181 results about "Methacrylic anhydride" patented technology

The invention relates to a double-layer dressing for promoting wound healing and inhibiting scars and a preparation method and application thereof.The double-layer dressing comprises a hydrogel layer and a piezoelectric film layer compounded on the hydrogel layer, and the piezoelectric film layer is prepared by mixing an organic piezoelectric material, a ferroelectric material and an organic solvent and then conducting an electrostatic spinning technology; the hydrogel layer is formed by curing a methacrylic anhydride acellular extracellular matrix, methacrylic anhydride catechol chitosan and conductive nanoparticles under the action of a curing agent. The lower skin contact layer of the double-layer dressing is the hydrogel layer, so that the directional growth of fibroblasts is favorably induced; the upper dressing is a piezoelectric material composite ferroelectric material, is beneficial to piezoelectric stimulation re-induction function recovery after final skin repair and guidance of directional growth of wound cells, and has good skin wound induction repair and scar inhibition effects.

The invention provides a methacrylic anhydride modified sclera extracellular matrix repair material and a curing method thereof, and belongs to the technical field of medical repair materials. The methacrylic anhydride modified sclera extracellular matrix repair material provided by the invention is prepared from the following raw materials: a methacrylic anhydride modified sclera extracellular matrix, methacrylated gelatin, a photoinitiator and a solvent. The sclera extracellular matrix modified by methacrylic anhydride is adopted, methacrylic anhydride contains methacrylic acid, double bonds can be introduced, the sclera extracellular matrix has the photo-initiation polymerization capacity, and in-situ gelling can be carried out at sclera and cornea damage positions under the action of a photoinitiator; the sclera extracellular matrix has good biocompatibility and histocompatibility, so that sclera matrix cells and corneal epithelial cells are adhered and proliferated, and the sutureless repair is realized; the methacrylated gelatin can provide a photo-crosslinking gelatin network under the action of a photoinitiator, so that cell adhesion and proliferation can be promoted, and repair is realized.

The invention discloses polysaccharide-based double-network hydrogel as well as a preparation method and application thereof. The polysaccharide-based double-network hydrogel comprises a first gel network and a second gel network interwoven with the first gel network, the first gel network is formed by crosslinking modified chitosan and modified hyaluronic acid through a Schiff base reaction, and the second gel network is formed by crosslinking modified chitosan and modified hyaluronic acid through a photopolymerization reaction; wherein the modified chitosan is chitosan modified by glycidyl methacrylate or chitosan which is coupled with a cartilage regeneration promoting substance and is modified by glycidyl methacrylate, and the modified hyaluronic acid is formylated hyaluronic acid modified by methacrylic anhydride. The hydrogel prepared by the invention has controllable aperture, proper swelling property and long-term stable drug sustained release effect, can effectively maintain the activity of mesenchymal stem cells, has good in-vivo biocompatibility, and can significantly promote cartilage regeneration.

The present invention pertains to the technical field of materials. Disclosed are a polymer composite hydrogel and a preparation method therefor, and a tooth desensitization material. The polymer composite hydrogel consists of a GelMA hydrogel and BP nanosheets composited inside the GelMA hydrogel, wherein the GelMA hydrogel is prepared by crosslinking gelatin methacryloyl via double bonds under the action of a photoinitiator. Under the photothermal effect, the polymer composite hydrogel of the present invention can enhance the resistance of dentin to external stimuli and promote the formation of stable and effective occlusion of dentinal tubules. Moreover, the occlusion effect on the dentinal tubules exhibits acid resistance, corrosion resistance, and stability, preventing the possibility of short-term reopening of the occluded dentinal tubules. This is of great significance for the treatment of occluded dentin hypersensitivity (DH).

The invention relates to the technical field of bone tissue materials, and discloses a metal-doped biomimetic mineralized dECM cryogel and a preparation method and application thereof.The preparation method of the cryogel comprises the steps that firstly, hyaluronic acid and methacrylic anhydride are mixed in a solvent, the pH value is adjusted, a reaction is conducted, and methacrylic acid hyaluronic acid is obtained; then under the condition of a photoinitiator, mixing the methacrylic acid hyaluronic acid with an acellular extracellular matrix, and performing freezing and crosslinking treatment to obtain the cryogel, and finally, soaking the cryogel in a mineralization solution containing Ca < 2 + > and / or Mg < 2 + >, adding a buffer solution containing phosphite ions, incubating, and washing to obtain the crystal gel. The biomimetic mineralized dECM cryogel prepared by the method disclosed by the invention is good in ion release stability, can significantly improve the migration ability of HUVECs, contributes to promoting proliferation of rMSCs and improving the osteogenic differentiation potential of the rMSCs, and can be used for regeneration and repair of bone defects.

The invention provides a GelMA microneedle patch loaded with chromogenic gold nano bipyramids and a preparation method thereof.The method comprises the steps that citric acid, chloroauric acid and a CTAC solution are mixed, then sodium borohydride is added, and a seed solution is obtained through stirring and standing; mixing chloroauric acid, silver nitrate, hydrochloric acid and CTAB (cetyltrimethyl ammonium bromide), and sequentially adding ascorbic acid and the seed solution to obtain a growth solution; carrying out centrifugation, morphology regulation and chemical etching purification to obtain a chromogenic gold nano bipyramid solution; the preparation method comprises the following steps: reacting gelatin with methacrylic anhydride in PBS, dialyzing and freeze-drying to obtain GelMA; dissolving GelMA in the gold nanoparticle bipyramid solution, and adding a photoinitiator LAP to obtain a mixed precursor solution; and the GelMA microneedle patch loaded with the gold nano bipyramid is prepared through mold casting, centrifugation, ultraviolet light crosslinking and drying demolding. The microneedle extraction and gold nano bipyramid color development integrated scheme is initiated, blood sampling is not needed, the dermis interstitial fluid is extracted in a minimally invasive mode through the microneedle, detection pain and trauma are reduced, the cross infection risk of blood treatment is avoided, and the microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle

The invention discloses a plant-based deodorant and a preparation method thereof, and relates to the technical field of high polymer materials. When the plant-based deodorant is prepared, beta-cyclodextrin reacts with paratoluensulfonyl chloride and then reacts with ethylenediamine, and amino modified cyclodextrin is prepared; the plant-based extract is subjected to inclusion with amino modified cyclodextrin, and a cyclodextrin inclusion compound is prepared; the preparation method comprises the following steps: reacting hydroxyethyl cellulose with methacrylic anhydride to prepare double-bond modified cellulose; carrying out hydrothermal reaction on titanium sulfate, the carboxylated multi-walled carbon nanotube, ammonium fluoride and cerous nitrate to prepare a co-doped titanium dioxide / carbon nanotube; coating the co-doped titanium dioxide / carbon nano-tubes with oleic acid to prepare modified nano-particles; and reacting the double-bond modified cellulose with the cyclodextrin inclusion compound, and then copolymerizing with the modified nanoparticles and the comonomer to obtain the plant-based deodorant. The prepared plant-based deodorant has the advantages of good deodorization effect, slow release and photocatalytic degradation.

The invention discloses injectable hydrogel with a myocardial tissue repairing function as well as a preparation method and application of the injectable hydrogel, and relates to the technical field of medical materials. The preparation method of the injectable hydrogel comprises the following steps: carrying out a reaction on deferoxamine and DSPE-PEG-NHS, so as to obtain DSPE-PEG-DFO; the preparation method comprises the following steps: wrapping carvedilol with DSPE-PEG-DFO under the hydrophilic and hydrophobic action, so as to obtain a drug-loaded nano-micelle; the preparation method comprises the following steps: reacting a polymer containing amino or hydroxyl with methacrylic anhydride to obtain a methylacryloyl modified polymer; reacting the carboxyl-containing polymer with cysteamine hydrochloride to obtain a sulfydryl modified polymer; mixing the drug-loaded nano-micelle, the methylacryloyl modified polymer and the sulfydryl modified polymer, and reacting under ultraviolet radiation to obtain the injectable hydrogel. The hydrogel disclosed by the invention is good in biocompatibility, can release deferoxamine and carvedilol in a ladder manner, is matched with a myocardial repair process, and realizes a function of repairing damaged myocardial tissues.

The invention discloses a decellularized amniotic membrane matrix composite scaffold, a preparation method thereof and application of the decellularized amniotic membrane matrix composite scaffold in orbital bone defect repair, and relates to the technical field of biomedical engineering and biomedical materials. In order to solve the limitation of an existing orbit implant material, methacrylic anhydride (MA) is grafted to HAM, methacrylate HAMMA hydrogel is synthesized, and then the decellularized amnion matrix composite scaffold (HAMMA / HAP / BMP-2) is prepared by combining a simulated body fluid biomimetic mineralization method with 3D printing and ultraviolet curing. The composite scaffold material prepared by the invention can regulate the release of BMP-2 and maintain the effective drug concentration of a bone defect area so as to regulate the immune microenvironment of an early bone defect repair area, collect osteoblasts and accelerate the repair of bone defects.

The invention relates to an intumescent efficient hemostatic sponge and a preparation method thereof, the preparation method comprises the following steps: preparation of HAMA: utilizing hyaluronic acid to react with methacrylic anhydride, and grafting double bonds on a hyaluronic acid macromolecular skeleton; preparing HAMA hydrogel: pouring the HAMA solution dissolved with the photoinitiator into a mold, and initiating crosslinking in an ultraviolet radiation manner to obtain the HAMA hydrogel; the HAMA hydrogel is taken out of the mold and dried, and the hemostatic sponge is formed. The expansion type efficient hemostatic sponge can be prepared by adopting the preparation method. The hemostatic sponge disclosed by the invention shows remarkable advantages in the aspects of expansion ratio, hemostatic effect, biocompatibility and the like, and has a wide application prospect and an important clinical value. The preparation method disclosed by the invention is easy to operate in process flow and suitable for large-scale production.

The invention discloses a polycarbonate-based non-isocyanate polyurethane ultraviolet curing coating and a preparation method of the polycarbonate-based non-isocyanate polyurethane ultraviolet curing coating. The photocureable coating is prepared from the following components: 10 to 60 percent of polycarbonate acrylate, 5 to 20 percent of urethane acrylate, 10 to 60 percent of reactive diluent, 1 to 5 percent of photoinitiator and 0 to 60 percent of solvent. Comprising the following steps: (1) reacting polycarbonate diol with methacrylic anhydride to obtain polycarbonate acrylate; (2) carbonating the allyl glycidyl ether by using carbon dioxide to obtain corresponding five-membered cyclic carbonate, and reacting with diamine to obtain urethane acrylate; and (3) uniformly mixing the obtained product with a reactive diluent and a photoinitiator to obtain the ultraviolet curing coating. The polycarbonate-based non-isocyanate polyurethane ultraviolet curing coating obtained by the invention is a biodegradable environment-friendly coating, a non-isocyanate polyurethane synthesis route is adopted, the use of isocyanate is avoided, and the coating shows excellent flexibility and pencil hardness on a PC (polycarbonate) base material.

The invention belongs to the technical field of anticorrosive paint, and discloses intelligent corrosion inhibition hydrogel with double functions of pH sensitivity and corrosion self-early warning, preparation of the intelligent corrosion inhibition hydrogel and application of the intelligent corrosion inhibition hydrogel in modified epoxy resin anticorrosive paint. The preparation method comprises the following steps: preparing a water phase from quaternary ammonium salt chitosan, methacrylic anhydride, a photoinitiator and a corrosion inhibitor, taking a Span emulsifier and paraffin as an oil phase, dropwise adding the water phase into the oil phase, rapidly stirring to form a water-in-oil emulsion system, and rapidly preparing the pH-sensitive hydrogel microspheres coated with the corrosion inhibitor through photo-crosslinking. And finally, forming the corrosion self-early-warning shell by using the tannic acid modified hydrogel microspheres by adopting an electrostatic adsorption layer-by-layer assembly method. The intelligent corrosion inhibitor hydrogel and the modified anticorrosive paint prepared by the invention have double functions of pH sensitivity and corrosion self-early warning. And meanwhile, the material is green and environment-friendly, the preparation process is simple, the cost is low, the corrosion resistance is effectively improved, the corrosion resistance is enriched, and long-acting corrosion prevention is realized.

The invention provides an acrylic polyurethane coating and a preparation method thereof, and belongs to the technical field of coating compositions.The preparation method comprises the following steps that hexylpolyurethane based on non-isocyanate sorbitol, methacrylic anhydride, p-toluenesulfonic acid and hydroquinone monomethyl ether are mixed and heated for a reaction, ethyl acetate is added for mixing, washing, drying, filtering and vacuum concentration are performed, and the acrylic polyurethane coating is obtained. The bio-based polyurethane acrylate is obtained; the preparation method comprises the following steps: mixing ZG nanoparticles, methylbenzene, 3-(methacryloyloxy) propyltrimethoxysilane and hydroquinone monomethyl ether, heating, stirring, centrifuging and washing to obtain functionalized ZG; and fully mixing the bio-based polyurethane acrylate, the photoinitiator, the acrylate-based single-terminated reactive polysiloxane, the reactive diluent and the functionalized ZG to obtain the acrylic polyurethane coating. The coating can improve the curing speed, and has hydrophobic and oleophobic properties and corrosion resistance at the same time.

The invention belongs to the technical field of biomedical materials, and particularly relates to astragaloside-loaded conductive hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing a methylacrylic anhydride gelatin aqueous solution with an organic solvent solution of astragaloside, and carrying out ultraviolet light cross-linking curing to form methylacrylic anhydride gelatin / astragaloside hydrogel; soaking the obtained hydrogel in hydrochloric acid containing a free radical initiator; then soaking and polymerizing in an aniline ethanol solution; and soaking and purifying to obtain the methylacrylic anhydride gelatin-polyaniline conductive hydrogel loaded with astragaloside. In-vitro experiments prove that the compound has a certain anti-inflammatory effect, can induce neural stem cells to be directionally differentiated into neurons and oligodendrocytes, and is beneficial to promoting nerve regeneration and myelin sheath formation; finally, in-vivo experiments prove that the composition can improve the athletic ability and tissue function recovery of SCI rats, and has a good application prospect.

The invention provides 3D printing bio-ink as well as a preparation method and application thereof. The 3D printing bio-ink is prepared from the following components: methacrylic anhydride gelatin, oxidized hyaluronic acid, carboxymethyl chitosan and 2-methacryloyloxyethyl phosphorylcholine. By designing the specific composition of the 3D printing bio-ink, the 3D printing bio-ink which is good in printing performance, mechanical strength and electrical conductivity and high in cell survival rate after printing is prepared, and then a 3D printing stent with high cell-stent interaction is prepared and can be used for preparing a skin injury repair material.

The application discloses a kind of high adhesion enzymatic hydrogel for promoting vascular intimal repair and a preparation method thereof, and belongs to the technical field of hydrogel preparation.The preparation method is as follows: S1, using methacrylic anhydride to modify the double bond of hyaluronic acid, then crosslinking with dopamine to obtain dopamine-modified double-bonded hyaluronic acid;S2, after removing oxygen from the hyaluronic acid solution, add a crosslinking agent and tyramine hydrochloride, and react in an acidic environment to obtain tyramine-modified hyaluronic acid;S3, mix the dopamine-modified double-bonded hyaluronic acid, tyramine-modified hyaluronic acid and deionized water to obtain a hydrogel precursor solution;S4, then use enzymatic reaction to obtain the hydrogel.The method designed by the application is simple, does not require additional light curing equipment, and the constructed hydrogel is similar to the extracellular matrix.The hydrogel can be coated into different shapes to adapt to different environments.The hydrogel is formed by enzymatic reaction, which is rapid, well adhered to the tissue, biocompatible, and suitable for application in vascular repair.

The present invention discloses a method for preparing a photocrosslinked hyaluronic acid / ε-polylysine antibacterial adhesive hydrogel, the method comprising first modifying hyaluronic acid with methacrylic anhydride, introducing dopamine to modify the methacrylic anhydride-modified hyaluronic acid and mixing it evenly with ε-polylysine, then adding a photoinitiator solution and mixing evenly, and irradiating it with ultraviolet light for more than ten seconds to obtain the result. The hydrogel prepared by dopamine-modified hyaluronic acid has significantly improved adhesion to tissues compared to unmodified hyaluronic acid hydrogels, and ε-polylysine can further improve the adhesion of the hydrogel by virtue of its synergistic effect with dopamine.

The invention relates to the technical field of biological materials, and discloses electrical stimulation LDH hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a zinc salt, a vanadium salt and urea in water in a nitrogen atmosphere to obtain a precursor solution; the precursor solution is subjected to a hydrothermal reaction, and ZnV-LDH is obtained; under stirring, gradually dropwise adding methacrylic anhydride into the gelatin solution, and heating for reaction; adding preheated water into the reaction liquid to stop the reaction; dialyzing and purifying the reaction liquid, pre-freezing, and freeze-drying to obtain hydrogel; and adding ZnV-LDH into the hydrogel, and carrying out ultrasonic mixing, so as to obtain the electrical stimulation LDH hydrogel. Through the synergistic effect of the ZnV-LDH and the hydrogel, the electrical stimulation LDH hydrogel disclosed by the invention has electrical activity and can generate potential change in response to external stimulation; under ultrasonic stimulation, concentration-dependent electrical response can be generated, the response has periodicity and stability, and osteogenic differentiation and cell mechanical properties can be promoted to the maximum extent.

The invention relates to the technical field of synthesis of photoresist monomer compounds, and particularly discloses a synthesis method of 1, 3-adamantanediol monomethacrylate. The synthesis method of the 1, 3-adamantane diol monomethacrylate comprises the following steps: by taking 1, 3-dihydroxy adamantane and methacrylic anhydride / methacryloyl chloride as reaction raw materials and a two-phase solvent as a reaction solvent, under the action of a catalyst and a polymerization inhibitor, carrying out esterification reaction and post-treatment to obtain the 1, 3-adamantane diol monomethacrylate. The component A is 1, 3-adamantanediol monomethacrylate; the catalyst is ethyl magnesium chloride; the temperature of the esterification reaction is 10-20 DEG C, and the time is 6-8 hours. The synthesis method of 1, 3-adamantanediol monomethacrylate provided by the invention has the advantages of mild reaction conditions, simple operation, cheap and easily available raw materials and the like, and the yield of the obtained 1, 3-adamantanediol monomethacrylate is greater than or equal to 80.0%, and the purity is greater than or equal to 99.00%.

The present invention discloses a method for preparing a starch-based 3D printing ink suitable for high printability, and belongs to the technical field of green printing materials. The present invention selects natural starch as raw material, and modifies the starch by methacrylic anhydride. The modified methacrylated starch can undergo self-crosslinking under ultraviolet light to form a network structure, and then compounded with natural starch and high-amylose starch to prepare a starch-based 3D printing ink with high printability. The ink has the characteristics of controllable photosensitivity, easy extrusion, and high formability, can be printed with high precision, is biodegradable, and uses non-toxic and pollution-free crosslinking agents and adhesives in the preparation process. It is a green material and can be safely used in biological tissue engineering. The present invention uses 3D printing technology, and can print the shape and size of printed products according to needs, which is convenient for personalized production. The printed products have high mechanical strength, high porosity and high biological stability, and have high application prospects.

The invention discloses a preparation method of silver-loaded nano-cluster-adriamycin composite hydrogel. The method comprises the following steps: preparing a silver nano-cluster from an AgNO3 solution and a gallic acid solution; preparing a prepolymer from chitosan, hyaluronic acid, an acetic acid solution and methacrylic anhydride; mixing silver nanoclusters, a doxorubicin hydrochloride solution and the prepolymer, and adding a photoinitiator to obtain preliminarily formed hydrogel; and immersing the primarily formed hydrogel into a sodium citrate solution to obtain the silver-loaded nano-cluster-adriamycin composite hydrogel. The composite hydrogel prepared by the invention has a pH-responsive drug release characteristic, the drug release is accelerated in an acidic environment, and the drug release is slow in a neutral environment; the antibacterial agent has an inhibiting effect on bacteria such as escherichia coli and staphylococcus aureus, and shows good antibacterial performance; the toxicity to cancer cells is obviously higher than that of normal cells, and certain tumor targeting is achieved; and the polymer has good mechanical strength and good swelling performance in different pH environments, and has a wide application prospect.

The present invention relates to the technical field of biomedical materials, and specifically provides a bone and joint biological super-lubricating copolymer and a preparation method thereof. Dopamine is modified with methacrylic anhydride to prepare dopamine methacrylamide, and then a positively charged poly (2-ethyl-2-oxazoline) with good hydrophilicity and hydration lubrication performance and dopamine methacrylamide with adhesion performance are subjected to free radical polymerization to form a super-lubricating copolymer. The positively charged super-lubricating copolymer has good cell affinity, can promote the attachment, growth and differentiation of chondrocytes, and is beneficial to tissue repair and regeneration. The positively charged super-lubricating copolymer has high hydrophilicity, excellent hydration ability and lubricating properties, can adhere to the joint surface, absorb surrounding water molecules to form a hydration layer, effectively reduce surface friction and wear, and the unique adhesion properties of the copolymer and the phenol structure can effectively scavenge free radicals to achieve an anti-inflammatory effect.

This invention discloses a method for preparing UV-curable modified epoxy vegetable oil acrylate. The method involves mixing acid anhydride and polyol at a molar ratio of 1:0.8-1.2, followed by rotary evaporation to remove the organic solvent, yielding a hydroxycarboxylic acid. The hydroxycarboxylic acid is then mixed with methacrylic anhydride at a molar ratio of 1:2-5, and the methacrylic acid is removed by rotary evaporation, yielding a carboxyl-containing polyvinyl prepolymer. This carboxyl-containing polyvinyl prepolymer is then reacted with epoxidized vegetable oil in the presence of a catalyst and a polymerization inhibitor until the acid value reaches 2-20 mg KOH / g, followed by cooling and discharge. This invention also discloses the UV-curable modified epoxy vegetable oil acrylate obtained by the above preparation method and a coating. The UV-curable modified epoxy vegetable oil acrylate prepared by this invention has high vinyl functionality, high crosslinking density in the cured coating film, and good alcohol resistance, making it suitable for use in wood, furniture, and plastic coatings.

The present invention relates to the technical field of joint repair hydrogels, and in particular to a three-dimensional biohydrogel and its preparation method and application. The three-dimensional biohydrogel, by weight, comprises: 1-5 parts of methacrylic anhydride hyaluronic acid HAMA, 0.05-0.15 parts of initiator, 3-8 parts of hydrogenated soybean phosphatidylcholine liposomes and 7-13 parts of stem cell solution; the preparation method of the stem cell solution is: gelatin and water are mixed to obtain a gelatin solution; stirring at 40-60 ° C to prepare a dispersed phase; adding an emulsifier to the oil to prepare a continuous phase; the dispersed phase and the continuous phase are prepared by microfluidic technology to obtain uniform droplets, and the droplets are freeze-dried to obtain gelatin microspheres; stem cells are added to the gelatin microspheres and incubated at 25-37 ° C for 2-4 hours to obtain a stem cell solution. The present invention has long-lasting efficacy and significant safety, and can fundamentally repair articular cartilage.

The present application discloses a method for preparing biodiesel using lipase catalysis, which relates to the field of biodiesel. The biodiesel enzymatic reaction process comprises: raw oil pretreatment, enzymatic transesterification reaction, acid reduction treatment, and fatty acid salt modification. The acid reduction treatment step comprises: passing the obtained crude biodiesel through an oil-water separator to further remove water, passing the dehydrated crude biodiesel into a reactor containing a low concentration of potassium hydroxide methanol solution for multiple acid reduction reactions, and obtaining acid-reduced biodiesel after the reaction. Methylacrylic anhydride and tertiary butylhydroquinone are subjected to an esterification reaction, and the synthesized product is subjected to a halogenation reaction with copper chloride. The resulting product is used to modify the fatty acid salts in the acid-reduced biodiesel to obtain the biodiesel of the present application. The present application has the effects of reducing the acidity of biodiesel, avoiding saponification reaction, improving anti-emulsification ability, and lowering the freezing point.

The invention belongs to the technical field of biomedical materials, and particularly relates to oxygen-enriched hydrogel for culturing vascular organs and a preparation method thereof.The preparation method comprises the steps that hyaluronic acid carboxyl and cysteine amino are covalently bound to obtain hyaluronic acid-cysteine conjugate HA-C, methacrylic anhydride and hydroxyl of an acellular matrix are subjected to an esterification reaction, and the hydrogel is obtained; the preparation method comprises the following steps: grafting methylacryloyl onto ECM (extracellular matrix) to obtain methylacryloyl modified acellular matrix ECM-MA; hA-C and ECM-MA are mixed and dissolved in a water phase, and a photoinitiator and PVP-coated calcium peroxide are added for cross-linking to form the oxygen-enriched hydrogel. The hydrogel is constructed by utilizing the sulfhydrylated hyaluronic acid and the double-bond functionalized acellular matrix by utilizing a click chemical reaction, and meanwhile, the calcium peroxide is added, so that the material has the function of releasing oxygen; the obtained hydrogel has biocompatibility, controllability and biological activity, and provides a high-performance material basis for biomedical application.

The invention provides a preparation method of an isobornyl methacrylate monomer, which comprises the following steps: compounding isobornyl alcohol, methacrylic anhydride, absolute ethyl alcohol, immobilized enzyme, a 3A molecular sieve and hydroquinone to obtain a reaction solution, centrifuging, filtering, separating, and carrying out rotary evaporation to obtain the isobornyl methacrylate monomer. The isobornyl methacrylate monomer is prepared by using the immobilized enzyme as the catalyst, so that the yield of the product is improved, the immobilized enzyme can be reused, and the consumption of the catalyst is reduced; meanwhile, the enzyme can be effectively prevented from being diluted or lost in the reaction process, so that the economical efficiency of the reaction is improved; in addition, the form of the immobilized catalyst is beneficial to simplifying subsequent separation and purification steps, and is relatively environment-friendly.

The application belongs to the technical field of biological medicine, and particularly relates to the use of a lung extracellular matrix hydrogel in the preparation of a lung injury repair material. The application grafts methacrylic anhydride groups on a lung tissue-derived extracellular matrix, and provides a lung extracellular matrix hydrogel which can be rapidly photo-crosslinked, has high stability, effectively promotes angiogenesis, and prevents postoperative leakage. The photo-crosslinked lung extracellular matrix hydrogel of the application is applied to a lung injury animal model, significantly promotes angiogenesis, promotes alveolar regeneration, and also significantly reduces the fibrosis level. Compared with a sealing agent alpha-cyanopropyl acrylate gel and a fibrin glue which are used in clinical application, the photo-crosslinked lung extracellular matrix hydrogel has excellent effects on repairing lung injury, provides a new strategy for repairing traumatic and iatrogenic lung injury, and has a good application prospect.

The invention discloses a preparation method of Chinese yam mucin anti-drag hydrogel. The preparation method comprises the following steps: crushing Chinese yam blocks and deionized water to obtain a Chinese yam mucus mixture; centrifuging, standing and layering, extracting supernate, and filtering to obtain a plant extracting solution; purifying by adopting a reverse osmosis method, and air-drying to obtain Chinese yam mucoprotein; the preparation method comprises the following steps: adding gelatin into a PBS solution, heating and stirring, then adding N, N-dimethylformamide and methacrylic anhydride, heating and stirring, dialyzing, and freeze-drying to obtain a gelatin derivative grafted with carbon-carbon double bonds; the preparation method comprises the following steps: adding a gelatin derivative into deionized water, heating and stirring, adding Chinese yam mucoprotein, N, N '-methylene bisacrylamide and ammonium persulfate, stirring, centrifugally defoaming, and drying to obtain the Chinese yam mucoprotein drag-reducing hydrogel. According to the preparation method of the Chinese yam mucoprotein anti-drag hydrogel, Chinese yam mucoprotein macromolecular chains are connected into a network structure by using N, N '-methylene bisacrylamide molecules, so that water-soluble Chinese yam mucoprotein is prevented from being dissolved and lost in a water body, and long-term lubrication and anti-drag are realized.

The invention relates to a one-step purified dual-modified hyaluronic acid hydrogel and a preparation method thereof, and the preparation method comprises the following steps: under a dark condition, dropwise adding a sodium periodate solution into a sodium hyaluronate solution to carry out hydroformylation reaction, and quenching to obtain a hydroformylated hyaluronic acid reaction solution; cooling the hydroformylated hyaluronic acid reaction solution to 2-6 DEG C, dropwise adding a methacrylic anhydride solution under a dark condition to perform esterification reaction, controlling the pH value of the reaction to be 8-9, and purifying and drying after the reaction is finished to obtain dual modified hyaluronic acid; and dissolving the dual-modified hyaluronic acid and a photoinitiator in a PBS buffer solution to form a precursor solution, and carrying out a photo-crosslinking reaction to obtain the dual-modified hyaluronic acid hydrogel. According to the preparation method disclosed by the invention, aldehydes are firstly subjected to esterification reaction, so that mutual interference is avoided, side reactions are reduced by controlling reaction conditions, and the obtained hydrogel has controllable gelation time, excellent mechanical properties, swelling resistance, long-term stability and good blood compatibility.