7results about How to "Achieving Specific Detection" patented technology

This invention relates to a method for preparing a bimodal hydrogel detection reagent for carbofuran residues in cucumbers, belonging to the field of food safety testing. The bimodal hydrogel detection reagent is a hydrogel-like detection reagent synthesized by adding a copper-doped metal-organic framework to a sodium alginate-calcium carbonate hydrogel system. The bimodal hydrogel detection reagent is a three-dimensional network structure material. The detection limit of the bimodal hydrogel detection reagent for carbofuran in cucumbers is 17.59 μM in the colorimetric mode (0-250 μM) and 5.98 μM in the fluorescence mode (0-180 μM), achieving bimodal detection. This not only enables real-time on-site monitoring of carbofuran residues in cucumbers but also ensures the accuracy of the detection results through cross-validation between the two modes; the detection time is within 1.5 hours, and it exhibits good specificity for carbofuran residues in cucumbers.

The application belongs to the field of optical fiber sensing, and particularly relates to a high-sensitivity optical fiber SPR traditional Chinese medicine active ingredient sensor and a manufacturing and using method thereof; the sensor comprises a core offset light injection area, a mode modulation and compression area and a coarse core light receiving area which are sequentially fused, the mode modulation and compression area is in three modes of a groove type of graded-index fiber, a taper spiral type of core offset fiber and a flat bottom partial sphere type of core offset fiber, gold films and target proteins are arranged on the mode modulation and compression area from inside to outside, an optical signal is injected into the mode compression and modulation area from the core offset light injection area, after modulation, the optical signal mode is concentrated and the light incidence angle is effectively modulated, the sensor sensitivity is greatly improved, transmission light contacts the gold films to generate an SPR effect, the target proteins are specifically combined with traditional Chinese medicine active ingredients, an SPR resonance valley moves, and specific detection of traditional Chinese medicine active ingredients is realized. The application solves the problems of low sensitivity and difficulty in specific detection of the current optical fiber SPR traditional Chinese medicine active ingredient sensor.

The application belongs to the technical field of gene detection, and particularly relates to specific primers, a Taqman probe and application of a bacillus velezensis HMB26553 strain. The specific primers and the probe sequence of the bacillus velezensis HMB26553 strain are respectively SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The primers can amplify a single unique target fragment from the bacillus velezensis HMB26553 strain, and realize specific detection of the bacillus velezensis HMB26553 strain. The primers have the characteristics of high specificity, high detection efficiency, low false positive rate, long preservation time and the like. By using the primers and through real-time quantitative PCR technology, the population quantity of the bacillus velezensis HMB26553 strain in the rhizosphere of cotton can be quickly quantified, and the primers have the advantages of high throughput, rapidness and accuracy.

The invention discloses a method for detecting gelatin in sea cucumber based on a fluorescent PCR (Polymerase Chain Reaction) technology. The method comprises the following steps: determining a target and designing a primer and a probe; determining a target gene: selecting a mitochondrial gene aiming at the DNA which is easy to degrade after processing, and designing a primer and a probe for amplifying a short fragment; obtaining a specific sequence: obtaining target gene sequences of the target sea cucumber and common adulterated species from an NCBI gene database, and finding a unique specific sequence region of the sea cucumber through comparison; synthesizing a primer and a probe: designing and synthesizing the primer and the TaqMan probe on the basis of the specific region; preparing a reference substance; sample pretreatment and DNA extraction; preparing a fluorescent PCR reaction system and adding a sample; running a fluorescent PCR amplification program: placing the reaction tube in a real-time fluorescent quantitative PCR instrument; result analysis and interpretation: quality control inspection and sample result interpretation. According to the invention, a primer and a probe which are only complementarily combined with sea cucumber DNA are designed, and a fluorescence signal is generated in PCR amplification, so that specific detection can be realized.

This invention belongs to the field of biodetection technology and provides a rapid nucleic acid detection method for Anopheles hucanense. This method involves designing specific primers and probes based on the Anopheles hucanense sequence, validating the results through enzymatic recombination isothermal amplification experiments, and screening specific primer pairs for rapid detection of Anopheles hucanense. This invention can efficiently and rapidly amplify specific fragments of trace genomes within a short time, enabling rapid detection and differentiation of Anopheles hucanense. Furthermore, this method exhibits high sensitivity and specificity, requires no large-scale equipment, and is suitable for rapid on-site detection, which is of great significance for the epidemic control and treatment of mosquito-borne diseases such as malaria.

The application discloses an immune response hydrogel, an immune sensing detection device and a preparation method. The preparation steps of the immune response hydrogel of the application include vinyl modification of an antigen protein, preparation and antibody coupling of gold nanoparticles, polymerization of the vinyl modified antigen protein and immune cross-linking of the antibody coupled gold nanoparticles, and finally the immune response hydrogel with the immune response function is obtained. The wireless immune detection device includes an immune response hydrogel resonance sensor, a readout coil and the like. The device can realize wireless readout of multiple sensing signals through extension of parallel intermediate coils, and meanwhile, the immune hydrogel resonance sensor and the intermediate coil are assembled in an article such as a mask, so that wearable detection of antigen proteins can be realized. The technology provides a rapid, sensitive, accurate and instant detection platform for applications such as virus antigen protein monitoring and wearable immune biochemical analysis.