76 results about "Nattokinase" patented technology

The invention discloses a preparation method and application of yak blood peptide with anti-hypoxia and anti-fatigue functions. The method comprises the steps of raw material pretreatment, composite enzymolysis (adopting a mixture of enzyme digestion modified lumbrukinase and nattokinase), enzyme deactivation, separation and purification, ferrous chelation, Maillard reaction, concentration and drying and the like. The enzymolysis efficiency and the active peptide yield are remarkably improved through ultrasonic treatment and a composite enzymolysis technology, the stability and the anti-hypoxia capacity of peptide fragments are enhanced through ferrous chelation, and the antioxidant activity and the anti-fatigue activity are further improved through the Maillard reaction. Experiments show that the yak blood peptide prepared by the method can significantly improve the antioxidant enzyme activity, prolong the normal-pressure hypoxia survival time, prolong the swimming exhaustion time and significantly reduce the MDA content of brain tissue and myocardial tissue. The obtained product can be applied to anti-hypoxia and anti-fatigue medicines or health-care foods, and has the advantages of high process efficiency, remarkable activity, high safety and the like.

The invention belongs to the technical field of health-care food, and particularly relates to a sacha inchi oil-based gel candy with functions of reducing hypertension, hyperglycemia and hyperlipidemia and a preparation method thereof, and the sacha inchi oil-based gel candy comprises the following components: 1) a main component: 30-70% of sacha inchi oil; 2) a blood fat reducing synergistic component which is one or a combination of more of 0.5%-5% of phytosterol, 0.1%-2% of a red yeast rice extract and 0.01%-0.5% of sugarcane octacosanol; 3) a hypoglycemic synergistic component, which comprises one or a combination of more of 5%-20% of inulin / fructo-oligosaccharide, 0.5%-5% of a bitter gourd extract, 0.5%-5% of a mulberry leaf extract and 10-200 [mu] g / grain of chromium; 4) an antihypertensive synergistic component, wherein the antihypertensive synergistic component is one or a combination of more of 500-5000FU / grain of nattokinase, 0.5-5% of a celery seed extract and 1-8% of a hawthorn extract; through scientific compounding, the sacha inchi oil and various synergistic components exert a combined effect, and meanwhile, auxiliary regulation is carried out on blood sugar, blood fat and blood pressure; the main components are derived from natural plant extracts or functional grease, so that the health-care product is high in safety and suitable for daily health care.

The invention relates to the technical field of traditional Chinese medicine health-care products, and particularly discloses an earthworm protein peptide composition for improving vascular embolism and a preparation method thereof.The earthworm protein peptide composition is prepared from, by weight, 28-34 parts of earthworm protein peptide, 20-26 parts of active pharmaceutical ingredients, 1-5 parts of ginkgo biloba extract, 12-15 parts of corn oligopeptide powder and 4-8 parts of auxiliary materials; lumbricus protein peptide, active pharmaceutical ingredients, ginkgo biloba extract and corn oligopeptide powder are used as raw materials, a solubilizing component is added in the preparation process, the solubilizing component is prepared by mixing functional extract, nattokinase and tween-80 according to a certain proportion, the functional extract is prepared by coating grape seed extract with hydroxypropyl-beta-cyclodextrin, and the solubilizing component is prepared by mixing the functional extract, the nattokinase and the tween-80 according to a certain proportion. Through the synergistic effect of the components, the antithrombotic effect of the earthworm protein peptide composition is synergistically enhanced.

The invention provides natto as well as a preparation method, health-care food and application thereof. The preparation method comprises the following steps: soaking soybeans; sterilizing the soaked soybeans; inoculating the sterilized soybeans with bacillus natto for first-stage fermentation, and then adding lactose into a fermentation system for second-stage fermentation to obtain natto; wherein the addition amount of the lactose is that 1-10% of the lactose is added on the basis that the mass of the dry beans is 100%. According to the method, special odor of traditional natto is removed, and the prepared natto powder has good nattokinase activity and a remarkable blood sugar reducing effect.

The invention discloses a nattokinase product for preventing and / or treating atherosclerosis and application of the nattokinase product. Cardiovascular diseases become the primary reason of human death, and the vascular plaque problem caused by atherosclerosis is always a difficult problem to be solved urgently. Blood vessel stenosis caused by plaques is a main cause of cerebral arterial thrombosis, and traditional medicines and operations face the problems of low compliance, side effect risk, indication limitation and the like. The invention provides nattokinase or a product thereof for high-risk groups with cardiovascular diseases, and the nattokinase or the product thereof is preferably in a non-drug form. As a precise nutrition intervention means, nattokinase is effective in plaque progress inhibition, unique in effect, safe to eat, convenient, easy to implement and wide in applicable crowd. The invention provides a new thought and method for management of vascular plaques and prevention and treatment of cardiovascular diseases.

The invention relates to the technical field of microbial fermentation engineering, and particularly discloses natto red yeast rice herb bean flour and a preparation method thereof. The preparation method comprises the following steps: respectively activating specifically preserved bacillus natto and monascus to obtain secondary seed solutions; inoculating the two seed solutions into a solid-state fermentation culture medium for co-fermentation; and inactivating, drying and crushing the fermentation product to obtain a finished product. The solid-state fermentation culture medium is prepared by compounding a bean matrix (soybeans, black beans or chickpeas) and herb tea components (roses, lotus leaves, chrysanthemums, mulberry leaves, haws, cassia seeds and the like). Through the synergistic effect of the bacillus natto and the monascus, nattokinase and monacolin K are efficiently enriched in a single fermentation system at the same time, conversion of soy isoflavone to a high-bioavailability aglycone type is remarkably promoted, and the obtained product has multiple health-care functions of thrombolysis, lipid reduction, efficient absorption and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to application of a nattokinase preparation in preparation of a medicine for preventing or treating sepsis. The nattokinase preparation comprises an electrostatic complex prepared from nattokinase and an anionic compound, the mass ratio of the nattokinase to the anionic compound ranges from 1: 1 to 1: 32, and the anionic compound is selected from one or two of polysialic acid or hyaluronic acid. Research results show that the nattokinase preparation not only can significantly improve the survival rate and weight of sepsis mice, but also can effectively stimulate the organism to generate potent and lasting immune memory response, and enhance the risk of the mice to resist secondary or more lipopolysaccharide attacks.

This invention discloses a small-molecule soybean peptide, its preparation method, and its applications. The preparation method includes: mixing soybean protein isolate with water and then performing pH shift pretreatment, deep eutectic solvent pretreatment, and pulsed electric field pretreatment; subsequently, enzymatic hydrolysis, enzyme inactivation, centrifugation, and collection of the supernatant; then, performing a two-step fractional alcohol precipitation treatment on the supernatant, collecting the precipitate, reconstituteing, and freeze-drying to obtain the small-molecule soybean peptide. This soybean peptide has a molecular weight <1 kDa and a component content of over 94%, and can be used as a nitrogen source to replace soybean peptone in Bacillus subtilis fermentation medium. This small-molecule soybean peptide has a good yield-promoting effect on at least five Bacillus subtilis strains and can be used to promote the production of nattokinase by Bacillus subtilis biofilm fermentation, with the highest nattokinase activity reaching approximately 14800 U / mL.

The invention relates to a composition for preventing cardiovascular and cerebrovascular diseases and a preparation method thereof, and belongs to the technical field of health food. The composition for preventing cardiovascular and cerebrovascular diseases is prepared from the following components in parts by weight: 20 to 100 parts of dihydroquercetin, 100 to 300 parts of red yeast rice, 50 to 150 parts of nattokinase, 50 to 200 parts of earthworm protein, 100 to 300 parts of hawthorn fruit extract, 100 to 300 parts of radix puerariae extract and 100 to 300 parts of sea-buckthorn extract. The composition prepared by the invention has the characteristics of lowering lipid, dissolving thrombus and protecting heart and blood vessels.

The invention belongs to the technical field of nattokinase, and particularly discloses nattokinase powder based on superfine grinding of natto as well as a preparation method and application thereof, and the preparation method comprises the following steps: S1, uniformly mixing natto and a protective agent, spreading the mixture in a container, and pre-freezing for 2-24 hours to obtain pretreated natto; s2, transferring the natto into a freeze dryer, and sequentially performing normal-pressure cooling, vacuum heating and normal-pressure heating treatment to obtain freeze-dried natto; s3, transferring the anti-caking agent and the freeze-dried natto into a crusher, crushing and sieving to obtain crude natto powder; and S4, transferring the crude natto powder to a jet mill, and performing crushing, sieving and packaging to obtain the nattokinase powder. According to the nattokinase powder based on superfine grinding of natto as well as the preparation method and the application of the nattokinase powder, the specific surface area is increased, so that surface active groups are exposed, the adsorbability and the solubility are enhanced, the eating feeling of a beverage is improved, and the absorption rate of nattokinase is further increased after the beverage is eaten.

The invention belongs to the technical field of biological materials, and provides a preparation method and application of a Prussian blue loaded nattokinase nanocomposite, RPB-NK nanoparticles are prepared by establishing a mouse radiation-induced pulmonary fibrosis model, compounding Prussian blue and nattokinase and then coating, and the RPB-NK nanoparticles are applied to prevention and treatment of radiation-induced pulmonary fibrosis diseases. The treatment effect on radiation-induced pulmonary fibrosis is evaluated; results show that the RPB-NK nanoparticles have a treatment effect on radiation-induced pulmonary fibrosis and can relieve the degree of radiation-induced pulmonary fibrosis so as to achieve the purpose of preventing and treating radiation-induced pulmonary fibrosis.

The application discloses a kind of blood thrombus biological pharmaceutical compositions containing peptide and preparation method thereof, belong to the technical field of biological medicine containing peptide.The composition includes earthworm protein, natto kinase, inulin-soybean peptide covalent modifier, peanut peptide and sodium alginate-chitosan double embedding adjuvant.Preparation method is through the construction inulin-soybean peptide covalent modifier and sodium alginate-chitosan double embedding process, solve the technical problems such as low oral bioavailability of polypeptide thrombolytic component, difficulty in dissolving old thrombus, high risk of bleeding, realize the synergistic effect of thrombolysis, vascular repair and safety.

The application belongs to the technical field of natto kinase micro-pellet preparation, and particularly relates to enteric-soluble sustained-release natto kinase micro-pellet particles, a one-step forming method thereof and application. The enteric-soluble sustained-release natto kinase micro-pellet particles are prepared by a method comprising the following steps: spraying and drying natto kinase fermentation liquor containing auxiliary materials, a sustained-release agent solution and an enteric-soluble agent solution to obtain enteric-soluble sustained-release natto kinase micro-pellet particles, wherein the dry matter content of the natto kinase fermentation liquor containing auxiliary materials is 30-60 wt%, the content of the sustained-release agent in the sustained-release agent solution is 0.1-30 wt%, and the content of the enteric-soluble agent in the enteric-soluble agent solution is 0.1-30 wt%. The one-step forming method of the application realizes one-step drying, forming and coating of the natto kinase fermentation liquor containing auxiliary materials, and improves the preparation efficiency of the enteric-soluble sustained-release natto kinase micro-pellet particles.

The present invention relates to a polypeptide derived from natural tridegin by structural modification, which may serve as an inhibitor of plasma coagulation factor XIIIa, and to pharmaceutical compositions comprising such polypeptide. The present invention further refers to such polypeptide or pharmaceutical composition for use in a method for treating or preventing a thrombotic event in a patient. Moreover, the invention relates to an endoprosthesis or an enzyme-linked immunosorbent assay (ELISA) plate coated with a polypeptide of the present invention. Furthermore, the invention also encompasses a recombinant fusion protein comprising this polypeptide linked to a fibrinolytic enzyme such as streptokinase, nattokinase or tPAs via a flexible peptide linker containing a thrombin-cleavable site. This construct allows selective inhibition of FXIIIa within a thrombus, while enabling thrombin-triggered release of the fibrinolytic enzyme to induce localized fibrinolysis, thereby achieving targeted dual-action thrombolytic therapy.

The present application belongs to the field of fermented food preparation, and relates to a process for preparing dried natto products by a micro-expansion method, comprising the following steps: pretreating beans to obtain fermented natto; pre-drying the fermented natto, and then micro-expansion drying to obtain dried natto products; the micro-expansion drying is: pressurizing under the condition that the expansion temperature is 50-70 DEG C, and stopping for a period of time; instant expansion under the condition of high pressure difference; continuing vacuum drying; the vacuum temperature is 50-70 DEG C, and the micro-expansion pressure difference is 0.5-0.6 MPa; wherein, the pre-drying comprises freeze-thaw treatment and hot air drying; the condition of the hot air drying is: drying under the condition that the temperature is 40-60 DEG C, and the water content of the dried natto is 15%-25%. The drying process adopts freeze-thaw treatment, hot air drying and micro-expansion drying, and the three processes cooperatively constitute a set of drying process, which can ensure the activity of natto kinase and the expansion result, so as to improve the quality of natto.

This invention relates to a blackcurrant and orange juice mixed soft drink, belonging to the field of beverage technology. The mixed soft drink contains blackcurrant extract, orange juice, white hyacinth bean seed extract, mogrosides, steviol glycosides, etc. The blackcurrant extract is a product with a lower than 5kDa obtained by enzymatic hydrolysis of blackcurrants with hemicellulase and pectinase, followed by sequential enzymatic hydrolysis with nattokinase, fig protease, papain, and glucokinase. The white hyacinth bean seed extract is a product with a lower than 5kDa obtained by trypsin hydrolysis of white hyacinth bean seeds, followed by co-fermentation with Propionibacterium finigense, Lactobacillus brevis, and Bifidobacterium longum. The blackcurrant extract and white hyacinth bean seed extract have excellent anti-fatigue, antioxidant, and immune-enhancing effects, making the mixed soft drink both delicious and healthy.

The invention relates to the technical field of meal replacement powder, provides meal replacement powder for improving intestinal environment and a preparation method, and aims to provide comprehensive nutrition and promote intestinal health through raw material combination in scientific proportion. The meal replacement powder consists of a component A (hemp seed protein powder and quinoa protein isolate), a component B (semen plantaginis husk powder and cactus fruit powder), a component C (oenothera biennis oil powder and sea buckthorn seed oil powder), a component D (agave syrup powder) and a component E (nattokinase and bacillus coagulans viable powder). The preparation method comprises the steps of low-temperature superfine grinding, multi-enzyme composite enzymolysis, embedding of probiotics and enzymes, fat microencapsulation, mixing and grinding, drying and packaging and the like. The meal replacement powder capable of improving the intestinal environment has positive effects in the aspects of promoting small intestine peristalsis, improving constipation, increasing the number of beneficial bacteria, reducing the number of harmful bacteria and maintaining intestinal microecological balance.

The application discloses a sports nutrition food composition for blood vessel health and post-exercise recovery. Each 40g contains soybean protein isolate, whey protein, resistant dextrin, inulin, ginkgo biloba extract, hawthorn extract, grape seed extract, sea buckthorn peptide, natto powder (containing natto kinase), coenzyme Q10, natural vitamin E, vitamin C, bacillus subtilis DE111 and mineral probiotic premix. The application realizes blood vessel cleaning and post-exercise recovery through the synergy of multiple pathways such as direct thrombolytic effect of natto kinase, anti-platelet aggregation of ginkgo biloba / hawthorn / grape seed, protection of blood vessel endothelium by coenzyme Q10 / VE / VC, and regulation of intestinal tract by bacillus subtilis DE111. In-vitro thrombolysis experiment shows that the composition has clear fibrinolytic activity. All components meet the national standard of sports nutrition food, are safe and compliant, and are suitable for people with thrombosis risk and people who need blood vessel repair after high-intensity exercise.

In the field of medical supplies and food / drug storage technology, we provide a drug storage device for maintaining nattokinase activity. The solution includes a box body 1 and a screw-fitted box cover 2. The box body contains a main box 11 for storing capsules and a micro-perforated desiccant inner tray chamber 12, which physically isolates the capsules and keeps them fresh. The key feature is the activity detection module 21 integrated into the box cover. This module is equipped with a dedicated capsule detection chamber, and uses a built-in needle sampling mechanism to drip the capsule contents onto a specific colorimetric substrate test strip at the bottom. An optical sensor reads the RGB values ​​of the color reaction, and after processing by an MCU controller, the activity results are output via a display mechanism. This device integrates in situ, rapid, quantitative detection of nattokinase capsule activity with intelligent storage, providing simple operation and intuitive, reliable results. It solves the problems of conventional technology, which make it difficult to easily quantitatively detect nattokinase capsule activity and which are susceptible to moisture inactivation during storage.

This invention belongs to the field of food science and technology, specifically relating to a composition for cardiovascular protection and its preparation method. The composition includes freeze-dried natto powder, milk protein peptide powder, dihydromyricetin, and functional red yeast rice powder. The freeze-dried natto powder has a nattokinase content of 3000 FU / g, 5000 FU / g, 10000 FU / g, or 20000 FU / g. The milk protein peptide powder has a total VPP and IPP content ≥5.0%, and the milk protein peptide powder has an ACE inhibition rate ≥63%. This invention addresses the limitations of existing cardiovascular health foods that rely on single-component efficacy and cannot achieve multi-target, comprehensive protection. It employs a scientific combination of four natural active ingredients—freeze-dried natto powder, milk protein peptide powder, dihydromyricetin, and functional red yeast rice powder—to achieve synergistic effects across multiple targets.

The invention discloses a preparation method of high-protein natto noodles, and belongs to the technical field of food processing. The natto powder is embedded by gelatin, so that the stability of active ingredients in the natto powder in the processing process is effectively protected, and the retention rate of nutritional ingredients is increased; through a unique sandwich type composite calendering process, dough sheets containing natto powder are placed in the middle layer, and common dough sheets cover the two sides, so that effective distribution of active ingredients of natto in the noodles is ensured, and the influence of the external environment on natto flavor substances is effectively blocked, so that the finished product noodles not only can retain specific functional ingredients of natto, but also can be used for preparing the natto noodles. The situation that the eating palatability is affected due to the fact that the characteristic flavor of natto is too prominent is avoided, the survival rate of nattokinase in the noodle cooking process is increased, and the nattokinase activity protection rate reaches 20.5%.

The invention provides a soda water beverage added with nattokinase and a preparation method and application thereof, the soda water beverage added with nattokinase has a pH value of 7.8-8.2 and a carbonation degree of 0.8-1.2 g / L, and contains nattokinase, and the activity of the nattokinase is greater than or equal to 10,000 FU / L. The soda water beverage added with the nattokinase is applied to preparation of products for regulating PCSK9 activity, reducing the low density lipoprotein cholesterol LDL-C level in blood or preventing / assisting in managing obesity and related metabolic syndromes. The product is a beverage, a health food or a dietary supplement. The nattokinase can be used for specifically hydrolyzing the Ser153-Gly154 site of the PCSK9 protein, and the Ser153-Gly154 site of the PCSK9 protein can be specifically hydrolyzed. According to the beverage, the activity of nattokinase is protected by optimizing pH and a carbonation system, the intestinal absorption rate of the nattokinase is improved, and the function of PCSK9 is inhibited in a targeted manner so as to regulate blood lipid metabolism. Compared with an existing oral enzyme preparation, the oral enzyme preparation has high efficiency, stability and consumption convenience, and an innovative diet intervention scheme is provided for obesity and related metabolic diseases.

Capsule structure (C) with a red yeast compound, comprising: - a capsule body (10) comprising a first capsule shell (11) and a second capsule shell (12), wherein the first capsule shell (11) and the second capsule shell (12) are placed on top of each other in such a way that they together enclose a closed receiving space (13); and - several functional particles (20) arranged in the receiving space (13) of the capsule body (10), wherein the composition of the functional particles (20) includes red yeast (21), nattokinase (22) and coenzyme Q10 (23).

The application provides a high-salt-tolerant Bacillus subtilis capable of producing protease and nattokinase and an application thereof, the Bacillus subtilis is named as Bacillus subtilis 3-1, is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20252875, and the 16S rRNA nucleotide sequence is shown as SEQ ID NO: 1. The Bacillus subtilis of the application can be directly applied to a high-salt fermentation system due to excellent high-salt-tolerant performance, and solves the industrial problem that the activity of a conventional strain is inhibited; due to the protease production property, can be used for efficiently degrading raw material protein in the field of food fermentation, promoting flavor formation and shortening the production cycle; the nattokinase production property makes it have outstanding value in the field of functional food and preparation, and can be used for developing a fermented product with health care functions of dissolving thrombus, and improving the health added value of the product. The strain provides a core microbial resource for quality improvement and functional development of high-salt fermented food.

The invention provides oral cyclodextrin inclusion complex formulations in which inclusion complexes of borneol and trans-cinnamaldehyde are provided together with nattokinase and with an enzyme having a cyclodextrin-degrading activity capable of digesting cyclodextrins, so that upon delivery of the formulation to a target tissue the CD- degrading enzyme is activated and releases the borneol and trans-cinnamaldehyde from the cyclodextrins. These cyclodextrin inclusion complex formulations may be used for treating atheromatous plaque and related diseases.

This invention discloses a production process for highly active nattokinase. The process includes the preparation of seed liquid, raw material processing and preparation, liquid fermentation, and spray drying. In this process, the soybean milk is treated with 0.05%–4.5% neutral protease for enzymatic hydrolysis, which effectively improves the enzyme activity of nattokinase. The spray drying process, with the addition of 20%–35% maltodextrin and inulin as protective agents, offers advantages such as minimal enzyme activity loss at high temperatures, low energy consumption, and high spray drying efficiency. The nattokinase spray-dried powder produced by this process has high activity and high viable cell count, with an enzyme activity reaching 62900 IU / g and a colony count of 7.4*10⁻⁶. 11 CFU / g or higher. Compared with other fermentation methods, this method has simpler raw material processing, lower cost, lower energy consumption, less pollution, higher production efficiency, and simpler process. Therefore, this invention has broad market prospects and industrial production application value.

The invention provides a method for improving nattokinase activity, which comprises the following steps: adding a bean natural extract into a basic seed culture medium containing a carbon source, a nitrogen source and a metal ion source to obtain a target seed culture medium, inoculating bacillus natto freeze-dried powder, and culturing to obtain a thallus activation solution; the method comprises the following steps: sequentially carrying out ultrahigh pressure treatment and grinding treatment on bean raw materials to obtain raw material slurry, mixing the raw material slurry with a carbon source, a nitrogen source and a metal ion source, and sterilizing to obtain a fermentation medium; inoculating the thallus activation solution to a fermentation culture medium for segmented fermentation; and sequentially carrying out low-temperature centrifugation, filtration purification and low-temperature precipitation treatment on the fermentation product, redissolving the obtained nattokinase precipitate with a solution, and then carrying out physical freeze-drying treatment. The bean natural extract is used for promoting thallus activation, ultrahigh pressure treatment is used for releasing nutrient substances, fermentation parameters are regulated and controlled in stages to promote high-efficiency synthesis of nattokinase, low-temperature purification is used for retaining enzyme activity, and finally high-activity nattokinase is obtained.

This invention provides a fermentation apparatus for high-yielding nattokinase strains. [Solution] The system includes a fermentation tank 1, a support frame 2 fitted to the outside of the fermentation tank, a bottom plate 3 fixedly connected to the lower end of the support frame, a material supply pipe fixedly connected to the upper end of the fermentation tank, an aeration pipe 6 fixedly connected to the lower end of the fermentation tank, and an aeration assembly rotatably connected to one end of the aeration pipe. By coordinating the rotating cavity 7 of the aeration assembly with a plurality of branched aeration members 8, sterile gas is converted into fine bubbles by passing it through the fine pores of the microbubble aeration head, thereby significantly increasing the gas-liquid contact area. At the same time, the reaction force generated during aeration drives the rotating cavity and branched aeration members to rotate synchronously, so that the fine bubbles diffuse uniformly into each region of the internal cavity of the fermentation tank.

The invention discloses a kale extract composition with the effects of reducing dietary fat absorption and assisting in reducing three highs and application of the kale extract composition, and aims to solve the problems that an existing dietary therapy product is weak in fat absorption inhibition, poor in three-high synergistic regulation, low in kale activity utilization rate and insufficient in palatability and stability. The composition is prepared by taking kale extract (which is prepared by steam fixation at 105 DEG C, ultrasonic extraction with 80% ethanol, purification with D101 macroporous resin, and low-temperature spray drying at 180 DEG C / 70 DEG C, and total dietary fiber is more than or equal to 30%, total polyphenol is more than or equal to 5%, and phytosterol is more than or equal to 2%) as a core, compounding nattokinase, fructo-oligosaccharide, vitamin C and stevioside (the mass ratio is 60%-80%: 5%-10%: 10%-20%: 1%-5%: 0.1%-0.5%), and uniformly mixing the nattokinase, the fructo-oligosaccharide, the vitamin C and the stevioside. The food can be prepared into forms of capsules, meal replacement powder, solid beverages and the like, can reduce 25%-30% of dietary fat absorption, can assist in reducing 12%-15% of total cholesterol, 6-8 mmHg of systolic pressure and 0.4-0.6 mmo l / L of fasting blood-glucose, has activity loss of less than or equal to 8% after being stored at normal temperature for 6 months, has no gastrointestinal discomfort, is suitable for people needing metabolism management, and has efficacy, palatability and practicability.

The strain BS-14 can convert the nutrients in the solid culture into nutrients absorbed and utilized by the bacillus subtilis, and the microenvironment generated by the fermentation of the strain BS-14 is also beneficial to promote the fermentation of MQ013-26, so that the enzyme activity concentration of the natto kinase is further improved. The strain BS-14 can further decompose phospholipid substances (phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol) and fat, so that the spoilage odor of the freeze-dried powder can be further improved, and the shelf life can be prolonged. The strain BS-14 can further generate active peptides against pathogenic microorganisms, and has significant bacteriostatic activity, so that the quality safety of the freeze-dried powder is improved.