Construction method of anticoagulant artificial blood vessel scaffold material

A stent material and artificial blood vessel technology, which is applied in the construction field of anticoagulant artificial vascular stent materials, can solve the problems affecting the persistence of NO release, the fast release rate of NO, and the phenomenon of burst release, so as to improve the phenomenon of catalytic NO burst release , overall performance optimization, the effect of inducing and promoting angiogenesis

Inactive Publication Date: 2013-03-13
NANKAI UNIV
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Problems solved by technology

Although it can play the purpose of catalyzing the release of NO, because this type of evenly mixed and loaded in vitro catalyst loading on the polymer microfiber is unstable, especially hydrophilic substances are easy to fall off from the surface of the polymer microfiber membrane, thereby make the local Cu 2+ If the concentration is to

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  • Construction method of anticoagulant artificial blood vessel scaffold material
  • Construction method of anticoagulant artificial blood vessel scaffold material

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1. The construction method of anticoagulant artificial vascular stent material, comprises the following steps successively:

[0025] ① Preparation of P3HB4HB electrospinning solution with P3HB4HB and Cu 2+ Mixed electrospinning solution of -Cyclen complex:

[0026] Weigh 300mg of 12% P3HB4HB, dissolve in 6ml solvent (dichloromethane:methanol=9:1); prepare three parts; one part is dissolved in 1.2mg Cu 2+ -Cyclen complexes;

[0027] ② Preparation of porous vascular stent material with three-layer structure by electrospinning technology:

[0028] Using a stainless steel tube with an inner diameter of 3 mm as the electrospinning receiver, the above-mentioned P3HB4HB electrospinning liquid, P3HB4HB and Cu 2+ -Cyclen complex mixed electrospinning solution, and P3HB4HB electrospinning solution electrospun on the tubular receiver to form figure 1 The artificial vascular stent shown;

[0029] Electrospinning conditions: the temperature is 25°C, the air humidity ...

Embodiment 2

[0031] Embodiment 2. The construction method of anticoagulant artificial vascular stent material, comprises the following steps successively:

[0032] ①Preparation of PCL electrospinning solution with PCL and Cu 2+ - Mixed electrospinning solution of Cyclam complex:

[0033] Weigh PCL, 300mg, dissolve in 6ml solvent (dichloromethane:methanol=9:1); prepare three parts; one part is dissolved in 1.2mg Cu 2+ - Cyclam complexes;

[0034] ② Preparation of porous vascular stent material with three-layer structure by electrospinning technology:

[0035]Using a stainless steel tube with an inner diameter of 3mm as the electrospinning receiver, the above-mentioned PCL electrospinning solution, PCL and Cu 2+ -Cyclam complex mixed electrospinning solution, and PCL electrospinning solution electrospun on the tubular receiver to form figure 1 The artificial vascular stent shown;

[0036] Electrospinning conditions: the temperature is 25°C, the air humidity is 40%, the flow rate is 1ml / ...

Embodiment 3

[0038] Embodiment 3. The construction method of anticoagulant artificial vascular stent material, comprises the following steps successively:

[0039] ① Preparation of PCL and PLGA mixed electrospinning solution, PCL and PLGA and Cu 2+ - Mixed electrospinning solution of DTTCT complex: Weigh 150 mg each of PCL and PLGA, dissolve in 6 ml solvent (dichloromethane: methanol = 9: 1); prepare three parts; one part is dissolved in 1.2 mg Cu 2+ - DTTCT complex;

[0040] ② Preparation of porous vascular stent material with three-layer structure by electrospinning technology:

[0041] Using a stainless steel tube with an inner diameter of 3 mm as the electrospinning receiver, the above-mentioned PCL and PLGA electrospinning solution, PCL and PLGA and Cu 2+ -Cyclam complex mixed electrospinning solution, and PCL and PLGA electrospinning solution are electrospun on the tubular receiver to form figure 1 The artificial vascular stent shown;

[0042] Electrospinning conditions: the temp...

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Abstract

The invention discloses a construction method of an anticoagulant artificial blood vessel scaffold material. The method includes: taking a rotating stainless steel pipe as a receiver, employing an electrospinning technology to prepare an organic macromolecular polymer, a mixture of a Cu2<+> complex catalyst and the organic macromolecular polymer, and the organic macromolecular polymer successively into a three-layer structured superfine fiber porous tubular scaffold material; and regulating the types and proportion of the Cu2<+> complex catalyst and the organic macromolecular polymer, as well as the electrospinning time of a three-layer electrospinning solution so as to control the release rate of NO. With a three-layer structure, the blood vessel scaffold material formed by the invention not only realizes loading of the Cu2<+> organic complex catalyst, but also improves the phenomenon of NO burst release catalyzed by an ordinary hybrid electrospun structure scaffold material, improves the loading stability of the Cu2<+> complex catalyst, and can leave the biological signal molecule NO to play a better role in inhibiting platelet adhesion, resisting platelet activation and inhibiting smooth muscle cell proliferation so as to improve its anticoagulant property.

Description

Technical field: [0001] The invention relates to a method for modifying the surface of a tissue engineering vascular stent material, in particular to a method for constructing an anticoagulant artificial vascular stent material. Background technique: [0002] There is a great demand for small-caliber blood vessels clinically, but the current small-caliber artificial blood vessels have a high rate of restenosis after implantation in the body. In order to clinically reduce the harm caused by thrombosis, anticoagulants are often used in medicine. However, long-term use of exogenous anticoagulants also has side effects, especially increasing the possibility of bleeding. [0003] Endothelial cells are natural regulators of blood vessel stability. Endothelial cells play an important role in antithrombosis, inhibition of platelet aggregation, and secretion of vasoactive factors. Molecules with antithrombotic, antiplatelet aggregation properties in endothelial cells include nitric...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/56A61L27/50A61L27/54A61F2/07
Inventor 王淑芳邓秋萍安军周维刘洋陈列阳
Owner NANKAI UNIV
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