119 results about "Methicillin resistant Staphylococcus" patented technology

The invention relates to an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID No. 1. The antibacterial peptide has excellent broad-spectrum antibacterial activity, for example, the antibacterial peptide has antibacterial activity on bacteria of the genus Staphylococcus (Staphylococcus), the genus Acinetobacter (Acinetobacter) and the genus Bacillus (Bacillus). Under the background that the global bacterial drug resistance problem is increasingly severe and traditional antibiotics gradually lose efficacy on multi-drug-resistant bacteria, a new treatment means is provided for treating bacteria such as methicillin-resistant staphylococcus aureus, carbapenem-resistant acinetobacter baumannii and bacillus subtilis which bring huge threats to public health. And a new possibility is provided for solving the problem that no medicine can be used clinically.

The invention discloses a polyketone compound derived from actinomycetes as well as a preparation method and application thereof, and relates to the technical field of microbial natural product mining and biological medicine, and the key point of the technical scheme is that the invention discloses a novel polyketone compound Strepactone derived from actinomycetes Streptomyces sp. DP0001, and the molecular formula of the novel polyketone compound Strepactone is C25H38O5. The compound is obtained through strain fermentation, ethyl acetate extraction and multi-step chromatographic purification, and the structure is identified through ESI-MS, 1H NMR and 13C NMR. Experiments show that Strepactone has an inhibition effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus and has remarkable inhibition activity on human non-small cell lung cancer A549 cells, the half inhibitory concentration IC50 of the Strepactone is 5.36 mu M, and the Strepactone has no obvious cytotoxic activity on human embryo kidney cells 293T cells at the concentration of 30 mu M. The compound provided by the invention can be used for preparing anti-drug-resistant bacteria drugs and anti-human non-small cell lung cancer drugs, and also provides a structural basis for the development of novel antibacterial and anti-tumor leading drugs.

The invention discloses a co-culture method of methicillin-resistant staphylococcus aureus and pseudomonas aeruginosa, and relates to the technical field of microbiology. Comprising the following steps: preparing materials: preparing methicillin-resistant staphylococcus aureus MRSA, pseudomonas aeruginosa PA and a culture medium; performing strain culture and activation, inoculating MRSA and PA, and performing subculture; preparing a bacterial suspension, and culturing MRSA and PA overnight; adding overnight culture bacterium liquid MRSA and PA into the culture medium, oscillating and uniformly mixing to prepare a bacterium suspension, and diluting for later use; the complete processes of strain preparation, bacterial suspension preparation, direct contact and indirect contact co-culture, drug sensitivity testing and the like are covered, and the interaction between MRSA and PA can be comprehensively researched; indirect contact co-culture is realized by adopting a Transwell insert, and a new method is provided for researching the interaction of two bacteria under the non-direct contact condition.

This invention discloses the application of ferrous ions in the preparation of products for treating bacterial infections, belonging to the field of pharmaceutical technology. Antibacterial activity tests of ferrous ions against Staphylococcus aureus showed strong bactericidal effects, with a survival rate of less than 0.001% against Staphylococcus aureus and less than 0.01% against methicillin-resistant Staphylococcus aureus (MRSA). The use of ferrous ion-containing hydrogels in the treatment of keratitis and skin wound infections significantly reduced the risk of MRSA infection in the lungs and effectively prevented the proliferation and spread of microorganisms in the body, revealing that drugs containing ferrous compounds can be used to treat Staphylococcus aureus infections, including MRSA.

This invention discloses a method for preparing and applying a photothermal nanoreagent targeting bacteria. This invention utilizes Ti3C2T... x Using MXene as a matrix, a photothermal nanoreagent MXene@PDA@Peptide (MPP) was constructed by sequentially modifying the surface of Ti3C2Tx MXene with polydopamine and grafting it with the peptide CAEKA, which has the function of recognizing bacteria. Under the recognition of the peptide CAEKA, the photothermal Ti3C2Tx MXene was targeted. x MXene nanosheets are guided to the surface of bacterial cells, achieving effective sterilization at a relatively low overall temperature of around 50°C (or achieving more efficient sterilization at the same overall temperature level, exhibiting superior sterilization performance compared to pure Ti3C2Tx MXene). This makes them particularly suitable for the preparation of therapeutic drugs for subcutaneous abscesses caused by methicillin-resistant Staphylococcus aureus (MRSA) infection.

The application belongs to the field of antibacterial two-dimensional nanomaterial Mxene catalysts, and particularly relates to a monolayer W@MoS2 nanosheet as well as a preparation method and application thereof. The method comprises the following steps: 1) dissolving water-soluble molybdate and water-soluble tungstate in water to obtain a mixed solution; 2) adding a sulfur source and an organic solvent to the mixed solution, and then performing reaction after mixing, and drying to obtain a powder. The monolayer W@MoS2 nanosheet has a unique two-dimensional planar structure, a large specific surface area, and good optical and electronic properties, and has high light-heat absorption in the near-infrared two region, i.e., the light-heat conversion efficiency is improved. Meanwhile, the peroxidase-like activity of the monolayer W@MoS2 nanosheet material realizes efficient light-heat amplification, can treat methicillin-resistant Staphylococcus aureus infection, and avoids heat therapy damage to normal tissues.

The invention belongs to the technical field of natural antibacterial drugs, and discloses application of cyclovirobuxine D and pharmaceutically acceptable salts thereof in preparation of antibacterial drugs. The invention discloses the effect of cyclovirobuxine D in preparation of antibacterial drugs, and tests prove that cyclovirobuxine D has remarkable antibacterial activity in inhibition of gram-positive bacteria such as staphylococcus aureus, gram-negative bacteria such as escherichia coli and super drug-resistant bacteria such as MRSA (Methicillin Resistant Staphylococcus Aureus).

The invention discloses a method for rapidly and efficiently preparing a series of novel ester group facaplysin derivatives by combining EDCI and HOBT to react with carboxyl facaplysin and directly carrying out ester exchange reaction on an active ester intermediate and alcohol without separation. The invention also discloses application of the derivatives in resisting methicillin-resistant staphylococcus aureus and escherichia coli. The preparation method comprises the following steps that EDCI and HOBT are combined to activate carboxyl facaplysin for reaction, an activated ester intermediate is generated, the activated ester intermediate and alcohol are directly subjected to esterification reaction without separation, and a series of ester-based facaplysin derivatives are obtained. According to the preparation method, ester-based Fascaplysin derivatives with different structures can be rapidly and efficiently constructed, and compared with a traditional method that ester-based carboline derivatives are firstly synthesized and then high-temperature intramolecular cyclization is performed, the time and the raw material cost are greatly saved, the synthesis efficiency is improved, the reaction process is observable and controllable, and the yield is stable. And the synthesized ester group facaplysin derivative has excellent antibacterial activity on methicillin-resistant staphylococcus aureus and escherichia coli.

The application belongs to the technical field of medical materials, and particularly relates to a sonodynamic Ag2O / MoS2 nanocomposite material as well as a preparation method and application thereof. The Ag2O / MoS2 nanocomposite material is prepared by depositing Ag2O on the surface of MoS2 to construct a heterostructure under the catalysis of a surfactant CTAB. The inhibition rate of the Ag2O / MoS2 nanocomposite material prepared by the application to 10 6 CFU / mL of Staphylococcus aureus, Escherichia coli and methicillin-resistant Staphylococcus aureus reaches 95.98% or more.

The invention discloses a photoresponsive antibacterial hydrogel, which comprises a photosensitizer, acrylamide, potassium 3-sulfopropyl methacrylate, chitosan quaternary ammonium salt, an additive and a cross-linking agent, and the structure of the photosensitizer (PD-Se) is shown in the specification. A photosensitizer (PD-Se) is added into hydrogel, the positive charge characteristic of QSA hydrogel remarkably improves the local enrichment concentration of PD-Se in thalli by enhancing the binding force with the bacterial surface, unsaturated fatty acid in a bacterial membrane lipid bimolecular layer is oxidized after photoinduced ROS is generated, guanine bases in a DNA double-helix structure are attacked at the same time, and therefore the bioactivity of the bacterial membrane lipid bimolecular layer is improved. The gel is endowed with photodynamic antibacterial ability, has an excellent sterilizing effect on escherichia coli, staphylococcus aureus and methicillin-resistant staphylococcus aureus, and can be used for preparing hydrogel dressing for wound repair.

The invention belongs to the technical field of medicines, and relates to application of an indole-3-aryl ketone derivative to preparation of a medicine for treating or preventing infectious diseases caused by staphylococcus aureus, in particular to application of the indole-3-aryl ketone derivative to preparation of a medicine for treating or preventing infectious diseases caused by drug-resistant staphylococcus aureus. The chemical structural formula of the indole-3-aryl ketone derivative disclosed by the invention is as shown in a formula I shown in the specification. Results of the invention show that the compound I (namely the indole-3-aryl ketone derivative as shown in the formula I) has strong inhibitory activity on methicillin-resistant staphylococcus aureus ATCC43300, the inhibitory effect is dependent on concentration gradient, the MIC50 value is 27.45 mu g / mL, and the MIC50 value of a positive drug streptomycin sulfate is greater than 200 mu g / mL. Therefore, the indole-3-aryl ketone derivative can be used for treating or preventing infectious diseases caused by the staphylococcus aureus.

Composition for use for the treatment and / or prevention of infections or lesions of the (oral) mucosa, in particular originating from at least one selected the group consisting of: perleche; scarlet fever; pharyngitis; acute necrotising ulcerative mucositis; impetigo contagiosa; chelitis glandularis apostem atosa; gingivostomatitis herpetica; herpes simplex; herpes stabistis recidivans; herpes zoster; hand-foot-mouth; human papilloma virus; HIV; oral candidiasis; oral lichen planus; lichen ruber planus; leukoplakia, erythroplakia; methicillin-resistant staphylococcus aureus; gingivitis / periodontitis; mucositis / peri- implantitis; alveolar osteolytis; wherein the composition comprises or consists of the following components: (a) polyhexanide (PHMB); (b) an organic or inorganic buffer different from a); (c) further additives; (d) optionally water.

The invention provides an application of SX-682 in preparation of a drug for resisting staphylococcus aureus infection, and the SX-682 has a CAS number of 1648843-04-2. According to the technical scheme, the novel medical application of the SX-682 is disclosed, and the SX-682 has better antibacterial activity on staphylococcus aureus and can inhibit formation of biofilms including methicillin-sensitive staphylococcus aureus (MSSA) and methicillin-resistant staphylococcus aureus (MRSA). The SX-682 has no significant toxicity to human cytotoxicity in the bacteriostatic concentration range, and provides key support for subsequent anti-infection application of the SX-682.

The invention discloses application of iso-pasteuretin in preparation of a medicine for treating diseases caused by methicillin-resistant staphylococcus aureus, and belongs to the technical field of biological medicine. According to the present invention, it is identified that the snowfield tea endophytic fungus 202109-Ts-F016 is the new Coprinopsis thamnolicola under coprinus pseudoratus, three monomeric compounds with anti-MRSA activity are separated from the secondary metabolite of the Coprinopsis thamnolicola, the three compounds can be adopted as the candidate inhibitors of the novel antibacterial target FtsZ, and the toxicity of the pasteurestin C and the iso-pasteurestin on the Vero cell is low. The invention provides an important basis and a material basis for the development of coprinus fungus metabolites and anti-MRSA new target drugs.

This invention belongs to the field of biomedical functional materials technology, and relates to an antibacterial and healing-promoting photosensitizer, its preparation method, and its uses. The photosensitizer is a defective MIL-88B@MIL-88C composite material, wherein defective MIL-88B serves as the substrate, and MIL-88C grows on the surface and / or in the pores of the defective MIL-88B. This photosensitizer significantly improves the photogenerated carrier separation efficiency, reduces impedance, enhances photocurrent response, and can efficiently generate reactive oxygen species under light irradiation. Antibacterial experiments show that it has excellent bactericidal effects against Staphylococcus aureus, Escherichia coli, and methicillin-resistant Staphylococcus aureus, and is not prone to inducing drug resistance. Simultaneously, this photosensitizer has good biocompatibility, promotes cell migration, and has significant hemostatic function. Animal models show that it can effectively accelerate the healing of skin defects.

Provided are compositions for controlling the growth of methicillin-resistant Staphylococcus aureus (MRSA). Uses of the compositions, alone or in combination with certain other agents, are also disclosed.

The invention relates to the technical field of biology, in particular to a broad-spectrum bacteriophage lyase mutant, an extraction and purification reagent and a kit. The invention provides a broad-spectrum bacteriophage lyase mutant, an extraction and purification reagent and a kit. A broad-spectrum bacteriophage lyase mutant nucleic acid release liquid containing protein with an amino acid sequence as shown in SEQ ID NO: 1 is adopted; the compound can efficiently act on two important clinical drug-resistant bacteria, namely methicillin-resistant staphylococcus aureus MRSA and vancomycin-resistant enterococcus VRE, at the same time. Through unique molecular design and preparation process optimization, the lyase shows excellent synchronous wall breaking capacity, cell wall destruction can be completed in an extremely short time, and the nucleic acid release efficiency is remarkably superior to that of a traditional method.

The invention discloses a method for rapidly detecting methicillin-resistant staphylococcus aureus and application of the method. According to the invention, a loop-mediated isothermal amplification (LAMP) technology is combined with a naturally extracted luminescent molecule-berberine for rapid detection of methicillin-resistant staphylococcus aureus. The operation process is simple and convenient, the dependence on instruments and equipment can be reduced, the detection cost can be greatly reduced by the luminous Chinese herbal medicine, the result judgment is performed by directly observing the generated fluorescence signal, the visualization and intuition of the detection result are realized, and the operation and result judgment of non-professional personnel are facilitated.

The application discloses a kind of smelly danchang dots and preparation method and application, belong to medical technical field.The preparation method of smelly danchang dot includes: smelly danchang fine powder is dispersed in distilled water, and is stirred pyrolysis on heating table;Pyrolysis product is diluted with distilled water, and supernatant is obtained by centrifugation, filter membrane filtration, dialysis, freeze-drying, to obtain carbon dot.The average particle size of obtained carbon dot is 4.51±1.04nm.The smelly danchang dot prepared in the application shows strong inhibitory activity to a variety of gram-positive bacteria (such as listeria, methicillin-resistant staphylococcus aureus MRSA) and fungi (such as Candida albicans), especially to clinically tricky drug-resistant strains, such as vancomycin-resistant enterococci (VRE) and MRSA effective, can be applied to the preparation of antibacterial drugs, antibacterial dressing, disinfectant or preservative, with the advantages of wide raw material source, simple preparation method, low cost, environment-friendly and the like.

This invention discloses the application of the bacterial fission protein FtsZ inhibitor DB1 in the preparation of drugs against drug-resistant bacteria, belonging to the field of drug development technology. The inhibitor provided by this invention is compound DB1, named 3-(2,8-dibromo-10,11-dihydro-5H-dibenzo[b,f]azapheno-5-yl)-N,N-dimethylpropylamine hydrochloride, with the molecular formula C2. 19 H 24 Br2ClN2. This compound, DB1, exhibits significant inhibitory effects against various drug-resistant bacteria, particularly vancomycin-resistant enterococci and methicillin-resistant Staphylococcus aureus (MRSA), and can significantly improve the susceptibility of methicillin to MRSA. The discovery of compound DB1 provides new ideas and technical support for the preparation of drugs against drug-resistant bacteria.

The thermoelectric material opens up a new way for self-power supply of microelectronic devices, and the combination with the sensor has been significantly developed. However, the application of the thermoelectric material in the field of biosensors is still challenged, which limits the potential of the thermoelectric material in exploring biochemical reactions and promoting the development of biosensors. The invention innovatively provides a detection method based on a silver selenide thermoelectric film, and efficient detection of hydrogen peroxide is realized through photo-thermal-thermoelectric conversion. Compared with a traditional method, the method is short in detection time, low in cost and easy and convenient to operate. Furthermore, the specific detection of streptococcus sanguis and methicillin-resistant staphylococcus aureus is realized by utilizing the characteristics of different bacteria, and a new guiding thought is provided for the diagnosis and treatment of diseases.

Disclosed are compositions, methods, and systems for detecting MRSA, e.g., nasal colonization by MRSA. In certain embodiments, the methods use bacteriophage-based amplification of signals in the detection of bacteria and other microorganisms to detect MRSA. A method for detecting MRSA can include preparing an assay containing a selection agent and a cocktail containing at least two different types of recombinant bacteriophage, incubating a sample in the assay, capturing an indicator protein product, and detecting the indicator protein product produced by the recombinant bacteriophage, wherein positive detection of the indicator protein product indicates the presence of MRSA in the sample.

The invention discloses an application of forsythiaside A in preparation of an inhibitor for treating methicillin-resistant staphylococcus aureus Stp1. Firstly, an Stp1 protein is purified for an Stp1 phosphatase activity experiment, and it is found that the protein activity is reduced along with increase of the drug concentration, and the IC50 value is 2.673 micrograms / mL. Then, the forsythiaside A with different concentrations and the USA300 strain are co-cultured, and it is found that the forsythiaside A cannot inhibit growth of the USA300 strain. In addition, in-vitro experiments show that forsythiaside A shows a good cell protection effect, and staphylococcus aureus mediated cell damage is effectively relieved; in-vivo experiments show that the forsythiaside A relieves infection caused by methicillin-resistant staphylococcus aureus, and lesions of main organs (liver, kidney and lung) of mice are improved. Therefore, the forsythiaside A does not cause the generation of bacterial drug resistance by targeting the staphylococcus aureus Stp1, and has important significance in the aspect of treating infection caused by methicillin-resistant staphylococcus aureus.

The invention discloses a composite nanoprobe for detecting methicillin-resistant staphylococcus aureus, a kit and application of the composite nanoprobe and the kit, and belongs to the technical field of biology. The composite nano probe is prepared from a methicillin-resistant staphylococcus aureus (MRSA) nucleic acid aptamer modified by a fluorescent dye Cy7 and gold-palladium-platinum nano particles. The invention develops a fluorescent sensing detection method for sensitively and specifically recognizing and rapidly detecting MRSA based on the principle that a nucleic acid aptamer specifically recognizes the MRSA and the nano-metal surface energy transfer synergistic inner filter effect between an energy transfer donor and an energy transfer acceptor. The method is high in sensitivity, the detection limit is as low as 8 CFU / mL, and the detection linear range is wide and is 101-106 CFU / mL. The method can realize rapid and high-specificity identification and detection of MRSA within 55min, is suitable for rapid detection of drug-resistant bacteria, and has wide application potential.

The application relates to the technical field of surgical biomaterials, and discloses an ultrasonic-responsive piezoelectric hydrogel as well as a preparation method and application thereof.The ultrasonic-responsive piezoelectric hydrogel comprises a conductive hydrogel base body and gallium selenide nanosheets dispersed and loaded in the conductive hydrogel base body; and the conductive hydrogel base body is formed by cross-linking hyperbranched polyglycidol grafted with gamma-polyglutamic acid and polyvinyl alcohol through hydrogen bonding.Under ultrasonic excitation, the gallium selenide nanosheets convert mechanical energy into a microelectric field through a piezoelectric effect and synchronously catalyze hydrogen production from water / glucose, while releasing gallium ions, which synergistically play multiple therapeutic roles: the microelectric field promotes fibroblast proliferation, migration and vascular regeneration, hydrogen gas removes active oxygen in an oxidative stress environment and improves a diabetic wound microenvironment, and the gallium ions realize efficient antibacterial effect on methicillin-resistant Staphylococcus aureus by interfering with bacterial iron metabolism, thereby constructing a response-type treatment system integrating antibacterial effect, antioxidant effect and healing promotion.

The invention relates to the technical field of water treatment materials, and discloses a nano-catalytic antibacterial filter material for a direct water dispenser, which comprises the following components in percentage by mass: 8-20% of broad-spectrum nano-antibacterial component, 55-85% of base material, 4-10% of binder, 2-5% of dispersant and 2-6% of assistant, the broad-spectrum nano antibacterial component is Ag-TiO / ZnO-CuO quaternary composite nanoparticles, and the mass ratio of Ag to TiO to ZnO to CuO is 1: (2-4): (1-3): (0.5-1.5). According to the nano catalytic antibacterial filter material and the preparation method thereof, the inhibition and killing rates on gram-negative bacteria (escherichia coli, pseudomonas aeruginosa and klebsiella pneumoniae), gram-positive bacteria (staphylococcus aureus, bacillus subtilis and methicillin-resistant staphylococcus aureus) and fungi (candida albicans and aspergillus niger) are all greater than or equal to 99.9%.

The invention belongs to the technical field of biological medicine, and discloses a prodigiosin (PG) and tannic acid (TA) self-assembled carrier-free nano-particle as well as preparation and application thereof. The particles are formed by PG and TA through nano-precipitation self-assembly, and the particle size is 30-50 nm. The preparation method comprises the following steps: respectively dissolving PG and TA in DMSO, mixing and stirring according to a molar ratio of (5-10): 1, dropwise adding ddH2O, carrying out ultrasonic treatment, dialyzing to remove a solvent, centrifuging, taking precipitate, and freeze-drying to obtain powder. The nanoparticles can efficiently kill methicillin-resistant staphylococcus aureus and other multi-drug-resistant bacteria, remove excessive ROS, inhibit TNF-alpha, IL-1beta and IL-6 and promote expression of IL-4, IL-10 and TGF-beta, so that infected skin flap necrosis is relieved, skin repair is promoted, and the nanoparticles have good biological safety and can be used for preparing antibacterial and infected wound treatment drugs.

This application discloses a method for preparing amide derivatives containing bisthiazole according to general formula III in the field of organic chemistry, wherein the substituent R1 is phenyl, substituted phenyl, thiazolyl, cyclopropyl, or an alkyl group of 1 to 3 carbons; or R2 is an alkyl group of 1 to 3 carbons. The compounds provided in this application possess potent antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA), and they show broad application prospects in treating infectious diseases caused by these bacteria. Furthermore, these compounds can be used in combination with other antibacterial active substances to further enhance antibacterial effects or broaden the antibacterial spectrum, providing a more effective means of treating complex infections.

Disclosed are compositions, methods and systems for detecting MRSA, for example MRSA nasal colonization. In certain embodiments, the methods use bacteriophage-based amplification of the signal in detection of bacteria and other microorganisms to detect MRSA. The methods for detecting MRSA may include preparing an assay comprising a selective agent and a cocktail comprising at least two different types of recombinant bacteriophages, incubating the sample in the assay, capturing an indicator protein product, and detecting an indicator protein product produced by the recombinant bacteriophage, wherein positive detection of the indicator protein product indicates that MRSA is present in the sample.

The invention belongs to the technical field of biological medicine, and relates to sargassum polysaccharide as well as a preparation method and application thereof. The uniform acidic polysaccharide WZSP-2 is separated and purified from the Weizhou Sargassum through hot water extraction, ethanol precipitation, protein and color removal, anion exchange column and gel column chromatography and other methods, and the physicochemical properties, monosaccharide composition and structural morphology of the Weizhou Sargassum are systematically characterized. In-vitro experiments show that the WZSP-2 has remarkable DPPH, ABTS and hydroxyl radical scavenging capacity and total reducing power, can effectively inhibit the activity of alpha-amylase and alpha-glucosidase, can be combined with glycocholate and taurocholate, and has a bacteriostatic effect on methicillin-resistant staphylococcus aureus (MRSA) at the same time. The sargassum polysaccharide provided by the invention integrates antioxidant activity, hypoglycemic activity, hypolipidemic activity and antibacterial activity, provides a new source for developing multifunctional natural products, and has a good application prospect in the fields of functional food, health care products and medical auxiliary materials.