1853 results about "Acylation" patented technology

The present invention belongs to the technical field of biomedical materials. Disclosed are a methacrylate, a succinate-diesterified starch, a preparation method therefor and the use thereof in an in-situ filler for corneal defects. The methacrylate, succinate-diesterified starch is prepared by means of performing graft carboxylation on starch and then grafting a double bond. The in-situ filler for corneal defects consists of the methacrylate succinate-diesterified starch (StacMA), gelatin methacryloyl (GelMA), a photoinitiator and a solvent. The corneal filler has a good biocompatibility, an excellent photoresponsive adhesion property and excellent mechanical properties, and can be used for promoting the regeneration of the natural structure of a damaged cornea and the reconstruction of visual function.

The invention relates to the technical field of organic synthesis, and discloses a preparation method of bromo-pyrrole nitrile. The method comprises the following steps: (1) mixing p-chlorophenylglycine, acetonitrile, trifluoroacetic acid and a catalyst, then dropwise adding acyl chloride and carrying out acylation reaction, and after the reaction is completed, purging with an inert gas; (2) under the inert gas, mixing the mixture obtained in the step (1) with inorganic salt, then dropwise adding 2-chloroacrylonitrile, carrying out cyclization reaction, and separating a liquid phase from the obtained mixture; and (3) under the inert gas, mixing the liquid phase obtained in the step (2), hydrobromic acid and hydrogen peroxide, carrying out a bromination reaction, then extracting with acetonitrile to obtain an acetonitrile layer, then removing the acetonitrile, and then carrying out cooling crystallization to obtain the bromo-pyrrolenitrile. According to the technical scheme, the reaction yield is high, different solvents do not need to be frequently replaced, and the solvents can be recycled.

A method for separating amoxicillin and phenylacetic acid from reaction solution in one-step enzymatic synthesis of amoxicillin is provided. The method employs immobilized penicillin acylase mutant to catalyze the one-step synthesis of amoxicillin from penicillin potassium, and develops a separation process for the resulting reaction mixture. The technical scheme mainly comprises: Firstly separating the immobilized penicillin acylase mutant from the reaction solution through filtration; subsequently isolating amoxicillin via crystallization; followed by separating and recovering phenylacetic acid through toluene extraction and back extraction. This separation method enables rapid and efficient isolation of amoxicillin with high production yield, achieving an average crystallization rate of 93.22%. Concurrently, it demonstrates effective separation and recovery of phenylacetic acid while allowing recyclable use of the toluene extractant.

The invention discloses a hydrogel compound, a preparation method and application, and belongs to the technical field of hydrogel. The hydrogel compound provided by the invention is prepared from the following raw materials: a hydrogel matrix, sodium glycerophosphate pentahydrate and a functional drug, the hydrogel matrix is prepared from chitosan, hydroxybutyl chitosan and methacrylated gelatin; the functional medicines comprise cannabidiol, tobramycin and acetylsalicylic acid; the hydrogel compound provided by the invention has excellent mechanical properties and water vapor permeability, also has excellent slow release property and wound healing ability, and can be widely applied to preparation of medicines for treating wounds. In addition, the preparation method of the hydrogel compound provided by the invention is simple to operate and beneficial to practical application.

The invention discloses a self-healing injectable photo-crosslinking dual-network hydrogel and a preparation method thereof.The preparation method comprises the steps that silk fibroin is subjected to methacrylation modification through a glycidyl methacrylate solution, then dextran of the silk fibroin is subjected to an oxidation reaction through a sodium periodate solution, oxidized dextran is generated, and the self-healing injectable photo-crosslinking dual-network hydrogel is obtained; the amino group in the modified silk fibroin and the aldehyde group of the oxidized dextran are subjected to a Schiff base cross-linking reaction to form the double-network hydrogel. The hydrogel with the double-network structure prepared by the invention is more compact in structure, so that the hydrogel has a series of excellent performances such as injectability, relatively good adhesion, good mechanical property, self-healing property and slow release property after drug loading while having safety.

The invention discloses a bacteria surface modification method based on atom transfer radical polymerization reaction mediation, obtained bacteria and application. The modification method comprises the following steps: carrying out acryloylation on the surfaces of the bacteria by using N-hydroxysuccinimide acrylate, and introducing double bonds on the surfaces of the bacteria to obtain acryloylated bacteria; the method comprises the following steps: by taking acrylamide, N-(3-aminopropyl) methacrylamide hydrochloride and N, N '-methylene bisacrylamide as monomers, carrying out polymerization reaction on the surfaces of acrylated bacteria in the presence of an initiator and a catalyst; the mass ratio of the acrylamide to the N-(3-aminopropyl) methacrylamide hydrochloride to the N, N '-methylene bisacrylamide is (11.9 to 17.5) to (0.125 to 1) to (0.1 to 0.5). According to the method, bacteria can be camouflaged to reduce immunogenicity and pathogenicity of the bacteria, the safety of bacteria treatment is improved under the condition that the viability and multiplication capacity of the bacteria are not affected, the resistance of the bacteria to environmental threats is enhanced, and the bacteria are endowed with no exogenous functions.

The invention discloses a glue solution for a copper-clad plate and a preparation method of the glue solution, and relates to the technical field of copper-clad plates. According to the prepared glue solution for the copper-clad plate, the composite epoxy resin, the curing agent, the accelerant, the solvent, the flexibilizer and the inorganic filler, the composite epoxy resin comprises bisphenol A epoxy resin, epoxy phenyl polysiloxane, itaconic acid polyaryletherketone and hydroxyl-terminated fluorine-containing vinyl polysiloxane, so that the interface bonding force with the copper plate is enhanced; the dielectric constant and the dielectric loss are effectively reduced, the peel strength and the toughness of the material are also remarkably improved, and the toughening agent is modified polyether sulfone microspheres; the modified polyethersulfone microspheres are prepared by coating polyethersulfone microspheres with hexamethylenediamine tetrapropionamide diamine, the hexamethylenediamine tetrapropionamide diamine is prepared by carrying out acylation condensation reaction on hexamethylenediamine tetramethyl propionate and ethylenediamine, and the polyethersulfone microspheres are prepared by carrying out electrostatic spinning on a spinning solution of polyethersulfone and vinylpyridine, so that the peel strength of the glue solution is remarkably improved.

The invention provides a tightening, anti-wrinkle and soothing multi-effect integrated functional lipstick. The tightening, anti-wrinkle and soothing multi-effect integrated functional lipstick is prepared from the following components in percentage by mass: 10 to 30 percent of modified polydimethylsiloxane, 0.1 to 2 percent of modified silicon dioxide, 1 to 10 percent of pentaerythritol tetraisostearate, 10 to 35 percent of an emollient, 0.01 to 20 percent of a skin conditioner and 0.1 to 50 percent of an auxiliary agent, wherein the modified polydimethylsiloxane is obtained by copolymerizing hexadecyl methacrylate, vinyl-terminated polydimethylsiloxane and methylacryloylated sodium alginate, and the modified polydimethylsiloxane and the methylacryloylated sodium alginate are co-polymerized; the modified silicon dioxide is obtained by reacting hydroxylated silicon dioxide with alkyl acid. According to the invention, ester groups, alkyl chain segments and the like are introduced into the modified polydimethylsiloxane, so that the modified polydimethylsiloxane has good compatibility with other components, the dispersity and stability of active components can be effectively enhanced, and the effects of tightening, resisting wrinkles, relieving and the like of the modified polydimethylsiloxane are improved.

The invention discloses a one-pot preparation method of semeglutide, and relates to the field of polypeptide synthesis. The preparation method comprises the following specific steps: 1) taking Arg34GLP-1 (9-37) as a starting material, controlling the pH value of a reaction solution, enabling activated dipeptide Fmoc-His-Aib-OH to react with the starting material to obtain an acylation reaction solution, and directly carrying out next-step reaction without aftertreatment after the reaction is finished; 2) adjusting the pH value of the acylation reaction liquid I, adding a compound 2-tetrapeptide into the acylation reaction liquid I, reacting to obtain acylation reaction liquid II, and directly carrying out next-step reaction without post-treatment after the reaction is finished; (3) adding an alkaline reagent to remove the Fmoc protecting group, settling, centrifuging and drying to obtain a crude product of the semeglutide; 4) separating and purifying the crude product to obtain a refined semeglutide product; the first three steps are carried out in the same reactor. Compared with a conventional liquid-phase synthesis method, the preparation method disclosed by the invention is simpler and more convenient, short in construction period, low in output of waste liquid and waste water, greatly reduced in cost, higher in yield and suitable for large-scale production.

The invention belongs to the technical field of homogeneous derivatization of cellulose, and discloses a method for homogeneous synthesis of cellulose acetate from cellulose under catalysis of a eutectic solvent. The method comprises the following steps: dissolving cellulose in a deep eutectic solvent to form a homogeneous solution, adding an acylating agent, reacting, and adding an anti-solvent to obtain the cellulose acetate with the substitution degree of 0.42-1.81. According to the method for preparing the cellulose acetate, the solvent synthesis cost is low, the reaction condition is mild, an additional activation or hydrolysis process is not needed before and after the reaction, and the process is simple. The deep-eutectic solvent can be used as a reaction solvent and a reaction catalyst, and a new technology for homogeneous preparation of cellulose acetate is developed. The problems of high solvent cost, complex process operation, high chemical toxicity and high reaction condition requirement in preparation of cellulose acetate are solved.

The invention provides a preparation method of a Janus material for simultaneous screening of protein phosphorylation and palmitoylation modification, and belongs to the technical field of magnetic material synthesis. The method comprises the following steps: firstly, preparing MCeO2; the preparation method comprises the following steps: mixing MCeO2, hexadecyl trimethyl ammonium bromide and ammonia water to obtain a mixed solution, adding 1, 2-bis (triethoxysilane) ethane into the mixed solution for reaction to obtain a product, and dispersing the product into an NH4NO3 solution for reflux reaction to obtain Janus MCP; the preparation method comprises the following steps: reacting Janus MCP with a silane coupling agent, dissolving aminated Janus MCP and 4-ethylene sulfonyl benzoic acid in a buffer solution containing EDC and NHS, and reacting to obtain the Janus material. The preparation method is reliable, the material performance is stable, and the enrichment efficiency of the magnetic Janus on target peptide fragments (phosphorylated peptide fragments and palmitoylated peptide fragments) is remarkably improved.

The invention discloses a preparation method of 4-bromo-2-(4-chlorphenyl)-5-trifluoromethyl-1H-pyrrole-3-formonitrile, and belongs to the technical field of organic synthesis.The preparation method comprises the steps of acylation reaction, cyclization reaction, bromination reaction and recovery. The preparation method disclosed by the invention is high in safety, the prepared 4-bromo-2-(4-chlorphenyl)-5-trifluoromethyl-1H-pyrrole-3-formonitrile is high in purity and yield, the equipment investment is small, the bromination reaction speed is high, the acid-binding agent can be recycled, the drying time during recycling is short, and the water content in the recycled acid-binding agent is low.

The invention discloses a hydrogel wound dressing with a synergistic healing promoting function as well as a preparation method and application thereof. The preparation method comprises the following steps: 1, dissolving oxidized methacrylated hyaluronic acid and dopamine in deionized water to obtain a solution A; the preparation method comprises the following steps: dissolving N-isopropylacrylamide, a photoinitiator 2-hydroxy-2-methyl-1-(4-(2-hydroxyethoxy) phenyl)-1-acetone and a cross-linking agent N, N '-methylene bisacrylamide in deionized water to obtain a solution B; 2, mixing the solution A and the solution B to obtain a pre-gel solution; or dissolving polyphenylboronic acid ester nanoparticles or polyphenylboronic acid ester-kaempferol with deionized water to obtain a solution C, and mixing the solution C with the solution A and the solution B to obtain a pre-gel solution; and 3, injecting the pre-gel liquid into a mold, and illuminating to obtain the hydrogel wound dressing loaded with the nanoparticles. The hydrogel disclosed by the invention can be used for effectively solving various problems in a wound healing process and promoting rapid and high-quality healing of a wound.

The invention relates to dithiolane modified beta-cyclodextrin, a preparation method thereof and application of the dithiolane modified beta-cyclodextrin as a drug carrier. The method comprises the following steps: carrying out acylation reaction on beta-cyclodextrin and lipoic acid, and modifying different numbers of dithiolane on the beta-cyclodextrin by changing a feeding ratio; the beta-cyclodextrin has a plurality of hydroxyl groups, and the activity of the hydroxyl groups at the C6 position is relatively high. The hydrophobic cavity of the dithiolane-modified beta-cyclodextrin can be loaded with a hydrophobic small-molecule drug, and the hydrophobic small-molecule drug can be loaded into the dithiolane-modified beta-cyclodextrin under the condition of heating reflux to form a nano-drug. The disulfide bond on the surface of the nano-drug and sulfydryl on protein or lipid on the outer side of a cell membrane are subjected to dynamic covalent thiol exchange, so that the nano-drug enters cells through a thiol-mediated cellular uptake way, endosomes and lysosomes can be avoided, the drug is quickly and directly delivered to the cytoplasm, degradation of the drug in the lysosomes is reduced, and the drug delivery efficiency is improved. The utilization rate of small-molecule drugs is improved, side effects are reduced, and better curative effects are achieved.

Disclosed is an acylated insulin, a pharmaceutical formulation thereof, a pharmaceutical composition thereof with a long-acting GLP-1 compound, and a medical use of the acylated insulin, the pharmaceutical formulation and the pharmaceutical composition. Compared with insulin degludec or other insulin derivatives, the acylated insulin has an unexpected, significantly increased drug effect, a longer duration of action, a longer in vivo half-life, an excellent bioavailability, as well as better physical and chemical stabilities.

The invention discloses a sgRNA-based method for modifying and regulating gene editing, which comprises the following steps: carrying out POC acylation modification on a 2 '-OH site of sgRNA to introduce an alkynyl marker to obtain sgRNA with alkynyl; the sgRNA with alkynyl and azide are subjected to copper-catalyzed azide-alkynyl cycloaddition reaction under the action of a copper catalytic reagent to form a stable chemical cross-linked structure, so that the activity of the sgRNA is quickly closed and adjusted. According to the method, alkynyl is introduced to sgRNA and is subjected to a CuAAC reaction with multi-azide to form stable crosslinking, so that the activity of the CRISPR-Cas9 system is accurately controlled. The innovative method not only can effectively control the gene editing activity on the basis of not destroying the original structure and function of RNA, but also can significantly improve the target specificity of the CRISPR system and reduce the risk of genome damage caused by non-specific cleavage.

The invention relates to a multi-carbonyl enol compound which has a structure as shown in a general formula I. The compound has a plurality of carbonyl enol structures. The invention further provides a synthesis method of the compound, the multi-carbon-based enol compound is prepared through an acylation reaction, a Willgerodt-Kindler reaction, a Vilsmeier-Haack-Arnold reaction and a hydrolysis reaction, the intermediate reaction step does not involve a noble metal catalyst, and the synthesis route is economical and efficient. The invention also provides a diazo naphthoquinone photoinitiator prepared by esterification and condensation of the compound as a monomer and diazo naphthoquinone sulfonyl chloride, and an application of the photoinitiator in I-line photoresist configuration. The compound disclosed by the invention is highly symmetrical in structure, has a unique enol interconversion and multi-carbonyl and hydroxyl functional group coexisting structure, and has an extremely wide application prospect.

The invention discloses a preparation method of a 32P-loaded ultrathin flaky chitosan hydrogel applicator. The preparation method comprises the following steps: (1) preparing a chitosan aqueous solution; (2) dropwise adding methacrylic anhydride into the chitosan aqueous solution for reaction to obtain methylacryloylated chitosan; (3) preparing a methylacryloyl chitosan aqueous solution; (4) adding 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone into the methylacryloylated chitosan aqueous solution to obtain a chitosan hydrogel precursor solution, and (5) coating the inner wall of a mold made of a polytetrafluoroethylene material with a super-hydrophilic coating; (6) adding the Na2H32PO4 aqueous solution and the chitosan hydrogel precursor solution into a mold, stirring, and carrying out illumination curing; and (7) taking out the cured hydrogel and packaging the hydrogel between two layers of transparent adhesive tapes. According to the application, the ultrathin hydrogel applicator can be prepared, so that the blocking of the hydrogel material to rays is reduced, and the curative effect of the applicator is enhanced.

The invention discloses fish mucus-methacrylated gelatin hydrogel with lubrication and resistance reduction performance and a preparation method of the fish mucus-methacrylated gelatin hydrogel. The preparation process of the hydrogel comprises the following steps: firstly, preparing a methacrylated gelatin hydrogel (GelMA) precursor by utilizing an amidation reaction of methacrylic anhydride (MA) and gelatin (Gel), and then realizing gelation by utilizing hydrogen bond interaction of a fish mucus freeze-dried product (MC) and GelMA and photo-crosslinking of the GelMA precursor and polyethylene glycol diacrylate (PEGDA) under ultraviolet light of 365nm. The mucus-methylacryloylated gelatin hydrogel (MC-GelMA) is obtained after the mucus-methylacryloylated gelatin hydrogel is prepared. The hydrogel prepared by the preparation method disclosed by the invention has excellent swelling resistance and mechanical strength. Meanwhile, the hydrogel also has good lubrication and drag reduction performance, and can be used as a ship drag reduction material.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

A method for treating a pathogen, comprising the steps of: removing, with an acid, less than 99% of —COR1 acyl groups from nitrogen sites of a poly(2-alkyl-2-oxazoline) in a solution to form a partially protonated and partially acylated poly(2-alkyl-2-oxazoline), where R1 comprises an alkyl group; adjusting a pH of the solution to greater than 2.5; forming a film from the solution, the film comprising a total cationic charge in a range of 0.001 to 10.0 coulombs per square inch, the total cationic charge countered with counteranions; and reducing activity of the pathogen contacting the film by: greater than 9% and less than 99.99995% in less than one minute, greater than 99.999% in less than four days, and greater than 99.99995% in less than seven days.

1. A composition, which is an anthocyanin-based colorant composition comprising 50-90 mol-%, based on the total amount of anthocyanins, of pelargonidin-based anthocyanins, and wherein (i) >70 mol-% of all anthocyanins are acylated with at least one phenolic acid; and (ii) >20 mol-% of all anthocyanins are acylated with at least one hydroxycinnamic acid; and wherein the composition has a red color with a hue value H≥ in the L*C*h* color system in the range of 10-30, measured at an L*-value of (70.0±0.1) in a 0.1 mol / l trisodium citrate dihydrate buffer at pH 3 in a 1 cm-length quartz cell using Spectraflash 650 (Datacolor) in transmission mode under D65 illuminant 10 Deg. Also, the present invention relates to use of the above compositions as a food colorant.

The invention belongs to the technical field of biology, and relates to a mutant of horse-derived acylase with high pH stability, a coding gene and application of the mutant in catalytic generation of lauroyl glycine. The horse-derived acylase mutant sequence disclosed by the invention has the advantages of high enzyme activity stability in a wider pH environment, high synthesis efficiency of lauroyl glycine and very high production and application values.

The invention belongs to the technical field of compound synthesis, and particularly relates to a synthesis method of AEA-AEA. Triethylene glycol is adopted as a starting material, in the first step, p-toluenesulfonate with high leaving property is formed through TsCl, then nucleophilic substitution is performed, and a product 2 is obtained; the preparation method comprises the following steps: preparing a product 3, oxidizing by adopting TEMPO capable of being industrially produced, removing Pht through quantitative hydrazine hydrate to obtain a product 4, esterifying and extracting to obtain a product 5, acylating through the product 3 to obtain amide, washing, extracting and pulping to obtain an esterified product 6; and finally, quantitatively removing Pht, hydrolyzing and pulping to obtain a pure product. According to the method, complex operation steps of using metal hydride and alkylating column passing are avoided, process production can be realized, and the product yield and purity are relatively high.

The present invention discloses a pimobendan intermediate synthesis method, which comprises: 1, carrying out a Friedel-Crafts acylation reaction on a compound I and a compound A in an aprotic solvent under the effect of a Lewis acid to generate a compound II; 2, ether is used as a solvent, under the alkaline condition, the compound II and dimethyl malonate or diethyl malonate are subjected to a substitution reaction, the generated compound is subjected to a hydrolysis reaction in an aqueous alkali solution, and a compound III is generated; and 3, heating the compound III under the conditions of hydrazine hydrate and acid to generate a compound IV, namely the pimobendan intermediate. The synthesis method cancels a nitration reaction step which is high in risk and generates a large amount of waste acid water, and is short in route step and simple to operate.

The invention discloses a preparation method of 1alpha-hydroxyl dinorhydrin, the structural formula of the 1alpha-hydroxyl dinorhydrin is shown as a formula (1), the method comprises the following steps: by taking dinorhydrin shown as a formula (2) as a raw material, preparing the 1alpha-hydroxyl dinorhydrin through five steps of acylation, bromination, debromination, epoxidation / hydrolysis and ring opening, and the reaction formula is as follows: # imgabs0 #. All reactions are carried out under normal pressure, reagents with high toxicity are not used in the reaction process, the reaction yield is high, the total yield of the five-step reaction can reach 48.46%, and the method is suitable for industrial production.

The invention relates to the technical field of bentonite anti-seepage materials, and particularly discloses a pre-hydrated bentonite anti-seepage material and a preparation process thereof. The modified bentonite is prepared by mixing the following raw materials in parts by mass: 80-90 parts of calcium bentonite, 10-12 parts of a binder, 5-8 parts of an organic composite component and 3-5 parts of ethylene carbonate, the preparation method comprises the following steps: taking calcium bentonite, crushing, sieving, adding water for dispersing, adjusting the pH value, adding ethylene carbonate, heating and stirring, adding the organic composite component, continuously treating, filtering, taking a solid part, adding a binder, mixing, laminating, carrying out mold pressing treatment, and cooling, thereby obtaining the product. Wherein the organic composite component is obtained by amino-terminated modification of acylated polyethylene glycol. The water absorption rate of the pre-hydrated bentonite anti-seepage material prepared by the preparation process is greater than or equal to 5.2, the water retention rate is greater than or equal to 63.7%, and the elution risk of intercalator micromolecules can be greatly reduced.

The invention discloses ternary synergistic nano-enzyme hydrogel as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The preparation method comprises the following steps: dissolving zinc nitrate hexahydrate and ferric nitrate nonahydrate in methanol; dissolving 2-methylimidazole in methanol, adding into a metal salt solution, stirring, and sequentially centrifuging, washing and drying to obtain Fe / ZIF-8 nano-enzyme; the preparation method comprises the following steps: adding methacrylated gelatin and hyaluronic acid into deionized water, stirring, cooling to room temperature, and refrigerating to obtain a GelMA-HA precursor solution; and adding the Fe / ZIF-8 nano-enzyme into the GelMA-HA precursor solution, stirring, injecting into a mold, and carrying out ultraviolet light cross-linking curing to obtain the ternary synergistic nano-enzyme hydrogel. According to the hydrogel disclosed by the invention, active oxygen is efficiently removed and oxygen is produced through Fe / ZIF-8 nano-enzyme, and cartilage repair is promoted in combination with lubrication and slow release functions of gelatin hydrogel, so that a new strategy is provided for treatment of osteoarthritis.

The invention relates to the technical field of medical interventional therapy materials, and particularly discloses a preparation method of sodium hyaluronate embolism microspheres and the prepared embolism microspheres. The preparation method of the sodium hyaluronate embolization microspheres comprises the following steps: S1, adding methacrylic anhydride, N, N-dimethylformamide and sodium hydroxide into a sodium hyaluronate aqueous solution, reacting, adding sodium chloride, reacting, adding absolute ethyl alcohol, precipitating, filtering to remove filtrate, cleaning and drying; s2, preparing a mixture of methacrylated sodium hyaluronate, a monomer with negative charges, an initiator and water into a water phase; a mixture of oil soluble liquid and a dispersing agent is used as an oil phase; and under the protection of nitrogen, carrying out reverse suspension polymerization. According to the preparation method of the sodium hyaluronate embolization microspheres, the methylacryloylated sodium hyaluronate and the negatively charged monomer are copolymerized to introduce the negatively charged group, so that the microspheres can effectively load chemotherapeutic drugs, and the compression elasticity is high.

The invention discloses an ROS-responsive edible antibacterial preservative film and a preparation method thereof. The invention discloses a ROS (reactive oxygen species)-responsive antibacterial agent which is composed of sodium alginate (SA), carboxymethyl chitosan (CS), an ROS response group (TK-NH2) and antibacterial micromolecules (AB), TK-SA with ROS response characteristics is obtained through acylation reaction of TK-NH2 and SA through EDC, the antibacterial micromolecules (AB) are loaded into TK-SA through acylation reaction of EDC, and AB-TK-SA with antibacterial activity is obtained; aB-TK-SA and CS are mixed, sprayed to a clean glass plate and dried at the room temperature. The ROS-responsive edible antibacterial preservative film (AB-TK-SA / CS) provided by the invention has the characteristics of safety, low toxicity and ROS-responsive release, and can effectively inhibit the growth of staphylococcus aureus; the method is simple and convenient in preparation process, low in cost and good in reproducibility, and has a good application prospect in the field of related food preservation.