77 results about "Short Tandem Repeat Profile" patented technology

This invention provides compositions and methods for genetic testing of an organism and for correlating the results of the genetic testing with a unique marker that unambiguously identifies the organism. The markers may be internal markers, such as for example single nucleotide polymorphisms (SNPs), short tandem repeats (STRs), or other sites within a genomic locus. Alternatively, the markers may be external, such that they are separately added to the genetic sample before testing.

The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample.

The invention relates to a compound amplification system for simultaneously analyzing a plurality of STR (short tandem repeat) loca, which is characterized by being used for carrying out compound amplification on 20 locas: Amelogenin, vWA, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D19S433, D6S1043, D12S391, D8S1179, D3S1358, CSF1PO, Penta D, Penta E, TH01 and TPOX. The invention also relates to a method and a kit for simultaneously analyzing DNA (deoxyribonucleic acid) samples and other applications of the loca.

The invention relates to the technical field of biology, and relates to a human X-chromosome composite amplification system composed of 20 short serially-connected repetitive sequences and applications thereof. The composite amplification system comprises 20 pairs of primers and is capable of amplifying 19 STR sites: DXS6795, DXS6803, DXS6807, DXS9907, DXS7423, GATA172D05, DXS101, DXS9902, DXS7133, DXS9810, GATA31E08, DXS6800, DXS981, DXS10162, DXS6809, GATA165B12, DXS10079, DXS10135, and HPRTB, and one sex recognition site: Amelogenin. Five-color fluorescence labeling is adopted by the system, the size of amplification products is in a range of 85 to 450 bp, the operation is simple, and the individual recognition performance is high. The sites provided by the provided composite amplification system are highly compatible to the conventional commonly-used X-STR kits, moreover, sites with high polymorphism information are provided, and the provided composite amplification system can be applied to paternity identification related with X-chromosome, individual recognition, and auxiliary detection of X-chromosome sex-linked hereditary diseases.

The invention relates to a composite amplification system of 23 short tandem repeat sequences and a kit, is used for detecting heredity mark gene of polymorphism in a mankind genome, and belongs to the biology technical field. The invention relates to a scheme that a plurality of short tandem repeat sequences are simultaneously amplified in a PCR system; a specific gene locus comprises 22 short tandem repeat sequences with high level heredity polymorphism and 1 gender determination locus; primers are respectively designed and a fluorophore is marked; the system has very high individual recognition rate and non-father elimination rate; the system and kit can be used for legal medical individual recognition, paternity tests, and colony genetics analysis, is high in accuracy, and good in sensitivity.

The invention provides a detection device and a detection system. The detection device comprises an acquisition unit, an analysis unit, a check unit and an output unit, wherein the acquisition unit is used for acquiring DNA sample data which is generated by sampling through a DNA sequencer; the analysis unit is used for performing short tandem repeat sequence analysis on the DNA sample data and generating analysis result data; the check unit is used for checking the DNA sample data, verifying whether the DNA sample data has errors, and generating check result data; and the output unit is used for outputting the analysis result data and corresponding check result data. The detection device replaces the manual check process so as to efficiently and accurately finish the identification check work for the identification result and omit the process for analyzing and outputting the short tandem repeat sequence for the DNA sample data with poor check result, and reduces the cost.

The invention supplies a fluorescence labeling verification system by compounding and expanding 14 short series repeating sequence locus to take personal identification and paternity test. The specific compounding method controls the expanding product in the range of 400 basic groups, and only labeling three fluorescence of the compounding expanding of the 14 locus would be realized. The invention takes a deep research to the gene characteristic of the 14 locus and the allele in five minorities in China, and supplies the compounding expanding verification system. The agent box suited for Chinese throng is researched.

The invention relates to a composite amplification method and kit for STR loci. The composite amplification method and kit are used for amplifying 22 STR loci and one sex locus, wherein the 22 STR loci are composed of vWA, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D19S433, D6S1043, D12S391, D8S1179, D3S1358, D1S1656, CSF1PO, Penta D, Penta E, TH01, TPOX, D10S1248 and DYS391, and the sex locus is Amelogenin. Amplimers used in the composite amplification method and kit comprise sequences as shown in SEQ ID No. 1 to 46. The composite amplification method and kit provided by the invention can acquire information about the 22 STR loci with good polymorphism and high individual discrimination power and about the one sex locus through simultaneous amplification at a time; and a composite amplification system composed of the loci has good balance, high sensitivity and high specificity, is accurate in typing results and can totally meet demands of judicial expertise.

The invention provides a kit for realizing multiple amplification of 21 short tandem repeats by adopting degenerate primers, and belongs to a multiple amplification system for simultaneously analyzing a plurality of STR gene loci. 21 gene loci are amplified in a multiple amplification system simultaneously, and are divided into the following four groups: the first group: D19S433, TH01, D21S11, D18S51 and Penta E; the second group: D3S1358, D13S317, D7S820, D5S818, CSF1PO and Penta D; the third group: Amelogenin, vWA, D8S1179, TPOX, D6S1043 and D1S1656; and the fourth group: D16S539, D12S391, D2S1338 and FGA. Specific primers are respectively designed on flanks of a gene loci repetitive sequence, so that the balance of the peaks of the fragments inside the gene loci groups achieves 40% or above, the four gene loci groups are subjected to multiple amplification, and the concentration of the primer of each gene locus is regulated, so that the integral balance of the peaks of the gene loci achieves 30% or above. With the kit, a balanced and stable multiple amplification result is easily obtained, so that the kit belongs to a multiple amplification system with low cost and high efficiency.

The invention discloses a method for non-invasive preimplantation hereditary detection of embryos, and belongs to the technical field of biological detection. The method comprises the following steps:carrying out whole genome amplification on a blastocyst culture solution sample by using a kit, carrying out short tandem repeat sequence analysis on an amplification product and DNA samples of parents to detect maternal pollution, carrying out library preparation and next-generation sequencing detection on the amplification product to determine whether the number of chromosomes is abnormal or not; and optimizing a pre-amplification mixed solution and an amplification mixed solution by the provided kit. According to the method provided by the invention, the blastocyst culture solution can besubjected to parent source pollution detection, so that whether the chromosome aneuploidy detection result of the culture solution is accurate and reliable or not is judged. The invention provides a detection method for judging whether granular cells are completely removed or not, and the inhibition effect of components in the culture solution on amplification is effectively avoided through optimization of the kit, so that amplification uniformity is good, and the single cell amplification yield is high. The detection method is simple, the result is accurate, and data quality is improved.

The application discloses an accurate human DNA typing method, a reagent and application. According to the accurate human DNA typing method, a short tandem repeat sequence, polynucleotide polymorphicsites covering a whole genome, a mitochondrial DNA hypervariable region I, a mitochondrial DNA hypervariable region II and an Amel enamel gene are detected simultaneously to obtain accurate typing andbase sequences of all the sites, thereby realizing accurate typing of human DNAs. The disclosed method having high resolution ratio is capable of carrying out efficient and accurate individual recognition and has the extremely high individual recognition capacity for difficult detection materials and highly-degraded detection materials; the non-parent exclusion rate is larger than 99.999999% in paternity identification and the accuracy is high; the compatibility is high, the existing individual recognition detection kit can be covered, and the method can be used for analyzing race groups in different regions or countries by combining human genome data. Besides, the method disclosed by the invention is relatively high in adaptability of detection materials and the human DNA extracted by various experimental methods can be detected.

The invention provides a multiplex amplification kit of 18 short tandem repeats (STR), relating to a multiplex amplification system which simultaneously analyzes a plurality of STR loca. The multiplex amplification kit is characterized by carrying out multiplex amplification on the following 18 loca: Amelogenin, D6S477, GATA198B05, D15S659, D8S1332, D3S3045, D17S1290, D14S608, D2S441, D18S535, D13S325, D10S1435, D11S2368, D1S1656, D7S3048, D10S1248, D19S253 and D3S1358. The invention also relates to a method and kit for simultaneously analyzing DNA (deoxyribonucleic acid) samples and applications thereof.

An identification method and its regent box for recognizing the unit DNA by the allelic ladder of short series-wound repeating serial locus have been opened the invention. The process is: Screening the SRT locus, designing the common reading things for synthesizing special PCR, then to mark the leading things, respectively. Then to extract the DNA of the human, going on the PCR reaction, detecting the part by electrophoresis and purifying the part. Last to connect the product of PCR to the carrier, then getting the clone of the different genes, making the every allele PCR reaction by using the leading thing of fluorescent number, mixing the PCR products of allele for STR locus then adding to the stabilizer. So we can get the allelic ladder. The ladder is the regent box to recognize the DNA. The invention needn't to collect the different gene blood again and again. And the box cost little. It is easy to make, also have good stability.

The invention provides a preparation method of a placenta source matrix mesenchymal stem cell. The preparation method comprises the following steps: (1) separating the placenta source matrix mesenchymal stem cell; (2) cultivating the placenta source matrix mesenchymal stem cell, wherein a trypLE<TM> enzyme solution is utilized to process a placenta tissue in two steps in the step (1), and a serum-free medium is utilized in the step (2). By adopting the method provided by the invention, the placenta source matrix mesenchymal stem cell is a complete matrix source by short tandem repeat sequence (STR) atlas analysis, and is qualified by chromosome karyotype examination, pathogenic microorganism examination of bacteria, funguses and viruses, cell purity identification and cell biology function identification. Therefore, the placenta source matrix mesenchymal stem cell obtained by the method provided by the invention does not contain foreign protein, can meet the requirements of clinical use, is especially suitable for use by mothers, and also provides a technical support for building an excellent placenta source matrix mesenchymal stem cell bank.

The invention discloses a STR gene data analysis method. On the basis of C + + language, short tandem repeat (STR) gene sequencing data in DNA is subjected to positioning full-automatic analysis, thedata processing efficiency is integrally improved, a gene region associated with specific characters is finally obtained, and a rich positioning result display chart is provided. The algorithm supports analysis of multi-color fluorescence channels, supports the kit commonly used in the market at present, suports an original data file format output by a common sequencer, can automatically identifyand remove miscellaneous peaks generated in inspection, and can automatically calibrate peak pattern dislocation generated in the sequencing process. According to the invention, full-automatic data analysis of a multi-color fluorescence color channel is carried out on a DNA gene short tandem sequence STR in DNA identification and detection by a forensic medicine. The situation that DNA sequencingonly depends on foreign software analysis is broken through, a process is established for developing domestic sequencers and domestic reagents in China, and a domestication development process in thefield of forensic identification and detection of DNA in China is accelerated.

The invention relates to a method for fast detecting common chromosome trisome by quantitative fluorescence PCR, which comprises the following steps: sample collection and processing, DNA extraction, primer design and synthesis, PCR amplified reaction of a first system and a second system, PCR product detection, PCR amplified reaction of a third system and PCR product detection. The invention also provides an accurate and reliable reagent kit for fast detecting the common chromosome trisome by the quantitative fluorescence PCR. The invention is integrated with the sensitivity and the accuracy of the quantitative fluorescence PCR and the extensive and uniform distribution, the polymorphism and the high heterozygosity of a tandem repeat sequence and can be used for detecting the number of common chromosomal disorders of prenatal samples, such as peripheral blood, amniotic fluids, cord blood, tomenta, and the like.

The invention relates to a composite amplification system and a detection kit of a mouse short tandem repeat and belongs to the technical field of biotechnology. The invention relates to mouse short tandem repeats (D2Mit395.1, D4Mit170.1, D5Mit201.1, D7Mit40, D11Mit4, D15Mit16 and D17Mit51.1) and a sex determination site (Jarid1) for simultaneously amplifying mice in a PCR (polymerase chain reaction) system, carrying out composite amplification and capillary electrophoresis detection on mouse DNA (deoxyribonucleic acid). The system can be used for genotyping of a mouse cell, each site adopts fluorescence-labeled primers, so that an obtain product is provided with a fluorescence label, and the detection can be carried out on an apparatus such as a genetic analyzer, so that the obtain segment length is more exact, and higher in repeatability. The system is simple to operate and convenient for large-scale popularization, the whole process has automation equipment, and the whole detectiontime only needs about 6 hours.

The invention relates to the technical field of biology, and relates to a composite amplification system of 28 short tandem repeats, a kit and an application thereof. The composite amplification system comprises 28 pairs of primers, and can be used for amplifying 28 sites at the same time; and the invention also discloses a primer sequence aiming at the 28 sites. By means of a research of geneticdiversity of a STR locus, 28 sites which contain 22 new CODIS sites, 4 sites with high discrimination power (D4S2366, D6S1043, D11S2368, D14S608) and one gender recognition site Amel, and one Y chromosome insertion-deletion site Y-indel are selected. The sites have higher discrimination power, excluding probability of paternity and polymorphism information content, and other characteristics, so that paternity test and discrimination power system effectiveness can reach a higher level.

The invention is directed to methods for detecting and quantifying nucleic acid contamination in a tissue sample of an individual containing T cells and / or B cells, which is used for generating a sequence-based clonotype profile. In one aspect, the invention is implemented by measuring the presence and / or level of an endogenous or exogenous nucleic acid tag by which nucleic acid from an intended individual can be distinguished from that of unintended individuals. Endogenous tags include genetic identity markers, such as short tandem repeats, rare clonotypes or the like, and exogenous tags include sequence tags employed to determine clonotype sequences from sequence reads.

The invention belongs to the gene engineering technical field, and discloses a primer combination for detecting a short tandem repetitive sequence, a kit containing the primer combination and a method for detecting the short tandem repetitive sequence. Gene loci having high China people genetic polymorphism are selected and are more suitable for China people; the number of the selected tandem repetitive units is four, five or six, the slip peak ratio can be effectively reduced, and the accuracy of test results is higher; a fluorescent labeling composite amplification system is used for amplification, and amplified products can be subjected to electrophoresis in a same capillary, to achieve detection analysis of all the gene loci at the same time; a fluorescent label with mature technology is used for fluorescent labeling, and thus the cost is low; the detection method has good specificity and can be used for qualitative analysis such as individual identification, paternity identification, population genetic studies and the like, and can also be used for qualitative analysis of detection of the chimerism rate after hematopoietic stem cell transplantation.

The invention relates to a multiplex amplification system for analyzing a plurality of STR (short tandem repeat) gene loci simultaneously. The system is characterized in that 25 gene loci are subjected to multiplex amplification and include: Amelogenin, vWA, D10S1248, D2S441, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D22S1045, D1S1656, D19S433, D6S1043, D12S391, D8S1179, D3S1358, CSF1PO, Penta D, Penta E, TH01, TPOX and DYS391. The invention further relates to a method and a kit for analyzing DNA (deoxyribonucleic acid) samples and applications of the method and the kit.

The invention provides a preparation method of a universal short tandem repeat (STR) allele ladder. The preparation method comprises the following steps of (1) designing and synthesizing a primer anda plasmid according to an STR locus; (2) using the primer to perform a PCR amplification reaction by using the plasmid as an amplification formwork; and (3) performing purification on PCR amplification products, and performing appropriate dilution so as to obtain the STR allele ladder. According to the preparation method disclosed by the invention, only one plasmid needs to be synthesized, the PCRreaction needs to be performed only once, and after the PCR products are subjected to purification and dilution, the allele ladder of the corresponding STR locus can be obtained, so that the preparation technological process of the allele ladder of the STR locus is greatly simplified, and labor cost,, time cost and economic cost for preparation are saved.

The invention provides a new STR (short tandem repeat, Short tandern repeat,) preparation method of allele ladder (Allele ladder, AL), which comprises extracting DNA from human nucleated cells, and synthesizing corresponding to these STRs The primers of the site, select the DNA of individuals with different alleles, use PCR technology to specifically amplify the corresponding amplification products, use HPLC to purify and separate the amplified products, and perform PCR amplification→purification→PCR for multiple cycles Amplification→purification, and finally the purified products with different alleles at each site are mixed in equal proportions to form each STRAL. The present invention also provides a typing kit with the allele ladder prepared by this method. The preparation method and typing kit described in the invention can be used in aspects such as individual identification, paternity identification and gene diagnosis in the fields of forensic science, anthropology, genetics and diseases.

The invention discloses a method for preparing a human gene STR (short tandem repeat) sequence allelic ladder by using the site-directed mutagenesis technology. The typing of the known STR locus is disclosed; in the invention, homologous arm primers are designed according to different types of the STR locus; different types of a locus are obtained by a site-directed mutagenesis method, and then the obtained different locus types are cloned into plasmids; and therefore, the work of early sample screening is saved, and the preparation efficiency of an STR ladder is improved.

The invention relates to a multiplex amplification system and a detection kit for a short tandem repeat sequence of a mouse, and belongs to the technical field of biological detection. According to the multiplex amplification system and the detection kit for the short tandem repeat sequence of the mouse, nine STR (Short Tandem Repeat) loci, which have high sensitivity and amplification specificity, of the mouse are selected; the STR loci are amplified once; the loci amplified by the multiplex amplification system are some STR loci which can be used for identifying a cell line of the mouse; when the cell line of the mouse is identified, the multiplex amplification system and the detection kit have the advantages of being quick, being simple to operate and accurate in result, being convenient for large-scale popularization, and the like. By using a method, along with the successful development of the kit, the batch production can be realized; a working condition can be modeled; automatic equipment exists in all processes; human operation experience is not depended on any more; the automation can be realized; a required time is short; a whole detection time needs approximate 6 hours.

The invention relates to the field of in-vitro diagnosis, and more specifically relates to a universal probe for simultaneously detecting multiple sites of a genome, and a primer design method and anapplication kit thereof. The universal probe adopts a short tandem repeat sequence as a skeleton sequence, and solves the problems that the number of the probes in a reaction system in which the copynumber concentration is amplified is too large, and non-specific amplification is easily formed and the synthesis cost is high, and the concentration of the chromosome copy number can be proportionally amplified by designing multiple pairs of the primers on both sides of the universal probe position of the single copy gene fragment of the chromosome to-be-tested, which is especially suitable for the detection of certain very low concentration samples (such as whether the non-invasive detection of chromosome copy number generates variation or not).

The invention relates to the field of information analysis of sequencing data, and particularly provides a method for detecting and typing the repetition number of a short tandem repeat sequence based on next-generation sequencing. According to the detection method, two flanking sequences can be compared at the same time across a middle repeated region, and mismatching, insertion and deletion of the flanking sequences are considered at the same time; the typing method is an STR typing method for performing dynamic threshold setting by combining repetition number difference based on detection of an effective peak value, and can be applied to detection of repetition numbers of different next-generation sequencing platforms.

The present invention provides a composite amplification system, for analyzing multiple STR loci at the same time, where it amplifies the 20 loci: Amelogenin, vWA, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D19S433, D6S1043, D12S391, D8S1179, D3S1358, CSF1PO, Penta D, Penta E, TH01 and TPOX. Also provided are the methods and kits for analyzing DNA sample at the same time, as well as the uses thereof.

The invention discloses a new short nucleotide tandem repeat sequence locus and application thereof. The invention provides the short tandem repeat locus G2S0002, the sequence of which is shown as SEQ ID NO.:1, and the short tandem repeat locus G2S0002 can be used for preparation of: (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity test or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and / or (e) kits for detecting whether white blood cells in a bone marrow transplantation receptor is replaced by donor cells. The short tandem repeat locus G2S0002 has a high degree of distinction, and can effectively analyze the genetic relationship.