133 results about "Nonunion" patented technology

Methods and devices are described for the regulation of bone morphogenetic protein gene expression in bone cells via the application of fields generated by specific and selective electric and electromagnetic signals in the treatment of diseased or injured bone. By gene expression is meant the up-regulation or down-regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased bone tissue that include generating specific and selective electric and electromagnetic signals that generate fields optimized for increase of bone morphogenetic protein gene expression and exposing bone to the fields generated by specific and selective signals so as to regulate bone morphogenetic protein gene expression in such bone tissue. The resulting methods and devices are useful for the targeted treatment of bone fractures, fractures at risk, delayed unions, nonunion of fractures, bone defects, spine fusions, osteonecrosis or avascular necrosis, as an adjunct to other therapies in the treatment of one or all of the above, and in the treatment of osteoporosis.

An intervertebral prosthetic disc for replacing a damaged natural disc includes upper and lower vertebral body engaging surfaces, an exterior wall and one or more generally vertically oriented bone channels of limited cross-sectional area to allow bone to form and fuse into continuous or discontinuous struts between the bodies. The stiffness of the disc, while supporting the bodies, allows the distance therebetween to narrow sufficiently when subjected to a predetermined load such as the patient standing, walking, etc., to transfer sufficient energy to the bone struts to create one or more nonunion joints or pseudoarthrosis at locations along the struts. The disc includes stops to limit the movement thereof to an amount sustainable by the disc without resulting in fatigue failure.

The invention relates to a medicament for treating fractures and a preparation method thereof. The medicament comprises a bone-knitting plaster, a bone-knitting powder and a bone-knitting capsule, wherein the bone-knitting plaster comprises frankincense, myrrh, radix clematidis, red-rooted salvia roots, sesame oil, yellow lead, and the like; the bone-knitting powder comprises raidx astragali, Chinese angelica, licorice roots, Xueye, and the like; and the bone-knitting capsule comprises radix bupleuri, red peony, dipsacus asperoides, peach kernels, and the like. When in use, the bone-knitting plaster is used together with the bone-knitting powder or the bone-knitting capsule. The medicament can effectively treat fractures, bone joint dislocations, intervertebral disc protrusions and hyperosteogeny and have obvious treatment effect and high cure rate on symptoms such as delayedunions, nonunions, and the like. The medicament has no toxic or side effects, can be taken by both adults and children, is simple and convenient to operate and use, can be widely applied to various types of fractures and external wounds of different parts at different ages and is popular for patients.

The methods and compositions of this invention provide a means of regenerating injured musculoskeletal tissue by inhibition of myostatin function. The invention provides methods of treating a nonunion fracture in an individual comprising delivering to the fracture via a delivery system comprising biodegradable hydrogel, a pharmacological amount of myostatin propeptide effective to inhibit myostatin function. The invention also teaches compositions useful for the treatment of non-union fracture in an individual, said compositions comprising a pharmacological amount of myostatin propeptide effective to inhibit myostatin function; and a delivery system for delivering said myostatin propeptide to said fracture, wherein the delivery system comprises a biodegradable hydrogel or biodegradable nanobeads.

Methods and devices are described for the regulation of Transforming Growth actor (TGF)-β1, β2, and / or β3 protein gene expression in bone cells and other tissues via the capacitive coupling or inductive coupling of specific and selective electric fields to the bone cells or other tissues, where the specific and selective electric fields are generated by application of specific and selective electric and electromagnetic signals to electrodes or one or more coils or other field generating device disposed with respect to the bone cells or other tissues so as to facilitate the treatment of diseased or injured bone and other tissues. By gene expression is meant the up-regulation or down-regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased bone and other tissue that include generating specific and selective electric and electromagnetic signals that generate fields in the target tissue optimized for increase of TGF-β1, β2, and / or β3 protein gene expression and exposing bone and other tissue to the fields generated by specific and selective signals so as to regulate TGF-β1, β2, and / or β3 protein gene expression in such tissue. The resulting methods and devices are useful for the targeted treatment of bone fractures, fractures at risk, delayed unions, nonunion of fractures, bone defects, spine fusions, osteonecrosis or avascular necrosis, as an adjunct to other therapies in the treatment of one or all of the above, in the treatment of osteoporosis, and in other conditions in which TGF-β1, β2, and / or β3 protein may be implicated.

The invention relates to an exosome derived from human pluripotent stem cells, a preparation based on the exosome and use of the exosome and preparation, and belongs to the technical fields of cell biology, molecular biology and drug research and development. The human pluripotent stem cell exosome or the preparation based on the human pluripotent stem cell exosome has the uses of A, being an additive cultured in vitro for maintaining the stemness of adult stem cells and inhibiting the senility of the adult stem cells; B, being an additive cultured in vitro for maintaining the proliferative activity of various tissue primary culture cells; C, being applied in preparing medicine for treating bone and cartilage degenerative changes; D, being applied in preparing medicine for preventing the bone and cartilage degenerative changes; E, being applied in preparing medicine for treating orthopedic diseases such as osteonecrosis, bone nonunion, fractures, cartilage injuries, tendon injuries, frozen shoulders or tendon adhesion. Compared with the prior art, the exosome secreted by the pluripotent stem cells has powerful functions of promoting tissue cell regeneration, preventing senility andtreating bone diseases.

The invention discloses a humanization active forging bone and a preparation method thereof. The humanization active forging bone has main components of hydroxyapatite crystal, natural animal cancellous bones, particles of which the particle size is between 0.1 and 5 millimeters and having three-dimensional netlike porous structures, and a coating of which the pore-size distribution is between 50 and 600 microns and the surface is compounded with extracellular matrix synthesized and excreted by human cells. The forging bone not only has a similar structure with a human bone and is advantageous for cell ingrowth, formation of new bones, and bone structure reconstruction at a transplantation position, but also can be firmly combined with the coating of which the surface is coated with the extracellular matrix synthesized and excreted by the human cells and cell growth factors, promotes the attached growth of in vivo osteoblasts, and accelerates the formation of the new bones; and simultaneously, the forging bone has the advantages of molding randomly and not causing immunological rejection reactions, and can satisfy treatment needs of clinical bone defect and nonunion.

The present invention discloses one kind of injected bone repairing material and its preparation and application. The injected bone repairing material consists of solution I and solution II, the solution I is the mixture of platelet rich plasma (PRP) and 80-100 mg / ml concentration fibrinogen solution in the ratio of 1 to 3-6; and the solution II is solution mixture of calcium chloride in 40 mmol / ml concentration and thrombin in 400-1000 U / ml concentration. When the injected bone repairing material is used, solution I and solution II in the volume ratio of 1-9 are sucked with two syringes and injected into body to form gel with high adhesive force, with PRP accounting for 10-30 vol%. The injected bone repairing material is used in preparing implant for treating bone defect and bone nonunion and promoting fracture healing.

The invention discloses a preparation method of a natural-tissue-derived decellularized and decalcified bone material. According to the method, any bone tissue of a mammal is treated with a protease inhibitor-containing normal saline buffer, an organic solvent, Tirton X-containing PBS (phosphate buffer saline), SDS (sodium dodecyl sulfonate)-containing PBS, pancreatin-containing PBS, deoxyribonuclease-containing PBS, an EDTA (ethylene diamine tetraacetic acid) isotonic solution and ultrasonic waves, and the decellularized and decalcified bone material is obtained. Cancellous bones and cortical bones can be decellularized completely and simultaneously, the conditions are mild, damage to an ECM (extracellular matrix) is avoided, rapidness and stability are realized, and the obtained decellularized and decalcified bone material has the advantages of good biocompatibility, high plasticity, high biomechanical strength and the like and can be used for clinically repairing bone regeneration and repair disorder such as bone defects, nonunion and the like caused by various causes.

The present invention provides a functionally graded bioactive glass / ceramic composite structure or bioactive glass / ceramic / bioactive glass sandwich structure for use in such applications as damage resistant, ceramic dental implants, immediate tooth replacement, endodontic posts, orthopedic prostheses, orthopedic stems, bone substitutes, bone screws, plates, and anchors, nonunion fractures repair, alveolar ridge augmentation, missing small bone parts (e.g. fingers, toes, etc), maxilla facial reconstruction, spinal fusion, and scaffolds for bone regeneration, comprising a residual bioactive glass or glass-ceramic layer at all accessible surfaces, followed by an underlying graded glass-ceramic layer, and then an dense interior ceramic. The residual bioactive glass or glass-ceramic layer can be further transformed to a carbonate apatite (CHA) layer by immersing in calcifying solution or simulated body fluid (SBF) with electrolyte composition similar to that of serum. The interior ceramic preferably contains yttria-tetragonal zirconia polycrystal (Y-TZP) or ceria stabilized tetragonal zirconia polycrystal (Ce-TZP) or magnesia stabilized zirconia (Mg-PSZ) or calcia stabilized zirconia (Ca-PSZ) or alumina or zirconia-alumina composites. Further, the invention provides methods for making the same functionally graded bioactive glass / ceramic composite structure or graded bioactive glass / ceramic / bioactive glass sandwich structure.

Methods and devices are described for the regulation of Transforming Growth actor (TGF)-β1, β2, and / or β3 protein gene expression in bone cells and other tissues via the capacitive coupling or inductive coupling of specific and selective electric fields to the bone cells or other tissues, where the specific and selective electric fields are generated by application of specific and selective electric and electromagnetic signals to electrodes or one or more coils or other field generating device disposed with respect to the bone cells or other tissues so as to facilitate the treatment of diseased or injured bone and other tissues. By gene expression is meant the up-regulation or down-regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased bone and other tissue that include generating specific and selective electric and electromagnetic signals that generate fields in the target tissue optimized for increase of TGF-β1, β2, and / or β3 protein gene expression and exposing bone and other tissue to the fields generated by specific and selective signals so as to regulate TGF-β1, β2, and / or β3 protein gene expression in such tissue. The resulting methods and devices are useful for the targeted treatment of bone fractures, fractures at risk, delayed unions, nonunion of fractures, bone defects, spine fusions, osteonecrosis or avascular necrosis, as an adjunct to other therapies in the treatment of one or all of the above, in the treatment of osteoporosis, and in other conditions in which TGF-β1, β2, and / or β3 protein may be implicated

The invention discloses a resilient device of an external fixator in the clinical application of orthopaedics. The resilient device consists of a cylinder, a sliding axle in the cylinder, a flexible apparatus, a sliding nail body and a sliding axle stopper. An anti-spin rivet, a pressure regulation rivet, a pressure regulation nut, a tightening jack bolt, a hollow screw on the cylinder wall and a pressurization hole are arranged in the radial direction on the cylinder wall, wherein, the sliding axle stopper can be provided with a stop jack bolt in the radial direction on the cylinder wall, or a stop nut or a stop fixture block can be arranged on the sliding axle; the cylinder wall can be designed to have equal inner diameters at the upper and lower parts or have the inner diameter of the upper part at the corresponding position of the sliding axle larger than that of the lower part; the flexible device can be made into a flexible capsule or a spring; and the sliding nail body is connected with the cylinder in a sliding manner. The resilient device can match with external fixators of different shapes and structures to form external flexible fixators. When a limb with a fracture suffers axial pressure, the external flexible fixator can have a slight axial movement so that an interaction force can be generated due to the slight movement at the end of fracture so as to stimulate callus to grow at the end of fracture. The resilient device can avoid osteoporosis due to stress shielding of the external fixator, quicken the fracture healing, increase the strength of healed bone and effectively prevent delayed fracture healing or nonunion.

The invention discloses a traditional Chinese medicine composition for treating bone fracture diseases, which is prepared into a medicine for internal use from certain weight parts of Chinese angelica, pseudo-ginseng root, rhizoma drynariae, himalayan teasel root, prepared frankincense, prepared myrrh, native copper, peach kernel, rheum officinale, eucommia bark, bletilla, crab, dragon's blood, ground beeltle, red paeony root, muskmelon seed, safflower, cutch, pangolin scales, cassia twig, szechuan lovage rhizome, medicinal cyathula root, earthworm, sappan wood, cortex albiziae, obscured homalomena rhizome, cibotiumbarometz, drgon's bones, gecko, nux vomica, common clubmoss herb, platycodon root, herb of oldham groundsel, papaya and cinnabar. The traditional Chinese medicine composition for treating bone fracture diseases belongs to a pure traditional Chinese medicinal preparation, is safe and reliable, has no toxic and side effect, has the characteristics of low price, high efficiency and quick effectiveness compared with medicaments of the same kind, and is mainly used for treating symptoms such as bone fracture, bone injury, bone crack, bone nonunion, comminuted fracture, old fracture and the like.

The invention aims at disclosing a traditional Chinese medicine preparation for treating bone injuries; and consists of blood resin, safflower, woodlouse, earthworm, rhizoma drynariae, olibanum, myrrh, angelica, sapanwood, eucommia bark, pyrite, dipsacus asperoides, red peony root , sweet melon seed and calcined swine mandibular bone which are made into pharmacon with traditional Chinese medicine processing procedure. The invention is used for treating opening fracture, closed fracture, comminuted fracture, greenstick fracture, bone nonunion after operation, hematoma after being hurt and damage of articulatory synovium. The invention has the advantages of having broad spectrum activity, having small toxic and side effect, addressing both the symptoms and root causes, having significant medicine effect, removing clinical symptoms rapidly and having no rebound phenomena after healing.

The present invention discloses one kind of injected tissue engineering bone and its construction and application. The injected tissue engineering bone consists of solution I and solution II, the solution I is the mixture of platelet rich plasma (PRP) and 80-100 mg / ml concentration fibrinogen solution in the ratio of 1 to 3-6, and contains marrow matrix stem cell in the concentration of 5*10<>6< / >-10*10<>7< / > / ml; and the solution II is solution mixture of calcium chloride in 40 mmol / ml concentration and thrombin in 400-1000 U / ml concentration. When the injected tissue engineering bone is used, solution I and solution II in the volume ratio of 1-9 are sucked with two syringes and injected into body to form gel with high adhesive force, with PRP accounting for 10-30 vol%. The injected tissue engineering bone is used in preparing implant for treating bone defect and bone nonunion and promoting fracture healing.

The invention relates to a preparation method and application of a fibrin glue composite recombinant human bone morphogenetic protein-2 (rhBMP-2) microsphere. The preparation method comprises the steps of: preparing a slow release microsphere with a proper particle diameter; and then constructing an rhBMP-2 / PLGA microsphere / fibrin glue composite material. The rhBMP-2 / PLGA microsphere fibrin glue composite material can be used through local injection, surgical trauma can be reduced, a healing process of bone fracture and nonunion is accelerated by continuously supplementing the local bone morphogenetic protein, thus the fibrin glue composite recombinant human bone morphogenetic protein-2 is a bone repair material with excellent degradability and osteogenic activity.

The present invention provides an ointment for external application for curing various burns, scalds, wound ulcera, nonunion of wound and osteomyelitis with the obvious therapeutic effect, and its preparation process includes the following steps: placing 25 Chinese medicinal materials of lonicera flower, rhubarb, coptis root, phellodendron bark, gardenia fruit, Chinese ilex leaf, dried / fresh rehmannia and others as raw material in sesame oil with 360-400 deg.C, deep-frying until scorched, removing residue, adding beesmax to obtain paste-like material, cooling to 60 deg.C, addinkg cutch powder, borneol powder and musk powder, stirring them, high-temp. steam sterilizing and botting so as to obtain the invented ointment which is low in cost, can eliminate wound infection, can promote tissue regeneration, and can make healed wound have no obvious and clear scar.

The invention belongs to the field of pharmacy and medical technology and relates to a dentoidin sponginum and a preparation method thereof. The sponginum provided by the invention comprises 80-99wt% of dentoidin and 1-20wt% of collage sponge matrix, wherein the dentoidin is fixed on the collagen sponge through crosslinking in different weight ratios. The sponginum provided by the invention ensures that the dentoidin is filled into ill parts with bone defects, nonunion of bone, bone tissue atrophy, bone fracture, poor bone healing and the like, so that the dentoidin plays roles in inducing bone formation, promoting bone repair and promoting wound convergence.

The invention relates to an ectosteal reduction device, in particular to a combined ectosteal reduction device. The purpose is to solve the problems that as existing four limb long bone fracture portions are manually handled, labor intensity is high, handling time is long, the alignment line is inaccurate and short of standards, the fracture setting effect is poor, postoperation fracture is prone to be unaligned, and bones are prone to nonunion. Multiple sliding bases (B) are installed at the outer side of a first rotating ring (5) and the outer side of a second rotating ring (6) respectively, the sliding bases (B) located at the outer side of the first rotating ring (5) and the outer side of the second rotating ring (6) are arranged oppositely, and multiple mechanical hands (C) are oppositely symmetrically installed on two slide-up rods (7) and two slide-down rods (8). A lifting support plate (A), the sliding bases (B) and the mechanical hands (C) are used for positioning and clamping the fracture portion. The combined ectosteal reduction device is suitable for ectosteal reduction and alignment for four limb long bones.

The invention relates to a biological hollow cancellous bone screw which comprises a screw head, a screw body and a screw tail, wherein the screw head, the screw body and the screw tail are sequentially connected; a cavity for storing medicaments is arranged in the screw body; the cavity penetrates through the screw body and the screw tail; screw threads are arranged on the outer surface of the screw head; side holes are also arranged on the screw body; the screw tail is provided with a bolt; and the bolt is matched with the shape of the opening of the cavity at the screw tail and plugs the opening of the cavity. The biological hollow cancellous bone screw of the invention is used as an internal fixation device for a femoral neck and the like which can be fractured easily and can not heal easily and / or parts which can cause osteonecrosis easily, and can accelerate bone repair of fracture parts and prevent aseptic necrosis and nonunion of bones by injecting substances for promoting bone repair and regeneration into the cavity.

A locking fracture plate may become a dynamic locking plate with the removal of a single set screw. The set screw removal may be done at the time of the initial implantation of the fracture plate, or several weeks later after the fracture has been allowed to consolidate. The device of the present invention may allow for the set screw to be removed percutaneously under x-ray control. The dynamized plate may allow for fracture compression but not for fracture displacement (loss or reduction) by rotation or sheer. The dynamized plate, with the set screw removed, may still protect the fracture from rotation and sheer. While conventional fracture plates may rigidly hold the bones in place, thereby potentially slowing bone fusion, or, in extreme cases, resulting in nonunion of the fracture, the fracture plate of the present invention may allow for fracture compression and, therefore, permit bone healing, growth and repair.

The invention discloses a bone healing treatment instrument, which comprises a power generating device and an output electrode. The power producing device transmits series of sine wave electrical signals to the output electrode, which then produces an electric filed (frequency ranging from 80Hz to 120Hz) to take effect on the bone nonunion parts. The invention also discloses a bone healing treatment. The instrument can stimulate the bone nonunion parts with the low frequency (80Hz to 120Hz) signals, and accelerate the bone callus growing. The invention can significantly improve the partial skin pain threshold, and distend the partial telangiectasia. At the same time, the invention has good stimulation effect on the motor nerve and the muscle tissue, and accelerates the bone nonunion healing in various ways with little skin irritation.

The invention relates to surface-modified bone meal and a preparation method thereof. The preparation method comprises the following steps: pretreating natural bovine cancellous bone, then calcining and pulverizing to obtain porous hydroxyapatite particles, and modifying the surfaces of the particles by adsorbing medical biomacromolecules onto the surfaces of the particles to obtain the surface-modified bone meal. The bone meal material is superior to the conventional calcined bone meal in biocompatibility, benefits rapid adhesion and proliferation of bone cells and formation of a new bone, and shortens the repair time of a defective bone. The bone meal prepared by the preparation method provided by the invention can be applied to repair of bone tissue defects and bone nonunion and can beused as an alveolar filling material and the like.

The invention discloses a porous tantalum metal bone connector which is applicable to treatment of limb fracture nonunion, bone nonunion, bone defects and the like. The porous tantalum metal bone connector comprises a body. The two ends of the body are each provided with a medullary space handle. The medullary space handles and the body are integrally formed. At least one screw hole is formed in each medullary space handle. The porous tantalum metal bone connector further comprises connection screws matched with the screw holes. According to the porous tantalum metal bone connector, the structure that the medullary space handles and the body are integrally formed is adopted, the better mechanical strength is achieved, and the risk of prosthesis breakage is effectively reduced. Screw rotating prevention grooves are formed in the medullary space handles, the bone connector and corresponding bone tissue can be fixed, and the problem that an artificial prosthesis is likely to rotate and loosen is effectively reduced. According to the porous tantalum metal bone connector, the preparation method is simple, the cost is low, implementation and clinical application are facilitated, and the requirement of a majority of patients is met.

An artificial bone is prepared from polylactic acid (1.5-2 wt. portions), hydroxy apatite (1-1.5) and decalicified bone matrix (0.5-1) through emulsion mixing method where ethyl acetate and absolute alcohol are used as solvent. Its advantages include no damage to human body and good bone transfer and induction actions. It can be used for treating dysostosis and nonunion.

The invention aims to provide a rear anatomical supporting and locking plate in the proximal tibial plateau, comprising a roughly quadrangular head with a plurality of penetrated holes and an elongated limb which is connected with the head and is provided with a plurality of penetrated holes. The invention is a novel plate developed based on the traditional plates and the AO internal fixed stent principle. The anatomical locking plate operation dispenses with prebending and massive exposure of the fracture site, minimizes injury to the soft tissues, obviously reduces the failure of implants and remarkably lowers the infection and incidence of nonunion, and bolts can be used for fixing fractures in multiple angles, thus enhancing the stability. The plate conforms to the BO internal fixation principle and has extensive application prospect and incomparable advantages with respect to complicated fracture.

In order to overcome symptoms of wound infection, short healing time, relapse and infection, easy incrustation to form scar proliferation and nonunion of wound for long treatment in conventional treatment of traumas as well as allergy to antigen of diabetics and nonunion of wounds of fragile patients, the invention provides a formula of paste for removing slough and promoting growth of tissue regeneration. The formula comprises the following traditional Chinese medicines in parts by weight: 18-22 parts of frankincense, 18-22 parts of myrrh, 13-17 parts of elephant hide, 13-17 parts of lithospermum, 18-22 parts of angelica sinensis, 23-28 parts of astragalus membranaceus, 13-18 parts of muskone, 22-27 parts of propolis, 13-17 parts of salviae miltiorrhizae and 13-17 parts of golden cypress. The forming state of the medicine is a paste or powder. The paste provided by the invention can regenerate severed fingers and heal malignant traumas and infection of soft tissues for long treatment while heals non-pathogenic traumas and pathogenic traumas as well as sport injuries. The patients do not use antibiotics which cause dysfunction of the body. The using method is simple and convenient, and no pains are generated during secondary wet treatment, so that pains of the patients are reduced, and the treatment cost and time are shortened.

The invention belongs to the technical field of cell engineering, and particularly relates to a preparation method of an immortalized human cartilage endplate stem cell line and use of the immortalized human cartilage endplate stem cell line. The invention aims to solve the technical problem of providing an effective preparation method for constructing the immortalized human cartilage endplate stem cell line. The technical scheme is as follows: the preparation method for constructing the immortalized human cartilage endplate stem cell line by adopting the lentiviral transfection technology comprises the following steps: a, constructing a SV40T antigen virus vector; b, carrying out gene transfection of a human endplate stem cell on SV40T antigen; and c, passaging and sieving to obtain the immortalized human cartilage endplate stem cell line. The invention further provides the use of the immortalized human cartilage endplate stem cell line prepared by the method in preparation of bone tissue engineering materials. The immortalized human cartilage endplate stem cell line provided by the invention can be applied to preparation of a tissue-engineered bone and clinically provides a seed cell of the bone tissue engineering low in price and strong in osteogenic capability for the patients with a series of long bone defects and bone nonunion.

The invention discloses a culture method for the composition of a periosteal biological scaffold and allogenic seed cells. The culture method comprises the following steps: the non-immunogenic periosteal biological scaffold and the seed periosteal cells are prepared; the non-immunogenic periosteal biological scaffold is sealed after low-temperature drying; after gamma-ray disinfection of the non-immunogenic periosteal biological scaffold, the seed periosteal cells, with the concentration of 1*106 per milliliter, are dripped on the surface of the disinfected scaffold; a culture medium is added after the periosteal cells gradually permeate into the non-immunogenic periosteal biological scaffold together with a suspension, the medium change is carried out every 2 to 3 days, and the in-vitro composite culture lasts for one week. The periosteal biological scaffold obtained through the method has the characteristics of complete removal of immunogenic cells, as well as good preservation of the structure and major components of extracellular matrix; effective composition of the allogenic periosteal cells and the biological scaffold can be achieved, so that a biological composite scaffold material can be provided for tissue engineering researches on bone defects and bone nonunion.