308 results about "Methacrylic anhydride" patented technology

The embodiment of the invention discloses a preparation method of exosome-loaded hydrogel, the exosome-loaded hydrogel and application of the exosome-loaded hydrogel. A specific embodiment of the method comprises the following steps: carrying out oxidation treatment on hyaluronic acid; carrying out mixed reaction on oxidized hyaluronic acid and methacrylic anhydride under a preset environmental condition to obtain a first mixed solution; performing dialysis and freeze-drying treatment on the first mixed solution to obtain target oxidized hyaluronic acid; adding the target oxidized hyaluronic acid into the exosome solution to obtain an exosome-loaded oxidized hyaluronic acid solution; the exosome-loaded oxidized hyaluronic acid solution and polylysine are subjected to a mixed reaction, the exosome-loaded hydrogel is obtained, and the mass ratio of target oxidized hyaluronic acid to polylysine in the exosome-loaded hydrogel ranges from 1 to 2. According to the embodiment, the mechanical property and controllable degradability of the exosome-loaded hydrogel can be improved, so that the applicability of the exosome-loaded hydrogel and the retention capacity and activity of the exosome are improved.

The invention discloses injectable hydrogel with redox activity and electroactivity as well as a preparation method and application of the injectable hydrogel, and belongs to the technical field of medical materials. The preparation method comprises the following steps: reacting methacrylic anhydride and polysaccharide molecules in a solvent to prepare a double-bond functional polymer; the preparation method comprises the following steps: firstly, pre-polymerizing dopamine in a solvent, and then adding MOF (Metal Organic Framework) to carry out polymerization reaction, so as to prepare dopamine modified MOF; reacting the dopamine modified MOF, EDOT and ferric salt in a solvent, and collecting the precipitate to prepare a nano filler; the preparation method comprises the following steps: jointly dispersing the double-bond functional polymer, active polypeptide, a photoinitiator and nano filler into a solvent, and carrying out photocuring cross-linking. The preparation condition is mild, gel forming is rapid, the method is simple, the prepared hydrogel has injectable performance and conductive performance, catalytic activity of natural enzyme can be simulated, active oxygen removal can be achieved in the early stage of myocardial ischemia, electrical conduction of infarction parts can be enhanced, and effective recovery of cardiac functions can be achieved.

The invention discloses an in-situ injectable bone repair adhesive material with a double-sequence osteogenesis promoting function as well as a preparation method and application of the in-situ injectable bone repair adhesive material. The preparation method comprises the following steps: 1) sequentially carrying out dual chemical grafting modification on hyaluronic acid by using methacrylic anhydride and dopamine hydrochloride to obtain a DA-HAMA biomacromolecule with UV curing and adhesion functions; (2) preparing a Ca < 2 + > physical cross-linked sodium alginate microsphere material loaded with an osteogenesis promoting drug by using a micro-fluidic system; and (3) preparing a DA-HAMA solution under dark and heating conditions, adding the vascular promoting factors and the drug-loaded microspheres when the DA-HAMA solution is cooled to room temperature, uniformly mixing, and carrying out centrifugal defoaming and irradiation sterilization to obtain the in-situ injectable bone repair adhesive material. The bone repair adhesive material has the functions of being degradable, injectable, capable of being adhered in a wet state, promoting blood vessels and promoting bone formation, and a brand new strategy is provided for bone defect repair.

The invention relates to the field of biomedical materials, in particular to a composite hydrogel microsphere with excellent cell adhesion performance and a preparation method and application thereof, and the preparation method comprises the following steps: (1) modifying silk fibroin with glutathione to obtain sulfydryl modified silk fibroin SF-GSH; (2) adopting methacrylic anhydride as a double-bond introduction reagent, introducing a methacrylate group on hyaluronic acid and forming a carbon-carbon double bond to obtain double-bond modified hyaluronic acid HA-MA; (3) mixing the sulfydryl modified silk fibroin SF-GSH with double bond modified hyaluronic acid HA-MA, and adding lactobionic acid to prepare a composite hydrogel microsphere precursor solution; and (4) preparing the composite hydrogel microsphere precursor solution into the composite hydrogel microsphere containing lactobionic acid. Compared with single hyaluronic acid hydrogel, the composite hydrogel microspheres prepared by the preparation method provided by the invention have good biocompatibility.

The invention discloses hyaluronic acid / alginic acid dual-network hydrogel as well as a preparation method and application thereof. The hyaluronic acid / alginic acid double-network hydrogel comprises a first network and a second network which are not crosslinked with each other, and the first network and the second network are distributed in the hydrogel in an interpenetrating manner; the first network comprises an ionic cross-linked network of alginic acid with cell adhesion sites and calcium ions, and the second network comprises a chemical cross-linked network formed by covalent cross-linking of methacrylic anhydride modified hyaluronic acid. The hyaluronic acid / alginic acid double-network hydrogel disclosed by the invention is short in curing time, supports three-dimensional spreading of cells and formation of capillary lumens, has good mechanical stability, and has good application prospects in the aspects of three-dimensional culture of the cells, tissue engineering construction and the like.

The invention relates to photo-crosslinking chitosan immobilized tannase, a preparation method thereof and application of the photo-crosslinking chitosan immobilized tannase in treatment of a tea extract. According to the invention, methacrylic anhydride is successfully grafted to the surface of chitosan to prepare UV cross-linked CS-MA, and then tannase is anchored on the CS-MA hydrogel through NHS to successfully complete immobilization. The immobilized tannase degrades methyl gallate, and the activity, stability and reusability of the enzyme can be improved. The immobilized tannase is more ideal for improving the pH value, color and clarity of tea soup compared with free enzyme, and the improvement on the pH value is particularly remarkable.

The invention discloses a high-performance double-slurry quick-setting grouting material and a preparation method thereof, and belongs to the field of building materials, the high-performance double-slurry quick-setting grouting material comprises a component A and a component B. The component A is base slurry, and the component B is a performance regulator of the component A; the component A is prepared from the following components in parts by weight: 30 to 35 parts of methacrylic anhydride gelatin modified sulphoaluminate cement, 5 to 8 parts of calcium powder, 0.25 to 0.45 part of lanthanum cerium oxide, 0.5 to 0.8 part of amino acid type surface activity exciting agent, 1 to 1.5 parts of polycarboxylic acid water reducing agent and water, and the water-cement ratio of the water to the modified sulphoaluminate cement is 0.35 to 0.45; and the component B is prepared from the following components: hydroxyethyl cellulose acrylamide modified bentonite, N-vinyl pyrrolidone / methacrylic acid / 2-hydroxy-3-methacryloyloxypropyl trimethyl ammonium chloride terpolymer, a double initiator, a cross-linking agent and deionized water. The bonding strength of the whole system of the grouting material can be enhanced, and the mechanical strength of the grouting material can also be improved.

The invention provides a chitosan-based hydrogel optical fiber material as well as a preparation method and application thereof, and belongs to the technical field of composite materials. Chitosan is modified by methacrylic anhydride to obtain a matrix solution of the hydrogel optical fiber, the reaction performance of the chitosan is enhanced, the modified chitosan and acrylamide are mixed, the mixture is irradiated by ultraviolet light to obtain the hydrogel fiber, then the hydrogel fiber is coated with a polyacrylamide solution, the chitosan with a high refractive index is used as a fiber core, and the hydrogel optical fiber is prepared. Acrylamide with excellent mechanical property is used as a coating layer, the problem of large light loss caused by insufficient refractive index of most of hydrogel is effectively avoided, the optical performance of the hydrogel optical fiber is improved, the prepared chitosan-based hydrogel is used as an optical fiber material, the biocompatibility and biodegradability of a brain-computer interface can be improved, and the application prospect is wide. The preparation method is simple and easy to operate.

The invention relates to a double-layer dressing for promoting wound healing and inhibiting scars and a preparation method and application thereof.The double-layer dressing comprises a hydrogel layer and a piezoelectric film layer compounded on the hydrogel layer, and the piezoelectric film layer is prepared by mixing an organic piezoelectric material, a ferroelectric material and an organic solvent and then conducting an electrostatic spinning technology; the hydrogel layer is formed by curing a methacrylic anhydride acellular extracellular matrix, methacrylic anhydride catechol chitosan and conductive nanoparticles under the action of a curing agent. The lower skin contact layer of the double-layer dressing is the hydrogel layer, so that the directional growth of fibroblasts is favorably induced; the upper dressing is a piezoelectric material composite ferroelectric material, is beneficial to piezoelectric stimulation re-induction function recovery after final skin repair and guidance of directional growth of wound cells, and has good skin wound induction repair and scar inhibition effects.

The invention provides a modified small intestinal submucosa which is a small intestinal submucosa grafted by methacrylic anhydride, and the grafting rate of the methacrylic anhydride is 40-90%. After cross-linking and curing, the modified small intestinal submucosa material provided by the invention has excellent biocompatibility, degradability and mechanical properties, can effectively promote revascularization and regulate immunoreaction, and has a wide application prospect as a tissue repair material.

The invention discloses a conductive microneedle patch for diabetes wound healing and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The conductive microneedle patch is of a nesting doll type double-layer structure formed by an inner layer and an outer layer, microneedles on the inner layer are constructed through a methacrylic anhydride modified gelatin template pouring method, and polyvinyl alcohol microneedles on the top layer are formed by the outer layer through a lining-template pouring method. Wherein the microneedle patch has remarkable conductivity and antibacterial property due to a conductive nano-material silver nanowire in the inner-layer microneedle, and the outer-layer microneedle is loaded with a hypoglycemic drug and a natural humectant betaine, so that the properties of moisturizing and continuously releasing drugs are achieved. The obtained microneedle patch can keep a wound moist environment and adjust a diabetes wound inflammation microenvironment, cell and blood vessel growth is promoted through exogenous electrical stimulation, and rapid healing of a diabetes wound can be effectively achieved by releasing a hypoglycemic drug.

The invention provides a methacrylic anhydride modified sclera extracellular matrix repair material and a curing method thereof, and belongs to the technical field of medical repair materials. The methacrylic anhydride modified sclera extracellular matrix repair material provided by the invention is prepared from the following raw materials: a methacrylic anhydride modified sclera extracellular matrix, methacrylated gelatin, a photoinitiator and a solvent. The sclera extracellular matrix modified by methacrylic anhydride is adopted, methacrylic anhydride contains methacrylic acid, double bonds can be introduced, the sclera extracellular matrix has the photo-initiation polymerization capacity, and in-situ gelling can be carried out at sclera and cornea damage positions under the action of a photoinitiator; the sclera extracellular matrix has good biocompatibility and histocompatibility, so that sclera matrix cells and corneal epithelial cells are adhered and proliferated, and the sutureless repair is realized; the methacrylated gelatin can provide a photo-crosslinking gelatin network under the action of a photoinitiator, so that cell adhesion and proliferation can be promoted, and repair is realized.

The invention discloses a preparation method of a 32P-loaded ultrathin flaky chitosan hydrogel applicator. The preparation method comprises the following steps: (1) preparing a chitosan aqueous solution; (2) dropwise adding methacrylic anhydride into the chitosan aqueous solution for reaction to obtain methylacryloylated chitosan; (3) preparing a methylacryloyl chitosan aqueous solution; (4) adding 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone into the methylacryloylated chitosan aqueous solution to obtain a chitosan hydrogel precursor solution, and (5) coating the inner wall of a mold made of a polytetrafluoroethylene material with a super-hydrophilic coating; (6) adding the Na2H32PO4 aqueous solution and the chitosan hydrogel precursor solution into a mold, stirring, and carrying out illumination curing; and (7) taking out the cured hydrogel and packaging the hydrogel between two layers of transparent adhesive tapes. According to the application, the ultrathin hydrogel applicator can be prepared, so that the blocking of the hydrogel material to rays is reduced, and the curative effect of the applicator is enhanced.

The invention discloses fish mucus-methacrylated gelatin hydrogel with lubrication and resistance reduction performance and a preparation method of the fish mucus-methacrylated gelatin hydrogel. The preparation process of the hydrogel comprises the following steps: firstly, preparing a methacrylated gelatin hydrogel (GelMA) precursor by utilizing an amidation reaction of methacrylic anhydride (MA) and gelatin (Gel), and then realizing gelation by utilizing hydrogen bond interaction of a fish mucus freeze-dried product (MC) and GelMA and photo-crosslinking of the GelMA precursor and polyethylene glycol diacrylate (PEGDA) under ultraviolet light of 365nm. The mucus-methylacryloylated gelatin hydrogel (MC-GelMA) is obtained after the mucus-methylacryloylated gelatin hydrogel is prepared. The hydrogel prepared by the preparation method disclosed by the invention has excellent swelling resistance and mechanical strength. Meanwhile, the hydrogel also has good lubrication and drag reduction performance, and can be used as a ship drag reduction material.

The invention discloses polysaccharide-based double-network hydrogel as well as a preparation method and application thereof. The polysaccharide-based double-network hydrogel comprises a first gel network and a second gel network interwoven with the first gel network, the first gel network is formed by crosslinking modified chitosan and modified hyaluronic acid through a Schiff base reaction, and the second gel network is formed by crosslinking modified chitosan and modified hyaluronic acid through a photopolymerization reaction; wherein the modified chitosan is chitosan modified by glycidyl methacrylate or chitosan which is coupled with a cartilage regeneration promoting substance and is modified by glycidyl methacrylate, and the modified hyaluronic acid is formylated hyaluronic acid modified by methacrylic anhydride. The hydrogel prepared by the invention has controllable aperture, proper swelling property and long-term stable drug sustained release effect, can effectively maintain the activity of mesenchymal stem cells, has good in-vivo biocompatibility, and can significantly promote cartilage regeneration.

The invention discloses a GelMA hydrogel composite material as well as a preparation method and application thereof, and belongs to the technical field of material preparation. A micro-needle-shaped GelMA hydrogel composite material (GelMA MNs) is prepared by grafting an anhydride functional group of methacrylic anhydride to a side chain amino group of gelatin, then doxorubicin and an aPD-L1 inhibitor are loaded into the GelMA MNs, a double-drug delivery system is established, and the prepared aPD-L1 / DoxatGelMA MNs can reduce skin injury to the maximum extent, and meanwhile, the aPD-L1 / DoxatGelMA MNs have a good application prospect. Recognition and killing of immune cells on melanoma cells are greatly started, immunogenic cell death (ICD) is promoted, and a new thought is provided for overcoming limitation of ICIs.

The present invention pertains to the technical field of materials. Disclosed are a polymer composite hydrogel and a preparation method therefor, and a tooth desensitization material. The polymer composite hydrogel consists of a GelMA hydrogel and BP nanosheets composited inside the GelMA hydrogel, wherein the GelMA hydrogel is prepared by crosslinking gelatin methacryloyl via double bonds under the action of a photoinitiator. Under the photothermal effect, the polymer composite hydrogel of the present invention can enhance the resistance of dentin to external stimuli and promote the formation of stable and effective occlusion of dentinal tubules. Moreover, the occlusion effect on the dentinal tubules exhibits acid resistance, corrosion resistance, and stability, preventing the possibility of short-term reopening of the occluded dentinal tubules. This is of great significance for the treatment of occluded dentin hypersensitivity (DH).

The invention relates to the technical field of medical interventional therapy materials, and particularly discloses a preparation method of sodium hyaluronate embolism microspheres and the prepared embolism microspheres. The preparation method of the sodium hyaluronate embolization microspheres comprises the following steps: S1, adding methacrylic anhydride, N, N-dimethylformamide and sodium hydroxide into a sodium hyaluronate aqueous solution, reacting, adding sodium chloride, reacting, adding absolute ethyl alcohol, precipitating, filtering to remove filtrate, cleaning and drying; s2, preparing a mixture of methacrylated sodium hyaluronate, a monomer with negative charges, an initiator and water into a water phase; a mixture of oil soluble liquid and a dispersing agent is used as an oil phase; and under the protection of nitrogen, carrying out reverse suspension polymerization. According to the preparation method of the sodium hyaluronate embolization microspheres, the methylacryloylated sodium hyaluronate and the negatively charged monomer are copolymerized to introduce the negatively charged group, so that the microspheres can effectively load chemotherapeutic drugs, and the compression elasticity is high.

The invention relates to the technical field of bone tissue materials, and discloses a metal-doped biomimetic mineralized dECM cryogel and a preparation method and application thereof.The preparation method of the cryogel comprises the steps that firstly, hyaluronic acid and methacrylic anhydride are mixed in a solvent, the pH value is adjusted, a reaction is conducted, and methacrylic acid hyaluronic acid is obtained; then under the condition of a photoinitiator, mixing the methacrylic acid hyaluronic acid with an acellular extracellular matrix, and performing freezing and crosslinking treatment to obtain the cryogel, and finally, soaking the cryogel in a mineralization solution containing Ca < 2 + > and / or Mg < 2 + >, adding a buffer solution containing phosphite ions, incubating, and washing to obtain the crystal gel. The biomimetic mineralized dECM cryogel prepared by the method disclosed by the invention is good in ion release stability, can significantly improve the migration ability of HUVECs, contributes to promoting proliferation of rMSCs and improving the osteogenic differentiation potential of the rMSCs, and can be used for regeneration and repair of bone defects.

The invention provides a GelMA microneedle patch loaded with chromogenic gold nano bipyramids and a preparation method thereof.The method comprises the steps that citric acid, chloroauric acid and a CTAC solution are mixed, then sodium borohydride is added, and a seed solution is obtained through stirring and standing; mixing chloroauric acid, silver nitrate, hydrochloric acid and CTAB (cetyltrimethyl ammonium bromide), and sequentially adding ascorbic acid and the seed solution to obtain a growth solution; carrying out centrifugation, morphology regulation and chemical etching purification to obtain a chromogenic gold nano bipyramid solution; the preparation method comprises the following steps: reacting gelatin with methacrylic anhydride in PBS, dialyzing and freeze-drying to obtain GelMA; dissolving GelMA in the gold nanoparticle bipyramid solution, and adding a photoinitiator LAP to obtain a mixed precursor solution; and the GelMA microneedle patch loaded with the gold nano bipyramid is prepared through mold casting, centrifugation, ultraviolet light crosslinking and drying demolding. The microneedle extraction and gold nano bipyramid color development integrated scheme is initiated, blood sampling is not needed, the dermis interstitial fluid is extracted in a minimally invasive mode through the microneedle, detection pain and trauma are reduced, the cross infection risk of blood treatment is avoided, and the microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle

The invention discloses a conductive hydrogel for promoting bone growth and a preparation method and application thereof.The preparation method comprises the steps that 1, a methacrylic anhydride gelatin hydrogel prepolymer solution is prepared, a photoinitiator is dissolved in deionized water, and a solution with the mass-volume concentration of 0.003-0.005 g / mL is prepared; methylacrylic anhydride gelatin Gelma is dissolved in the solution, and a methylacrylic anhydride gelatin hydrogel prepolymerization solution is prepared; (2) preparing a CuNPs dispersion liquid; (3) preparation of photo-crosslinking hydrogel: mixing the methylacrylic anhydride gelatin prepolymerization liquid and the CuNPs dispersion liquid to prepare a methylacrylic anhydride gelatin copper nanoparticle conductive hydrogel mixed liquid; in the mixed solution, the massic volume concentration of the methacrylic anhydride gelatin is kept at 0.05 g / mL, the concentration of the copper nanoparticles is 10 mmol / L-40 mmol / L, bubbles are removed, the mixed solution is added into a mold, ultraviolet irradiation is performed, and the photo-crosslinking hydrogel can be prepared. The gel has good conductivity, pore structure, biocompatibility and mechanical property, and has a good osteogenesis promoting effect. The preparation process is simple, and the repeatability is high.

The invention discloses a high-adhesion enzymatic hydrogel for promoting vascular intima repair and a preparation method of the high-adhesion enzymatic hydrogel, and belongs to the technical field of hydrogel preparation. The preparation method comprises the following steps: S1, carrying out double-bond modification on hyaluronic acid by utilizing methacrylic anhydride, and then cross-linking with dopamine to prepare dopamine modified double-bond hyaluronic acid; s2, deoxidizing the hyaluronic acid solution, adding a cross-linking agent and tyramine hydrochloride, and reacting in an acidic environment to prepare tyramine modified hyaluronic acid; s3, mixing the dopamine modified double-bond hyaluronic acid, the tyramine modified hyaluronic acid and deionized water to prepare a hydrogel precursor liquid; and S4, carrying out enzymatic reaction to obtain the hydrogel. The method designed by the invention is simple in process, no extra photocuring equipment needs to be added, and the constructed hydrogel is similar to an extracellular matrix. And the coating can be coated into different shapes to adapt to different environments. The gel is formed through enzymatic reaction, and the gel is rapid in gel forming, good in tissue adhesion, good in biocompatibility and suitable for being applied to vascular repair.

The invention discloses a plant-based deodorant and a preparation method thereof, and relates to the technical field of high polymer materials. When the plant-based deodorant is prepared, beta-cyclodextrin reacts with paratoluensulfonyl chloride and then reacts with ethylenediamine, and amino modified cyclodextrin is prepared; the plant-based extract is subjected to inclusion with amino modified cyclodextrin, and a cyclodextrin inclusion compound is prepared; the preparation method comprises the following steps: reacting hydroxyethyl cellulose with methacrylic anhydride to prepare double-bond modified cellulose; carrying out hydrothermal reaction on titanium sulfate, the carboxylated multi-walled carbon nanotube, ammonium fluoride and cerous nitrate to prepare a co-doped titanium dioxide / carbon nanotube; coating the co-doped titanium dioxide / carbon nano-tubes with oleic acid to prepare modified nano-particles; and reacting the double-bond modified cellulose with the cyclodextrin inclusion compound, and then copolymerizing with the modified nanoparticles and the comonomer to obtain the plant-based deodorant. The prepared plant-based deodorant has the advantages of good deodorization effect, slow release and photocatalytic degradation.

The invention discloses injectable hydrogel with a myocardial tissue repairing function as well as a preparation method and application of the injectable hydrogel, and relates to the technical field of medical materials. The preparation method of the injectable hydrogel comprises the following steps: carrying out a reaction on deferoxamine and DSPE-PEG-NHS, so as to obtain DSPE-PEG-DFO; the preparation method comprises the following steps: wrapping carvedilol with DSPE-PEG-DFO under the hydrophilic and hydrophobic action, so as to obtain a drug-loaded nano-micelle; the preparation method comprises the following steps: reacting a polymer containing amino or hydroxyl with methacrylic anhydride to obtain a methylacryloyl modified polymer; reacting the carboxyl-containing polymer with cysteamine hydrochloride to obtain a sulfydryl modified polymer; mixing the drug-loaded nano-micelle, the methylacryloyl modified polymer and the sulfydryl modified polymer, and reacting under ultraviolet radiation to obtain the injectable hydrogel. The hydrogel disclosed by the invention is good in biocompatibility, can release deferoxamine and carvedilol in a ladder manner, is matched with a myocardial repair process, and realizes a function of repairing damaged myocardial tissues.

The invention discloses a novel oral delivery microcarrier with an intestinal adhesion function and a preparation method thereof, and the preparation method specifically comprises the following steps: preparing methacrylic anhydride gelatin, o-nitrobenzaldehyde group gelatin, a photoinitiator, a color indicator and a drug to be loaded into a mixed solution, and preparing microspheres by using a microfluidic instrument, and irradiating with an ultraviolet light source until curing to obtain the novel oral delivery microcarrier with the intestinal adhesion function. According to the invention, the limitation of the existing traditional single material is overcome, the compounding ratio of GelMA to GelNB is optimized, and the stability and biological activity of the microspheres are ensured; drug molecules are successfully and uniformly entrapped in the microspheres through an advanced microsphere preparation microfluidic technology; the targeting delivery efficiency of the medicine is improved, the healing and regeneration of tissues are promoted, and a new strategy and a new tool are provided for disease treatment and tissue engineering application.

The invention provides antibacterial silk fibroin hydrogel as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The antibacterial silk fibroin hydrogel provided by the invention is prepared from the following components: modified silk fibroin and copper montmorillonite, the modified silk fibroin is at least one of glycidyl methacrylate modified silk fibroin, methacrylic anhydride modified silk fibroin, isocyanate ethyl methacrylate modified silk fibroin and norbornene anhydride modified silk fibroin, and chemical crosslinking is formed among molecules of the modified silk fibroin. The copper montmorillonite and the modified silk fibroin form physical crosslinking through hydrogen bonds, and the mass ratio of the modified silk fibroin to the copper montmorillonite is 100: (1-10). The antibacterial silk fibroin hydrogel provided by the invention has good biological safety, long-acting antibacterial property, electrical conductivity and good mechanical property.

The invention discloses a decellularized amniotic membrane matrix composite scaffold, a preparation method thereof and application of the decellularized amniotic membrane matrix composite scaffold in orbital bone defect repair, and relates to the technical field of biomedical engineering and biomedical materials. In order to solve the limitation of an existing orbit implant material, methacrylic anhydride (MA) is grafted to HAM, methacrylate HAMMA hydrogel is synthesized, and then the decellularized amnion matrix composite scaffold (HAMMA / HAP / BMP-2) is prepared by combining a simulated body fluid biomimetic mineralization method with 3D printing and ultraviolet curing. The composite scaffold material prepared by the invention can regulate the release of BMP-2 and maintain the effective drug concentration of a bone defect area so as to regulate the immune microenvironment of an early bone defect repair area, collect osteoblasts and accelerate the repair of bone defects.

The invention provides an analgesic microneedle patch based on 3D printing and a preparation method and application thereof, and belongs to the technical field of biomedical materials.The preparation method of the hydrogel microneedle patch based on 3D printing comprises the following steps that 1, cold water fish skin gelatin is dissolved in DPBS, and a gelatin solution is obtained; (2) mixing methacrylic anhydride and gelatin liquid to generate polymerization reaction, so as to obtain a hydrogel material; and 3) loading an anesthetic and analgesic drug on the hydrogel material, and printing by a photocuring 3D printing method to obtain the hydrogel microneedle patch. The 3D printing technology is combined with a microneedle which is a dysmenorrhea-free skin drug delivery system, local anesthetics are loaded into the microneedle patch, on the basis of painless drug delivery, the drug delivery efficiency is improved, the drug onset time is shortened, and then the effect of effectively, rapidly and safely playing a local anesthesia role is achieved.

The invention belongs to the technical field of biomedical materials, and particularly relates to a hydrogel dressing based on traditional Chinese medicine polysaccharide and a preparation method of the hydrogel dressing. The hydrogel dressing based on the traditional Chinese medicine polysaccharide is prepared from the following raw materials in parts by weight: 10 to 15 parts of gastrodia elata polysaccharide, 8 to 12 parts of rhizoma bletillae polysaccharide, 5 to 8 parts of astragalus polysaccharide, 1 to 3 parts of a nano-silver-baicalin compound, 2 to 3 parts of methacrylic anhydride, 0.02 to 0.05 part of VEGF, 0.4 to 1 part of PLGA, 0.1 to 0.3 part of Irgacure 2959, 20 to 30 parts of a 5% calcium chloride solution, 0.1 part of polyvinyl alcohol and 100 to 170 parts of PBS. A triple gel network structure is constructed through photocuring, ionic crosslinking and an emulsification-solvent evaporation method, so that the function integration of controlled release, antibiosis and vascular regeneration promotion is realized, and the clinical treatment requirements of complex wounds are effectively met.

The invention discloses a magnetic resonance visual hydrogel material capable of dynamically monitoring tissue reconstruction, and belongs to the technical field of tissue repair materials. The material comprises a modified gadolinium contrast agent, a cross-linking composition, methacrylic anhydride gelatin, a cross-linking agent and a photoinitiator, the modified gadolinium contrast agent comprises sodium alginate, ammonium alginate and trivalent gadolinium ions, and the cross-linking composition comprises carboxyl modified polyvinyl alcohol, sulfhydrylated hyaluronic acid and chitosan. According to the invention, trivalent gadolinium ions are embedded into a matrix of sodium alginate and ammonium alginate to form stable nanoparticles or a compound, so that the sensitivity is improved, an imaging window is prolonged, the degradation process of a stent material can be continuously tracked, and the mechanical property and the stability of the hydrogel are improved through a cross-linking composition; the service life and the preservation time of the hydrogel are prolonged.