The invention discloses a gene detection method, a gene detection kit and gene detection equipment. The gene detection method comprises the following steps of arranging a plurality of separation cavities in the kit, blocking the every two adjacent separation cavities by a plunger, and adding a cracking liquid, a cleaning liquid and a reaction liquid into each separation cavity; when a sample is detected, pushing each plunger, aligning a plunger hole of each plunger with the corresponding separation cavity, and further conducting each separation cavity; utilizing an electromagnetic control type to control magnetic beads in the kit to drive the to-be-tested sample to sequentially pass through the separation cavities, and then sequentially cracking, cleaning and reacting; finally, performing optical detection on the gene in the reaction liquid from the exterior.