116results about How to "Reduce purification costs" patented technology

The invention provides a novel human-derived II-type collagen COLII-14K-H and a coding gene thereof, the coding gene is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO.2. The invention further provides a preparation method of the novel human-derived II-type collagen COLII-14K-H. The invention also provides a preparation method of the human-derived II-type collagen COLII-14K-H and application of the human-derived II-type collagen COLII-14K-H in preparation of cartilage repair drugs, skin repair drugs and skin care products. The inventor-derived II-type collagen can obviously improve the gene expression level of the collagen, so that the content of the collagen in human dermal fibroblasts is improved, and the skin aging is delayed; meanwhile, the protein fragment can show a remarkable skin repairing effect at low concentration, provides a new treatment choice for skin repairing, and can be widely applied to the fields of skin repairing and nursing.

The invention discloses a light adsorption-desorption-resistant adsorption tower, a coating process, a helium purification system and a helium purification method, and mainly aims to solve the problem that in the prior art of natural gas helium purification, adsorption tower equipment is aged and corroded, and the purity stability of a helium product is influenced by adsorption and desorption formed by the adsorption tower. The light adsorption tower resistant to adsorption and desorption is provided, the service life of adsorption equipment is prolonged while the purity stability of a helium product is improved, and meanwhile the cost of helium purification through adsorption is reduced through the structural design of a helium purification system.

The invention relates to a codon optimization method for improving the protein expression quantity of a target gene in a plant, and belongs to the technical field of gene engineering. The invention provides a codon optimization method for improving the protein expression quantity of a target gene in a plant, which comprises the step of replacing the third basic group of a codon of a target protein coding sequence with C or G to obtain an optimized target protein coding sequence. The method has effectiveness and universality in plants, and research shows that after codon optimization of the method, the protein expression quantity of target genes in a plant protein transient expression system and a transgenic plant is increased by several times to dozens of times. According to the production application of the functional protein taking the plant as the bioreactor, the yield of the target protein can be increased, and the protein purification cost can be reduced; according to the application of the method in preparation of the transgenic plant, the expression quantity of the target gene can be increased, so that the target character of the transgenic plant is enhanced, and the screening cost of the high-expression transgenic plant is reduced.

The invention relates to the technical field of carbon nanomaterials, in particular to a single-walled carbon nanotube purification method. Introducing a solvent into the initial sample of the single-walled carbon nanotube: putting the single-walled carbon nanotube with the mass A into a container, adding the solvent with the mass B, and fully stirring to obtain a wet material; low-temperature oxidation in a strong oxidizing atmosphere: putting the wet single-walled carbon nanotube material into a reactor, continuously introducing inert gas, heating the reactor to a relatively low temperature T1, introducing strong oxidizing gas after the temperature is stable, and stopping introducing the strong oxidizing gas after reacting for a certain time; and acid treatment: dispersing the obtained sample into an acid solution, stirring for reaction, and then filtering and drying to obtain the purified single-walled carbon nanotube. According to the method, the purification efficiency, retention rate and purity of the single-walled carbon nanotubes are greatly improved.

A process for separating fillers from catalysts and gases in plastics in a fluidized bed catalytic pyrolysis process is described that converts waste plastics, polymers, and other waste materials to useful chemicals and fuel products, such as aromatics such as paraffins, olefins, and BTX.

The invention discloses recombinant human chemical fibronectin as well as a coding gene and application thereof. The method comprises the following steps: performing codon optimization on an amino acid sequence of recombinant human chemical fibronectin according to codon preference of pichia pastoris to obtain a coding gene nucleotide sequence SEQ ID NO.2, cloning the coding gene nucleotide sequence SEQ ID NO.2 between EcoRI and NotI restriction enzyme cutting sites of a pichia pastoris expression vector pPIC9K to obtain a recombinant expression vector pPIC9K-FNc, and transferring the recombinant expression vector pPIC9K-FNc into a P.pastoris GS115 expression host cell to obtain the recombinant human chemical fibronectin. Recombinant pichia pastoris P.pastoris GS115 / pPIC9K-FNc is obtained through genetic mycin G418 screening, then recombinant bacteria are subjected to fermentation culture and are subjected to induced expression by using methanol, and then the recombinant human chemical fibronectin is obtained after purification. The fibronectin produced by the method is large in molecular weight and has better biological activities such as wrinkle resistance and barrier repair.

The invention relates to the technical field of amine liquid purification, and discloses a thermal power flue gas carbon capture amine liquid purification system utilizing carbon dioxide purging, which is connected with a carbon capture system and comprises a resin desalination module respectively connected with a purification pipeline, a gas transmission pipeline, a liquid transmission pipeline and an alkali liquor box; valves are arranged on the purification pipeline, the gas conveying pipeline and the liquid conveying pipeline; the purification pipeline is connected with the filtering subsystem; the filtering subsystem is connected with an amine liquid pipeline; amine liquid of the carbon capture system is input into the filtering subsystem through an amine liquid pipeline; the gas pipeline is connected with a gas outlet of the carbon capture system, and the gas outlet is used for discharging CO2 product gas of the carbon capture system; a compressor is further connected between the air outlet and the air conveying pipeline; the liquid conveying pipeline is respectively connected with the circulating water tank and the desalted water tank. According to the invention, stable alkali liquor cleaning can be realized, and on the basis of ensuring the cleaning cleanliness of the alkali liquor, the amount of wastewater is greatly reduced, the purification period is shortened, the purification efficiency is improved, and the purification cost is reduced.

The invention provides an integrated device integrating organic solid waste treatment, metal recovery and carbon material preparation and a use method of the integrated device, and belongs to the field of environmental science and engineering. The problems that the utilization rate of organic solid waste heat energy is low, the recovery rate of metal-containing waste materials is low, and the carbon yield and quality of carbon materials prepared from agriculture and forestry biomass waste are low are solved. The integrated device comprises a feeding system, a solid waste heat treatment system, a steam utilization system, a tail gas purification and recovery system, a biomass carbonization system and a metal pyrolysis and recovery system, the solid waste heat treatment system comprises a pyrolysis gasification chamber and a high-temperature cracking chamber, and the high-temperature cracking chamber is divided into a left chamber and a right chamber; the steam utilization system comprises a high-pressure steam chamber, a low-pressure steam chamber and a hot water chamber, and the tail gas purification and recovery system comprises a cyclone dust collector, a spraying denitrification and denitration chamber, a water bath purification chamber, an electrostatic dust collector and a gas collector. The method is mainly used for organic solid waste treatment, metal recovery and carbon material preparation.

The invention provides a method for producing biogas by synergistically strengthening anaerobic digestion of organic solid waste through micro-aerobic pretreatment and iron ions, and belongs to the technical field of environmental engineering. According to the method, microaerobic pretreatment is carried out on the organic solid waste according to the type of the organic solid waste so as to improve the concentration of dissolved organic matters in the organic solid waste, then in the anaerobic digestion process, the concentration of re-added iron ions is determined according to the total COD in the organic solid waste and the concentration of original iron ions in a system, and the concentration of the dissolved organic matters in the organic solid waste is improved by adding the iron ions. And the biogas yield (namely the volume biogas yield of the organic solid waste, namely the biogas yield of the organic solid waste per unit volume) is synergistically improved. According to the method, the organic matter degradation rate and the biogas yield of anaerobic digestion of the organic solid waste are effectively increased, reduction and recycling treatment of the organic solid waste are achieved, generation of foul gases such as hydrogen sulfide in the anaerobic digestion process is greatly reduced, the biogas purification cost is reduced, the economic benefits of anaerobic digestion engineering are remarkably increased, and the method is suitable for popularization.

The invention discloses a purification device of tetramethylsilane and a recovery method of tetramethylsilane in a steel cylinder. The tetramethylsilane purification device comprises a vaporizer, an adsorption column, a filter, an intermediate tank and a product tank. The vaporizer is connected with the steel cylinder and used for vaporizing tetramethylsilane in the steel cylinder, and a plurality of sample outlets and sample inlets are formed in the steel cylinder; one end of the adsorption column is connected with the vaporizer, and the adsorption column is used for adsorbing gaseous impurities in tetramethylsilane; the filter is connected with the other end of the adsorption column and is used for filtering particulate impurities in tetramethylsilane; the intermediate tank is communicated with the filter, can be communicated with the steel cylinder and is used for temporarily storing filtered tetramethylsilane; and the product tank is communicated with the intermediate tank and is used for storing purified tetramethylsilane. The purification device disclosed by the invention is simple in structure, and can be connected through a simple pipeline, so that the recovery of raw materials in the steel cylinder and the flushing and replacement of the steel cylinder can be completed on a production site, the production efficiency is improved, and the purification cost is reduced.

The utility model discloses a Fenton oxidation reaction device which is characterized by comprising an iron-carbon reaction tank, a first Fenton oxidation tank and a second Fenton oxidation tank which are sequentially connected through a water pipe, iron-carbon filler is arranged in the iron-carbon reaction tank and filled with scrap iron and granular active carbon filler, and the first Fenton oxidation tank is connected with the second Fenton oxidation tank through a water pipe. The device has the advantages that ferrous sulfate is replaced by introducing the iron-carbon reaction tank, and the iron-carbon reaction tank is filled with articles which can be collected usually, so that the cost is lower than that of the ferrous sulfate; and the iron-carbon reaction tank can play roles in the following two aspects: 1, iron-carbon oxidation reaction is carried out on iron carbon and some substances in the wastewater to decompose part of organic matters, and 2, ferrous ions are generated after the reaction of sulfuric acid and iron and are provided for subsequent Fenton reaction, so that the wastewater purification cost of an enterprise is reduced on the whole, and the economic benefit of the enterprise is improved. And the purification discharge standard of the wastewater is improved.

The application discloses a kind of purification equipment of ultra-high purity quartz sand and its purification method, including immersion washing tank, the rotating drum is equipped in the immersion washing tank, the rotating drum is opened with door, motor is fixed on the immersion washing tank side plate on rotating drum side, the output shaft of motor is fixedly connected with the side of rotating drum and the side of immersion washing tank, the sealing upper plate is slidably connected on the immersion washing tank;By immersion washing tank, it can realize soaking cleaning and spraying effect by rotating drum, and double purification is carried out on quartz sand;By immersion washing tank, mixing box and sedimentation tank, acid liquid circulation is realized, resource is saved, and purification cost is reduced;By heating pipe on sealing upper plate and the PH tester that is equipped in the inside of immersion washing tank, mixing box and sedimentation tank, the temperature and PH value of acid can be effectively controlled, so that the concentration and PH value of acid always meet the requirement of quartz sand purification, temperature and concentration are strictly controlled, and high-purity purification of quartz sand is realized.

The invention discloses an in-situ leaching liquid injection system and method for high-altitude ionic rare earth ore, and belongs to the field of rare earth hydrometallurgy. The system is composed of a liquid injection well pattern, a layered liquid injection device, a first liquid supply system, a second liquid supply system and a collection system. Exploring the thickness of an ore body weathering crust and vertical distribution of rare earth / impurities; the liquid injection hole is vertically divided into an upper layer liquid injection area (1 / 3-1 / 2 of the upper part) and a lower layer liquid injection area (1 / 2-2 / 3 of the lower part); a 1.0-1.4 wt% low-concentration diethylenetriamine-ammonium acetate composite leaching agent is injected into the upper layer, and co-leaching of impurities such as aluminum and iron is reduced; 1.5-2.5 wt% of high-concentration diethylenetriamine-ammonium acetate composite leaching agent is injected into the lower layer to ensure efficient leaching of rare earth; and rare earth-rich leaching liquid is collected. Compared with a traditional full-layer high-concentration process, the method has the advantages that the use amount of a leaching agent is reduced by 20-40% while the rare earth leaching rate is kept to be larger than or equal to 90%, the aluminum ion concentration of the leachate is reduced by 30-60%, the agent cost and the follow-up impurity removal load are remarkably reduced, and the method is particularly suitable for the high-altitude, low-temperature, low-air-pressure and thick-weathered crust environment.

The invention provides a preparation method and application of amino acid derived carbon dot nano-enzyme, and belongs to the technical field of new biomedical materials. The amino acid comprises one of histidine, proline, cysteine, tryptophan, aspartic acid, arginine, lysine, methionine and phenylalanine; the mass ratio of the amino acid to the citric acid monohydrate is (1: 1)-(1: 5). The amino acid derived carbon dots provided by the invention have free radical scavenging activity and superoxide dismutase-like properties, and show low toxicity and good biological safety at the levels of cells, nematodes and animals. Meanwhile, the amino acid derived carbon dot nano-enzyme also shows excellent anti-inflammatory activity, can down-regulate the inflammatory level of cells, reduce the damage of oxidative stress to nematodes, improve the intestinal flora structure, increase the richness and diversity of intestinal flora, relieve and treat DSS-induced mouse ulcerative colitis, and has a good application prospect. The compound can be further applied to preparation of drugs for treating inflammatory bowel diseases.

The present invention relates to a skid block device and method for inert gas purification, comprising a base, a heat exchanger, a cooler, an adsorption unit and a collection unit installed on the base; the heat exchanger has at least two groups of air inlets and air outlets, one of which is connected to the inlet end of the inert gas, one of which and the other are respectively connected to the inlet and outlet of the adsorption unit, and the other is connected to the cooler. The skid block device and method for inert gas purification provided by the present invention adopts a skid block design, has simple equipment, solves the problems of equipment redundancy and large floor space, and has the advantage of low purification cost.

This invention discloses the application of a Ni-based catalyst in the anti-CO poisoning hydrogenation reaction of a liquid organic hydrogen support, aiming to solve the technical problem of CO poisoning in existing LOHC hydrogenation catalysts. It uses CO-containing crude hydrogen as the hydrogen source to achieve the hydrogenation reaction. The Ni-based catalyst has an M / Ni reverse-phase structure, using hydrogen-dissociated metallic Ni as the support, and loading nano-metal oxide M as the supported phase to construct reverse-phase interfacial active sites. The metal M is a combination of Zr and Al, with the molar fraction of the supported phase metal M ranging from 10% to 25% based on 100% of all metals in the catalyst. The catalyst preparation method of this invention is simple and easy to implement, possessing a non-traditional supported reverse-phase structure. When applied to the anti-CO poisoning hydrogenation reaction, it exhibits superior activity compared to traditional supported catalysts. Furthermore, the reaction system is clean, safe, and reproducible, allowing for effective recycling and making it suitable for industrial production, showing promising application prospects.

The application discloses a zinc powder automatic control method, device and system for antimony salt purification process, which comprises the following steps: constructing a copper and cobalt removal fitting model, calculating the optimal potential setting value by using a particle swarm algorithm and setting the potential setting value; updating the potential setting value by using a fuzzy algorithm and a case reasoning method; constructing a three-dimensional fuzzy rule according to the difference between the current potential measurement value and the potential setting value, generating a zinc powder adjustment amount, adding the current zinc powder addition amount to obtain a new zinc powder amount setting value, writing the new zinc powder amount setting value into a DCS system on line, and controlling the automatic feeding device to add zinc powder. The above steps are repeated to achieve stable potential control and realize the minimum zinc powder consumption. The zinc powder automatic control method for the antimony salt purification process, which automatically adjusts the zinc powder addition amount according to the potential fluctuation, realizes accurate control of the zinc powder addition amount, effectively reduces the zinc powder consumption in zinc hydrometallurgy, and reduces the production cost in the antimony salt purification process.

A device capable of reducing the antimony content in industrial metal arsenic and a production method thereof, the device comprising a heating furnace (1), a vacuum tank (2) with a lower middle portion located in the heating furnace and an upper portion extending out and exposed to the air, a volatilization inner sleeve (12) composed of a charging crucible (14) at the lower portion, a metal arsenic sublimation distributor (3) at the middle portion, and a tail gas condensation distribution baffle (6) at the top, a material collection plate (9) arranged between the vacuum tank and the volatilization inner sleeve and located at the upper portion of the vacuum tank, a cover plate (7) arranged at the top of the vacuum tank, and a vacuum pumping tube (8) arranged on the cover plate; a condensation diversion sieve plate (4) is arranged between the charging crucible (14) and the metal arsenic sublimation distributor. The device of the present invention has a reasonable structure, proper temperature gradient distribution, a short process flow, high production efficiency, high product quality after industrial metal arsenic treatment, good impurity separation effect, and low purification cost.

The present invention discloses a device for removing microplastics in water, comprising a main component, comprising a filter housing, each surface of the filter housing is a mesh grille, the left end of the filter housing is connected to an openable door body; a filter component, comprising a filter grille net fixedly connected to the inner side of the filter housing and arranged close to the door body, the filter grille net is movably connected to three filter plates arranged in sequence from left to right in the filter housing on the side away from the door body; the use of the present invention can reduce microplastics in river water and purify the river, and solves the problem that the prior art is still at the research stage of detecting the microplastic content in the water after collecting a small amount of river water for sampling, and cannot further purify the river.

The invention relates to a method for recovering lead, zinc and cadmium from ammonia-process zinc smelting purification slag, which comprises the following steps: carrying out first ball milling on the ammonia-process zinc smelting purification slag, returning the obtained refined purification slag to carry out purification treatment on ammonia-process zinc smelting leachate, and separating to obtain secondary purification slag; and the secondary purification residues are sequentially subjected to second ball milling and leaching treatment, obtained leaching liquid is subjected to cadmium replacement treatment and separation to obtain replaced liquid, the replaced liquid is returned to the leaching treatment until the zinc content is larger than 100 g / L, then evaporation concentration and cooling crystallization are sequentially conducted, and separation is conducted to obtain a crystal product containing zinc sulfate heptahydrate. According to the method provided by the invention, the purification slag containing lead, cadmium and zinc can be comprehensively recycled, metal zinc in the purification slag is returned to an ammonia-process zinc smelting system for secondary purification, the use amount of an auxiliary material zinc powder can be reduced, part of zinc in the obtained purification liquid is recycled to a zinc main system for producing zinc ingots, and the production cost is reduced. And meanwhile, sponge lead, sponge cadmium, zinc sulfate heptahydrate and other products are obtained, and remarkable economic benefits are achieved.

This application discloses a multi-module combined aquaculture water purification method and system, relating to the field of water purification technology. The method includes: acquiring the rated aquaculture load and module distribution information of multiple aquaculture modules; determining the initial connection topology between the multiple water purification modules through a priori empirical model; collecting real-time monitoring data from the multiple aquaculture modules and calculating the real-time water quality load; optimizing water purification parameters based on simulation analysis; and controlling the aquaculture water purification in the target scenario based on the optimized water purification parameter results. This solves the technical problem of traditional aquaculture water purification methods being unable to dynamically adjust according to the actual load and distribution characteristics of different aquaculture modules, leading to low purification efficiency.

The invention discloses an aminoglycoside 2 '-phosphotransferase mutant and application thereof, and relates to the technical field of biotransformation, a preparation method of the aminoglycoside 2'-phosphotransferase mutant comprises the following steps: S1, cloning and expressing aminoglycoside 2 '-phosphotransferase; s2, purification of aminoglycoside 2 '-phosphotransferase; s3, determining the enzyme activity of aminoglycoside 2 '-phosphotransferase and the specificity of a substrate; s4, constructing a single point mutant; and S5, construction, expression and screening of an ApIf mutation library. The aminoglycoside 2 '-phosphotransferase mutant capable of specifically degrading GTP without degrading UTP is obtained through cloning, expression, purification and screening, and the problems that GTP is difficult to separate and purify in the UTP production process, the GTP impurity content is high in the UTP production process, and the yield and purity of UTP are low can be solved.

The invention discloses a preparation method of a carbon dioxide hydrogenation reaction catalyst. The preparation method of the oxygen carrier comprises the following steps: placing one or more of particles manually loaded with a copper element or natural ore particles and blank alumina pellets in a fluidized bed reactor according to a ratio, introducing methane gas, and jointly fluidizing at 600 DEG C for 2 hours; and after the reaction is finished and cooling, introducing nitrogen, and adjusting the gas speed until particles are layered to obtain the product catalyst. According to the method, inert particles which are originally wasted in the chemical looping combustion industry can become core components for preparing the catalyst, a special carrier does not need to be additionally purchased, the raw material cost of the catalyst is remarkably reduced, active elements on the surfaces of the prepared particles are uniformly loaded, the wear rate is smaller than 0.2% / h, and the method is a catalyst preparation method oriented to industrial application.

The present invention discloses a method and device for recovering special gases adsorbed by molecular sieves, which mainly adopt hydrogen or helium as carrier gas, blow into the molecular sieve adsorption column at room temperature to impact the special gases in the molecular sieve, thereby directly recovering the special gases in the molecular sieve at room temperature, with low recovery cost, easy operation, environmental protection and safety, recovery rate of up to 90% or more, and considerable economic benefits. Since the recovery method of the present invention does not require heating, other impurities such as moisture adsorbed in the molecular sieve are not easy to desorb, so that the impurity content of the recovered special gas is low and the utilization value is high. In addition, the carrier gas used in the present invention will not introduce impurities, and the subsequent direct low-temperature condensation of the special gas can separate the special gas from the carrier gas, and the separated carrier can be directly recycled. It can be seen that the present invention makes up for the technical defect that the current industry cannot directly recover the special gas adsorbed in the molecular sieve, and also greatly reduces the purification cost of the special gas.

This invention relates to the field of organic compound preparation technology, specifically a method for preparing 11-triglycerides of carbonate via low-temperature acylation. This method uses glycerol and undecanoyl chloride as raw materials, employing a synergistic system of a "composite acid-binding agent + Lewis acid catalyst," and combines in-situ dehydration technology and a stepwise heating strategy in a dual-solvent environment to achieve efficient synthesis. The specific steps include: under nitrogen protection, dissolving glycerol in a dichloromethane-toluene dual solvent, adding a composite acid-binding agent of pyridine and N,N-diisopropylethylamine, anhydrous zinc chloride catalyst, and 4A molecular sieve for in-situ dehydration; dropwise adding undecanoyl chloride at a low temperature of 5-10°C, followed by sequential heating to 30°C for 2-3 hours and then reacting at 50°C for 12-16 hours; and post-processing using a combined process of filtration-multi-stage extraction-membrane separation-column chromatography. This invention provides mild reaction conditions, effectively suppresses side reactions, produces high-purity products, increases molar yield, and reduces solvent toxicity and purification costs.

The invention relates to an application method of electrolytic aluminum fluorine-containing waste residues. The method comprises the following steps: preparing a sodium hydroxide solution, adding fluorine-containing waste residue powder into the solution, activating for 1-2 hours, filtering to obtain activated residues, and measuring the moisture and fluorine content of the activated residues; dissolving the lithium iron phosphate battery powder with sulfuric acid, and filtering to obtain carbon powder and leachate; adding sodium carbonate into the leachate to control the pH value of the leachate to be 1.5-2, adding activated slag, and stirring to remove aluminum; filtering the aluminum-removed slurry to obtain purified liquid and aluminum-removed slag, adding an oxidizing agent into the purified liquid to prepare iron phosphate, and taking iron phosphate synthesis mother liquor as an acidic lithium sulfate solution 1; leaching the aluminum-removed slag by adopting sulfuric acid, removing fluorine by adopting calcium carbonate, filtering to obtain a lithium sulfate solution 2 and tailings, and feeding the tailings to a cement plant as a cement raw material; mixing the lithium sulfate solution 1 and the lithium sulfate solution 2, removing impurities again, and enabling the purified solution to enter an MVR concentration system to obtain a lithium sulfate solution with the lithium concentration of 15-20g / L; and adding sodium carbonate into the lithium sulfate solution, stirring and precipitating lithium, and filtering to obtain a lithium carbonate product.

The present invention relates to the technical field of brine purification systems, and in particular to a flue gas method low-nitrate high-magnesium-calcium brine purification system, comprising a primary brine preparation device and a refined brine preparation device which are interconnected; the primary brine preparation device comprises a first heat exchanger, a first preparation unit for preparing a primary degradation aid and a first reaction unit for preparing primary brine, the input end of the upper shell side of the first heat exchanger is connected to an external cooling medium, the output end of the upper shell side of the first heat exchanger is connected to the first preparation unit, and the input end of the upper tube side of the first heat exchanger is connected to raw brine. When in use, the primary brine is prepared by the primary brine preparation device, and then the primary brine is prepared by the refined brine preparation device, which solves the problems of high salt mud powder particle content and slow sedimentation; too long brine purification cycle; low clarity, easy "back mixing" during brine conversion, affecting the quality of liquid salt, etc.; it also solves the problem of frequent flushing of tubular filters.

The invention relates to a system and a method for preparing methanol from biomass. The system for preparing methanol from biomass comprises at least one pyrolysis carbonization unit and a gasification synthesis unit; the pyrolysis carbonization unit comprises a pyrolysis reactor; the gasification synthesis unit comprises a second gasification reactor, a reforming reactor and a synthesis reactor; the preparation method of methanol comprises the following steps: S1, carrying out pyrolysis carbonization on primarily crushed biomass in a pyrolysis reactor to obtain biochar and pyrolysis gas; s2, the charcoal, oxygen and steam are subjected to a pressurized gasification reaction in the second gasification reactor, and second gasified gas is obtained; s3, the second gasified gas is subjected to a reforming reaction in the reforming reactor, and synthesis gas is obtained; and S4, carrying out a synthesis reaction on the synthesis gas in the synthesis reactor. The methanol production process can be amplified on a large scale, and the production cost is reduced.

The invention belongs to the field of biotechnology and enzymology, and particularly relates to a thermostable variant of MDH and application thereof. The amino acid sequence of the mutant is shown as any one of SEQ ID NO: 1 to SEQ ID NO: 23. The catalyst has a normal MDH catalysis function and shows high temperature stability. The thermal stability variant of MDH provided by the invention can be used for replacing traditional MDH to be applied to clinical diagnostic reagents, cold-chain transportation and low-temperature storage are avoided, the cost is reduced, and the shelf life is prolonged.

The invention discloses a method for purifying lithium diallyl malonate borate, which comprises the following steps of: 1, adding a first mixed solvent which comprises a high-polarity organic solvent and a low-polarity organic solvent in a mass ratio of (1: 1)-(1: 5) into lithium diallyl malonate borate crude salt, heating, stirring, dissolving and filtering to remove organic impurity filtrate; 2, the filter cake is subjected to vacuum drying and then ground into fine powder, a second mixed solvent is added, cooling and stirring are conducted, the second mixed solvent comprises a high-polarity organic solvent and a low-polarity organic solvent, and the mass ratio of the high-polarity organic solvent to the low-polarity organic solvent is 9: 1-3: 1; filtering while cold to remove insoluble impurity salt; and 3, concentrating the filtrate into particles, grinding the particles into fine powder, and transferring the fine powder to a vacuum drying oven for drying to obtain a lithium bis (malonate) borate finished product. The method has the advantages that the product purity is high, the metal ion content is low, the operation steps are simple, the purification cost is low, and the use requirements of the electrolyte additive are met.