296 results about "Methacrylic anhydride" patented technology

The invention discloses a preparation method of self-adhesion electroconductive hydrogel and belongs to the technical field of biomaterial preparation. The preparation method includes following steps: introducing double bonds on biomacromolecules through methyl acrylic anhydridization to obtain double-bond biomacromolecules; preparing a polydopamine-functionalized electroconductive nano material, and polymerizing the double-bond biomacromolecules and the polydopamine-functionalized electroconductive nano material through photo-initiated free radicals to obtain the self-adhesive electroconductive hydrogel. With reference to adhesiveness of mussel-imitated materials, phenolic hydroxyl functional group with adhesiveness is introduced, and regulation of self-adhesion performance of the hydrogel is realized by changing content of the phenolic hydroxyl functional group. In addition, the self-adhesion electroconductive hydrogel prepared by the method has high electroconductivity, mechanical performance and biocompatibility, and application range of the hydrogel is widened. The preparation method has the advantages of being simple and easy to implement, controllable in polymerization process, high in polymerization speed and low in energy consumption.

The invention discloses a preparing method for infrared response high-strength hydrogel for cartilago articularis repair. The preparing method mainly includes the operation steps that polysaccharide or protein is modified in a double bond forming mode through methacrylic acid, methacrylic anhydride and methacrylate substances, so that the polysaccharide or the protein has a double-bond group in a side chain and is capable of being polymerized to form the hydrogel. Under the action of an initiator and a cross-linking agent, N-isopropyl acrylamide and the polysaccharide or the protein modified in the double bond forming mode form the double-network high-strength hydrogel through in-situ polymerization. The prepared infrared response high-strength hydrogel is high in biocompatibility and excellent in mechanical property and has good application prospects in the field of cartilago articularis repair.

The invention discloses a method for preparing a metal-organic framework material film, and belongs to the technical field of preparation of inorganic-organic hybrid films. The method comprises the following specific steps: enabling a ZrC14 solution to react with a 2-aminaterephthalic acid solution to prepare UiO-66-NH2; adding methacrylic anhydride to obtain modified UiO-66-NH2; adding modified UiO-66-NH2 in an acrylate organic monomer, adding a photoinitiator, and irradiating under UV light to obtain the metal-organic framework material film. The method is a simple and fast MOF film processing method, self-supported MOFs hybrid films of different polymer systems are synthetized, MOFs particles and the polymer systems are bonded, and the MOF particles are dispersed uniformly in the polymer systems. The method is simple, fast, and low in raw material cost, and has industrialized advantages.

The invention discloses a photo-curing monomer with ortho-phenolic hydroxyl strucutre, a preparation method and bond thereof. In the formula I monomer, the compound which has crylic acid at two ends or crylic acid double bond at one end and methylacrylic acid double bond at the other end is added to the amidogen of dopamine and derivant thereof through a Michael addition method to form corresponding secondary amine or tertiary amine. In the formula II monomer, the double bond is introduced by (methyl) acrylic anhydride, (methyl) crylic acid and (methyl) acryloyl chloride. The photopolymerization bond comprises the following components by weight percent: 20 to 99.9 percent of the compound with the ortho-phenolic hydroxyl structure, 0 to 70 percent of activated thinner and / or solvent, 0.1 to 10 percent of evocating agent and 0 to 60 percent of natural macromolecule or modified outcome thereof. The bond can be bonded in dry state and wet state, is a quick and long lasting bond, realizes the organic bond of biological bond and photopolymerization, has the advantages of low manufacture cost, simple and convenient operation and high productivity and has extensive application foreground in the biological bond field.

The invention provides a photo-crosslinking material which is composed of a methacryliated material containing -OH and -NH2 groups. The photo-crosslinking material is prepared by the following method: 1) the material containing -OH and -NH2 groups is dissolved; and 2) a solution obtained and methacrylic anhydride react for at least 16 h. The invention also provides hydrogel formed by the photo-crosslinking material. The hydrogel is prepared by the following method: 1) a light initiator is dissolved in a transparent solution of the photo-crosslinking material when protected from light; and (2) a solution obtained is illuminated. The hydrogel can be used as a repairing material of wounded tissues.

The invention provides a method for preparing cartilage with a high mechanical property based on 3D bioprinting. The method comprises the following steps: preparing silk fibers, preparing a gelatin solution, preparing ciological ink containing cells, carrying out 3D cartilage printing, performing cell culture and differentiation on a printed tissue to form the cartilage. The method solves the problems of polarization of 2-dimension culture for cells and high body and surface ratio of cells through the 3D bioprinting technology; sodium alginate and calcium ions form ionic bonds, and gelatin and methacrylic anhydride form covalent bonds, so that the 3D bioprinted cartilage is good in mechanical property and is not easily broken; the gelatin has biocompatibility and degradability of normal biological material hydrogel, has bioactivity, and is good for cell growth and differentiation and realization of cell functions.

The invention discloses a host-guest supramolecular hydrogel, and a preparation method and an application thereof. The host-guest supramolecular hydrogel is prepared through initiating the copolymerization of free radicals by host-guest supramolecule and methacrylic anhydride gelatin (GelMA) under an ultraviolet light (UV ) condition, and has good mechanical strength and biocompatibility. The host-guest supramolecule is prepared through the host-guest coupling of acryloyloxyethyl isocyanate-modified beta-cyclodextrin (beta-CD) and allyl 1-bromoadamantane. Compared with pure GelMA hydrogel, thehost-guest supramolecular hydrogel has the advantages of good mechanical properties, control of the mechanical strength of the hydrogel by adjusting the concentration of the host-guest supramolecule,and good biocompatibility. The host-guest supramolecular hydrogel also has the advantages of simple preparation process, mild conditions, effective improvement of the mechanical strength, and good application prospect in the field of tissue repair engineering.

The invention discloses thermosensitive hydrogel loaded with copper metal organic skeleton nanoparticles and a preparation method of the thermosensitive hydrogel. The preparation method comprises thesteps that an amino group on gelatin and methacrylic anhydride are subjected to acylation reaction to synthesize methylacrylic esterified gelatin with a branch containing a carbon-carbon double bond,the carbon-carbon double bond on the branch and N-isopropyl acrylamide are subjected to free radical polymerization, and methylacrylic esterified gelatin-g-poly N-isopropyl acrylamide is prepared; 1,3,5-benzene tricarbonic acid and copper acetate monohydrate are used for preparing copper-based MOF nanoparticles; the copper-based MOF nanoparticles are added to a methylacrylic esterified gelatin-g-poly N-isopropyl acrylamide solution, and a product is prepared. Copper ions are embedded in the biodegradable thermosensitive hydrogel in the form of HKUST-1 NPs, controlled slow release of the copperions is achieved, cytotoxicity is effectively reduced, migration of in-vitro dermal cells is promoted, angiogenesis is induced, and wound healing is promoted.

According to the present invention, a method for manufacturing a liquid discharge head includes the steps of depositing a solid layer for forming a flow path on a substrate on which an energy generating element is arranged to generate energy that is used to discharge liquid, forming, on the substrate where the solid layer is mounted, a coating layer for coating the solid layer, forming a discharge port used to discharge a liquid, through a photolithographic process, in the coating layer formed on the solid layer, and removing the solid layer to form a flow path that communicates with the energy element and the discharge port, whereby a material used for the coating layer contains a cationically polymerizable chemical compound, cationic photopolymerization initiator and a inhibitor of cationic photopolymerization, and whereby a material of the solid layer that forms a boundary with a portion where the discharge port of the coating layer is formed contains a copolymer of methacrylic anhydride and methacrylate ester.

The invention discloses photo-crosslinked recombinant collagen hydrogel, a preparation method and application of the photo-crosslinked recombinant collagen hydrogel in 3D biological printing. The preparation method comprises the following steps: adding methacrylic anhydride into a phosphate solution of recombinant collagen, fully stirring and reacting to obtain methacrylic anhydride modified recombinant collagen, dissolving the modified recombinant collagen into a culture medium, adding a photoinitiator, uniformly mixing, then carrying out photoreaction, crosslinking and curing to obtain the photo-crosslinked recombinant collagen hydrogel. A recombinant collagen hydrogel precursor solution is placed in a mold or serves as 3D printing ink, and subjected to photoreaction, crosslinking and curing to prepare a tissue engineering scaffold or a 3D printing finished product; and cells can be added into the hydrogel precursor solution to prepare a tissue engineering scaffold or a 3D printing finished product loaded with the cells. The disclosed hydrogel is good in physical and chemical properties, good in biocompatibility, stable in quality and good in formability, can be prepared into anysize and shape, and is suitable for large-scale industrial production.

The invention belongs to the technical field of hydrogel materials and discloses polypyrrole nano composite conductive hydrogel and a preparation method thereof. The method comprises the following steps of a, using pyrrole as a monomer and dopamine as a dopant for in-situ chemical oxidative polymerization to prepare a polypyrrole nano material; b, uniformly mixing the polypyrrole nano material, modified gelatin and a photoinitiator in a solvent, and carrying out ultraviolet light initiation polymerization to obtain the polypyrrole nano composite conductive hydrogel. The modified gelatin is obtained by modifying gelatin with methacrylic anhydride. According to the hydrogel material, by modifying polypyrrole with dopamine, the polypyrrole nano material is obtained; the polypyrrole nano material can be well dispersed in a hydrogel matrix material and meanwhile can stably exist under the hydrogen bond action with hydrogel molecules. The prepared conductive hydrogel has excellent conductivity and stable chemical property, and the mechanical property of the hydrogel is improved.

The invention discloses a preparation method of composite gel micro-spheres. The preparation method comprises the following steps: performing modification treatment on silk fibroins to obtain a methacrylate anhydride silk fibroin solution; adding a methacrylate anhydride bio-polymerized molecular solution and a photo-initiator to obtain a pre-polymer solution; preparing composite micro-droplets bythe pre-polymer solution, performing radiation treatment with blue light to obtain the silk fibroin protein and the bio-polymerized molecule composite gel microspheres. The composite gel microsphereswith high mechanical property and good biocompatibility can be prepared by the method. The invention further discloses a cell coating method based on the preparation method; composite gel microsphere-coated cells are small in damage and low in toxicity and can be obtained with good survival and proliferation. The invention also discloses the composite gel micro-spheres prepared by the method andapplication thereof to cell 3D culture, 3D printing and use as biomedical materials; the composite gel micro-spheres have a wide application to the biomedical field through good biocompatibility and mechanical property.

The invention belongs to the technical field of macromolecular biological materials, and provides a macromolecular composite self-healing hydrogel based on a molybdenum disulfide nanosheet and a preparation method thereof. The macromolecular composite self-healing hydrogel is prepared by performing photo-initiation clicking reaction in a molybdenum disulfide nanosheet water solution; the clicking reaction is generated by unsaturated double bonds in methacrylic anhydride gelatin and sulfydryl in four-branched sulfydryl polyvinyl alcohol under the radiation action of ultraviolet light. The self-healing property is realized by the material utilizing the photo-thermal conversion characteristic of the molybdenum disulfide nanosheet to heat the hydrogel under the radiation action of near-infrared light. The macromolecular composite self-healing hydrogel has the advantages that the nanometer two-dimensional material, namely the molybdenum disulfide nanosheet, is applied into the field of self-healing materials, and the quick healing characteristic of the material under the light stimulation condition is realized.

The invention discloses a chitosan derivative with cross-linking polymerization and containing drug ligand. The chitosan derivative is a grafted substance formed by grafting a methacrylic acid substance and beta-cyclodextrin on a chitosan main chain, wherein the beta-cyclodextrin is grafted on the chitosan main chain through epoxy chloropropane; and the methacrylic acid substance is hydroxyethyl methylacrylate or methacrylic anhydride. The chitosan derivative provided by the invention has the beneficial effects that the processibility is good, and the controlled release of hydrophobic drugs can be realized; a hydrogel corneal contact lens prepared by the chitosan derivative has the advantages that the transparency is good, the water content, the water-retaining property and protein absorption capability are balanced, and the loading capability and the controlled release capability to the drugs are better than those of the existing material obviously. The chitosan derivative disclosed by the invention has the advantages that the synthetic route is simple, the operability is strong and the social and economic benefits are larger.

The invention discloses GelMA-oxidized dextran double-network hydrogel and a preparation method thereof. The preparation method comprises: 1) preparing oxidized dextran: reacting a dextran solution with an oxidizing agent to obtain oxidized dextran, 2) preparing methacrylated gelatin: reacting a gelatin solution with methacrylic anhydride to obtain a methacrylated gelatin, and 3) preparing double-network hydrogel: mixing the oxidized dextran solution with the methacrylated gelatin solution, then mixing the mixture with a photoinitiator solution to obtain a mixed solution, then standing the mixture to form gel, and carrying out ultraviolet irradiation on the obtained gel to obtain the GelMA-oxidized dextran double-network hydrogel. The photo-crosslinking double-modified GelMA-oxidized dextran double-network hydrogel is stable in structure and good in mechanical property, the preparation raw materials are safe and environmentally friendly, the preparation method is simple, the reaction conditions are mild, and the wide application prospect is achieved.

The invention belongs to the field of biomedical materials and discloses dopamine-mediated polypyrrole conductive hydrogel and a preparation method thereof. The preparation method includes: dissolvingmethacrylic acid-modified gelatin and an acrylamide monomer in a phosphate buffered solution, adding a photoinitiator, triggering polymerization in ultraviolet to obtain modified gelatin-polyacrylamide hybrid hydrogel, soaking the modified gelatin-polyacrylamide hybrid hydrogel in a water solution containing a pyrrole monomer and dopamine hydrochloride, dropwise adding a peroxidant, and stirringto allow reacting to obtain the dopamine-mediated polypyrrole conductive hydrogel. The dopamine-mediated polypyrrole conductive hydrogel provided herein is prepared by growing pyrrole in situ on hydrogel through dopamine mediation, has the characteristics of both hydrogels and conductive polymers, has the advantages of good biocompatibility and excellent and stable electrical properties, and is expectedly applicable to the tissue engineering repair fields, such as neural restoration, cardiac muscular tissue regeneration, and skin restoration.

The invention relates to a thermo-sensitive and long-acting antibacterial arginine-group hydrogel dressing and a preparation method thereof. Antibacterial drugs are distributed on the surface of and inside hydrogel. The preparation method comprises steps as follows: L-arginine and sodium bicarbonate are dissolved in distilled water, methacrylic anhydride is dropwise added at 0 DEG C, the mixture is stirred, the pH of the solution is adjusted to be 7.4, the solution is filtered and washed, and a modified arginine monomer (M-Arg) is acquired; two antibacterial drugs, namely, A and B, are combined with an N-isopropylacrylamide monomer and a modified arginine monomer with chemical grafting and drug preloading methods, thermo-sensitive and long-acting antibacterial arginine-group hydrogel is obtained and combined with a matrix, and the thermo-sensitive and long-acting antibacterial arginine-group hydrogel dressing is obtained. Raw materials have good biocompatibility, and the obtained hydrogel dressing has thermosensitivity, can resist bacteria and protein absorption, has a long-acting antibacterial function and is suitable for wound protection and treatment of burns, scalds and traumatic injuries.

The invention provides a piezoelectric composite dressing for promoting peripheral nerve repair and wound healing and loading exosome of traditional Chinese medicine and a preparation method. The preparation method comprises the following steps that gelatin and methacrylic anhydride react to prepare methacrylate gelatin; the exosome of the Chinese medicine is prepared through differential centrifugation;a conductive pregel system is prepared through the recombination of gold nanorods, the exosome and the methacrylate gelatin; and the conductive pregel and a polyvinylidene fluoride ethylene piezoelectric thin film arerecombined, and the piezoelectric composite dressing is prepared. The dressing prepared by the preparation method is a novel dressing for piezoelectric stimulation composite medicine action, polyvinylidene fluoride ethylene produces electricity potential to stimulate a wound area through the conductive gel, wound healing and nerve regeneration are promoted with the exosomeof the traditional Chinese medicine, and the problems in the current wound repair and nerve repair process can be effectively solved. Therefore, the composite dressing has a wide application value inthe treatment of trauma and nerve repair.

The invention provides traditional Chinese medicinal exosome-loaded compound dressing with effects of promoting wound healing and regeneration and a preparation method of the compound dressing. The preparation method comprises the following steps: preparing methacrylate esterified gelatin by a reaction of gelatin and methacrylic anhydride; preparing a traditional Chinese medicinal exosome throughdifferential centrifugation; combining the prepared exosome with the methacrylate esterified gelatin, adding SDF-1 and photosensitizer into the mixture to prepare a pre-gel system; and forming the compound dressing through ultraviolet crosslinking. The dressing is novel photosensitive dressing of a compound traditional Chinese medicinal exosome, the compound can be formed through ultraviolet crosslinking in vitro or in-situ crosslinking on a wound after the pre-gel system is formed. The compound dressing is quick in crosslinking, is simple and convenient, can be used for effectively protectinga wound area, inducing migration of mesenchymal stem cells and promoting wound heating and regeneration with the traditional Chinese medicinal exosome, and can effectively solve the problem in current wound healing and regenerating process. Therefore, the compound dressing has a wide application value in wound healing and regeneration.

The present invention relates to fluorine and silicon containing copolymer resin and paint. The composition includes fluorinated compound in 1-30 wt%, silane 2-8 wt% and alkenyl containing compound 62-97 wt%. The fluorinated compound is acrylic fluoro resin obtained through the reaction of fluorinated alcohol and acryloyl chloride or acrylic anhydride; the silane is one containing alkenyl group or containing carbon functional group with oxygen atom; and the alkenyl containing compound methacrylic acid, methacrylate, vinyl carboxylate and alkenyl containing benzene compound. The present invention has the advantages of fluorinated acrylic acid copolymer and silicon containing acrylate copolymer paint and the paint is excellent single component paint.

The invention discloses a preparation method of vinyl collagen. The method includes: firstly dissolving collagen; and then conducting graft modification on the collagen. The invention employs methacrylic anhydride for graft modification of the collagen, the operation is simple, the reaction time is short, and the reaction conditions are milder. The equipment and medicines involved in the invention have the characteristics of low equipment requirements, low cost, and strong operability. The vinyl collagen prepared by the method provided by the invention lays the foundation for film forming crosslinking of collagen itself, graft crosslinking of other reactive high polymer materials and the like.

The invention belongs to the technical field of regenerative medical materials, and discloses hyaluronic acid supramolecular hydrogel for three-dimensional culture of chondrocytes, and preparation andapplication of the hyaluronic acid supramolecular hydrogel. The method comprises the following steps: with water as a reaction medium, carrying out a copolymerization reaction on methacrylic anhydride modified hyaluronic acid, a double-bond functionalized ureido pyrimidinone functional monomer and 2-(2-methoxyethoxy)ethyl methacrylate under the action of an initiating system, and carrying out subsequent treatment to obtain the hyaluronic acid supramolecular hydrogel. The hydrogel is used in the field of cartilage tissue engineering, and is used for three-dimensional culture of chondrocytes and preparation of cartilage repair materials. The hydrogel disclosed by the invention has sol-gel conversion and self-repairing properties, uniform dispersion of the chondrocytes can be easily realizedby sol at a low temperature, and the sol can undergo phase change to form gel after being conveyed to a cartilage injury part, so in-situ fixation of the chondrocytes is realized; and the formation of a dynamic three-dimensional microenvironment of the hydrogel is beneficial for the maintenance of chondrocyte functions and the secretion of cartilage characteristic matrixes.

The present invention discloses a gene carrier and the preparation method thereof. Chondroitin sulfate (CS) is reacted with methacrylic anhydride (MA) to form chrondroitin sulfate-methacrylate (CSMA), which is further covalently bound with polyethylenimine (PEI) via the Michael addition to produce a CSMA-PEI gene carrier. The CSMA-PEI gene carrier can effectively reduce the cytotoxicity of PEI and enhance the transfection efficiency of PEI.

The invention relates to a self-adhesive microneedle patch capable of swelling rapidly and a preparation method thereof. The self-adhesive microneedle patch and the preparation method thereof have theadvantages that first modifying agents (mixtures of one or more of acrylic anhydride and methacrylic acid) are used for modifying hydrophilic polymers to composite acrylate composite hydrophilic polymers; a secondary reaction between catechol-type compounds and the acrylate composite hydrophilic polymers is completed to composite catechol modified acrylate composite hydrophilic polymers with highbiocompatibility; when the hydrophilic polymers are prepared into the microneedle patch, the microneedle patch has high biocompatibility; the microneedle patch can absorb liquid quickly to swell, andis not blocked by skin tissues during absorption; due to the action of catechol groups, the microneedle patch can be adhered to the skin surface, additional adhesive plasters or tapes are not neededfor fixation, and accordingly, time, labor and cost are saved.

The invention relates to a 3D-printed degradable macromolecular scaffold and photo-crosslinked hydrogel composite scaffold. The 3D-printed degradable macromolecular scaffold and photo-crosslinked hydrogel composite scaffold comprises a 3D-printed degradable macromolecular scaffold, wherein the interior of the 3D-printed degradable macromolecular scaffold comprises photo-crosslinked hydrogel with high substitution degree and photo-crosslinked hydrogel with low substitution degree, the photo-crosslinked hydrogel with high substitution degree is crosslinked with the photo-crosslinked hydrogel with low substitution degree, and preferably, a polycaprolactone (PCL) scaffold and methacrylic anhydride gelatin (GelMA) with different substitution degrees are crosslinked and compounded. In the composite scaffold, the 3D-printed degradable macromolecular scaffold has good mechanical properties; the photo-crosslinked hydrogel with high substitution degree has high crosslinking degree, can form a fiber network and micropores, and well supports cells; and the photo-crosslinked hydrogel with low substitution degree has multiple active sites, is beneficial to cell adhesion growth, and can adsorb alarge amount of nutrient solution. Through cooperation of the 3D-printed degradable macromolecular scaffold, the photo-crosslinked hydrogel with high substitution degree and the photo-crosslinked hydrogel with low substitution degree, the composite scaffold is suitable for cell growth and vascularization from an inner layer to an outer layer, and when the composite scaffold is used for medical human body repair, scaffold integration is realized to promote regeneration of new tissues.

The invention relates to a preparation method of a long-term stable amphiphilic antifouling coating based on mussel bionics. The method comprises the following steps: mixing dopamine hydrochloride, sodium borate, sodium carbonate and deionized water, dropwise adding a methacrylic anhydride solution, mixing the obtained dopamine methacrylamide with mercaptopolydimethylsiloxane, polyethylene glycoldiacrylate, a photoinitiator and a solvent, coating a base material with the mixture, and curing under ultraviolet irradiation. The method has the advantages of simple process synthesis conditions, cleanness, environmental protection, rapid ultraviolet-induced cross-linking reaction and good effect; the prepared coating has good mechanical properties and stability, has a very small swelling degreein a seawater environment, has an excellent anti-algae adhesion effect and has potential application in the aspect of marine antifouling materials.

The invention relates to sericin / oxidized graphene composite hdyrogel as well as a preparation method and application thereof. The preparation method comprises the steps: blending a methacrylic anhydride modified sericin solution and oxidized graphene, then adding a photoinitiator and performing crosslinking under ultraviolet irradiation to prepare sericin / oxidized graphene composite hydrogel withgood mechanical property and osteogenic induction performance. The prepared sericin / oxidized graphene composite hydrogel has the advantages of good biocompatibility and biodegradability, high mechanical strength and osteogenic induction and application to the aspects of medicine and cell carrying, tissue engineering repairing and the like.

The invention relates to the field of medical biological dressings, in particular to a GelMA hydrogel human acellular amniotic membrane three-dimensional two-layer dressing preparation method which isa method for preparing a GelMA hydrogel / human acellular amniotic membrane three-dimensional two-layer dressing through integration of electrostatic spinning and photo-crosslinking. The method includes: subjecting gelatin to MA (methylacrylic acid) graft modification to form GelMA, covering D-HAM (decellularized human amniotic membrane) with GelMA in a porous fiber form through electrostatic spinning to obtain the three-dimensional two-layer bioactive dressing, and subjecting the dressing to photo-crosslinnking with a photoinitiator Irgacure2959; lyophilizing the dressing subjected to photo-crosslinnking, performing gamma irradiation sterilization, and packaging under sterile conditions. Great anti-inflammation and anti-infection effects and high biocompatibility are achieved, and quick healing of oral mucous wounds can be promoted.

The invention discloses a biological material, a preparation method thereof and application of the biological material as a bone defect repairing material and belongs to the technical field of medicalbiological materials. The preparation method comprises the steps of adding a photoinitiator solution into an aqueous solution containing methacrylic anhydride gelatin, wherein the photoinitiator solution is a photoinitiator 819 solution or a mixed solution of the photoinitiator 819 and a photoinitiator 184; carrying out extrusion type 3D biological printing on printing slurry, and carrying out ultraviolet irradiation on the slurry when the slurry is extruded; preferably, the aqueous solution containing methacrylic anhydride gelatin also contains carboxymethyl chitosan; preferably, chemical crosslinking is carried out after printing is finished; preferably, an inorganic bioceramic material is added to the printing slurry. The method has the advantages of simple components, stable printingprocess, high precision, high crosslinking degree, high crosslinking speed and good scaffold biocompatibility, and the biological material can also be used as a carrier of medicines or other functional components, and has very wide application prospect in the field of bone defect repair.