203 results about "Nattokinase" patented technology

The invention relates to oral liquid for tonifying kidney and bone and a preparation method thereof, and belongs to the technical field of medicine, the oral liquid contains rhizoma drynariae, polygonum multiflorum, poria cocos, teasel root, radix paeoniae alba, angelica sinensis, codonopsis pilosula, prepared rehmannia root, polygonatum kingianum, fructus lycii, native copper and pericarpium citri reticulatae; the rhizoma drynariae is a product obtained by performing enzymolysis on a whole rhizoma drynariae plant through hemicellulase and pectinase, performing enzymolysis through bromelain and papain and performing enzymolysis through nattokinase; polygonum multiflorum is a product obtained by carrying out enzymolysis on Polygonum multiflorum tuberous roots through hemicellulase and pectinase, then carrying out enzymolysis through bromelain and papain and then carrying out enzymolysis through gelatinase. Polygonatum sibiricum is a product obtained by performing enzymolysis on rhizome of Polygonatum sibiricum through hemicellulase and pectinase, performing enzymolysis through bromelain and papain, and performing fermentation through bacillus licheniformis and saccharomyces boulardii. All the active components are matched for use, kidney function metabolism can be well enhanced, bone mineral density is increased, the treatment course is short, the effect taking speed is high, safety and reliability are achieved, and medication compliance is effectively improved.

The invention relates to a weight-losing enzyme and a preparation method thereof, and belongs to the technical field of biology, and the weight-losing enzyme comprises a fruit enzyme, a vegetable enzyme, a seed enzyme and a lotus leaf extract; the fruit enzyme is obtained by performing enzymolysis on mixed fruit pulp of apples, lemons, grapefruits and haws through pectinase, performing enzymolysis through pepsase and performing co-fermentation through bifidobacterium longum and lactobacillus acidophilus; the vegetable enzyme is obtained by performing enzymolysis on mixed vegetable pulp of wax gourd pulp, celery and spinach through pectinase, performing enzymolysis through pepsin and performing fermentation through lactobacillus plantarum and bifidobacterium adolescentis; the seed enzyme is obtained by carrying out trypsin enzymolysis on mixed powder of semen raphani and phaseolus calcaratus, then carrying out nattokinase enzymolysis and then fermenting with bifidobacterium breve; the lotus leaf extract is obtained by sequentially carrying out enzymolysis on lotus leaves through hemicellulase, snail protease and nattokinase. All the components are matched for use, so that the weight-losing food has the effects of promoting digestion, inhibiting fat absorption, regulating intestinal flora, promoting metabolism and the like, and has a good weight-losing effect.

The present application provides a soybean antihypertensive peptide and its screening method and application, which belongs to the field of polypeptides. The soybean antihypertensive peptide provided in the present application includes one or more of soybean antihypertensive peptide I with a molecular weight of less than 1000Da, soybean antihypertensive peptide II with a molecular weight of less than 1000Da, and soybean antihypertensive peptide III with a molecular weight of less than 1000Da; wherein, the soybean antihypertensive peptide I with a molecular weight of less than 1000Da is prepared by nattokinase enzymatic hydrolysis of soybean protein isolate, the soybean antihypertensive peptide III with a molecular weight of less than 1000Da is prepared by Bacillus subtilis liquid fermentation of soybean protein hydrolysate, and the soybean antihypertensive peptide III with a molecular weight of less than 1000Da is prepared by Bacillus subtilis solid fermentation of soybeans. The soybean antihypertensive peptide of the present application is a plant-derived antihypertensive peptide with high angiotensin converting enzyme inhibition rate and good antihypertensive effect. And it has been verified by animal experiments that the soybean antihypertensive peptide of the present application can significantly reduce the heart rate, diastolic blood pressure and systolic blood pressure of rats, and has less toxic and side effects.

The invention discloses an inulin gel compound loaded with Prussian blue nano-enzyme and nattokinase and application of the inulin gel compound in treatment of inflammatory bowel disease, the compound is formed by blending the Prussian blue nano-enzyme, the nattokinase and inulin, the Prussian blue nano-enzyme with multiple antioxidant enzyme activities is used for removing a large amount of active oxygen in cells, and the nattokinase is used for treating inflammatory bowel disease. The level of inflammatory factors is reduced, oxidative stress is relieved, and intestinal inflammation is treated; the nattokinase not only can inhibit the formation of intestinal microthrombus and effectively dissolve thrombus through the excellent thrombolysis capability of the nattokinase, but also can synergistically and effectively treat inflammatory bowel diseases through the intestinal microbiota regulation capability of the nattokinase; in addition, the inulin, the Prussian blue nano-enzyme and the nattokinase jointly realize the regulation of intestinal microbiota of the patient with the inflammatory bowel disease, so that the anxiety and depression symptoms related to the inflammatory bowel disease are effectively relieved through microbiota-intestine-brain axis. In general, the invention provides a creative example for treating the inflammatory bowel disease and related intestinal internal and external complications thereof.

The invention discloses a preparation method and application of yak blood peptide with anti-hypoxia and anti-fatigue functions. The method comprises the steps of raw material pretreatment, composite enzymolysis (adopting a mixture of enzyme digestion modified lumbrukinase and nattokinase), enzyme deactivation, separation and purification, ferrous chelation, Maillard reaction, concentration and drying and the like. The enzymolysis efficiency and the active peptide yield are remarkably improved through ultrasonic treatment and a composite enzymolysis technology, the stability and the anti-hypoxia capacity of peptide fragments are enhanced through ferrous chelation, and the antioxidant activity and the anti-fatigue activity are further improved through the Maillard reaction. Experiments show that the yak blood peptide prepared by the method can significantly improve the antioxidant enzyme activity, prolong the normal-pressure hypoxia survival time, prolong the swimming exhaustion time and significantly reduce the MDA content of brain tissue and myocardial tissue. The obtained product can be applied to anti-hypoxia and anti-fatigue medicines or health-care foods, and has the advantages of high process efficiency, remarkable activity, high safety and the like.

The invention discloses a ginseng and nattokinase composition and a preparation method thereof, belongs to the technical field of microbial fermentation, and provides the ginseng and nattokinase composition which comprises ginsenoside, ginseng peptide, acid-soluble protein, a stabilizer, an additive and a ginseng body. The preparation method comprises the following steps: S1, cleaning ginseng, crushing, cooking, cooling to 25-36 DEG C, and standing for 24 hours to obtain cooked ginseng; s2, adding an auxiliary agent and a stabilizer into the ginseng cooked in the step S1, then inoculating bacillus natto, and culturing for 24-36 hours at the temperature of 32-35 DEG C to obtain fermentation liquor; and S3, adding the auxiliary agent into the fermentation liquid in the step S2 again, uniformly mixing, culturing for 24-48 hours at the temperature of 36-40 DEG C, and then drying at low temperature to obtain the ginseng and nattokinase composition. The preparation method is simple and easy to popularize.

The invention relates to a skin wound repairing material and a preparation method thereof, and belongs to the technical field of biological medicines. The repair material disclosed by the invention contains palmitoyl tripeptide-1, methacrylated collagen, cuttlebone extract, recombinant human epidermal growth factor lipidosome, polydopamine nanoparticles, hyaluronic acid, sodium alginate modified silk fibroin and the like in a specific proportion. Wherein the cuttlebone extract is a product which is obtained by carrying out common enzymolysis on cuttlebone through chitinase and nattokinase and then carrying out step-by-step enzymolysis on the cuttlebone through gelatinase and lumbrukinase and is less than 5kDa. The components are prepared into an efficient product by adopting a special method, the stability of the components is improved, the problem that the activity is difficult to maintain is solved, and the skin wound repairing effect is good through reasonable compatibility.

The invention belongs to the technical field of health-care food, and particularly relates to a sacha inchi oil-based gel candy with functions of reducing hypertension, hyperglycemia and hyperlipidemia and a preparation method thereof, and the sacha inchi oil-based gel candy comprises the following components: 1) a main component: 30-70% of sacha inchi oil; 2) a blood fat reducing synergistic component which is one or a combination of more of 0.5%-5% of phytosterol, 0.1%-2% of a red yeast rice extract and 0.01%-0.5% of sugarcane octacosanol; 3) a hypoglycemic synergistic component, which comprises one or a combination of more of 5%-20% of inulin / fructo-oligosaccharide, 0.5%-5% of a bitter gourd extract, 0.5%-5% of a mulberry leaf extract and 10-200 [mu] g / grain of chromium; 4) an antihypertensive synergistic component, wherein the antihypertensive synergistic component is one or a combination of more of 500-5000FU / grain of nattokinase, 0.5-5% of a celery seed extract and 1-8% of a hawthorn extract; through scientific compounding, the sacha inchi oil and various synergistic components exert a combined effect, and meanwhile, auxiliary regulation is carried out on blood sugar, blood fat and blood pressure; the main components are derived from natural plant extracts or functional grease, so that the health-care product is high in safety and suitable for daily health care.

The invention discloses a method for producing nattokinase by solid-state fermentation of hemp seed meal. Hemp seed meal is used as the only matrix, nattokinase producing bacteria are inoculated on the solid culture medium, the temperature is controlled at 30-40°C, the humidity is controlled at 60-80°C, and the fermentation is carried out for 16-24 hours, and the enzyme production of nattokinase reaches 9592.8IU / g. The invention uses hemp seed meal, which is a waste of oil extraction, as the only fermentation matrix, greatly reduces the production cost of nattokinase, simplifies the fermentation process, and realizes the high-value utilization of hemp seed meal. The fresh fragrance of hemp seeds well neutralizes the ammonia smell generated during the fermentation process of nattokinase, making nattokinase more acceptable. The method is the first to use hemp seed meal solid-state fermentation to produce nattokinase, provides a new method for the production of nattokinase, and has broad application prospects.

The invention belongs to the technical field of food or medicine, and particularly relates to a preparation method of nattokinase sustained-release microspheres, which comprises the following steps: mixing an emulsifier span-80 with liquid paraffin to obtain an oil phase; adding chitosan into an acetic acid solution to prepare a chitosan water solution as a water phase; dropwise adding the water phase into the oil phase to prepare W / O type emulsion, dropwise adding a cross-linking agent A into the emulsion to obtain chitosan microspheres, and taking a chitosan microsphere nattokinase solution and a cross-linking agent B to obtain nattokinase-chitosan core microspheres; mixing the nattokinase-chitosan core microsphere with a cortisol aptamer solution, and incubating to enable the aptamer to be combined on the surface of the nattokinase-chitosan core microsphere; and adding a casein-PEG copolymer solution and a cross-linking agent C into the microspheres to obtain the cortisol response type intelligent switch microspheres. The nattokinase release amount can be dynamically adjusted day and night, the circadian thrombus risk rhythm is matched, the anti-thrombus effect is remarkably improved, side effects are reduced, and the nattokinase can be widely applied to the fields of functional food and drugs.

The invention discloses a uric acid reducing polypeptide, the polypeptide is derived from a peptide fragment, the'bindingerge 'value of the peptide fragment in molecular docking with xanthine oxidase is less than-1.5 kcal / mol, and the peptide fragment, in molecular docking with the xanthine oxidase, of the polypeptide is derived from a Peptide Ranker biological activity score gt; and 0.7 of a polypeptide. The uric acid reducing polypeptide is obtained by screening natto powder subjected to pepsin enzymolysis, natto powder enzymatic hydrolysate obtained through enzymolysis has the activity of inhibiting xanthine oxidase, the screened uric acid reducing polypeptide can be developed into a uric acid reducing medicine, and active ingredients are added into functional food and health food, so that the uric acid reducing polypeptide has a good application prospect. Good market prospects are realized in medicine and food industries.

The invention relates to the technical field of traditional Chinese medicine health-care products, and particularly discloses an earthworm protein peptide composition for improving vascular embolism and a preparation method thereof.The earthworm protein peptide composition is prepared from, by weight, 28-34 parts of earthworm protein peptide, 20-26 parts of active pharmaceutical ingredients, 1-5 parts of ginkgo biloba extract, 12-15 parts of corn oligopeptide powder and 4-8 parts of auxiliary materials; lumbricus protein peptide, active pharmaceutical ingredients, ginkgo biloba extract and corn oligopeptide powder are used as raw materials, a solubilizing component is added in the preparation process, the solubilizing component is prepared by mixing functional extract, nattokinase and tween-80 according to a certain proportion, the functional extract is prepared by coating grape seed extract with hydroxypropyl-beta-cyclodextrin, and the solubilizing component is prepared by mixing the functional extract, the nattokinase and the tween-80 according to a certain proportion. Through the synergistic effect of the components, the antithrombotic effect of the earthworm protein peptide composition is synergistically enhanced.

The invention relates to blackcurrant and orange juice assorted soda water, which belongs to the technical field of beverages, and contains blackcurrant extract, orange juice, white hyacinth bean seed extract, mogroside, stevioside and the like. Wherein the blackcurrant extract is a product which is less than 5kDa and is obtained by carrying out common enzymolysis on blackcurrants by hemicellulase and pectinase and then sequentially carrying out step-by-step enzymolysis on the blackcurrants by virtue of nattokinase, ficin, papain and glucokinase; the white hyacinth bean seed extract is a product below 5kDa obtained by performing enzymolysis on white hyacinth bean seeds through trypsin and then performing co-fermentation through propionibacterium freudenreichii, lactobacillus brevis and bifidobacterium longum; the blackcurrant extract and the white hyacinth bean seed extract have good effects of resisting fatigue, resisting oxidation and enhancing immunity, so that the assorted soda water is delicious and healthy.

The invention relates to the technical field of fermented bean products, in particular to fermented bean curd rich in nattokinase and a preparation method of the fermented bean curd. Comprising the following steps: carrying out strain activation and enlarged culture on bacillus natto, centrifuging, and removing supernatant to obtain a bacillus natto precipitate; uniformly mixing mucor and bacillus natto precipitates with sterile water in proportion to prepare a strain suspension; preparing a bean curd blank, spraying the strain suspension, and carrying out primary fermentation to obtain a bean curd blank; spreading table salt on the surface of the bean curd blank to pickle the bean curd blank to obtain a salted blank; preparing a soup base, inoculating the bacillus natto precipitate, fermenting, and preparing a fermented bean curd soup base; and putting the salt blank and the fermented bean curd soup base into a container, sealing, and carrying out post-fermentation under the condition of constant temperature, so as to obtain the fermented bean curd rich in nattokinase. The method has the advantages that the biological activity of the nattokinase in a salt environment is improved by a method of reducing the use amount of the salt and performing multiple fermentation on the bacillus natto; and a fermentation microbial enzyme system is enriched.

The invention discloses natto oligopeptide capable of relieving formation of arterial plaques and a preparation method and application thereof, belongs to the field of active peptide preparation, aims to solve the problem of obtaining natto or oligopeptide thereof capable of relieving formation of atherosclerotic plaques, and is characterized in that soybeans are soaked, sterilized, inoculated with compound bacteria, fermented at constant temperature and subjected to after-ripening to obtain natto, the volume ratio of the bacillus natto to the lactobacillus plantarum to the bifidobacterium bifidum in the compound bacteria is 1: (0.3-0.5): (0.1-0.3); compound protease is added into the aqueous solution of the freeze-dried natto powder for hydrolysis, centrifugation and filtration, components below 30000 Da are intercepted to obtain the natto oligopeptide, the ratio of chymotrypsin to papain in the compound protease is (1-3): 1, and the natto oligopeptide has the effects that the content of nattokinase in natto is higher, the protein level is also improved, and the natto oligopeptide can be used for preparing the natto oligopeptide. The nattokinase activity is well reserved in hydrolysis, the effect of promoting macrophage autophagy is achieved, and atherosclerotic plaque formation can be relieved.

The invention relates to application of microalgae polysaccharide in improving intestinal immunity of dairy cows, belongs to the technical field of feeds, and the microalgae polysaccharide prepared by the invention is used for improving the secretion volume of intestinal mucosa to lgA, lgG and lgM. Kelp buds, dunaliella salina and haematococcus pluvialis are compounded and mixed according to a specific proportion, trypsin and nattokinase are adopted for pre-enzymolysis within a certain period of time, then hemicellulase and pectinase are adopted for enzymolysis within a certain period of time, and the enzymolysis effect is good. The obtained microalgae polysaccharide product has an excellent effect of promoting the intestinal tract of the dairy cow to secrete immune globulin, the content of IgA, IgG and IgM in the intestinal tract of the dairy cow can be greatly increased, the immunity of the intestinal tract of the dairy cow is further improved, and the microalgae polysaccharide product can be used as a substitute component for efficiently improving the immune function of the intestinal tract of the dairy cow.

The invention relates to the technical field of biological medicines, in particular to a preparation method of slow-release acid-resistant nattokinase, which adopts a preparation process that nattokinase is solidified and embedded in a core material with a three-dimensional network structure, and a surface cross-linking agent and a novel slow-release agent are combined to form an outer layer structure. The gastric acid resistance and the slow-release effect of the nattokinase are effectively improved. The preparation process is simple, mild in condition and suitable for industrial production.

The invention relates to a biological fermentation organic fertilizer based on crop residues and a fermentation method and application, and belongs to the technical field of organic fertilizer production.The fermentation method of the biological fermentation organic fertilizer based on the crop residues comprises the steps that firstly, the crop residues are pretreated to obtain a premixed material A and a premixed material B; mixing saccharomycetes, lactic acid bacteria, bacillus laterosporus, flavobacterium flagelloides, cellulase, xylanase and nattokinase to obtain an enzyme and bacterium mixture, composting the premixed material A and the premixed material B, and mixing with the enzyme and bacterium mixture for fermentation. According to the biological fermentation organic fertilizer based on the crop residues, the crop residues are pretreated through multiple means before fermentation, and a specific enzyme and bacterium mixing agent is mixed in the fermentation process to promote fermentation of the crop residues, so that after the organic fertilizer acts on soil, the content of organic matter in the soil can be increased, and the organic matter content in the soil can be increased; and heavy metals in the soil can be obviously reduced.

The invention relates to a composition for bone repair and growth and a preparation method thereof, and belongs to the technical field of medicines, the composition comprises a glue layer extracted peptide in sandjellyfish and a tabasheer-grifola frondosa composite bacterial peptide; the extract peptide of the glue layer in the sand jellyfish contains a product between 500Da and 2000Da, wherein the product is obtained by sequentially carrying out reaction on the glue layer in the sand jellyfish through lumbrukinase, serrapeptase and glutamine transaminase; the tabasheer-grifola frondosa composite bacterial peptide contains a product between 500Da and 2000Da, which is obtained by performing common enzymolysis on tabasheer mushrooms and grifola frondosa mushrooms through cellulase and chitinase, then performing common enzymolysis on the tabasheer mushrooms and the grifola frondosa mushrooms through nattokinase and serrapeptase and finally performing common reaction on aminopeptidase and glutamine transaminase. According to the invention, a special method is adopted to prepare the glue layer extracted peptide and the tabasheer-grifola frondosa compound bacterium peptide in the sandfish, and the glue layer extracted peptide and the tabasheer-grifola frondosa compound bacterium peptide are matched for use to achieve good biological activity, stability and absorption and utilization rate, so that the defects of the existing skeleton repair and growth medicines are overcome, and the repair and growth of skeletons are effectively promoted.

The invention discloses a process for producing nattokinase by fermenting quinoa with bacillus natto, and belongs to the technical field of fermentation engineering. The method is realized through the four steps of pretreatment, inoculation and fermentation, nattokinase extraction and quality detection. The optimal fermentation time, temperature, inoculum size, cooking time and soaking time are determined through experiments. The conditions are favorable for maximizing the activity of nattokinase, ensuring that the tianzhu chenopodium quinoa is fermented under the most appropriate conditions, and effectively improving the yield and the quality of the tianzhu chenopodium quinoa. The steps of ethanol extraction and centrifugation are combined, so that impurities can be effectively removed, and the purity of an extracting solution is improved. According to the method, high-purity nattokinase can be obtained, and the quality and stability of the product are ensured. In the soaking and fermentation process, the temperature and humidity are monitored in real time and adjusted in good time, so that the uniform heating of the tianzhu chenopodium quinoa willd and the stability of the fermentation environment are ensured, and the fermentation efficiency and the product consistency are improved.

The invention relates to a pig immunity-enhancing feed containing natural plant extracts, and belongs to the technical field of feeds. On the basis of basic components, a plantain herb and selfheal composite extract, a purslane and sweet potato vine and leaf composite extract and a folium cortex eucommiae extract are also added into the feed, so that the immunity-enhancing feed is prepared. The plantain herb and selfheal composite extract is obtained by sequentially fermenting a plantain herb whole plant and a selfheal whole plant through lactobacillus cellobiosus and pediococcus acidilactici and then sequentially carrying out step-by-step enzymolysis through trypsin and nattokinase; the herba portulacae and sweet potato vine compound extract is obtained by sequentially fermenting a whole plant of herba portulacae and leaves of sweet potato vines with lactobacillus cellobiosus and bacteroides ruminalis and then sequentially carrying out step-by-step enzymolysis with snail protease and trypsin; the folium cortex eucommiae extract is obtained by sequentially carrying out step-by-step enzymolysis on folium cortex eucommiae through hemicellulase, bromelain and nattokinase. The extract prepared by the invention contains immunological enhancement effective components, and can enhance the immunity of pigs, effectively reduce diarrhea and reduce the risk of illness when being matched with basal feed for use.

The invention particularly discloses a preparation process of freeze-dried nattokinase. The preparation process of the freeze-dried nattokinase comprises the following steps: S1, mixing a nattokinase stock solution with a composite freeze-drying protective agent to obtain a mixed solution; s2, performing pre-freezing treatment operation on the mixed solution twice to obtain a pre-frozen product; s3, carrying out sublimation treatment operation on the pre-frozen product at a sublimation rate of 0.1-0.5 g / h.cm < 2 >, a sublimation temperature of-30 DEG C to-10 DEG C and a sublimation vacuum degree of 20-25 Pa to obtain a sublimation treatment product; S4, carrying out desorption drying treatment operation on the sublimation treatment product at a drying temperature of 15-20 DEG C and a drying vacuum degree of 20-30 Pa to obtain a sublimation treatment product; freeze-drying the obtained nattokinase; wherein the composite freeze-drying protective additive comprises sugar, amino acid, an antioxidant and an excipient. The nattokinase freeze-drying agent has the advantage of improving the freeze-drying activity and storage stability of nattokinase.

The present invention relates to a recombinant genetic engineering bacterium for producing nattokinase and its application, belonging to the field of genetic engineering technology. A signal peptide is modified and the modified signal peptide is screened to obtain a signal peptide, the sequence of which is shown in SEQ ID NO.4. A high-expression strain of Bacillus subtilis nattokinase is constructed to improve the activity of protease.

The present invention relates to a pig immune-enhancing feed containing natural plant extracts, belonging to the technical field of feeds. On the basis of the basic components, the feed of the present invention also adds a composite extract of plantain and selfheal, a composite extract of purslane and sweet potato vine leaves, and eucommia leaf extract. The composite extract of plantain and selfheal is obtained by successively fermenting the whole plant of plantain and the whole plant of selfheal with Lactobacillus cellobiosus and Pediococcus acidilactici, and then successively subjecting them to enzymatic hydrolysis with trypsin and nattokinase step by step; the composite extract of purslane and sweet potato vine leaves is obtained by successively fermenting the whole plant of purslane and sweet potato vine leaves with Lactobacillus cellobiosus and Bacteroides ruminicola, and then successively subjecting them to enzymatic hydrolysis with snail protease and trypsin step by step; the eucommia leaf extract is obtained by successively subjecting eucommia leaves to enzymatic hydrolysis with hemicellulase, bromelain, and nattokinase step by step. The extract contains immune-enhancing active ingredients, and when used in combination with the basic feed, it can enhance the immunity of pigs, effectively reduce diarrhea, and reduce the risk of disease.

The invention discloses fermented soybean meal and a preparation method thereof, and belongs to the technical field of fermented food. After soybean meal and soybean slurry are mixed, bacillus natto and kluyveromyces marxianus are inoculated for fermentation, and the fermented soybean meal can be obtained. According to the method, bacillus natto and kluyveromyces marxianus are used as fermentation strains, a soybean meal processing technology is optimized through a single-bacterium and mixed-bacterium fermentation technology, the influence of fermentation on physicochemical properties, functional activity and flavor quality of soybean meal is systematically explored, a high-value fermented soybean meal product is prepared, and the nattokinase (NK) activity of the mixed-bacterium fermented soybean meal reaches 6562.49 IU / g. High-activity nattokinase can be produced by fermenting soybean meal with bacillus natto, and the flavor of the fermented soybean meal can be improved and the nutritional value of the soybean meal can be improved by mixed fermentation with kluyveromyces cerevisiae. According to the technical scheme, high-value utilization of soybean meal resources can be promoted, the cost of terminal products can be reduced, and dependence on soybean import can be reduced.

The invention discloses a preparation and production process of natto soybean milk, which comprises the following steps: (1) preparing soybean milk: preparing soybeans into soybean milk, conveying the soybean milk into a first vacuum heat preservation tank, and cooling the soybean milk to 37 DEG C; (2) auxiliary material pretreatment: preparing sugar, a thickening agent and clear water, putting the sugar, the thickening agent and the clear water into a second vacuum heat preservation tank, sterilizing the interior of the second vacuum heat preservation tank, and cooling to 37 DEG C to prepare premixed auxiliary material liquid; (3) performing primary fermentation, namely aseptically conveying the premixed auxiliary material liquid prepared in the step (2) into a first vacuum heat preservation tank in a pipeline manner, adding bacillus natto into the first vacuum heat preservation tank, and performing micro-pressure fermentation for 24 hours under the pressure condition of 0.05-0.10 MPa to prepare nattokinase; and (4) secondary fermentation: cooling the nattokinase prepared in the step (3) to 20-28 DEG C, adding mucor into the first vacuum heat preservation tank, and performing micro-pressure fermentation for 24 hours to prepare the natto soybean milk.

The invention provides natto as well as a preparation method, health-care food and application thereof. The preparation method comprises the following steps: soaking soybeans; sterilizing the soaked soybeans; inoculating the sterilized soybeans with bacillus natto for first-stage fermentation, and then adding lactose into a fermentation system for second-stage fermentation to obtain natto; wherein the addition amount of the lactose is that 1-10% of the lactose is added on the basis that the mass of the dry beans is 100%. According to the method, special odor of traditional natto is removed, and the prepared natto powder has good nattokinase activity and a remarkable blood sugar reducing effect.

The invention discloses a nattokinase product for preventing and / or treating atherosclerosis and application of the nattokinase product. Cardiovascular diseases become the primary reason of human death, and the vascular plaque problem caused by atherosclerosis is always a difficult problem to be solved urgently. Blood vessel stenosis caused by plaques is a main cause of cerebral arterial thrombosis, and traditional medicines and operations face the problems of low compliance, side effect risk, indication limitation and the like. The invention provides nattokinase or a product thereof for high-risk groups with cardiovascular diseases, and the nattokinase or the product thereof is preferably in a non-drug form. As a precise nutrition intervention means, nattokinase is effective in plaque progress inhibition, unique in effect, safe to eat, convenient, easy to implement and wide in applicable crowd. The invention provides a new thought and method for management of vascular plaques and prevention and treatment of cardiovascular diseases.

The invention relates to the technical field of microbial fermentation engineering, and particularly discloses natto red yeast rice herb bean flour and a preparation method thereof. The preparation method comprises the following steps: respectively activating specifically preserved bacillus natto and monascus to obtain secondary seed solutions; inoculating the two seed solutions into a solid-state fermentation culture medium for co-fermentation; and inactivating, drying and crushing the fermentation product to obtain a finished product. The solid-state fermentation culture medium is prepared by compounding a bean matrix (soybeans, black beans or chickpeas) and herb tea components (roses, lotus leaves, chrysanthemums, mulberry leaves, haws, cassia seeds and the like). Through the synergistic effect of the bacillus natto and the monascus, nattokinase and monacolin K are efficiently enriched in a single fermentation system at the same time, conversion of soy isoflavone to a high-bioavailability aglycone type is remarkably promoted, and the obtained product has multiple health-care functions of thrombolysis, lipid reduction, efficient absorption and the like.

The present invention relates to a pharmaceutical composition for treating peptic gastric ulcer and a preparation method thereof, belonging to the field of biotechnology. The pharmaceutical composition contains chicken gizzard-membrane peptide, pumpkin seed extract, complex bacteria, white hyacinth bean flower extract and bletilla striata extract; the chicken gizzard-membrane peptide contains the products obtained by successively subjecting chicken gizzard-membrane to fractional enzymolysis with pepsin, chymotrypsin and keratinase; the pumpkin seed extract contains the products obtained by subjecting pumpkin seeds to co-enzymolysis with cellulase and pectinase and then successively to fractional enzymolysis with pepsin, nattokinase and aminopeptidase; the complex bacteria contain the co-fermentation bacteria of Lactobacillus acidophilus, Bifidobacterium and Lactobacillus casei; the white hyacinth bean flower extract is an ethanol percolation extract; the bletilla striata extract contains the products obtained by subjecting bletilla striata tubers to co-enzymolysis with cellulase and pectinase and then successively to fractional enzymolysis with pepsin, bromelain and serrapeptase. The various components are used in combination, having a good therapeutic effect on gastric ulcer, quickly and efficiently repairing gastric mucosa, and being safe and free of side effects.