190 results about "Corn steep liquor" patented technology

The invention discloses a method for producing bacillus velezensis through solid-state fermentation of distiller's grains, and belongs to the technical field of fermentation engineering. The preparation method comprises the following steps: carrying out acid-base modification, enzymolysis and stacking fermentation treatment on white spirit vinasse, adding bran and jerusalem artichoke powder, carrying out steam explosion, and adding corn steep liquor dry powder, urea and other substances to prepare a white spirit vinasse culture medium; and carrying out amplification culture on the bacillus velezensis subjected to activation and seed culture by using a white spirit vinasse culture medium, drying and collecting the bacillus velezensis. Experimental results show that the bacillus velezensis can be efficiently produced by utilizing the fermentation method provided by the invention, and the spore number of the bacillus velezensis is as high as 9.2 * 10 < 10 > cfu / g. The technical method for producing bacillus velezensis through fermentation effectively solves the problem of resource utilization of white spirit vinasse and the problems of high cost and sewage in the liquid fermentation process, and is low in fermentation cost and suitable for industrial production.

The invention discloses a culture method for increasing the protein content of aspergillus niger mycelia by using corn steep liquor as a culture medium, and belongs to the technical field of biological fermentation. Aiming at insufficient resource utilization of corn steep liquor and substitute protein production requirements, a corn processing by-product corn steep liquor is creatively adopted to directly replace a traditional PDB culture medium, and the mycelium protein content is remarkably increased by optimizing a fermentation system. Results show that the corn steep liquor is used as a culture medium, aspergillus niger with the preservation number of CCTCC M 2013703 is inoculated, and aspergillus niger mycelium protein can be obtained through fermentation. The protein content of the mycelium obtained by the process disclosed by the invention reaches 50.62-53.56%, and the total amino acid content is increased by 64-76%. According to the method, high-value conversion of agricultural corn byproducts is realized, and an efficient and low-cost industrial solution is provided for alternative protein production.

The invention discloses a culture medium for improving the fermentation titer of lincomycin and a culture method, and relates to the field of microbial fermentation. Lincomycin streptomycete is taken as a starting strain, biological nitrogen is added into seeds and a fermentation culture medium basic material, and biological nitrogen and a trace element TE are added into a supplementary material, so that an obvious regulating effect on promoting carbon and nitrogen source metabolism in a fermentation process is achieved, and proper conditions are provided for thallus growth and element production; by adjusting the proportion of biological nitrogen, soybean meal, ammonium sulfate and corn steep liquor supplemented into the mixed nitrogen material in the process, the maintainability of thalli is further improved, and fermentation production of lincomycin is facilitated. The method is suitable for stably and efficiently producing lincomycin on a large scale, and has the advantages of simplicity in operation, remarkable optimization effect and easiness in popularization.

The invention provides a genetically engineered bacterium for producing bacterial cellulose as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of bioengineering. According to the invention, a K.medellensis expression vector is constructed, a glycerol transporter expression vector and a glycerol kinase expression vector are further constructed based on the K.medellensis expression vector, and the K.medellensis glycerol transporter expression vector and the glycerol kinase expression vector are transformed into K.medellensis to construct a genetically engineered bacterium for producing bacterial cellulose; glycerin and corn steep liquor are directly used as a carbon source and a nitrogen source respectively, and the fermentation conditions of the genetically engineered bacteria are further optimized, so that environment-friendly and low-cost synthesis of the bacterial cellulose is realized, the yield of the bacterial cellulose is remarkably increased, and the method has good practicability.

The present invention provides an essence containing chlorella extract and a preparation method thereof. The chlorella extract process comprises the following steps: raw material pulverization; enzymatic hydrolysis with cellulase and trypsin; primary fermentation using a mixture of Bacillus natto and Bacillus pumilus; addition of bamboo leaf extract, corn steep liquor, and sucrose, followed by inoculation with Rhodotorula glutinosus for secondary fermentation to obtain the chlorella extract. The mixed fermentation of multiple strains exhibits a synergistic effect, resulting in greater enrichment of natural products during the fermentation process compared to single-strain fermentation. Furthermore, the present invention provides an essence containing chlorella extract, glycerin, sodium hyaluronate, honey, and water, which exhibits significant moisturizing, antioxidant, and soothing effects.

The utility model relates to a system for preparing high-purity inositol using corn slurry, the system comprising a precipitation tank, a first plate-frame filter, a fitin dissolving tank, an enzymolysis tank, a neutralization tank, a second plate-frame filter, a nanofiltration system, a first evaporator, a decolorizing tank, an ion exchange column, a second evaporator, a first crystallization kettle, a first centrifuge, a crude product dissolving tank, a second crystallization kettle, a second centrifuge, a hot air drying tank and a cold air fluidized bed connected sequentially by pipelines, a feed inlet of corn supernatant and a feed inlet of lime milk suspension are respectively arranged on the precipitation tank, a feed inlet of dilute hydrochloric acid solution is arranged on the fitin dissolving tank, and the material coming out of the discharge end of the cold air fluidized bed is the required inositol crystal product. The utility model can quickly obtain enzymolysis raw material liquid phytic acid, and compared with calcium phytate suspension, the homogeneity of the liquid phytic acid enzymolysis process system is improved, the enzymolysis conversion rate is increased to more than 98%, and the purity of the inositol crystal product is increased to more than 99.5%.

An object of the present invention is to provide a lactase solution showing good permeation through a filtration filter, a lactase solution showing good permeation through a filtration filter and having a good residual lactase activity, and a lactase solution showing good permeation through a filtration filter and having a good residual lactase activity even when a lactase solution having a low protease activity and a low arylsulfatase activity as contaminant enzymes is used.A lactase solution containing any of (i) to (iii) below.(i) 0.0001 to 0.1 mass % of an unsaturated fatty acid or a salt thereof(ii) 0.0001 to 0.1 mass % of a saturated fatty acid salt(iii) 0.01 to 10 mass % of at least one selected from yeast extract, soybean peptone, pea protein, a casein degradation product, and corn steep liquor

The invention belongs to the technical field of food and medicine, and relates to application of corn germ concentrated powder in improvement of osteoarthritis. The invention particularly relates to application of corn germ concentrated powder, namely corn antioxidant multienzyme, in preparation of medicines or health-care foods for treating rheumatoid arthritis and osteoporosis. In the corn germ concentrated powder, superoxide dismutase / (U / g) is greater than or equal to 8500, glutathione peroxidase / (U / g) is greater than or equal to 5000, and catalase / (U / g) is greater than or equal to 750. The corn germ concentrated powder can also be prepared by the following method: cleaning corn, germinating, adding water into the germinated corn, grinding and pulping, adding a settling agent into the corn steep liquor, standing, taking supernate, adding part of sodium ascorbate, performing temperature-controlled concentration, adding the rest sodium ascorbate and a stabilizer into the concentrated solution, and uniformly mixing to obtain the corn germ concentrated powder. And drying at controlled temperature, crushing, and mixing with a proper amount of silicon dioxide. The prepared corn germ concentrated powder has the effect of resisting rheumatoid arthritis.

The invention discloses a preparation method of a compound microbial fertilizer, and belongs to the technical field of microbial fertilizers, the preparation method comprises the steps of starch gelatinization, strain activation, shake-flask culture, immobilization, fermentation and material mixing; the immobilization comprises the following steps: centrifuging a paenibacillus polymyxa shake-flask culture, and collecting wet thalli; mixing the wet thalli with normal saline, stirring at room temperature, adding the gelatinized starch solution, and continuously stirring to obtain a mixed bacterial solution; stirring a calcium chloride aqueous solution at 30-35 DEG C, dropwise adding the mixed bacteria solution, continuously stirring after dropwise adding, filtering, and taking filter residues to obtain immobilized paenibacillus polymyxa; the compound microbial fertilizer prepared by the invention is high in viable count, and the viable count is slightly influenced by the content of each substance in the corn steep liquor.

The invention relates to a method for deep processing of corn by using a pressurized soaking method, in particular to a method for preparing dextrin, syrup or sugar, in which the pressurized soaking method is used for replacing a soaking process of a traditional wet corn processing method, so that the corn quickly absorbs water and swells on the premise of not damaging the quality of germs and endosperm and not discharging waste water and waste gas, and the dextrin, the syrup or the sugar is prepared. The process requirements of a wet corn processing method are met, germs and corn husks are further removed, and the obtained germs and corn husks are refined, so that the germs and the corn husks respectively reach commercial selling standards; directly mixing and liquefying the corn slurry without germs and corn husks; the resulting liquefied liquid may be dried to produce dextrins, or further saccharified, refined, concentrated to produce syrup, or optionally further produce sugars.

The utility model discloses a corn germ meal slurry adding and drying system which is characterized in that germ meal in a leaching workshop enters a vibrating screen, an oversize outlet is connected with a lower end inlet of a first elevator, and an undersize outlet is connected with a germ meal packaging unit through a small particle discharging conveyor; an outlet in the tail end of the small particle discharging conveyor is also connected with an inlet in the lower end of the first elevator, the upper end of the first elevator is connected with an inlet of the surge bin, an outlet of the surge bin and the corn steep liquor pipe are both connected with the slurry adding mixer, and a discharging port of the slurry adding mixer is connected with the standing bed; a discharging port of the standing bed is connected with an inlet of the belt conveyor through the discharging machine, an outlet of the belt conveyor is connected with the roller dryer, an outlet of the roller dryer is connected with an inlet of the tube bundle dryer, an outlet of the tube bundle dryer is connected with the cooler through the second elevator, and a discharging port of the cooler is connected with an inlet of the smashing machine. And an outlet of the crusher is connected with the pulp adding and packaging unit. The system can improve the added value of products and increase the profit of enterprises.

The invention provides a method for producing succinic acid by immobilized cells, and belongs to the technical field of fermentation. The fermentation culture medium comprises the following components: 50 to 54 g / L of glucose, 8 to 10 g / L of corn steep liquor, 5.7 to 5.9 g / L of monopotassium phosphate, 6.3 to 6.5 g / L of dipotassium phosphate, 0.17 to 0.22 mg / L of calcium chloride, 1.67 to 1.73 mg / L of cobalt chloride hexahydrate, 0.46 to 0.50 mg / L of zinc sulfate heptahydrate, 1.6 to 1.8 mg / L of biotin, 6.0 to 6.5 mg / L of nicotinamide, 8.2 to 8.5 mg / L of vitamin B5 and 0.32 to 0.34 g / L of an active agent. By adopting the method disclosed by the invention, the succinic acid production rate is high, the malic acid content is low, the immobilized microspheres can be repeatedly fermented for multiple times, and the succinic acid content of a single batch is stable and is always kept at a relatively high level.

This invention relates to the fields of agricultural product processing and bio-feed technology, specifically to a method and application for detoxifying corn steep liquor. The method includes: subjecting a mixture of corn steep liquor, a composite color-protecting agent, and a composite catalyst to a first reaction; allowing the reaction system after the first reaction to stand in a mixture with a composite adsorbent; subjecting the mixture containing the reaction system after the stand-in reaction and a composite enzyme preparation to stand-in treatment, mixing treatment, and enzymatic hydrolysis; and subjecting the mixture containing the reaction system after the enzymatic hydrolysis and an activated composite microbial agent to anaerobic fermentation. This method employs a multi-stage chemical-enzyme-fermentation linkage process, achieving efficient detoxification of corn steep liquor while significantly improving flavor and nutrition. This method synergistically removes mycotoxins and sulfites, and enhances palatability and probiotic value through fermentation transformation of off-flavor substances and enrichment of small peptides and lactic acid. The process is simple and requires no adsorbent separation.

The invention provides an auxin formula for promoting early germination of Chinese cabbage, which is characterized by comprising corn steep liquor, brown sugar, clear water and the like, the final germination rate of Chinese cabbage seeds in clear water and plant auxin is the same, but the third day is the first day of seed germination, and the fourth day is the second day of seed germination. The germination rate of the seeds in the auxin is higher than that in clear water, which indicates that the auxin can stimulate the seeds to break dormancy and germinate in advance.

The invention discloses a genetically engineered bacterium and method for producing succinic acid by fermenting corn straw hydrolysate, and the method comprises the following specific steps: 1, preparing the corn straw hydrolysate: treating 1-2% NaOH solution at 110-130 DEG C for 0.5-1.5 h, with the lignin removal rate being greater than or equal to 70% and the cellulose retention rate being greater than or equal to 85%; step 2, aerobic fermentation of the genetically engineered bacteria: a culture medium contains 8-12 g / L of glucose and 3-7 g / L of yeast extract or corn steep liquor, and the genetically engineered bacteria are cultured at 35-39 DEG C and 180-220 rpm until OD600 is 2.5-3.5; and 3, anaerobic fermentation of the bacterial strain: taking the corn straw hydrolysate obtained in the step 1 as a carbon source, and adding a buffering agent into the genetically engineered bacteria for anaerobic fermentation. According to the recombinant escherichia coli E.coli SUCC-01, succinic acid is produced by knocking out an acetic acid synthesis gene pta / ackA and overexpressing an sdhA / mdh gene and combining with corn straw hydrolysate through fermentation, the yield of the succinic acid can reach 80 g / L, the total sugar utilization rate is larger than or equal to 90%, the by-product acetic acid is smaller than or equal to 12 g / L, and the yield is increased by 60% compared with that of a wild type strain.

The application provides corn steep liquor for fermentation and a preparation method and application thereof, and belongs to the technical field of biology.The preparation of the corn steep liquor for fermentation comprises the following steps: hydrolyzing corn steep liquor, homogenizing the hydrolyzed corn steep liquor, and then heating and filtering the homogenized corn steep liquor to obtain the corn steep liquor for fermentation.The hydrolysis and high-pressure homogenization process are combined, microorganisms remaining in the corn steep liquor can be effectively cracked, macromolecular proteins and other substances can be decomposed into nutrients which are easy to utilize, the risk of contamination of a fermentation tank due to incomplete sterilization of the corn steep liquor is reduced, and the conversion rate of a fermentation product is improved.

The invention belongs to the technical field of feed biology, and particularly relates to an aflatoxin oxidase mutant as well as a coding gene and application thereof. According to the three-point combined mutant E188R-S210G-T418A provided by the invention, the enzyme activity is 177% of that of a female parent BlCotA; after water bath is carried out for 30 minutes at 80 DEG C, the enzyme activity is 68% of the initial enzyme activity, and the thermal stability is superior to that of a female parent BlCotA; after the mutant E188R-S210 G-T418A is used for treating raw milk for 1 hour, 2 hours and 4 hours, the residual quantity of the AFM1 is respectively reduced to 0.31 ng / mL, 0.12 ng / mL and 0.01 ng / mL; after corn steep liquor is treated by the mutant E188R-S210G-T418A, the degradation rates of AFB1, AFB2, AFG1 and AFG2 are respectively 93.71 percent, 93.95 percent, 96.79 percent and 97.58 percent.

The invention relates to the technical field of microbial oil recovery, in particular to bacillus subtilis 20P3 LZT, fermentation liquor and application of the bacillus subtilis 20P3 LZT to microbial oil recovery, the bacillus subtilis 20P3 LZT is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30366. The bacillus subtilis 20P3 LZT is found for the first time, can generate surfactant lipopeptide, can grow on the basis of molasses or corn steep liquor dry powder as a carbon source, has good emulsification and viscosity reduction performance on crude oil, has the emulsification and viscosity reduction rate of 70% on thick oil, can reduce the viscosity of the crude oil and enhance the fluidity of the crude oil, and can be used for preparing crude oil. On one hand, the oil-water fluidity ratio is reduced, and on the other hand, the emulsified crude oil has the effects of profile control and liquid flow diversion, so that the crude oil recovery rate is indirectly improved, and a new strain and a material are provided for a microbial oil recovery technology.

The invention relates to the technical field of fertilizers, in particular to a microbial amino acid-containing water-soluble fertilizer and a preparation method thereof. The method comprises the following steps: providing a first corn steep liquor and a second corn steep liquor; adding a Trichoderma harzianum-Purpureocillium lilacinum complex microbial inoculant and lactic acid bacteria into the first corn steep liquor, and carrying out first fermentation to obtain a first fermentation broth; mixing the first fermentation liquor with the second corn steep liquor to obtain mixed corn steep liquor; adding bacillus subtilis into the mixed corn steep liquor, and carrying out second fermentation to obtain second fermentation liquor; and sequentially adding a nitrogen fertilizer, a phosphate fertilizer and a potash fertilizer into the second fermentation liquor to obtain the water-soluble fertilizer containing microbial amino acids. The microbial amino acid-containing water-soluble fertilizer prepared by the preparation method provided by the invention can effectively improve the yield and quality of crops, improve the soil structure, enhance the soil fertility, promote the root development of the crops and improve the resistance of the crops to adversity.

The invention belongs to the technical field of microbial fermentation, and provides a plant lactobacillus fermentation process. The fermentation process provided by the invention comprises the preparation processes of strain preparation, primary seed tank culture, fermentation tank culture and the like. According to the fermentation process, low-cost raw materials such as corn steep liquor and molasses are used as a culture medium, a trace amount of inorganic salt is added into the culture medium, the ratio of all the raw materials is adjusted, and a material supplementing process is combined, so that the cost is reduced, the growth of thalli and stable fermentation of the thalli are ensured, and the zymophyte quantity of the plant lactic acid bacteria is increased to 2,59 billion from original 10,000,000,000,000,000,000,000,000,000,000,000,000,000.

The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor, and belongs to the technical field of corn deep processing and detoxification. The invention discloses a method for efficiently degrading mycotoxin in corn steep liquor. The method comprises the following steps: adjusting the pH value of thick corn steep liquor by using an alkaline solution; adding a solid catalyst into the corn steep liquor; carrying out detoxification treatment by combining heterogeneous ozone oxidation reaction with microbubbles; and separating and recovering the solid catalyst to obtain the detoxified corn steep liquor. The vomitoxin content of the treated concentrated corn pulp is smaller than 1000 g / kg, the degradation rate is larger than 93%, the zearalenone toxin content is smaller than 33 g / kg, and most of the zearalenone toxin is removed. According to the method disclosed by the invention, the content of fungaltoxin in the corn steep liquor is reduced, so that the economic value of corn byproducts is improved, the economic benefit of grain trade is favorably improved, and the storage period and value of produced products are improved.

The present invention belongs to the technical field of zearalenone extraction and relates to a method for extracting zearalenone from corn steep liquor to prepare a crude standard. The method comprises centrifuging corn steep liquor containing ZEN to obtain a supernatant; treating the supernatant using a PA66 membrane to obtain a PA66 membrane enriched with ZEN; eluting the PA66 membrane enriched with ZEN to obtain an eluate; and purifying the eluate by rotary evaporation to obtain a crude ZEN standard. The PA66 membrane is used to enrich and extract ZEN from ZEN-contaminated corn byproducts, achieving an enrichment and extraction efficiency exceeding 90%, providing a reliable and effective method for the high-value utilization of corn byproducts. The PA66 membrane is used to efficiently and selectively adsorb and enrich ZEN to initially prepare a crude ZEN standard, providing a new approach to the preparation of ZEN standard products.

The invention relates to a preparation method of a corn steep liquor fertilizer, in particular to a preparation method of a corn steep liquor fertilizer based on circulating membrane separation enhanced enzymolysis. In order to solve the problem that the existing method for preparing the fertilizer from the corn steep liquor is extensive, the method comprises the following steps: 1, diluting the corn steep liquor, adding a biological flocculant, adding enzyme, and separating by a circulating membrane to respectively obtain filtrate 1 and concentrated liquor 1; adding enzyme into the filtrate 1, and separating by a circulating membrane to respectively obtain filtrate 2 and concentrated liquid 2; adding charcoal and a compound material into the concentrated liquid 1, uniformly stirring, drying, extruding and granulating to obtain a solid fertilizer; and adding the concentrated liquid 2 into the compound material to obtain the liquid fertilizer. No waste liquid or waste is generated in the whole process, the large-molecular-weight low-solubility slow-degradation solid fertilizer and the small-molecular-weight high-solubility easily-absorbable liquid fertilizer are prepared according to different absorption characteristics, and the method is applied to the field of fertilizer preparation.

The application provides application of neutral protease in degradation of fumonisin, and belongs to the technical field of biology, and it is verified through experiments that the neutral protease has good degradation effect on FB1. The application also provides a compound enzyme preparation which comprises the following components: 1-5% neutral protease, 5-20% montmorillonite, 75-94% soluble starch, and only by adding 0.5wt%-1.5wt% of the compound enzyme preparation, the efficient degradation of FB1 in common contaminated feed can be realized; specifically, the degradation rate of the compound enzyme preparation on FB1 in corn flour can reach 95.73%, the degradation rate of the compound enzyme preparation on FB1 in DDGS can reach 96.43%, the degradation rate of the compound enzyme preparation on FB1 in corn steep liquor can reach 64.74%, and the degradation rate of the compound enzyme preparation on FB1 in corn cob powder can reach 93.74%.

The invention relates to the technical field of microorganisms, in particular to Devodia sp. And a method for fermenting corn steep liquor by using the Devodia sp. The invention provides the Deworth's bacteria capable of efficiently degrading vomitoxin in the corn steep liquor, the Candida krusei and the Candida krusei are used for cooperatively fermenting the corn steep liquor, the pH of the corn steep liquor can be increased, the content of vomitoxin and antinutritional factors in the corn steep liquor can be reduced, the content of choline in the corn steep liquor is remarkably increased, the quality of the corn steep liquor is obviously improved, and the yield of the corn steep liquor is increased. The corn steep liquor subjected to synergistic fermentation can be better applied to the field of animal feed and has a wide application prospect.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a digestible biological protein fermentation material. According to the fermentation method, candida utilis serves as a fermentation strain, industrial and agricultural waste glucose mother liquor and corn steep liquor serve as substrates, high-yield mycoprotein is obtained under the relative low-temperature fermentation condition of 20-25 DEG C, and the obtained fermentation liquor is subjected to single enzymolysis, so that a fermentation material easier to digest is obtained. When weaned piglets are fed with the obtained fermented material, the diarrhea rate of the piglets can be effectively reduced.

The invention relates to the technical field of biological fermentation. The invention provides a strain of Geotrichum candidum Do-Ya-2-12, and the preservation number of the strain of Geotrichum candidum Do-Ya-2-12 is CGMCC (China General Microbiological Culture Collection Center) No. 33104. The strain of the geotrichum candidum Do-Ya-2-12 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the strain is CGMCC No.33104, the preservation time of the strain is December 17, 2024, the preservation center is called CGMCC for short, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain provided by the invention can replace a conventionally used carbon source and nitrogen source with a corn soaking solution or corn steep liquor, is environment-friendly, improves the conversion rate of lactic acid by microorganisms such as yeast, reduces the production cost, simplifies the production process, and realizes efficient output of single-cell protein.

The invention discloses recombinant klebsiella pneumoniae with high yield of 3-hydracrylic acid as well as a construction method and application of the recombinant klebsiella pneumoniae, and belongs to the technical field of genetic engineering. The construction method comprises the following steps: by taking klebsiella pneumoniae as an original strain, introducing kgsadh genes for coding alpha-ketoglutarate semialdehyde dehydrogenase, and knocking out 1, 2, 3, 4-tetramethyl-3-oxoglutarate; the method comprises the following steps: constructing recombinant klebsiella pneumoniae for high yield of 3-hydracrylic acid by using one or more of a dhaT gene of 1, 3-propylene glycol dehydrogenase, an ldh gene for coding L-lactic dehydrogenase, an ldhA gene for coding 2-hydracrylic acid dehydrogenase and a yqhD gene for coding alcohol dehydrogenase. Compared with an initial strain and a recombinant strain, the 3-HP of the recombinant strain is remarkably improved, and the yield of the 3-HP is the highest when an organic nitrogen source is determined to be corn steep liquor or the concentration of glycerol is controlled to be 15-25g / L through organic nitrogen source optimization and glycerol concentration control, so that a new strain choice is provided for high yield of the 3-hydracrylic acid.

The invention relates to a corn steep liquor dry powder production process and equipment. The production process comprises the following steps: 1, a corn steep liquor production process: (1) raw material pretreatment, (2) soaking process, and (3) soaking liquid separation; 2, concentrating the corn steep liquor; and 3, drying the concentrated corn steep liquor. The production equipment comprises a low-temperature vacuum concentrator, an evaporation concentration system with a heat pump system and a vacuum liquid belt dryer. The corn steep liquor concentration efficiency is high, the concentration effect is good, energy consumption is low, waste gas pollution is avoided, active ingredients of the corn steep liquor are well kept, and the product quality is excellent.

The application provides application of neutral protease in degradation of fumonisin, and belongs to the technical field of biology, and it is verified through experiments that the neutral protease has good degradation effect on FB1. The application also provides a compound enzyme preparation which comprises the following components: 1-5% neutral protease, 5-20% montmorillonite, 75-94% soluble starch, and only by adding 0.5wt%-1.5wt% of the compound enzyme preparation, the efficient degradation of FB1 in common contaminated feed can be realized; specifically, the degradation rate of the compound enzyme preparation on FB1 in corn flour can reach 95.73%, the degradation rate of the compound enzyme preparation on FB1 in DDGS can reach 96.43%, the degradation rate of the compound enzyme preparation on FB1 in corn steep liquor can reach 64.74%, and the degradation rate of the compound enzyme preparation on FB1 in corn cob powder can reach 93.74%.