that the large-scale and high-flux active substance screening requirement can be met, and the TMA generation and related substrate metabolism quantitative control can be realized.

The invention provides a method for detecting related substances in an esdoxaban tosylate medicine, which is characterized in that the detection method is a high performance liquid chromatography, a dipotassium phosphate solution-acetonitrile is adopted as a mobile phase A, acetonitrile is adopted as a mobile phase B, and a gradient elution scheme is optimized. According to the present invention, by using the elaborately designed chromatographic conditions, particularly selecting the efficient octadecylsilane bonded silica gel filler as the stationary phase, and using the buffer solution and the organic solvent gradient elution system under the suitable pH value condition, the esdoxaban tosylate and the various potential related impurities thereof can be effectively separated. A dynamic gradient elution mode is adopted, good separation of a target compound and other impurities can be achieved within a short time, the analysis time is shortened, and the working efficiency of a laboratory is improved. The method can fully cover the types of impurities, and has good stability and reproducibility.

Improved cosmetic composition for the treatment of human perspiration and body odor. The present invention relates to a composition comprising, in a cosmetically acceptable aqueous medium, magnesium chloride, dipotassium phosphate, at least one anionic thickener selected from natural anionic gums, carrageenans, pectins, and phosphated starches, at least one modulating agent selected from mono- or polycarboxylic acids, optionally hydroxylated, in free or salted form, and at least one deodorizing active ingredient selected from a zinc salt, a bis(dihydropyridinyl)decan derivative, and mixtures thereof. It also relates to the associated process. Figure for abstract: none

The invention belongs to the technical field of functional food, and particularly relates to a composition with the effects of reducing blood sugar and blood fat and relaxing bowel and a preparation method and application of the composition. The composition comprises the following components in parts by weight, comprising the following raw materials: 10-20 parts of oat beta-glucan, 4-8 parts of a mulberry leaf extract, 2-6 parts of a white kidney bean extract, 3-7 parts of a cordyceps militaris extract, 1-5 parts of oat peptide, 4-8 parts of diglyceride oil, 1-3 parts of L-carnitine, 12-18 parts of konjaku flour, 8-12 parts of fructo-oligosaccharide, 6-10 parts of isomaltooligosacharide, 8-12 parts of yam flour, 2-4 parts of sodium caseinate and 1-3 parts of mono / diglycerol fatty acid ester. The feed additive is prepared from the following raw materials in parts by weight: 0.3 to 0.6 part of sodium stearoyl lactylate, 0.2 to 0.4 part of dipotassium phosphate, 0.1 to 0.3 part of silicon dioxide, 15 to 20 parts of psicose and 0.05 to 0.15 part of mogroside. The composition prepared by the invention has good effects in the aspects of reducing blood sugar, reducing blood fat, regulating intestinal flora, relaxing the bowels and the like.

The invention provides a flight control fertilizer composition for promoting wheat growth and increasing yield as well as a preparation method and application thereof, and relates to the technical field of agricultural fertilizers. The invention relates to a flight control fertilizer composition. The seaweed preservative is prepared from the following raw materials: 240 to 320 parts of an amino acid mixture, 40 to 65 parts of an enzymolysis seaweed extract, 6 to 15 parts of sodium dodecyl benzene sulfonate, 6 to 15 parts of sodium alkyl naphthalene sulfonate, 6 to 15 parts of sodium dodecyl sulfate, 110 to 160 parts of anhydrous dipotassium phosphate, 160 to 200 parts of potassium pyrophosphate, 20 to 40 parts of ethylene glycol and 6 to 15 parts of zinc sodium ethylene diamine tetraacetate. The amino acid mixture comprises the following components: aspartic acid, glutamic acid and arginine; the preparation method comprises the following steps: carrying out freeze thawing pretreatment on brown algae, and carrying out enzymolysis treatment by using cellulase, alginate lyase and mannase, so as to obtain the enzymolysis seaweed extract. All the components interact with one another, so that the synergistic effects of promoting wheat growth and germination and increasing the yield are achieved.

The invention belongs to the technical field of microbiological method water treatment denitrification materials, and particularly relates to a floating type sulfur carrier, a preparation method thereof and application of the floating type sulfur carrier in sulfur autotrophic denitrification nitrogen removal. The floating type sulfur carrier comprises a base body, the base body is of a non-magnetic hollow structure, the non-magnetic hollow structure is provided with a shell layer structure and a closed cavity formed by the shell layer structure, and the density of the base body is smaller than that of water; the sulfur-based composite donor layer is arranged on the outer surface of the shell structure, the sulfur-based composite donor layer comprises elemental sulfur, an auxiliary electron donor component, dipotassium phosphate and a buffer component, and the buffer component comprises carbonate and / or basic salt. By optimizing the structure of the floating type sulfur carrier and the composition of the sulfur-based composite donor layer, the denitrification efficiency and stability under the high-load working condition can be further improved.

The invention relates to the technical field of biological selenium enrichment, in particular to a production method and application of high-selenium ganoderma lucidum sporocarp. The high-selenium ganoderma lucidum sporocarp is prepared from a cultivation material prepared from sawdust, wheat bran, soybean meal powder, a selenium source, red granulated sugar and water. Wherein the selenium source is prepared from the following raw materials: dipotassium phosphate, magnesium sulfate, selenium salt, sorbitol, cyclodextrin and hydroxypropyl methyl cellulose. The selenium content in the ganoderma lucidum sporocarp obtained by the production method is not less than 185 mg / Kg, and the mass content of the ganoderma lucidum crude polysaccharide is not less than 6.3%.

The invention discloses a preparation method of an MICP-MISP synergistic mining spray dust suppressant, which comprises the following steps: step S1, inoculating and culturing a bacillus pasteurii bacterial solution, preserving at constant temperature, then adding anhydrous dipotassium phosphate, and uniformly stirring to obtain a bacterial solution; s2, adding urea, anhydrous calcium chloride, magnesium chloride and a surfactant into pure water, and uniformly mixing to obtain a cementing liquid; the invention further discloses the MICP and MISP synergistic spraying dust suppressant for the mine. The MICP and MISP synergistic spraying dust suppressant for the mine is prepared by adopting the preparation method of the MICP and MISP synergistic spraying dust suppressant for the mine. The invention further discloses application of the MICP and MISP synergistic mining spray dust suppressant as a spray dust suppressant in a coal mine. The dust suppression agent has the advantages of excellent dust suppression and dust suppression effects, environmental friendliness, low cost, high dust suppression agent utilization rate and the like.

The invention provides an efficient slow-release hydrogen microbial fermentation additive as well as a preparation method and application thereof, and belongs to the technical field of microbial fermention.The efficient slow-release hydrogen microbial fermentation additive comprises the following components in parts by weight: 1-10 parts of capsules; 0.001 to 6 parts of a slow-release hydrogen fermentation composition contained in the capsule; the slow-release hydrogen fermentation composition comprises a fermentable carbohydrate, the fermentable carbohydrate is configured to adjust the spatial distribution gradient of the active hydrogen concentration in the material, and the fermentable carbohydrate is 1-30 parts; the slow-release hydrogen fermentation composition further comprises a slow-release hydrogen material, and the slow-release hydrogen material comprises one or more of diammonium hydrogen phosphate, magnesium sulfate, magnesium chloride, magnesium hydroxide, magnesium hydride, diammonium hydrogen citrate, disodium hydrogen sulfate, sodium hydroxide, dipotassium phosphate and monopotassium phosphate. The additive is efficient, slow-released, stable and convenient to use, realizes uniform and directional release of hydrogen in bacterial liquid, and promotes growth and development of microorganisms.

The invention belongs to the field of microorganisms, and particularly relates to a screening culture medium and a screening method of beta-glucuronidase producing bacteria. The screening culture medium for producing the beta-glucuronidase bacteria is prepared from the following raw materials in parts by weight: 3 to 10 parts of ox bile crude extract, 12 to 24 parts of ammonium nitrate, 10 to 20 parts of sodium chloride, 1 to 10 parts of dipotassium phosphate, 1 to 8 parts of quercetin and 0.1 to 0.5 part of 5-bromo-4-chloro-3-indolyl-beta-D-gluconic acid glycoside. The crude ox bile extract is an ethanol extract of ox bile powder. According to the screening culture medium disclosed by the invention, the beta-glucuronidase producing bacteria can be rapidly separated from the ox bile, and the separated beta-glucuronidase producing bacteria are used for preparing the calculus bovis, so that the calculus bovis with high yield and quality can be obtained, and the production efficiency of the calculus bovis is improved.

The invention belongs to the technical field of sugar alcohol preparation, and relates to a preparation method of D-psicose, which comprises the following steps: sequentially carrying out activation, seed liquid culture and fermentation liquid culture treatment on glycerol tube strains of bacillus subtilis to obtain fermentation liquid; and directly pressing the obtained fermentation liquor with the volume ratio of 0.5-1.0% into an isomerization tank, and carrying out isomerization reaction on the fermentation liquor and a fructose solution to generate the required D-psicose. Wherein the fermentation culture medium comprises the following components: 30-60 g / L of glycerol, 30-50 g / L of soy peptone, 15-35 g / L of corn steep liquor dry powder, 0.5-3.0 g / L of ammonium citrate, 10-20 g / L of dipotassium phosphate, 1-5 g / L of monopotassium phosphate, 0.5-1.0 g / L of magnesium sulfate and 0.1-0.3 g / L of calcium chloride. According to the method disclosed by the invention, the fermentation liquor containing the D-psicose 3-epimerase is produced by utilizing the bacillus subtilis, and the fermentation liquor is directly pressed into the isomerization tank to carry out isomerization reaction to produce the D-psicose, so that not only is the fermentation time shortened, but also the process flow is simplified, and the production cost is reduced.

The invention discloses a preparation method of a microbial fertilizer, and belongs to the technical field of microbial fertilizers, the preparation method comprises the following steps: preparing a bacillus subtilis seed solution, preparing a paenibacillus polymyxa seed solution, treating corn steep liquor, preparing a fermentation medium, and fermenting; the step of treating the corn steep liquor comprises the following steps: mixing the corn steep liquor, distilled water and sodium carboxymethyl cellulose, stirring at room temperature, adjusting the pH value to 6.8-7.2, then adding calcium chloride, stirring, adding sodium carbonate, and stirring; the preparation of the fermentation culture medium comprises the following steps: mixing glucose, treated corn steep liquor, composite calcium carbonate, yeast extract powder, ammonium sulfate, disodium hydrogen phosphate, dipotassium phosphate, calcium chloride, magnesium sulfate, potassium chloride, ferrous sulfate, manganese sulfate and distilled water, adjusting the pH value to 6.8-7.2, and sterilizing; the number and the forming rate of spores in the prepared microbial fertilizer are high, and the prepared microbial fertilizer is good in quality stability, planting effect and appearance stability.

The invention relates to the technical field of culture media, in particular to a high-density culture medium for lactic acid bacteria as well as a preparation method and application of the high-density culture medium. Any one of soy peptone, yeast extract and tryptone; dipotassium phosphate or anhydrous sodium acetate; according to the present invention, the wort is adopted as the carbon source, the nitrogen source and the growth factor so as to promote the metabolism of the lactic acid bacteria, accelerate the propagation speed of the lactic acid bacteria and prolong the storage time of the lactic acid bacteria, such that the high-density culture of the lactic acid bacteria can be rapidly achieved; the cost of the culture medium is reduced, the innovative application of the fermentation technology in the fields of agriculture, livestock breeding and the like is widened, compared with an existing MRS broth culture medium, the culture medium prepared from wort-soy peptone, wort-yeast extract or wort-tryptone can be directly used for high-density culture, the cost is saved, the culture time is short, and the method is suitable for industrial production. And the viable count of the bacterial powder prepared by high-density fermentation is relatively high.

The application relates to a high-wax-content oil reservoir microbial activator and application thereof, and belongs to the technical field of oil exploitation; the components of the activator include glucose, sodium nitrate, sodium chloride, magnesium sulfate, yeast extract, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and trace elements; the activator can be injected into an oil production well to activate the microorganisms in the oil production well; the activated microorganisms can act on crude oil, thereby reducing the wax content and solidification point of high-wax-content crude oil, enhancing the flowability of the crude oil, improving the oil well yield, reducing lifting difficulty, and making the crude oil easy to flow out of a wellbore. The activator has the characteristics of low corrosion, no secondary pollution, low cost and the like, can be used for improving the single-well yield of high-wax-content oil reservoirs, and meets the needs of oilfield production.