160 results about "Corn steep liquor" patented technology

The invention discloses a method for producing bacillus velezensis through solid-state fermentation of distiller's grains, and belongs to the technical field of fermentation engineering. The preparation method comprises the following steps: carrying out acid-base modification, enzymolysis and stacking fermentation treatment on white spirit vinasse, adding bran and jerusalem artichoke powder, carrying out steam explosion, and adding corn steep liquor dry powder, urea and other substances to prepare a white spirit vinasse culture medium; and carrying out amplification culture on the bacillus velezensis subjected to activation and seed culture by using a white spirit vinasse culture medium, drying and collecting the bacillus velezensis. Experimental results show that the bacillus velezensis can be efficiently produced by utilizing the fermentation method provided by the invention, and the spore number of the bacillus velezensis is as high as 9.2 * 10 < 10 > cfu / g. The technical method for producing bacillus velezensis through fermentation effectively solves the problem of resource utilization of white spirit vinasse and the problems of high cost and sewage in the liquid fermentation process, and is low in fermentation cost and suitable for industrial production.

The invention discloses a culture method for increasing the protein content of aspergillus niger mycelia by using corn steep liquor as a culture medium, and belongs to the technical field of biological fermentation. Aiming at insufficient resource utilization of corn steep liquor and substitute protein production requirements, a corn processing by-product corn steep liquor is creatively adopted to directly replace a traditional PDB culture medium, and the mycelium protein content is remarkably increased by optimizing a fermentation system. Results show that the corn steep liquor is used as a culture medium, aspergillus niger with the preservation number of CCTCC M 2013703 is inoculated, and aspergillus niger mycelium protein can be obtained through fermentation. The protein content of the mycelium obtained by the process disclosed by the invention reaches 50.62-53.56%, and the total amino acid content is increased by 64-76%. According to the method, high-value conversion of agricultural corn byproducts is realized, and an efficient and low-cost industrial solution is provided for alternative protein production.

The invention provides a genetically engineered bacterium for producing bacterial cellulose as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of bioengineering. According to the invention, a K.medellensis expression vector is constructed, a glycerol transporter expression vector and a glycerol kinase expression vector are further constructed based on the K.medellensis expression vector, and the K.medellensis glycerol transporter expression vector and the glycerol kinase expression vector are transformed into K.medellensis to construct a genetically engineered bacterium for producing bacterial cellulose; glycerin and corn steep liquor are directly used as a carbon source and a nitrogen source respectively, and the fermentation conditions of the genetically engineered bacteria are further optimized, so that environment-friendly and low-cost synthesis of the bacterial cellulose is realized, the yield of the bacterial cellulose is remarkably increased, and the method has good practicability.

The present invention provides an essence containing chlorella extract and a preparation method thereof. The chlorella extract process comprises the following steps: raw material pulverization; enzymatic hydrolysis with cellulase and trypsin; primary fermentation using a mixture of Bacillus natto and Bacillus pumilus; addition of bamboo leaf extract, corn steep liquor, and sucrose, followed by inoculation with Rhodotorula glutinosus for secondary fermentation to obtain the chlorella extract. The mixed fermentation of multiple strains exhibits a synergistic effect, resulting in greater enrichment of natural products during the fermentation process compared to single-strain fermentation. Furthermore, the present invention provides an essence containing chlorella extract, glycerin, sodium hyaluronate, honey, and water, which exhibits significant moisturizing, antioxidant, and soothing effects.

An object of the present invention is to provide a lactase solution showing good permeation through a filtration filter, a lactase solution showing good permeation through a filtration filter and having a good residual lactase activity, and a lactase solution showing good permeation through a filtration filter and having a good residual lactase activity even when a lactase solution having a low protease activity and a low arylsulfatase activity as contaminant enzymes is used.A lactase solution containing any of (i) to (iii) below.(i) 0.0001 to 0.1 mass % of an unsaturated fatty acid or a salt thereof(ii) 0.0001 to 0.1 mass % of a saturated fatty acid salt(iii) 0.01 to 10 mass % of at least one selected from yeast extract, soybean peptone, pea protein, a casein degradation product, and corn steep liquor

The invention belongs to the technical field of food and medicine, and relates to application of corn germ concentrated powder in improvement of osteoarthritis. The invention particularly relates to application of corn germ concentrated powder, namely corn antioxidant multienzyme, in preparation of medicines or health-care foods for treating rheumatoid arthritis and osteoporosis. In the corn germ concentrated powder, superoxide dismutase / (U / g) is greater than or equal to 8500, glutathione peroxidase / (U / g) is greater than or equal to 5000, and catalase / (U / g) is greater than or equal to 750. The corn germ concentrated powder can also be prepared by the following method: cleaning corn, germinating, adding water into the germinated corn, grinding and pulping, adding a settling agent into the corn steep liquor, standing, taking supernate, adding part of sodium ascorbate, performing temperature-controlled concentration, adding the rest sodium ascorbate and a stabilizer into the concentrated solution, and uniformly mixing to obtain the corn germ concentrated powder. And drying at controlled temperature, crushing, and mixing with a proper amount of silicon dioxide. The prepared corn germ concentrated powder has the effect of resisting rheumatoid arthritis.

The invention discloses a preparation method of a compound microbial fertilizer, and belongs to the technical field of microbial fertilizers, the preparation method comprises the steps of starch gelatinization, strain activation, shake-flask culture, immobilization, fermentation and material mixing; the immobilization comprises the following steps: centrifuging a paenibacillus polymyxa shake-flask culture, and collecting wet thalli; mixing the wet thalli with normal saline, stirring at room temperature, adding the gelatinized starch solution, and continuously stirring to obtain a mixed bacterial solution; stirring a calcium chloride aqueous solution at 30-35 DEG C, dropwise adding the mixed bacteria solution, continuously stirring after dropwise adding, filtering, and taking filter residues to obtain immobilized paenibacillus polymyxa; the compound microbial fertilizer prepared by the invention is high in viable count, and the viable count is slightly influenced by the content of each substance in the corn steep liquor.

The invention relates to a method for deep processing of corn by using a pressurized soaking method, in particular to a method for preparing dextrin, syrup or sugar, in which the pressurized soaking method is used for replacing a soaking process of a traditional wet corn processing method, so that the corn quickly absorbs water and swells on the premise of not damaging the quality of germs and endosperm and not discharging waste water and waste gas, and the dextrin, the syrup or the sugar is prepared. The process requirements of a wet corn processing method are met, germs and corn husks are further removed, and the obtained germs and corn husks are refined, so that the germs and the corn husks respectively reach commercial selling standards; directly mixing and liquefying the corn slurry without germs and corn husks; the resulting liquefied liquid may be dried to produce dextrins, or further saccharified, refined, concentrated to produce syrup, or optionally further produce sugars.

This invention relates to the fields of agricultural product processing and bio-feed technology, specifically to a method and application for detoxifying corn steep liquor. The method includes: subjecting a mixture of corn steep liquor, a composite color-protecting agent, and a composite catalyst to a first reaction; allowing the reaction system after the first reaction to stand in a mixture with a composite adsorbent; subjecting the mixture containing the reaction system after the stand-in reaction and a composite enzyme preparation to stand-in treatment, mixing treatment, and enzymatic hydrolysis; and subjecting the mixture containing the reaction system after the enzymatic hydrolysis and an activated composite microbial agent to anaerobic fermentation. This method employs a multi-stage chemical-enzyme-fermentation linkage process, achieving efficient detoxification of corn steep liquor while significantly improving flavor and nutrition. This method synergistically removes mycotoxins and sulfites, and enhances palatability and probiotic value through fermentation transformation of off-flavor substances and enrichment of small peptides and lactic acid. The process is simple and requires no adsorbent separation.

The invention provides an auxin formula for promoting early germination of Chinese cabbage, which is characterized by comprising corn steep liquor, brown sugar, clear water and the like, the final germination rate of Chinese cabbage seeds in clear water and plant auxin is the same, but the third day is the first day of seed germination, and the fourth day is the second day of seed germination. The germination rate of the seeds in the auxin is higher than that in clear water, which indicates that the auxin can stimulate the seeds to break dormancy and germinate in advance.

The invention discloses a genetically engineered bacterium and method for producing succinic acid by fermenting corn straw hydrolysate, and the method comprises the following specific steps: 1, preparing the corn straw hydrolysate: treating 1-2% NaOH solution at 110-130 DEG C for 0.5-1.5 h, with the lignin removal rate being greater than or equal to 70% and the cellulose retention rate being greater than or equal to 85%; step 2, aerobic fermentation of the genetically engineered bacteria: a culture medium contains 8-12 g / L of glucose and 3-7 g / L of yeast extract or corn steep liquor, and the genetically engineered bacteria are cultured at 35-39 DEG C and 180-220 rpm until OD600 is 2.5-3.5; and 3, anaerobic fermentation of the bacterial strain: taking the corn straw hydrolysate obtained in the step 1 as a carbon source, and adding a buffering agent into the genetically engineered bacteria for anaerobic fermentation. According to the recombinant escherichia coli E.coli SUCC-01, succinic acid is produced by knocking out an acetic acid synthesis gene pta / ackA and overexpressing an sdhA / mdh gene and combining with corn straw hydrolysate through fermentation, the yield of the succinic acid can reach 80 g / L, the total sugar utilization rate is larger than or equal to 90%, the by-product acetic acid is smaller than or equal to 12 g / L, and the yield is increased by 60% compared with that of a wild type strain.

The application provides corn steep liquor for fermentation and a preparation method and application thereof, and belongs to the technical field of biology.The preparation of the corn steep liquor for fermentation comprises the following steps: hydrolyzing corn steep liquor, homogenizing the hydrolyzed corn steep liquor, and then heating and filtering the homogenized corn steep liquor to obtain the corn steep liquor for fermentation.The hydrolysis and high-pressure homogenization process are combined, microorganisms remaining in the corn steep liquor can be effectively cracked, macromolecular proteins and other substances can be decomposed into nutrients which are easy to utilize, the risk of contamination of a fermentation tank due to incomplete sterilization of the corn steep liquor is reduced, and the conversion rate of a fermentation product is improved.

The invention belongs to the technical field of feed biology, and particularly relates to an aflatoxin oxidase mutant as well as a coding gene and application thereof. According to the three-point combined mutant E188R-S210G-T418A provided by the invention, the enzyme activity is 177% of that of a female parent BlCotA; after water bath is carried out for 30 minutes at 80 DEG C, the enzyme activity is 68% of the initial enzyme activity, and the thermal stability is superior to that of a female parent BlCotA; after the mutant E188R-S210 G-T418A is used for treating raw milk for 1 hour, 2 hours and 4 hours, the residual quantity of the AFM1 is respectively reduced to 0.31 ng / mL, 0.12 ng / mL and 0.01 ng / mL; after corn steep liquor is treated by the mutant E188R-S210G-T418A, the degradation rates of AFB1, AFB2, AFG1 and AFG2 are respectively 93.71 percent, 93.95 percent, 96.79 percent and 97.58 percent.

The invention relates to the technical field of microbial oil recovery, in particular to bacillus subtilis 20P3 LZT, fermentation liquor and application of the bacillus subtilis 20P3 LZT to microbial oil recovery, the bacillus subtilis 20P3 LZT is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30366. The bacillus subtilis 20P3 LZT is found for the first time, can generate surfactant lipopeptide, can grow on the basis of molasses or corn steep liquor dry powder as a carbon source, has good emulsification and viscosity reduction performance on crude oil, has the emulsification and viscosity reduction rate of 70% on thick oil, can reduce the viscosity of the crude oil and enhance the fluidity of the crude oil, and can be used for preparing crude oil. On one hand, the oil-water fluidity ratio is reduced, and on the other hand, the emulsified crude oil has the effects of profile control and liquid flow diversion, so that the crude oil recovery rate is indirectly improved, and a new strain and a material are provided for a microbial oil recovery technology.

The invention relates to the technical field of fertilizers, in particular to a microbial amino acid-containing water-soluble fertilizer and a preparation method thereof. The method comprises the following steps: providing a first corn steep liquor and a second corn steep liquor; adding a Trichoderma harzianum-Purpureocillium lilacinum complex microbial inoculant and lactic acid bacteria into the first corn steep liquor, and carrying out first fermentation to obtain a first fermentation broth; mixing the first fermentation liquor with the second corn steep liquor to obtain mixed corn steep liquor; adding bacillus subtilis into the mixed corn steep liquor, and carrying out second fermentation to obtain second fermentation liquor; and sequentially adding a nitrogen fertilizer, a phosphate fertilizer and a potash fertilizer into the second fermentation liquor to obtain the water-soluble fertilizer containing microbial amino acids. The microbial amino acid-containing water-soluble fertilizer prepared by the preparation method provided by the invention can effectively improve the yield and quality of crops, improve the soil structure, enhance the soil fertility, promote the root development of the crops and improve the resistance of the crops to adversity.

The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor, and belongs to the technical field of corn deep processing and detoxification. The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor. The method comprises the following steps: adjusting the pH value of thick corn steep liquor by using an alkaline solution; adding a solid catalyst into the corn steep liquor; carrying out detoxification treatment by combining heterogeneous ozone oxidation reaction with microbubbles; and separating and recovering the solid catalyst to obtain the detoxified corn steep liquor. The vomitoxin content of the treated concentrated corn pulp is smaller than 1000 g / kg, the degradation rate is larger than 93%, the zearalenone toxin content is smaller than 33 g / kg, and most of the zearalenone toxin is removed. According to the method disclosed by the invention, the content of fungaltoxin in the corn steep liquor is reduced, so that the economic value of corn byproducts is improved, the economic benefit of grain trade is favorably improved, and the storage period and value of produced products are improved.

The present invention belongs to the technical field of zearalenone extraction and relates to a method for extracting zearalenone from corn steep liquor to prepare a crude standard. The method comprises centrifuging corn steep liquor containing ZEN to obtain a supernatant; treating the supernatant using a PA66 membrane to obtain a PA66 membrane enriched with ZEN; eluting the PA66 membrane enriched with ZEN to obtain an eluate; and purifying the eluate by rotary evaporation to obtain a crude ZEN standard. The PA66 membrane is used to enrich and extract ZEN from ZEN-contaminated corn byproducts, achieving an enrichment and extraction efficiency exceeding 90%, providing a reliable and effective method for the high-value utilization of corn byproducts. The PA66 membrane is used to efficiently and selectively adsorb and enrich ZEN to initially prepare a crude ZEN standard, providing a new approach to the preparation of ZEN standard products.

The invention relates to a preparation method of a corn steep liquor fertilizer, in particular to a preparation method of a corn steep liquor fertilizer based on circulating membrane separation enhanced enzymolysis. In order to solve the problem that the existing method for preparing the fertilizer from the corn steep liquor is extensive, the method comprises the following steps: 1, diluting the corn steep liquor, adding a biological flocculant, adding enzyme, and separating by a circulating membrane to respectively obtain filtrate 1 and concentrated liquor 1; adding enzyme into the filtrate 1, and separating by a circulating membrane to respectively obtain filtrate 2 and concentrated liquid 2; adding charcoal and a compound material into the concentrated liquid 1, uniformly stirring, drying, extruding and granulating to obtain a solid fertilizer; and adding the concentrated liquid 2 into the compound material to obtain the liquid fertilizer. No waste liquid or waste is generated in the whole process, the large-molecular-weight low-solubility slow-degradation solid fertilizer and the small-molecular-weight high-solubility easily-absorbable liquid fertilizer are prepared according to different absorption characteristics, and the method is applied to the field of fertilizer preparation.

The application provides application of neutral protease in degradation of fumonisin, and belongs to the technical field of biology, and it is verified through experiments that the neutral protease has good degradation effect on FB1. The application also provides a compound enzyme preparation which comprises the following components: 1-5% neutral protease, 5-20% montmorillonite, 75-94% soluble starch, and only by adding 0.5wt%-1.5wt% of the compound enzyme preparation, the efficient degradation of FB1 in common contaminated feed can be realized; specifically, the degradation rate of the compound enzyme preparation on FB1 in corn flour can reach 95.73%, the degradation rate of the compound enzyme preparation on FB1 in DDGS can reach 96.43%, the degradation rate of the compound enzyme preparation on FB1 in corn steep liquor can reach 64.74%, and the degradation rate of the compound enzyme preparation on FB1 in corn cob powder can reach 93.74%.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a digestible biological protein fermentation material. According to the fermentation method, candida utilis serves as a fermentation strain, industrial and agricultural waste glucose mother liquor and corn steep liquor serve as substrates, high-yield mycoprotein is obtained under the relative low-temperature fermentation condition of 20-25 DEG C, and the obtained fermentation liquor is subjected to single enzymolysis, so that a fermentation material easier to digest is obtained. When weaned piglets are fed with the obtained fermented material, the diarrhea rate of the piglets can be effectively reduced.

The invention relates to the technical field of biological fermentation. The invention provides a strain of Geotrichum candidum Do-Ya-2-12, and the preservation number of the strain of Geotrichum candidum Do-Ya-2-12 is CGMCC (China General Microbiological Culture Collection Center) No. 33104. The strain of the geotrichum candidum Do-Ya-2-12 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the strain is CGMCC No.33104, the preservation time of the strain is December 17, 2024, the preservation center is called CGMCC for short, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain provided by the invention can replace a conventionally used carbon source and nitrogen source with a corn soaking solution or corn steep liquor, is environment-friendly, improves the conversion rate of lactic acid by microorganisms such as yeast, reduces the production cost, simplifies the production process, and realizes efficient output of single-cell protein.

The invention discloses recombinant klebsiella pneumoniae with high yield of 3-hydracrylic acid as well as a construction method and application of the recombinant klebsiella pneumoniae, and belongs to the technical field of genetic engineering. The construction method comprises the following steps: by taking klebsiella pneumoniae as an original strain, introducing kgsadh genes for coding alpha-ketoglutarate semialdehyde dehydrogenase, and knocking out 1, 2, 3, 4-tetramethyl-3-oxoglutarate; the method comprises the following steps: constructing recombinant klebsiella pneumoniae for high yield of 3-hydracrylic acid by using one or more of a dhaT gene of 1, 3-propylene glycol dehydrogenase, an ldh gene for coding L-lactic dehydrogenase, an ldhA gene for coding 2-hydracrylic acid dehydrogenase and a yqhD gene for coding alcohol dehydrogenase. Compared with an initial strain and a recombinant strain, the 3-HP of the recombinant strain is remarkably improved, and the yield of the 3-HP is the highest when an organic nitrogen source is determined to be corn steep liquor or the concentration of glycerol is controlled to be 15-25g / L through organic nitrogen source optimization and glycerol concentration control, so that a new strain choice is provided for high yield of the 3-hydracrylic acid.

The invention relates to a corn steep liquor dry powder production process and equipment. The production process comprises the following steps: 1, a corn steep liquor production process: (1) raw material pretreatment, (2) soaking process, and (3) soaking liquid separation; 2, concentrating the corn steep liquor; and 3, drying the concentrated corn steep liquor. The production equipment comprises a low-temperature vacuum concentrator, an evaporation concentration system with a heat pump system and a vacuum liquid belt dryer. The corn steep liquor concentration efficiency is high, the concentration effect is good, energy consumption is low, waste gas pollution is avoided, active ingredients of the corn steep liquor are well kept, and the product quality is excellent.

The application provides application of neutral protease in degradation of fumonisin, and belongs to the technical field of biology, and it is verified through experiments that the neutral protease has good degradation effect on FB1. The application also provides a compound enzyme preparation which comprises the following components: 1-5% neutral protease, 5-20% montmorillonite, 75-94% soluble starch, and only by adding 0.5wt%-1.5wt% of the compound enzyme preparation, the efficient degradation of FB1 in common contaminated feed can be realized; specifically, the degradation rate of the compound enzyme preparation on FB1 in corn flour can reach 95.73%, the degradation rate of the compound enzyme preparation on FB1 in DDGS can reach 96.43%, the degradation rate of the compound enzyme preparation on FB1 in corn steep liquor can reach 64.74%, and the degradation rate of the compound enzyme preparation on FB1 in corn cob powder can reach 93.74%.

The present invention discloses a feed medium and a feeding method for producing D-psicose-3 epimerase, and belongs to the field of microbial fermentation technology. The present invention adopts a specific proportion of glucose, corn steep liquor, soybean cake powder and trace elements to make a feed medium with a certain carbon-nitrogen ratio and a quick-acting nitrogen source, a slow-acting nitrogen source and trace elements, and uses Bacillus subtilis as a fermentation strain. During the fermentation process, feed is added in a fed-batch manner, and pH-dissolved oxygen-feed coupling regulation is simultaneously performed during the fermentation process to increase the cell concentration and the activity of D-psicose-3 epimerase as much as possible. The method of the present invention is simple to operate, reduces production costs, shortens the fermentation cycle, and significantly improves the enzyme activity of the target enzyme.

The present invention relates to a method for treating a corn steep liquor filter cake to produce high added value organic matter that is useful as a biostimulant in plants. The method comprises adding a preservative agent to a corn steep liquor filter cake and homogenising the whole to properly mix it. A drying and milling process is performed to produce a solid corn steep liquor cake with biostimulant activity.

This invention relates to microbial culture. It provides a *Bacillus parabregas* culture medium and its applications, wherein the medium comprises at least one of glycerol, yeast extract, soybean peptone, corn steep liquor powder, ammonium sulfate, magnesium sulfate, and calcium carbonate. The *Bacillus parabregas* culture medium of this invention utilizes glycerol as a carbon source and yeast extract, soybean peptone, etc., as nitrogen sources for large-scale liquid fermentation of *Bacillus parabregas*, achieving a total bacitracin content of over 10.5 g / L. The proportions of each component can be controlled by feeding. Using this medium for *Bacillus parabregas* fermentation results in high bacitracin yield and controllable composition. The medium formulation is simple, inexpensive, readily available, safe, and environmentally friendly. The fermentation process is simple and controllable, with a short fermentation cycle, facilitating industrial-scale production and possessing high application value.

The invention relates to the technical field of wastewater treatment, and provides a method for producing water-soluble solid protein from corn soaking water. The method comprises the following steps: performing ultrafiltration on corn soaking water to obtain ultrafiltration permeate liquid and ultrafiltration concentrated liquid; concentrating the ultrafiltration permeate to obtain a first concentrated solution; adjusting the pH value of the ultrafiltration concentrated liquor, and filtering to obtain first filter residues and first filtrate; and mixing the first filtrate and the first concentrated solution, and performing enzymolysis, crystallization, filtration, nanofiltration, concentration and spray granulation to obtain the water-soluble solid protein. According to the method provided by the invention, effective treatment of a large amount of corn soaking water can be realized, meanwhile, high-quality water-soluble solid protein is produced, and high value-added utilization of the corn soaking water is realized; furthermore, the main component of the obtained first filter residue is calcium magnesium phytate, the main component of the obtained second filter residue is magnesium lactate, the first filter residue and the second filter residue can be sold as solid organic fertilizer, and the nanofiltration permeate liquid can be sold as liquid organic fertilizer, so that the economic benefit is further improved.

The invention discloses a fermentation method for performing enzymolysis on a corn steep liquor yeast culture medium by using a glucose mother liquor. The fermentation method comprises the following steps: 1) treating the glucose mother liquor; (2) preparing enzymolysis corn steep liquor; (3) preparing a nutritive salt dissolving solution; (4) preparing vitamin combined fed-batch liquid; and (5) fermenting. Corn protein in the corn steep liquor is subjected to enzymolysis through corn protease, protein which cannot be utilized by saccharomycetes is converted into available amino acid and small molecule peptide, effective components in the corn steep liquor are fully utilized, the technical problem that the utilization rate of the effective components in the corn steep liquor is low is solved, meanwhile, the adding amount of nitrogen and phosphorus sources is reduced, and the production cost is reduced. And the production cost is directly reduced. The raw materials are easy to obtain, the process is reliable and stable, the corn steep liquor yeast culture medium is subjected to enzymolysis by adopting the glucose mother liquor, compared with a molasses yeast culture medium, COD of wastewater generated in the production process is greatly reduced, and the sewage treatment cost is reduced.

The utility model relates to the technical field of biological fermentation equipment, in particular to a corn steep liquor heating tank which comprises a tank body, a stirring device is arranged in the tank body, a heating assembly is arranged at the bottom of the stirring device, the stirring device comprises a stirring shaft, and a stirring mechanism is arranged at the bottom of the stirring shaft. The stirring shaft comprises an outer shaft and an inner shaft which are fixedly sleeved together, and the inner shaft extends out of the outer shaft; the stirring mechanism comprises a stirring rod and an anchor type stirring paddle, the stirring rod is installed on the outer shaft through a fixing sleeve and located in the middle of the tank body, and the anchor type stirring paddle is located at the bottom of the stirring rod. According to the utility model, when the stirring shaft rotates, the stirring rod is matched with the anchor type stirring paddle, so that corn steep liquor can be better stirred uniformly; the heating area of corn steep liquor in the tank body is increased, the heating time is shortened, and resources are saved.