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Production of thrombolytic kinase (natto kinase) and its use

A technology of thrombokinase and thrombolysis, which is applied in the field of microorganisms, can solve the problems of complicated operation steps and complex extraction process of thrombolytic kinase, achieve obvious thrombolytic effect, enhance the effect of thrombosis prevention and treatment, and increase the production rate

Inactive Publication Date: 2007-04-04
SICHUAN ACAD OF FOOD & FERMENTATION INDS
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The fermentation ingredients and process adopted in the present invention improve the yield of submerged fermentation thrombolytic kinase, solve the problems of complex extraction process and complicated operation steps of present thrombolytic kinase, and provide a suitable method with effective, short process and simple steps. Method for Industrial Separation and Extraction of Thrombolytic Kinase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] Example 1 Production of Thrombolytic Kinase

[0032] After the Bacillus subtilis slant strain is activated, it is connected to the shake flask seed medium, cultivated at a constant temperature of 32 to 38°C at 150 to 250 rpm for 24 hours, and then connected to the fermentation medium, and the fermenter is fermented for 42 to 45 hours and then out of the tank. The average enzyme activity reaches 2100IU, pre-cooled to about 10°C, pumped into a two-stage microfiltration machine to collect the filtrate, and the enzyme activity yield is over 90%; The hollow fiber ultrafiltration concentration reaches more than 20 times, and the enzyme activity yield is more than 80%; the thrombolytic kinase concentrate is added with auxiliary materials and freeze-dried to obtain a solid powder with an enzyme activity of more than 20,000 IU / g.

example 2

[0033]Example 2 Thrombolytic Kinase Application (Taking the Capsule Finished Product as Example)

[0034] The thrombolytic kinase activity (equivalent to urokinase) produced by this technical scheme reaches more than 20,000 IU grams. Take 5,000 IU a day, take 2 times a day, and take 1 capsule each time. It can be directly loaded into enteric-coated capsules, and the capacity is adjusted by isomaltooligosaccharides. Each capsule contains 0.25 grams.

[0035] 1 gram of thrombolytic kinase (calculated at 20,000 IU / gram) freeze-dried powder, combined with 8 mg of functional auxiliary materials folic acid, 400 mg of Vc, VB 12 12 mg, 1 g of soybean lecithin, mix well, pack into 8 enteric-coated capsules.

[0036] The above-mentioned products, as health care products for people prone to cardiovascular and cerebrovascular diseases, are used for the prevention or auxiliary treatment of thrombotic cardiovascular and cerebrovascular diseases.

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Abstract

Production of natto kinase and its use are disclosed. The process is carried out by taking sugar, soy-bean and starch as raw materials, using hay bacillus high-yield thrombolytics kinase strain, deep liquid fermenting, adjusting metabolism by complex system, membrane separating, and freeze-drying to obtain the final product. It belongs to serine proteinase and can be used to melt thrombus, inhibit platelet coagulation, prevent hypertension and arteriosclerosis, adjust blood fat and keep blood flow unblocked, and it can be developed as infusion granule, tablet, capsule and oral liquid.

Description

technical field [0001] The invention proposes a fermentation method for producing thrombolytic kinase (nattokinase) and its application, which are scientific and technological inventions in the field of microorganism technology. The invention mainly relates to a method for improving the yield of thrombolytic kinase, a membrane separation and extraction method for thrombolytic kinase suitable for industrial production, and the application of a functionally compatible thrombolytic kinase composition for thrombolysis, auxiliary blood pressure lowering, and auxiliary blood lipid regulation. technical background [0002] Thrombolytic kinase is a water-soluble extracellular alkaline protease produced by the growth and metabolism of Bacillus subtilis. It was originally extracted from the traditional food natto by Japanese scholars in 1987 and named nattokinase. Since 1996, domestic scholars have also extracted thrombolytic kinase from natto-like foods (fermented foods such as tempe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N15/57A61K38/48A23L1/305A61K9/14A61K9/20A61K9/08A61K9/48A61P9/00A61P7/02A23L33/17
Inventor 程金权谢文渊康建平潘红梅王拥军
Owner SICHUAN ACAD OF FOOD & FERMENTATION INDS
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