27 results about "Methicillin resistant Staphylococcus" patented technology

This invention belongs to the field of biomedical functional materials technology, and relates to an antibacterial and healing-promoting photosensitizer, its preparation method, and its uses. The photosensitizer is a defective MIL-88B@MIL-88C composite material, wherein defective MIL-88B serves as the substrate, and MIL-88C grows on the surface and / or in the pores of the defective MIL-88B. This photosensitizer significantly improves the photogenerated carrier separation efficiency, reduces impedance, enhances photocurrent response, and can efficiently generate reactive oxygen species under light irradiation. Antibacterial experiments show that it has excellent bactericidal effects against Staphylococcus aureus, Escherichia coli, and methicillin-resistant Staphylococcus aureus, and is not prone to inducing drug resistance. Simultaneously, this photosensitizer has good biocompatibility, promotes cell migration, and has significant hemostatic function. Animal models show that it can effectively accelerate the healing of skin defects.

This invention discloses the application of the bacterial fission protein FtsZ inhibitor DB1 in the preparation of drugs against drug-resistant bacteria, belonging to the field of drug development technology. The inhibitor provided by this invention is compound DB1, named 3-(2,8-dibromo-10,11-dihydro-5H-dibenzo[b,f]azapheno-5-yl)-N,N-dimethylpropylamine hydrochloride, with the molecular formula C2. 19 H 24 Br2ClN2. This compound, DB1, exhibits significant inhibitory effects against various drug-resistant bacteria, particularly vancomycin-resistant enterococci and methicillin-resistant Staphylococcus aureus (MRSA), and can significantly improve the susceptibility of methicillin to MRSA. The discovery of compound DB1 provides new ideas and technical support for the preparation of drugs against drug-resistant bacteria.

The application relates to the technical field of surgical biomaterials, and discloses an ultrasonic-responsive piezoelectric hydrogel as well as a preparation method and application thereof.The ultrasonic-responsive piezoelectric hydrogel comprises a conductive hydrogel base body and gallium selenide nanosheets dispersed and loaded in the conductive hydrogel base body; and the conductive hydrogel base body is formed by cross-linking hyperbranched polyglycidol grafted with gamma-polyglutamic acid and polyvinyl alcohol through hydrogen bonding.Under ultrasonic excitation, the gallium selenide nanosheets convert mechanical energy into a microelectric field through a piezoelectric effect and synchronously catalyze hydrogen production from water / glucose, while releasing gallium ions, which synergistically play multiple therapeutic roles: the microelectric field promotes fibroblast proliferation, migration and vascular regeneration, hydrogen gas removes active oxygen in an oxidative stress environment and improves a diabetic wound microenvironment, and the gallium ions realize efficient antibacterial effect on methicillin-resistant Staphylococcus aureus by interfering with bacterial iron metabolism, thereby constructing a response-type treatment system integrating antibacterial effect, antioxidant effect and healing promotion.

Disclosed are compositions, methods and systems for detecting MRSA, for example MRSA nasal colonization. In certain embodiments, the methods use bacteriophage-based amplification of the signal in detection of bacteria and other microorganisms to detect MRSA. The methods for detecting MRSA may include preparing an assay comprising a selective agent and a cocktail comprising at least two different types of recombinant bacteriophages, incubating the sample in the assay, capturing an indicator protein product, and detecting an indicator protein product produced by the recombinant bacteriophage, wherein positive detection of the indicator protein product indicates that MRSA is present in the sample.

The application discloses a natural product 3-methylcarbazole derivative with a general formula B and a preparation method and application thereof in the technical field of organic chemistry. S. aureus S. epidermidis The derivative has good antibacterial activity, can significantly inhibit Staphylococcus aureus, Staphylococcus epidermidis and methicillin-resistant Staphylococcus aureus (MRSA), and can be used for preparing a drug for treating related bacterial infections.​

The application discloses a synergistic bactericidal composition, comprising the following components: a toona sinensis extract: 16-2048 mu g / mL; a macrolide antibiotic: 0.0625-4096 mu g / mL; the bacteria are staphylococcus aureus or methicillin-resistant staphylococcus aureus, and the macrolide antibiotic is erythromycin or azithromycin. The application further discloses a use of the synergistic bactericidal composition in preparation of a medicine for treating skin and soft tissue infections. The composition provided by the application can convert the traditional macrolide antibiotics such as erythromycin and azithromycin which only have a bacteriostatic effect into a preparation which has clear bactericidal activity on staphylococcus aureus and drug-resistant strains thereof. After the toona sinensis extract is combined with the antibiotic, a synergistic effect is exhibited, and the concentration of the toona sinensis extract and the antibiotic required when the toona sinensis extract and the antibiotic are used alone can be greatly reduced.

A method for screening antimicrobial combination drug compositions based on a conditional diffusion model and its application include the following steps: First, an experimental dataset is constructed containing single-drug and pairwise combination antimicrobial measurement data of antibiotics, metalloids, and antimicrobial peptides. Then, combining multimodal structure / sequence characterization and concentration-related phenotypic information, generative modeling and discriminative scoring are integrated to explore and screen the antimicrobial combination space. Candidate combinations are initially screened under Staphylococcus aureus Newman strain, and then further verified in methicillin-resistant Staphylococcus aureus to see if their antimicrobial advantage can be retained under drug-resistant conditions. This invention organically combines the LightGBM scorer with the conditional diffusion model generation mechanism to construct a closed-loop system of generation, evaluation, and screening. This not only improves the diversity and exploratory nature of combinations but also enhances screening efficiency and accuracy, providing high-quality candidate combinations for subsequent experimental verification.

This invention discloses a poly-T double-stranded chimeric DNA-copper nanoparticle and its synthesis method, belonging to the field of DNA-metal nanomaterials technology. This invention uses a poly-T double-stranded DNA chimeric structure as a template to construct a DNA tetrahedron. Copper ions are reduced in situ with ascorbic acid to prepare DNA-copper nanoparticles exhibiting both fluorescence and peroxidase-like activity. The DNA tetrahedron template is formed by the self-assembly of four single-stranded DNA strands, containing six double-stranded edges and four single-stranded vertices. Two of the single strands are poly-T sequences, and two are aptamer sequences targeting bacterial cell wall peptidoglycan. The prepared DNA-copper nanoparticles emit characteristic fluorescence under 340 nm excitation and exhibit peroxidase-like activity. This material is used for bacterial detection and eradication, with minimum inhibitory concentrations (MICs) of 25 nM and 5 nM against *Escherichia coli* and methicillin-resistant *Staphylococcus aureus*, respectively. 3 Up to 10 8 The ability to detect Escherichia coli within the CFU / L concentration range demonstrates its potential for immediate clinical antibacterial applications.

The application discloses a photo-activated antibacterial agent semi-amine compound and a preparation method and application thereof, and belongs to the technical field of organic synthesis chemistry. In view of the technical problems of poor stability of semi-amine compounds in the prior art, unexplored biological activity, limited selection of clinical drugs for resisting MRSA and other drug-resistant bacteria and the like, the application realizes a double breakthrough of semi-amine compounds in stability and biological activity by innovatively modifying a traditional coumarin structure. In addition, the application also provides a preparation method and spectral property data of the compound, and discloses a new use of the compound in preparing a drug for preventing and / or treating infectious diseases caused by methicillin-resistant Staphylococcus aureus (MRSA) and other gram-positive drug-resistant bacteria. The in-vitro antibacterial experiment results show that a representative compound of the application has excellent antibacterial activity on MRSA strains and exhibits a unique ability of inhibiting biofilm formation.

This invention discloses the application of a combined acoustic / optical technology in enhancing the inhibitory effect of MOFs complexes on drug-resistant bacteria, belonging to the field of pharmaceutical technology. The MOFs complex is a composite material, ZIF-8@PDA, with ZIF-8 as the organic framework and PDA coated on its surface. This invention selected methicillin-resistant Staphylococcus aureus (MRSA) as the research object, and its inhibitory effect was measured by the acoustic / photodynamic activity of the material. The results show that the inhibitory effect of ZIF-8@PDA combined with acoustic / optical technology on drug-resistant bacteria is significantly better than that of ZIF-8@PDA complex alone under light irradiation or ultrasound alone. This method, as a potential antibacterial approach, has certain guiding significance for clinical inhibition of drug-resistant bacterial infections.

The application discloses a multimodal antibacterial biological heterojunction, a preparation method and application thereof, and comprises the following steps: S1, preparing a dispersion liquid of single-layer Ti3C2 nanosheet; S2, preparing Fe and Cu doped metal organic framework material: FeCu-MOF; S3, compounding the single-layer Ti3C2 nanosheet and the FeCu-MOF to prepare the multimodal antibacterial biological heterojunction. The MXene / FeCu-MOF heterojunction is innovatively constructed, the band structure and near-infrared plasmonic effect are regulated, the directional transfer of electric charges is effectively promoted, the chemical dynamic therapy (CDT) and photodynamic therapy (PDT) effects are significantly enhanced, and the synergistic effect with photothermal therapy (PTT) is generated. The application designs a new multimodal non-antibiotic treatment strategy, which can realize efficient killing and removal of methicillin-resistant Staphylococcus aureus.

The present application relates to a sophora japonica carbon dot and a preparation method and application thereof, the preparation method of the sophora japonica carbon dot is that after sophora japonica is crushed and sieved, the sophora japonica is uniformly mixed with ethylenediamine and purified water, the mixture is obtained after ultrasonic treatment, the mixture is subjected to heating treatment, after the reaction is finished, cooling to room temperature, and then centrifugation, filtration, dialysis and freeze-drying are carried out to obtain the sophora japonica carbon dot. The present application uses traditional Chinese medicinal material sophora japonica as a carbon source, and prepares the sophora japonica carbon dot by using a one-step hydrothermal method process, has the characteristics of easy synthesis, good water solubility, high biological safety and the like, compared with sophora japonica water extract, the sophora japonica carbon dot has significant antibacterial properties, and has good antibacterial effects on escherichia coli, staphylococcus aureus and methicillin-resistant staphylococcus aureus, especially on methicillin-resistant staphylococcus aureus, and the sophora japonica carbon dot prepared by the present application also has antioxidant properties and can improve ulcerative colitis.

The application discloses application of total flavones of radix scutellariae combined with tetracycline antibiotics in preparation of products against staphylococcus aureus, and belongs to the technical field of medicines. The application provides an antibacterial composition containing total flavones of radix scutellariae and tetracycline antibiotics, aiming at the problems of high drug resistance rate and great toxic side effects of tetracycline antibiotics in the treatment of staphylococcus aureus and methicillin-resistant staphylococcus aureus infection. The total flavones of radix scutellariae contain more than 89% of flavones, and contain various active ingredients such as baicalin and baicalein. The tetracycline antibiotics are selected from doxycycline or minocycline. In vitro experiments prove that the combination of the two has a significant synergistic antibacterial effect on staphylococcus aureus and drug-resistant staphylococcus aureus, and the dosage of doxycycline or minocycline can be reduced by 75%, thereby effectively reducing the toxic side effects.

This invention discloses a series of isoflavulol amine copolymers, their preparation methods, and their antibacterial applications. This series of compounds uses fulvulin as the parent compound, converting fulvulin into isoflavulin. Then, isoflavulin, as the lipophilic moiety, is linked to various aliphatic amines and heterocyclic amines via alkyl linkages of different lengths, synthesizing a series of isoflavulol amine copolymers. Further structural optimization can be performed by introducing methyl groups at the nitrogen atom site to form nitrogen cations. The isoflavulol amine copolymers prepared by this invention exhibit good antibacterial effects against Staphylococcus aureus ATCC 29213 and various methicillin-resistant Staphylococcus aureus (MRSA), and possess advantages such as good water solubility, low biotoxicity, and high yield, showing promise for further development into semi-synthetic antibacterial drugs for clinical use.

This invention discloses a method for preparing a photocatalytic antibacterial composite material; the method involves using Sn... 4+ / Ga ND reacts with an aqueous solution of sodium carboxymethyl cellulose (CMC) and undergoes hydrothermal sulfidation to induce the formation of tin vacancies. Single-atom Mo is then assembled at these tin vacancies using an impregnation method combined with heat treatment to obtain SAMo-SnS2 / Ga ND powder. Simultaneously, using natural corn stalk cellulose as a substrate, it is dissolved in the ionic liquid BmimCl. ATBPC monomers are grafted onto the cellulose molecular chain using RAFT controlled graft polymerization technology to prepare a cellulose-based cationic polyelectrolyte PATBPC-co-Cellulose solution. The PATBPC-co-Cellulose solution is then blended with the SAMo-SnS2 / Ga ND powder and regenerated with ether to form a composite antibacterial material. This composite material has shown high efficiency and long-lasting bactericidal activity in antibacterial experiments against multidrug-resistant Escherichia coli and methicillin-resistant Staphylococcus aureus under both light and dark conditions, demonstrating promising application prospects.

The application discloses drug-resistant bacteria-resistant bone cement containing polypeptides and belongs to the technical field of medical materials. The novel bone cement obtained by adding polypeptides with drug-resistant bacteria resistance activity has good antibacterial property and biocompatibility, can effectively kill drug-resistant bacteria, and the drug-resistant bacteria are methicillin-resistant Staphylococcus aureus.

This invention provides a novel peptide adjuvant CAL185, its composition, and its application. The peptide adjuvant CAL185 and its series derivatives have the following sequences: X0-X1-X2-X3-X4-X5-X6-X 7‑ X8-X9-X 10 -X 11 -X 12 The non-ribosomal peptide adjuvant of this invention can bind to penicillin-binding protein 2a, targeting its allosteric site, and assisting β-lactam antibiotics (such as penicillins, cephalosporins, and carbapenems) in binding to the active site of penicillin-binding protein 2a, thereby enhancing their original low affinity, blocking cell wall synthesis, reversing drug resistance, and exerting a potent bactericidal effect. The peptide adjuvant of this invention, through synergy with the aforementioned antibiotics, exhibits potent bactericidal activity against clinically common methicillin-resistant Staphylococcus aureus, and holds promise for further development into a novel anti-infective drug adjuvant.

The application discloses an oxazolidinone compound and a synthesis method and application thereof, which couples a compound with a structural formula IV and a compound with a structural formula V under the action of a catalyst to obtain a compound with a structural formula II, and then phosphorylates the compound with the structural formula II to obtain a compound with a structural formula I. 1 is methyl, ethyl, propyl or cyclopropyl, ethenyl; R 2 is F; R 3 is F, CH3, C2H5, CF3, CHF2, CH2F or cyclopropyl; X is chlorine (Cl), bromine (Br), iodine (I), substituted boric acid or substituted boric acid ester. The compound has strong bacteriostatic activity on methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus.

This invention discloses the application of UV-regulated Fe / Lyz@CDs nanozymes in fruit preservation. Using lysozyme and diethylenetriaminepentaacetic acid as precursors, Fe-doped lysozyme-based carbon dot (Fe / Lyz@CDs) nanozymes were prepared via a simple hydrothermal method. The Fe / Lyz@CDs nanozymes exhibit UV-responsive peroxidase (POD) and superoxide dismutase (SOD)-like catalytic properties. Fe / Lyz@CDs catalyze the generation of reactive oxygen species (ROS), which are beneficial for *Escherichia coli* (E. coli). E.coli Staphylococcus aureus ( S.aureus Fe / Lyz@CDs exhibits significant bactericidal effects against four typical foodborne pathogens: multidrug-resistant Escherichia coli (AREC) and methicillin-resistant Staphylococcus aureus (MRSA). Fe / Lyz@CDs is dispersed in a polyvinyl alcohol (PVA) matrix to prepare Fe / Lyz@CDs-PVA film. Using this film for mango preservation can maintain the quality of mangoes and extend their storage time.

This invention provides a chitosan oligosaccharide-octyl gallate covalent conjugate, its preparation method, and its applications. The chitosan oligosaccharide-octyl gallate covalent conjugate is obtained by covalently grafting octyl gallate onto the chitosan oligosaccharide molecular chain. The preparation method includes: mixing an ethanol solution of octyl gallate and an aqueous solution of chitosan oligosaccharide, adding ascorbic acid, and stirring under an inert gas atmosphere; adding hydrogen peroxide to initiate the reaction, and immediately subjecting the mixture to ultrasonic treatment to obtain the reaction solution; transferring the solution to a dialysis bag for purification, followed by freeze-drying. The covalent conjugate of this invention exhibits high grafting efficiency and good water dispersibility. Under external light stimulation, it can generate reactive oxygen species, demonstrating a scavenging effect against methicillin-resistant Staphylococcus aureus (MRSA) and its biofilms. Simultaneously, it exhibits good biocompatibility with mammalian cells, showing broad application prospects in the field of anti-infective biomaterials.

This invention relates to a novel methicillin-resistant Staphylococcus aureus (MRSA) bacteriophage and its applications. The invention provides a methicillin-resistant MRSA bacteriophage SP01, which is identified as a new species. This phage possesses highly efficient lytic ability against MRSA and has a broad host spectrum, showing sensitivity to multiple clinical isolates. Furthermore, phage SP01 exhibits excellent environmental stability, maintaining good activity within a temperature range of 4–40°C and a pH range of 7–11. In summary, this invention allows for the preparation of drugs, disinfectants, and bactericides for treating MRSA infections using phage SP01, addressing the problem of conventional antibiotic ineffectiveness and demonstrating broad application prospects.

The application discloses an enzyme-triggered self-assembly peptide hydrogel, a preparation method and application thereof, and belongs to the technical field of biomedical materials. The hydrogel comprises a peptide precursor Nap-YY(p)Y, an antibacterial compound daphnodorin and alkaline phosphatase; the peptide precursor is dephosphorylated under the action of the alkaline phosphatase, self-assembled to form a nanofiber network structure, and encapsulates the daphnodorin in the network structure. The hydrogel has a short gelation time (3-10 minutes), a moderate mechanical strength (a storage modulus G' of 130-170 Pa), a high encapsulation rate (greater than or equal to 75%), presents a biphasic release characteristic in a simulated wound liquid, and can maintain an effective therapeutic concentration for more than 7 days. The hydrogel has a significant synergistic killing effect on methicillin-resistant Staphylococcus aureus and a biofilm thereof, can effectively reduce a bacterial load of a wound and accelerate wound healing.

This invention relates to the field of pharmaceutical synthesis technology, specifically disclosing a 1,3-diamino-7H-pyrrolo[3,2-f]quinazoline derivative and its general structural formula; it also discloses the preparation method and application of this derivative. This 1,3-diamino-7H-pyrrolo[3,2-f]quinazoline derivative exhibits good inhibitory and bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA) and can be used to prevent and treat diseases caused by MRSA infection. Preferably, these diseases include, but are not limited to, pneumonia, urinary tract infections, bacteremia, sepsis, and suppurative arthritis caused by MRSA in humans and animals. The beneficial effects of this invention are: the 1,3-diamino-7H-pyrrolo[3,2-f]quinazoline derivative of this invention is obtained through a four-step reaction, the preparation method is simple, and the reaction conditions are mild; it has good inhibitory and bactericidal activity against MRSA and can be used to prevent and treat diseases caused by MRSA infection.

This invention discloses a photosensitizing lipid nanoparticle based on a multi-resonance core framework, its preparation method, and its antibacterial application, belonging to the field of biomedical materials technology. The photosensitizer structure in this invention is characterized by a multi-resonance fused-ring boron nitrogen as the molecular core framework, with a cleverly introduced triazine electron-withdrawing group on the periphery. This enhances the stretching vibration of the molecule, increases its collision probability with oxygen molecules, accelerates the electron transfer process, and thus promotes the generation of type I reactive oxygen species (ROS). The lipid nanoparticles BN-TRZ@NPs prepared by ultrasound have an absorption wavelength in the range of 550-680 nm. Under white light irradiation, BN-TRZ@NPs can efficiently generate type I ROS (superoxide anion radicals) and exhibit excellent sterilization effects against methicillin-resistant Staphylococcus aureus (MRSA). This invention is the first to apply a photosensitizer based on a multi-resonance structure to biomedical research in the field of antibacterial applications, providing a new approach for the biomedical application of next-generation biotherapeutic photosensitizer molecules.

This invention relates to the field of biomedical technology, and in particular to the application of TarA inhibitors in the preparation of antibacterial potentiators for enhancing the efficacy of β-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA) infections. Through virtual screening, target validation, and in vitro and in vivo pharmacodynamic experiments, this invention confirms that the natural product norzelamin is an effective TarA inhibitor and antibacterial potentiator. Experiments show that norzelamin has a synergistic effect when used in combination with various β-lactam antibiotics. In a mouse model of MRSA-infected pneumonia, the combination of norzelamin and cefotaxime sodium significantly reduced pulmonary bacterial load, alleviated systemic inflammatory response, and improved lung tissue pathological damage compared to monotherapy. This invention provides a novel synergistic treatment strategy and candidate compound for overcoming MRSA resistance in clinical practice, and has significant development value and application prospects.

This invention discloses a truncated pleurotin derivative containing a long-chain quaternary ammonium salt side chain, its preparation method, and its applications, belonging to the field of medicinal chemistry. The invention provides a truncated pleurotin derivative containing a long-chain quaternary ammonium salt side chain. Using truncated pleurotin as the parent nucleus, its structure was modified, and extensive bioactivity screening was conducted to synthesize a novel truncated pleurotin quaternary ammonium salt antibiotic with antimicrobial activity. In vitro antibacterial activity experiments demonstrated that the truncated pleurotin derivative containing a long-chain quaternary ammonium salt side chain synthesized in this invention has excellent antimicrobial activity, especially against methicillin-resistant Staphylococcus aureus (MRSA).