240 results about "Norfloxacin" patented technology

A conjugate of norfloxacin is prepared from norfloxacin semi-antigen and the bovine serum albumin (or egg albumin) as the carrier able to generate immunogenicity through coupling. It can be used for preparing the reagent kit for the enzyme-linked immunoassay of norfloxacin.

The invention discloses a solid phase extraction and HPLC-fluorescence detection method for fluoroquinolones antibiotics. The method comprises the following steps of passing a pretreated water sample through an activated solid phase extraction column; eluting the solid phase extraction column with a Na2EDTA aqueous solution; then vacuumizing; and finally, eluting the water sample for three times by a concentrated ammonia methanol solution with a concentrated ammonia volume percent concentration of 6 percent by 0.5-2mL each time. An obtained eluent is blow-dried to 0.1-0.4mL in air flow, water is added to reach a constant column of 1.0mL so as to obtain a test sample, and then HPLC-fluorescence detection is carried out. By adopting the solid phase extraction-HPLC-fluorescence detection technology and utilizing a conventional analytical instrument, the method can simultaneously measure the contents of four typical fluoroquinolones antibiotics of ofloxacin, norfloxacin, ciprofloxacin and enrofloxacin in the water sample. The method has simple operation and low cost and can be applied to the measurement of the ultratrace of four typical fluoroquinolones antibiotics in waste water and surface water.

Belonging to the technical field of biochar preparation, the invention discloses preparation of Fe-Zn and phosphate modified sludge biochar and application of the same in removal of fluoroquinolones antibiotics from water. The preparation method includes: dewatering and drying the sludge from a secondary sedimentation tank of a municipal sewage treatment plant, then performing pyrolysis by a hightemperature tubular furnace, then utilizing Fe-Zn and phosphate for modification, and finally conducting two calcining to obtain Fe/Zn+H3PO4-SBC. The Fe/Zn+H3PO4-SBC prepared by the invention has adsorption capacity on fluoroquinolones antibiotics ciprofloxacin, norfloxacin and ofloxacin up to 25.43-88.73mg/g. The method provided by the invention adopts municipal sludge as the raw material, reduces the production cost, realizes the resource utilization of municipal sludge and efficient removal of antibiotics from water, also has the advantages of simple operation, low cost and the like, and isa potential removal method for fluoroquinolones antibiotics in water.

The invention discloses a synthesis method of a molecularly imprinted composite membrane capable of enriching and separating norfloxacin, and belongs to the technical field of a new material. The molecularly imprinted composite membrane is synthesized mainly through three-step reaction. The synthesis method comprises the following steps: firstly, synthesizing titanium dioxide nanoparticles; secondly, on the substrate of the titanium dioxide, synthesizing a norfloxacin molecularly imprinted polymer by taking the norfloxacin as a template molecule, taking dopamine as a functional monomer and a crosslinking agent and combining with a dopamine bionic principle; finally, performing suction filtration on the molecularly imprinted polymer on the membrane by taking a regenerated cellulose membrane as a matrix material and through a vacuum suction filtration method for detecting the residual norfloxacin in water. The preparation method provided by the invention has the characteristics of simplicity and convenience in operation, easiness in implementation, high yield, middle reaction condition and the like, and is expected to be applied to industrial production. In addition, static adsorption and selective permeation experimental results indicate that the prepared molecularly imprinted composite membrane has high separation property and high selectivity.

The invention belongs to the field of medical products, particularly relates to an antibacterial hydrogel wound coating film and a preparation method thereof, and also relates to a wound paste prepared by using the coating film. The antibacterial hydrogel wound coating film comprises the following components by mass percent: 0.1-5% of norfloxacin silver, 5-30% of polymer, 0-10% of plasticizer and the balance of water. The antibacterial hydrogel wound coating film disclosed by the invention can be directly used for covering a wound to treat various wounds caused by burns, scalds, trauma, surgical operations and the like, and is capable of absorbing body fluid secreted by the wounds and keeping the wounds in a clean and moist state. Meanwhile, the coating film also has good antibacterial effect, so that invasion of bacteria is cut off; infection is prevented; union of the wounds can be facilitated.

The invention relates to a method for detecting residual quantity of norfloxacin antibiotic in milk. The method utilizes an ultra-performance liquid chromatography (UPLC)-electrospray tandem triple quadrupole mass spectrometer to determine the residual quantity of norfloxacin antibiotic. A sample is extracted by Na2 EDTA-Mellvaine buffer solution (pH 4.0), subjected to protein removal by trichloroacetic acid, purified and concentrated by a PEP solid phase extraction columella, separated by adopting a chromatographic column with column temperature of 30 DEG C, subjected to gradient elution by adopting aqueous solution (v/v) containing 0.1% formic acid and acetonitrile as a flowing phase, and then quantitated by adopting a multiple reaction monitoring manner. The instrument detection limit is 1.0-2.0 mug/Kg; and within the linear range of 1-100 mug/Kg, the correlation coefficient r can reach more than 0.999, and the coefficient of recovery is 80%-105% (adding level of 10, 50 and 100 mug/Kg). The method has the advantages of rapidness, accuracy, high sensitivity and wide application scope.

An Eleutheronema rhadinum fry live transport method includes adding tricaine methanesulfonate, YINGJINING for fish, glucose and norfloxacin in transport water body. When transport time is less than 2 hr, inflated water box is adopted, and addition amounts of tricaine methanesulfonate, YINGJINING for fish, glucose and norfloxacin are 15 ppm, 30 ppm, 10 ppm and 2 ppm, respectively. When transport time is longer than 2 hr, oxygenated fry bag is adopted, and addition amounts of tricaine methanesulfonate, YINGJINING for fish, glucose and norfloxacin are 10 ppm, 20 ppm, 50 ppm and 5 ppm, respectively. The invention can reduce stress damage of fish body, and improve transport survival rate.

The invention relates to the technical field of environment detection and is suitable for measuring erythrocin, chloramphenicol, sulfamethoxazole, sulfadiazine, roxithromycin, cefotiam, pyridazol, norfloxacin, ofloxacin, tetracycline and doxycycline in sewage, surface water and bottom mud. After being sampled, a water sample and a mud sample are pretreated and detected through ultra-high performance liquid chromatography-triple quadrupole mass spectrometry, and qualitative diagnosis is conducted through the characteristic ion pair (m/z) of a target compound, a standard curve is drawn accordingto the response value and the corresponding concentration, and the external standard method is used in quantification, so that the contents of various antibiotics in the sample are obtained. The method is accurate, sensitive and simple, and is suitable for measuring the contents of 11 typical antibiotics in the sewage, the surface water and the mud at the same time.

The invention relates to a method for simultaneously detecting 15 antibiotics in aquaculture sediment by combining microwave extraction-solid phase extraction pretreatment with a liquid chromatography-mass spectrometry (LC-MS) technology. The method comprises the following steps: step (1) pretreating sediment samples, adding internal standard substances sulfamethoxazole-13C6, norfloxacin-D5, penicillin G potassium salt-D5, tetracycline-D6, olaquindox-D4, amoxicillin-13C6 and trimethoprim-D3 indicating a recovery rate, adding an acetonitrile-phosphate buffer solution for microwave extraction ofextraction liquid; step (2) performing solid phase extraction, enrichment and purification on target antibiotics; and step (3) quantitatively detecting 15 antibiotics of seven types in sediment witha liquid chromatograph/mass spectrometer. The method can simultaneously detect residues of 15 antibiotics containing 8 new antibiotics in the aquaculture sediment at a time by optimizing the internalstandard substances, screening appropriate pretreatment method and optimizing detection conditions, and has the advantages of high recovery rate, high sensitivity, high stability, low detection limit,more true and reliable detection results and the like.

The invention discloses a rabbit monoclonal antibody based ciprofloxacin residue analysis enzyme-linked immune adsorption kit. In the ciprofloxacin detection, the IC50 value of the kit is 1.41ng/mL, the linear fitting equation of an inhibition ratio curve is y = 0.0239x - 1.3159, the detection range is between 0.19 and 10ng/mL, and the lowest detection limit is 0.095ng/mL. The ciprofloxacin antibody in the kit has higher specificity, and has lower crossing-over rate with other quinolone micromolecules; and the cross reaction rates between the ciprofloxacin antibody and enrofloxacin, ofloxacin, norfloxacin, fleroxacin or pefloxacin are 28.8 percent, 13.1 percent, 11 percent, 22.6 percent and 20.4 percent respectively. The ciprofloxacin antibody has no cross reaction with other antibiotics and sulfanilamide medicaments. The kit is suitable for detecting ciprofloxacin residue in samples of milk, urine and the like. The kit can detect samples on a large scale at the same time, is convenient and quick, has quite important realistic significance for on-site supervision and trace analysis, meanwhile greatly reduces the detection cost, and has potential economic value.

The invention discloses a berberine azole compound shown in general formulas such as I-IV, and a pharmaceutically acceptable salt thereof, and also discloses a preparation method of the compound, which comprises the following steps that: a compound VI and an azole compound HIm or 1H-2-mercaptobenzimidazole are reacted to obtain an intermediate VII or VIII, and then the obtained intermediate is reacted with a compound V to obtain the berberine azole compound shown in general formulas such as I or II; and the compound V and a compound IX or XI are reacted to obtain an intermediate X or XII, and then the obtained intermediate is reacted with the azole compound Him to obtain the berberine azole compound shown in general formulas such as III or IV. The berberine azole compound has a certain inhibitory activity on Gram-positive bacteria, Gram-negative bacteria and fungi, the anti-bacterial activity of part of the compounds is equal to or even stronger than that of chloramphenicol or norfloxacin, the antifungal activity of part of the compounds is equal to or even stronger than that of fluconazole, and the berberine azole compound can be used for preparing anti-microbial drugs.

The invention relates to a polymer material for adsorbing antibiotics, a preparation method and an application thereof. The polymer material is a polyaniline derivative polymer material obtained by reacting certain amount of acid solutions of aniline and ortho-aminophenol under the action of an initiator to generate solid precipitates, and performing separation, washing, vacuum drying, and grinding of the precipitates. The polyaniline derivative polymer material of the invention is used for adsorbing antibiotic pollutants such as tetracycline, terramycin, aureomycin, norfloxacin and the like, and has better adsorptive property than conventional adsorption materials. The polyaniline derivative has a maximum adsorption capacity of 127.14 mg/g, and the preparation method of the invention is simple in steps, low in cost, and convenient to operate. The polymer material and the preparation method of the invention have wide application prospects in the field of antibiotic treatment for sewage.

The invention discloses a method for preparing norfloxacin tablets. The method comprises the following steps of: sieving norfloxacin with a sieve of 40 to 100 meshes, sieving a disintegrating agent with a sieve of 60 to 100 meshes, sieving a lubricating agent and a flow aid with a sieve of 80 to 120 meshes, mixing the norfloxacin with a large part of disintegrating agent uniformly, mixing less than 2 percent of disintegrating agent, the lubricating agent and the flow aid uniformly, adding into the mixed powder, mixing uniformly, tabletting, and thus obtaining the norfloxacin tablets. The norfloxacin tablets comprise the following raw materials in percentage by weight: 20 to 80 percent of norfloxacin, 5 to 79 percent of disintegrating agent, and 1 to 8 percent of lubricating agent and flow aid. 5 to 70 percent of filling agent can also be added. The mixed materials obtain high flowability, excellent compression property and adhesive property, high attachment and low sensitivity to the lubricating agent through a large quantity of tests, advantage complementation of auxiliary materials, proper formula proportioning, reasonable granularity distribution and accurate powder mixing sequence. The method overcomes the defects of common material non-layering, non-uniform content, high tablet height difference, tablet powder fall, slow disintegration and the like in the direct tabletting production process, and solves the problems of color change of tablet cores, low dissolubility and the like of the wet granulation process.

The invention discloses a technology for identifying packaged capsules on the basis of terahertz time-domain spectral imaging technologies. Medicines mainly include capsule medicines such as norfloxacin, amoxicillin and cefradine. The technology approximately includes steps of (1), preparing standard samples of a plurality of groups of capsule medicines and eliminating different types of environmental noise and influence of different types of environments to obtain terahertz absorption spectra of the various samples; (2), selecting optical parameters of the various standard samples in terahertz wave band effective intervals and creating three-dimensional characteristic spectrum databases of the optical parameters; (3), arranging effective optical parameters of the to-be-detected medicines in the previously created three-dimensional characteristic spectrum databases and comparing spectrogram absorption peaks of the effective optical parameters so as to classify and identify the medicines and judge whether the to-be-detected medicines are pure or not. The technology has the advantages that medicine components in the capsules can be efficiently and accurately identified by the aid of the technology, and accordingly the technology has a broad application prospect in the fields of medicine analysis, medicine detection and the like.

The present invention discloses a quinolone drug chemiluminescence enzyme-linked immunodetection kit, which comprises a kit body, an enzyme label plate placed inside the kit body, and reagents placed inside the kit body, and is characterized in that every hole of the enzyme label plate is coated with coating antigen, the coating antigen is a norfloxacin derivative and carrier protein conjugate, and the reagents comprise quinolone drug monoclonal antibody, horseradish peroxidase-labeled goat anti-mouse antibody, a series of quinolone drug standard solutions, a concentrated phosphate buffer, a concentrated washing solution and a chemiluminescence solution. The quinolone drug chemiluminescence enzyme-linked immunodetection kit has characteristics of high sensitivity, simple and rapid detection, high accuracy, and more drug detection types, provides a substantially reduced operation time compared to the conventional colorimetric ELISA method, and can be used for detection of residues of the 12 quinolone drugs in animal tissues (pork, chicken, pork liver and chicken liver), aquatic products (fish and shrimp) and honey.

The compound medicine preparation for curing diarrhea and dysentery is made up by using norfloxacin and 16 Chinese medicinal materials of hericium, cyperus root, bitter organe, licorice, unripe tangerine peel, tangerine peel and others as raw material.

The invention discloses an air disinfectant, prepared from the following raw materials in parts by weight: 5-10 parts of hydrogen peroxide, 4-8 parts of chlorine dioxide, 1-3 parts of lauroyl diethanolamide, 0.5-1 part of norfloxacin, 12-16 parts of ethyl alcohol, 2-4 parts of mint oil, 4-8 parts of folium artemisia argyi extract liquor, 2-4 parts of tea tree essential oil, 1-3 parts of mint oil, 0.5-1 part of wrinkled gianthyssop herb and 35-45 parts of deionized water. The air disinfectant does not contain an aromatic preparation, and is safe, non-toxic, pungent smell-free, harmless to a body, and good in sterilizing effect.

The invention belongs to the field of animal medicine and particularly relates to a medicine for treating transport stress of animals, specifically to a micro-ecological compound preparation for preventing and treating stress diarrhoea of a goat. The micro-ecological compound preparation comprises a composition and Lactobacillus Sporogenes, wherein the composition comprises astragalus polysaccharide, multiplex vitamin of electrolyte, medical active carbon and wheat bran/rice bran, and each g of the composition is mixed with 10<9> to 10<11> CFU of animal-born Lactobacillus Sporogenes. The effective rate of the micro-ecological compound preparation in treatment and prevention is 100%. Compared with a bacitracin zinc group and a norfloxacin group, the micro-ecological compound preparation provided by the invention has significantly improved effects.

The invention discloses a sulfur-doped carbon quantum dot fluorescence sensitization method for testing norfloxacin. The method employs poly(4-styrene sulfonic acid-copolymerization-maleate)sodium as a carbon source and a sulfur source, a hydrothermal method is employed for synthesizing the sulfur-doped carbon quantum dot; the sulfur-doped carbon quantum dot can enhance the fluorescence of norfloxacin, the enhanced fluorescence signal intensity and the norfloxacin concentration show a linear relationship, so that a fluorospectrophotometry for detecting the norfloxacin is established. The sulfur-doped carbon quantum dot has obvious fluorescence sensitization effect on the norfloxacin, has strong selectivity, and has the characteristics of high sensitivity and specificity for fluorescence determination for the norfloxacin.

The invention discloses preparation of a biochar-based catalyst and a method for repairing antibiotics by using the biochar-based catalyst. According to the preparation of the biochar-based catalyst and the method for repairing antibiotics by using the biochar-based catalyst, firstly, pretreated pyrite acidic mine wastewater is taken as a modifier of agricultural waste pomelo peel biochar; then, iron, copper and aluminum ions on the biochar form a high-activity biochar-based zero-valent iron, copper and aluminum ternary metal composite catalyst material under the reduction action of a pomelo peel extracting solution; a novel Fenton oxidation technical system is constructed by the high-activity biochar-based zero-valent iron, copper and aluminum ternary metal composite catalyst material andpersulfate; the novel Fenton oxidation technical system is used for single, synchronous and efficient degradation and removal of water antibiotics tetracycline and norfloxacin, within the reaction time of 60 min, the single removal rates of tetracycline and norfloxacin are as high as 98.85% and 96.52% respectively; and during synchronous removal, the removal rate can also reach 80% or above. In addition, the high-activity biochar-based Fenton catalyst has good stability. According to the method disclosed by the invention, the treatment and recycling of acid mine wastewater and agricultural waste pomelo peel are realized, and the single and synchronous degradation and removal of various antibiotics are also realized.

The invention provides a preparation method of a microbial immobilized gel sphere, and an application of the microbial immobilized gel sphere in the removal of norfloxacin and tetracycline in water. Anovel water treatment reagent is synthesized by using the good adsorption performance of calcined hydrotalcite (CLDH), the non-toxicity, the adsorption property and the good formability of sodium alginate (SA) and the degradability of microbes, and is used for removing norfloxacin, tetracycline and other antibiotics in water. The microbes capable of degrading antibiotics are immobilized in a gelsphere by a gel technology, so the endurance capability and the degradation capability of the immobilized microbes to toxic substances are improved, the microbes are prevented from being lost with outlet water, a high microbe concentration can be kept for a long term, and the interference resistance and the stability of a water treatment system are enhanced.

The invention provides a method for simultaneously detecting 15 antibiotics in an aquaculture water body by solid-phase extraction pretreatment combined with a liquid chromatography-mass spectrometrytechnology. The method comprises the following steps: (1) water sample pretreatment: adding seven internal standard substances for indicating the recovery rate, namely sulfamethoxazole-13C6, norfloxacin-D5, penicillin G potassium salt-D5, achromycin-D6, olaquindox-D4, amoxicillin-13C6 and trimethoprim-D3; (2) enriching and purifying target antibiotics by using a solid-phase extraction column; (3)detecting the contents of the 15 antibiotics in seven categories in the aquaculture water body with a liquid chromatograph/mass spectrometer by adopting an internal standard method. The method provided by the invention has the benefits that with the adoption of a single-factor experiment, the residue situations of the 15 antibiotics containing 8 new antibiotics in the aquaculture water body can besimultaneously detected at a time by preferably selecting the internal standard substances and optimizing detection conditions; the method has the advantages of high precision, high sensitivity, highstability, high selectivity, low detection limit, more true and reliable detection results and the like.

The invention relates to a medicine composition with norfloxacin and a method for preparing the medicine composition. The medicine composition comprises an effective quantity of the norfloxacin and pharmaceutical excipients. Proportions of main medicines are 50-80%; the ball forming excipients are selectively one type of microcrystalline cellulose, powdered cellulose, low-substituted hydroxypropyl cellulose and corn starch or combinations of the microcrystalline cellulose, the powdered cellulose, the low-substituted hydroxypropyl cellulose and the corn starch. The medicine composition with the norfloxacin and the method have the advantages that on the one hand, the dissolving-out speeds of oral preparations can be controlled, and on the other hand, the stability of the preparations can be improved.

The invention discloses a medical composite for treating livestock and poultry intestinal canal diseases and a preparation method and an application thereof, aiming at providing a medical composite and the preparation method and the application thereof, which can fast and effectively prevent and cure intestinal canal diseases. The composite comprises the following components in weight percent, 2%to 5% of pirquinozol antibiotics, 3% to 10% of beta-lactam antibiotics, 0.6% to 2% of beta-lactam inhibitor, 3% to 10% of aminoglycoside antibiotics and the balance auxiliary material acceptable in pharmacy. The pirquinozol antibiotics, such as norfloxacin lactate, is broad-spectrum antibiotics; the beta-lactam inhibitor has the functions of inhibiting enzyme and strengthening the effect; and thebeta-lactam antibiotics can destroy bacterial cell walls, which is favorable for aminoglycoside antibiotics to enter cells to take effect, increases the antibacterial effect and simultaneously enlarges the antibacterial spectrum. The composite of the invention can effectively cure canal diseases caused by Gram negative bacteria or anaerobe and the mixed infection of the Gram negative bacteria andthe anaerobe.

The invention discloses a microfluidic paper based sensor based on fluoroimmunoassay and utilizing a quantum dot as a marker to detect norfloxacin (NOR) in milk and belongs to the technical field of biological nanometer. A preparation method of the sensor comprises the steps: firstly, forming a hydrophilic area and a hydrophobic area on chromatographic paper through a wax spray printing technology, roasting for a certain time and performing plasma treatment on the paper; then, preparing NOR-bovine serum albumin (BSA) coating antigen and a quantum dot marked NOR antibody; then, respectively adding the coating antigen and a goat anti-mouse secondary antibody into two white paper areas in parallel up and down and adding an NOR and quantum dot marked antibody mixture into a coating layer, folding the paper and permeating the solution into a secondary antibody area; finally, utilizing immunoassay determination to finally quantitatively detect the norfloxacin in the milk through fluorescent signals. The microfluidic paper based sensor disclosed by the invention has the advantages of low cost, simpleness in operation, high flexibility, low detection limit and quickness in detecting NOR.

The present invention relates to a medicine formula for preventing and curing ascites disease in left-eyed flounder and right-eyed flounder culture. Said medicine formula includes (by wt%) 30% of norfloxacin, 30% of doxycycline and 40% of compound sinomin. Its cure rate can be up to above 85%.