15results about How to "Achieving Specific Detection" patented technology

The invention belongs to the technical field of colorimetric chemical sensors, and particularly relates to a preparation and application method of a smartphone-assisted surface molecular imprinting colorimetric sensor for detecting norfloxacin, magnetic Fe3O4 (at) KH540 nanoparticles are taken as a matrix, magnetic norfloxacin surface molecular imprinting nano-enzyme MMIPs is prepared by adopting a surface molecular imprinting technology, then the MMIPs, H2O2 and 3, 3 ', 5, 6, 7-tetramethyl-3, 5, 6-tetramethyl-3, 5, 6-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3 The colorimetric sensor for detecting norfloxacin is constructed by carrying out a chromogenic reaction on 3, 3 ', 5, 5'-tetramethyl benzidine TMB and cooperating with a smartphone. The sensor has specific recognition selectivity and binding affinity for NOR, is simple to operate, high in detection speed and low in cost, does not need expensive instruments, and can be suitable for field analysis.

The invention relates to the technical field of non-replicative adenovirus vectors, in particular to a primer combination and a method for detecting residual DNA fragments in an E1 region of a host cell of a non-replicative adenovirus vector. The primer combination comprises an E1A primer pair and an E1B primer pair, the E1A primer pair comprises a first primer as shown in SEQ ID NO. 1 and a second primer as shown in SEQ ID NO. 2, and the E1B primer pair comprises a third primer as shown in SEQ ID NO. 3 and a fourth primer as shown in SEQ ID NO. 4. The primer combination can be used for carrying out specific detection on E1 residual fragments in HEK293 cell host DNA (Deoxyribose Nucleic Acid); the detection method has the advantages of high efficiency, good effect, high specificity, good sensitivity and good repeatability.

The invention discloses a preparation method and application of a colorimetric / SERS (Surface Enhanced Raman Scattering) dual-mode biosensor, and belongs to the technical field of food safety detection. The preparation method of the sensor comprises the following steps: carrying out amino modification on MIL-101 nanoparticles to obtain aminated MIL-101; taking the aminated MIL-101 and Au-NRs as raw materials, and carrying out reaction to prepare an MIL-101 / Au NRs nano composite material; and mixing the MIL-101 / Au NRs nano composite material with a sodium acetate buffer solution, a TMB (Tetramethylbenzidine) solution and an H2O2 solution, and reacting at the temperature of 40-70 DEG C for 0.2-2 hours to obtain the sensor. According to the colorimetric / SERS dual-mode biosensor constructed by the invention, various analysis and detection of CAP in the same sensor are realized, so that the field requirement is met to the greatest extent, and the complex and variable conditions in the detection field are dealt with.

The present invention discloses a method for specific detection of samples using terahertz band polyaniline metamaterials. A terahertz band polyaniline metamaterial is prepared, which includes polyaniline, a metal anti-transmission layer, and a silicon substrate from top to bottom; a sample point to be tested is set and a terahertz time domain signal is collected; a sample solution is prepared; the sample solution is dripped onto the sample point to be tested, and the terahertz time domain signal is collected after drying; the terahertz band polyaniline metamaterial is rinsed, and the terahertz time domain signal is collected after drying; the terahertz time domain signal of air is collected; the transmittance change is obtained according to the terahertz time domain signal, and the detection sample is judged according to the transmittance change. The present invention utilizes the interaction between polyaniline and the sample to change the terahertz transmission spectrum of the terahertz band polyaniline metamaterial, thereby amplifying the sample signal. This method can achieve specific detection of the sample to be tested and meet the growing demand for accurate detection.

This invention relates to a method for preparing a bimodal hydrogel detection reagent for carbofuran residues in cucumbers, belonging to the field of food safety testing. The bimodal hydrogel detection reagent is a hydrogel-like detection reagent synthesized by adding a copper-doped metal-organic framework to a sodium alginate-calcium carbonate hydrogel system. The bimodal hydrogel detection reagent is a three-dimensional network structure material. The detection limit of the bimodal hydrogel detection reagent for carbofuran in cucumbers is 17.59 μM in the colorimetric mode (0-250 μM) and 5.98 μM in the fluorescence mode (0-180 μM), achieving bimodal detection. This not only enables real-time on-site monitoring of carbofuran residues in cucumbers but also ensures the accuracy of the detection results through cross-validation between the two modes; the detection time is within 1.5 hours, and it exhibits good specificity for carbofuran residues in cucumbers.

The application belongs to the field of optical fiber sensing, and particularly relates to a high-sensitivity optical fiber SPR traditional Chinese medicine active ingredient sensor and a manufacturing and using method thereof; the sensor comprises a core offset light injection area, a mode modulation and compression area and a coarse core light receiving area which are sequentially fused, the mode modulation and compression area is in three modes of a groove type of graded-index fiber, a taper spiral type of core offset fiber and a flat bottom partial sphere type of core offset fiber, gold films and target proteins are arranged on the mode modulation and compression area from inside to outside, an optical signal is injected into the mode compression and modulation area from the core offset light injection area, after modulation, the optical signal mode is concentrated and the light incidence angle is effectively modulated, the sensor sensitivity is greatly improved, transmission light contacts the gold films to generate an SPR effect, the target proteins are specifically combined with traditional Chinese medicine active ingredients, an SPR resonance valley moves, and specific detection of traditional Chinese medicine active ingredients is realized. The application solves the problems of low sensitivity and difficulty in specific detection of the current optical fiber SPR traditional Chinese medicine active ingredient sensor.

The application belongs to the technical field of gene detection, and particularly relates to specific primers, a Taqman probe and application of a bacillus velezensis HMB26553 strain. The specific primers and the probe sequence of the bacillus velezensis HMB26553 strain are respectively SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The primers can amplify a single unique target fragment from the bacillus velezensis HMB26553 strain, and realize specific detection of the bacillus velezensis HMB26553 strain. The primers have the characteristics of high specificity, high detection efficiency, low false positive rate, long preservation time and the like. By using the primers and through real-time quantitative PCR technology, the population quantity of the bacillus velezensis HMB26553 strain in the rhizosphere of cotton can be quickly quantified, and the primers have the advantages of high throughput, rapidness and accuracy.

The present invention belongs to the field of modern agricultural technology, and specifically relates to a kit for detecting Phytophthora palmitoides, a durian root rot disease, and its application. The present invention designs primers and crRNA for the specific sequence of Phytophthora palmitoides, and develops a rapid visualization detection reagent and kit for Phytophthora palmitoides, a durian root rot disease. The reagent or kit can complete the detection of Phytophthora palmitoides, a durian root rot disease, within 30 minutes, has good specificity and no cross-reaction, and the sensitivity reaches 10 fg / μL. And there is no need to open the cover after the RPA amplification is completed to add the CRISPR / Cas12a reaction system, avoiding aerosol contamination caused by opening the cover. The present invention does not require the use of large-scale instruments and equipment, has the characteristics of short time consumption, simple operation, high sensitivity and specificity, and is suitable for rapid visualization detection of clinical samples and on-site.

The invention provides a dual-signal molecularly imprinted electrochemical sensor based on Cu / Fe-MOF (Metal Organic Framework) and AuNPs (AuNPs) and a preparation method of the dual-signal molecularly imprinted electrochemical sensor. Carbon cloth is used as a substrate, Cu / Fe-MOF grows in situ, an AuNPs modified electrode is electrically deposited, and a double-signal system is constructed; and preparing the molecularly imprinted polymer film layer by using semicarbazide as a template through electro-polymerization. According to the sensor, high-sensitivity detection of semicarbazide is achieved through double-signal change, the linear range is 0.005-100 nM, and the detection limit is 0.0039 nM. According to the invention, a large specific surface area material and a molecular imprinting technology are combined, so that the detection sensitivity and specificity are improved; the dual-signal mode enhances the detection stability and accuracy; the preparation method is simple, low in cost, suitable for quantitative analysis of trace substances and wide in application prospect.

The invention belongs to the technical field of biological analysis, and particularly relates to a one-pot method, Cas12a cis-cleavage, in-vitro transcription, a fluorescent RNA aptamer and application of the fluorescent RNA aptamer in microRNA detection in a material sample. The invention provides a method for sensitively and specifically detecting microRNA by connecting, identifying and triggering RPA (recombinase polymerase amplification) amplification and Cas12a cis-cleavage to realize large-scale amplification of a double-stranded DNA (deoxyribonucleic acid) amplicon and transcription of a functional fluorescent RNA aptamer, and combining a mechanism strategy of T7 transcription signal amplification and fluorescent aptamer signal amplification. The invention also provides a method for realizing rapid detection of miRNA-21 by optimizing the reaction process through a one-pot method. By utilizing the high sensitivity, high specificity and label-free characteristics of a detection platform for connecting and recognizing RPA amplification and Cas12a cis-cleavage biosensor through the one-pot method, the miRNA-21 concentration in cancer cells and patient serum samples can be quickly and effectively detected.

The invention discloses a method for detecting gelatin in sea cucumber based on a fluorescent PCR (Polymerase Chain Reaction) technology. The method comprises the following steps: determining a target and designing a primer and a probe; determining a target gene: selecting a mitochondrial gene aiming at the DNA which is easy to degrade after processing, and designing a primer and a probe for amplifying a short fragment; obtaining a specific sequence: obtaining target gene sequences of the target sea cucumber and common adulterated species from an NCBI gene database, and finding a unique specific sequence region of the sea cucumber through comparison; synthesizing a primer and a probe: designing and synthesizing the primer and the TaqMan probe on the basis of the specific region; preparing a reference substance; sample pretreatment and DNA extraction; preparing a fluorescent PCR reaction system and adding a sample; running a fluorescent PCR amplification program: placing the reaction tube in a real-time fluorescent quantitative PCR instrument; result analysis and interpretation: quality control inspection and sample result interpretation. According to the invention, a primer and a probe which are only complementarily combined with sea cucumber DNA are designed, and a fluorescence signal is generated in PCR amplification, so that specific detection can be realized.

This invention belongs to the field of biodetection technology and provides a rapid nucleic acid detection method for Anopheles hucanense. This method involves designing specific primers and probes based on the Anopheles hucanense sequence, validating the results through enzymatic recombination isothermal amplification experiments, and screening specific primer pairs for rapid detection of Anopheles hucanense. This invention can efficiently and rapidly amplify specific fragments of trace genomes within a short time, enabling rapid detection and differentiation of Anopheles hucanense. Furthermore, this method exhibits high sensitivity and specificity, requires no large-scale equipment, and is suitable for rapid on-site detection, which is of great significance for the epidemic control and treatment of mosquito-borne diseases such as malaria.

The application discloses an immune response hydrogel, an immune sensing detection device and a preparation method. The preparation steps of the immune response hydrogel of the application include vinyl modification of an antigen protein, preparation and antibody coupling of gold nanoparticles, polymerization of the vinyl modified antigen protein and immune cross-linking of the antibody coupled gold nanoparticles, and finally the immune response hydrogel with the immune response function is obtained. The wireless immune detection device includes an immune response hydrogel resonance sensor, a readout coil and the like. The device can realize wireless readout of multiple sensing signals through extension of parallel intermediate coils, and meanwhile, the immune hydrogel resonance sensor and the intermediate coil are assembled in an article such as a mask, so that wearable detection of antigen proteins can be realized. The technology provides a rapid, sensitive, accurate and instant detection platform for applications such as virus antigen protein monitoring and wearable immune biochemical analysis.

The invention provides a primer pair and a kit for detecting pear fire blight bacteria and application of the primer pair and the kit, and belongs to the technical field of biological agriculture detection. The invention provides a specific DNA fragment for detecting pear fire blight bacteria. The nucleotide sequence of the specific DNA fragment is as shown in SEQ ID NO: 1. The invention also provides a primer pair for amplifying the specific DNA fragment. The primer pair comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO: 2 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO: 3. The primer pair disclosed by the invention can be used for carrying out conventional PCR detection and / or real-time fluorescent quantitative PCR detection on pear fire blight bacteria, has the characteristic of high specificity, and can realize specific detection without being combined with a fluorescent probe.