55results about How to "Visualization of test results" patented technology

The invention discloses a device and method for measuring the evenness of an LED area array light source, belongs to the field of LED area array light source measuring, and aims to solve the problems that currently a point selecting testing method is used to measure the LED area array light source, the accuracy of the evenness measuring is hard to guarantee, and a large amount of manpower, material resources and time needs to be consumed when the point selecting testing method is used to adjust the LED area array light source. The device comprises a straight-line motion mechanism, a detecting device, an analog signal collector and a control system. The device has the advantages that the light energy of the LED area array light source in the whole irradiating area can be scanned and detected in a short time, and the device is fast in detection, high in efficiency, accurate in processing result and visual in detecting result.

The invention discloses a kit for detecting genetically modified soybean in the field of test items. The kit for detecting genetically modified soybean comprises a primer combination and a membrane chip. The kit for detecting genetically modified soybean is characterized in that the primer combination is a seven polymerase chain reaction (PCR) primer combinations; each pair of primers comprise Pat, Bar, Cry1Ab, Cry105, EPSPS, P35S and a soybean endogenous gene sequence Lectin; the membrane chip is seven groups of probe sequences, which are orderly fixed on the surface of a support membrane and a group of positive control probe sequences, the probe sequences are respectively a Pat probe, a Bar probe, a Cry1Ab probe, a Cry105 probe, an EPSPS probe, a P35S probe, a Lectin probe and a Positive probe; an asymmetric PCR amplification primer is formed in the primer combination, and is a Tag primer with a biotin marker (biotin) at the 5' end. By adopting the kit for detecting genetically modified soybean, the defects of time and labor waste, and high cost in the prior art are overcome; the provided kit for detecting genetically modified soybean is simple to operate, quick and sensitive, large in detection throughput, visual in detection result and low in cost.

The invention provides a method for detecting hydrogen peroxide based on a bimetallic Co / Mn-MOFs enzyme-like property colorimetric method and belongs to the technical field of environmental monitoring. The method comprises: synthesizing two-dimensional bimetallic Co / Mn-MOFs through a one-step hydrothermal method. Through the synergistic catalysis effects of two metals in the material and a large specific surface area and accessible active sites of the two-dimensional structure, the Co / Mn-MOFs have good enzyme-like activity. When H2O2 exits, the material can promote decomposition of H2O2 so that a hydroxyl radical .OH is produced and the .OH oxidizes a chromogenic substrate TMB into oxTMB and thus the reaction system is changed from colorless to blue. The colorimetric sensing method has a H2O2 linear detection range of 1 to 100 micromoles per liter and a detection limit of 0.85 micromoles per liter. The method has the characteristics of simpleness, visuality, low cost and high sensitivity and provides a novel idea for use of the material in environmental monitoring and biological analysis.

The invention relates to a microbial fluorescent staining solution and an application thereof. The microbial fluorescent staining solution comprises lectin, fluorescein, nucleic acid dye, a buffer solution, an anti-quenching agent, a bacteriostatic agent and water; the microbial fluorescent staining solution can be used in microbial dyeing, specific recognition of agglutinin on glycoprotein and specific staining of nucleic acid by nucleic acid dye are utilized, according to the invention, the fungi are blue, green or red, the bacteria are red, the morphology is combined to achieve the detection on the microorganisms in a vaginal secretion sample, a cervical exfoliated cell sample, a skin sample and a sputum sample, the detection result can be visualized, and the microorganism variety can be directly and reasonably judged.

The invention belongs to the technical fields of bioelectrochemistry and biochip sensors and relates to an electrochemical biochip sensor array for mycobacterium tuberculosis 16SrDNA and for detecting mycobacterium tuberculosis and a preparing method thereof. Starting with molecular level detection of the mycobacterium tuberculosis 16SrDNA, Fe3O4@SiO2 composite nanometer particles and a nanometer Au structure material are adopted as mediators, a novel and functionalized nanometer electrochemical biochip superparamagnetism nano-detection microsphere sensor interface is designed, the mycobacterium tuberculosis is subjected to double enrichment extraction and purification detection, and the electrochemical biochip sensor array for rapidly detecting the mycobacterium tuberculosis is constructed. A clinical sample containing the mycobacterium tuberculosis is directly added into a magnetic reaction micropore. A mycobacterium tuberculosis 16SrDNA specific sequence can be subjected to double enrichment and biological stabilization through utilizing a capture probe labeled by the Fe3O4@SiO2 composite nanometer particles and a nanometer Au labeled detecting probe respectively. A high mycobacterium tuberculosis target molecule separating efficiency and high detection sensitivity are expected to be achieved by utilization of nano-detection microsphere superparamagnetism. In addition, target molecule "on-chip" separation is expected to be achieved through characteristics of the electrochemical biochip sensor array without the need of extra separating steps, thus largely simplifying operation procedures and shortening the detection time.

The invention discloses a nucleic acid probe combination, a kit and a method for detecting common pathogens in genital tracts. The nucleic acid probe combination comprises one or more groups of multiplex specific primer combinations and / or one or more probe combinations sequentially fixed on the surface of a membrane chip. The primer combination comprises primers of common pathogens in genital tracts and primers of endogenous control GAPDH genes. The probe combination comprises a specific probe for common pathogens in genital tracts, a positive dot hybridization probe and an endogenous control GAPDH gene probe. According to the invention, a multiplex PCR and reverse dot hybridization combined technology is adopted to solve problems of low assay accuracy of conventional primers and probes on pathogens in genital tracts and difficulty in realizing parallel assay of single reaction systems for more than seven pathogens in genital tracts. The nucleic acid probe combination of the present invention overcomes defects of easiness in false positive result caused by cross contamination and low accuracy in multiplex reaction systems in the prior art, and has the advantages of quick detection, convenience in operation, high accuracy and sensitivity, good specificity and visible detection results.

The invention discloses the establishment of a visual rapid detection method of a Bartonella henselae genome DNA, and belongs to the technical field of biology. A pair of high-specificity and strong-sensitivity RPA primers, namely an upstream primer (210)2 and a downstream primer (210)3, are screened, and an RPA (recombinase polymerase amplification) detection system of the Bh genome DNA is established. Compared with the conventional PCR method, the RPA-LFA method has the advantages of low requirements on instrument equipment (only one thermostat water bath is needed), high detection speed (about 40min), visualization of a detection result (the detection result can be directly observed by naked eyes) and the like, so that because of the advantages, the RPA-LFA method provided by the invention can be applied to rapid detection and diagnosis of cat Bh infection in a pet hospital.

The invention relates to a novel detection kit for transgenic aspergillus oryzae and a detection method thereof, and relates to the technical field of transgenic microbiological detection. By designing a labeled primer and a probe for specific sequences of transgenic aspergillus oryzae and combining PCR amplification with nucleic acid test strip detection technology, the invention realizes accurate, high-efficient and rapid detection of transgenic aspergillus oryzae. The method has strong specificity, high sensitivity and good stability, and plays an important role in improving accuracy and shortening detection time for transgenic aspergillus oryzae detection. The detection kit is convenient and rapid for use, simple in principle and operation, and applicable to rapid transgenic aspergillus oryzae detection.

The invention provides a primer for detecting metapneumovirus. Nucleic acid detection of the metapneumovirus is carried out by specifically amplifying an N gene of C-type metapneumovirus. The invention further provides an metapneumovirus detection kit, visualization of a detection result is achieved through RTPCR amplification, nanogold tracing and double signal amplification, the kit is used for metapneumovirus detection, the detection lower limit can be greatly reduced, and meanwhile the specificity, sensitivity, accuracy and repeatability of virus detection are effectively improved. When the kit is used for detecting metapneumovirus, the pretreatment process of a sample is simple, consumed time is short, and a large number of samples can be detected simultaneously.

The invention discloses a PCR (Polymerase Chain Reaction) high-resolution melting detection kit for 15 animal-derived components and a detection method. According to the technical scheme, the invention discloses a PCR (Polymerase Chain Reaction) high-resolution melting detection kit for 15 animal-derived components and a detection method. Through primer optimization screening, three pairs of universal primers are adopted for combined detection, the melting temperature Tm of species is used as a characteristic value to establish a standard library, and 15 animal-derived components of cattle, buffalo, yak, goat, sheep, rabbit, horse, donkey, pig, dog, cat, mouse, fox, mink and raccoon dog in meat and meat products can be rapidly, simply and conveniently detected. The method is simple, convenient and rapid, agarose electrophoresis is not needed, cross contamination is avoided, and corresponding specific primers do not need to be designed for each species; by referring to PCA and LDA analysis methods in statistics, the detection result is more visual; compared with various traditional PCR technologies, the method has the advantages that the problem of competition among primers is solved, the detection efficiency is greatly improved, and the reaction cost is reduced.

The invention discloses an acetone gas detector based on charge transfer and a detection method thereof, wherein the detector comprises a gas input channel, a response cavity, ultraviolet diodes, a MoTe2 sensitive layer, micro metal electrodes, gold wires, a display screen, a current sampling chip and a gas output channel. The MoTe2 sensitive layer generates electron hole pairs under the excitation of the ultraviolet diodes; when the organic gas acetone adsorbs, the electron hole pairs are separated, and a large amount of electrons are transferred to acetone molecules, causing the current to rise sharply; and when other organic gases adsorb, only a small amount of electron holes are transferred to the organic gas molecules, so that the resulting change in the current can be substantially negligible. According to the acetone gas detector based on charge transfer and the detection method thereof, the specific detection of acetone in the mixed organic gas is achieved with the high sensitivity and specific selection for acetone.

The invention discloses a primer and a detection method for detecting Actinidia chlorotic ringspot-associated virus (AcCRaV) based on RT-LAMP-LFD; the primers include inner primers: FIP and BIP and outer primers: F3 and B3. The detection method comprises the following steps: S1, extracting the RNA of the kiwi fruit sample to be detected; S2, reverse-transcribing the extracted kiwi fruit sample RNA to be detected into cDNA; S3, using the cDNA of the kiwi fruit sample to be detected as a template to perform isothermal amplification; S4, utilizing The LFD test strip detects the isothermal amplified product obtained in step S3 to determine whether the kiwifruit sample to be tested contains kiwifruit chlorotic ring spot-associated virus. The invention designs LAMP primers according to the nucleotide sequence of the coat protein of kiwifruit chlorotic ring spot-associated virus, realizes the rapid detection of kiwifruit chlorotic ring spot-related virus, and can quickly identify whether the virus is contained in kiwifruit plants or tissue culture seedlings , so as to achieve the purpose of detection, monitoring, early warning and take targeted measures to reduce the losses caused by diseases.

The invention provides a method for detecting hydrogen peroxide based on a bimetallic Co / Mn-MOFs enzyme-like property colorimetric method and belongs to the technical field of environmental monitoring. The method comprises: synthesizing two-dimensional bimetallic Co / Mn-MOFs through a one-step hydrothermal method. Through the synergistic catalysis effects of two metals in the material and a large specific surface area and accessible active sites of the two-dimensional structure, the Co / Mn-MOFs have good enzyme-like activity. When H2O2 exits, the material can promote decomposition of H2O2 so that a hydroxyl radical .OH is produced and the .OH oxidizes a chromogenic substrate TMB into oxTMB and thus the reaction system is changed from colorless to blue. The colorimetric sensing method has a H2O2 linear detection range of 1 to 100 micromoles per liter and a detection limit of 0.85 micromoles per liter. The method has the characteristics of simpleness, visuality, low cost and high sensitivity and provides a novel idea for use of the material in environmental monitoring and biological analysis.