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Novel bacteriological culture medium

A medium, biotransformation technology, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., which can solve problems such as use

Inactive Publication Date: 2010-03-03
TIANJIN JINYAO GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] This patent mainly solves the problem caused by the use of molasses in the microbial culture medium in the process of obtaining AD and ADD by phytosterol fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-a

[0037] Species: Mycobacterium: Mycobacterium fortuitum. NRRL B-8153.

[0038] 10 different batches of corn steep liquor were fermented according to the quantity and process in this example. Except corn steep liquor, other materials are the same batch of materials.

[0039] Seed culture stage:

[0040] Seed medium (per liter):

[0041] 40ml corn syrup

[0042] 5.2g NaNO 3 ,

[0043] 0.8g NH 4 h 2 PO 4

[0044] 5.3g glucose

[0045] The rest is sterile water

[0046] Medium volume: 200ml / flask

[0047] Inoculum volume: 3ml of cell suspension from agar slant per 200ml of new medium

[0048] Container: 1000ml conical flask

[0049] Stirring: Shaker (1”throw) 200rpm

[0050] Temperature: 30°C

[0051] Growth time: 72 hours culture

[0052] Note: A good culture organism is indicated if a yellow cell pellet forms at the bottom

[0053] method:

[0054] Inoculum for biotransformation was prepared in culture medium. Inoculate Erlenmeyer flasks from the agar surface (...

Embodiment 1-b

[0073] The culture medium in this example was 10 different batches of Refiner molasses instead of corn steep liquor, and fermentation was carried out according to the ratio and process of the bacteria and culture medium in Example 1-a. All other materials except corn steep liquor are the same batch of materials as in Example 1-a.

[0074] Table 2

[0075]

[0076] Note: Lot numbers in Table 2 refer to different Refiner molasses lot numbers. The content refers to the total content of AD and ADD obtained by fermenting phytosterols using the Refiner molasses as a medium.

[0077] In this embodiment, 10 products of different batch numbers are used for Refiner molasses, and 10 products of different batch numbers are also used for corn steep liquor.

Embodiment 2-a

[0080] Species: Mycobacterium: Mycobacterium sp NRRL-B 3683

[0081] The corn steep liquor of 10 different batches was fermented according to the quantity and process in this example. Except corn steep liquor, other materials are the same batch of materials.

[0082] Seed culture stage:

[0083] Seed medium (per liter):

[0084] 10ml corn syrup

[0085] 8.7g NaNO 3 ,

[0086] 0.3g NH 4 h 2 PO 4

[0087] 15g glucose

[0088] Na 2 HPO 4 and NaH 2 PO 4 Adjust the pH of the buffer to 7.0

[0089] The rest is sterile water

[0090] Medium volume: 200ml / flask

[0091] Inoculum volume: 3ml of cell suspension from agar slant per 200ml of new medium

[0092] Container: 1000ml conical flask

[0093] Stirring: Shaker (1”throw) 200rpm

[0094] Temperature: 30°C

[0095] Growth time: 72 hours culture

[0096] Steroid fermentation medium (per liter):

[0097] 10ml corn syrup

[0098] 8.7g NaNO 3 ,

[0099] 0.6g NH 4 h 2 PO 4

[0100] Na 2 HPO 4 and NaH 2 PO 4 ...

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Abstract

The invention provides a novel bacteriological culture medium, which is used in a fermenting process for converting phytosterin into AD and ADD by adopting athrobacter nicotinovorans, mycobacteria ornocardia, contains corn steep liquor and inorganic salt, and contains 5 to 50ml of the corn steep liquor per liter preferably. The culture medium can improve the conversion ratio of biofermentation.

Description

field of invention [0001] The present invention relates generally to the formulation of a bacterial culture medium, in particular a culture medium for proliferating microorganisms of the genus Mycobacterium for the conversion of phytosterols to androstenediones. Background technique: [0002] Androst-4-ene-3, 17-dione (androst-4-ene-3, 17-dione, referred to as androstenedione or AD) is an irreplaceable intermediate of steroid hormone drugs, which plays an important role in the body. very important regulation. It can be said that almost all steroid hormone drugs are produced with AD as the starting material. Such as for the production of sex hormones, progesterone, anabolic hormones and corticosteroids, but also for the synthesis of more than 100 kinds of drugs such as hydrocortisone, prednisone oxide, progesterone, estrenol, dexamethasone, etc. It is an essential raw material for the production of anti-pregnancy drug mifepristone and various family planning drugs. AD is n...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P33/00C12R1/32C12R1/01
Inventor 李静李金禄孙亮
Owner TIANJIN JINYAO GRP
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