Method for preparing tissue engineering blood vessel based on 3D bioprinting technology

A tissue engineering and bioprinting technology, applied in the field of bioengineering, can solve the problems of low cell density, low cell compatibility and tissue compatibility of replacement blood vessels, insufficient mechanical strength, etc., achieve good biocompatibility, improve cell Compatibility and histocompatibility, the effect of overcoming the lack of mechanical strength
CN104490489AActive Publication Date: 2015-04-08江苏知聚知识产权服务有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
江苏知聚知识产权服务有限公司
Publication Date
2015-04-08

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Abstract

The invention provides a method for preparing a tissue engineering blood vessel based on a 3D (3-Dimensional) bioprinting technology. The method comprises the following steps:alternately printing a blood vessel shape by virtue of a high-polymer material and a human renascent skin fiber cell column, and removing the high-polymer material after fusion to structure a tissue engineering blood vessel; filling late-outgrowth endothelial progenitor cells into the blood vessel, and performing in-vitro dynamic culturing for 7 days in a bioreactor to obtain a differentiated tissue engineering blood vessel, wherein the high-polymer material is one of pluronic F127, poly(N-isopropylacrylamide), methyl cellulose and sodium alginate. The blood vessel obtained by the method is high in biocompatibility and higher in mechanical property, thrombus formation resistant and platelet adhesion resistance, and can be used for repairing and replacing a damaged or disease blood vessel.
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Description

technical field

[0001] The invention belongs to bioengineering technology, and particularly relates to tissue engineered blood vessels for treating vascular diseases, that is, 3D printing is used to prepare unstented tissue engineered blood vessels, which are applied to repair or replace damaged or diseased native blood vessels. Background technique

[0002] Vascular replacement is one of the effective methods for treating vascular diseases. Commonly used replacement blood vessels are divided into autologous blood vessels, allogeneic blood vessels and tissue engineered blood vessels. The source of human autologous blood vessels is limited, and there is immune rejection of allogeneic blood vessels, so the research on the construction of tissue engineered blood vessels has become particularly important.

[0003] The basic way to construct tissue-engineered blood vessels is to use natural or synthetic materials similar to blood vessels to construct scaffolds, plant seed cells ...

Claims

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