39 results about "Serine proteinases" patented technology

Fish derived serine proteinases including trypsins and chymotrypsin derived from cod such as Atlantic cod is used for treating and / or preventing a variety of diseases and disorders such as inflammatory diseases, infectious diseases caused by viruses, bacteria and fungal species and diseases where a receptor binding mechanism is involved in the pathogenesis. Pharmaceutical and cosmetic compositions comprising the proteinases are described.

A non-surgical method for preventing or reducing the rate of the progression of non-proliferative diabetic retinopathy to the proliferative form of diabetic retinopathy comprising intravitreally administering to a patient suffering from non-proliferative diabetic retinopathy an effective amount of serine proteinase enzyme sufficient to create, without surgery, a posterior vitreal detachment to prevent or reduce the progression of proliferative diabetic retinopathy in said patient. Also disclosed is a non-surgical method of treating ocular conditions such as retinal ischemia, retinal inflammation, retinal edema tractional retinal detachment, tractional retinopathy, vitreous hemorrhage and tractional maculopathy by intravitreally administering to a patient suffering from one or more of these conditions with an effective amount of a serine proteinase enzyme to reduce or treat that particular ocular condition. Plasmin, microplasmin and miniplasmin are preferred serine proteinase enzymes and plasmin is the most preferred.

The present invention provides a new type balloon dilation catheter which includes ballon and medication material coated on stent. Said medication material comes from one or two and more than two mixtures of heparin sodium, fiber degrading enzyme, serine proteinase, batroxobin, aspirin, genistein, hirudin and its recombined product, colchicine, sirolimus, biolimus, zotarolimus, tracrolimus, pimecrolimus, simvastatin, atorvastatin, pravastatin, ciclosporin, Anti-CD34, dexamethasone, bleomycin, plicamycin, daunomycin, mitomycin C, actinomycin D, taxol, celastrol, methopterin, 5-fluorouracil, cytarabine and 6-purinethol. The balloon is made of macromolecule nylon material, and the stimulation to blood vessel is far lower than the stent with metal structure.

A non-surgical method for preventing or reducing the rate of the progression of non-proliferative diabetic retinopathy to the proliferative form of diabetic retinopathy comprising intravitreally administering to a patient suffering from non-proliferative diabetic retinopathy an effective amount of serine proteinase enzyme sufficient to create, without surgery, a posterior vitreal detachment to prevent or reduce the progression of proliferative diabetic retinopathy in said patient. Also disclosed is a non-surgical method of treating ocular conditions such as retinal ischemia, retinal inflammation, retinal edema tractional retinal detachment, tractional retinopathy, vitreous hemorrhage and tractional maculopathy by intravitreally administering to a patient suffering from one or more of these conditions with an effective amount of a serine proteinase enzyme to reduce or treat that particular ocular condition. Plasmin, microplasmin and miniplasmin are preferred serine proteinase enzymes and plasmin is the most preferred.

The invneiton relates to expression, purification and crystallization of urokinase catalytic domain mutant, relating to the field of medicine, biotechnique, and structural biology. And the invention provides a techical method of expression, purification and crystallization of urokinase catalytic domain mutant in Pichia pastoris. And the cultured urokinase catalytic domain mutant has crystallographic space group R3, and unit cell parameters: a=b=120.48, c=42.45, alpha=beta=90.0deg, and gama=120.0 deg. And this protein has serine proteinase activity, appliable to high flux screening of urokinase inhibitor; and its high resolution crystal provides a research and development platform for design and development of urokinase inhibitor.

The invention relates to a Bacillus licheniformis engineering bacterium capable of efficiently secreting nattokinase, and belongs to the technical fields of enzyme engineering and microbes. The method adopts a molecular biology technology to screen and replace signal peptides with important effects in an exogenous protein transhipment process, and the signal peptides are replaced by signal peptides from levanase SacC in Bacillus subtillis 168 from signal peptides from extracellular serine proteinase Vpr in Bacillus licheniformis WX-02 on the basis of nattokinase producing Bacillus licheniformis engineering bacterium BL10 (pP43SNT) preserved in a laboratory in advance in order to afresh construct the nattokinase producing Bacillus licheniformis engineering bacterium BL10 (pP43SacCNK). The bacterium disclosed in the invention can substantially improve the nattokinase secreting level under liquid fermentation conditions, and the maximum enzyme activity can reach 33.83 FU / mL. The bacterium is preserved in China Center for Type Culture Collection on June 16, 2014 with the preservation number of CCTCC NO: M2014253.

Fish derived serine proteinases including trypsins and chymotrypsin derived from cod such as Atlantic cod is used for treating and / or preventing a variety of diseases and disorders such as inflammatory diseases, infectious diseases caused by viruses, bacteria and fungal species and diseases where a receptor binding mechanism is involved in the pathogenesis. Pharmaceutical and cosmetic compositions comprising the proteinases are described.

The present invention relates to one kind of recombinant serine proteinase. The recombinant serine proteinase contains one kind of fusion protein comprising serine proteinase from fungus and chitin binding domain from insect chitinase fused together and contains one gene coding serine protein from fungus and one gene coding chitin binding domain from insect chitinase with separated nucleotide sequences. The recombinant serine proteinase of the present invention may be enriched in insect body wall to raise insect body wall degrading speed and raise pesticidal fungal virulence.

The invention provides a kit for detecting Aeromonas hydrophila by FQ-PCR (fluorescent quantitative polymerase chain reaction). In a reagent packed in the kit, a sense primer and a reverse primer and TaqMan probe have one or two groups of A group and B group. Compared with the prior art, as the based factors only exist in the virulent Aeromonas hydrophila strain, the method can accurately detect whether the virulent Aeromonas hydrophila strain exists in the sample, thereby improving the early warning accuracy of the disease. Simultaneously, if the detection result shows that the template contains the hemolysin gene (hlyA) or serine proteinase gene (ahpA) by using the A group or B group reagent, this indicates that the disease has certain pathogenicity; and if the detection result shows that the template contains the hemolysin gene (hlyA) and serine proteinase gene (ahpA) by using the two groups of reagents, this indicates that the disease has high pathogenicity.

Production of natto kinase and its use are disclosed. The process is carried out by taking sugar, soy-bean and starch as raw materials, using hay bacillus high-yield thrombolytics kinase strain, deep liquid fermenting, adjusting metabolism by complex system, membrane separating, and freeze-drying to obtain the final product. It belongs to serine proteinase and can be used to melt thrombus, inhibit platelet coagulation, prevent hypertension and arteriosclerosis, adjust blood fat and keep blood flow unblocked, and it can be developed as infusion granule, tablet, capsule and oral liquid.

By adopting gene recombination technology to implement recombination of vascular endothelial growth factor (VEGF)2-5 exon and gran zyme B (GraB) gene and make VEGF2-5 and GraB be connected and formedinto fusion gene by means of a part of elastic connecon, and utilizing the specific combination of VEGF2-5 and VEGFR to guide GraB and make it target-act on the nescent vascular endothelial cell so as to attain the goal of target-resisting angiogenesis. Said invention uses prokaryocyte secretion type expression system to express recombinant VEGF-Gra fusion protein, and uses immobilized metal ion affinity chromatography and gel filtration method to purity the fusion protein, and uses GraB serine proteinase activity test experiment and chick embryo allantoic membrane angiogenesis anther membrane inhibition experiment to determine the bio-activity of recombinant VEGF-GraB fusion protein.

The invention discloses a multifunctional liquid laundry detergent. The multifunctional liquid laundry detergent is composed of, by weight, the following raw materials: 30-50 parts of alkyl polyglucoside, 10-15 parts of coconut oil fatty acid diethanolamide, 4-9 parts of hydrolyzed wheat protein, 5-10 parts of serine proteinase, 5-10 parts of sapindus saponin, 2-6 parts of a plant extract liquid,1-3 parts of an olive fruit extract, 0.2-0.5 part of citric acid, 0.5-1 part of essence, and 50-70 parts of water. The liquid laundry detergent of the invention does not contain phosphorus, the essence, pigment, a fluorescent agent, and a bleaching agent, is easy to wash, is high in biodegradability, can effectively remove dirt on clothes, does not hurt clothing fibers, makes clothing soft and smooth, is antistatic, and at the same time, has strong bactericidal and bacteriostatic effects and no stimulus and damage to the skin.

The invention provides agkistrodon acutus hemocoagulase-B which is high-activity hemocoagulase separated from agkistrodon acutus venom. The hemocoagulase is single-stranded glycoprotein comprising 236 amino acids, and has an amino acid sequence shown as SEQ ID No. (sequence identification number) 1. The strand comprises 6 pairs of disulfide bonds; molecular weight of SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is approximately 35kD; the molecular weight of the hemocoagulase after being deglycosylated is 26116.7Da; and an isoelectric point (pI) is 6.0. A hemocoagulase molecule is modified by heterozygotic polysaccharides at an Asn<77, 100, 229> (asparagine) site. The hemocoagulase is serine proteinase. The invention also provides a separation and purification method of the hemocoagulase, which comprises the steps that undissolved substances are removed through pretreating; then two times of anion-exchange column chromatography and one time of Sephdex-G75 molecular sieve chromatography are conducted; an active elution peak is acquired; dialysis and lyophilization are conducted; and the high-purity agkistrodon acutus venom hemocoagulase is obtained. The specific activity of the agkistrodon acutus venom hemocoagulase is not less than 2000U / mg; the analysis purity of HPLC (high performance liquid chromatography) can reach above 95%; and the yield is 0.25-0.30% calculated according to the weight of an agkistrodon acutus venom raw material.

The invention provides a composition containing serine proteinase, a reversible inhibitor of the serine proteinase and a stabilizer M with formula I. The invention also provides an application of the composition as a medicament, other applications and a method of using various characters of the composition.

The invention discloses a special long-acting slow-release fertilizer for fig, which is composed of the following components in parts by weight: 6-10 parts of benzyl octyl adipate, 5-9 parts of octyl benzyl adipate, 4-8 parts of nepenthe, 6-11 parts of galingale, 10-30 parts of Fructus Forsythiae, 3-7 parts of radix adenophorae, 3-8 parts of tetraphosphorus trisulfide, 1-5 parts of tyrosine, 3-8 parts of asparamide, 3-5 parts of glutamine, 3-7 parts of serine proteinase, 6-8 parts of ammonium benzoate, 8-14 parts of ammonium permanganate, 1-3 parts of proteinase aspartate, 1-4 parts of papain and 3-4 parts of slow-release agent. The special long-acting slow-release fertilizer for fig can supply nutritional ingredients required by fig for a long time, and enhances the soil fertility due to the addition of the microbial inoculant and organic fertilizer.

The present invention relates to a method for preparing medicine with the action capable of inhibiting proteniase and its detection method. Said method includes making the aqueous solution containing alpha 1-proteinase inhibitor undergo the pocesses of virus inactivation treatment, ion exchange resin adsorption and elution; at the same time using the reaction of serine proteinase and alpha 1-proteinase to result in photoabsorption change of correspondent wavelength so as to quantitatively determine the activity of alpha 1-proteinase inhibitor. The invented preparation method can effectively implement high yield, can ensure high purity and high activity of product, at the same time its process is simple, cost is low, and its detection method is simple and easy to implement, and its sensitivity is high.

The invention provides a serine proteinase which comes from cordyceps sinensis hirsutella sinensis and participates in hydrolysis of macro-molecule protein for generating micromolecule protein, an encoding gene and application of the two. The amino acid sequence of the serine proteinase is shown as SEQ ID No. 1, and the sequence of the encoding gene is shown as SEQ ID No. 2. Detailed research is performed on the serine proteinase at a principle level in the process of the serine proteinase infecting hepialidae larva, and the serine proteinase which comes from the corbrin-capsule-producing strain cordyceps sinensis hirsutella sinensis and participates in the infection mechanism, and the encoding gene thereof are provided. A cloned DNA of the provided nucleotide sequence can be transferred into an engineering bacterium through a transduction, transformation or conjugal transferring method. By adjusting expression of the proteolytic enzyme gene, a host is endowed with property of highly expressing serine proteinase, an effective approach is provided for expanding biological application of serine proteinase, and important application prospect is provided.

The invention relates to the field of gene cloning, and particularly relates to a freshwater shrimp KSPI (Kazal-type Serine Proteinase Inhibitor) gene, and an amplification method and amplification primer group thereof. By utilizing the primer group provided by the invention, a full-length cDNA sequence of the KSPI gene is cloned from a freshwater shrimp spermary tissue, thereby laying a foundation for the research on the function of the freshwater shrimp KSPI gene, and simultaneously accumulating background data for the research on the procreation of shellfish.

The invention discloses a dsRNA (double-stranded ribonucleic acid) for inhibiting expression of wheat aphid serine proteinase I gene and application thereof. The invention provides a dsRNA molecule disclosed as Sequence 2 in the sequence table. The invention also provides a DNA (deoxyribonucleic acid) molecule for coding the RNA molecule. The invention also provides application of the RNA molecule or the DNA molecule, which is at least one of the following (1) to (4): (1) preventing and treating aphids; (2) promoting death of aphids; (3) inhibiting growth of aphids; and (4) inhibiting expression of the aphid in-vivo serine proteinase I gene. The dsRNA has important application value in preventing and treating aphids in agricultural production.

The invention discloses a soilless culture nutrition formulation for loofah. The soilless culture nutrition formulation is composed of the following raw materials in parts by weight: 13-15 parts of Thalictrum glandulosissimum, 10-14 parts of lotus, 3-30 parts of rhizoma curculiginis, 4-5 parts of threonine, 4-5 parts of serine proteinase, 4-5 parts of cysteine proteinase, 1-3 parts of radix pseudostellariae, 1-3 parts of Pistachio, 1-3 parts of serine and the balance of water. The raw materials are weighed in proportion and are mixed uniformly. A nutrient solution provided by the invention has greatly enhanced seepage force and diffusivity when used for culturing seedlings, so that the seeding stage is greatly shortened, the emergence rate and the seedling quality are improved, and the cost is reduced; and the nutrition solution is suitable for high-altitude low-temperature seedling culture.

The present invention relates to a method for preparing medicine with the action capable of inhibiting proteniase and its detection method. Said method includes making the aqueous solution containing alpha 1-proteinase inhibitor undergo the pocesses of virus inactivation treatment, ion exchange resin adsorption and elution; at the same time using the reaction of serine proteinase and alpha 1-proteinase to result in photoabsorption change of correspondent wavelength so as to quantitatively determine the activity of alpha 1-proteinase inhibitor. The invented preparation method can effectively implement high yield, can ensure high purity and high activity of product, at the same time its process is simple, cost is low, and its detection method is simple and easy to implement, and its sensitivity is high.

The invention discloses a method for preparing functional raw materials from sea cucumbers, which comprises the following steps: step (a), preparing crushed sea cucumbers; and step (b), treating and reacting the crushed sea cucumbers with serine protease . The functional raw material of the present invention exhibits anti-inflammatory activity, whitening activity and wrinkle-improving activity superior to existing sea cucumber extracts.

The invention discloses a pumpkin soilless culture nutrition formula comprising the following materials by weight part: zaocys dhumnade 12-16 parts, licorice section 10-14 parts, abalone shell 2-20 parts, threonine 4-6 parts, serine proteinase 4-6 parts, thiol protease 4-6 parts, xiqiang 1-2 parts, pseudo-ginseng 1-2 parts, serine 1-2 parts, and balance water; said materials are weighted according to proportion and evenly mixed, thus obtaining the nutrient solution; the penetration and diffusivity of the nutrient solution can be greatly enhanced in seedling growing, thus greatly shortening the emergence period, improving emergence rate and emergence quality, and reducing cost; the nutrient solution is more suitable for high altitude low temperature seedling growing.

The invention discloses an expression optimization and purification method of helicobacter pylori serine proteinase. The expression optimization and purification method comprises the steps of constructing a target protein expression vector, performing induced expression of target protein under optimized condition, performing separation and purification on the target protein and the like. Accordingto the expression optimization and purification method disclosed by the invention, the target protein expression vector containing a His label is constructed and is successfully conveyed into host bacteria; besides, four factors of pre-inducing time, inducing temperature, the final concentration of an inducer and inducing time influencing growth of thalli of engineering bacteria and inducing expression quantity of the target protein are optimized through designing an orthogonal experiment, the optimal inducing expression condition is obtained, and the expression quantity of the target proteinis greatly increased; and an affinity chromatography method and an eluting system of the target protein are also optimized, so that the purity of the target protein reaches 98% and is notably increased than that by a traditional method, and the expression optimization and purification method has good application prospect on large-scale mass production of recombination protein.

The invention provides a template-fixed beta-hairpin peptidomimetics with protease inhibitory activity or salts thereof. The template-fixed beta-hairpin peptidomimetics have a general formula (I) as described in the specification, wherein Z is a chain of 11 alpha-amino acid residues which, depending on their positions in the chain (counted starting from the N-terminal amino acid), are Gly, or Pro, or Pro (4NHCOPhe), or of certain types which, as remaining symbols in the formula, are defined in the specification and the claims. The template-fixed beta-hairpin peptidomimetics have the property of inhibiting protease (especially serine proteinase, and in particular, cathepsin G, elastase, or tryptase). The beta-hairpin peptidomimetics can be produced through a method based on the strategy of hybrid solid phase-liquid phase synthesis.

The invention relates to a method for making and detecting protease inhibitor. The method comprises steps of: deactivating the water solution that contains alpha1-protease inhibitor with virus; ion exchanging resin absorbing and eluting; making serine proteinases react with alpha1-protease inhibitor to change wave length absorbed by light; measuring activity of thealpha1-protease inhibitor quantitatively. The invention has the advantages of being able to ensure product high purity and high activity.

The invention relates to an extracellular infectivity basic serine proteinase gene of a nematology bacillus and the application in the biotechnology field. The gene is got by colony from the nematology bacillus, wherein the length of open reading frame is 1146bp, the gene codes a protein with 382 amino acid residues which comprise a signal peptide with 30 amino acid residues, a front peptide with 77 amino acid residues and a mature peptide with 275 amino acid residues. The recombinant protein of the gene in the bacillus coli BL21 can degrade the body wall of the nematization and can kill the active of the nematization, and removing the mutant shows the reduction of the ptoteinase active and a large lost of the insecticidal activity. The basic serine proteinase gene is an important toxicity factor in the process that nematology bacillus infects nematization. The gene can be used to research the biochemistry essentiality of proteinase in the nfecting nematization process, which can be regarded as the target gene acquiring highly effective proof strain. The invention has the high safety and the good insecticidal effect.

The invention discloses a wood ear soilless cultivation nutrient formula. By weight, the formula comprises 13-16 parts of songyin, 10-14 parts of pinitol, 3-30 parts of frankincense, 4-6 parts of tryptophan, 4-6 parts of serine proteinase, 4-6 parts of thiol protease, 1-3 parts of auxin, 1-3 parts of abscisic acid, and 1-3 parts of serine, the balance being water. The materials are weighed according to the ratio, and the weighed materials are well mixed, such that a nutrient solution is obtained. When the nutrient solution is used in seedling raising, penetration and diffusion of the nutrient solution are greatly improved, such that an emergence period is greatly shortened, and emergence rate and seedling quality are improved. Also, cost is reduced. The nutrient solution is more suitable for high-altitude low-temperature seedling raising.

The invention discloses a soilless culture nutrition formula for tomatoes. The formula is prepared from the following ingredients in parts by weight: 13-16 parts of a Euphorbia named as Longgu, 10-14 parts of drynaria baronii, 3-30 parts of rhizoma curculiginis, 4-6 parts of tryptophan, 4-6 parts of serine proteinase, 4-6 parts of sulfhydryl protease, 1-3 parts of western rhizoma seu radix notopterygii, 1-3 parts of rhizoma pinelliae, 1-3 parts of serine and the balance of water. A nutrient solution is prepared by weighing ingredients in proportion, and mixing the ingredients uniformly. By virtue of the nutrient solution, the permeability and diffusibility of the nutrient solution are greatly enhanced during seedling culture, so that the period of emergence is greatly shortened, the rate of emergence and the seedling quality are improved, and the cost is also lowered; furthermore, the nutrient solution is more applicable to high-altitude low-temperature seedling culture.

The invention relates to the technical field of food processing, and more specifically relates to a method for producing deacidified lemon juice sugar. The method comprises the following steps: rubbing the fresh lemon to a paste state, inoculating serine proteinase for enzymatic hydrolysis, and using an enzymatic hydrolysis solution for producing the lemon juice sugar. According to the invention, deacidification is complete, a lot of glutathione is generated during a deacidification process, and the original nutrients in the lemon juice are abundant.