16 results about "Corn steep liquor" patented technology

This invention relates to the fields of agricultural product processing and bio-feed technology, specifically to a method and application for detoxifying corn steep liquor. The method includes: subjecting a mixture of corn steep liquor, a composite color-protecting agent, and a composite catalyst to a first reaction; allowing the reaction system after the first reaction to stand in a mixture with a composite adsorbent; subjecting the mixture containing the reaction system after the stand-in reaction and a composite enzyme preparation to stand-in treatment, mixing treatment, and enzymatic hydrolysis; and subjecting the mixture containing the reaction system after the enzymatic hydrolysis and an activated composite microbial agent to anaerobic fermentation. This method employs a multi-stage chemical-enzyme-fermentation linkage process, achieving efficient detoxification of corn steep liquor while significantly improving flavor and nutrition. This method synergistically removes mycotoxins and sulfites, and enhances palatability and probiotic value through fermentation transformation of off-flavor substances and enrichment of small peptides and lactic acid. The process is simple and requires no adsorbent separation.

The application provides application of neutral protease in degradation of fumonisin, and belongs to the technical field of biology, and it is verified through experiments that the neutral protease has good degradation effect on FB1. The application also provides a compound enzyme preparation which comprises the following components: 1-5% neutral protease, 5-20% montmorillonite, 75-94% soluble starch, and only by adding 0.5wt%-1.5wt% of the compound enzyme preparation, the efficient degradation of FB1 in common contaminated feed can be realized; specifically, the degradation rate of the compound enzyme preparation on FB1 in corn flour can reach 95.73%, the degradation rate of the compound enzyme preparation on FB1 in DDGS can reach 96.43%, the degradation rate of the compound enzyme preparation on FB1 in corn steep liquor can reach 64.74%, and the degradation rate of the compound enzyme preparation on FB1 in corn cob powder can reach 93.74%.

This invention relates to microbial culture. It provides a *Bacillus parabregas* culture medium and its applications, wherein the medium comprises at least one of glycerol, yeast extract, soybean peptone, corn steep liquor powder, ammonium sulfate, magnesium sulfate, and calcium carbonate. The *Bacillus parabregas* culture medium of this invention utilizes glycerol as a carbon source and yeast extract, soybean peptone, etc., as nitrogen sources for large-scale liquid fermentation of *Bacillus parabregas*, achieving a total bacitracin content of over 10.5 g / L. The proportions of each component can be controlled by feeding. Using this medium for *Bacillus parabregas* fermentation results in high bacitracin yield and controllable composition. The medium formulation is simple, inexpensive, readily available, safe, and environmentally friendly. The fermentation process is simple and controllable, with a short fermentation cycle, facilitating industrial-scale production and possessing high application value.

The utility model discloses a corn pulp fermentation process in the on -line metering device of bubble enemy, including the measuring jar, and the lower part of measuring jar is installed with water inlet pipe and bubble enemy granule feed pipe respectively, and the inner wall of measuring jar is installed with support, and the support is installed with the buffer board corresponding with the water outlet end of water inlet pipe, and the filter screen is installed in the measuring jar, and the aperture of filter screen is less than the maximum diameter of qualified bubble enemy granule, and the rotating rod is installed in the measuring jar, and the liftable floating plate is installed on the rotating rod, and the density of floating plate is less than the density of bubble enemy solution, and floating plate is located above the filter screen. The utility model has the beneficial effects that: through setting up support and buffer board cooperation can effectively reduce the water flow impact force of water outlet end of water inlet pipe, make the water flow of water inlet pipe steady enter the measuring jar, and the density of setting up floating plate is less than the density of bubble enemy solution can be in the process of liquid inlet and float steadily, thereby avoiding the deviation of liquid level sensor measurement caused by upper liquid level swing.

The invention relates to a method for obtaining a gramicidin S extract from spore-forming Bacillus belonging to the genus Aneurinibacillus, characterised by comprising a solid-liquid extraction step using a solvent selected from the group comprising lactic acid, acetic acid, a biosurfactant extract obtained from corn steep liquors, or combinations thereof, at a temperature equal to or less than 40 °C. The invention also relates to the use of the gramicidin S extract obtained as an antimicrobial agent in the cosmetic and agrochemical industries, and as an antibiotic in the pharmaceutical industry.

This invention relates to the field of kasugamycin production technology, specifically disclosing a kasugamycin fermentation culture medium and a kasugamycin fermentation method. The fermentation basal culture medium and the supplemental culture medium are composed of the following components by volume percentage: 5-8% soybean meal, 0.1-0.5% sodium glutamate, 0.26-0.5% potassium dihydrogen phosphate, 0.25-0.5% corn steep liquor, 7-9% soybean oil, and 1-3% maltodextrin. The supplemental culture medium consists of 0.5-1.6% soybean meal and 0.1-0.3% inositol. This invention, by optimizing the basal culture medium formula and combining it with the addition of soybean meal and inositol in the middle and late stages, effectively increases the fermentation potency of kasugamycin. Simultaneously, it avoids the problem of increased fermentation broth viscosity and impaired dissolved oxygen transfer caused by an unreasonable carbon-nitrogen ratio in traditional fermentation processes. It significantly reduces the viscosity of the fermentation broth, improves oxygen supply, and is beneficial for cell growth and the synthesis of secondary metabolites.

This invention discloses an in-situ enzymatic hydrolysis and saccharification method for corn straw, belonging to the field of biochemical technology. The method consists of the following steps: pretreatment, activation and expansion culture, preparation of auxiliary agents, preparation of crude enzyme solution, and enzymatic hydrolysis and saccharification. The enzymatic hydrolysis and saccharification involves mixing pretreated corn straw powder, corn steep liquor powder, potassium hydroxide, ammonium sulfate, magnesium sulfate, a first portion of water, and the crude enzyme solution, stirring at 27-29℃ and 180-220 rpm for 30-40 minutes, adjusting the pH to 4.7-4.9, sterilizing, and obtaining a fermentation medium. The auxiliary agents, seed liquid, and a second portion of water are mixed, stirred at 27-29℃ and 180-220 rpm, added to the fermentation medium, and cultured with shaking at 27-29℃ and 140-160 rpm for 6-7 days. This invention can achieve high-solids enzymatic hydrolysis with a high specific sugar yield, and the specific sugar yield results show little difference when treating different batches of corn straw.

The present application is suitable for the technical field of grain processing quality control, and provides a method and system for predicting the content of vomitoxin in corn syrup, which comprises the following steps: mixing contaminated corn raw materials with a soaking liquid containing SO2 source and lactic acid, and simulating the countercurrent soaking process of corn wet processing, during which, every time interval, the operation of pouring the syrup and the collection of soaking liquid samples are carried out; determining the content of vomitoxin in the soaking liquid samples to obtain determination data; according to the determination data, a migration model of vomitoxin from corn raw materials to corn syrup in the countercurrent soaking process of corn wet processing is constructed, and the model parameters are obtained by fitting; according to the model parameters, a prediction model of the content of vomitoxin in corn syrup is determined, and the content of vomitoxin in corn syrup in the corn wet processing is predicted according to the prediction model. The method can guide the optimization of corn syrup production process, effectively control vomitoxin in the processing process, and ultimately ensure product quality and safety.

This invention discloses a fermentation medium and its application in the fermentation process for increasing 7-DHC production in *Saccharomyces cerevisiae*. By optimizing key nutrients and precisely controlling fermentation conditions, the production efficiency of 7-DHC is significantly improved while reducing costs. The medium is carefully formulated with proportions of glucose, corn steep liquor, amino acids (such as L-leucine and L-histidine), vitamins, and metal ions to ensure an optimal growth environment for *Saccharomyces cerevisiae*. During fermentation, dissolved oxygen levels are maintained by adjusting parameters such as temperature, pH, fermentation speed, and aeration rate. A high-concentration glucose solution is added at appropriate residual sugar concentrations, and the ethanol concentration is strictly controlled to avoid its inhibitory effect on cells. Furthermore, using molasses as an alternative carbon source is not only economical but also ensures efficient 7-DHC synthesis. This enables a transition from small-scale trials to large-scale production, promoting its widespread application in the pharmaceutical and health product fields.

This invention discloses an anti-caking device for conveying fermented corn syrup powder, specifically relating to the field of fermented corn syrup powder conveying technology. It includes a conveying cylinder with an inlet pipe at one top end and an outlet pipe at one bottom end. This invention utilizes multiple sets of alternating flaps arranged between spiral rings. These flaps rotate synchronously with the spiral blades, turbulenting and dispersing the fermented corn syrup powder within the semi-enclosed chamber formed by the spiral rings. Simultaneously, each set of second connecting shafts drives the flaps to reciprocate and oscillate within each chamber. Through this rotational turbulence and reciprocating oscillation, the advancing fermented corn syrup powder is continuously disturbed and dispersed, effectively preventing the powder from forming dense clumps under pressure, eliminating localized static accumulation, and improving the problem of insufficient agitation leading to the continuous growth of small clumps. Ultimately, this effectively achieves an anti-caking effect during the conveying process of fermented corn syrup powder.

The application provides corn syrup fermentation and application thereof in preparation of water-soluble nitrogen fertilizer, and belongs to the technical field of water-soluble fertilizer.The preparation method of the Rhizopus kind koji provided by the application comprises the following steps: mixing Rhizopus spore suspension and culture medium, and carrying out fermentation culture at 28 DEG C to 40 DEG C for 48 h to 108 h; the culture medium is composed of dry materials and liquid materials, the dry materials are composed of soybean meal and bran, and the liquid materials are composed of glucose mother liquor, corn syrup and water.The preparation method of the Rhizopus kind koji can improve the neutral protease activity in the Rhizopus kind koji, so as to improve the subsequent fermentation efficiency of corn syrup and improve the functional activity of the fermentation product obtained by corn syrup fermentation.The corn syrup fermentation provided by the application can improve the small peptide content in the corn syrup fermentation product.The corn syrup fermentation provided by the application can replace nitrogen fertilizer to promote the growth and development of soybean.

This invention discloses a method for fermenting and culturing *Yarrowia lipolytica* for feed using waste liquor from corn processing for sugar refining. The invention uses glucose crystallization secondary mother liquor and corn steep liquor, the main waste products from corn processing for sugar refining, as the carbon and nitrogen sources, respectively. *Yarrowia lipolytica* is cultured in a fermenter using an aerobic fed-batch method. The required culture medium and feed solution, apart from necessary pH adjustment and the addition of an antifoaming agent, only require the secondary mother liquor and corn steep liquor. With the culture medium and fermentation operations remaining the same in the early stages of fermentation, the only change in the later feeding stage is the ratio of the secondary mother liquor and corn steep liquor in the feed solution. This achieves the goals of high lipid accumulation, high protein accumulation, and increased cell yield in the cultured *Yarrowia lipolytica* cells, without altering the characteristics of the accumulated lipids being high in polyunsaturated fatty acids and the proteins being rich in essential amino acids such as lysine, serine, and leucine.

The utility model discloses a corn steep liquor supplementing tank which comprises a tank body, the tank body comprises a tank top and a tank body, a machine base is arranged in the center of the tank top, a stirring driving device is installed on the machine base, the stirring driving device is connected with a stirring shaft extending into the tank body through a coupler, and a first stirrer and a second stirrer are arranged in the middle and at the lower end of the stirring shaft respectively. A feeding hole is formed in one side of the tank top, a hoisting device is annularly arranged on the periphery of the tank top, a flange plate and a sealing gasket are arranged on the butt joint surface of the tank body and the tank top, a jacket is arranged outside the tank body, a steam inlet is formed in the top side of the jacket, a condensed water outlet is formed in the bottom of the jacket, and a discharging hole is formed in the bottom of the tank body. The corn steep liquor supplementing tank can effectively reduce scaling and equipment cleaning frequency, has the advantages of being convenient to clean, good in cleaning effect and the like, and can effectively guarantee normal operation of equipment and maintain production efficiency and product quality.

The invention provides a corn steep liquor protein solution and a preparation method thereof. Firstly, a treatment method of the corn steep liquor residual liquid subjected to protein separation treatment is developed, and the treatment method comprises the step of treating the corn steep liquor residual liquid by adopting a composite column with a molecular sieve layer as an upper layer and a cation exchange resin layer as a lower layer. According to the scheme, the ash content can be remarkably reduced while protein is not lost. The invention further provides a method for preparing the corn steep liquor protein solution, which comprises the steps of removing phytic acid from the corn steep liquor, treating residual liquid of the corn steep liquor and recovering protein, and in a product, on a dry basis, the phytic acid is less than or equal to 1 percent, the ash content is less than or equal to 1 percent, the protein content is more than 65 percent, the phytic acid clearance rate is more than 90 percent, the ash content clearance rate is more than 95 percent, and the protein extraction rate is more than 80 percent. The protein content is increased while the phytic acid content and the ash content in the corn steep liquor are remarkably reduced, the problem that the corn steep liquor affects mineral absorption and palatability of animals can be solved, and high-value utilization of the corn steep liquor is achieved.

Disclosed are agrochemical compositions for ready-to-use (LFR) liquid fertilizers containing corn steep liquor. These compositions contain agrochemical active ingredients, dispersants, liquid fertilizer compatibility agents containing corn steep liquor, freeze-thaw stabilizers, and optionally preservatives.