47 results about "Platelet adhesion" patented technology

The invention relates to the technical field of coating surface preparation, in particular to an anticoagulant coating based on novel tick-derived anticoagulant protein Qinghaenin and a preparation method of the anticoagulant coating. The functional layer of the anticoagulant coating comprises the following components: anticoagulant protein Qinghaenin or a functional peptide fragment Qinghaenin 167-192 of the anticoagulant protein Qinghaenin, wherein the amino acid sequence of the functional peptide fragment is as shown in SEQ ID NO. 2. The preparation method of the anticoagulant coating comprises the following steps: by using a polydopamine coating as a substrate layer, covalently grafting through an amido bond, immobilizing anticoagulant protein Qinghaenin capable of inhibiting FXII factor activation or a functional peptide fragment Qinghaenin 167-192 thereof on the surface of a medical polymer material, and successfully constructing a novel anticoagulant coating. Compared with an unmodified medical polymer material, the modified coating can significantly enhance the hydrophilic performance of the material and inhibit platelet adhesion and activation, finally effectively prolongs the in-vitro blood coagulation time, and shows excellent anticoagulation performance.

The invention relates to the technical field of medical artificial intelligence, in particular to a vascular embolism risk prediction method based on big data analysis, and the method comprises the steps: collecting a pulse pressure fluctuation rate, a microcirculation resistance index and a platelet adhesion gradient through a multi-source sensor, and extracting dynamic physiological parameter time sequence features; constructing a hemodynamic finite element model based on CTA and VFI image data, and obtaining a vascular mechanical feature vector; deep coupling of the pulse pressure spectrum and the circumferential stress is achieved through dynamic time warping and convolution mapping, and a multi-modal risk feature set is generated; and performing cross-modal coding by using the double-branch graph neural network, and outputting an acute embolism probability and a three-dimensional risk thermodynamic diagram. According to the method, physiological, image and mechanical multi-dimensional information is fused, and the method has the advantages of being high in real-time performance, accurate in positioning and good in interpretability and is suitable for risk prediction and auxiliary diagnosis of clinical cardiovascular and cerebrovascular events.

The invention relates to the technical field of blood purification materials, in particular to a zirconium-titanium bimetal MOF hollow microsphere adsorbent and a preparation method and application thereof. The adsorbent is based on a Zr / Ti bimetallic node and a phosphonic acid-carboxylic acid mixed ligand, a composite MOF hollow microsphere structure with high stability and high specific surface area is constructed through a solvothermal and polymerization synergistic reaction in a water-in-oil suspension system, a phosphonic acid group and Zr are strongly coordinated to improve the stability of a framework, and the composite MOF hollow microsphere structure has a high specific surface area. The carboxylic acid group keeps the hydrophilicity of the pore channel; meanwhile, a cross-linked network of acrylamide and N, N '-methylene bisacrylamide penetrates through crystal gaps in the balling process, so that the material has excellent mechanical strength and fluid stability; and a sulphobetaine amphoteric polymer brush layer is constructed on the surface of the MOF through endogenous initiation point modification and surface in-situ controllable free radical polymerization technologies, so that protein adsorption and platelet adhesion are effectively inhibited, the blood compatibility is remarkably improved, and meanwhile, the channel accessibility is maintained to promote rapid diffusion and adsorption of bilirubin.

The invention belongs to the technical field of medicines, and discloses application of pantethine in preparation of medicines for preventing and / or treating thrombotic diseases or medicines for inhibiting platelet activation or regulating vascular endothelial cell functions. In-vivo and in-vitro experiments prove that pantethine can remarkably relieve thrombus load in a mouse thrombus model, down-regulate expression of vWF (von Willebrand Factor), up-regulate expression of TFPI (Tissue Factor Path Inhibitor) and TH BD (Thromboregulatory Protein), and antagonize platelet adhesion, aggregation and activation induced by a platelet activator. The action mechanism is closely related to inhibition of reactive oxygen species (ROS) generation and NF-kappa B signal channel activation, and improvement of endothelial cell functions. It can be known from the invention that pantethine plays an antithrombotic role from multiple aspects, and is suitable for preparation of antithrombotic drugs for prevention or treatment. The invention provides a new drug choice for prevention and treatment of thrombotic diseases.

The application relates to the field of blood purification materials, and particularly discloses an anion exchange resin membrane applied to blood purification and a preparation method thereof. The anion exchange resin membrane applied to blood purification is prepared by blending a zwitterion-anion exchange copolymer and a hydrophobic film-forming polymer; the zwitterion-anion exchange copolymer is copolymerized by an anion exchange monomer and a zwitterion monomer, so that the resin membrane has anion exchange function and anti-protein adsorption function; the preparation method is as follows: the anion exchange monomer and the zwitterion monomer are subjected to free radical copolymerization under the action of an initiator; the copolymer and polyether sulfone are dissolved in a polar aprotic solvent to prepare a casting solution, and after casting film formation, phase inversion is completed by immersing in a coagulation bath; the obtained membrane is cleaned and subjected to alkalization treatment. The application solves the problems that traditional anion exchange resins easily cause non-specific protein adsorption, platelet adhesion and complement system activation.

The application relates to the technical field of coating surface preparation, in particular to an anticoagulant coating based on a novel tick-derived anticoagulant protein Qinghaienin and a preparation method thereof. 167‑192 Wherein the amino acid sequence of the functional peptide segment is shown in SEQ ID NO. 2. The anticoagulant coating is prepared by using a polydopamine coating as a base layer, and then covalently grafting the anticoagulant protein Qinghaienin or the functional peptide segment Qinghaienin 167‑192 which can inhibit the activation of the FXII factor is covalently grafted through an amide bond to the surface of a medical polymer material, and a novel anticoagulant coating is successfully constructed. Compared with unmodified medical polymer materials, the modified coating can significantly enhance the hydrophilicity of the material, inhibit platelet adhesion and activation, effectively prolong the blood clotting time in vitro, and exhibit excellent anticoagulant performance.

The application provides a platelet-responsive self-gelation hemostatic material and a preparation method and application thereof, and belongs to the medical field. Based on a multiple cross-linking strategy of dynamic covalent bonds and non-covalent interactions, a hemostatic material capable of realizing self-gelation quickly is designed by using modified sodium alginate and hydroxylated porcine small intestinal submucosa as raw materials. The hemostatic material has good hydrophilicity and liquid absorption capacity, can realize powder-gelation transformation in a short time, form a stable physical barrier, has platelet response characteristics, can promote platelet adhesion and activation, accelerate the blood coagulation process of the body, and improve the mechanical properties and tissue adhesion of the material, and has a good application prospect.

The invention discloses a polyurethane surface bionic coating capable of synergistically resisting blood coagulation and promoting endothelialization and a preparation method, and relates to the technical field of biomedical materials. According to the invention, tannic acid (TA) and polylysine (PLL) are subjected to a Schiff base and Michael addition reaction in a weak alkaline environment to form a covalent cross-linked bionic intermediate layer, and the intermediate layer synergistically loads heparin (Hep) and laminin (Ln) on the surface of polyurethane (PU) to form a multifunctional active coating, so that the polyurethane material with the bionic coating is formed. The surface coating of the polyurethane material significantly reduces platelet adhesion and hemolysis rate, promotes endothelial cell adhesion, proliferation and functional expression, and inhibits excessive proliferation of smooth muscle cells, and the polyurethane material is simple in preparation process, mild in condition and suitable for surface functional modification of blood contact materials such as artificial blood vessels and interventional catheters.

The present application discloses a SERS probe combination and kit and a method for identifying and / or quantifying platelet-adherent CTC. The probe combination comprises: a first probe comprising a nanoparticle, a Raman reporter molecule and a first recognition element comprising an agent that specifically recognizes a circulating tumor cell (CTC); and a second probe comprising a nanoparticle, a Raman reporter molecule and a second recognition element comprising an agent that specifically recognizes a platelet.

The invention discloses a preparation method of an expanded polytetrafluoroethylene (ePTFE) small-caliber artificial blood vessel based on directly grafted heparin of polydopamine nanoparticles. The method comprises the following steps: performing plasma treatment on a cleaned ePTFE small-caliber artificial blood vessel, grafting PDA-NPs on the treated artificial blood vessel, activating heparin by using EDCHCl and NHS, and reacting the ePTFE / PDA / HEP small-caliber artificial blood vessel grafted with the PDA-NPs with an activated heparin solution to obtain the heparin-grafted ePTFE / PDA / HEP small-caliber artificial blood vessel. And finally, washing and freeze-drying to obtain the artificial blood vessel. By utilizing the synergistic effect of plasma treatment and PDA-NPs modification, the loading capacity and stability of heparin are remarkably improved, the prepared artificial blood vessel has excellent anti-platelet adhesion performance, extremely low hemolysis rate and good biocompatibility, no toxic coupling agent needs to be added in the whole process, the safety is high, the process is stable, and the preparation method is suitable for industrial production. The method has a wide application prospect in the field of clinical transformation of small-caliber artificial blood vessels.

The invention relates to the technical field of medical polymer materials, and particularly discloses a nylon material for a medical catheter as well as a preparation method and application of the nylon material. The nylon material for the medical catheter is mainly prepared from the following raw materials in percentage by weight: 80-90% of nylon resin, 8-12% of organic grafted SEBS (Styrene-Ethylene-Butylene-Styrene) and 2-8% of an anticoagulant, the nylon resin is selected from nylon 12 and nylon 6, and the weight ratio of the nylon 12 to the nylon 6 is (6-8): (2-4); the organic grafted SEBS is obtained by treating SEBS resin by adopting maleic anhydride, 2-acrylamido-2-methylpropanesulfonic acid and hydroxyethyl methylacrylate. Through mutual cooperation of the raw materials, the nylon material has the advantages of high tensile strength, high elongation at break, good anticoagulation performance, good platelet adhesion resistance and high biological safety, achieves balance in mechanical properties, blood compatibility and biological safety, and meets the requirements of medical catheters.

The invention provides a platelet response type self-gelation hemostatic material as well as a preparation method and application thereof, and belongs to the field of medicines. Based on multiple cross-linking strategies such as dynamic covalent bonds and non-covalent interaction, modified sodium alginate and hydroxylated pig small intestine submucosa are used as raw materials, and the hemostatic material capable of rapidly achieving self-gelation is designed. The hemostatic material has good hydrophilicity and liquid absorption capacity, can realize powder-gelation conversion in a short time to form a stable physical barrier, has platelet response characteristics, can promote platelet adhesion, activate and accelerate the coagulation process of an organism, and improves the mechanical properties and tissue adhesion of the material, so that the hemostatic material has good application prospects. Good application prospects are realized.

This invention discloses a method for surface-modified biomaterials and a modified pericardial material, relating to the field of biomedical materials technology. The method includes the following steps: S1, providing animal pericardial material cross-linked with glutaraldehyde; providing a bridging molecule containing a first bridging group and a second bridging group, and a phosphorylcholine polymer with a third bridging group at its end; binding the first bridging group to an aldehyde group on the surface of the pericardial material via a first covalent reaction; binding the second bridging group to the third bridging group via a second covalent reaction, thereby achieving grafting of the phosphorylcholine polymer onto the material surface; the first covalent reaction and / or the second covalent reaction include Schiff base formation and reductive amination processes. The modified pericardial material prepared in this application introduces a phosphorylcholine biomimetic coating, which can improve blood compatibility on the material surface, reduce activation of the coagulation system and platelet adhesion, and inhibit the initiation of calcium ion deposition on the material surface, while exhibiting high safety.

The invention discloses a hemostatic sponge preparation method, which comprises: S1, dissolving methacrylic anhydride modified gelatin in deionized water to obtain a methacrylic acid esterified gelatin solution; s2, dissolving methacrylic anhydride modified methyl cellulose in deionized water to obtain a methacrylic acid esterified methyl cellulose solution; s3, mixing the metacrylic acid esterified gelatin solution and the metacrylic acid esterified methyl cellulose solution, and then adding a photoinitiator for photo-crosslinking to construct hydrogel with a multi-network structure; s4, the hydrogel is placed in a freeze-drying machine for freeze-drying treatment, and the hemostatic sponge of a porous structure is formed; the invention also discloses the hemostatic sponge prepared by the preparation method of the hemostatic sponge, the hemostatic sponge prepared by the preparation method of the hemostatic sponge provided by the invention has good degradability, and hydrogel with a multi-network structure is formed by introducing a double-bond chemical group and utilizing photo-crosslinking. The mechanical property and the water absorption performance of the material are remarkably improved, and platelet adhesion and hemostasis promotion are facilitated.

This invention provides a method for preparing a deep vein catheter and its inner and outer walls with zwitterionic hydrogel coatings. The catheter of this invention includes: a catheter substrate; an adhesive coating covering the inner and outer walls of the catheter; an inner wall coating and an outer wall coating (4); wherein the inner wall coating and the outer wall coating are respectively fixed to the inner and outer surfaces of the catheter substrate by the adhesive coating; and both are zwitterionic hydrogel coatings. By simultaneously constructing this hydration barrier on the inner and outer walls of the catheter, this invention can block platelet adhesion and coagulation pathway activation from the initial stage of thrombosis; at the same time, the semi-interpenetrating network or microgel-reinforced structure significantly improves the mechanical stability and durability of the hydrogel, making it suitable for long-term indwelling catheters.

The invention relates to a filtration unit for enabling the selective leucocytapheresis of a fluid containing blood platelets. The filtration unit includes an external pouch provided with an inlet orifice and an outlet orifice, the pouch enclosing a porous element interposed between the orifices. The porous element encloses a leucocytapheresis medium impregnated with a polymer, the polymer having the general formula -(A)m-(B)n- (I), wherein A is a unit originating from an alkoxyalkyl acrylate or alkoxyalkyl methacrylate monomer, B is a unit originating from a hydrophobic polymerisable monomer, and m and n are integers, the sum of which, m+n, is equal to 100, m being an integer greater than 80.

The invention discloses a multifunctional blood compatibility coating for a medical apparatus and a preparation method and application thereof. The coating is formed by copolymerizing a functional monomer containing boron and sulfur elements and a bionic monomer containing phosphorylcholine. Wherein the boron element ensures tough adhesion of the coating on base materials such as plastic and stainless steel, and cooperates with sulfur to realize long-acting anticoagulation; as an active oxygen response unit, the sulfur element endows the coating with intelligent anti-inflammatory and anti-oxidation functions; and the phosphorylcholine structure on the outer layer can effectively resist protein and platelet adhesion. According to the invention, anticoagulation, anti-inflammation, anti-oxidation and strong adhesion functions are integrated, so that the limitation of single function and short-term effectiveness of the existing coating is overcome. The coating is suitable for blood contact instruments such as in-vivo blood sampling devices and intravascular stents, and thrombus and inflammation risks can be remarkably reduced.

The invention discloses a method for surface modification of a biological material and a modified pericardium material, and relates to the technical field of biomedical materials. The method comprises the following steps: S1, providing an animal pericardium material subjected to glutaraldehyde crosslinking treatment; providing a bridging molecule comprising a first bridging group and a second bridging group, and a phosphorylcholine polymer terminal-modified with a third bridging group; combining the first bridging group with the aldehyde group on the surface of the pericardium material through a first covalent reaction; combining the second bridging group with the third bridging group through a second covalent reaction to realize grafting of the phosphorylcholine polymer on the surface of the material; the first covalent reaction and / or the second covalent reaction comprise Schiff base formation and reductive amination processes. According to the prepared modified pericardium material, the phosphorylcholine bionic coating is introduced, the surface blood compatibility of the material can be improved, blood coagulation system activation and platelet adhesion can be reduced, deposition starting of calcium ions on the surface of the material can be inhibited, and safety is high.

The invention belongs to the field of dense net stents, and particularly relates to a dense net stent with a phosphorylcholine coating, the dense net stent comprises a dense net section formed by weaving a plurality of metal wires, positioning sections protruding outwards are formed at the two ends of the dense net section, and ray marking points are arranged on the positioning sections; the phosphorylcholine coating is formed on the end face, attached to the blood vessel wall, of the dense net section, surface modification is conducted on the metal wire, and the phosphorylcholine coating is used for simulating a human cell membrane structure, remarkably reducing the risk of platelet adhesion and thrombosis, improving biocompatibility, reducing the vascular intimal inflammatory reaction, supporting faster endothelial healing and shortening the recovery cycle of a patient. And the occurrence of postoperative complications is reduced. According to the method, plasma is combined to activate the metal surface, the wettability of the coating is improved, it is ensured that the intersections of the net wires are evenly covered, and in the chemical grafting method process, the dense net section rotates at a constant speed with the assistance of a negative pressure adsorption device, and coating solution accumulation or coating missing is avoided.

A container, e.g., a bag, for processing whole blood or a whole blood component and a system for processing whole blood or a whole blood component including the container. The container includes a body and an interior chamber. The body has a bulk portion and a surface portion defining an interior surface of the body. The bulk portion of the body is formed from a polymeric composition comprising a thermoplastic polymer. The surface portion of the body includes a biocompatible material that reduces platelet activation, platelet aggregation, or adhesion of platelets to the interior surface of the body. The interior chamber is at least partially defined by the interior surface of the body.

A titanium superhydrophobic surface comprising a coating of a perfluorosilane on contiguous titania micrograins surrounded by crevices, the micrograins comprising a topography of nanopits shaped by contiguous peaks and valleys. The superhydrophobic surface has significantly increased hemocompatibility and enhanced antibacterial properties compared to an unmodified titanium surface. The increased hemocompatibility was confirmed by reduced platelet adhesion, reduced leukocyte adhesion, and reduced thrombus formation. The surface is prepared by a facile hydrothermal treatment of a titanium surface with sulfuric acid, leading to the formation of a unique micro-nano surface topography. The superhydrophobic surface was then achieved by coating the micro-nano titanium surface with a low energy silane using physical vapor deposition.

The invention relates to the technical field of medical polymer materials, and provides a high-blood-compatibility PMP (polymethylpyrrolidone) membrane as well as a preparation method and application thereof in order to solve the technical problems of serious protein adhesion and poor blood compatibility caused by hydrophobicity of an existing PMP (poly-4-methyl-1-pentene) membrane. The preparation method comprises the following steps: firstly, carrying out plasma activation treatment on the PMP base film; then, a stable hydrophilic coating is constructed on the surface of the membrane through coordination of tannic acid and ferric ions and electrostatic adsorption of polyethyleneimine; and finally, carrying out low surface energy grafting modification by using methyl triethoxysilane. According to the invention, the PMP membrane with excellent anticoagulant property and high gas flux is successfully prepared through synergistic modification of the three steps. The hemolysis rate is less than or equal to 0.7%, the BSA adsorption quantity is less than or equal to 320 ng / cm < 2 >, the platelet adhesion quantity is obviously reduced, meanwhile, the oxygen flux retention rate is more than or equal to 92% (140-155 mL * min <-1 > * m <-2 >), and the core performance requirements of ECMO and other medical equipment on the membrane material are completely met. The method is simple in process, easily available in raw materials and suitable for large-scale production.

This invention provides a multifunctional electrospun material and its application, as well as an artificial blood vessel and its preparation method. The electrospun material includes an electrospun material substrate and deferoxamine. This electrospun material possesses anti-inflammatory, antioxidant, antibacterial, and anticoagulant functions. When applied to the preparation of artificial blood vessels, it can improve the regenerative capacity of blood vessels, especially by protecting vascular cells through inhibiting inflammation and hyperglycemia-induced oxidative stress in diabetic and elderly patients, thereby promoting angiogenesis. The loaded deferoxamine can achieve long-term, slow release according to the environment of the transplantation site. The released deferoxamine can prevent acute thrombosis by inhibiting platelet adhesion and activation, and can also protect the microenvironment of the transplantation site by resisting oxidative stress and inflammation levels, promoting cell infiltration and regeneration, and protecting regenerated cells.

The application discloses an enhanced anti-coagulation and anti-bacterial hemodialysis hollow fiber membrane and a preparation method thereof, and belongs to the technical field of medical membrane materials. The acid activation-polydopamine coating-sulfonated grafting modification of halloysite nanotubes is carried out in the application, so that the surface sulfonic acid group density of the halloysite nanotubes reaches 0.8-2.1 mmol / g, and the Zeta potential is less than or equal to-30 mV. The modified halloysite is dispersed in a polar aprotic solvent in advance, is incorporated into a polymer matrix after ultrasonic treatment, is filtered through a sintering filter element with a diameter of less than or equal to 5 mu m, is defoamed, is spun by using a dry spraying-wet spinning method, and is prepared into a finished product through coagulation, rinsing and drying. The application constructs a heparin-like negative charge layer, so that the activated partial thromboplastin time (APTT) is greater than or equal to 55 s, platelet adhesion and protein adsorption are significantly inhibited, and the application has a broad-spectrum antibacterial performance. The rigid halloysite induces the formation of through channels, so that the pain point that a high-flux membrane is easy to damage red blood cells is solved, the process is stable, and the application is suitable for industrial production.

The invention discloses multifunctional rapid shape memory composite frozen gel as well as a preparation method and application thereof, and belongs to the technical field of medical material preparation. According to the invention, pig small intestine submucosa (SIS) and polyurethane are subjected to composite crosslinking and chemical modification to prepare the quick shape memory composite frozen gel, and the quick shape memory composite frozen gel has the following advantages: 1) self infiltration: the quick shape memory composite frozen gel has good hydrophilicity, and can quickly absorb liquid which is 40 times of the mass of the quick shape memory composite frozen gel in a dry state; (2) self-expansion is achieved, the shape memory ability is prominent, volume recovery can be rapidly achieved within 1.8 s, and the bleeding part is effectively blocked; 3) self-enhancement: the composition has procoagulant activity, significantly promotes platelet adhesion and activation, and accelerates the coagulation process of the body; and 4) self-degradation: the frozen gel does not need to be taken out for the second time after hemostasis, can be degraded in vivo, and promotes in-situ tissue repair after hemostasis. The method has a wide application prospect in the fields of operations, wound first aid and the like.

The invention provides a multifunctional electrospinning material and application thereof, an artificial blood vessel and a preparation method of the artificial blood vessel. The electrospinning material comprises an electrospinning material base material and deferoxamine. The electrospinning material has anti-inflammatory, anti-oxidation, antibacterial and anti-coagulation functions; when applied to preparation of artificial blood vessels, the compound can improve the regeneration capacity of the blood vessels, and particularly protect vascular cells by inhibiting inflammation and hyperglycemia induced oxidative stress in diabetes and elderly patients, so that the regeneration of the blood vessels is promoted. The loaded deferoxamine can be slowly released for a long time according to the environment of a transplantation site, and the released deferoxamine can avoid the formation of acute thrombus by inhibiting the adhesion and activation of platelets; meanwhile, the microenvironment of a transplantation part can be changed by resisting oxidative stress and inflammation level, infiltration and regeneration of cells are promoted, and the regenerated cells are protected.

Belongs to the field of medical materials, and particularly relates to an anticoagulant antibacterial dialysis catheter coating based on PMPC nano-microspheres and a preparation method of the anticoagulant antibacterial dialysis catheter coating. The preparation method comprises the following steps: preparing the PMPC nano microspheres, constructing the PDA / PEI coating, and then constructing the PDA / PEI / PMPC nano microsphere coating. According to the method, raw materials are easy to obtain, the process is simple and convenient, the prepared coating can achieve anticoagulation and antibiosis at the same time, protein adsorption and platelet adhesion can be remarkably reduced, complement activation is inhibited, thrombus and infection risks are reduced, the biocompatibility is good, the problems that an existing coating is single in function, has potential safety hazards and the like can be solved, and the method is suitable for hemodialysis catheters and the like. The clinical application value is relatively high.

The invention provides a rare-earth-based anticoagulant coating for a PVC pipeline and a preparation method thereof, and the preparation method comprises the following steps: uniformly mixing a rare-earth-based complex with chitosan, citric acid, glycerol and deionized water, heating to obtain a colloidal mixed system, smearing the mixed system on the inner wall of the PVC pipeline, and drying to obtain the rare-earth-based anticoagulant coating for the PVC pipeline. And cooling and curing to obtain the rare earth-based anticoagulant coating. According to the rare-earth-based anticoagulant coating for the PVC pipeline, quaternary ammonium groups (N + (CH3) 3) of chitosan quaternary ammonium salt and a coordination hydration layer of rare earth ions have a synergistic effect, so that platelet adhesion is remarkably inhibited; the quaternary ammonium group is positively charged and can compete for adsorption with electronegative glycoprotein (GP Ib / IX / V) on the surface of the blood platelet through electrostatic interaction, so that the initial adhesion of the blood platelet is reduced.

The invention relates to a zwitterionic polymer and application thereof. In particular, the present disclosure relates to a zwitterionic polymer, its use as a biocompatible implantable material coating and a method of making the coating. The coating may be used to inhibit platelet aggregation and platelet adhesion due to exposure of blood to the material surface.

The application discloses a multifunctional composite coating for a zinc-based implant, which is prepared on the surface of the zinc-based implant, and is prepared by using poly-tannic acid as a bottom layer and grafting epsilon-polylysine on the bottom layer through a covalent bond; the application also discloses a preparation method of the composite coating, which comprises the following steps: preparing a poly-tannic acid bottom layer on the surface of the zinc implant, and then grafting epsilon-polylysine to obtain a zinc-based implant with the multifunctional composite coating, and the zinc-based implant is applied to the field of implants related to biological and pharmaceutical materials. The multifunctional composite coating can induce platelet adhesion and activation, trigger a blood coagulation cascade reaction, and quickly form a stable blood coagulation layer to provide an ideal microenvironment for bone repair; meanwhile, the introduction of epsilon-polylysine enables the coating to have a strong and long-lasting contact antibacterial capacity, and the two can synergistically promote early osteogenesis; the preparation process is simple, the conditions are mild, the raw materials have good biocompatibility, and the multifunctional composite coating is suitable for surface functionalization modification of various implant instruments such as zinc-based bone nails, bone plates and bone scaffolds.