The invention relates to a high-sensitivity testing method capable of simultaneously implementing measurement of multiple negative ions. The analysis objects not only comprise Cl<->, NO2<->, NO3<-> and SO4<2->, but also comprise easily polarized ions Br<->, I<-> and S2O3<2->. A negative ion analysis column AS9-HC and a protection column AG9-HC are adopted together, the washing solution comprises Na2CO3 and NaHCO3 in a concentration ratio of 9mm: 1mm, the flow rate of the washing solution is 0.9 to 1.1ml/min, the detector is an electrical conductivity detector, the current of a suppressor is 88mA, the temperature of the columns and the temperature of a detection cell are 28 to 32 DEG C, and the used quantitative ring is a polyether-ether-ketone (PEEK) tube with inside diameter of 0.030in, outside diameter of 1/16in and length of 7m. The lower detection limits of the ions are low and reach nano scale, namely the lower detection limit of Cl<-> is 0.61 nanometer, the lower detection limit of the NO2<-> is 0.94 nanometer, the lower detection limit of the NO3<-> is 0.93 nanometer, the lower detection limit of the SO4<2-> is 0.81 nanometer, the lower detection limit of the Br<-> is 0.90 nanometer, the lower detection limit of the I<-> is 2.48 nanometers and the lower detection limit of the S2O3<2-> is 1.84 nanometers. The method is simple and convenient for operation, and can be used for implementing high-sensitivity measurement of multiple negative ions indoors or outdoors.