75 results about "Mycobacterium sp. e" patented technology

The present invention relates to methods and compns. for treating nucleic acids, and in particular, methods and compns. for the detection and characterization of nucleic acid sequences and sequence changes. The invention provides methods for examg. the conformations assumed by single strands of nucleic acid, forming the basis of novel methods of detection of specific nucleic acid sequences. The present invention contemplates use of novel detection methods for, among other uses, clinical diagnostic purposes, including but not limited to the detection and identification of pathogenic organisms. Examples are presented for the analysis of Mycobacterium tuberculosis and hepatitis C virus genes.

The present invention relates generally to nucleic acid and amino acid sequences of a fusion polypeptide comprising a Mycobacterium tuberculosis polypeptide, and a heterologous polypeptide of interest, expression vectors and host cells comprising such nucleic acids, and methods for producing such fusion polypeptides. In particular, the invention relates to materials and methods of using such M. tuberculosis sequence as a fusion partner to facilitate the stable and high yield expression of recombinant heterologous polypeptides of both eukaryotic and prokaryotic origin.

The invention relates to a rapid detecting technology for detecting mycobacterium tuberculosis through nucleic acid constant-temperature amplification and a kit for qualitative detection. The kit comprises an instrument-free DNA (Deoxyribonucleic Acid) extraction kit, a mycobacterium tuberculosis nucleic acid constant-temperature amplification reaction liquid, a contamination-prevention nucleic acid amplification product detecting device, mycobacterium tuberculosis positive control, and mycobacterium tuberculosis negative control. The kit disclosed by the invention has the advantages of high specificity, high sensitivity and high reaction speed, wherein 1-1.5 hours are only needed from the step of processing a single sample to the step of completing the detection; and the kit can simultaneously meet requirements on sample detection of medium flux and low flux and is particularly suitable for being used in field detection and primary hospitals, and one constant-temperature instrument is only needed in the entire reaction process.

The invention provides a novel bacteriological culture medium, which is used in a fermenting process for converting phytosterin into AD and ADD by adopting athrobacter nicotinovorans, mycobacteria ornocardia, contains corn steep liquor and inorganic salt, and contains 5 to 50ml of the corn steep liquor per liter preferably. The culture medium can improve the conversion ratio of biofermentation.

Compounds and methods for diagnosing tuberculosis are disclosed. The compounds provided include polypeptides that contain at least one antigenic portion of one or more M. tuberculosis proteins, and DNA sequences encoding such polypeptides. Diagnostic kits containing such polypeptides or DNA sequences and a suitable detection reagent may be used for the detection of M. tuberculosis infection in patients and biological samples. Antibodies directed against such polypeptides are also provided.

The present application is to provide a composition and method for stabilizing and maintaining the viability of hardy microorganisms from sample collection to downstream analysis. In particular, there is a method for preserving viable hardy bacteria, such as Mycobacteria, Bacillus anthracis, or Clostridium difficile, in a biological sample, comprising contacting the biological sample with a stabilization composition, wherein the stabilization composition comprises a chelating agent, a denaturing, a salt and has a pH between about 6 and about 11.

The invention provides a method for producing 9-alpha-hydroxyandrostenedione by microbial fermentation. Recombinant Mycobacterium smegmatis is fermented to convert phytosterin to produce the 9-alpha-hydroxyandrostenedione. The method comprises the following steps: constructing a recombinant expression vector pMV261-ksh; constructing a recombinant strain; and carrying out culture propagation and fermentation conversion. Gene engineering means are utilized to obtain the gene engineering strain for overexpressing 3-sterone-9-alpha-hydroxylase gene (ksh); after the strain is fermented for 120 hours, the conversion rate of the phytosterin is up to 95% above; and the method has the characteristics of high yield, fewer byproducts, short fermentation time simple extraction, small pollution, low pressure for environmental protection and the like, and has huge advantages in industrial production.

PCT No. PCT/DK97/00341 Sec. 371 Date Dec. 29, 1997 Sec. 102(e) Date Dec. 29, 1997 PCT Filed Aug. 22, 1997 PCT Pub. No. WO98/10079 PCT Pub. Date Mar. 12, 1998Expression vectors capable of being replicated in lactic acid bacterial cells comprising a promoter region comprising (a) a promoter sequence element the function of which is regulatable by an environmental or growth condition factor and (b) at least one further nucleotide sequence element, the position, orientation, presence and/or sequence of which element has a regulatory effect on the expression of a gene operably linked to the promoter region in which vectors the position, orientation, presence and/or sequence of at least one of said elements (a) or (b) is modified relative to the position, orientation, presence and/or sequence of the corresponding non-modified element whereby the expression of the gene is altered, and a lactic acid bacterium which is transformed with such a vector as defined above are provided. The recombinant cells containing such a regulatable or inducible gene expression system are useful as food or feed starter cultures or as strains for the production of gene products such as pharmaceutically or immunologically active compounds including oligo- or polypeptides derived from a Mycobacterium species including M. tuberculosis.

The invention belongs to the technical field of biological catalyst, and particularly relates to a mycobacteria genetically engineered bacterium with cofactor regeneration function and its applicationin fermentation of two liquid phases. Through a NADH oxidizing system in the cell, the genetically engineered bacterium for transforming plant sterol biology is structured and transformed for the NADH oxidizing system in the cell, thus the problem of low transforming efficiency of androstenedione in the oil water two-liquid-phase fermenting system is solved; the genetically engineered bacterium also provides possibility for the wide application of the oil water two-liquid-phase fermenting system in industrial production of the androstenedione. The genetically engineered bacterium MNR M3N2 structured by the invention can improve the AD (D) output by 1.4-2.9 times during the preparation process of androstenedione by degrading the phytosterol through the oil water two-liquid-phase fermentingsystem fermenting method.

The invention provides a mycobacterium cosmeticum byf-4 with the capacity of degrading a benzene compound and an application thereof. The mycobacterium cosmeticum byf-4 is stored in Chinese typical culture storage center which has an address: Wuhan University Wuhan China, 430074, a storage date: Oct. 29, 2008 and a storage number: CCTCC No. M 208180. The invention has the advantages that the strain can rapidly degrade various benzene compounds with high efficiency and has an important meaning to the engineering practice of biologically purifying contaminants caused by the benzene compounds and the derivatives thereof.

The present invention discloses a preparation method of a new type incubation medium, belonging to the medical inspection field. The method can promote growth of mycobacteria. The incubation medium comprises a solid part and a liquid part: the solid part is the modified Roche's incubation medium that is generally used at home and abroad and the liquid part consists of basic incubation medium, growth promoter and bacteria inhibitor. The incubation medium can not only promote growth of mycobacteria, but also inhibit other bacteria. The growing mycobacteria can form violet red bacteria colony on the solid inclined plane of incubation medium, which is beneficial to elementary appraisal. The incubation medium can shorten incubation time obviously, increase incubation positive rate considerably, very suitable for fast inspection of mycobacteria for the hospitals and the sanitation and anti-epidemic institutions.

An object of the present invention is to provide a novel primer and prove for detecting tubercle bacillus capable of avoiding false positive, and an easy-to-use, rapid and high-sensitivity method for detecting human tubercle bacillus (Mycobacterium tuberculosis) using the same. The present invention relates to an oligonucleotide comprising a part of or an entire sequence of the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, or a part of or an entire sequence of the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis, a primer and a probe containing the oligonucleotide for detecting Mycobacterium tuberculosis, and a method for detecting Mycobacterium tuberculosis using the primer and the probe.

The invention discloses a method for producing A ring degradation products through plant sterol fermentation. The method comprises the following steps: (A) preparing a liquid inoculant containing mycobacterium fortuitum; (B) carrying out fermentation culture; (C) extracting and separating so as to obtain the A ring degradation products. The provided fermentation method for preparing A ring degradation products is carried out in an oil-free condition, thus the ring opening of A ring and B ring is effectively inhibited, thus the degradation is inhibited, the yield is increased, and the weight yield can reach 35 to 40%.

The invention discloses an antituberculous drug drug-resistance gene and a screening method thereof. The antituberculous drug drug-resistance gene is pks12, pks5, PPE34, Rv1978, Rv1847, hemK, PPE3, glyA1, PE_PGRS27, PE_PGRS19, Rv1520, Rv1505c, Rv2407, mmuM, vapB17, Rv3727 and Rv3737. The novel candidate drug target gene is provided for a further understanding of drug resistance mechanism of MDR/XDR mycobacterium tuberculosis and the therapy of MDR/XDR-TB.

The invention provides a complex microbial agent capable of efficiently degrading polycyclic aromatic hydrocarbons. The active ingredients include Mycobacterium sp. having a collection number of CGMCCNo. 6531, Acinetobacter sp. having a collection number of CGMCC No. 6532, and Microbacterium sp. having a collection number of CGMCC No. 6530. The complex microbial agent can take the polycyclic aromatic hydrocarbons, such as fluorine, naphthalene, anthracene, acenaphthene, acenaphthylene, dibenzothiophen, carbazole, chrysene, phenanthrene, pyrene, fluoranthene, benzofluoranthrene, benzanthraceneor benzopyrene as the unique carbon source. The complex microbial agent disclosed by the invention is capable of obviously improving the degradation effect of single colony on organic contaminated soil, the fermentation time of three strains is short, the effect is obvious, and the cost is low. Therefore, the complex microbial agent has strong application prospects and popularization value on polycyclic aromatic hydrocarbon heavy contaminated soil and water.

Disclosed are methods for generating mycolic acid bacterial biosensors for particular analytes (especially industrial pollutants) by the use of innovative methods for isolating DNA encoding an inducible promoter which is induced in response to the specific analyte (and/or associated operon proteins), the methods generally comprising the steps of: (a) culturing a source of mycolic acid bacteria in a selective medium containing said specific analyte and being selective for oligotriphic bacteria; (b) identifying mycolic acid bacteria capable of subsisting on said medium, especially those which do not display catabolic repression; (c) extracting DNA from said mycolic acid bacteria; (d) incorporating said DNA into vectors, such as various shuttle vectors; (e) cloning said vector into a suitable host cell (which may be E. coli strain carrying one or more of the mcrABC mrr hsdSRM recA and recO mutations); (f) screening that host cell (or a second host cell which is preferably a corynebacterium) for said inducible promoter. The methods are exemplified by the isolation of the R. corallina ohp operon.