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Strain of gluconacetobacter and application thereof

A technology of gluconacetobacter and strains, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of high production cost, application limitation, low bacterial cellulose production, etc., and achieve good water absorption and high reliability Regulatory, suitable for the effect of large-scale industrial fermentation production

Inactive Publication Date: 2009-12-02
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the physical and chemical properties and mechanical properties of bacterial cellulose are superior to those of plant cellulose, and it has been proved that it has a wide range of commercial application prospects in industrial production, its application is limited due to the low yield and high production cost of bacterial cellulose.

Method used

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  • Strain of gluconacetobacter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Isolation of Gluconacetobacter sp.SC-01 (Gluconacetobacter sp.SC-01)

[0031] The experimental materials come from rotten persimmons produced in Ji County, Tianjin.

[0032] The specific steps are as follows: take the rotten persimmon juice, filter it with a 100-mesh sieve, and dilute it in a 10-fold gradient with sterile normal saline, and dilute it to 10 1 -10 6 , take 10 3 -10 5 Spread it on a solid GYC medium plate, culture at 25-35°C for 4 days, observe the growth of the colony, pick the colony with transparent spots and transfer it to the liquid HS medium for 10 days, and there is a film on the surface of the culture medium After the membrane is treated, it is detected as a bacterial cellulose membrane, and it is preliminarily judged that the bacteria is a bacteria that produces bacterial cellulose. After Gram staining, physiological and biochemical experiments, 16S rDNA gene sequence analysis and BIOLOG identification, it was determined that the st...

Embodiment 2

[0033] Embodiment 2, fermentation of Gluconacetobacter sp.SC-01 (Gluconacetobacter sp.SC-01)

[0034] Fermentation medium 1 was used. The specific steps are as follows: first inoculate the strains into the seed culture medium, shake the shaker at 25-35°C, and the rotation speed is 135rpm. After 24 hours of cultivation, insert the fermentation medium at an inoculum size of 10%, and shake fully during inoculation, so that The cells are evenly dispersed, and cultured at a constant temperature of 25-35°C for 10 days. Take out the cellulose membrane, rinse with deionized water several times to remove the medium and impurities on the surface of the membrane, immerse the membrane in 0.1mol / L NaOH, boil at 100°C for 1 hour, remove the bacterial cells and residual medium in the membrane, Milky white and translucent, soak in deionized water overnight, then rinse with deionized water several times until the pH is about 7.2, then centrifuge in a 250ml centrifuge tube at 4000rpm for 40min...

Embodiment 3

[0035] Embodiment 3, adopt fermentation medium 2. The specific steps are as follows: first inoculate the strains into the seed culture medium, shake the shaker at 25-35°C, and the rotation speed is 135rpm. After 24 hours of cultivation, insert the fermentation medium at an inoculum size of 10%, and shake fully during inoculation, so that The cells are evenly dispersed and cultured with constant temperature shaking at 25-35°C for 5 days. The following steps are the same as in Example 2.

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Abstract

The invention discloses a strain of gluconacetobacter SC-01 (Gluconacetobacter sp.SC-01) and application thereof and relates to the strain of the gluconacetobacter and a method for producing bacterial cellulose by utilizing the gluconacetobacter. The strain is inoculated into a seed culture medium and subjected to shaking culture by a shaker for 24 hours at a temperature of between 25 and 35 DEG C; and the strain with 10 percent of inoculation amount is inoculated into a fermentation medium, stood at the constant temperature of between 25 and 35 DEG C and cultured for 10 days or subjected to shaking culture for 5 days. The cellulose is separated, purified and dried. A carbon source of the culture medium is one or more of glucose, mannitol, sucrose, galactose, fructose or xylose; and a nitrogen source is one or more of yeast powder, peptone or dry powder of corn steep liquor. The strain still has high yield of the bacterial cellulose in low pH environment and is suitable for large-scale industrialized fermenting production. The bacterial cellulose obtained by the gluconacetobacter has the characteristics of ultra-pureness, ultra-fineness, high crystallization degree, strong hygroscopicity, degradability, high mechanical strength and the like.

Description

technical field [0001] The invention relates to a strain of Gluconacetobacter sp.SC-01 and its application, in particular to a fermentation method of a strain of Gluconacetobacter sp.SC-01 and the use of the A method for the production of bacterial cellulose by bacteria. Background technique [0002] In order to distinguish the cellulose derived from plants, the cellulose derived from microorganisms is called bacterial cellulose (bacterial cellulose, BC). According to reports, bacteria of the genus Gluconacetobacter have the strongest ability to synthesize bacterial cellulose and have the potential for large-scale fermentation production. [0003] Compared with plant cellulose, bacterial cellulose is an ultrafine fiber network composed of ultrafine fibers, and its ultrafine fiber diameter is only 1 / 100 of that of plant fibers. Its elastic modulus is as high as 1.5×10 10 Pa, equivalent to aluminum. Bacterial cellulose is a biodegradable polymer with many unique properties...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
Inventor 宋存江苏文萍曹名锋孔梅梅李保宾王淑芳
Owner NANKAI UNIV
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