131 results about "Nonunion" patented technology

The methods and compositions of this invention provide a means of regenerating injured musculoskeletal tissue by inhibition of myostatin function. The invention provides methods of treating a nonunion fracture in an individual comprising delivering to the fracture via a delivery system comprising biodegradable hydrogel, a pharmacological amount of myostatin propeptide effective to inhibit myostatin function. The invention also teaches compositions useful for the treatment of non-union fracture in an individual, said compositions comprising a pharmacological amount of myostatin propeptide effective to inhibit myostatin function; and a delivery system for delivering said myostatin propeptide to said fracture, wherein the delivery system comprises a biodegradable hydrogel or biodegradable nanobeads.

Methods and devices are described for the regulation of Transforming Growth actor (TGF)-β1, β2, and / or β3 protein gene expression in bone cells and other tissues via the capacitive coupling or inductive coupling of specific and selective electric fields to the bone cells or other tissues, where the specific and selective electric fields are generated by application of specific and selective electric and electromagnetic signals to electrodes or one or more coils or other field generating device disposed with respect to the bone cells or other tissues so as to facilitate the treatment of diseased or injured bone and other tissues. By gene expression is meant the up-regulation or down-regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased bone and other tissue that include generating specific and selective electric and electromagnetic signals that generate fields in the target tissue optimized for increase of TGF-β1, β2, and / or β3 protein gene expression and exposing bone and other tissue to the fields generated by specific and selective signals so as to regulate TGF-β1, β2, and / or β3 protein gene expression in such tissue. The resulting methods and devices are useful for the targeted treatment of bone fractures, fractures at risk, delayed unions, nonunion of fractures, bone defects, spine fusions, osteonecrosis or avascular necrosis, as an adjunct to other therapies in the treatment of one or all of the above, in the treatment of osteoporosis, and in other conditions in which TGF-β1, β2, and / or β3 protein may be implicated.

The invention discloses a traditional Chinese medicine composition for treating bone fracture diseases, which is prepared into a medicine for internal use from certain weight parts of Chinese angelica, pseudo-ginseng root, rhizoma drynariae, himalayan teasel root, prepared frankincense, prepared myrrh, native copper, peach kernel, rheum officinale, eucommia bark, bletilla, crab, dragon's blood, ground beeltle, red paeony root, muskmelon seed, safflower, cutch, pangolin scales, cassia twig, szechuan lovage rhizome, medicinal cyathula root, earthworm, sappan wood, cortex albiziae, obscured homalomena rhizome, cibotiumbarometz, drgon's bones, gecko, nux vomica, common clubmoss herb, platycodon root, herb of oldham groundsel, papaya and cinnabar. The traditional Chinese medicine composition for treating bone fracture diseases belongs to a pure traditional Chinese medicinal preparation, is safe and reliable, has no toxic and side effect, has the characteristics of low price, high efficiency and quick effectiveness compared with medicaments of the same kind, and is mainly used for treating symptoms such as bone fracture, bone injury, bone crack, bone nonunion, comminuted fracture, old fracture and the like.

The present invention discloses one kind of injected tissue engineering bone and its construction and application. The injected tissue engineering bone consists of solution I and solution II, the solution I is the mixture of platelet rich plasma (PRP) and 80-100 mg / ml concentration fibrinogen solution in the ratio of 1 to 3-6, and contains marrow matrix stem cell in the concentration of 5*10<>6< / >-10*10<>7< / > / ml; and the solution II is solution mixture of calcium chloride in 40 mmol / ml concentration and thrombin in 400-1000 U / ml concentration. When the injected tissue engineering bone is used, solution I and solution II in the volume ratio of 1-9 are sucked with two syringes and injected into body to form gel with high adhesive force, with PRP accounting for 10-30 vol%. The injected tissue engineering bone is used in preparing implant for treating bone defect and bone nonunion and promoting fracture healing.

The invention relates to a preparation method and application of a fibrin glue composite recombinant human bone morphogenetic protein-2 (rhBMP-2) microsphere. The preparation method comprises the steps of: preparing a slow release microsphere with a proper particle diameter; and then constructing an rhBMP-2 / PLGA microsphere / fibrin glue composite material. The rhBMP-2 / PLGA microsphere fibrin glue composite material can be used through local injection, surgical trauma can be reduced, a healing process of bone fracture and nonunion is accelerated by continuously supplementing the local bone morphogenetic protein, thus the fibrin glue composite recombinant human bone morphogenetic protein-2 is a bone repair material with excellent degradability and osteogenic activity.

An intervertebral prosthetic disc for replacing a damaged natural disc includes upper and lower vertebral body engaging surfaces, an exterior wall and one or more generally vertically oriented bone channels of limited cross-sectional area to allow bone to form and fuse into continuous or discontinuous struts between the bodies. The stiffness of the disc, while supporting the bodies, allows the distance therebetween to narrow sufficiently when subjected to a predetermined load such as the patient standing, walking, etc., to transfer sufficient energy to the bone struts to create one or more nonunion joints or pseudoarthrosis at locations along the struts. The disc includes stops to limit the movement thereof to an amount sustainable by the disc without resulting in fatigue failure.

The invention belongs to the technical field of cell engineering, and particularly relates to a preparation method of an immortalized human cartilage endplate stem cell line and use of the immortalized human cartilage endplate stem cell line. The invention aims to solve the technical problem of providing an effective preparation method for constructing the immortalized human cartilage endplate stem cell line. The technical scheme is as follows: the preparation method for constructing the immortalized human cartilage endplate stem cell line by adopting the lentiviral transfection technology comprises the following steps: a, constructing a SV40T antigen virus vector; b, carrying out gene transfection of a human endplate stem cell on SV40T antigen; and c, passaging and sieving to obtain the immortalized human cartilage endplate stem cell line. The invention further provides the use of the immortalized human cartilage endplate stem cell line prepared by the method in preparation of bone tissue engineering materials. The immortalized human cartilage endplate stem cell line provided by the invention can be applied to preparation of a tissue-engineered bone and clinically provides a seed cell of the bone tissue engineering low in price and strong in osteogenic capability for the patients with a series of long bone defects and bone nonunion.

The invention discloses a resilient device of an external fixator in the clinical application of orthopaedics. The resilient device consists of a cylinder, a sliding axle in the cylinder, a flexible apparatus, a sliding nail body and a sliding axle stopper. An anti-spin rivet, a pressure regulation rivet, a pressure regulation nut, a tightening jack bolt, a hollow screw on the cylinder wall and apressurization hole are arranged in the radial direction on the cylinder wall, wherein, the sliding axle stopper can be provided with a stop jack bolt in the radial direction on the cylinder wall, ora stop nut or a stop fixture block can be arranged on the sliding axle; the cylinder wall can be designed to have equal inner diameters at the upper and lower parts or have the inner diameter of the upper part at the corresponding position of the sliding axle larger than that of the lower part; the flexible device can be made into a flexible capsule or a spring; and the sliding nail body is connected with the cylinder in a sliding manner. The resilient device can match with external fixators of different shapes and structures to form external flexible fixators. When a limb with a fracture suffers axial pressure, the external flexible fixator can have a slight axial movement so that an interaction force can be generated due to the slight movement at the end of fracture so as to stimulate callus to grow at the end of fracture. The resilient device can avoid osteoporosis due to stress shielding of the external fixator, quicken the fracture healing, increase the strength of healed bone and effectively prevent delayed fracture healing or nonunion.

Methods and devices are described for the regulation of Fibroblastic Growth Factor-2 mRNA and / or FGF-2 protein in bone cells and other tissues via capacitive coupling or inductive coupling of specific and selective electric and / or electromagnetic fields to the bone cells or other tissues, where the specific and selective fields are generated by application of specific and selective signals to electrodes or one or more coils or other field generating device disposed with respect to the bone cells or other tissues so as to facilitate the treatment of diseased or injured bone and other tissues. By gene expression is meant the up-regulation or down-regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased bone and other tissue that include generating specific and selective signals that generate electric and / or electromagnetic fields in the target tissue optimized for increase of FGF-2 protein gene expression and exposing bone and other tissue to the fields generated by specific and selective signals so as to regulate FGF-2 protein gene expression in such tissue. The resulting methods and devices are useful for the targeted treatment of osteoporosis, osteopenia, osteonecrosis, fresh bone fractures, fractures at risk, nonunion, bone defects, spine fusion, and / or other conditions in which FGF-2 protein has been implicated.