21results about How to "Good cell compatibility" patented technology

The invention discloses a preparation method and application of a multifunctional self-healing hydrogel material with antibacterial, anti-inflammatory and diabetic wound healing promotion functions. The preparation method comprises the following steps: firstly, synthesizing silver-doped carbon quantum dots (AgCD) in one step through a hydrothermal method; the preparation method comprises the following steps: firstly, preparing sodium hyaluronate (HA), secondly, oxidizing the sodium hyaluronate (HA) into oxidized hyaluronic acid (OHA) by taking sodium periodate as an oxidizing agent, and then encapsulating AgCD and metformin (Met) into hydrogel formed by Schiff base reaction between carboxymethyl chitosan (CMCS) and the oxidized hyaluronic acid (OHA). The hydrogel provided by the invention has certain adhesion, suitable capacity of absorbing wound exudate and good biocompatibility, meanwhile, due to the addition of AgCD, the hydrogel has excellent antibacterial property, and the metformin can promote angiogenesis of a wound part, so that the hydrogel can better promote healing of a diabetic wound. The hydrogel is simple and rapid in preparation method, has low biotoxicity and antibacterial property, and provides a strategic choice for promoting healing of diabetic wounds.

The invention relates to a double-layer microneedle patch and a preparation method and application thereof.The double-layer microneedle patch comprises a drug delivery unit and an adhesion unit, the drug delivery unit is a GCM-MN microneedle array, the adhesion unit is a CSH hydrogel patch, and when the double-layer microneedle patch is used, firstly, the GCM-MN microneedle array acts on a wound through pressure, and then the CSH hydrogel patch covers the GCM-MN microneedle array. The CSH hydrogel patch is beneficial to creating a wet healing environment, has excellent adhesion stability and skin adaptability and provides an adhesion barrier for a wound, and the GCM-MN microneedle array has good cell compatibility, cell affinity, blood compatibility and biodegradability. Along with the degradation of the microneedle array at the wound, the packaged GCM nanoparticles are continuously released and go deep into the wound. Therefore, the double-layer microneedle patch disclosed by the invention has huge clinical application potential in the field of infectious wound treatment.

The invention discloses SA-GO / SiO2 composite injectable hydrogel and a preparation method and application thereof.The preparation method comprises the steps that graphene oxide (GO) powder is added into a sodium alginate (SA) solution, and the mixture is stirred to be uniform through a magnetic stirrer till the powder is completely dispersed in the solution; adding nano silicon dioxide (nSiO2) powder into the solution, and uniformly stirring by using a magnetic stirrer until the powder is completely dispersed in the solution; a CaCl2 solution is slowly dropwise added into the solution obtained in the previous step at the dropwise adding speed of 20 drops per minute, and SA-GO / SiO2 composite hydrogel containing SiO2 with different concentrations is formed through Ca < 2 + > crosslinking and can be used as an injectable biological scaffold material for bone tissue repair, so that proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells are promoted, and the bone tissue repair efficiency is improved.

The present invention relates to the technical field of biomaterial preparation, and in particular to an adhesive hydrogel with anti-inflammatory, antioxidant and pro-angiogenic capabilities and its application. The adhesive hydrogel of the present invention comprises an anti-adhesion layer, an adhesion layer and an inner layer arranged in sequence; the anti-adhesion layer and the adhesion layer are respectively formed by cross-linking ACG solutions of different pH values; the inner layer uses methacrylate whey protein as a base material and is loaded with EGCG@DEX‑PBAP. The adhesive hydrogel can be used for the repair of skin damage, muscle damage, nerve damage, and myocardial damage tissue.

This invention discloses a method for preparing and applying an antibacterial composite coating based on quaternized zinc oxide nanoparticles. The preparation method includes: grafting quaternary ammonium salt compounds onto the surface of zinc oxide nanoparticles to prepare synergistic antibacterial nanoparticles; modifying the substrate surface by co-deposition of dopamine and polyethyleneimine to introduce active functional groups; and spraying or dipping the quaternized zinc oxide nanoparticles onto the modified substrate surface to obtain the antibacterial composite coating. This novel antibacterial material preparation scheme can integrate the advantages of zinc oxide nanoparticles and quaternary ammonium salts, while solving key technical problems such as nanoparticle aggregation, weak interfacial bonding, and limited antibacterial efficiency. It is of great significance for promoting the upgrading of hygiene and safety in the fields of medicine, food, and building materials.

The invention relates to the technical field of hydrogel application, in particular to a photopolymerization rapid prototyping transparent medical hydrogel application and a preparation method thereof. The invention discloses a transparent medical hydrogel patch capable of being rapidly formed through photopolymerization. The transparent medical hydrogel patch comprises hydrogel, the hydrogel is prepared by the following steps: coating a release film with a pre-polymerization liquid containing ultraviolet light activity, and carrying out polymerization molding through ultraviolet light irradiation. According to the transparent medical hydrogel patch, vinyl monomers serve as raw materials, the hydrogel patch is prepared in a coating mode, the problem that leveling is difficult when the hydrogel patch is injected into a mold is solved, continuous batch production can be carried out, the hydrogel patch can be completely formed only by 5-15 s of UV polymerization time, and the light polymerization efficiency is remarkably improved.

The invention relates to a bone-promoting antibacterial biological functional material and a preparation method thereof, and belongs to the technical field of laser application. According to the method, the polished nickel-titanium alloy material is subjected to ultrasonic treatment and dried, and a self-organized composite periodic micro-ripple groove structure is prepared by utilizing a femtosecond laser direct writing technology and cooperatively regulating and controlling machining parameters such as laser flux, the moving speed of a machining translation table and the scanning interval in the machining process; performing anodic oxidation treatment to form rich nano holes on the basis of the structure, and preparing to obtain a groove-micro-ripple-micro-pore three-stage micro-nano composite structure; then, the prepared three-stage micro-nano composite structure is put into an alkaline Tris buffer solution containing dopamine hydrochloride to be coated with an agglomerated dopamine coating; and then placing the groove-micro-ripple-micro-pore three-stage micro-nano composite structure coated with the polydopamine coating in a silver nitrate solution to load nano-silver on the surface of the groove-micro-ripple-micro-pore three-stage micro-nano composite structure, and finally preparing the biological functional material with good bone combining capacity, bone growth promoting capacity and antibacterial capacity.

The invention discloses an injectable tissue adhesive and application thereof, and belongs to the technical field of tissue adhesives. The tissue adhesive is mainly formed by compounding a hydrophilic polymer and a block polymer, wherein the hydrophilic polymer is selected from at least one of polyethylene glycol, polyvinyl alcohol, gelatin, hyaluronic acid, alginic acid and chitosan; the block polymer is poloxamer; the hydrophilic polymer is composed of more than two types of hydrophilic polymers with molecular weights, the poloxamer is composed of more than two types of poloxamers, the hydrophilic polymer and the block polymer are covalently connected through a cross-linking group, and the adhesion time of tissues is remarkably prolonged through matched use of the hydrophilic polymer and the block polymer. The tissue adhesive disclosed by the invention has excellent adhesion performance, and can be used as a wound sealing dressing, a tissue anti-adhesion material and an implanted instrument coating in the field of biomedicine.

The invention belongs to the technical field of medical materials, and particularly relates to chondroitin sulfate composite repairing hydrogel as well as a preparation method and application thereof. The hydrogel is prepared from the following raw materials in parts by weight: 5 to 15 parts of gelatin, 1 to 5 parts of chondroitin sulfate, 0.5 to 2 parts of glutaraldehyde, 2 to 8 parts of glycerol and 70 to 92 parts of phosphate buffer saline. The specific preparation method comprises the following steps: adding a chondroitin sulfate solution into a gelatin solution, mixing, adding glycerol, mixing again, adding glutaraldehyde diluted by phosphate buffer saline water while stirring, degassing after stirring to obtain a hydrogel solution, pouring the hydrogel solution into a mold, standing for forming, soaking in the phosphate buffer saline water, and drying to obtain the chondroitin sulfate hydrogel. And finally, sterilizing to obtain the chondroitin sulfate composite repairing hydrogel. Through the unique component proportion and the preparation process, the contradiction between the mechanical property and the biological activity of the traditional material is solved, and the synergistic enhancement of multiple functions is also realized.

The invention relates to an application of an MUC1 inhibitor in preparation of a medicine for preventing and treating bacterial infection, in particular to an application of the MUC1 inhibitor in preparation of a preparation for improving the sensitivity of antibiotic treatment. By applying the MUC1 inhibitor, the effect of improving the sensitivity of the antibiotics for treating the bacterial infection can be achieved, the dosage of the antibiotics can be reduced by 50%, and a new strategy is provided for optimizing the dosage of the antibiotics for bacterial infection. In the application and the pharmaceutical composition provided by the invention, the MUC1 inhibitor has good cytocompatibility and no obvious cytotoxicity, and has a good application prospect in the field of biomedicine.

The invention belongs to the technical field of SPR (Surface Plasmon Resonance), and particularly relates to a surface plasmon element resonance chip for cell fixation and a preparation method thereof. The chip comprises a specific adsorption component and an anti-nonspecific adsorption component, and the specific adsorption component and the anti-nonspecific adsorption component are distributed on the surface of the chip in a layered manner and are matched with each other to realize fixation of cells on the surface of the chip and control of nonspecific adsorption. The preparation method comprises the following steps: firstly bonding long-chain alkane on the surface of the chip through Au-S to obtain the hydrophobic support layer, then grafting glucan or nanocellulose through reaction among various active groups to obtain the stable layer, and grafting adhesive protein and the like through reaction among the active groups to obtain the binding layer. Under the condition that non-specific adsorption on the surface of the chip is effectively controlled, the requirement of cell fixation is met; the surface of the chip has good cell compatibility and can provide conditions for adhesion, survival, proliferation and differentiation of cells; the fixation and survival rate of cells on the surface of the modified chip are obviously improved.

The present invention belongs to the field of new drug research and development, and specifically relates to a microneedle loaded with polyvinyl pyrrolidone-modified iridium nanoparticles and basic fibroblast growth factor and its application. The microneedle has good mechanical strength, can effectively deliver IrNP and bFGF to the epidermis and dermis, IrNP plays a strong anti-oxidative stress role, removes free radicals around hair follicles, bFGF reshapes microvessels around hair follicles and promotes vascularization, the two cooperate with each other to improve the hair follicle microenvironment, promote hair growth, and have good cell compatibility, promote endothelial cell vascularization, and resist damage caused by oxidative stressors.

The present application relates to the technical field of cosmetic raw materials, and discloses a camellia flower composite oil, a preparation method and application thereof, and a cosmetic. The preparation method of the camellia flower composite oil comprises the following steps: (1) performing enzymatic reaction on camellia oil in the presence of lipase and a solvent, and separating to obtain enzymatic camellia oil; (2) performing extraction on camellia flower powder and the enzymatic camellia oil to obtain an extraction mixture; and (3) performing solid-liquid separation on the extraction mixture to obtain the camellia flower composite oil. The camellia flower composite oil provided by the present application has high polarity, high antioxidant property, high safety and good formulation application performance, and can significantly improve the water phase carrying capacity and long-term stability of a water-in-oil emulsion.

The invention relates to the technical field of nerve repair, and discloses a hydrogen and magnesium ion pump catheter with a Jingxiang structure for peripheral nerve defect repair and a preparation method of the hydrogen and magnesium ion pump catheter. The pump guide pipe is composed of a hydrophilic inner layer, a hydrophobic outer layer and a storage cavern middle layer, the hydrophilic inner layer and the hydrophobic outer layer are made of degradable high polymer materials, and the storage cavern middle layer is made of a mixture of one or more of polylactic acid, polyglycolide or polylactic acid-glycolic acid and a magnesium-based material. The pump catheter is of a two-sided structure, the hydrophilic inner layer provides biophysical clues, the cell compatibility of the catheter is improved, adhesion and migration of nerve cells are promoted, the hydrophobic outer layer prevents adverse cells such as fibroblasts from adhering and invading damaged parts, and the magnesium ion and hydrogen storage middle layer can continuously release hydrogen and magnesium ions to play a biological role. The nerve regeneration microenvironment is improved, and the nerve injury repairing effect of the stent is improved.

The invention provides a resin carbon microsphere with an anticoagulation effect as well as a preparation method and application of the resin carbon microsphere. Vinyltriethoxysilane and acrylic acid are used as monomers, free radical polymerization reaction is initiated in the presence of azodiisobutyronitrile and triethyl phosphate, poly (vinyltriethoxysilane / acrylic acid) is obtained, the obtained polymer is covalently grafted on the surfaces of hydroxylated resin carbon microspheres by further utilizing hydrolytic condensation reaction, and the carbon microspheres are prepared. The resin carbon microsphere adsorbent with the anticoagulation effect is obtained. The adsorbent has good blood compatibility and cell compatibility, the polymer can be stably connected to the surfaces of the resin carbon microspheres under the action of covalent bonds, good anticoagulant stability is shown, and a modification material retains the adsorption capacity of original resin carbon microspheres to small and medium molecular toxins, so that the adsorption capacity of the resin carbon microspheres to the small and medium molecular toxins is improved. The adsorption capacity of macromolecular toxin lysozyme and the retention rate of bovine serum albumin are remarkably improved, good adsorption performance is shown, and potential clinical application prospects are achieved in the field of blood purification.

The invention belongs to the technical field of biomedical materials, and discloses a borneol analgesic type scald repair hydrogel material as well as a preparation method and application thereof. The preparation method comprises the following steps: under shielding gas, grafting gallic acid on polyvinyl alcohol to obtain gallic acid modified polyvinyl alcohol; the preparation method comprises the following steps: grafting 3-aminophenylboronic acid onto sodium alginate to obtain 3-aminophenylboronic acid modified sodium alginate; the preparation method comprises the following steps: coating chitosan on the surface of borneol by adopting a micro-emulsion method to obtain a modified nano borneol solution; dissolving gallic acid modified polyvinyl alcohol into the modified nano borneol solution, and uniformly mixing with the 3-aminophenylboronic acid modified sodium alginate solution and the Zn < 2 + > solution for crosslinking to obtain the borneol analgesic scald repair hydrogel material. The preparation method provided by the invention is simple to operate, and the prepared borneol analgesic scald repair hydrogel material integrates multiple functions of analgesia, self-healing, antibiosis and blood vessel formation promotion, and can effectively reduce pain at a wound and promote wound healing.

This invention discloses a surfactant-based self-adhesive electroluminescent device and its preparation method. The preparation method includes: preparing a polydimethylsiloxane film; preparing a nanocellulose suspension; mixing the nanocellulose suspension with silver nanowires to obtain a conductive composite suspension; preparing a light-emitting layer solution; spraying the conductive composite suspension onto the surface of the polydimethylsiloxane film to obtain the electrodes of the electroluminescent device; spin-coating the light-emitting layer solution onto the upper surface of several electrodes of the electroluminescent device to form a light-emitting layer; and laminating the electrodes of several electroluminescent devices onto the light-emitting layer to obtain the surfactant-based self-adhesive electroluminescent device. The electroluminescent device provided by this invention has excellent adhesion, water resistance, fatigue resistance, high tensile strength, high surface compliance, high dielectric constant, cell compatibility, and bi-lateral light emission.

The application belongs to the technical field of biomedical ceramic materials, and particularly relates to an inkjet 3D printing zirconia ceramic product with an intrinsic hydrophilic surface and a preparation method and application thereof. The preparation method of the inkjet 3D printing zirconia ceramic product provided by the application realizes extreme grain refinement of the zirconia ceramic, micro-strain introduction and generation of a trace amount of monoclinic phase by optimizing ink components, printing parameters and debinding and sintering processes, so that high surface energy and hydrophilic properties are obtained. The product exhibits excellent cell compatibility, immune regulation properties and soft and hard tissue integration ability without any subsequent surface modification treatment.

The invention relates to the technical field of medical instruments, and discloses a preparation method of cellulose-based bioactive filaments for wound repair, which comprises the following specific steps: step 1, preparing an alkali / urea / sodium zincate water solvent by using alkali (NaOH, KOH and LiOH), urea, thiourea and ZnO as raw materials, step 2, mixing cellulose and amino acid, and then heating to react for 10-120 minutes at 150-180 DEG C to obtain a reaction product; and 3, directly dissolving the reaction product in the step 2 into the alkali / urea / sodium zincate water solvent prepared in the step 1 at the dissolving temperature of-5 to 5 DEG C, then adding a mixed solution of a polysaccharide raw material, silver nitrate and chloroauric acid into the dissolved solution, standing for 8 to 18 hours at the temperature of 5 to 30 DEG C, and filtering to obtain the product. The cellulose-based chitosan / ZnO bioactive composite filament is prepared by a wet spinning one-step method, so that the antibacterial property, the cytocompatibility and the wound healing promoting effect of a dressing product produced by processes such as weaving subsequently can be greatly improved.

This invention relates to a method for preparing mulberry leaf extract through multiple fermentation, and the mulberry leaf extract and its applications, belonging to the field of cosmetic raw material extraction technology. This method, through a complex enzymatic pretreatment, three-stage specific fermentation, and ultrafiltration purification process, produces a mulberry leaf extract that is non-irritating to the skin, exhibits good safety, high bioavailability, and excellent skin penetration, demonstrating significant effects in enhancing the skin barrier, moisturizing, and reducing wrinkles.

The application belongs to the field of fluorescent probes, and discloses a kind of fluorescent probe for monitoring dynamic change of hydrogen peroxide in different subcellular organelles, preparation method and application thereof, the probe is with flavonol as parent nucleus, and hydrogen peroxide ratio type fluorescent probe PML is targeted to lysosome, and hydrogen peroxide ratio type fluorescent probe PZD is targeted to lipid droplet.The application also provides the preparation method and application of the probe.The probe provided by the application has novel structure, simple synthesis method, good recognition effect on hydrogen peroxide, especially hydrogen peroxide in subcellular organelles, fast response speed, high sensitivity, reliable inspection result, good biocompatibility, subcellular organelle positioning function, so that they can be used for monitoring hydrogen peroxide in intracellular microenvironment, and it has great significance to apply probe technology to practical related pathological model to develop the mechanism of hydrogen peroxide in its pathology, especially in the process of ferroptosis.