279 results about "Genetic testing" patented technology

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The invention relates to a primer group, a kit and a method for detecting an ATXN3 gene (CAG) n trinucleotide repetition number, and belongs to the technical field of gene detection. The technical problem to be solved by the invention is to provide the primer group for detecting the n trinucleotide repetition number of the ATXN3 gene (CAG). The primer group comprises a first primer pair and a second primer pair, a forward primer sequence of the first primer pair is as shown in SEQ ID NO. 1, and a reverse primer sequence of the first primer pair is as shown in SEQ ID NO. 2; a forward primer sequence of the second primer pair is as shown in SEQ ID NO. 3, and a reverse primer sequence of the second primer pair is as shown in SEQ ID NO. 4. Through the design of the two pairs of primers, complementary verification is achieved, the detection result is comprehensive and reliable, the technical defects of first-generation sequencing and second-generation sequencing on long-fragment and high-GC-content repetitive sequence detection are overcome, the detection cost is low, the detection period is short, and the primer pair is suitable for clinical detection service.

The invention relates to the technical field of gene detection, and discloses a mycobacterium tuberculosis drug resistance gene detection data analysis system which comprises a data import module, a signal correction module, a feature extraction module, a multi-stage interpretation module, a result fusion module and a report generation module. The system performs baseline drift correction, abnormal point detection and nonlinear normalization on a fluorescence intensity value output by a detector, extracts multi-dimensional features such as peak intensity, a mutation ratio, a consistency coefficient and an in-batch difference index, and constructs a dynamic threshold function to realize wild type and mutation type layered judgment. A drug resistance risk conclusion is generated through drug resistance characteristic matrix mapping, a standardized detection report is output, and intelligent analysis of a detection result and automatic report generation are achieved. Through multi-stage signal correction, multi-dimensional feature extraction and dynamic threshold interpretation, intelligent analysis and standardized report output of a mycobacterium tuberculosis drug resistance gene detection result are realized, and data judgment accuracy and clinical application efficiency are improved.

The application belongs to the technical field of gene detection, and particularly relates to a biological sample nucleic acid rapid extraction method and kit based on a magnetic bead method. The method comprises the following steps in sequence: S1, liquefying a biological sample by using a liquefying solution; the liquefying treatment is to add 1-4 times the volume of the liquefying solution to the biological sample, shake and mix, and then place at room temperature for 15-30 minutes; the liquefying solution comprises guanidine salt, potassium salt and / or sodium salt, Tris-HCl, EDTA and isopropyl alcohol; S2, lysing the biological sample; adding a lysing solution and magnetic beads to the system obtained in S1, and heating at 90-100 DEG C for 3-20 minutes to lyse the biological sample; S3, cleaning the biological sample; and S4, eluting the nucleic acid. The method does not need centrifugal separation in the operation process, and can realize automatic and efficient extraction of the nucleic acid.

The invention discloses a method and equipment for automatically generating a gene detection report. The method comprises the following steps: acquiring gene detection result data; forming cue word input data of a pre-trained report generation model based on the gene detection result data; and based on the cue word input data, outputting a target interpretation report through the report generation model.

The utility model relates to the field of medical detection, provides a sample storage and treatment device for gene detection, and aims to solve the problems of inconvenience in storage, easiness in omission and risk of pollution of DNA (deoxyribonucleic acid) samples during treatment in the prior art, and the sample storage and treatment device comprises a shell, a puncher, a punching die and a storage positioning mechanism, the punching die is arranged on the shell; the storing and positioning mechanism is detachably connected to the punching die and used for storing and positioning the dried blood spot collection cards during punching. The storage positioning mechanism can store a plurality of dried blood spot collection cards after sampling and can also perform positioning; when the blood spot needs to be punched and cut out, the storage positioning mechanism is directly installed on the shell, and the puncher is used for punching. And through the matched design of the storage positioning mechanism and the punching die, the dried blood spot collection cards do not need to be sequentially taken out for punching during sample punching treatment, pollution to dried blood spot collection card samples for Rh blood group gene detection is effectively reduced, and more convenience is achieved. The method is especially suitable for large-scale Rh blood group gene screening.

The application relates to the technical field of gene detection, and particularly discloses a kit for human autosomal miniSTR typing and application thereof. The kit comprises 10 pairs of STR primer groups for amplifying 10 miniSTR loci, and the 10 miniSTR loci are D3S1358, D21S11, D18S51, D7S820, D15S131, D15S12, D15S146, D16S539, D5S818 and TPOX. The kit can successfully perform STR typing on highly degraded DNA samples, and can be used as a supplement of traditional STR reagents.

The utility model provides a cell centrifugal separation device for gene detection, include: shell main part and cooperation seal's sealing cover, be provided with the inner bag in the shell main part, the inner bag bottom cooperation is provided with speed reducer, speed reducer output end extends to the inner bag, speed reducer output end top cooperation is provided with centrifugal separation main part, by this, the utility model has the advantages that, the device simple structure, stability is good, is favorable to cell centrifugal separation, the shell main part has the temperature, the rotating speed and the time required for cell centrifugal separation, and the speed motor is the most power source, realizes synchronous locating disc rotation through transmission, the test tube positioning frame that carries cell test tube moves synchronously with synchronous locating disc synchronously, and the centrifugal angle is adjusted adaptively with the rotating speed difference, reaches the stable cell centrifugal separation effect.

The invention relates to a full-length sequence amplification primer, an amplification method and a three-generation sequencing method of an HLA-I antigen gene, and belongs to the field of gene detection. The HLA-I antigen gene is subjected to PCR (Polymerase Chain Reaction) amplification by using 8 pairs of characteristic amplification primers. And each gene can be subjected to high-yield specific amplification on a full-length sequence containing complete intron and exon regions only through one round of amplification and enrichment, and 7.8 Kb long-fragment genes can be captured once at most. The experimental deviation caused by simultaneous amplification of a single gene by using a plurality of pairs of segmented primers and the indirect error caused by gene splicing are effectively avoided. The method comprises the following steps: carrying out quality inspection and purification on amplicon PCR (Polymerase Chain Reaction) products, mixing samples, constructing an HLA-I type antigen gene library, carrying out accurate and complete sequencing on the full-length sequence of the HLA-I type antigen gene by adopting a three-generation PacBio Sequel II platform, and ensuring that the Hifi reads base accuracy can reach 99% or above. The reading of HLA-I type antigen gene full-length sequence variation information and the haplotype analysis at a high resolution level can be realized.

This invention discloses a gene detection method and gene detection cartridge based on magnetic control technology, comprising: setting a capillary magnetic bead moving channel, a sample processing chamber, and an amplification chamber within the detection cartridge; the sample processing chamber and the amplification chamber are separated into one or more cleaning zones by a high-viscosity liquid substance or a thermoplastic hydrophobic substance through the capillary magnetic bead moving channel; adding the sample to the sample processing chamber, mixing the sample with sample processing reagents and nano-magnetic beads, adsorbing nucleic acids by the nano-magnetic beads, and having an external magnet adsorb the nano-magnetic beads and drive them along the capillary magnetic bead moving channel sequentially through the cleaning zones into the amplification chamber; eluting the nucleic acids in the amplification chamber and performing an amplification reaction; and detecting the amplified fluorescence signal through an external optical device to achieve integrated nucleic acid extraction, amplification, and detection. This invention improves the integration of the detection cartridge, reduces manufacturing costs, and increases product yield through optimized design of capillary liquid isolation and flow channel degassing technology.

The application belongs to the field of biotechnology detection, and particularly relates to a multiplex PCR detection kit for identifying and distinguishing chicken pullorum salmonella and chicken typhoid salmonella. The kit comprises stn gene detection primers, I137_14445 gene detection primers and ybgL gene detection primers. The kit can quickly, high-throughput and accurately identify and distinguish chicken pullorum salmonella and chicken typhoid salmonella, can replace traditional salmonella serological typing and biochemical identification and other complex procedures, and provides a simple, rapid, reproducible new method for monitoring and laboratory diagnosis of chicken pullorum salmonella and chicken typhoid salmonella.

The application relates to the field of second-generation sequencing, in particular to an ultrahigh-throughput multiplex PCR amplicon capture method for second-generation sequencing. The NGS capture method comprises the following steps: 1) performing multiplex PCR amplification on a sample to be detected by using a digestible composite modified primer pair, to obtain a PCR product; 2) performing an enzyme cutting reaction on the PCR product by using a second enzyme mixture containing a primer digestion enzyme, a mismatch correction enzyme and a terminal repair enzyme, including removing the excess primer part on the amplification product by using the digestion enzyme, and removing the non-specific amplification product by using the mismatch correction enzyme; 3) performing a ligation reaction by using a full-length adapter containing an index, to directly obtain a sequencing library or obtain a sequencing library after amplification. The method significantly improves the amplification efficiency and specificity, and has the advantages of simple steps, low cost, short time, wide application value in the fields of genetic disease detection, tumor companion diagnosis and genetic detection and scientific research.

Provided is an internal reference gene for the real-time quantitative PCR (RT-qPCR) of Yersinia enterocolitica, including one of or a combination of some of glnS, nuoB, glmS, gyrB, dnaK, and thrS genes. Further provided is a primer set for amplifying the internal reference gene for the RT-qPCR of Yersinia enterocolitica. Given that stable housekeeping genes of Yersinia enterocolitica remain unclear, internal reference genes with relatively stable expression at different culture temperatures are selected by screening for Yersinia enterocolitica. Specific detection primers and real and reliable data provide a reference basis for selecting housekeeping genes of Yersinia enterocolitica; moreover, the use thereof alone or in combination, as an internal reference gene, improves the data accuracy, stability, and reliability, thereby solving the problem that usually only empirically selecting 16s rRNA as a single internal reference gene in conventional RT-qPCR results in unstable research results.

The present application relates to the technical field of gene detection, in particular to a primer probe combination for detecting Alzheimer's disease, a product and application thereof. The primer probe combination and kit provided by the present application can simultaneously detect Alzheimer's disease gene mutation and methylation, can effectively distinguish Alzheimer's disease patients from non-Alzheimer's disease subjects, realize low-invasive, low-cost, high-sensitivity, high-specificity detection of Alzheimer's disease, the total sensitivity of the blood sample for detecting Alzheimer's disease can reach 88.89%, the specificity of the blood sample for detecting non-Alzheimer's disease can reach 95.74%, the detection probability of false negative results can be reduced, and the detection accuracy is high.

The invention provides a fusion transcript recognition method, device and system based on transcriptome multiple comparison data and a medium, and the core of the method is to construct a set of full-chain analysis framework oriented to multiple mapping reads to realize fusion gene detection. Comprising a pairing read segment preliminary screening module based on a loose mapping strategy, a candidate fusion construction module based on an exon graph structure, a remapping module based on an enhanced reference transcriptome, a transcript abundance estimation module based on a probability generation model, and a fusion scoring module based on fusion site specificity support degree. And a false positive inhibition module based on coverage consistency and biological filtration. According to the method, the dependence of a traditional fusion gene detection method on a unique mapping read is broken through, multiple comparative analysis on the fuzzy mapping read abandoned due to sequence homology is proposed for the first time, and high sensitivity and low false positive rate of the fuzzy mapping read are verified in simulated data and real tumor samples; the method has remarkable technical advancement, clinical applicability and expandability.

The present application relates to the field of gene mutation detection, in particular to a gene detection reagent, kit, detection method and application for predicting susceptibility to myopia, comprising specific detection primers and / or fluorescent probes for detecting rs524952, rs148443109, rs75714645, rs7290586, rs188276693 and rs1420853 gene polymorphism detection sites. The present application also discloses a kit comprising the aforementioned reagent and a detection method. The reagent, kit and detection method of the present application can detect the aforementioned six gene sites respectively, and by determining the genotyping of the six gene sites of the patient, the risk population of myopia can be identified through analysis, and the prevention and control of myopia can be guided.

The utility model relates to gene detection technical field discloses a kind of auxiliary devices for gene detection, including operation box, three first receiving grooves are provided in operation box top, rotating cylinder is rotatably connected in three first receiving grooves inside, two adjusting columns are fixedly connected to the surface of three rotating cylinders symmetrically, adjusting mechanism for adjusting rotating cylinder is equipped on the surface of two adjusting columns, two second receiving grooves are symmetrically provided in three rotating cylinders inside, fixing mechanism for fixing gene tube is equipped in two second receiving grooves inside, constraint block is slidably connected in the bottom of three rotating cylinders, rotating shaft is fixedly connected in the bottom of three constraint blocks, three rotating shafts are rotatably connected in the inner surface of first receiving groove, worm wheel is sleeved on the surface of three rotating shafts, rotating mechanism for rotating rotating cylinder is equipped on the surface of three worm wheels. The utility model can avoid the phenomenon that sample appears multiple shaking by the setting of worm wheel.

This invention relates to the field of gene detection technology and discloses a high-throughput gene sequencing data multivariate quality control system, including a sample information management module, a sequencing data acquisition module, a multivariate quality control analysis module, a result output and traceability module, an equipment collaborative control module, and a data storage module. Each module communicates bidirectionally via a data bus to achieve real-time data transmission and interaction. This invention addresses the dual needs of clinical medical testing and forensic evidence identification, adapting to multiple application scenarios and solving the problem of single-scenario application in existing systems. It can simultaneously meet the quality control requirements for non-invasive prenatal testing, reproductive genetic testing, tumor gene testing, and forensic kinship identification, eliminating the need for multiple quality control systems, reducing equipment costs and operational complexity, and improving laboratory efficiency.

The application belongs to the technical field of gene detection, and particularly relates to a composition for meat adulteration identification, a kit containing the same and application. The application provides a composition for meat adulteration identification, which comprises a first nucleic acid composition, a second nucleic acid composition and a fluorescent probe group. The nucleotide sequence of the first nucleic acid composition is shown as SEQ ID NO:1-6, and the nucleotide sequence of the second nucleic acid composition is shown as SEQ ID NO:8-13. The composition has high sensitivity, high detection efficiency and high specificity, and can be used for meat adulteration identification.

The invention relates to the technical field of gene detection, and provides a fluorescent quantitative PCR (polymerase chain reaction)-based primer probe group for detecting pathogenic microorganisms of root rot of Chinese chives, which comprises forward primers JBLB-F, FPLB-F and CCLB-F, reverse primers JBLB-R, FPLB-R and CCLB-R, and probes JBLB-P, FPLB-P and CCLB-P. The invention also provides a fluorescent quantitative PCR-based leek root rot pathogenic microorganism detection kit, which comprises the primer probe group. Meanwhile, the invention also provides a fluorescent quantitative PCR-based method for detecting pathogenic microorganisms of root rot of Chinese chives. Based on a probe method fluorescent quantitative PCR technology, in combination with high-sensitivity design of a specific primer probe group, accurate detection of low-copy pathogen nucleic acid can be realized, existence and quantity of pathogens can be determined in the early stage of the pathogens of the leek root rot, and early intervention and early warning of the root rot are facilitated.

An Artificial Intelligence (AI) based computational system is used to non-invasively estimate the presence of a range of aneuploidies and mosaicism in an image of embryo prior to implantation. Aneuploidies and mosaicism with similar risks of adverse outcomes are grouped and training images are labelled with their group. Separate AI models are trained for each group using the same training dataset and the separate models are then combined, such as by using an Ensemble or Distillation approach to develop a model that can identify a wide range of aneuploidy and mosaicism risks. The AI model for a group is generated by training multiple models including binary models, hierarchical layered models and a multi-class model. In particular the hierarchical layered models are generated by assigning quality labels to images. At each layer the training set is partitioned in the best quality images and other images. The model at that layer is trained on the best quality images, and the other images are passed down to the next layer and the process repeated (so the remaining images are separated into next best quality images and other images). The final model can then be used to non-invasively identify aneuploidy and mosaicism and associated risk of adverse outcomes from an image of an embryo prior to implantation.

The present application relates to the technical field of gene detection, in particular to a primer probe set, a kit and application thereof. The primer probe set provided by the present application comprises a first primer probe set, a second primer probe set and optionally a third primer probe set. The present application also provides a kit comprising the primer probe set. The primer probe set or the kit provided by the present application can be used for detecting HLA-B*35:01 allele, has high accuracy and specificity, and can quickly and efficiently realize the detection of HLA-B*35:01 allele.

The utility model discloses a sample pretreatment device for gene detection of 10-50g food samples, which belongs to the field of gene detection of food and meat products and comprises a lower platform, and first to sixth food sample pretreatment units are sequentially arranged on the lower platform. Each food sample pretreatment unit comprises a pretreatment container and a rotary cutting unit, the volume of the pretreatment container is 50ml, the rotary cutting unit comprises a rotating shaft and a blade group for rotary cutting, the blade group for rotary cutting is connected to one end of the rotating shaft, the blade group for rotary cutting is located in a sample area in the pretreatment container, and the blade group for rotary cutting is connected to the other end of the rotating shaft. And the other end of the rotating shaft is positioned above the outside of the pretreatment container. According to the utility model, the pretreatment of 10-50g of food samples can be treated in a classified manner, and the problem of cross contamination is avoided.

The invention relates to the technical field of gene detection, in particular to a gene detection object and application of the gene detection object in preparation of products for detecting plasmid DNA residues in induced pluripotent stem cells, the induced pluripotent stem cells are obtained by transduction of somatic cells through four plasmids pCXWB-EBNA1, pCXLE-hOCT4-shp53, pCXLE-hSK2 and pCXLE-hUL, and the gene detection object can be used for detecting the plasmid DNA residues in the induced pluripotent stem cells. A target sequence of the gene detection object comprises a sequence as shown in SEQ ID NO. 7. The gene detection substance provided by the invention can efficiently detect the plasmid residue in the induced pluripotent stem cells, and the corresponding detection method has high specificity and high sensitivity, and can effectively meet the detection requirements of the plasmid residue in the safety quality control link of the induced pluripotent stem cells in the development and production process of cell treatment products.

This invention discloses a primer set, kit, and method for detecting Brucella, belonging to the field of gene detection technology. The primer set comprises two pairs of specific RPA primers: primer pairs B1F1 (SEQ ID No. 3) and B1R1 (SEQ ID No. 6) targeting the B1 sequence, and primer pairs B2F1 (SEQ ID No. 8) and B2R1 (SEQ ID No. 11) targeting the B2 sequence. This invention also provides a kit containing this primer set and a detection method based on RPA-CRISPR-Cas12a. This application combines the high efficiency of RPA amplification with the ultra-high specificity of CRISPR-Cas12a detection, overcoming the shortcomings of traditional methods such as long processing time, low sensitivity, and susceptibility to false positives. It achieves rapid, sensitive, and specific detection of Brucella, and has significant application value in the early screening and monitoring of brucellosis.

This invention relates to the field of biomedical engineering, specifically to a device and method for high-throughput continuous sampling and gene detection of single live cells. The device includes a cell capture layer and a gene detection layer stacked sequentially from top to bottom. The cell capture layer includes a fluid inlet and a fluid outlet, and at least one capture array disposed between the fluid inlet and the fluid outlet. The at least one capture array is connected to the fluid inlet and the fluid outlet respectively via a microfluidic network. Each row of the capture array includes multiple capture units spaced apart and connected along the fluid flow direction. Each capture unit includes a main channel extending along the fluid flow direction and branch channels, the branch channels sharing the same fluid inlet and the same fluid outlet with the main channel, thereby forming a θ-shaped capture unit. The gene detection layer has multiple collection chambers spaced apart, each containing an electrode. A nanomembrane is disposed at the top of each collection chamber, and a gene detection probe is disposed at the bottom.

An apparatus for generating personalized risk assessments for neurodegenerative diseases includes a computing device that receives user data containing genetic and medical information. It processes the data to create genotype identification and gene detection modules, identifying user genotypes and relevant genetic markers. The user's mitochondrial haplogroup is examined to refine the assessment. A risk calculation module employs machine learning to weigh genetic variants against population-based data, calculating a polygenic risk score (PRS). The PRS forms a personalized risk profile, displayed through a visual interface. The disclosed systems offer a comprehensive approach to accurate risk assessment, enabling targeted interventions and informed decision-making in neurodegenerative disease management.

The invention belongs to the technical field of gene detection, and particularly relates to a prostate cancer bone metastasis diagnostic kit based on gene methylation detection and application of the prostate cancer bone metastasis diagnostic kit. The invention proves that the bone metastasis and non-bone metastasis of the prostate cancer can be effectively distinguished by PCR methylation detection or joint detection based on AKR1B1, RASSF2 and other genes, and a new thought is provided for clinical diagnosis and typing of the bone metastasis of the prostate cancer.

The invention is applicable to the technical field of molecular breeding and gene detection technologies, and provides a Haiford cattle 5K low-density SNP chip and application thereof.The chip comprises liquid phase capture probes corresponding to 5001 SNP loci, the SNP loci are evenly distributed on 29 autosomes of Haiford cattle, and the liquid phase capture probes correspond to the 5001 SNP loci. Variety characteristic sites, 63 important economic character related QTL sites and cattle mandibular osteogenesis related causal sites are covered. The length of the liquid-phase capture probe is 120bp, the 5'end is modified by a biotin group, and the requirements that the GC content is 20-80%, the Tm value is 60-80 DEG C and the like are met. The chip is low in detection cost, the site detection rate is 100%, the chip can be used for Haiford cattle variety purity identification, genetic relationship analysis, economic character related molecular marker detection and genetic defect causal site detection, and an effective technical means is provided for variety identification, inbreeding risk assessment and economic character molecule auxiliary selection in the Haiford cattle breeding process.

The utility model discloses a biological sample cracking device, a gene detection kit and gene detection equipment, relates to the field of biological sample treatment, and aims to solve the problem that the application scene of a transverse stirring structure is limited in related technologies. The biological sample cracking device is applied to the gene detection kit which is vertically used, a vertical cracking cavity is formed in the gene detection kit, a sample adding opening communicated with the cracking cavity is formed in the top end of the gene detection kit, and the biological sample cracking device comprises an opening sealing cover, a rotary transmission part and a stirring cutting part. The opening sealing cover is used for being connected with the sample adding opening and sealing the sample adding opening; one end of the rotary transmission part is rotatably connected with the opening sealing cover; the opening sealing cover is provided with a containing hole, and the transmission structure of the rotary transmission part is exposed through the containing hole; the stirring and cutting part is fixedly arranged at the other end of the rotary transmission part and is accommodated in the cracking cavity. The device has the advantages of being wide in application range, simple and compact in structure and capable of improving the cracking effect.