108 results about "Nattokinase" patented technology

The invention discloses a preparation method and application of yak blood peptide with anti-hypoxia and anti-fatigue functions. The method comprises the steps of raw material pretreatment, composite enzymolysis (adopting a mixture of enzyme digestion modified lumbrukinase and nattokinase), enzyme deactivation, separation and purification, ferrous chelation, Maillard reaction, concentration and drying and the like. The enzymolysis efficiency and the active peptide yield are remarkably improved through ultrasonic treatment and a composite enzymolysis technology, the stability and the anti-hypoxia capacity of peptide fragments are enhanced through ferrous chelation, and the antioxidant activity and the anti-fatigue activity are further improved through the Maillard reaction. Experiments show that the yak blood peptide prepared by the method can significantly improve the antioxidant enzyme activity, prolong the normal-pressure hypoxia survival time, prolong the swimming exhaustion time and significantly reduce the MDA content of brain tissue and myocardial tissue. The obtained product can be applied to anti-hypoxia and anti-fatigue medicines or health-care foods, and has the advantages of high process efficiency, remarkable activity, high safety and the like.

The invention relates to a skin wound repairing material and a preparation method thereof, and belongs to the technical field of biological medicines. The repair material disclosed by the invention contains palmitoyl tripeptide-1, methacrylated collagen, cuttlebone extract, recombinant human epidermal growth factor lipidosome, polydopamine nanoparticles, hyaluronic acid, sodium alginate modified silk fibroin and the like in a specific proportion. Wherein the cuttlebone extract is a product which is obtained by carrying out common enzymolysis on cuttlebone through chitinase and nattokinase and then carrying out step-by-step enzymolysis on the cuttlebone through gelatinase and lumbrukinase and is less than 5kDa. The components are prepared into an efficient product by adopting a special method, the stability of the components is improved, the problem that the activity is difficult to maintain is solved, and the skin wound repairing effect is good through reasonable compatibility.

The invention belongs to the technical field of health-care food, and particularly relates to a sacha inchi oil-based gel candy with functions of reducing hypertension, hyperglycemia and hyperlipidemia and a preparation method thereof, and the sacha inchi oil-based gel candy comprises the following components: 1) a main component: 30-70% of sacha inchi oil; 2) a blood fat reducing synergistic component which is one or a combination of more of 0.5%-5% of phytosterol, 0.1%-2% of a red yeast rice extract and 0.01%-0.5% of sugarcane octacosanol; 3) a hypoglycemic synergistic component, which comprises one or a combination of more of 5%-20% of inulin / fructo-oligosaccharide, 0.5%-5% of a bitter gourd extract, 0.5%-5% of a mulberry leaf extract and 10-200 [mu] g / grain of chromium; 4) an antihypertensive synergistic component, wherein the antihypertensive synergistic component is one or a combination of more of 500-5000FU / grain of nattokinase, 0.5-5% of a celery seed extract and 1-8% of a hawthorn extract; through scientific compounding, the sacha inchi oil and various synergistic components exert a combined effect, and meanwhile, auxiliary regulation is carried out on blood sugar, blood fat and blood pressure; the main components are derived from natural plant extracts or functional grease, so that the health-care product is high in safety and suitable for daily health care.

The invention belongs to the technical field of food or medicine, and particularly relates to a preparation method of nattokinase sustained-release microspheres, which comprises the following steps: mixing an emulsifier span-80 with liquid paraffin to obtain an oil phase; adding chitosan into an acetic acid solution to prepare a chitosan water solution as a water phase; dropwise adding the water phase into the oil phase to prepare W / O type emulsion, dropwise adding a cross-linking agent A into the emulsion to obtain chitosan microspheres, and taking a chitosan microsphere nattokinase solution and a cross-linking agent B to obtain nattokinase-chitosan core microspheres; mixing the nattokinase-chitosan core microsphere with a cortisol aptamer solution, and incubating to enable the aptamer to be combined on the surface of the nattokinase-chitosan core microsphere; and adding a casein-PEG copolymer solution and a cross-linking agent C into the microspheres to obtain the cortisol response type intelligent switch microspheres. The nattokinase release amount can be dynamically adjusted day and night, the circadian thrombus risk rhythm is matched, the anti-thrombus effect is remarkably improved, side effects are reduced, and the nattokinase can be widely applied to the fields of functional food and drugs.

The invention discloses a uric acid reducing polypeptide, the polypeptide is derived from a peptide fragment, the'bindingerge 'value of the peptide fragment in molecular docking with xanthine oxidase is less than-1.5 kcal / mol, and the peptide fragment, in molecular docking with the xanthine oxidase, of the polypeptide is derived from a Peptide Ranker biological activity score gt; and 0.7 of a polypeptide. The uric acid reducing polypeptide is obtained by screening natto powder subjected to pepsin enzymolysis, natto powder enzymatic hydrolysate obtained through enzymolysis has the activity of inhibiting xanthine oxidase, the screened uric acid reducing polypeptide can be developed into a uric acid reducing medicine, and active ingredients are added into functional food and health food, so that the uric acid reducing polypeptide has a good application prospect. Good market prospects are realized in medicine and food industries.

The invention relates to the technical field of traditional Chinese medicine health-care products, and particularly discloses an earthworm protein peptide composition for improving vascular embolism and a preparation method thereof.The earthworm protein peptide composition is prepared from, by weight, 28-34 parts of earthworm protein peptide, 20-26 parts of active pharmaceutical ingredients, 1-5 parts of ginkgo biloba extract, 12-15 parts of corn oligopeptide powder and 4-8 parts of auxiliary materials; lumbricus protein peptide, active pharmaceutical ingredients, ginkgo biloba extract and corn oligopeptide powder are used as raw materials, a solubilizing component is added in the preparation process, the solubilizing component is prepared by mixing functional extract, nattokinase and tween-80 according to a certain proportion, the functional extract is prepared by coating grape seed extract with hydroxypropyl-beta-cyclodextrin, and the solubilizing component is prepared by mixing the functional extract, the nattokinase and the tween-80 according to a certain proportion. Through the synergistic effect of the components, the antithrombotic effect of the earthworm protein peptide composition is synergistically enhanced.

The invention relates to the technical field of fermented bean products, in particular to fermented bean curd rich in nattokinase and a preparation method of the fermented bean curd. Comprising the following steps: carrying out strain activation and enlarged culture on bacillus natto, centrifuging, and removing supernatant to obtain a bacillus natto precipitate; uniformly mixing mucor and bacillus natto precipitates with sterile water in proportion to prepare a strain suspension; preparing a bean curd blank, spraying the strain suspension, and carrying out primary fermentation to obtain a bean curd blank; spreading table salt on the surface of the bean curd blank to pickle the bean curd blank to obtain a salted blank; preparing a soup base, inoculating the bacillus natto precipitate, fermenting, and preparing a fermented bean curd soup base; and putting the salt blank and the fermented bean curd soup base into a container, sealing, and carrying out post-fermentation under the condition of constant temperature, so as to obtain the fermented bean curd rich in nattokinase. The method has the advantages that the biological activity of the nattokinase in a salt environment is improved by a method of reducing the use amount of the salt and performing multiple fermentation on the bacillus natto; and a fermentation microbial enzyme system is enriched.

The invention relates to a composition for bone repair and growth and a preparation method thereof, and belongs to the technical field of medicines, the composition comprises a glue layer extracted peptide in sandjellyfish and a tabasheer-grifola frondosa composite bacterial peptide; the extract peptide of the glue layer in the sand jellyfish contains a product between 500Da and 2000Da, wherein the product is obtained by sequentially carrying out reaction on the glue layer in the sand jellyfish through lumbrukinase, serrapeptase and glutamine transaminase; the tabasheer-grifola frondosa composite bacterial peptide contains a product between 500Da and 2000Da, which is obtained by performing common enzymolysis on tabasheer mushrooms and grifola frondosa mushrooms through cellulase and chitinase, then performing common enzymolysis on the tabasheer mushrooms and the grifola frondosa mushrooms through nattokinase and serrapeptase and finally performing common reaction on aminopeptidase and glutamine transaminase. According to the invention, a special method is adopted to prepare the glue layer extracted peptide and the tabasheer-grifola frondosa compound bacterium peptide in the sandfish, and the glue layer extracted peptide and the tabasheer-grifola frondosa compound bacterium peptide are matched for use to achieve good biological activity, stability and absorption and utilization rate, so that the defects of the existing skeleton repair and growth medicines are overcome, and the repair and growth of skeletons are effectively promoted.

The invention particularly discloses a preparation process of freeze-dried nattokinase. The preparation process of the freeze-dried nattokinase comprises the following steps: S1, mixing a nattokinase stock solution with a composite freeze-drying protective agent to obtain a mixed solution; s2, performing pre-freezing treatment operation on the mixed solution twice to obtain a pre-frozen product; s3, carrying out sublimation treatment operation on the pre-frozen product at a sublimation rate of 0.1-0.5 g / h.cm < 2 >, a sublimation temperature of-30 DEG C to-10 DEG C and a sublimation vacuum degree of 20-25 Pa to obtain a sublimation treatment product; S4, carrying out desorption drying treatment operation on the sublimation treatment product at a drying temperature of 15-20 DEG C and a drying vacuum degree of 20-30 Pa to obtain a sublimation treatment product; freeze-drying the obtained nattokinase; wherein the composite freeze-drying protective additive comprises sugar, amino acid, an antioxidant and an excipient. The nattokinase freeze-drying agent has the advantage of improving the freeze-drying activity and storage stability of nattokinase.

The present invention relates to a pig immune-enhancing feed containing natural plant extracts, belonging to the technical field of feeds. On the basis of the basic components, the feed of the present invention also adds a composite extract of plantain and selfheal, a composite extract of purslane and sweet potato vine leaves, and eucommia leaf extract. The composite extract of plantain and selfheal is obtained by successively fermenting the whole plant of plantain and the whole plant of selfheal with Lactobacillus cellobiosus and Pediococcus acidilactici, and then successively subjecting them to enzymatic hydrolysis with trypsin and nattokinase step by step; the composite extract of purslane and sweet potato vine leaves is obtained by successively fermenting the whole plant of purslane and sweet potato vine leaves with Lactobacillus cellobiosus and Bacteroides ruminicola, and then successively subjecting them to enzymatic hydrolysis with snail protease and trypsin step by step; the eucommia leaf extract is obtained by successively subjecting eucommia leaves to enzymatic hydrolysis with hemicellulase, bromelain, and nattokinase step by step. The extract contains immune-enhancing active ingredients, and when used in combination with the basic feed, it can enhance the immunity of pigs, effectively reduce diarrhea, and reduce the risk of disease.

The invention discloses fermented soybean meal and a preparation method thereof, and belongs to the technical field of fermented food. After soybean meal and soybean slurry are mixed, bacillus natto and kluyveromyces marxianus are inoculated for fermentation, and the fermented soybean meal can be obtained. According to the method, bacillus natto and kluyveromyces marxianus are used as fermentation strains, a soybean meal processing technology is optimized through a single-bacterium and mixed-bacterium fermentation technology, the influence of fermentation on physicochemical properties, functional activity and flavor quality of soybean meal is systematically explored, a high-value fermented soybean meal product is prepared, and the nattokinase (NK) activity of the mixed-bacterium fermented soybean meal reaches 6562.49 IU / g. High-activity nattokinase can be produced by fermenting soybean meal with bacillus natto, and the flavor of the fermented soybean meal can be improved and the nutritional value of the soybean meal can be improved by mixed fermentation with kluyveromyces cerevisiae. According to the technical scheme, high-value utilization of soybean meal resources can be promoted, the cost of terminal products can be reduced, and dependence on soybean import can be reduced.

The invention provides natto as well as a preparation method, health-care food and application thereof. The preparation method comprises the following steps: soaking soybeans; sterilizing the soaked soybeans; inoculating the sterilized soybeans with bacillus natto for first-stage fermentation, and then adding lactose into a fermentation system for second-stage fermentation to obtain natto; wherein the addition amount of the lactose is that 1-10% of the lactose is added on the basis that the mass of the dry beans is 100%. According to the method, special odor of traditional natto is removed, and the prepared natto powder has good nattokinase activity and a remarkable blood sugar reducing effect.

The invention discloses a nattokinase product for preventing and / or treating atherosclerosis and application of the nattokinase product. Cardiovascular diseases become the primary reason of human death, and the vascular plaque problem caused by atherosclerosis is always a difficult problem to be solved urgently. Blood vessel stenosis caused by plaques is a main cause of cerebral arterial thrombosis, and traditional medicines and operations face the problems of low compliance, side effect risk, indication limitation and the like. The invention provides nattokinase or a product thereof for high-risk groups with cardiovascular diseases, and the nattokinase or the product thereof is preferably in a non-drug form. As a precise nutrition intervention means, nattokinase is effective in plaque progress inhibition, unique in effect, safe to eat, convenient, easy to implement and wide in applicable crowd. The invention provides a new thought and method for management of vascular plaques and prevention and treatment of cardiovascular diseases.

The invention relates to the technical field of microbial fermentation engineering, and particularly discloses natto red yeast rice herb bean flour and a preparation method thereof. The preparation method comprises the following steps: respectively activating specifically preserved bacillus natto and monascus to obtain secondary seed solutions; inoculating the two seed solutions into a solid-state fermentation culture medium for co-fermentation; and inactivating, drying and crushing the fermentation product to obtain a finished product. The solid-state fermentation culture medium is prepared by compounding a bean matrix (soybeans, black beans or chickpeas) and herb tea components (roses, lotus leaves, chrysanthemums, mulberry leaves, haws, cassia seeds and the like). Through the synergistic effect of the bacillus natto and the monascus, nattokinase and monacolin K are efficiently enriched in a single fermentation system at the same time, conversion of soy isoflavone to a high-bioavailability aglycone type is remarkably promoted, and the obtained product has multiple health-care functions of thrombolysis, lipid reduction, efficient absorption and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to application of a nattokinase preparation in preparation of a medicine for preventing or treating sepsis. The nattokinase preparation comprises an electrostatic complex prepared from nattokinase and an anionic compound, the mass ratio of the nattokinase to the anionic compound ranges from 1: 1 to 1: 32, and the anionic compound is selected from one or two of polysialic acid or hyaluronic acid. Research results show that the nattokinase preparation not only can significantly improve the survival rate and weight of sepsis mice, but also can effectively stimulate the organism to generate potent and lasting immune memory response, and enhance the risk of the mice to resist secondary or more lipopolysaccharide attacks.

This invention discloses a small-molecule soybean peptide, its preparation method, and its applications. The preparation method includes: mixing soybean protein isolate with water and then performing pH shift pretreatment, deep eutectic solvent pretreatment, and pulsed electric field pretreatment; subsequently, enzymatic hydrolysis, enzyme inactivation, centrifugation, and collection of the supernatant; then, performing a two-step fractional alcohol precipitation treatment on the supernatant, collecting the precipitate, reconstituteing, and freeze-drying to obtain the small-molecule soybean peptide. This soybean peptide has a molecular weight <1 kDa and a component content of over 94%, and can be used as a nitrogen source to replace soybean peptone in Bacillus subtilis fermentation medium. This small-molecule soybean peptide has a good yield-promoting effect on at least five Bacillus subtilis strains and can be used to promote the production of nattokinase by Bacillus subtilis biofilm fermentation, with the highest nattokinase activity reaching approximately 14800 U / mL.

The invention belongs to the technical field of microorganisms, and discloses bacillus subtilis with a high nattokinase yield and a biological preservation function and application of the bacillus subtilis. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34222. The serine protease gene of the strain carries specific mutations (SEQ ID NO: 1) which are mutated from G to C at the 217th site and the 230th site, so that the yield and the activity of nattokinase are remarkably improved. In the fermentation process, through an induction stage (38 DEG C heat preservation for 8 hours), a main fermentation stage (50 DEG C heat preservation for 7 hours) and a cooling after-ripening stage (4 DEG C heat preservation for 24 hours), the enzyme activity of the prepared natto product is larger than or equal to 57028 IU / mL, and the natto product is light in flavor, excellent in wiredrawing effect and appropriate in taste. In addition, the strain can inhibit the growth of Candida albicans (the inhibition zone is greater than or equal to 25mm), and the shelf life of natto is effectively prolonged. The problems that an existing strain is poor in stability, products are prone to pollution, and the flavor is poor are solved.

The invention relates to a composition for preventing cardiovascular and cerebrovascular diseases and a preparation method thereof, and belongs to the technical field of health food. The composition for preventing cardiovascular and cerebrovascular diseases is prepared from the following components in parts by weight: 20 to 100 parts of dihydroquercetin, 100 to 300 parts of red yeast rice, 50 to 150 parts of nattokinase, 50 to 200 parts of earthworm protein, 100 to 300 parts of hawthorn fruit extract, 100 to 300 parts of radix puerariae extract and 100 to 300 parts of sea-buckthorn extract. The composition prepared by the invention has the characteristics of lowering lipid, dissolving thrombus and protecting heart and blood vessels.

The present invention relates to a composition for bone repair and growth and a preparation method thereof, belonging to the field of medical technology. The composition includes a peptide extracted from the gelatinous layer of sand jellyfish and a bamboo yellow-Grifola frondosa composite bacterial peptide; the peptide extracted from the gelatinous layer of sand jellyfish contains a product between 500Da and 2000Da obtained by sequentially hydrolyzing the gelatinous layer of sand jellyfish with lumbrokinase, serrapeptase, and transglutaminase; the bamboo yellow-Grifola frondosa composite bacterial peptide contains a product between 500Da and 2000Da obtained by co-enzymatic hydrolysis of bamboo yellow mushrooms and Grifola frondosa mushrooms with cellulase and chitinase, then co-enzymatic hydrolysis with nattokinase and serrapeptase, and finally co-enzymatic hydrolysis with aminopeptidase and transglutaminase. The present invention adopts a special method to prepare the peptide extracted from the gelatinous layer of sand jellyfish and the bamboo yellow-Grifola frondosa composite bacterial peptide, and the combined use achieves good biological activity, stability, and absorption utilization rate, overcomes the defects of existing bone repair and growth drugs, and effectively promotes bone repair and growth.

The invention belongs to the technical field of nattokinase, and particularly discloses nattokinase powder based on superfine grinding of natto as well as a preparation method and application thereof, and the preparation method comprises the following steps: S1, uniformly mixing natto and a protective agent, spreading the mixture in a container, and pre-freezing for 2-24 hours to obtain pretreated natto; s2, transferring the natto into a freeze dryer, and sequentially performing normal-pressure cooling, vacuum heating and normal-pressure heating treatment to obtain freeze-dried natto; s3, transferring the anti-caking agent and the freeze-dried natto into a crusher, crushing and sieving to obtain crude natto powder; and S4, transferring the crude natto powder to a jet mill, and performing crushing, sieving and packaging to obtain the nattokinase powder. According to the nattokinase powder based on superfine grinding of natto as well as the preparation method and the application of the nattokinase powder, the specific surface area is increased, so that surface active groups are exposed, the adsorbability and the solubility are enhanced, the eating feeling of a beverage is improved, and the absorption rate of nattokinase is further increased after the beverage is eaten.

The invention belongs to the technical field of novel application of nattokinase, and provides application of nattokinase in preparation of a medicine for preventing and / or treating Parkinson's disease. According to the invention, an MPTP / MPP + induced Parkinson's disease (PD) in-vivo and in-vitro model is established, and a neuroprotection mechanism of nattokinase is disclosed by adopting a systematic experiment method. Results show that the nattokinase can significantly improve dyskinesia and intestinal dysfunction of MPTP-induced PD mice; the damage of dopaminergic neurons of the middle brain is effectively relieved, and the density of tyrosine hydroxylase positive neurons is maintained; the expression of TLR4 and NLRP3 inflammasome pathways and related proinflammatory cytokines is inhibited, and the neuroinflammatory response is relieved; pINK1 / Parkin expression is up-regulated, mitochondrial autophagy is activated, and damaged neurons are cleared. Direct association between nattokinase and Parkinson's disease prevention and treatment is established for the first time, an innovative solution is provided for developing a novel anti-Parkinson's disease medicine with dual effects of motor function improvement and neuroprotection, and the application has important development value in the field of treatment of neurodegenerative diseases.

The application discloses a kind of mixed fermentation of coix seed rice natto and its preparation method, belong to natto fermentation technical field.The preparation method includes that clean soybean is soaked with coix seed saccharification liquid, NaCl is added after soaking and sterilized, Bacillus subtilis and bifidobacterium are inoculated in the material obtained by sterilization, and mixed fermentation is carried out.The application first proposes the method for preparing natto by soaking soybean with coix seed saccharification liquid and mixed fermentation of Bacillus subtilis and bifidobacterium, and the method for preparing natto by soaking soybean with coix seed saccharification liquid and mixed fermentation of Bacillus subtilis and bifidobacterium, coix seed saccharification liquid soaking and double bacteria fermentation promote the number of viable bacteria during natto fermentation and natto kinase enzyme activity, further affect the composition and content of active substance and flavor substance in subsequent fermentation process.The application improves the flavor of natto by process optimization, increases its nutrition, and obtains a novel unique, taste rich, flavor good high natto kinase activity flavor natto.

The present invention relates to a pharmaceutical composition for reducing uric acid and a preparation method thereof, belonging to the field of biotechnology. The pharmaceutical composition contains earthworm peptides, fungal fermentation products, fenugreek seed extract, chicory extract, and plantain extract; the earthworm peptides contain products obtained by successively subjecting earthworms to hierarchical enzymatic hydrolysis with trypsin, lumbrokinase, and nattokinase; the fungal fermentation products contain products obtained by jointly fermenting the pulp of cornel and smilax glabra rhizome with bacillus subtilis and bifidobacterium adolescentis; the fenugreek seed extract contains products obtained by successively subjecting fenugreek seed kernels to hierarchical enzymatic hydrolysis with ficin, trypsin, and papain; the chicory extract is an ethanol percolation extract of chicory roots; the plantain extract is an ethanol percolation extract of plantain. The combined use of each component has good uric acid reducing and anti-inflammatory effects, can quickly and efficiently relieve inflammation and reduce the blood uric acid content, and is safe and reliable.

The invention belongs to the technical field of biological materials, and provides a preparation method and application of a Prussian blue loaded nattokinase nanocomposite, RPB-NK nanoparticles are prepared by establishing a mouse radiation-induced pulmonary fibrosis model, compounding Prussian blue and nattokinase and then coating, and the RPB-NK nanoparticles are applied to prevention and treatment of radiation-induced pulmonary fibrosis diseases. The treatment effect on radiation-induced pulmonary fibrosis is evaluated; results show that the RPB-NK nanoparticles have a treatment effect on radiation-induced pulmonary fibrosis and can relieve the degree of radiation-induced pulmonary fibrosis so as to achieve the purpose of preventing and treating radiation-induced pulmonary fibrosis.

The application discloses a kind of blood thrombus biological pharmaceutical compositions containing peptide and preparation method thereof, belong to the technical field of biological medicine containing peptide.The composition includes earthworm protein, natto kinase, inulin-soybean peptide covalent modifier, peanut peptide and sodium alginate-chitosan double embedding adjuvant.Preparation method is through the construction inulin-soybean peptide covalent modifier and sodium alginate-chitosan double embedding process, solve the technical problems such as low oral bioavailability of polypeptide thrombolytic component, difficulty in dissolving old thrombus, high risk of bleeding, realize the synergistic effect of thrombolysis, vascular repair and safety.

The application belongs to the technical field of natto kinase micro-pellet preparation, and particularly relates to enteric-soluble sustained-release natto kinase micro-pellet particles, a one-step forming method thereof and application. The enteric-soluble sustained-release natto kinase micro-pellet particles are prepared by a method comprising the following steps: spraying and drying natto kinase fermentation liquor containing auxiliary materials, a sustained-release agent solution and an enteric-soluble agent solution to obtain enteric-soluble sustained-release natto kinase micro-pellet particles, wherein the dry matter content of the natto kinase fermentation liquor containing auxiliary materials is 30-60 wt%, the content of the sustained-release agent in the sustained-release agent solution is 0.1-30 wt%, and the content of the enteric-soluble agent in the enteric-soluble agent solution is 0.1-30 wt%. The one-step forming method of the application realizes one-step drying, forming and coating of the natto kinase fermentation liquor containing auxiliary materials, and improves the preparation efficiency of the enteric-soluble sustained-release natto kinase micro-pellet particles.

The present invention relates to a polypeptide derived from natural tridegin by structural modification, which may serve as an inhibitor of plasma coagulation factor XIIIa, and to pharmaceutical compositions comprising such polypeptide. The present invention further refers to such polypeptide or pharmaceutical composition for use in a method for treating or preventing a thrombotic event in a patient. Moreover, the invention relates to an endoprosthesis or an enzyme-linked immunosorbent assay (ELISA) plate coated with a polypeptide of the present invention. Furthermore, the invention also encompasses a recombinant fusion protein comprising this polypeptide linked to a fibrinolytic enzyme such as streptokinase, nattokinase or tPAs via a flexible peptide linker containing a thrombin-cleavable site. This construct allows selective inhibition of FXIIIa within a thrombus, while enabling thrombin-triggered release of the fibrinolytic enzyme to induce localized fibrinolysis, thereby achieving targeted dual-action thrombolytic therapy.

The present application belongs to the field of fermented food preparation, and relates to a process for preparing dried natto products by a micro-expansion method, comprising the following steps: pretreating beans to obtain fermented natto; pre-drying the fermented natto, and then micro-expansion drying to obtain dried natto products; the micro-expansion drying is: pressurizing under the condition that the expansion temperature is 50-70 DEG C, and stopping for a period of time; instant expansion under the condition of high pressure difference; continuing vacuum drying; the vacuum temperature is 50-70 DEG C, and the micro-expansion pressure difference is 0.5-0.6 MPa; wherein, the pre-drying comprises freeze-thaw treatment and hot air drying; the condition of the hot air drying is: drying under the condition that the temperature is 40-60 DEG C, and the water content of the dried natto is 15%-25%. The drying process adopts freeze-thaw treatment, hot air drying and micro-expansion drying, and the three processes cooperatively constitute a set of drying process, which can ensure the activity of natto kinase and the expansion result, so as to improve the quality of natto.

This invention relates to a blackcurrant and orange juice mixed soft drink, belonging to the field of beverage technology. The mixed soft drink contains blackcurrant extract, orange juice, white hyacinth bean seed extract, mogrosides, steviol glycosides, etc. The blackcurrant extract is a product with a lower than 5kDa obtained by enzymatic hydrolysis of blackcurrants with hemicellulase and pectinase, followed by sequential enzymatic hydrolysis with nattokinase, fig protease, papain, and glucokinase. The white hyacinth bean seed extract is a product with a lower than 5kDa obtained by trypsin hydrolysis of white hyacinth bean seeds, followed by co-fermentation with Propionibacterium finigense, Lactobacillus brevis, and Bifidobacterium longum. The blackcurrant extract and white hyacinth bean seed extract have excellent anti-fatigue, antioxidant, and immune-enhancing effects, making the mixed soft drink both delicious and healthy.

The invention relates to the technical field of meal replacement powder, provides meal replacement powder for improving intestinal environment and a preparation method, and aims to provide comprehensive nutrition and promote intestinal health through raw material combination in scientific proportion. The meal replacement powder consists of a component A (hemp seed protein powder and quinoa protein isolate), a component B (semen plantaginis husk powder and cactus fruit powder), a component C (oenothera biennis oil powder and sea buckthorn seed oil powder), a component D (agave syrup powder) and a component E (nattokinase and bacillus coagulans viable powder). The preparation method comprises the steps of low-temperature superfine grinding, multi-enzyme composite enzymolysis, embedding of probiotics and enzymes, fat microencapsulation, mixing and grinding, drying and packaging and the like. The meal replacement powder capable of improving the intestinal environment has positive effects in the aspects of promoting small intestine peristalsis, improving constipation, increasing the number of beneficial bacteria, reducing the number of harmful bacteria and maintaining intestinal microecological balance.