The application provides a solution
refractive index real-time detection method based on amplification imaging and
machine vision, which comprises the following steps: step A, illumination light emitted by an illumination
light source is irradiated to a
microscopic scale, and forms light carrying
microscopic scale information, and then sequentially passes through a sample
flow cell and a hollow glass block, and is emitted to an
image sensor; step B, the emitted light is imaged on the
image sensor, the image information of the
microscopic scale on the
image sensor is recorded, the image sensor is driven to displace through a focusing
algorithm, until the imaging of the microscopic scale is a clear image, and the axial position of the image sensor is recorded, and the solution
refractive index is analyzed according to the relationship between the axial position of the image sensor and the solution
refractive index; step C, the
magnification of the object image of the microscopic scale on the imaging
system is analyzed through the imaging size of the microscopic scale on the image sensor, and the solution refractive index is inversely calculated, and the solution refractive index obtained in step B is compared, and the redundancy of
system measurement is improved.