452 results about "Infectious Disorder" patented technology

The invention discloses a preparation method of a protein halo-coated silicon-coated gold nanorod and a PCR amplification method and kit based on the protein halo-coated silicon-coated gold nanorod, and belongs to the technical field of PCR detection. The photo-thermal nano material is composed of an AuNR coated SiO2 core-shell structure and an external protein halo layer, the protein halo layer avoids inhibition of AuNR on a PCR reaction system, meanwhile, adsorption of AuNR on biological small molecules is effectively reduced, non-specific amplification is reduced, and normal amplification of PCR reaction is guaranteed; and the protein halo layer is used as a thermal insulation layer to optimize heat exchange and maintain the temperature uniformity of the reaction liquid. When the kit is applied to a photo-thermal PCR detection reagent, DNA of a pathogen to be detected can be efficiently and stably amplified and detected within 10 minutes, the kit has huge application potential in the fields of early screening of infectious diseases, food safety detection and the like, collaborative optimization of photo-thermal conversion efficiency, biocompatibility, detection sensitivity and operation convenience is achieved, and the kit has a wide application prospect. And an efficient solution is provided for prevention and control of infectious diseases, food safety monitoring and biomedical research.

The invention discloses a respiratory infectious disease multi-pathogen nucleic acid detection kit based on a fluorescent PCR detection technology and application of the respiratory infectious disease multi-pathogen nucleic acid detection kit. The invention provides two detection kits aiming at 20 and 6 respiratory tract detection, the two kits can specifically detect respiratory tract pathogens, the detection sensitivity is high, the whole detection system is short in time consumption and simple and convenient in use process, and detection results of 20 pathogenic microorganisms can be reported within 40 min at the shortest; the whole set of detection kit and the detection method are high in sensitivity and good in specificity; the normal-temperature storage and transportation of the reagent can be realized.

The invention relates to the technical field of nucleic acid on-site rapid visual detection, in particular to a one-pot rapid nucleic acid detection system based on an SfaTnpB combined isothermal amplification technology and application of the one-pot rapid nucleic acid detection system. According to the invention, an RAA isothermal amplification technology and an LAMP isothermal amplification technology are respectively combined with SfaTnpB nuclease, and finally, an RAA-SfaTnpB one-pot method system and an LAMP-SfaTnpB one-pot method system are successfully established. The one-pot system established by the invention has the advantages of high sensitivity, high specificity, fast reaction, strong accuracy and no need of complex equipment, the detection result can be visually interpreted by naked eyes, the system has high convenience and practicability, the adaptability and practicability of the system are further improved, and the rapid detection requirements in various scenes can be flexibly met. The system has a wide application prospect in the fields of rapid pathogen detection, genotype detection, early diagnosis of infectious diseases and the like.

The invention provides a solid-phase agglutination detection test paper card and a detection method thereof. A reaction area of the solid-phase agglutination detection test paper card comprises a reaction film, the reaction film is coated with captured protein and can enrich to-be-detected antibodies in a to-be-detected sample, and the captured protein is combined with the to-be-detected antibodies and then subjected to agglutination reaction with granular antigens in a detection reagent to form an agglutination compound. And the agglutination compound is intercepted in the reaction film for color development. The granular antigen is a cell carrying a specific antigen or a conjugate formed by coupling the specific antigen and an inert carrier, and the granular antigen can be colored or has a luminescence property in various modes. The solid-phase agglutination detection test paper card provided by the invention has the advantages of simple detection steps, short detection time, high sensitivity, accurate detection result, strong specificity and the like, can be used for detecting various diseases and monitoring antibody level, and also has important significance in the fields of infectious disease prevention and control, medical diagnosis and the like.

The disclosure provides compositions, methods of treatment, and methods of making and using compositions to deliver a nucleic acid to a subject. Methods of using these compositions as a vaccine for treatment of an infectious disease are also provided.

The present disclosure relates to compositions and methods, including Trim / inhibitors that find use in the treatment of disease, such as therapies for cancer, including cancers that are resistant to anti-checkpoint agents, infectious diseases and inflammatory diseases.

The invention provides a bacillus thuringiensis strain, a fermentation culture method and application of the bacillus thuringiensis strain. The strain is preserved in the China Center for Type Culture Collection on September 28, 2011, the preservation address is No. 299 on eight road, Wuchang District, Wuhan City, Hubei Province, the preservation number is CCTCC No: M 2011334, and the preservation date is September 28, 2011. The strain cultured by the fermentation method has efficient, specific and stable insecticidal activity on culex, aedes and other mosquitoes, has the advantages of low preparation cost, environmental friendliness and the like, can be developed into a biological insecticide for preventing and killing mosquitoes and preventing and controlling malaria, dengue fever, chikungunya and other mosquito-borne infectious diseases, and meets the requirements of green and safe prevention and control in China.

The invention provides a solid-phase agglutination detection test paper card directly marked by antigens and a detection method thereof. A reaction area of the solid-phase agglutination detection test paper card comprises a reaction film, the reaction film is coated with captured protein and can enrich to-be-detected antibodies in a to-be-detected sample, and the captured protein is combined with the to-be-detected antibodies and then subjected to agglutination reaction with granular antigens in a detection reagent to form an agglutination compound. And the agglutination compound is intercepted in the reaction film for color development. The granular antigen is a conjugate formed by coupling a specific antigen and an inert carrier, the specific antigen is not a protein, and the specific antigen is specifically combined with an antibody to be detected. The solid-phase agglutination detection test paper card provided by the invention has the advantages of simple detection steps, short detection time, high sensitivity, accurate detection result, strong specificity and the like, can be used for detecting various diseases and monitoring antibody level, and also has important significance in the fields of infectious disease prevention and control, medical diagnosis and the like.

The invention relates to the technical field of infectious disease detection, and discloses a new respiratory infectious disease early warning method, which comprises the following steps: S1, specimen collection and data collection: collecting biological samples of new respiratory infectious disease virus infected individuals and other respiratory infectious disease virus infected individuals, the biological sample comprises a blood sample and a swab sample; meanwhile, basic information of the individuals is collected, wherein the basic information comprises gender, age and respiratory symptom duration; s2, quantitative detection: the biological sample is subjected to quantitative detection by adopting reagents of various respiratory infectious disease viruses, the detection content comprises antigens and antibodies, and the antibodies comprise an I gM antibody, an I gG antibody and an I gA antibody; according to the invention, basic information of individuals and antigen-antibody detection reagent resources of various respiratory infectious disease viruses are deeply integrated, so that the recognition capability of new respiratory infectious disease virus infected individuals is effectively improved.

The invention discloses a rapid detection and intelligent early warning system for port concerned infectious diseases, and relates to the field of customs quarantine, and the system comprises a target selection unit, a construction unit, a modification unit, an amplification unit, a purification unit, a determination unit and a detection unit. According to the invention, through systematic improvement in the aspects of multi-primer design and modification, amplification system optimization, product purification, multi-dimensional scoring decision and the like, many defects in the aspects of multi-pathogen detection, dimer interference suppression, detection efficiency and sensitivity improvement in the prior art are overcome; rapid, efficient and intelligent screening and real-time early warning of port concerned infectious diseases are realized.

The invention belongs to the field of poultry infectious diseases, and particularly relates to a riemerella anatipestifer phage mutant strain and a method for quickly mutating riemerella anatipestifer phage. Escherichia coli bacteriophage, riemerella anatipestifer bacteriophage and corresponding host bacteria are mixed, the infection complex number is made to be larger than 100, after culture, supernate is taken and then mixed with the host bacteria for culture, a bacteriophage mutant strain is separated from a secondary culture solution, the phage spectrum of the bacteriophage mutant strain is detected, and the mutant strain with the broadened phage spectrum is screened. At present, no report for widening the bacteriophage spectrum of the riemerella anatipestifer based on similar principles is found, and the research can fill the blank in the related research field.

A method for treatment or prophylaxis of a disease in a subject involving administering to the subject a vaccine composition including non-immunomodulating, engineered, plant-derived extracellular vesicles (EVs), is provided. The Evs are loaded with an exogenous nucleic acid molecule encoding a protein antigen. The disease is an infectious disease or cancer. A method for the preparation of the vaccine composition, which makes use of one or more polycationic substances and one or more sugar molecules is also provided.

T cell responses are exquisitely antigen-specific and directed against peptide epitopes displayed by human leukocyte antigen (HLA) on the surface of presenting cells. In particular, class II HLA (HLA-II) is remarkably polymorphic, which allows for presentation of diverse peptide antigens to T cells, but also forms the basis for genetic associations with diverse immunopathologies across the spectrum of infectious disease and autoimmunity. Here, Applicants employ monoallelic immunopeptidomics to retrieve over 200,000 unique peptides presented by 41 HLA-II heterodimers covering major alleles across diverse ancestries. Applicants leveraged this expansive dataset to develop computational models that predict peptide antigens based on HLA-II binding properties and infer informative features of the protein antigens from which these peptides derive. Combining both peptide and (contextual) protein features, Applicants develop Context Aware Predictor of T cell Antigens (CAPTAn) to discover novel T cell epitopes from prokaryotes in the human microbiome and the viral pandemic pathogen SARS-COV-2.

Antibody molecules that specifically bind to LAG-3 are disclosed. The anti-LAG-3 antibody molecules can be used to treat, prevent and / or diagnose cancerous or infectious disorders.

The present invention relates to specific binding molecules which bind to the HLA-E restricted peptide RLPAKAPLL (SEQ ID NO: 1) derived from Mycobacterium tuberculosis enoyl-ACP reductase. Said specific binding molecules may comprise CDR sequences embedded within a framework sequence. The CDRs and framework sequences may correspond to a T cell receptor (TCR) variable domain and may further comprise non-natural mutations relative to a native TCR variable domain. The specific binding molecules of the invention are particularly suitable for use as novel immunotherapeutic reagents for the treatment of infectious disease.

Disclosed herein are compositions that include antigen-encoding nucleic acid sequences and / or antigen peptides. Also disclosed are nucleotides, cells, and methods associated with the compositions including their use as vaccines, including vectors and methods for a heterologous prime / boost vaccination strategy.

Provided are novel antibodies and antigen-binding fragments thereof that bind interleukin-18 receptor beta (IL-18Rβ), along with conjugates thereof, nucleic acids encoding the same, compositions comprising the same, and methods of producing and using the same, including in the treatment of various diseases and conditions, such as inflammatory bowel disease, and other immune-mediated diseases, autoimmune diseases, inflammatory diseases, cancers, and infectious diseases.

The invention discloses a sheep infectious disease number zero individual tracing method and system based on a group interaction network, particularly relates to the technical field of agricultural informatization, and is used for solving the problem of number zero individual misjudgment caused by neglecting group dynamic behaviors in an existing static network model. The method comprises the following steps: dividing a continuous time window to construct a time sequence interaction network by acquiring dynamic contact data and high-risk medium use data of a sheep flock; virtual edges are added to individuals with the use interval smaller than the pathogen survival time of the same high-risk medium; calculating a node behavior mode mutation degree based on a medium using interval variance, and analyzing propagation fluctuation in combination with an input / output propagation flow ratio standard deviation; identifying nodes of which behavior mutation exceeds a threshold value, propagation fluctuation continuously exceeds the threshold value and output propagation flow suddenly drops as an abnormal propagation source; and extracting a first window associated sub-network, and judging the node which has the highest behavior mutation degree and uses a high-risk medium in the sub-network as a zero individual, thereby realizing accurate tracing of the sheep infectious disease source and providing a basis for accurate prevention and control of a farm.

The invention discloses a mosquito distribution prediction method and system, a storage medium and equipment, and relates to the technical field of data processing, and is used for predicting the mosquito distribution state, and the method comprises the steps: synchronously collecting environment data and mosquito population data, carrying out the time-space alignment, building a nonlinear correlation model through combining a bidirectional LSTM model and transfer learning, and carrying out the prediction of the mosquito distribution state. Complex nonlinear correlation between environmental factors and mosquito population dynamics can be accurately captured; mosquito distribution of different time scales is predicted by using a graph neural network and a Transform architecture, and accurate modeling of a multi-scale space-time interaction rule of mosquito distribution is realized. The prediction result is calibrated through a deviation correction mechanism, the temporal-spatial resolution and accuracy of mosquito distribution prediction are remarkably improved, and reliable data support is provided for accurate prevention and control of insect-borne infectious diseases.

The disclosure provides compositions and methods for treating and vaccinating invertebrates and invertebrate populations from diseases.

The invention relates to the technical field of image or video recognition or understanding, and discloses an areca yellows early warning method based on regular economic forest multi-dimensional symptom analysis, in the method, the unique morphology of areca and various morphological changes which are invisible under trees but visible in remote sensing during the period that areca suffers from yellows are fully utilized, and the areca yellows early warning effect is achieved. And the multiple symptoms are combined to form a multi-symptom matching degree, so that the misrecognition problem can be eliminated in an auxiliary manner when specific symptoms exist. When the specific symptoms do not exist, whether the disease of the current areca-nut forest belongs to the infectious disease or not is indirectly judged according to the distribution characteristics of the multi-symptom matching degree in time and space by utilizing the distribution characteristics and the disease course development rule when the areca-nut is cultivated as an economic arbor, and if yes, the disease of the current areca-nut forest belongs to the infectious disease; and considering that the areca yellows occupy a large proportion in the areca infectious diseases, the pathogen detection cannot be infeasible due to entrainment of a large number of irrelevant diseased plant samples when the pathogen detection is carried out at the moment. The above points are combined to realize early warning of areca yellows.

Bartonellosis is an emerging group of infectious diseases caused by bacteria belonging to the genus Bartonella, which includes at least 22 named species of bacteria transmitted primarily by carriers (i.e., vectors) including fleas, pubic lice, nits, sand flies, ticks, and potentially mites and spiders. In some aspects, the present disclosure provides antigen-specific amino acid sequences of Bartonella.

The present disclosure provides for a synthetic immunogenic protein for use as an immuno-modulatory agent to enhance mammalian immune reactions towards conjugated protein or peptide containing antigens that are otherwise poorly immunogenic, including but not limited to self-antigens. The chimeric immunogenic proteins of the present disclosure can be used in the treatment of many illnesses, including but not limited to cancers, infectious disease, autoimmune disease, allergies and any clinical indication involving or affected by the immune response of a mammalian host.

The invention belongs to the field of biological quarantine, and particularly relates to a method for separating and identifying pigeon-derived liver escherichia coli. Escherichia coli separation and identification are carried out on disease samples from Guangdong, Guangxi, Guizhou, Hainan and the like, and the epidemicity of the disease samples is determined; collecting livers of acute dead young meat pigeons, and separating strains; carrying out gram staining, physiological and biochemical identification and molecular identification on the separated strain; the growth and reproduction cycle and hemolysis characteristics of the strain are detected, 76 Escherichia coli infection occurs in 113 disease samples, and the infection rate is 67%. According to the method, laboratory diagnosis of infectious diseases of the meat pigeons is systematically completed through sample collection and separation, culture and identification of strains, it is determined that the young meat pigeons are infected by escherichia coli, and a foundation is laid for subsequent antibiotic-free green breeding of the meat pigeons resisting escherichia coli infection.

Provided herein are immunoconjugate molecules containing a TNF-α polypeptide and a masking moiety capable of inhibiting and activating the TNF-α activity under suitable conditions. Also provided herein are methods for producing the immunoconjugate molecules. Finally, provided herein are the therapeutic uses of the immunoconjugate molecules due to their modulating effects on the immune system for treating diseases such as cancer and other chronic infectious diseases.

The present invention relates to formula (I): [Formula 1] A compound of TIFF2026514449000425.tif27170, or its tautomers, stereoisomers, salts, solvates, or N-oxides, wherein A 1 ~A 6 X, Y, R 7a , R 7b , R 8a , and R 8b The claims are as defined. The present invention further relates to pharmaceutical compositions comprising the compound and a pharmaceutically acceptable carrier, and their uses as agents, in particular as agents having METTL3 inhibitory activity, and advantageously for use in the treatment or prevention of cancer, or autoimmune diseases, neurological diseases, infectious diseases, or inflammatory diseases.

The present invention relates to a cyclic peptide, a conjugate comprising said cyclic peptide and a lipopeptide building block, a bundle of said conjugates, a synthetic virus-like particle comprising at least one bundle of conjugates and pharmaceutical compositions comprising the same. The present invention further relates to said cyclic peptide, said conjugate said bundle of conjugates, said synthetic virus-like particle and said pharmaceutical compositions for use as a medicament, preferably for use in a method for preventing of an infectious disease or reducing the risk of an infectious disease, more preferably for use in a method for preventing or reducing the risk of an infectious disease associated with or caused by a respiratory syncytial virus.

The present disclosure provides adoptive T cell therapies that have improved architectures for targeting antigens and recruiting multimeric immune signaling complexes for treating, preventing, or ameliorating at least one symptom of a cancer, infectious disease, autoimmune disease, inflammatory disease, and immunodeficiency, or condition associated therewith.

The present invention relates to certain compounds that function as inhibitors of DHX9 activity (RNA Helicase A / RHA or Nuclear DNA Helicase II / NDH II). The present invention also relates to processes for the preparation of these compounds, to pharmaceutical compositions 5 comprising them, and to their use in the treatment of proliferative disorders, such as cancer, autoimmune and infectious diseases, as well as other diseases or conditions in which DHX9 activity is implicated.

The invention relates to the technical field of protective clothing, in particular to multifunctional infectious disease protective clothing which comprises a protective clothing body, two cuffs of the protective clothing body are each sleeved with a fixedly-connected wrapping sleeve, an elastic sleeve is fixedly connected to an opening of each wrapping sleeve, a tightening mechanism is installed on the outer side of each elastic sleeve, and each tightening mechanism comprises a fixing base; a groove is formed in the fixing base, an inserting rod is fixedly connected into the groove, and a first pull rope is fixedly connected to one side of the fixing base. The covering sleeve structures are additionally arranged at the cuffs of the protective clothing, the elastic sleeves provided with the tightening devices are ingeniously integrated on the covering sleeves, in practical application, a worker can wrap the outer sides of the cuffs of the protective clothing with the gloves, the edges of the gloves are tightly attached and wrapped through the flexibility and ductility of the elastic sleeves, and the protective clothing is convenient to use. And then the elastic sleeve is effectively shrunk by operating the tightening mechanism, so that an excellent sealing effect is achieved, and the risk that liquid pollutants intrude into the protective clothing from the cuffs of the protective clothing is effectively prevented.