3D-printed degradable macromolecular scaffold and photo-crosslinked hydrogel composite scaffold
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A composite scaffold and 3D printing technology, used in prosthesis, tissue regeneration, medical science and other directions, can solve problems such as unfavorable cell adhesion and growth, and achieve the effect of good swelling effect, low swelling rate and good mechanical properties
Active Publication Date: 2020-05-19
NOVAPRINT THERAPEUTICS SUZHOU CO LTD
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However, the above literature only utilizes a single degradable polymer sca
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Embodiment 1
[0050] ①Preparation of PCL stent by 3D printing:
[0051] The printer used for 3D printing is the OPUS seven-channel bioprinting system. The molecular weight of the PCL used is 43,000. The diameter of the 3D printed fibers is 200 μm, and the distance between the fibers is 200 μm. The printing temperature is 90 ° C, 600 Psi, and 250 mm / min. speed. The specific steps of 3D printing are as follows: design the stent printing model, set the corresponding parameters, extrude through the fine nozzle, and superimpose the fiber layers to form the PCL stent.
[0052] ②Preparation of GelMA with low degree of substitution and high degree of substitution:
[0053] 1. Prepare 0.25M carbonate-bicarbonate buffer (CB buffer): add 0.3975g sodium carbonate and 0.7325g sodium bicarbonate to deionized water, then measure the pH of the solution, if the pH is less than 9.0, add HCl , if the pH is greater than 9.0 then add NaOH to bring the final pH to 9.0.
[0054] 2. Preparation reaction of GelM...
Embodiment 2
[0067] ① Preparation of PLA scaffolds by 3D printing:
[0068] The printer used for 3D printing is the OPUS seven-channel bioprinting system. The average molecular weight of the PLA used is 100,000. The diameter of the 3D printed fibers is 200 μm, and the distance between the fibers is 200 μm. The printing pressure is 100 Psi and the printing speed is 250 mm / min. . The specific steps of 3D printing are as follows: design the printing model of the bracket, set the corresponding parameters, extrude through the fine nozzle, and superimpose the fiber layers to form the PLA bracket.
[0069] ②According to the existing literature, glycidyl methacrylate (GMA) was used to modify hyaluronic acid (HA) to obtain hyaluronic acid with a low degree of substitution (30%) and a high degree of substitution (70%);
[0070] ③ The final preparation method of the composite scaffold of hyaluronic acid and PLA with different degrees of substitution and crosslinking, including the following steps:
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Abstract
The invention relates to a 3D-printed degradable macromolecular scaffold and photo-crosslinked hydrogel composite scaffold. The 3D-printed degradable macromolecular scaffold and photo-crosslinked hydrogel composite scaffold comprises a 3D-printed degradable macromolecular scaffold, wherein the interior of the 3D-printed degradable macromolecular scaffold comprises photo-crosslinked hydrogel with high substitution degree and photo-crosslinked hydrogel with low substitution degree, the photo-crosslinked hydrogel with high substitution degree is crosslinked with the photo-crosslinked hydrogel with low substitution degree, and preferably, a polycaprolactone (PCL) scaffold and methacrylic anhydride gelatin (GelMA) with different substitution degrees are crosslinked and compounded. In the composite scaffold, the 3D-printed degradable macromolecular scaffold has good mechanical properties; the photo-crosslinked hydrogel with high substitution degree has high crosslinking degree, can form a fiber network and micropores, and well supports cells; and the photo-crosslinked hydrogel with low substitution degree has multiple active sites, is beneficial to cell adhesion growth, and can adsorb alarge amount of nutrient solution. Through cooperation of the 3D-printed degradable macromolecular scaffold, the photo-crosslinked hydrogel with high substitution degree and the photo-crosslinked hydrogel with low substitution degree, the composite scaffold is suitable for cell growth and vascularization from an inner layer to an outer layer, and when the composite scaffold is used for medical human body repair, scaffold integration is realized to promote regeneration of new tissues.
Description
technical field [0001] The invention relates to the field of tissue engineering scaffolds, in particular to a composite scaffold of a 3D printed degradable polymer scaffold and photocrosslinked hydrogels with different degrees of substitution and crosslinking. Background technique [0002] Tissue engineering scaffold materials refer to materials that can be combined with living cells of tissues and implanted into organisms. The scaffold material should be beneficial to serve as a carrier of cells and promote cell reproduction and differentiation. In addition, this material needs to have a certain mechanical strength, and the scaffold is degraded into harmless metabolites. [0003] Tissue engineering scaffold materials are divided into natural scaffold materials and synthetic scaffold materials. Natural scaffold materials include collagen, gelatin, fibrin, chitosan, alginate, hyaluronic acid, etc., which are characterized by fast degradation, bioactive sites, and suitable f...
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