120 results about "Scaffold material" patented technology

The invention relates to a soft tissue incremental scaffold material and a preparation method and application thereof, and belongs to the technical field of medical materials.The preparation method of the soft tissue incremental scaffold material comprises the following steps that S1, a collagen solution and bioactive glass are evenly mixed, and a mixture is obtained; the mass ratio of collagen to the bioactive glass in the collagen solution is (4-19): 1; s2, carrying out freeze drying and crosslinking on the mixed solution to obtain a crosslinked material; s3, placing the cross-linking material in a hydrogen peroxide solution for modification treatment; the mass fraction of hydrogen peroxide in the hydrogen peroxide solution is 2-6%; and S4, repeating the step S3 until the sodium content and the crosslinking agent residue in the modified material meet the requirements, thereby obtaining the soft tissue incremental scaffold material. The soft tissue increment scaffold material prepared by the invention has proper pore structure and pH, lower cytotoxicity, higher recovery rate after compression and lower thickness increase rate after water absorption, and is beneficial to regeneration of soft tissues.

A system and method for producing an integrated optical assembly, which can include providing a functionalized scaffold material; and forming an optical assembly including at least two optical components within the scaffold material, the forming of the optical assembly including: lithographically patterning a first optical component within the scaffold material; and lithographically patterning a second optical component within the scaffold material aligned with the first optical component.

The invention relates to the technical field of biological ceramic materials, in particular to a zirconium oxide bone repair scaffold material with a porous interconnection structure and a preparation method of the zirconium oxide bone repair scaffold material. The invention aims to solve the problems of poor mechanical property and fragile interconnection structure of the existing stent under high porosity. The core preparation process comprises the following steps of: performing acetone steam annealing and pre-fusion on a double-particle-size microsphere template to form a stable interconnection window prototype; then, a continuous hole wall main body is constructed through vacuum impregnation of high-viscosity zirconium oxide slurry, and then secondary positioning reinforcement is conducted on the interconnected neck parts through low-viscosity nano zirconium oxide sol; finally, diluted bioactive glass sol is introduced to endow the surface with biological activity, and a final product is obtained through temperature-controlled degreasing and sintering. According to the method, the pore connectivity, the mechanical strength and the biological activity of the scaffold are synergistically optimized through template pre-fusion and partition sequential processes, and the method is suitable for repairing load-bearing bone defects.

The invention discloses a multi-parameter online monitoring system for aging failure of an insulating scaffold and a preparation method of the multi-parameter online monitoring system. The monitoring system comprises the insulating scaffold; the monitoring sensor is arranged on the insulating scaffold and is used for acquiring various state parameters of the insulating scaffold; the communication module is connected with the monitoring sensor and is used for transmitting the various state parameters; the on-line monitoring all-in-one machine is connected with the communication module and is used for carrying out data acquisition and comprehensive processing analysis on the signal data of the various state parameters so as to monitor the state of the insulating scaffold in real time and evaluate the aging failure risk of the insulating scaffold; the system can monitor the operation state of the scaffold in a multi-dimensional and real-time manner, and comprehensively analyzes the change trend of the parameters, so that the aging process and failure risk of the scaffold material can be more accurately judged, the abnormality can be timely found, the early warning can be performed in advance, and the occurrence of safety accidents can be prevented.

The invention relates to a mycelium biological symbiotic scaffold material as well as a preparation method and application thereof. The mycelium biological symbiotic scaffold material comprises a biological symbiotic scaffold matrix and mycelia uniformly growing on the surface of the biological symbiotic scaffold matrix, the mycelium has a three-dimensional network porous structure. The mycelium biological symbiotic scaffold material provided by the invention is a novel bone repair biological material with a multilevel structure and biological activity, and a new strategy is provided for tissue regeneration.

The present application provides a kind of block cell culture meat and its preparation method.The present application meets the demand of seed cell large-scale preparation of block cell culture meat by suspension cell scale culture, and realizes the adhesion, proliferation and integration of seed cell on edible scaffold material by specific culture medium induction of suspension cell adhering to wall.The method can be in stirred tank cell bioreactor by sterile scaffold material and cooperate with specific induction culture solution and stirring process, so that the adhesion rate of cell and scaffold material reaches 96.8%.The method uses suspension cell as seed cell, and combines corresponding process and culture medium, effectively solves the problems of seed cell difficult to be large-scale prepared and difficult to be effectively integrated with scaffold material in the culture process of block cell culture meat, and provides scalable scheme for industrialized production of block cell culture meat.

This invention provides a scaffold-free construction method for installing exterior walls of high-rise buildings, belonging to the field of scaffold-free installation technology. This invention utilizes I-beams, limiting devices, steel pipes, fall arrestors, guide devices, and fixing devices. First, the I-beams are installed on a floor platform. Limiting devices are then installed on the I-beams, providing bottom support and limiting for individual steel pipes. Moving the limiting devices causes the steel pipes to slide along the I-beams to a pre-installation position and then locks them in place, thus fixing the fall arrestor to the floor wall. This invention allows for flexible adjustment of the bottom position of the steel pipes through the sliding limiting devices. Combined with the vertical fixing and climbing structure of the guide devices and fall arrestors, the construction platform for high-rise building exterior walls can be erected layer by layer upwards without the need for full scaffolding from the ground. This significantly reduces scaffolding material consumption and the workload of high-altitude erection. The construction platform can rise synchronously with the work surface, ensuring the continuity and efficiency of exterior wall construction.

The present invention relates to a scaffold material for stem cell culture. Among them, the dispersive component γ<supgt;d< / supgt> of the surface free energy of the scaffold material for stem cell culture is 24.5 or more and less than 45.0, and the dipole component γ<supgt>p< / supgt> of its surface free energy is 1 or more and less than 20.0. According to this scaffold material for stem cell culture, it has moderate hydrophilicity and strength, and has a high fixation after inoculation of stem cells, and cell proliferation can be carried out efficiently.

To provide a resin film formed of a scaffold material for cell culture, the resin film enabling excellent fixation of cells after seeding and thus contributing to an increase in the cell proliferation rate.SOLUTION: A resin film is formed of a scaffold material for cell culture, wherein the scaffold material for cell culture comprises a synthetic resin with a polyvinyl acetal skeleton; the resin film has a phase-separation structure containing at least a first phase and a second phase; and the ratio of the surface area of one of the first and second phases to the total surface area is 0.01-0.95 inclusive.SELECTED DRAWING: Figure 1

Disclosed herein are compositions useful as artificial dermal layer scaffold materials. Methods of making and processing scaffold materials for artificial dermal layers are disclosed herein. Disclosed herein are scaffold materials for supporting immortalized fibroblasts.

To provide a scaffolding material for cell culture that has excellent transparency and can be used in scaffold-type cell culture, particularly a scaffolding material for cell culture that can form a scaffolding material with a hydrogel pattern using a simple method by exposure and development.SOLUTION: Provided is a scaffolding material for cell culture containing a fibrous cellulose with an average fiber width of 100 nm or less, and a negative radiation-sensitive component containing a polymerizable compound and a polymerization initiator, the content of the negative radiation-sensitive component being 20 to 200 pts.mass based on 100 pts.mass of the fibrous cellulose, the negative radiation-sensitive component being a component that is soluble in at least one selected from water, alcohol, and a mixed solvent thereof.SELECTED DRAWING: None

The utility model discloses a general supporting structure for a post-cast strip. The general supporting structure comprises a lifting single prop, lifting supporting rods are arranged in the steel pipes in a sliding and penetrating mode, sleeves arranged on the corresponding lifting supporting rods and the telescopic ends of the lifting single supporting columns in a sleeving mode are fixed to the supporting top plate, locking screws are connected to the sleeves in a penetrating and threaded mode, and the ends of the locking screws abut against the corresponding lifting supporting rods and the telescopic ends of the lifting single supporting columns. The lifting single supporting columns and the steel pipes are connected through connecting frames. The supporting structure has the advantages that during pouring construction of the post-cast strip, a scaffold is built in a construction area, and the supporting structures are erected on the two sides of the post-cast strip at intervals; when post-cast strip construction is completed and concrete design strength is achieved, the scaffold can be dismantled, the supporting structure serves as an independent support to continue to support the two sides of the post-cast strip within the driving route range, and therefore the dismantled scaffold material can be repeatedly used for other construction areas, turnover and reutilization of the material are achieved, and the construction efficiency is improved. The construction cost is reduced.

The invention relates to a gradient scaffold material based on particle gel as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) constructing a shaping framework; (2) respectively preparing a plurality of hydrogel particles with different properties; and (3) respectively injecting the hydrogel particles with different properties into the shaping frame, and carrying out in-situ crosslinking on the hydrogel particles to prepare the gradient scaffold material. The scaffold material prepared by the method has the characteristic that the composition, the structure and the mechanical property are continuously adjustable in space, and the scaffold also has good biocompatibility and a penetrating porous structure, so that inward migration, proliferation and directional differentiation of cells are facilitated, and the scaffold material has good application prospects. The method has important application in the fields of bone-cartilage, tendon-bone, skin and other gradual change tissue engineering interface repair and the like.

The invention relates to a 3D-printed bone repair scaffold material with double functions and a preparation method and application thereof, polycaprolactone, nano barium titanate and citrate-modified amorphous calcium phosphate are uniformly mixed, heated for reaction and ultrasonically dispersed to prepare biological printing ink, and the bone repair scaffold material is obtained through 3D printing. The bone repair scaffold material prepared by the invention has shape memory and piezoelectricity, is good in biocompatibility, and can promote osteogenic differentiation and bone defect repair.

The application belongs to the technical field of bone tissue engineering, and particularly relates to a recombinant collagen-chitosan-PEG-hydroxyapatite bone repair scaffold material, a preparation method and application. The bone repair scaffold material is formed into a one-way ordered three-dimensional scaffold by a directional freezing method through recombinant collagen, chitosan and PEG. Nano-hydroxyapatite is uniformly deposited in the inside and surface through a special mineralization process. The prepared material has a very good bionic natural bone structure and composition, a good reticular structure and mechanical strength, good biocompatibility and cell activity, a significant repair effect on critical size bone defects, a good osteogenic induction effect and bone conductivity. The bone repair scaffold material provides a new potential treatment method for severe bone defects, and has a wide application in the fields of artificial bone, artificial cartilage, biological scaffolds and the like.

The disclosure relates to a scaffold graft material, or platelet rich plasma (PRP) graft, and method of production thereof, for tissue repair and / or support (e.g. reinforcement). In one particular application, the scaffold material is used for treating pelvic organ prolapse (POP).

The invention belongs to the technical field of biological materials, and particularly relates to an auricular cartilage scaffold material as well as a preparation method and application thereof. According to the preparation method of the auricular cartilage scaffold material, thermoplastic polyurethane is selected as a base material, sodium chloride is taken as a pore-forming agent, and the auricular scaffold material matched with the mechanical property of the auricular cartilage of a normal person is successfully constructed by regulating and controlling material processing parameters. Experiments prove that the scaffold material prepared by the invention not only has structural stability and biocompatibility, but also has mechanical properties matched with those of natural auricular cartilage. Therefore, a new theoretical basis and a technical strategy can be provided for personalized treatment of auricle reconstruction, and a solid foundation can be laid for clinical transformation and application of a novel auricle support.

The invention relates to an extracellular vesicle-frozen gel scaffold as well as an integrated preparation method and application thereof, and belongs to the technical field of biotechnology and tissue engineering materials. The problems that in the prior art, extracellular vesicles are prone to being damaged by low temperature in the frozen gel preparation process, a traditional cryoprotectant is not compatible with a stent process, and burst release and action time is short in a vesicle physical adsorption method are solved. The method comprises the following steps: mixing extracellular vesicles with a trehalose solution, and standing to obtain a vesicle-trehalose mixed solution; mixing with the support material prepolymer solution, and adding a photoinitiator to form a composite precursor solution; freezing the solution, and performing cross-linking solidification to form a three-dimensional porous gel scaffold; and unfreezing and washing to obtain the stent. The trehalose is introduced as a cryoprotectant, vesicle activity protection and stent structure forming are synchronously achieved in low-temperature forming, and the prepared stent can achieve controllable slow release of vesicles and has good application prospects in the field of tissue repair and regeneration.

The invention relates to a decellularized scaffold composite dynamic hydrogel material which comprises an interpenetrating network structure formed by a decellularized scaffold and dynamic cross-linked hydrogel, and the dynamic cross-linked hydrogel is formed by cross-linking initiated by hydrophobic group modified hyaluronic acid and cross-linked group modified cyclodextrin. The dynamic crosslinking hydrogel further comprises hydrogel formed by crosslinking initiated by gelatin or crosslinking group modified glycosaminoglycan. The invention also relates to a preparation method of the composite material. The preparation method comprises the following steps: preparing a pre-gel solution from the hydrophobic group modified hyaluronic acid, the crosslinking group modified cyclodextrin and the crosslinking initiator; and dripping the pre-gel solution on the surface of the decellularized scaffold, and initiating cross-linking to obtain the hydrogel-decellularized scaffold composite material. The decellularized scaffold composite dynamic hydrogel material satisfies three characteristics of mechanical properties, biological adaptation degradability and structural dynamic heterogeneity, is a scaffold material better imitating an extracellular matrix, and has an application prospect in the field of tissue repair.

The utility model belongs to the field of trolley, especially a bridge shed integrated inner and outer mould trolley, aim at the low efficiency of the existing bridge shed construction: template needs repeatedly dismounting, transport, the erection and removal process of full hall scaffold can easily cause safety accidents; Scaffold material and labor cost are high, the problem that field assembly precision is difficult to control, present the following scheme, it includes inner mould trolley and outer mould trolley, the inner mould trolley includes inner frame stand, the lateral work platform is fixedly installed to the side of inner frame stand, a plurality of inner frame stand are fixedly connected through longitudinal scissors and longitudinal connecting beam between the side, in the utility model, inner mould trolley and outer mould trolley work cooperatively, realize the full process automation of bridge shed construction, through the mechanism such as vertical cylinder in stand, side mould upper translation cylinder, realize the quick stripping and translation of template, common rail walking mechanism: inner mould trolley and outer mould trolley common rail design, outer mould trolley can demould in advance and walk independently, avoid construction conflict.

This cell culture kit (100) is provided with a culture vessel (110) and a culture sheet (150). The culture sheet (150) is a scaffold material for culturing cells. The culture container (110) has therein an accommodation space, in which the culture sheet (150) is accommodated, and includes an opening that communicates with the accommodation space. The culture container (110) includes a bottom part (112) on which the culture sheet is placed, and a side wall part (113) extending upward from the bottom part (112). The culture sheet (150) has a shape and size such that when placed on the bottom part (112) a gap is formed between the outer edge part of the culture sheet (150) and the side wall part (113).

This invention provides a method for constructing ultra-high-rise buildings with ultra-long span simply supported floor slabs without support, comprising: hoisting and connecting structural steel columns; hoisting structural steel beams and connecting them to the corbels of the structural steel columns; laying floor slabs; welding studs to the top of the structural steel beams to fix the galvanized bottom formwork of the floor slabs; installing lower reinforcing bars; installing additional lower reinforcing bars on the floor slabs at the breaks or perpendicular intersections with the structural steel beams; installing upper reinforcing bars; installing additional upper reinforcing bars on the floor slabs at the breaks or perpendicular intersections with the structural steel beams; and pouring concrete. This invention eliminates the need for traditional full-span scaffolding support, enabling the construction of reinforced concrete for simply supported floor slabs exceeding the maximum unsupported span at high altitudes. It shortens the construction period, saves on scaffolding material usage and erection costs, avoids instability in ultra-high support scaffolding, ensures safety during high-altitude operations, fully utilizes the original design's upper and lower chord reinforcing bars and additional upper and lower reinforcing bars, requires no additional construction materials, and is environmentally friendly.

This invention discloses a method for preparing and applying a 3D-printed strong and tough silk fibroin scaffold material. The method includes: incubating silk fibroin fibers in an aqueous lithium bromide solution; modifying with glycidyl methacrylate followed by freeze-drying; obtaining a bio-ink by dissolving in an aqueous photoinitiator solution; obtaining an intermediate solution by dissolving a calcium salt in a methacryloyl phosphate compound; obtaining a photocurable bio-ink by dissolving the intermediate solution in the bio-ink; and photocuring and printing in a 3D printer to obtain the 3D-printed strong and tough silk fibroin scaffold material. This method offers advantages such as good formability, flowability compatible with 3D printers, suitability for various 3D printing modes, and rapid photocuring performance. The prepared scaffold exhibits good biocompatibility, and the printed gel material shows better mechanical properties and stability compared to SilMA, with a slower degradation rate, enabling the scaffold to be widely used in multiple fields such as bone tissue engineering.

The present disclosure is directed to scaffold materials patterned with cell adhesion targets that permit the attachment and growth of predetermined cell types onto the scaffold. By selecting specific ligands that are patterned on the scaffold, particular cell types, including those engineered for this specific purpose, will attach and proliferate to create designed tissues and organs.

This invention relates to the field of bioceramic materials technology, specifically to a porous interconnected zirconia bone repair scaffold material and its preparation method. This invention aims to address the problems of poor mechanical properties and fragile interconnected structures in existing scaffolds under high porosity. The core preparation process includes: using a dual-size microsphere template, pre-fusion via acetone vapor annealing to form a stable interconnected window prototype; subsequently, constructing a continuous pore wall body by vacuum impregnation with high-viscosity zirconia slurry; then, using low-viscosity nano-zirconia sol for secondary positioning and reinforcement of the interconnected necks; finally, introducing diluted bioactive glass sol to impart surface bioactivity, followed by temperature-controlled degreasing and sintering to obtain the final product. This method, through template pre-fusion and a zoned sequential process, synergistically optimizes the scaffold's pore connectivity, mechanical strength, and bioactivity, making it suitable for the repair of weight-bearing bone defects.

To provide a novel method for producing induced pluripotent stem cells (iPS cells) employing a novel scaffold material that can reduce problems associated with conventional microcarriers, and a method for producing differentiated cells using the iPS cells obtained by the production method.SOLUTION: Somatic cells are brought into contact with reprogramming factors, and induced pluripotent stem cells are established in the presence of a sheet-like piece having a surface containing a polymer material as at least one principal surface.SELECTED DRAWING: Figure 1

The invention discloses a degradable macroporous composite tissue engineering scaffold, a structural performance regulation and control method and application, and belongs to the technical field of biomedical materials. Induced phase separation is realized through design of gel molecules and a solvent, so that a macroporous structure is formed; the pore structure is adjusted by controlling the proportion of the polyvinyl alcohol aqueous solution, the glycerol and the hydrophilic degradable biomacromolecule aqueous solution; the mechanical property of the stent is regulated and controlled by adding the degradable inorganic micro-nano particles; meanwhile, a pore structure and a scaffold material with spatial heterogeneity in mechanical property are constructed through hot melt interface integration. The problems that an existing tissue engineering scaffold is insufficient in controllability of a pore structure and mechanical properties and the like are solved, and the degradable macroporous composite tissue engineering scaffold material is suitable for being used as a degradable macroporous composite tissue engineering scaffold material for promoting collaborative injury repair of soft and hard tissues such as osteochondral tissues, muscular bones and tendon bones.

Methods for fabricating three-dimensional (3D) constructs from a mixture of cells, culture medium, and a scaffold material with a controlled calcium chloride concentration is disclosed. The process involves positioning a nozzle within a gel composed of alginate solution. The nozzle is then moved within the gel, releasing the cell-media-scaffold mixture in a controlled pattern. As the mixture is deposited, it becomes surrounded by the gel, which provides structural support until it solidifies through the cross-linking reaction between the calcium chloride in the cell-media-scaffold mixture and the alginate solution. This results in the formation of a solid 3D structure that incorporates the cells and their supporting scaffold. The presented method offers a versatile platform for generating intricate cell-based 3D constructs with precise control over their composition and structure. This method can be used to produce cultivated meat with complex 3D structures as well as organelles and fully functional organs.

This invention relates to the field of biomedical materials technology, providing a heparinized dual-growth factor recellularized adipose tissue decellularized scaffold material, its preparation method, and its applications. The preparation method includes: decellularizing adipose tissue to obtain adipose tissue decellularized scaffold; heparinizing the scaffold to obtain a heparinized adipose tissue decellularized scaffold; loading hepatocyte growth factor and vascular endothelial cell growth factor onto the scaffold to obtain a heparinized dual-growth factor adipose tissue decellularized scaffold; seeding induced pluripotent stem cell-derived hepatocytes onto the scaffold, and culturing to obtain a heparinized dual-growth factor recellularized adipose tissue decellularized scaffold (RAT). This invention uses rat inguinal adipose tissue to prepare RAT material, which is low-cost, readily available, has a simple preparation process, and is easy to scale up. The RAT material of this invention can achieve liver tissue damage repair and hepatocyte regeneration, providing a new treatment strategy for acute liver failure.