The invention provides a threshold voltage detection method of an OLED (organic light emitting diode) driver thin film transistor. According to the method, by setting data signals, two different data voltages are offered, so that two different gate-source voltages are formed by virtue of the driver thin film transistor; then, by virtue of an external detection processing circuit, currents, passing through the driver thin film transistor under two different gate-source voltages, are detected; and by virtue of the two gate-source voltages, two current data and a calculating formula based on a driver thin film transistor current formula, a threshold voltage of the OLED driver thin film transistor is calculated by virtue of a central processing unit; therefore, the threshold voltage of the driver thin film transistor of each pixel in an OLED display device can be accurately acquired, and subsequently, a threshold voltage compensating effect of the OLED driver thin film transistor is improved and OLED display quality is enhanced.