509 results about "Genetic testing" patented technology

The invention discloses a lung cancer gene mutation classification method based on frequency domain multi-scale fusion guidance, and relates to the technical field of medical image processing and gene detection. According to the MFHA mechanism provided by the invention, the pathological image is decoupled into low-frequency global and high-frequency detail sub-bands through wavelet transform, and extraction of key high-frequency features such as cell nucleus morphology and local texture is enhanced by combining multi-scale convolution and up-sampling guided by high-frequency information; the problems of insufficient feature detail mining and low feature fusion efficiency in a traditional pathological image analysis method are solved; key features are screened and focused through a channel, frequency domain-space feature deep fusion is realized through up-sampling, robust representation is constructed by combining space attention with cosine similarity and multi-dimensional statistical features, a frequency domain analysis-space focusing collaborative optimization mechanism is formed, information redundancy caused by simple feature splicing is avoided, and the robustness of the system is improved. And the classification stability of the model in a complex pathological scene is improved.

The invention discloses a primer group, a kit and a method for detecting brucella, and belongs to the technical field of gene detection. The primer group comprises two pairs of specific RPA (recombinase polymerase amplification) primers: a primer pair B1F1 (SEQ ID No.3) and a primer pair B1R1 (SEQ ID No.6) targeting a B1 sequence, and a primer pair B2F1 (SEQ ID No.8) and a primer pair B2R1 (SEQ ID No.11) targeting a B2 sequence. The invention also provides a kit containing the primer group, and a detection method based on the RPA-CRISPR-Cas12a. The invention also provides a kit containing the primer group and a detection method based on the RPA-CRISPR-Cas12a. According to the application, the high efficiency of RPA amplification is combined with the ultrahigh specificity of CRISPR-Cas12a detection, the defects of long time consumption, low sensitivity and easy generation of false positive in the traditional method are overcome, rapid, sensitive and specific detection of Brucella is realized, and the application has important application value in early screening and monitoring of Brucella.

The invention belongs to the technical field of gene detection, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker, a primer pair, a kit, a detection method and application of a g.64930 site of a PPARG (Peroxisome Peroxisome Activated Receptor Gene) gene of Anhui local variety cattle. The nucleotide sequences of the molecular marker are as shown in SEQ ID NO.1 and SEQ ID NO.2, when the basic group at the 118th site of the 5'end of the molecular marker is G, the meat color brightness of beef is higher than that when the basic group at the site is T, and the muscle pH is lower than that when the basic group at the site is T. The molecular marker can be used as a candidate molecular marker for the quality character of the Anhui local variety cattle, is applied to population breeding of the Anhui local variety cattle, and is combined with production requirements for molecular-assisted breeding, so that a theoretical basis is conveniently provided for variety resource development and breeding of the Anhui local variety cattle.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The invention belongs to the technical field of gene detection and gene molecular breeding, and particularly relates to a meat duck whole genome molecular probe combination based on molecular phenotype screening, a 50K gene chip and application thereof. The molecular probe combination and the gene chip of the marker site combination for meat duck whole genome breeding simultaneously cover 7 representative meat duck varieties and 71 economic characters, have richer polymorphism and higher pertinence in meat duck groups, and are lower in cost and higher in speed compared with high-throughput sequencing detection; the breeding chip is designed according to the growth, feed efficiency, slaughtering, breeding, egg quality and various molecular phenotypes of the meat ducks, and compared with a high-throughput sequencing technology, the breeding chip is higher in seed selection accuracy, has higher breeding value, can be widely applied to breeding genotype detection of the meat ducks, and can be used for detecting the breeding genotypes of the meat ducks. And the method has creative significance in the aspect of meat duck genome selective breeding.

The application belongs to the field of biochemical detection, and specifically discloses a new use of a GPR18 and other gene detection agent combination and a depression detection reagent. The application of the GPR18 expression level detection agent in the preparation of a major depressive disorder detection product. The expression amount of GPR18, PDK4, NRG1 and EPHB2 in the application actually has high diagnostic efficiency, and can be used as an independent candidate diagnostic biomarker. The accuracy of each as a screening indicator reaches 0.6 to 0.8, reaching the standard of a medium-strength screening indicator, and the accuracy of the combined expression amount indicator as a screening indicator reaches 0.779.

The invention relates to a detection kit for combined methylmalonemia, which is a cb1C type kit and comprises a specific primer pair and a probe combination for detecting at least one mutation site of MMACHC, the mutation site is selected from c.609Ggt; a is c.567 dupT, c.658660delAAG, c.482Ggt, and c.482Ggt; a, c.1Agt; g, c, 80Agt; g, c, 217Cgt; 315Cgt, T, c.315Cgt; g and c.394 Cgt; t). The MMACHC gene hotspot mutation region detection kit based on the real-time fluorescent quantitative PCR technology can detect multiple mutation sites on the MMACHC gene at the same time, detection of three genotypes of wild type, homozygous mutant type and heterozygous mutant type on each mutation site is completed, the gene detection requirements of clinical combined type MMA are effectively met, the detection time is shortened, and the detection efficiency is improved. The mutation site coverage rate of CblC defective methylmalonic acidmia combined with homocysteinemia on the MMACHC gene is increased to 93.28%, mutation hot spots of most people are covered, and various types of mutation conditions in hot spot mutation areas can be rapidly, accurately and sensitively detected.

The invention belongs to the technical field of gene detection, and particularly relates to application of a CFTR gene mutation combination, an amplification reagent and a product in preparation of a CF risk assessment product. The CFTR gene mutation combination consists of an intron region mutation combination and an exon region mutation combination, based on the CFTR gene mutation combination, the invention further develops an amplification reagent and a CFTR gene mutation detection product, the amplification reagent and the CFTR gene mutation detection product comprise a primer combination for multiplex PCR amplification of the CFTR gene mutation combination, and the primer combination has the characteristics of high accuracy, high specificity and high sensitivity, can accurately detect related gene mutation, and provides a reliable basis for risk assessment and diagnosis of CF. When the amplification reagent and the CFTR gene mutation detection product are combined with sweat chlorine detection for use, the diagnosis rate of CF can be remarkably improved, and the amplification reagent has important application value in clinical diagnosis of CF and is expected to provide more powerful support for early diagnosis and treatment of CF patients.

The invention relates to a primer group, a kit and a method for detecting an ATXN3 gene (CAG) n trinucleotide repetition number, and belongs to the technical field of gene detection. The technical problem to be solved by the invention is to provide the primer group for detecting the n trinucleotide repetition number of the ATXN3 gene (CAG). The primer group comprises a first primer pair and a second primer pair, a forward primer sequence of the first primer pair is as shown in SEQ ID NO. 1, and a reverse primer sequence of the first primer pair is as shown in SEQ ID NO. 2; a forward primer sequence of the second primer pair is as shown in SEQ ID NO. 3, and a reverse primer sequence of the second primer pair is as shown in SEQ ID NO. 4. Through the design of the two pairs of primers, complementary verification is achieved, the detection result is comprehensive and reliable, the technical defects of first-generation sequencing and second-generation sequencing on long-fragment and high-GC-content repetitive sequence detection are overcome, the detection cost is low, the detection period is short, and the primer pair is suitable for clinical detection service.

The invention relates to the technical field of gene detection, and discloses a mycobacterium tuberculosis drug resistance gene detection data analysis system which comprises a data import module, a signal correction module, a feature extraction module, a multi-stage interpretation module, a result fusion module and a report generation module. The system performs baseline drift correction, abnormal point detection and nonlinear normalization on a fluorescence intensity value output by a detector, extracts multi-dimensional features such as peak intensity, a mutation ratio, a consistency coefficient and an in-batch difference index, and constructs a dynamic threshold function to realize wild type and mutation type layered judgment. A drug resistance risk conclusion is generated through drug resistance characteristic matrix mapping, a standardized detection report is output, and intelligent analysis of a detection result and automatic report generation are achieved. Through multi-stage signal correction, multi-dimensional feature extraction and dynamic threshold interpretation, intelligent analysis and standardized report output of a mycobacterium tuberculosis drug resistance gene detection result are realized, and data judgment accuracy and clinical application efficiency are improved.

The invention discloses a lutjanus erythropterus breeding auxiliary method and system based on multi-character collaborative selection, and the method comprises the steps: carrying out phenotype data collection and gene detection, and constructing a standardized phenotype matrix and a genotype matrix; performing genetic parameter estimation and breeding value prediction based on the standardized phenotype matrix and the genotype matrix to obtain breeding value prediction information; the method comprises the following steps: analyzing an association relationship between characters of lutjanus rubripes to be bred, constructing a character association network, and carrying out multi-character dynamic weight configuration to obtain a dynamic weight set; performing genome selection index calculation and parent priority analysis according to the breeding value prediction information and the dynamic weight set to generate a parent selection priority list; and generating a candidate parent pool through the parent selection priority list, performing hybridization combination analysis, performing genetic gain prediction on the generated hybridization combination, and generating a hybridization auxiliary scheme. The genetic gain accuracy and balance of breeding of the lutjanus erythropterus are improved, the limitation of traditional experience selection and matching is broken through, and the breeding efficiency is improved.

The invention relates to the technical field of data screening, in particular to a tumor gene detection report auxiliary analysis system. The data acquisition module is used for acquiring medical record feature vectors and habit vectors of the medical record data; the feature analysis module is used for obtaining disease similar feature values by analyzing the difference relationship between the medical record feature vectors, establishing classification in combination with the consistency of the habit vectors, and calculating feature parameters of the historical medical record data in each category; the habit influence analysis module is used for analyzing the difference condition between the dimensions of the habit vector and the difference condition between the characteristic parameters of the medical record data, and constructing an influence curve of each dimension; and the medical record screening and auxiliary analysis module is used for combining the difference condition between the habit vectors of the to-be-detected medical record data and the historical medical record data, the influence curve and the disease similar characteristic value, and is used for screening adaptive medical records, and the adaptive medical records can more effectively carry out tumor gene detection auxiliary analysis on the to-be-detected medical record data at the moment.

The invention relates to a pathogenic gene identification method and system based on multi-agent debate and a medium. The method comprises the following steps: acquiring gene detection data and clinical phenotype data; the data agent processes the acquired data, and calls a gene variation pathogenicity analysis operator and a molecular genetic large model to obtain first pathogenic gene information; the knowledge agent calls a molecular genetic large model to obtain second pathogenic gene information based on the first pathogenic gene information; the knowledge and data agents sequentially speak and debate pathogenicity of candidate pathogenic genes in the first or second pathogenic gene information on the basis of sorting results in the pathogenic gene information output by the knowledge and data agents; after each round of debate is finished, the debate agent judges whether the sorting results of the data and the knowledge agent on the candidate pathogenic genes are consistent or not, if the sorting results are consistent or the number of debate rounds reaches a threshold value, debate is finished, the debate agent conducts reasoning and outputs a result, and if not, the next round of debate is started. Compared with the prior art, the method has the advantages of no dependence on large-scale training data, high interpretability and the like.

The invention discloses a joint detection kit for genetic risk genes and medication genes of Alzheimer's disease and a multivariable risk assessment model. The kit comprises a detection reagent for detecting 25 mutation sites of 15 genes, wherein the 25 mutation sites of the 15 genes comprise 17 mutation sites of 10 risk genes and 10 mutation sites of 6 medication genes. Amplification primer pairs and single-base extension primers of each site are designed for 25 mutation sites of 15 genes, multiple PCR amplification and single-base extension reactions are carried out, and the genotype of each site of a product is analyzed by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. A multivariable risk assessment model for the Alzheimer's disease is constructed by taking a genetic risk score GRS, age, gender and plasma p-tau217 concentration of a risk gene mutation site as markers, one-stop detection of'early screening and medication guidance 'can be realized in combination with a medication gene detection result, and the application prospect and demand are broad.

The invention relates to the technical field of technical gene detection, in particular to a recombinase and recognition site screening method and system based on structure classification prediction.The method comprises the steps that to-be-matched recombinase is obtained for structure resolution prediction.The functional domain of the to-be-matched recombinase is obtained; searching in a first database according to the functional domain, searching candidate recombinase of which the similarity meets a preset condition, and screening to obtain optimal recombinase; determining an interaction region of the current large serine recombinase and the target DNA sequence from a second database, and sequentially obtaining interaction amino acid sequences corresponding to the preferable recombinase; and matching with the amino acid characteristic sequence of the current large serine recombinase in the second database to obtain the target large serine recombinase with the highest similarity in the preferred recombinase and the corresponding recognition site sequence. According to the method, the structure of the large protein is subjected to structure comparison mining and splitting, and recognition site prediction is performed on interaction region amino acid characteristics, so that the research and development period is shortened, and the research and development cost is saved.

The application belongs to the technical field of gene detection, and particularly relates to a biological sample nucleic acid rapid extraction method and kit based on a magnetic bead method. The method comprises the following steps in sequence: S1, liquefying a biological sample by using a liquefying solution; the liquefying treatment is to add 1-4 times the volume of the liquefying solution to the biological sample, shake and mix, and then place at room temperature for 15-30 minutes; the liquefying solution comprises guanidine salt, potassium salt and / or sodium salt, Tris-HCl, EDTA and isopropyl alcohol; S2, lysing the biological sample; adding a lysing solution and magnetic beads to the system obtained in S1, and heating at 90-100 DEG C for 3-20 minutes to lyse the biological sample; S3, cleaning the biological sample; and S4, eluting the nucleic acid. The method does not need centrifugal separation in the operation process, and can realize automatic and efficient extraction of the nucleic acid.

The invention belongs to the technical field of gene detection, and particularly relates to a primer group for detecting areca-nut addiction viruses and a method for detecting the areca-nut addiction viruses. The primer group comprises APV1-F3-1, APV1-FIP-1, APV1-BIP-1 and APV1-B3-1, and the primer group comprises APV1-F3-1, APV1-FIP-1, APV1-BIP-1 and APV1-B3-1; the nucleotide sequence of the APV1-F3-1 is as shown in SEQ ID No. 1, the nucleotide sequence of the APV1-FIP-1 is as shown in SEQ ID No. 2, the nucleotide sequence of the APV1-BIP-1 is as shown in SEQ ID No. 3, and the nucleotide sequence of the APV1-B3-1 is as shown in SEQ ID No. 4. The invention further discloses a kit for detecting the APV1-F3-1, the APV1-FIP-1, the APV1-FIP-1, the APV1-FIP-1, the APV1-BIP-1, the APV1-BIP-1 and the APV1-B3-1. Based on the primer group, an LAMP-OSD probe detection system and an RT-LAMP-Cas12a integrated detection system are established, and rapid detection of the areca catechu addiction virus (APV1) can be achieved.

The invention discloses a postoperative recurrence monitoring system and method for bile liquid biopsy, and relates to the technical field of medical detection, and the method comprises the following steps: obtaining medication data, bile liquid data and gene data of a target object; preprocessing the medication data and the bile liquid data to generate a sampling interference index sequence, and further performing weighted fusion on the gene data by the sampling interference index sequence to obtain a mutation load index of each gene detection time point; and within the time sequence range of the gene data, performing sliding window analysis on the sampling interference index sequence to generate an abnormal chain marker sequence. According to the method, the time sequence mismatch degree is accurately quantified by constructing a time difference matrix, and a weight distribution mechanism is dynamically adjusted in combination with medication volatility, so that the generation process of the sampling interference index can adaptively reflect the influence of medication mode change on the sampling quality; the problem of interference evaluation deviation caused by mismatching of medication data and bile sampling time is effectively solved.

To provide a gene testing method and a gene testing kit for analyzing a variable region of a TCR or a BCR in single-cell analysis.SOLUTION: A method including a step of capturing mRNA eluted from a single cell using an RT probe immobilized on a solid-phase surface and including a common sequence, a cell identification barcode, a first molecular identification barcode, and an oligo(dT) sequence, a step of performing a reverse transcription reaction in a reverse transcription reaction solution containing a TSO using the mRNA as a template to generate cDNA having a sequence added to a 3' end, a step of degrading the mRNA, a step in which a primer for inserting a second molecular identification barcode binds to a constant region adjacent to a variable region of a TCR or a BCR and includes the second molecular identification barcode, a step of ligating cDNA extended from the primer with a ligase, a step of amplifying a fragment including the second molecular identification barcode and the variable region of the TCR or the BCR using a primer, a step of performing sequence analysis, and a step of associating sequences.SELECTED DRAWING: Figure 2

The present invention relates to a ctDNA-based solid tumor MRD detection method, detection system, and computer-readable medium, and belongs to the technical field of tumor gene detection. The present invention proposes a method for qualitatively characterizing plasma sample MRD based on tissue baseline mutation sites during dynamic plasma testing. This method calculates the background error rate of single-base mutations, simulates mutation readings based on this error rate, and compares them with the actual observed mutation readings. The number of times the simulated mutation readings are greater than the actual observed mutation readings is statistically analyzed. The p-value of the sample ctDNA positive is calculated based on multiple simulation results at the mutation site.

The invention discloses a method and equipment for automatically generating a gene detection report. The method comprises the following steps: acquiring gene detection result data; forming cue word input data of a pre-trained report generation model based on the gene detection result data; and based on the cue word input data, outputting a target interpretation report through the report generation model.

The invention relates to the field of gene detection, and particularly discloses a gene detection device and method applying Beidou satellite positioning, and the device comprises a sample collection and preprocessing module which is used for obtaining a body fluid or tissue biological sample of a user, and carrying out cell lysis, DNA / RNA extraction and purification operation on the sample; the gene sequencing analysis module is connected with the sample collecting and preprocessing module and is used for performing gene sequencing on the purified nucleic acid sample and identifying gene variation information related to tumors; the high-precision positioning capability of the Beidou system is utilized to obtain the environmental parameters of the geographic position of the user in real time, then the genetic variation data and the regional environmental carcinogenic factors are subjected to weighted fusion analysis through the risk assessment algorithm, and finally a highly personalized detection report is generated. In this way, the detection result can truly reflect the specific influence of the external environment on the individual health, and the accuracy and practicability of the report are remarkably improved.

The utility model relates to the field of medical detection, provides a sample storage and treatment device for gene detection, and aims to solve the problems of inconvenience in storage, easiness in omission and risk of pollution of DNA (deoxyribonucleic acid) samples during treatment in the prior art, and the sample storage and treatment device comprises a shell, a puncher, a punching die and a storage positioning mechanism, the punching die is arranged on the shell; the storing and positioning mechanism is detachably connected to the punching die and used for storing and positioning the dried blood spot collection cards during punching. The storage positioning mechanism can store a plurality of dried blood spot collection cards after sampling and can also perform positioning; when the blood spot needs to be punched and cut out, the storage positioning mechanism is directly installed on the shell, and the puncher is used for punching. And through the matched design of the storage positioning mechanism and the punching die, the dried blood spot collection cards do not need to be sequentially taken out for punching during sample punching treatment, pollution to dried blood spot collection card samples for Rh blood group gene detection is effectively reduced, and more convenience is achieved. The method is especially suitable for large-scale Rh blood group gene screening.

The application relates to the technical field of gene detection, and particularly discloses a kit for human autosomal miniSTR typing and application thereof. The kit comprises 10 pairs of STR primer groups for amplifying 10 miniSTR loci, and the 10 miniSTR loci are D3S1358, D21S11, D18S51, D7S820, D15S131, D15S12, D15S146, D16S539, D5S818 and TPOX. The kit can successfully perform STR typing on highly degraded DNA samples, and can be used as a supplement of traditional STR reagents.

The invention relates to the technical field of molecular biology, in particular to a reference gene for Chinese olive functional gene expression analysis and application thereof. According to the invention, the reference gene RPN2B which is most stably expressed in fruits of different varieties (lines) of Chinese olives in different development and maturation periods is screened out, and in addition, two reference genes NIFS1 and RPS16 which are only second to the RPN2B are also screened out, so that the method can be used for expression analysis of nutritional quality or other functional genes of the fruits of the Chinese olives of different varieties (lines) in different development and maturation periods; the specificity, the stability and the accuracy of functional gene expression analysis data are remarkably improved, and powerful support is provided for Chinese olive functional gene expression analysis. The invention also provides a special detection primer for detecting the reference gene of the Chinese olive fruit, and the detection primer can quantify the expression quantity and function analysis of the auxiliary gene through fluorescence in the Chinese olive function research, and has high application value.

The invention discloses an accurate report automatic analysis and generation system based on gene detection data, and belongs to the technical field of bioinformatics and artificial intelligence. The system comprises a variation intelligent labeling module, a heterogeneous knowledge graph reasoning module, a scene adaptive report generation module and an intelligent quality control and feedback module; a three-layer heterogeneous knowledge graph containing gene-disease association, drug-gene interaction and clinical guide decision is constructed, variation-disease association reasoning is performed by adopting a relational graph attention network, scene adaptive generation of report content is realized in combination with a semantic slot filling mechanism, and the four modules form a closed-loop collaborative system through deep coupling. Gene detection data can be automatically converted into a clinical diagnosis report, and the report generation time is shortened from 48 hours to 30 minutes or less.

The invention provides an abnormal glycolipid metabolism risk dynamic assessment system based on SNP and gene analysis and application of the system in neuropsychiatric drug therapy, and belongs to the technical field of biological medicine. The system disclosed by the invention contains the primer probe group for detecting the metabolic influence of the antipsychotic drug, so that specific amplification detection of a specific SNP site can be realized, clinical guidance is targeted, and abnormal glucose and lipid metabolism and significant change of body weight are avoided. According to the method, glucose and lipid metabolism and effect target gene detection products are adopted for detection, a detection result is evaluated and interpreted through a glucose and lipid metabolism abnormal risk dynamic evaluation algorithm, retrospective investigation and actual case observation are combined, the detection result is good in sensitivity, high in accuracy and high in practicability, sampling is convenient, the evaluation algorithm is professional and reasonable, and the method is suitable for popularization and application. Results are easy to understand and easy to popularize and use.

The utility model provides a cell centrifugal separation device for gene detection, include: shell main part and cooperation seal's sealing cover, be provided with the inner bag in the shell main part, the inner bag bottom cooperation is provided with speed reducer, speed reducer output end extends to the inner bag, speed reducer output end top cooperation is provided with centrifugal separation main part, by this, the utility model has the advantages that, the device simple structure, stability is good, is favorable to cell centrifugal separation, the shell main part has the temperature, the rotating speed and the time required for cell centrifugal separation, and the speed motor is the most power source, realizes synchronous locating disc rotation through transmission, the test tube positioning frame that carries cell test tube moves synchronously with synchronous locating disc synchronously, and the centrifugal angle is adjusted adaptively with the rotating speed difference, reaches the stable cell centrifugal separation effect.

The application discloses a kind of methods, systems, equipment and media for analyzing EML4-ALK fusion gene type, belong to the technical field of fusion gene detection.The method comprises obtaining RNA sequencing data of a sample to be tested;According to the primer combination coverage area, obtain the primer coverage area sequence file;The RNA sequencing data is compared with the primer coverage area sequence file for the second time;The number of sequencing data that can be aligned to the exon sequence of two genes at the same time is counted.Using the method and system of the application, unknown type of fusion gene can be accurately detected, which is more conducive to the development of downstream research on the related mechanism and function of fusion gene.

The invention relates to a full-length sequence amplification primer, an amplification method and a three-generation sequencing method of an HLA-I antigen gene, and belongs to the field of gene detection. The HLA-I antigen gene is subjected to PCR (Polymerase Chain Reaction) amplification by using 8 pairs of characteristic amplification primers. And each gene can be subjected to high-yield specific amplification on a full-length sequence containing complete intron and exon regions only through one round of amplification and enrichment, and 7.8 Kb long-fragment genes can be captured once at most. The experimental deviation caused by simultaneous amplification of a single gene by using a plurality of pairs of segmented primers and the indirect error caused by gene splicing are effectively avoided. The method comprises the following steps: carrying out quality inspection and purification on amplicon PCR (Polymerase Chain Reaction) products, mixing samples, constructing an HLA-I type antigen gene library, carrying out accurate and complete sequencing on the full-length sequence of the HLA-I type antigen gene by adopting a three-generation PacBio Sequel II platform, and ensuring that the Hifi reads base accuracy can reach 99% or above. The reading of HLA-I type antigen gene full-length sequence variation information and the haplotype analysis at a high resolution level can be realized.