321results about How to "Good cell compatibility" patented technology

A method to enhance osteoblast functionality of a medical implant. The method may include obtaining the medical implant and treating a surface of the medical implant to modify the surface characteristics causing increase functionality of adjacent positioned osteoblasts. A method of increasing cellular activity of a medical implant is also disclosed. A medical device having enhanced cytocompatibility capabilities includes a metallic substrate with an outer surface. Attached to the outer surface is a composition of nanosized structures. A biosensor for use with a medical device, includes an electrode that is attached to an outer surface of the medical device. The biosensor measures electrochemical changes adjacent to the medical implant. Further, a method of manufacturing a medical implant with a biosensor for use in vivo and a method of integrating a biosensor with a medical implant for use in monitoring conductivity and electrochemical changes adjacent to the medical implant are disclosed.

The invention provides a a design strategy of a novel adhesive hydrogel material and obtains a high-extension, self-healing, and high-adhesion biocompatible hydrogel material. Based on a double network structure design principle, polymerizable polyethylene glycol-based high polymer constitutes a first covalent crosslinked network, and hydrophilic high polymer with ultra-high molecular weight forms a second noncovalent crosslinked network through entanglement. A mesh size of a first network is larger than a molecular effective size of second network high polymer, and molecular weigh of high polymer forming a second network should be larger than a critical entanglement molecular weight thereof, so that effective molecular entanglement is formed, high-extension, self-healing, and high-adhesion characteristics are given to double network hydrogel. The novel hydrogel can rapidly and repeatedly form stable adhesion with surfaces of various materials. Damaged by an external force, the hydrogel can rapidly and repeatedly perform self healing. The material can be used as a cell cultivating carrier, a sewing-free novel dressing, and can be applied to wearble sensors.

The invention discloses a preparation method of carbon quantum dots with a high fluorescence property by using glycine and glucose as precursors. The method comprises using the glycine as a surface passivating agent, the glucose as a carbon source material and a hydro-thermal reaction vessel as a reaction platform, carrying out a high-temperature and high-pressure hydrothermal reaction, and removing residues and water through dialysis and freeze drying to obtain carbon quantum dot powder. The preparation method of the carbon quantum dots has characteristics of simple operation, low cost and easy mass production. The prepared carbon quantum dots have excellent properties of high fluorescence property, photobleaching resistance, adjustable light emission and good biocompatibility, can be successfully applied in live cell fluorescence imaging and flow cytometry analysis, and is broad in application prospect in biomedical fields.

The invention discloses a gold nanotube based flexible stretchable electrode and a preparation method and application thereof. The preparation method comprises the following steps: performing hydrophilic treatment on a polydimethylsiloxane (PDMS) elastic film in advance and boosting uniform adhesion and fixing of a silver nanowire on the surface of an elastic base; with a disordered silver nanowire as a template, further preparing a gold nanotube with large length-diameter ratio through a replacement reaction of [Au(en)2]Cl3 and silver, thereby acquiring the gold nanotube based flexible stretchable electrode; connecting the electrode with an external electrode wire, thereby lastly acquiring an electrode used for testing. The gold nanotube based flexible stretchable electrode provided by the invention has the characteristics of high mechanical deformation resistance, excellent electrochemical property and excellent biocompatibility, can be used for monitoring mechanical sensitive cells in real time under true physiological conditions, and breaks through limitation to application of an existing nanometer material to a stretchable electrochemical transducer. The preparation method is simple and controllable and thus the electrode is easy to prepare.

The invention relates to a preparation method for a conduction and sustained release type nervous tissue engineering scaffold. The method comprises the following steps: silk fibroin Silk and lactic acid-caprolactone copolymer are dissolved in a solvent, dissolved and stirred to obtain a solution, then polyaniline and camphorsulfonic acid are added and stirred and mixed uniformly to obtain a cortex electrospinning solution, and NGF (nerve growth factors) are dissolved in ultrapure water completely to obtain a core electrospinning solution; and the cortex electrospinning solution and the core electrospinning solution are accommodated in an injector respectively for coaxial electrospinning, and then fumigation treatment and vacuum drying are performed to obtain the conduction type nervous tissue engineering scaffold. According to the prepared nanofiber scaffold, the nerve repair speed is increased from ways including external electrical stimulation (conductive polymer), biochemistry (NGFs), a topological structure required by nerve regeneration (orientation guide) and the like. The method is simple to operate, good in repeatability and high in economic benefit, and provides novel experimental thought for nerve defect repair in clinical application.

The invention relates to a preparation method and application of chitosan-based nano-fiber, in which the preparation method includes the following steps that the drugs or bio-activator such as growth factors are dissolved or dispersed in the chitisan adipic acid solution with the concentration of 0.01% to 3% (g / ml), and then the gelatin or collagen are added to dissolve. The biodegradable polyanion is dissolved in the de-ionized water and in preaparation to be the solution with the same concentration as that of the chitisan. The mixed chitisan solution is slowly added into the polyanion solution. After stirred uniformly, the nano-fiber loaded with the bio-activator is obtained through centrifuging, washing and cooling-drying. The chitosan-based nano-fiber prepared through the invention is biodegradable and has the imitated extracellular matrix structure, in which the controlled release of bio-activator can be achieved. By the adding of collagen or gelatin, the cellular compatibility of nano-fiber or the control of the release speed of the bio-activator can be improved. Therefore, the invention has a broad prospect in the fields of tissue engineering, clinical wound healing and so on.

The invention relates the method for preparing rich silver antibiotic film used for medical pyrolytic carbon and TiN film. It solves the antibiosis question. The method comprises the following steps: on the pyrolytic carbon: a buffing and cleaning; b evacuating; c sputtering and cleaning for 5 minutes with 1keV nitrogen ion; d injecting silver ion: 70, 30keV energy, 2-8mA beam current, 2X1014 / cm2-3X1014 / cm2 dosage, and forming rich silver antibiotic film and antibiosis ratio being 100%; on TiN film: a buffing and cleaning; b evacuating; c sputtering and cleaning for 5 minutes with 1keV nitrogen ion; d sputtering Ti target with 3keV, 80mA Ar+ ion, 20-40keV, beam current: bombarding with 2-8mA high-energy N+ ion for 10-20 minutes; 50-350eV, beam current: bombarding with 10-30mA low-energy N+ ion for 60-180 minutes to deposit, N2 air pressure being 8X10 3Pa; e injecting silver ion: 30-80keV, beam current: 2-8mA, 1X1017 / cm2-5X1018 / cm2 dosage, forming rich silver antibiotic film and antibiosis ratio being 90%. The film has the advantages of good abradability, strong adhesive force and anti-corrosion, good cell compatibility and long antibiosis time-effect. The film can be used to prepare artificial heart valve and hard tissue alternate material which is implanted in human.

The present invention relates to a bone substituted material. Said bone substitute material is dicalcium silicate coating layer deposited on the surface of titanium alloy of Ti-6Al-4V, said coating layer is formed from beta-Ca2SiO4 and glass phase, and its thickness if 50-400 micrometers. Its preparation method incldues the following steps: firstly, synthesizing gamma-Ca2SiO4, then using atmospheric plasma spraying process to deposit the dicalcium silicate coating layer on the titanium alloy base body, and the combination strength of said coating layer and base body is greater than 34-40 MPa.The tests show that the bone apatite can be formed on the surface of dicalcium silicate, and cell can quickly grow and proliferate on the beta-Ca2SiO4 coating layer surface, so that said substitute material possesses good biological activity and compatibility.

The invention discloses an artificial blood vessel stent with aligned fibers and a manufacturing method of the artificial blood vessel stent, and belongs to the field of tissue engineering. According to the artificial blood vessel stent provided by the invention, a bio-compatible polymer material is taken as a spinning solution; firstly, the influence of different rotating speeds on fiber alignment is researched by virtue of an electrostatic spinning method, so that a rotating speed that the best alignment degree is achieved is determined; and then, the aligned fiber artificial blood vessel stent is manufactured on the basis of the rotating speed; the artificial blood vessel stent is relatively good in biocompatibility; and in addition, the alignment of smooth muscle cells on blood vessels can be guided. The artificial blood vessel stent provided by the invention is applicable to large-scale production and is expected to be applied to actual clinical diagnosis.

The invention provides an inflammatory microenvironment responsive smart drug-loaded hydrogel and a preparation method and application thereof. The preparation method for the inflammatory microenvironment responsive smart drug-loaded hydrogel comprises the following steps: a functional polymer containing ortho-hydroxyl reacts with phenylboronic acid containing amino or carboxyl to prepare a phenylboronic acid polymer; an amphiphilic drug carrier is prepared; the prepared amphiphilic drug carrier and a hydrophobic drug are dissolved with a good solvent, and then, water is added thereinto understirring to prepare a drug-loaded micellar solution; and the hydrophilic drug and the prepared phenylboronic acid polymer are dissolved in the prepared drug-loaded micellar solution, and then, the pHvalue of a mixed solution is regulated to 8-9 to prepare the smart drug-loaded hydrogel. The hydrogel responsively releases drugs through pH and reactive oxygen, and has the advantages that the preparation components are few and the synthesis operation is simple.

The invention discloses a composite bone repairing material of a two-stage three-dimensional structure and a preparing method of the composite bone repairing material. According to the composite bone repairing material, the low-temperature rapid molding technology is used for preparing three-dimensional composite gel of a large-hole structure through a mixture of nano-hydroxyapatite, fibroin and collagen according to the designed hole diameter; and a solvent is sublimated through freezing and drying at the later period to obtain micro-holes, and therefore the bone repairing support material of the two-stage three-dimensional structure is prepared. The composite material integrates the advantages of the nano-hydroxyapatite, the fibroin and the collagen; the proportion of inorganics and organics is adjusted to improve the mechanical properties of the composite material; the proportion of the fibroin and the collagen is adjusted to control the degradation time of the composite material, so that the good bone conductivity, the good mechanical property and the biological degradability are achieved, adherence and proliferation of the osteoblast are facilitated, the bone defection curing speed is greatly increased, and the bone defection curing effect is greatly improved; and the composite bone repairing material is an ideal three-dimensional printing bone repairing material.

The invention relates to a method for preparing a porous bone scaffold by laser and increasing mechanical and biological performances of the porous bone scaffold by adding zinc oxide with little amount, which belongs to the bone tissue engineering field. Aiming at uncontrollable performances of aperture distribution, shape, space direction and connectivity in the preparation method of a traditional bone scaffold, and aiming at the disadvantages of poor mechanical property and fast resolving rate existed in tricalcium phosphate (beta-TCP), the invention provides the method for preparing the porous bone scaffold by using a selective laser sintering (SLS) technology, and provides the method for increasing performance by adding zinc oxide (ZnO). The method for preparing the porous bone scaffold has the advantages that the porous scaffold which enables three-dimensional interconnection is prepared by SLS, zinc oxide with little amount is added for introducing into a liquid phase, the particles densification can be promoted, crystal grain is refined, and the mechanical performance is improved, simultaneously, cell compatibility is increased, the degradation rate is reduced, and the biological performance is increased, so that the interconnected porous beta-TCP bone scaffold with enhanced mechanical performance and good biological performance can be finally prepared. The method has the advantages of simple preparation technology, convenient operation, low cost, easy control of technical parameter and the like.

The invention belongs to the field of medical materials, particularly relates to N,O-carboxymethyl chitosan-polyaldehyde hyaluronic acid gel and use thereof, and aims at providing an anti-adhesion blocking agent which is good in anti-adhesion effect, good in biocompatibility and bioabsorbable. The N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel provided by the invention has structural unit shown in a formula I described in the specification, wherein x=10-10,000 and y=10-10,000. The invention also provides a preparation method of the N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel, and use thereof in prevention and treatment of postoperative tissue adhesion. The prepared N,O-carboxymethyl chitosan-aldehyde hyaluronic acid gel provides a novel choice for the anti-adhesion blocking agent.

The invention discloses a biological small-diameter artificial blood vessel and preparation method thereof. The method comprises a step of crosslinking the natural extracellular matrix of vessels by using procyanidins. The small-diameter artificial blood vessel of the invention has the characteristics of improved mechanical strength and stability, non-toxicity, enzymatic degradation resistance in vivo, anti-calcification, platelet adhesion resistance, low immunogenicity, and very little protein release from cross-linking agent and the small-diameter vessel. These crosslinking characteristics are beneficial for the immigration, prolongation, and long-term anti-calcification of the small-diameter vascular smooth muscle cells and vascular endothelial cells of a human body itself after the small-diameter artificial blood vessel is implanted in the human body.

The invention provides a low-temperature quick-forming three-dimensional printing collagen silk fibroin material. The collagen silk fibroin material is prepared by, by mass, 40-80 parts of collagen, 6-45 parts of silk fibroin, 0.5-6 parts of glycerol and 0.1-5 parts of compatilizer. Adopted materials are uniform, high in biocompatibility and mechanical performance and controllable in biodegradability and can be degraded to be nontoxic and harmless substances absorbable for human bodies in different tissues in living organisms; in-vivo degrading time can be controlled by controlling a mass ratio of components so as to promote tissue regeneration.

The invention relates to a preparation method of composite hydrogel with an interpenetrating network structure. The preparation method comprises the following process steps of: (1) preparing a collagen solution with acetic acid, and preparing methacrylated chondroitin sulfate solution and methacrylated hyaluronic acid solution with deionized water, distilled water or phosphate buffer solution; (2) adjusting the pH value of the collagen solution prepared in the step (1) to 7.0-7.4 with sodium hydroxide solution under stirring at normal pressure and 0-4 DEG C, then adding the methacrylated chondroitin sulfate solution and methacrylated hyaluronic acid solution prepared in the step (1) to the collagen solution under stirring, uniformly mixing three solutions, then adding phosphate buffer solution for diluting until the collagen concentration in the mixed solution is 5-9mg / mL, then adding an initiator and a catalyst, and uniformly mixing to form a precursor solution; and (3) injecting theprecursor solution prepared in the step (2) to a mold, and placing the mold holding the precursor solution in an environment of normal pressure and 37 DEG C for 30-60minutes.

The invention discloses a bioactive ceramic fiber composite scaffold for bone repair and a preparation method of the composite scaffold, and relates to the field of bone repair materials. In order toovercome the shortcomings of large brittleness, difficulty in processing, unsatisfactory degradability and biological activity and the like of a traditional ceramic scaffold material, bioactive ceramics such as calcium silicate serve as a scaffold body, and the surfaces of ceramic fibers are uniformly coated with a layer of degradable biomedical high polymer materials by an impregnation-centrifugation-drying process to obtain a high-porosity, good-mechanical-property and biodegradable bioactive ceramic fiber composite scaffold material for bone repair. The bioactive ceramic materials are easily subjected to synostosis with host bones, inorganic ions released by degrading the bioactive ceramic materials can participate in and promote osteogenesis and even angiogenesis metabolism activity ofa body and have irritating or inducing functions for tissue regeneration repair, and defective bone tissue repair and functional reconstruction are promoted. Degradation products of the biomedical high polymer materials on the surface of the scaffold are harmless and can be excreted to the outside of the body by metabolism.

The invention provides an enzyme-responsive intelligent bacterial adhesion resistant and bactericidal layer-by-layer self-assembled multi-layer film coating and a preparation method thereof. A surface-hydrophobic gel coating which is stable in a water environment is obtained through physical coating of chitosan, hyaluronic acid and a lysine solution, so that a material is endowed with bacterial adhesion resisting and contact sterilizing functions; polylysine improves the bacterial adhesion resistance of the material, so that the coating has the favorable gram-positive and gram-negative bacterial adhesion resisting and sterilizing capabilities. The preparation method is simple in process, quick, mild in condition, and easy for dip-coating, spin-coating, spray-coating and layer-by-layer self-assembly, can be implemented industrially, and has a wide application range, and the antibacterial performance, the bio-compatibility and the lubricity of the surface of medical devices can be effectively improved.

The invention relates to an organic-inorganic composite gel material for bone repair and a preparation method thereof. The composite gel material is characterized in that the organic material is formed by synthetic polymers or natural polymers; the inorganic material is formed by SiO2, CaO and P2O5 with mole fraction of 50-80%, 10-40% and 1-10% respectively; and the mass ratio of the organic material to the inorganic material is (0.1-1):1. The method is characterized by penetrating the polymer chains with biocompatibility and biodegradability into the SiO2 inorganic network structure to form the semi-interpenetrating structure. In the invention, the good blending of the organic and inorganic components at molecular level is realized, the mechanical property of the materials is simultaneously improved, and the bioactivity of the materials is improved; and the material and preparation method have important development potential in the field of bone defect repair.

The invention relates to a novel dual-layer composite bone tissue engineering scaffold and a preparation method thereof. The dual-layer bone tissue engineering scaffold comprises an outer-layer bacterial cellulose membrane and an inner-layer PLGA / MWNTs electrostatic spinning porous scaffold. The synthetic material PLGA, a natural ingredient bacterial cellulose and an inorganic ingredient multi-wall carbon nanotubes are compounded together to form the composite material by using a vacuum freeze drying process and a high-pressure electrostatic spinning method. The materials have complementary advantages, and an ideal dual-layer composite bone tissue engineering scaffold of PLGA / MWNTs / bacterial cellulose, which is excellent in performance and relatively cheap, is built. The dual-layer composite bone tissue engineering scaffold of the PLGA / MWNTs / bacterial cellulose provided by the invention is simple in preparation method, and mild in condition, has good cell biocompatibility, accords with the requirements of application inside a living body, and has a good application prospect as a novel bone tissue engineering scaffold.

The invention discloses a preparation method of chitosan / sodium alginate dual-network hydrogel, comprising the following steps of: carrying out a Schiff base reaction on a prepolymer solution composedof modified chitosan, modified sodium alginate, a photoinitiator and water to obtain single-network hydrogel; and reacting the single-network hydrogel under the irradiation of ultraviolet light to obtain secondary photo-crosslinked chitosan / sodium alginate double-network hydrogel, wherein the modified chitosan is carboxymethyl chitosan grafted with a collagen peptide; the modified sodium alginateis formylated sodium alginate modified by methacrylic acid. The collagen peptide disclosed by the invention has non-immunogenicity, and can be grafted on chitosan through an enzymatic reaction underthe condition of not using a toxic cross-linking agent, so that the cell compatibility and the cell proliferation promoting capability of the material are improved.

The invention discloses a large-aperture bone regeneration bracket material and a preparation method thereof, and belongs to the technical field of medical biomimetic material engineering. A natural porous inorganic biological material (cuttlebone) and ammonium dihydrogen phosphate undergo a full reaction at high temperature under high pressure so as to be transformed into a multi-phase biological ceramic (consisting of calcium carbonate, hydroxyapatite and calcium phosphate). The preparation method for the material is simple in process, the material source is abundant, and the cost is low. In addition, the prepared material has good biological compatibility and high compression strength, and is environmentally-friendly. The bone cell experiment shows that the material has bone conduction activity, can promote and induce bone regeneration self-repair, can be degraded step by step in vivo, is an ideal bone transplant replacement material, and has wide application range and obvious social and economic benefits in bone surgical operation.

The invention discloses a collagen-chitose or fibrin glue asymmetric support, preparing method and application, which is characterized by the following: incorporating collagen-chitose porous material layer and fibrin glue layer; arranging the fibrin glue layer on the surface of collagen-chitose porous material layer evenly; forming interface between the fibrin glue layer and the surface of collagen-chitose porous material layer. This product possesses good cell compatibility, which is fit for complexing skin construction. This invention also discloses the preparing method of the asymmetric support and application.

The invention discloses a TiN-Ag nano composite coating and a preparation and application thereof. The TiN-Ag nano composite coating is characterized by being a composite film obtained by magnetron co-sputtering deposition; and a certain substrate temperature is kept in the sputtering process, and the obtained thickness is between 0.5[mu] m and 1[mu] m. The negative bias voltage of a substrate in the preparation process is adjusted by experiments; for an obtained TiN-Ag nano composite coating, the hardness of the substrate is remarkably improved; Ag elements are uniformly distributed in a TiN film, so a contact angle of the TiN film is significantly smaller than that of a TiN film in which no Ag is doped, good hydrophilicity is obtained and cell compatibility is improved; and the TiN-Ag nano composite coating is expected to improve application of a titanium alloy in biomedicine. The material formed by the method disclosed by the invention is stable in structure, the method can be repeated, and preparation conditions are controllable.

The invention belongs to the technical fields of nanometer bio-composite materials and tissue engineering, and discloses a chitin whisker / chitosan nanofiber double strengthened biodegradable polyester fiber composite material as well as a preparation method and application thereof. The preparation method comprises the following steps: treating chitin whiskers and biodegradable polyester by adopting an electrostatic spinning method or a 3D printing forming method to obtain a chitin whisker strengthened biodegradable polyester fiber scaffold, and introducing a chitosan nanofiber network into the fiber scaffold by utilizing a thermally induced phase separation method to obtain the chitin whisker / chitosan nanofiber double strengthened biodegradable polyester fiber composite material. According to the preparation method, the composite material with the structure that the chitin whisker strengthened biodegradable polyester fiber scaffold is provided, and chitosan nanofibers penetrate in the fiber scaffold and a fiber surface layer is obtained, has the ability of good hydrophilicity, excellent mechanical properties, good cell affinity and promoting healing of bone tissues, can be applied to the biomedical field and has good application prospect by particularly serving as a bone tissue repair material.

The invention discloses a composite nanometer fiber film formed by compositing chitosan, gelatin and shape memory polyurethane; the chitosan and gelatin are prepared by the weight ratio of (0.5-2):1; the total weight of the chitosan and gelatin and the weight of the shape memory polyurethane are prepared by the ratio of (0.2-2):1; the composite nanometer fiber film preparation method comprises the following steps: dissolve the chitosan, gelatin and shape memory polyurethane into a solvent so as to obtain a homogeneous solution; using a static spinning method to process the homogeneous solution so as to obtain the composite nanometer fiber film; carrying out proper after treatment for the composite nanometer fiber film. The composite nanometer fiber film is good in cell compatibility, wide spectrum antibiosis activity, haemostasis performance, permeability, mechanical strength, shape memory function, water vapor transmittance, heat stability and imbibition rate, and can be applied as biology medical dressing.

The invention discloses a rapid hemostatic hydrogel. The rapid hemostatic hydrogel comprises acrylamide, inorganic nanoparticles, tannic acid, a crosslinking agent, an initiator and an accelerant, wherein the addition amount of the inorganic nanoparticles is 0.2-10% of the weight of the acrylamide; the addition amount of the tannic acid is 1-20% of the weight of the acrylamide; the addition amountof the crosslinking agent is 0.25-2% of the weight of the acrylamide; the addition amount of the initiator is 0.5-3% of the weight of the acrylamide; 20-40[mu]L of the accelerant is added into each 100g of the acrylamide. The rapid hemostatic hydrogel has the advantages as follows: 1, the rapid hemostatic hydrogel with wet tissue adhesion, prepared by a preparation method provided by the invention, can be firmly adhered to a body tissue without relying on an additional adhesive, an additional bandage and the like during using, can maintain good adhesion strength during repeated use, and is very easy to strip; 2, the hemostatic hydrogel material has relatively high mechanical strength, has the elongation at break as high as 2200% and the compressive strength of 10.15MPa plus or minus 0.36MPa, and can meet surgical requirements.

The invention discloses a bionic nerve repair material with a double-layer structure. The bionic nerve repair material is formed by compositing a skin layer and a core layer; the core layer is of a multichannel columnar structure and is prepared from a chitosan solution and collagen gel or chitosan-collagen gel with a gradient condensation method; the skin layer is prepared from high polymer fiberthrough cored weaving and coating; or the skin layer is prepared from the high polymer fiber through electrostatic spinning; or the skin layer is prepared from the chitosan solution and collagen gelor chitosan-collagen gel with an extrusion method. The core layer of the nerve repair material has good cell compatibility, the multichannel structure is more beneficial to growth of nerve cells, thesuture performance and radial compression performance of the material can be improved by the aid of the skin layer, and clinical operation is facilitated.